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Article: Editorial: Exploring and expanding the protein universe with non-canonical amino acids.

Fuertes, Gustavo / Sakamoto, Kensaku / Budisa, Nediljko

2023 Volume 10, Page(s) 1303286

Language	English
Publishing date	2023-10-12
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2023.1303286
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Editorial

Gustavo Fuertes / Kensaku Sakamoto / Nediljko Budisa

Frontiers in Molecular Biosciences, Vol

Exploring and expanding the protein universe with non-canonical amino acids

2023 Volume 10

Keywords	non-canonical amino acid (ncAA) ; genetic code expansion (GCE) ; stop codon suppression (SCS) ; sense codon reassignment (SCR) ; unnatural base pair (UBP) ; cell-free translation system (CFTS) ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Regulation of IL-24/IL-20R2 complex formation using photocaged tyrosines and UV light.

Pham, Phuong Ngoc / Zahradník, Jiří / Kolářová, Lucie / Schneider, Bohdan / Fuertes, Gustavo

Frontiers in molecular biosciences

2023 Volume 10, Page(s) 1214235

Abstract: Human interleukin 24 (IL-24) is a multifunctional cytokine that represents an important target for autoimmune diseases and cancer. Since the biological functions of IL-24 depend on interactions with membrane receptors, on-demand regulation of the ... ...

Abstract	Human interleukin 24 (IL-24) is a multifunctional cytokine that represents an important target for autoimmune diseases and cancer. Since the biological functions of IL-24 depend on interactions with membrane receptors, on-demand regulation of the affinity between IL-24 and its cognate partners offers exciting possibilities in basic research and may have applications in therapy. As a proof-of-concept, we developed a strategy based on recombinant soluble protein variants and genetic code expansion technology to photocontrol the binding between IL-24 and one of its receptors, IL-20R2. Screening of non-canonical
Language	English
Publishing date	2023-07-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2023.1214235
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Seroprevalence of reproductive and infectious diseases in cattle: the case of Madre de Dios in the Peruvian southeastern tropics.

Trinidad, Silvia E León / Bravo, Christian Barrantes / Narvasta, Shefferson Feijoo / Fuertes, Ethel Huamán / Trigoso, Gustavo Ampuero / Sáenz, Francys Canto / Quispe-Ccasa, Hurley A

American journal of veterinary research

2024 Volume 85, Issue 4

Abstract: Objective: The objective of this study was to determine the seroprevalence of reproductive and infectious diseases in tropical cattle in the Tambopata and Tahuamanu Provinces in the department of Madre de Dios, Peru.: Sample: 156 bovines from 7 ... ...

Abstract	Objective: The objective of this study was to determine the seroprevalence of reproductive and infectious diseases in tropical cattle in the Tambopata and Tahuamanu Provinces in the department of Madre de Dios, Peru. Sample: 156 bovines from 7 cattle farms were sampled. These farms used exclusive grazing for food and natural mating for reproduction and did not have sanitary or vaccination programs. Methods: The serum of blood samples was subjected to ELISA with commercial kits for the detection of antibodies against Neospora caninum, Mycobacterium avium subsp paratuberculosis (MAP), Leptospira interrogans, pestivirus bovine viral diarrhea virus-1, retrovirus bovine leukemia virus (BLV), orbivirus bluetongue virus (BTV), and herpesvirus bovine herpes virus-1 (BHV). The data were analyzed by means of association tests with χ2 (P < .05) and Spearman rank correlation (P < .05) in the SPSS v.15.0 software (IBM Corp). Results: A low prevalence of antibodies to L interrogans, N caninum, M avium subsp paratuberculosis, bovine viral diarrhea virus-1 was found, but it was high to BTV, BLV, and BHV (100%, 53.85%, and 72.44%, respectively). The presence of BLV and BHV was higher in the Las Piedras District, bovines less than 5 years old, and cattle with breed characteristics of zebu and crossbred (P < .01). In addition, there was a significant correlation between both infections, showing 83.3% of BLV positivity that were also BHV positive (P < .01). Clinical relevance: The high prevalence of antibodies to BTV, BHV, and BLV could be due to livestock management practices, direct contact with infected animals, and variation of the presence of vectors and natural reservoirs in the context of climate change in the tropics.
MeSH term(s)	Cattle ; Animals ; Paratuberculosis/epidemiology ; Cattle Diseases/epidemiology ; Enzootic Bovine Leukosis/epidemiology ; Bovine Virus Diarrhea-Mucosal Disease ; Peru/epidemiology ; Seroepidemiologic Studies ; Diarrhea Viruses, Bovine Viral ; Antibodies, Viral ; Antibodies, Bacterial ; Communicable Diseases/veterinary ; Reproduction ; Diarrhea Virus 1, Bovine Viral ; Leukemia Virus, Bovine ; Diarrhea/veterinary
Chemical Substances	Antibodies, Viral ; Antibodies, Bacterial
Language	English
Publishing date	2024-02-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	390796-x
ISSN	1943-5681 ; 0002-9645
ISSN (online)	1943-5681
ISSN	0002-9645
DOI	10.2460/ajvr.23.08.0177
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Article ; Online: Regulation of IL-24/IL-20R2 complex formation using photocaged tyrosines and UV light

Phuong Ngoc Pham / Jiří Zahradník / Lucie Kolářová / Bohdan Schneider / Gustavo Fuertes

Frontiers in Molecular Biosciences, Vol

2023 Volume 10

Abstract	Human interleukin 24 (IL-24) is a multifunctional cytokine that represents an important target for autoimmune diseases and cancer. Since the biological functions of IL-24 depend on interactions with membrane receptors, on-demand regulation of the affinity between IL-24 and its cognate partners offers exciting possibilities in basic research and may have applications in therapy. As a proof-of-concept, we developed a strategy based on recombinant soluble protein variants and genetic code expansion technology to photocontrol the binding between IL-24 and one of its receptors, IL-20R2. Screening of non-canonical ortho-nitrobenzyl-tyrosine (NBY) residues introduced at several positions in both partners was done by a combination of biophysical and cell signaling assays. We identified one position for installing NBY, tyrosine70 of IL-20R2, which results in clear impairment of heterocomplex assembly in the dark. Irradiation with 365-nm light leads to decaging and reconstitutes the native tyrosine of the receptor that can then associate with IL-24. Photocaged IL-20R2 may be useful for the spatiotemporal control of the JAK/STAT phosphorylation cascade.
Keywords	protein-protein interactions (PPI) ; interleukin-24 ; cytokines ; optobinders ; genetically encoded non-canonical amino acids (ncAA) ; photocaged proteins ; Biology (General) ; QH301-705.5
Subject code	612
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Animal Research in Spain: A Study of Public Perception and Attitudes.

Miguel-Batuecas, Andrea / Fuertes-Recuero, Manuel / Díaz-Regañón, David / Ortiz-Díez, Gustavo / Revuelta, Luis / De Pablo-Moreno, Juan A

Animals : an open access journal from MDPI

2023 Volume 13, Issue 12

Abstract: Since the time of Hippocrates in the 4th century BC, animal research has been extensively used for various purposes up to the present day. However, the use of animals for research has also been controversial for a long time. We report the findings of a ... ...

Abstract	Since the time of Hippocrates in the 4th century BC, animal research has been extensively used for various purposes up to the present day. However, the use of animals for research has also been controversial for a long time. We report the findings of a public, online questionnaire-based survey designed to assess the opinions of a sample of Spanish society regarding animal research. Demographic data and opinions were obtained from 806 respondents. The results indicated a high level of acceptance of animal research (73.1%). However, certain factors, such as completing the questionnaire immediately after a reading negative media report (OR = 2.41; 95%CI: 1.64-3.54;
Language	English
Publishing date	2023-06-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani13122039
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Article ; Online: Pancreatic adenocarcinoma treated with surgical resection, toceranib phosphate and firocoxib in a dog: a case report.

Fuertes-Recuero, Manuel / Vázquez-Fernández, Esther / Lizasoain-Sánz, Guillermo / Arroba-Alonso, Amanda / Sánchez-López, Alejandro / Martínez-de-Merlo, Elena / Suárez-Redondo, María / Ortiz-Diez, Gustavo

Veterinary research communications

2024

Abstract: Exocrine pancreatic carcinomas are rarely reported in dogs. A ductal pancreatic adenocarcinoma in a 10-year-old intact beagle is described in this report. The diagnosis was made based on clinical signs, imaging (abdominal ultrasound and CT scan) and ... ...

Abstract	Exocrine pancreatic carcinomas are rarely reported in dogs. A ductal pancreatic adenocarcinoma in a 10-year-old intact beagle is described in this report. The diagnosis was made based on clinical signs, imaging (abdominal ultrasound and CT scan) and histopathology. Treatment consisted of partial right lobe pancreatectomy followed by adjuvant therapy with toceranib phosphate (Palladia®) and firocoxib (Previcox®) for six months. The treatment was well tolerated, and the survival time was 445 days. To our knowledge, this is the longest survival reported in the literature for a dog diagnosed with exocrine pancreatic adenocarcinoma. The results described here may contribute to provide a better understanding about this neoplasia and potential treatment options.
Language	English
Publishing date	2024-03-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	406735-6
ISSN	1573-7446 ; 0165-7380
ISSN (online)	1573-7446
ISSN	0165-7380
DOI	10.1007/s11259-024-10349-5
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Article ; Online: Description of postoperative complications and bacterial contamination of wound soaker catheters used to administer postoperative local analgesia after mastectomy in 11 dogs: case series.

Suárez-Redondo, María / Fuertes-Recuero, Manuel / Guzmán-Soltero, Alba / Aguado, Delia / Del Carmen Martín-Espada, María / Espinel-Rupérez, Jorge / Ortiz-Diez, Gustavo

Veterinary research communications

2024

Abstract: Mastectomy is a common and painful procedure in dogs. Wound soaker catheters (WSC) are frequently used to reduce postoperative pain, including pain after mastectomy. The objectives of this case series were to describe the use of WSC for owner ... ...

Abstract	Mastectomy is a common and painful procedure in dogs. Wound soaker catheters (WSC) are frequently used to reduce postoperative pain, including pain after mastectomy. The objectives of this case series were to describe the use of WSC for owner administration of postoperative local analgesia in dogs with mammary tumors treated surgically, to identify complications associated with WSC and to determine the frequency of bacterial colonization of the catheters. Twelve WSC were placed in 11 dogs during mastectomy surgery, left in place for three days, protected by a dressing and successfully managed by owners at home. No postoperative antibiotics were administered. No complications were identified in any cases. No bacterial growth was identified on bacteriological analysis of the twelve WSC. These results suggest that the use of WSC is a safe alternative for postoperative analgesia administration following mastectomy in dogs. Future studies comparing dogs with or without WSC with a larger number of dogs are needed to further evaluate efficacy and complications.
Language	English
Publishing date	2024-04-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	406735-6
ISSN	1573-7446 ; 0165-7380
ISSN (online)	1573-7446
ISSN	0165-7380
DOI	10.1007/s11259-024-10377-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 3453: Show issues

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Article: A Spanish Survey on the Perioperative Use of Antimicrobials in Small Animals.

Otero Balda, Ignacio / Fuertes-Recuero, Manuel / Penelo Hidalgo, Silvia / Espinel Rupérez, Jorge / Lapostolle, Benoit / Ayllón-Santiago, Tania / Ortiz-Díez, Gustavo

Animals : an open access journal from MDPI

2023 Volume 13, Issue 15

Abstract: Appropriate use of perioperative antimicrobials can significantly reduce the risk of post-operative infections. However, inappropriate antimicrobial use can result in the creation of multidrug-resistant bacteria, increased costs, host flora disruption, ... ...

Abstract	Appropriate use of perioperative antimicrobials can significantly reduce the risk of post-operative infections. However, inappropriate antimicrobial use can result in the creation of multidrug-resistant bacteria, increased costs, host flora disruption, side effects and increased risk of hospital-acquired infections. This survey evaluated the current perioperative use of antimicrobials in small animals by Spanish veterinarians using a web-based questionnaire. Responses were represented using descriptive statistics and a statistical analysis of the association between demographic data and perioperative antimicrobial use was performed. Pre-operative antimicrobials were administered in clean surgery by up to 68.3% of participants, 81.0% in clean-contaminated surgery and 71.3% in dirty surgery, while in the post-operative period, antimicrobials were administered by up to 86.3% of participants in clean surgery, 93.2% in clean-contaminated surgery and 87.5% in dirty surgery. Factors considered "very important" for antimicrobial selection were the degree of wound contamination, patient immunosuppression and use of prosthesis. The most frequently used antimicrobial was beta-lactamase-resistant (or potentiated) penicillin. Post-operative antimicrobial use was associated with participants without specific surgical postgraduate training. This study highlights an overuse of antimicrobials in perioperative procedures in small animal surgery in Spain. Therefore, evidence-based guidelines and further education regarding the correct use of antimicrobial prophylaxis are recommended.
Language	English
Publishing date	2023-07-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani13152475
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Article ; Online: Animal Research in Spain

Andrea Miguel-Batuecas / Manuel Fuertes-Recuero / David Díaz-Regañón / Gustavo Ortiz-Díez / Luis Revuelta / Juan A. De Pablo-Moreno

Animals, Vol 13, Iss 2039, p

A Study of Public Perception and Attitudes

2023 Volume 2039

Abstract	Since the time of Hippocrates in the 4th century BC, animal research has been extensively used for various purposes up to the present day. However, the use of animals for research has also been controversial for a long time. We report the findings of a public, online questionnaire-based survey designed to assess the opinions of a sample of Spanish society regarding animal research. Demographic data and opinions were obtained from 806 respondents. The results indicated a high level of acceptance of animal research (73.1%). However, certain factors, such as completing the questionnaire immediately after a reading negative media report (OR = 2.41; 95%CI: 1.64–3.54; p < 0.001), being a woman (OR = 1.77; 95%CI: 1.24–2.53; p = 0.002) or having a non-scientific background (OR = 2.47; 95%CI: 1.76–3.47; p < 0.001), were associated with a tendency towards a more negative opinion. The opinions seemed to be influenced by gender, education level and by protest incidents reported in the media. Our results also indicate that a lot of information regarding animal welfare, such as care and handling protocols, along with legislation was unknown to individuals. Further, a growing popularity of companion species and opposition to animal experimentation for non-biomedical purposes were reflected in the responses obtained. The use of animals for research purposes emerged as a sensitive social issue in terms of concerns about animal ethics and welfare.
Keywords	animal research ; gender ; education level ; media incident ; survey ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
Subject code	390
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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