LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 53

Search options

  1. Article: Transposon Mutagenesis of

    Neale, Helen C / Hulin, Michelle T / Harrison, Richard J / Jackson, Robert W / Arnold, Dawn L

    Microorganisms

    2021  Volume 9, Issue 6

    Abstract: Bacterial canker ... ...

    Abstract Bacterial canker of
    Language English
    Publishing date 2021-06-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9061328
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Transposon Mutagenesis of Pseudomonas syringae Pathovars syringae and morsprunorum to Identify Genes Involved in Bacterial Canker Disease of Cherry

    Neale, Helen C. / Hulin, Michelle T. / Harrison, Richard J. / Jackson, Robert W. / Arnold, Dawn L.

    Microorganisms. 2021 June 18, v. 9, no. 6

    2021  

    Abstract: Bacterial canker of Prunus, affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae (P.s.). Strains from two pathovars—P.s. pv. syringae (Pss) and P.s. pv. ... ...

    Abstract Bacterial canker of Prunus, affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae (P.s.). Strains from two pathovars—P.s. pv. syringae (Pss) and P.s. pv. morsprunorum race 1 (PsmR1) and 2 (PsmR2)—in three phylogenetically distant clades have convergently evolved to infect Prunus. The bacteria enter woody tissues through wounds and leaf scars, causing black necrotic cankers. Symptoms are also produced on blossom, fruit and leaves. Little is known about the mechanisms P.s. uses to colonise tree hosts such as Prunus. Here, we created transposon (Tn) mutant libraries in one strain of P.s. from each of the three clades and screened the mutants on immature cherry fruit to look for changes in virulence. Mutants (242) with either reduced or enhanced virulence were detected and further characterised by in vitro screens for biofilm formation, swarming ability, and pathogenicity on leaves and cut shoots. In total, 18 genes affecting virulence were selected, and these were involved in diverse functions including motility, type III secretion, membrane transport, amino acid synthesis, DNA repair and primary metabolism. Interestingly, mutation of the effector gene, hopAU1, led to an increase in virulence of Psm R2.
    Keywords DNA repair ; Prunus ; Pseudomonas syringae ; amino acids ; apricots ; bacterial canker ; biofilm ; cherries ; fruits ; genes ; leaves ; metabolism ; mutagenesis ; mutants ; pathovars ; peaches ; phylogeny ; plant pathogens ; plums ; secretion ; transposons ; trees ; virulence
    Language English
    Dates of publication 2021-0618
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9061328
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: An improved conjugation method for Pseudomonas syringae

    Neale, Helen C / Hulin, Michelle T / Harrison, Richard J / Jackson, Robert W / Mansfield, John W / Arnold, Dawn L

    Journal of microbiological methods. 2020 Oct., v. 177

    2020  

    Abstract: In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required ...

    Abstract In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.
    Keywords Pseudomonas syringae ; developmental stages ; genetic conjugation ; genome ; mutagenesis ; transposons
    Language English
    Dates of publication 2020-10
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2020.106025
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.

    Neale, Helen C / Jackson, Robert W / Preston, Gail M / Arnold, Dawn L

    Molecular microbiology

    2018  Volume 110, Issue 3, Page(s) 444–454

    Abstract: The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106 kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrPphB, which encodes an effector protein that triggers a resistance response in ... ...

    Abstract The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106 kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrPphB, which encodes an effector protein that triggers a resistance response in certain bean cultivars. Previous studies have shown that when PPHGI-1 is excised from the bacterial chromosome, avrPphB is downregulated and therefore the pathogen avoids triggering the host's defence mechanism. Here, we investigate whether the downregulation of avrPphB is caused by the supercoiling of PPHGI-1. We also investigate the effect of a PPHGI-1-encoded type 1A topoisomerase, TopB3, on island stability and bacterial pathogenicity in the plant. Supercoiling inhibitors significantly increased the expression of avrPphB but did not affect the excision of PPHGI-1. An insertional mutant of topB3 displayed an increase in avrPphB expression and an increase in PPHGI-1 excision as well as reduced population growth in resistant and susceptible cultivars of bean. These results suggest an important role for topoisomerases in the maintenance and stability of a bacterial-encoded genomic island and demonstrate that supercoiling is involved in the downregulation of an effector gene once the island has been excised, allowing the pathogen to prevent further activation of the host defence response.
    MeSH term(s) Bacterial Proteins/biosynthesis ; Bacterial Proteins/immunology ; DNA Topoisomerases/genetics ; DNA Topoisomerases/metabolism ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; DNA, Superhelical/chemistry ; DNA, Superhelical/genetics ; Gene Expression Regulation, Bacterial ; Genomic Instability ; Genomic Islands ; Mutagenesis, Insertional ; Phaseolus/microbiology ; Plant Diseases/immunology ; Plant Diseases/microbiology ; Pseudomonas syringae/genetics ; Pseudomonas syringae/immunology ; Pseudomonas syringae/metabolism ; Pseudomonas syringae/pathogenicity ; Virulence Factors/biosynthesis ; Virulence Factors/immunology
    Chemical Substances Bacterial Proteins ; DNA, Bacterial ; DNA, Superhelical ; Virulence Factors ; avrPphB protein, Pseudomonas syringae ; DNA Topoisomerases (EC 5.99.1.-)
    Language English
    Publishing date 2018-10-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/mmi.14111
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2502: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: An improved conjugation method for Pseudomonas syringae.

    Neale, Helen C / Hulin, Michelle T / Harrison, Richard J / Jackson, Robert W / Mansfield, John W / Arnold, Dawn L

    Journal of microbiological methods

    2020  Volume 177, Page(s) 106025

    Abstract: In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required ...

    Abstract In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.
    MeSH term(s) Bacteriological Techniques/methods ; Conjugation, Genetic ; DNA, Bacterial ; Genetic Techniques ; Pseudomonas syringae/genetics ; Recombination, Genetic
    Chemical Substances DNA, Bacterial
    Language English
    Publishing date 2020-08-11
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2020.106025
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Transposon mutagenesis of pseudomonas syringae pathovars syringae and morsprunorum to identify genes involved in bacterial canker disease of cherry

    Neale, Helen C. / Hulin, Michelle T. / Harrison, Richard J. / Jackson, Robert W. / Arnold, Dawn L.

    Microorganisms

    2021  Volume 9, Issue 6

    Abstract: Bacterial canker of Prunus, affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae (P.s.). Strains from two pathovars—P.s. pv. syringae (Pss) and P.s. pv. ... ...

    Abstract Bacterial canker of Prunus, affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae (P.s.). Strains from two pathovars—P.s. pv. syringae (Pss) and P.s. pv. morsprunorum race 1 (PsmR1) and 2 (PsmR2)— in three phylogenetically distant clades have convergently evolved to infect Prunus. The bacteria enter woody tissues through wounds and leaf scars, causing black necrotic cankers. Symptoms are also produced on blossom, fruit and leaves. Little is known about the mechanisms P.s. uses to colonise tree hosts such as Prunus. Here, we created transposon (Tn) mutant libraries in one strain of P.s. from each of the three clades and screened the mutants on immature cherry fruit to look for changes in virulence. Mutants (242) with either reduced or enhanced virulence were detected and further characterised by in vitro screens for biofilm formation, swarming ability, and pathogenicity on leaves and cut shoots. In total, 18 genes affecting virulence were selected, and these were involved in diverse functions including motility, type III secretion, membrane transport, amino acid synthesis, DNA repair and primary metabolism. Interestingly, mutation of the effector gene, hopAU1, led to an increase in virulence of Psm R2.
    Keywords Bacterial canker ; Pathogenicity ; Pseudomonas syringae ; Transposon mutagenesis
    Subject code 580
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Transposon Mutagenesis of Pseudomonas syringae Pathovars syringae and morsprunorum to Identify Genes Involved in Bacterial Canker Disease of Cherry

    Helen C. Neale / Michelle T. Hulin / Richard J. Harrison / Robert W. Jackson / Dawn L. Arnold

    Microorganisms, Vol 9, Iss 1328, p

    2021  Volume 1328

    Abstract: Bacterial canker of Prunus , affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae ( P.s .). Strains from two pathovars— P.s. pv. syringae ( Pss ) and P.s . pv. ... ...

    Abstract Bacterial canker of Prunus , affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae ( P.s .). Strains from two pathovars— P.s. pv. syringae ( Pss ) and P.s . pv. morsprunorum race 1 ( Psm R1) and 2 ( Psm R2)—in three phylogenetically distant clades have convergently evolved to infect Prunus . The bacteria enter woody tissues through wounds and leaf scars, causing black necrotic cankers. Symptoms are also produced on blossom, fruit and leaves. Little is known about the mechanisms P.s. uses to colonise tree hosts such as Prunus . Here, we created transposon (Tn) mutant libraries in one strain of P.s. from each of the three clades and screened the mutants on immature cherry fruit to look for changes in virulence. Mutants (242) with either reduced or enhanced virulence were detected and further characterised by in vitro screens for biofilm formation, swarming ability, and pathogenicity on leaves and cut shoots. In total, 18 genes affecting virulence were selected, and these were involved in diverse functions including motility, type III secretion, membrane transport, amino acid synthesis, DNA repair and primary metabolism. Interestingly, mutation of the effector gene, hopAU1 , led to an increase in virulence of Psm R2.
    Keywords transposon mutagenesis ; bacterial canker ; Pseudomonas syringae ; pathogenicity ; Biology (General) ; QH301-705.5
    Subject code 580
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article: Identifying resistance in wild and ornamental cherry towards bacterial canker caused by

    Hulin, Michelle T / Vadillo Dieguez, Andrea / Cossu, Francesca / Lynn, Samantha / Russell, Karen / Neale, Helen C / Jackson, Robert W / Arnold, Dawn L / Mansfield, John W / Harrison, Richard J

    Plant pathology

    2021  Volume 71, Issue 4, Page(s) 949–965

    Abstract: Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry ( ...

    Abstract Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (
    Language English
    Publishing date 2021-12-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 415941-x
    ISSN 1365-3059 ; 0032-0862
    ISSN (online) 1365-3059
    ISSN 0032-0862
    DOI 10.1111/ppa.13513
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 945: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article: Identifying resistance in wild and ornamental cherry towards bacterial canker caused by Pseudomonas syringae

    Hulin, Michelle T. / Vadillo Dieguez, Andrea / Cossu, Francesca / Lynn, Samantha / Russell, Karen / Neale, Helen C. / Jackson, Robert W. / Arnold, Dawn L. / Mansfield, John W. / Harrison, Richard J.

    Plant pathology. 2022 May, v. 71, no. 4

    2022  

    Abstract: Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance ... ...

    Abstract Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance in sweet cherry is discernible using shoot or whole tree inoculations; however, these quantitative differences in resistance are not evident in detached leaf assays. To identify novel sources of resistance to canker, we used a rapid leaf pathogenicity test to screen a range of wild cherry, ornamental Prunus species and sweet cherry × ornamental cherry hybrids with the canker pathogens, Pseudomonas syringae pvs syringae, morsprunorum races 1 and 2, and avii. Several Prunus accessions exhibited limited symptom development following inoculation with each of the pathogens, and this resistance extended to 16 P. syringae strains pathogenic on sweet cherry and plum. Resistance was associated with reduced bacterial multiplication after inoculation, a phenotype similar to that of commercial sweet cherry towards nonhost strains of P. syringae. Progeny resulting from a cross of a resistant ornamental species Prunus incisa with susceptible sweet cherry (P. avium) exhibited resistance indicating it is an inherited trait. Identification of accessions with resistance to the major bacterial canker pathogens is the first step towards characterizing the underlying genetic mechanisms of resistance and introducing these traits into commercial germplasm.
    Keywords Prunus avium ; Prunus incisa ; Pseudomonas syringae ; bacterial canker ; cherries ; disease control ; germplasm ; leaves ; pathogenicity ; phenotype ; plant pathology ; plums ; progeny ; trees ; varietal resistance
    Language English
    Dates of publication 2022-05
    Size p. 949-965.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 415941-x
    ISSN 1365-3059 ; 0032-0862
    ISSN (online) 1365-3059
    ISSN 0032-0862
    DOI 10.1111/ppa.13513
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 945: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: An improved conjugation method for Pseudomonas syringae

    Neale, Helen C. / Hulin, Michelle T. / Harrison, Richard J. / Jackson, Robert W. / Mansfield, John W. / Arnold, Dawn L.

    Journal of Microbiological Methods

    2020  Volume 177

    Abstract: In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required ...

    Abstract In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.
    Keywords Conjugation ; Pseudomonas ; TnSeq
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top