Article ; Online: Cryo-EM of the Nucleosome Core Particle Bound to Ran-RCC1 Reveals a Dynamic Complex.
2024 Volume 63, Issue 7, Page(s) 880–892
Abstract: Ras-related nuclear protein (Ran) is a member of the Ras superfamily of small guanosine triphosphatases (GTPases) and a regulator of multiple cellular processes. In healthy cells, the GTP-bound form of Ran is concentrated at chromatin, creating a Ran•GTP ...
| Abstract | Ras-related nuclear protein (Ran) is a member of the Ras superfamily of small guanosine triphosphatases (GTPases) and a regulator of multiple cellular processes. In healthy cells, the GTP-bound form of Ran is concentrated at chromatin, creating a Ran•GTP gradient that provides the driving force for nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation. The Ran•GTP gradient is maintained by the regulator of chromatin condensation 1 (RCC1), a guanine nucleotide exchange factor that accelerates GDP/GTP exchange in Ran. RCC1 interacts with nucleosomes, which are the fundamental repeating units of eukaryotic chromatin. Here, we present a cryo-EM analysis of a trimeric complex composed of the nucleosome core particle (NCP), RCC1, and Ran. While the contacts between RCC1 and Ran in the complex are preserved compared with a previously determined structure of RCC1-Ran, our study reveals that RCC1 and Ran interact dynamically with the NCP and undergo rocking motions on the nucleosome surface. Furthermore, the switch 1 region of Ran, which plays an important role in mediating conformational changes associated with the substitution of GDP and GTP nucleotides in Ras family members, appears to undergo disorder-order transitions and forms transient contacts with the C-terminal helix of histone H2B. Nucleotide exchange assays performed in the presence and absence of NCPs are not consistent with an active role for nucleosomes in nucleotide exchange, at least |
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| MeSH term(s) | Cell Cycle Proteins/chemistry ; Cell Cycle Proteins/metabolism ; Chromatin ; Cryoelectron Microscopy ; Guanosine Triphosphate/metabolism ; Nuclear Proteins/chemistry ; Nuclear Proteins/metabolism ; Nucleosomes/chemistry ; Nucleosomes/metabolism ; Nucleotides/metabolism ; ran GTP-Binding Protein/metabolism ; Humans ; Guanine Nucleotide Exchange Factors/chemistry ; Guanine Nucleotide Exchange Factors/metabolism |
| Chemical Substances | Cell Cycle Proteins ; Chromatin ; Guanosine Triphosphate (86-01-1) ; Nuclear Proteins ; Nucleosomes ; Nucleotides ; ran GTP-Binding Protein (EC 3.6.5.2) ; RCC1 protein, human ; Guanine Nucleotide Exchange Factors |
| Language | English |
| Publishing date | 2024-03-19 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 1108-3 |
| ISSN | 1520-4995 ; 0006-2960 |
| ISSN (online) | 1520-4995 |
| ISSN | 0006-2960 |
| DOI | 10.1021/acs.biochem.3c00724 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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