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  1. Article ; Online: Effects of S-pindolol in mouse pancreatic and lung cancer cachexia models.

    Springer, Jochen / Jové, Queralt / de Lima Junior, Edson Alves / Ladrón, Natalia Álvarez / López-Soriano, Francisco Javier / Busquets, Silvia / Argiles, Josep M / Marks, Daniel L

    Journal of cachexia, sarcopenia and muscle

    2023  Volume 14, Issue 3, Page(s) 1244–1248

    Abstract: Background: It is known that S-pindolol attenuates muscle loss in animal models of cancer cachexia ... function, which is strongly compromised in cachectic animals.: Methods: Here, we tested 3 mg/kg/day of S ... LLC).: Results: Treatment of mice with 3 mg/kg/day of S-pindolol in KPC or LLC cancer cachexia ...

    Abstract Background: It is known that S-pindolol attenuates muscle loss in animal models of cancer cachexia and sarcopenia. In cancer cachexia, it also significantly reduced mortality and improved cardiac function, which is strongly compromised in cachectic animals.
    Methods: Here, we tested 3 mg/kg/day of S-pindolol in two murine cancer cachexia models: pancreatic cancer cachexia (KPC) and Lewis lung carcinoma (LLC).
    Results: Treatment of mice with 3 mg/kg/day of S-pindolol in KPC or LLC cancer cachexia models significantly attenuated the loss of body weight, including lean mass and muscle weights, leading to improved grip strength compared with placebo-treated mice. In the KPC model, treated mice lost less than half of the total weight lost by placebo (-0.9 ± 1.0 vs. -2.2 ± 1.4 g for S-pindolol and placebo, respectively, P < 0.05) and around a third of the lean mass lost by tumour-bearing controls (-0.4 ± 1.0 vs. -1.5 ± 1.5 g for S-pindolol and placebo, respectively, P < 0.05), whereas loss of fat mass was similar. In the LLC model, the gastrocnemius weight was higher in sham (108 ± 16 mg) and S-pindolol tumour-bearing (94 ± 15 mg) mice than that in placebo (83 ± 12 mg), whereas the soleus weight was only significantly higher in the S-pindolol-treated group (7.9 ± 1.7 mg) than that in placebo (6.5 ± 0.9). Grip strength was significantly improved by S-pindolol treatment (110.8 ± 16.2 vs. 93.9 ± 17.1 g for S-pindolol and placebo, respectively). A higher grip strength was observed in all groups; whereas S-pindolol-treated mice improved by 32.7 ± 18.5 g, tumour-bearing mice only show minimal improvements (7.3 ± 19.4 g, P < 0.01).
    Conclusions: S-pindolol is an important candidate for clinical development in the treatment of cancer cachexia that strongly attenuates loss of body weight and lean body mass. This was also seen in the weight of individual muscles and resulted in higher grip strength.
    MeSH term(s) Mice ; Animals ; Cachexia/drug therapy ; Cachexia/etiology ; Cachexia/pathology ; Lung Neoplasms/complications ; Lung Neoplasms/drug therapy ; Lung Neoplasms/pathology ; Muscle, Skeletal/pathology ; Carcinoma, Lewis Lung/complications ; Carcinoma, Lewis Lung/drug therapy ; Carcinoma, Lewis Lung/pathology ; Pancreas/pathology
    Language English
    Publishing date 2023-05-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2586864-0
    ISSN 2190-6009 ; 2190-5991
    ISSN (online) 2190-6009
    ISSN 2190-5991
    DOI 10.1002/jcsm.13249
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  2. Article ; Online: Pregnancy health in a multi-state U.S. population of systemically underserved patients and their children: PROMISE cohort design and baseline characteristics.

    Boone-Heinonen, Janne / Lyon-Scott, Kristin / Springer, Rachel / Schmidt, Teresa / Vesco, Kimberly K / Booman, Anna / Dinh, Dang / Fortmann, Stephen P / Foster, Byron A / Hauschildt, Jenny / Liu, Shuling / O'Malley, Jean / Palma, Amy / Snowden, Jonathan M / Stratton, Kalera / Tran, Sarah

    BMC public health

    2024  Volume 24, Issue 1, Page(s) 886

    Abstract: Background: Gestational weight gain (GWG) is a routinely monitored aspect of pregnancy health, yet critical gaps remain about optimal GWG in pregnant people from socially marginalized groups, or with pre-pregnancy body mass index (BMI) in the lower or ... ...

    Abstract Background: Gestational weight gain (GWG) is a routinely monitored aspect of pregnancy health, yet critical gaps remain about optimal GWG in pregnant people from socially marginalized groups, or with pre-pregnancy body mass index (BMI) in the lower or upper extremes. The PROMISE study aims to determine overall and trimester-specific GWG associated with the lowest risk of adverse birth outcomes and detrimental infant and child growth in these underrepresented subgroups. This paper presents methods used to construct the PROMISE cohort using electronic health record data from a network of community-based healthcare organizations and characterize the cohort with respect to baseline characteristics, longitudinal data availability, and GWG.
    Methods: We developed an algorithm to identify and date pregnancies based on outpatient clinical data for patients 15 years or older. The cohort included pregnancies delivered in 2005-2020 with gestational age between 20 weeks, 0 days and 42 weeks, 6 days; and with known height and adequate weight measures needed to examine GWG patterns. We linked offspring data from birth records and clinical records. We defined study variables with attention to timing relative to pregnancy and clinical data collection processes. Descriptive analyses characterize the sociodemographic, baseline, and longitudinal data characteristics of the cohort, overall and within BMI categories.
    Results: The cohort includes 77,599 pregnancies: 53% had incomes below the federal poverty level, 82% had public insurance, and the largest race and ethnicity groups were Hispanic (56%), non-Hispanic White (23%) and non-Hispanic Black (12%). Pre-pregnancy BMI groups included 2% underweight, 34% normal weight, 31% overweight, and 19%, 8%, and 5% Class I, II, and III obesity. Longitudinal data enable the calculation of trimester-specific GWG; e.g., a median of 2, 4, and 6 valid weight measures were available in the first, second, and third trimesters, respectively. Weekly rate of GWG was 0.00, 0.46, and 0.51 kg per week in the first, second, and third trimesters; differences in GWG between BMI groups were greatest in the second trimester.
    Conclusions: The PROMISE cohort enables characterization of GWG patterns and estimation of effects on child growth in underrepresented subgroups, ultimately improving the representativeness of GWG evidence and corresponding guidelines.
    MeSH term(s) Pregnancy ; Child ; Female ; Humans ; Infant, Newborn ; Vulnerable Populations ; Obesity/epidemiology ; Overweight/epidemiology ; Gestational Weight Gain ; Pregnancy Trimester, Third ; Body Mass Index ; Pregnancy Complications/epidemiology ; Pregnancy Outcome/epidemiology
    Language English
    Publishing date 2024-03-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041338-5
    ISSN 1471-2458 ; 1471-2458
    ISSN (online) 1471-2458
    ISSN 1471-2458
    DOI 10.1186/s12889-024-18257-8
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  3. Article ; Online: The Chitranjan S. Ranawat Award: Manipulation Under Anesthesia to Treat Postoperative Stiffness After Total Knee Arthroplasty: A Multicenter Randomized Clinical Trial.

    Abdel, Matthew P / Salmons, Harold I / Larson, Dirk R / Austin, Matthew S / Barnes, C Lowry / Bolognesi, Michael P / Della Valle, Craig J / Dennis, Douglas A / Garvin, Kevin L / Geller, Jeffrey A / Incavo, Stephen J / Lombardi, Adolph V / Peters, Christopher L / Schwarzkopf, Ran / Sculco, Peter K / Springer, Bryan D / Pagnano, Mark W / Berry, Daniel J

    The Journal of arthroplasty

    2024  

    Abstract: Background: Manipulation under anesthesia (MUA) occurs in 4% of patients after total knee arthroplasty (TKA). Anti-inflammatory medications may target arthrofibrosis pathogenesis, but the data are limited. This multicenter randomized clinical trial ... ...

    Abstract Background: Manipulation under anesthesia (MUA) occurs in 4% of patients after total knee arthroplasty (TKA). Anti-inflammatory medications may target arthrofibrosis pathogenesis, but the data are limited. This multicenter randomized clinical trial investigated the effect of adjuvant anti-inflammatory medications with MUA and physical therapy on range of motion (ROM) and outcomes.
    Methods: There were 124 patients (124 TKAs) who developed stiffness after primary TKA for osteoarthritis enrolled across 15 institutions. All received MUA when ROM was < 90° at 4 to 12 weeks postoperatively. Randomization proceeded via a permuted block design. Controls received MUA and physical therapy, while the treatment group also received one dose of pre-MUA intravenous dexamethasone (8 mg) and 14 days of oral celecoxib (200 mg). The ROM and clinical outcomes were assessed at 6 weeks and 1 year. This trial was registered with ClinicalTrials.gov.
    Results: The ROM significantly improved a mean of 46° from a pre-MUA ROM of 72 to 118° immediately after MUA (P < .001). The ROM was similar between the treatment and control groups at 6 weeks following MUA (101 versus 99°, respectively; P = .35) and at one year following MUA (108 versus 108°, respectively; P = .98). Clinical outcomes were similar at both end points.
    Conclusions: In this multicenter randomized clinical trial, the addition of intravenous dexamethasone and a short course of oral celecoxib after MUA did not improve ROM or outcomes. However, MUA provided a mean ROM improvement of 46° immediately, 28° at 6 weeks, and 37° at 1 year. Further investigation in regards to dosing, duration, and route of administration of anti-inflammatory medications remains warranted.
    Level of evidence: Level 1, RCT.
    Language English
    Publishing date 2024-02-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 632770-9
    ISSN 1532-8406 ; 0883-5403
    ISSN (online) 1532-8406
    ISSN 0883-5403
    DOI 10.1016/j.arth.2024.02.034
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The atypical β-blocker S-oxprenolol reduces cachexia and improves survival in a rat cancer cachexia model.

    Yuan, Luping / Springer, Jochen / Palus, Sandra / Busquets, Silvia / Jové, Queralt / Alves de Lima Junior, Edson / Anker, Markus S / von Haehling, Stephan / Álvarez Ladrón, Natalia / Millman, Oliver / Oosterlee, Annemijn / Szymczyk, Agata / López-Soriano, Francisco Javier / Anker, Stefan D / Coats, Andrew J S / Argiles, Josep M

    Journal of cachexia, sarcopenia and muscle

    2022  Volume 14, Issue 1, Page(s) 653–660

    Abstract: Background: Beta-blockers and selected stereoisomers of beta-blockers, like bisoprolol and S ... hepatoma rat cancer cachexia model and compared with placebo, 50 mg/kg/d S-oxprenolol (HR: 0.49, 95% CI: 0 ... that had a significant effect on body weight loss in the S-oxprenolol groups vs the same combination ...

    Abstract Background: Beta-blockers and selected stereoisomers of beta-blockers, like bisoprolol and S-pindolol (ACM-001), have been shown to be effective in preclinical cancer cachexia models. Here, we tested the efficacy of stereoisomers of oxprenolol in two preclinical models of cancer cachexia-the Yoshida AH-130 rat model and the Lewis lung carcinoma (LLC) mouse model.
    Methods and results: In the Yoshida AH130 hepatoma rat cancer cachexia model and compared with placebo, 50 mg/kg/d S-oxprenolol (HR: 0.49, 95% CI: 0.28-0.85, P = 0.012) was superior to 50 mg/kg/d R-oxprenolol (HR: 0.83, 95% CI 0.38-1.45, P = 0.51) in reducing mortality (= reaching ethical endpoints). Combination of the three doses (12.5, 25 and 50 mg/kg/d) that had a significant effect on body weight loss in the S-oxprenolol groups vs the same combination of the R-oxprenolol groups lead to a significantly improved survival of S-oxprenolol vs R-oxprenolol (HR: 1.61, 95% CI: 1.08-2.39, P = 0.0185). Interestingly, there is a clear dose dependency in S-oxprenolol-treated (5, 12.5, 25 and 50 mg/kg/d) groups, which was not observed in groups treated with R-oxprenolol. A dose-dependent attenuation of weight and lean mass loss by S-oxprenolol was seen in the Yoshida rat model, whereas R-oxprenolol had only had a significant effect on fat mass. S-oxprenolol also non-significantly reduced weight loss in the LLC model and also improved muscle function (grip strength 428 ± 25 and 539 ± 37 g/100 g body weight for placebo and S-oxprenolol, respectively). However, there was only a minor effect on quality of life indicators food intake and spontaneous activity in the Yoshida model (25 mg/kg/S-oxprenolol: 11.9 ± 2.5 g vs placebo: 4.9 ± 0.8 g, P = 0.013 and also vs 25 mg/kg/d R-oxprenolol: 7.5 ± 2.6 g, P = 0.025). Both enantiomers had no effects on cardiac dimensions and function at the doses used in this study. Western blotting of proteins involved in the anabolic/catabolic homoeostasis suggest that anabolic signalling is persevered (IGF-1 receptor, Akt) and catabolic signalling is inhibited (FXBO-10, TRAF-6) by S-pindolol, but not he R-enantiomer. Expression of glucose transporters Glut1 and Glut 4 was similar in all groups, as was AMPK.
    Conclusions: S-oxprenolol is superior to R-oxprenolol in cancer cachexia animal models and shows promise for a human application in cancer cachexia.
    MeSH term(s) Mice ; Rats ; Humans ; Animals ; Cachexia/drug therapy ; Cachexia/etiology ; Cachexia/metabolism ; Oxprenolol/therapeutic use ; Rats, Wistar ; Quality of Life ; Rats, Inbred Lew ; Adrenergic beta-Antagonists/therapeutic use ; Liver Neoplasms ; Pindolol
    Chemical Substances Oxprenolol (519MXN9YZR) ; ACM-001 COVID-19 vaccine ; Adrenergic beta-Antagonists ; Pindolol (BJ4HF6IU1D)
    Language English
    Publishing date 2022-11-08
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2586864-0
    ISSN 2190-6009 ; 2190-5991
    ISSN (online) 2190-6009
    ISSN 2190-5991
    DOI 10.1002/jcsm.13116
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  5. Article ; Online: Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health.

    Hamond, Camila / Browne, A Springer / de Wilde, Leah H / Hornsby, Richard L / LeCount, Karen / Anderson, Tammy / Stuber, Tod / Cranford, Hannah M / Browne, Stephanie K / Blanchard, Gerard / Horner, David / Taylor, Marissa L / Evans, Michael / Angeli, Nicole F / Roth, Joseph / Bisgard, Kristine M / Salzer, Johanna S / Schafer, Ilana J / Ellis, Brett R /
    Alt, David P / Schlater, Linda / Nally, Jarlath E / Ellis, Esther M

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 1132

    Abstract: ... We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 ...

    Abstract Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status was identified for 64/140 (45.7%); 49 (35.0%) were positive by dark-field microscopy, 60 (42.9%) by culture, 63 (45.0%) by fluorescent antibody testing, and 61 (43.6%) by real-time polymerase chain reaction (rtPCR). Molecular typing indicated that 48 isolates were L. borgpetersenii and 3 were L. kirschneri; the remaining nine comprised mixed species. In the single culture-negative sample that was rtPCR positive, genotyping directly from the kidney identified L. interrogans. Serotyping of L. borgpetersenii isolates identified serogroup Ballum and L. kirschneri isolates as serogroup Icterohaemorrhagiae. These results demonstrate that rodents are significant Leptospira carriers and adds to understanding the ecoepidemiology of leptospirosis in USVI.
    MeSH term(s) Animals ; Carrier State/diagnosis ; Carrier State/epidemiology ; Carrier State/microbiology ; Carrier State/transmission ; Disease Reservoirs/microbiology ; Female ; Humans ; Leptospira/genetics ; Leptospira/isolation & purification ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Leptospirosis/transmission ; Leptospirosis/veterinary ; Male ; Mice ; Molecular Typing ; Public Health ; Rats ; United States Virgin Islands/epidemiology ; Zoonoses
    Language English
    Publishing date 2022-01-21
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-04846-3
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  6. Article ; Online: Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes.

    Kathayat, Rahul S / Cao, Yang / Elvira, Pablo D / Sandoz, Patrick A / Zaballa, María-Eugenia / Springer, Maya Z / Drake, Lauren E / Macleod, Kay F / van der Goot, F Gisou / Dickinson, Bryan C

    Nature communications

    2018  Volume 9, Issue 1, Page(s) 334

    Abstract: The reversible modification of cysteine residues by thioester formation with palmitate (S ... palmitoylation) is an abundant lipid post-translational modification (PTM) in mammalian systems. S-palmitoylation ... metabolic lipids and mitochondrial regulation. However, it is unknown whether and/or how mitochondrial S ...

    Abstract The reversible modification of cysteine residues by thioester formation with palmitate (S-palmitoylation) is an abundant lipid post-translational modification (PTM) in mammalian systems. S-palmitoylation has been observed on mitochondrial proteins, providing an intriguing potential connection between metabolic lipids and mitochondrial regulation. However, it is unknown whether and/or how mitochondrial S-palmitoylation is regulated. Here we report the development of mitoDPPs, targeted fluorescent probes that measure the activity levels of "erasers" of S-palmitoylation, acyl-protein thioesterases (APTs), within mitochondria of live cells. Using mitoDPPs, we discover active S-depalmitoylation in mitochondria, in part mediated by APT1, an S-depalmitoylase previously thought to reside in the cytosol and on the Golgi apparatus. We also find that perturbation of long-chain acyl-CoA cytoplasm and mitochondrial regulatory proteins, respectively, results in selective responses from cytosolic and mitochondrial S-depalmitoylases. Altogether, this work reveals that mitochondrial S-palmitoylation is actively regulated by "eraser" enzymes that respond to alterations in mitochondrial lipid homeostasis.
    MeSH term(s) A549 Cells ; Acyl Coenzyme A/metabolism ; Fluorescent Dyes/metabolism ; HEK293 Cells ; HeLa Cells ; Humans ; Kinetics ; Lipoylation ; MCF-7 Cells ; Microscopy, Confocal ; Mitochondria/metabolism ; Mitochondrial Dynamics ; RNA Interference ; Thiolester Hydrolases/genetics ; Thiolester Hydrolases/metabolism
    Chemical Substances Acyl Coenzyme A ; Fluorescent Dyes ; LYPLA1 protein, human (EC 3.1.2.-) ; Thiolester Hydrolases (EC 3.1.2.-)
    Language English
    Publishing date 2018-01-23
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-017-02655-1
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  7. Article ; Online: Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health

    Camila Hamond / A. Springer Browne / Leah H. de Wilde / Richard L. Hornsby / Karen LeCount / Tammy Anderson / Tod Stuber / Hannah M. Cranford / Stephanie K. Browne / Gerard Blanchard / David Horner / Marissa L. Taylor / Michael Evans / Nicole F. Angeli / Joseph Roth / Kristine M. Bisgard / Johanna S. Salzer / Ilana J. Schafer / Brett R. Ellis /
    David P. Alt / Linda Schlater / Jarlath E. Nally / Esther M. Ellis

    Scientific Reports, Vol 12, Iss 1, Pp 1-

    2022  Volume 10

    Abstract: ... Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira ...

    Abstract Abstract Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status was identified for 64/140 (45.7%); 49 (35.0%) were positive by dark-field microscopy, 60 (42.9%) by culture, 63 (45.0%) by fluorescent antibody testing, and 61 (43.6%) by real-time polymerase chain reaction (rtPCR). Molecular typing indicated that 48 isolates were L. borgpetersenii and 3 were L. kirschneri; the remaining nine comprised mixed species. In the single culture-negative sample that was rtPCR positive, genotyping directly from the kidney identified L. interrogans. Serotyping of L. borgpetersenii isolates identified serogroup Ballum and L. kirschneri isolates as serogroup Icterohaemorrhagiae. These results demonstrate that rodents are significant Leptospira carriers and adds to understanding the ecoepidemiology of leptospirosis in USVI.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: A qualitative analysis of the social and cultural contexts that shape screen time use in Latino families living on the U.S.-Mexico border.

    Barroso, Cristina S / Springer, Andrew E / Ledingham, Christopher M / Kelder, Steven H

    International journal of qualitative studies on health and well-being

    2020  Volume 15, Issue 1, Page(s) 1735766

    Abstract: ... ...

    Abstract Purpose
    MeSH term(s) Adolescent ; Adult ; Child ; Culture ; Decision Making ; Emigrants and Immigrants ; Family Relations/ethnology ; Female ; Focus Groups ; Hispanic Americans ; Humans ; Male ; Parents/psychology ; Screen Time ; United States/ethnology
    Language English
    Publishing date 2020-02-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2232726-5
    ISSN 1748-2631 ; 1748-2623
    ISSN (online) 1748-2631
    ISSN 1748-2623
    DOI 10.1080/17482631.2020.1735766
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  9. Article ; Online: Distribution of Borrelia burgdorferi s.l. and Borrelia miyamotoi in Ixodes tick populations in Northern Germany, co-infections with Rickettsiales and assessment of potential influencing factors.

    Knoll, S / Springer, A / Hauck, D / Schunack, B / Pachnicke, S / Fingerle, V / Strube, C

    Medical and veterinary entomology

    2021  Volume 35, Issue 4, Page(s) 595–606

    Abstract: To determine Borrelia spp. (Spirochaetales: Spirochaetaceae) prevalence and species distribution in Northern Germany, Ixodes ticks were sampled from April to October in 2018 and 2019 by the flagging method at three locations each in five regions. ... ...

    Abstract To determine Borrelia spp. (Spirochaetales: Spirochaetaceae) prevalence and species distribution in Northern Germany, Ixodes ticks were sampled from April to October in 2018 and 2019 by the flagging method at three locations each in five regions. Analysis by quantitative real-time PCR of 3150 individual ticks revealed an overall prevalence of 30.6%, without significant differences between tick stages (31.7% positive adults, 28.6% positive nymphs). Significant differences were observed in seasonal infection rates, but not between regions, landscape types or sampling years. Analysis of co-infections with Rickettsiales indicated a negative association between Borrelia and Anaplasma phagocytophilum infection. The most frequent Borrelia species differentiated by Reverse Line Blot were B. afzelii and B. garinii/B. bavariensis, followed by B. valaisiana, B. burgdorferi sensu stricto, B. spielmanii and B. lusitaniae. Furthermore, B. miyamotoi was identified in 12.9% of differentiable samples. No effect of region nor landscape type on species composition was found, but significant variations in the distribution at the different sampling sites within a region were observed. The detected monthly fluctuations in prevalence and the differences in intra-regional Borrelia species distribution underline the importance of long-term and multi-location monitoring of Borrelia spp. in ticks as an essential part of public health assessment.
    MeSH term(s) Animals ; Borrelia ; Borrelia burgdorferi ; Borrelia burgdorferi Group ; Coinfection/epidemiology ; Coinfection/veterinary ; Germany/epidemiology ; Ixodes ; Rickettsiales
    Language English
    Publishing date 2021-06-27
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 286021-1
    ISSN 1365-2915 ; 0269-283X
    ISSN (online) 1365-2915
    ISSN 0269-283X
    DOI 10.1111/mve.12537
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