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  1. Article: The Rapidly Expanding Group of

    Libbrecht, Sasha / Van Dorpe, Jo / Creytens, David

    Diagnostics (Basel, Switzerland)

    2021  Volume 11, Issue 3

    Abstract: The classification of soft tissue tumors has evolved considerably in the last decade, largely due to advances in understanding the pathogenetic basis of many of these, sometimes rare, tumors. Deletion of Retinoblastoma 1 ( ...

    Abstract The classification of soft tissue tumors has evolved considerably in the last decade, largely due to advances in understanding the pathogenetic basis of many of these, sometimes rare, tumors. Deletion of Retinoblastoma 1 (
    Language English
    Publishing date 2021-03-03
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics11030430
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  2. Article: Hoarseness as the initial presentation of multiple myeloma.

    Colebunders, Jules / Hellemans, Stig / Ahmed, Melek / Libbrecht, Sasha / de Bruijn, Sévérine

    Clinical case reports

    2023  Volume 11, Issue 5, Page(s) e7322

    Abstract: Key clinical message: Myeloma of the thyroid cartilage is a rare but important differential diagnosis of a laryngeal mass. Although hoarseness as the initial presenting symptom in multiple myeloma is extremely rare, a clinician should always consider it. ...

    Abstract Key clinical message: Myeloma of the thyroid cartilage is a rare but important differential diagnosis of a laryngeal mass. Although hoarseness as the initial presenting symptom in multiple myeloma is extremely rare, a clinician should always consider it.
    Abstract: Multiple myeloma (MM) is a malignant plasma cell disorder characterized by an uncontrolled proliferation of monoclonal plasma cells. Although the clinical presentation at diagnosis can be quite variable, thyroid cartilage infiltration in MM is rare. Here we discuss a 65-year-old Caucasian male presenting to the ENT doctor with continuous hoarseness for 3 months. The initial clinical examination showed a tangible mass at the left lymph node level II-III. Further examination with fiber-optic laryngoscopy showed a bulging of the aryepiglottic and ventricular fold. Neck and chest CT scan revealed multiple osteolytic bone lesions in addition to the large lesion in the left thyroid cartilage. Laboratory work-up, PET-CT scan and biopsy of the thyroid cartilage were performed and eventually all confirmed the presence of a new diagnosis of IgA kappa MM. The patient was referred to the department of hematology to start with chemotherapy.
    Language English
    Publishing date 2023-05-12
    Publishing country England
    Document type Case Reports
    ZDB-ID 2740234-4
    ISSN 2050-0904
    ISSN 2050-0904
    DOI 10.1002/ccr3.7322
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  3. Article: Placental Findings in Lysosomal Storage Disease Diagnosis: A Case Report of Galactosialidosis.

    Libbrecht, Sasha / Eyskens, Francois / Declercq, Sabine / Colpaert, Cecile

    Case reports in pathology

    2020  Volume 2020, Page(s) 8181056

    Abstract: ... ...

    Abstract Introduction
    Language English
    Publishing date 2020-05-30
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2648758-5
    ISSN 2090-679X ; 2090-6781
    ISSN (online) 2090-679X
    ISSN 2090-6781
    DOI 10.1155/2020/8181056
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  4. Article ; Online: The Rapidly Expanding Group of RB1 -Deleted Soft Tissue Tumors

    Sasha Libbrecht / Jo Van Dorpe / David Creytens

    Diagnostics, Vol 11, Iss 3, p

    An Updated Review

    2021  Volume 430

    Abstract: The classification of soft tissue tumors has evolved considerably in the last decade, largely due to advances in understanding the pathogenetic basis of many of these, sometimes rare, tumors. Deletion of Retinoblastoma 1 ( RB1 ), a well-known tumor ... ...

    Abstract The classification of soft tissue tumors has evolved considerably in the last decade, largely due to advances in understanding the pathogenetic basis of many of these, sometimes rare, tumors. Deletion of Retinoblastoma 1 ( RB1 ), a well-known tumor suppressor gene, has been implicated in the tumorigenesis of a particular group of soft tissue neoplasms. This group of so-called “ RB1 -deleted soft tissue tumors” has been rapidly expanding in recent years, currently consisting of spindle cell/pleomorphic lipoma, atypical spindle cell/pleomorphic lipomatous tumor, pleomorphic liposarcoma, myofibroblastoma, cellular angiofibroma, and acral fibromyxoma. Most of these neoplasms, except pleomorphic liposarcoma, are considered benign entities and are mainly described in the older adult population. This article will review the currently known morphological, immunohistochemical, and molecular features of this heterogeneous group of mesenchymal tumors with an emphasis on differential diagnosis.
    Keywords retinoblastoma ; soft tissue tumor ; RB1 ; spindle cell/pleomorphic lipoma ; atypical spindle cell/pleomorphic lipomatous tumor ; pleomorphic liposarcoma ; Medicine (General) ; R5-920
    Subject code 571
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Direct co-registration of [

    Debacker, Jens M / Maris, Luna / Cordier, Fleur / Creytens, David / Deron, Philippe / Descamps, Benedicte / D'Asseler, Yves / De Man, Kathia / Keereman, Vincent / Libbrecht, Sasha / Schelfhout, Vanessa / Van de Vijver, Koen / Vanhove, Christian / Huvenne, Wouter

    European journal of nuclear medicine and molecular imaging

    2023  Volume 50, Issue 7, Page(s) 2127–2139

    Abstract: Purpose: Recent technical advancements in PET imaging have improved sensitivity and spatial resolution. Consequently, clinical nuclear medicine will be confronted with PET images on a previously unfamiliar resolution. To better understand [: Methods: ...

    Abstract Purpose: Recent technical advancements in PET imaging have improved sensitivity and spatial resolution. Consequently, clinical nuclear medicine will be confronted with PET images on a previously unfamiliar resolution. To better understand [
    Methods: A total of five patients diagnosed with a malignancy of the head and neck were injected with a clinical activity of [
    Results: The co-registration between the autoradiographs and their corresponding histopathology was successful in all specimens. The use of this novel methodology allowed direct comparison of autoradiography and histopathology and enabled the visualisation of uncharted heterogeneity in [
    Conclusion: We here describe a novel methodology enabling the direct co-registration of [
    Trial registration: ClinicalTrials.gov Identifier: NCT05068687.
    MeSH term(s) Humans ; Fluorodeoxyglucose F18 ; Positron Emission Tomography Computed Tomography ; Radiopharmaceuticals ; Feasibility Studies ; Neoplasms ; Positron-Emission Tomography/methods
    Chemical Substances Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; Radiopharmaceuticals
    Language English
    Publishing date 2023-03-01
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 8236-3
    ISSN 1619-7089 ; 0340-6997 ; 1619-7070
    ISSN (online) 1619-7089
    ISSN 0340-6997 ; 1619-7070
    DOI 10.1007/s00259-023-06153-z
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  6. Article ; Online: Intracranial myxoid mesenchymal tumor with EWSR1-CREB1 fusion.

    Libbrecht, Sasha / Van Der Meulen, Joni / Mondelaers, Veerle / Baert, Edward / Vande Walle, Caroline / Van Dorpe, Jo / Creytens, David

    Pathology, research and practice

    2020  Volume 216, Issue 12, Page(s) 153239

    Abstract: Myxoid mesenchymal tumor with predilection for intracranial location harboring EWSR1 fusions with CREB family transcription factors is a recently described and exceedingly rare neoplasm. While some debate still exists whether this is a true separate ... ...

    Abstract Myxoid mesenchymal tumor with predilection for intracranial location harboring EWSR1 fusions with CREB family transcription factors is a recently described and exceedingly rare neoplasm. While some debate still exists whether this is a true separate entity or a myxoid variant of angiomatoid fibrous histiocytoma, these tumors still deserve separate attention due to localization, fairly distinct histology and higher incidence in the pediatric population. Data regarding outcome of these neoplasms are still sparse in medical literature. We report a case of an intracranial myxoid tumor with EWSR1-CREB1 fusion in a 14-year-old girl.
    MeSH term(s) Adolescent ; Biomarkers, Tumor/genetics ; Brain Neoplasms/diagnostic imaging ; Brain Neoplasms/genetics ; Brain Neoplasms/pathology ; Diagnosis, Differential ; Female ; Gene Fusion ; Histiocytoma, Malignant Fibrous/diagnostic imaging ; Histiocytoma, Malignant Fibrous/genetics ; Histiocytoma, Malignant Fibrous/pathology ; Humans ; In Situ Hybridization, Fluorescence ; Oncogene Proteins, Fusion/genetics ; Predictive Value of Tests
    Chemical Substances Biomarkers, Tumor ; EWSR1-CREB1 fusion protein, human ; Oncogene Proteins, Fusion
    Language English
    Publishing date 2020-10-02
    Publishing country Germany
    Document type Case Reports
    ZDB-ID 391889-0
    ISSN 1618-0631 ; 0344-0338
    ISSN (online) 1618-0631
    ISSN 0344-0338
    DOI 10.1016/j.prp.2020.153239
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  7. Article ; Online: A rare but devastating cause of twin loss in a near-term pregnancy highlighting the features of severe SARS-CoV-2 placentitis.

    Libbrecht, Sasha / Van Cleemput, Jolien / Vandekerckhove, Linos / Colman, Sofie / Padalko, Elizaveta / Verhasselt, Bruno / Van de Vijver, Koen / Dendooven, Amélie / Dehaene, Isabelle / Van Dorpe, Jo

    Histopathology

    2021  Volume 79, Issue 4, Page(s) 674–676

    MeSH term(s) Adult ; COVID-19/diagnosis ; COVID-19/pathology ; COVID-19/virology ; Coronavirus Nucleocapsid Proteins/metabolism ; Female ; Humans ; Immunohistochemistry ; Maternal Death ; Necrosis ; Phosphoproteins/metabolism ; Placenta/pathology ; Placenta/virology ; Placenta Diseases/pathology ; Placenta Diseases/virology ; Pregnancy ; Pregnancy Complications, Infectious/pathology ; Pregnancy Complications, Infectious/virology ; Pregnancy Trimester, Third ; Pregnancy, Twin ; Risk ; SARS-CoV-2/genetics ; Trophoblasts/pathology ; Trophoblasts/virology ; Young Adult
    Chemical Substances Coronavirus Nucleocapsid Proteins ; Phosphoproteins ; nucleocapsid phosphoprotein, SARS-CoV-2
    Language English
    Publishing date 2021-07-08
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 131914-0
    ISSN 1365-2559 ; 0309-0167
    ISSN (online) 1365-2559
    ISSN 0309-0167
    DOI 10.1111/his.14402
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    Zs.A 1328: Show issues Location:
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  8. Article ; Online: Effect of Neoadjuvant Radiation Dose and Schedule on Nodal Count and Its Prognostic Impact in Stage II-III Rectal Cancer.

    Ceelen, Wim / Willaert, Wouter / Varewyck, Machteld / Libbrecht, Sasha / Goetghebeur, Els / Pattyn, Piet

    Annals of surgical oncology

    2016  Volume 23, Issue 12, Page(s) 3899–3906

    Abstract: Background: It is unknown how neoadjuvant treatment schedule affects lymph node count (LNC) and lymph node ratio (LNR) and how these correlate with overall survival (OS) in rectal cancer (RC).: Methods: Data were used from the Belgian PROCARE rectal ... ...

    Abstract Background: It is unknown how neoadjuvant treatment schedule affects lymph node count (LNC) and lymph node ratio (LNR) and how these correlate with overall survival (OS) in rectal cancer (RC).
    Methods: Data were used from the Belgian PROCARE rectal cancer registry on RC patients treated with surgery alone, short-term radiotherapy with immediate surgery (SRT), or chemoradiation with deferred surgery (CRT). The effect of neoadjuvant therapy on LNC was examined using Poisson log-linear analysis. The association of LNC and LNR with overall survival (OS) was studied using Cox proportional hazards models.
    Results: Data from 4037 patients were available. Compared with surgery alone, LNC was reduced by 12.3 % after SRT and by 31.3 % after CRT (p < 0.001). In patients with surgery alone, the probability of finding node-positive disease increased with LNC, while after SRT and CRT no increase was noted for more than 12 and 18 examined nodes, respectively. Per node examined, we found a decrease in hazard of death of 2.7 % after surgery alone and 1.5 % after SRT, but no effect after CRT. In stage III patients, the LNR but not (y)pN stage was significantly correlated with OS regardless of neoadjuvant therapy. Specifically, a LNR > 0.4 was associated with a significantly worse outcome.
    Conclusions: Nodal counts are reduced in a schedule-dependent manner by neoadjuvant treatment in RC. After chemoradiation, the LNC does not confer any prognostic information. A LNR of >0.4 is associated with a significantly worse outcome in stage III disease, regardless of neoadjuvant therapy type.
    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Chemoradiotherapy, Adjuvant ; Female ; Humans ; Lymph Nodes/radiation effects ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoadjuvant Therapy ; Neoplasm Staging ; Proportional Hazards Models ; Radiotherapy Dosage ; Radiotherapy, Adjuvant ; Rectal Neoplasms/pathology ; Rectal Neoplasms/radiotherapy ; Rectal Neoplasms/surgery ; Registries ; Retrospective Studies ; Survival Rate ; Time Factors ; Young Adult
    Language English
    Publishing date 2016-07-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1200469-8
    ISSN 1534-4681 ; 1068-9265
    ISSN (online) 1534-4681
    ISSN 1068-9265
    DOI 10.1245/s10434-016-5363-4
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    Zs.A 4042: Show issues Location:
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  9. Article ; Online: Placental Tissue Destruction and Insufficiency From COVID-19 Causes Stillbirth and Neonatal Death From Hypoxic-Ischemic Injury.

    Schwartz, David A / Avvad-Portari, Elyzabeth / Babál, Pavel / Baldewijns, Marcella / Blomberg, Marie / Bouachba, Amine / Camacho, Jessica / Collardeau-Frachon, Sophie / Colson, Arthur / Dehaene, Isabelle / Ferreres, Joan Carles / Fitzgerald, Brendan / Garrido-Pontnou, Marta / Gergis, Hazem / Hargitai, Beata / Helguera-Repetto, A Cecilia / Holmström, Sandra / Irles, Claudine Liliane / Leijonhfvud, Åsa /
    Libbrecht, Sasha / Marton, Tamás / McEntagart, Noel / Molina, James T / Morotti, Raffaella / Nadal, Alfons / Navarro, Alexandra / Nelander, Maria / Oviedo, Angelica / Otani, Andre Ricardo Oyamada / Papadogiannakis, Nikos / Petersen, Astrid C / Roberts, Drucilla J / Saad, Ali G / Sand, Anna / Schoenmakers, Sam / Sehn, Jennifer K / Simpson, Preston R / Thomas, Kristen / Valdespino-Vázquez, M Yolotzin / van der Meeren, Lotte E / Van Dorpe, Jo / Verdijk, Robert M / Watkins, Jaclyn C / Zaigham, Mehreen

    Archives of pathology & laboratory medicine

    2022  Volume 146, Issue 6, Page(s) 660–676

    Abstract: Context.—: Perinatal death is an increasingly important problem as the coronavirus disease 2019 (COVID-19) pandemic continues, but the mechanism of death has been unclear.: Objective.—: To evaluate the role of the placenta in causing stillbirth and ... ...

    Abstract Context.—: Perinatal death is an increasingly important problem as the coronavirus disease 2019 (COVID-19) pandemic continues, but the mechanism of death has been unclear.
    Objective.—: To evaluate the role of the placenta in causing stillbirth and neonatal death following maternal infection with COVID-19 and confirmed placental positivity for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
    Design.—: Case-based retrospective clinicopathologic analysis by a multinational group of 44 perinatal specialists from 12 countries of placental and autopsy pathology findings from 64 stillborns and 4 neonatal deaths having placentas testing positive for SARS-CoV-2 following delivery to mothers with COVID-19.
    Results.—: Of the 3 findings constituting SARS-CoV-2 placentitis, all 68 placentas had increased fibrin deposition and villous trophoblast necrosis and 66 had chronic histiocytic intervillositis. Sixty-three placentas had massive perivillous fibrin deposition. Severe destructive placental disease from SARS-CoV-2 placentitis averaged 77.7% tissue involvement. Other findings included multiple intervillous thrombi (37%; 25 of 68) and chronic villitis (32%; 22 of 68). The majority (19; 63%) of the 30 autopsies revealed no significant fetal abnormalities except for intrauterine hypoxia and asphyxia. Among all 68 cases, SARS-CoV-2 was detected from a body specimen in 16 of 28 cases tested, most frequently from nasopharyngeal swabs. Four autopsied stillborns had SARS-CoV-2 identified in internal organs.
    Conclusions.—: The pathology abnormalities composing SARS-CoV-2 placentitis cause widespread and severe placental destruction resulting in placental malperfusion and insufficiency. In these cases, intrauterine and perinatal death likely results directly from placental insufficiency and fetal hypoxic-ischemic injury. There was no evidence that SARS-CoV-2 involvement of the fetus had a role in causing these deaths.
    MeSH term(s) COVID-19/complications ; Female ; Fibrin ; Humans ; Hypoxia/pathology ; Hypoxia/virology ; Infant, Newborn ; Infectious Disease Transmission, Vertical ; Perinatal Death/etiology ; Placenta/pathology ; Pregnancy ; Pregnancy Complications, Infectious/mortality ; Pregnancy Complications, Infectious/pathology ; Pregnancy Complications, Infectious/virology ; Retrospective Studies ; SARS-CoV-2 ; Stillbirth
    Chemical Substances Fibrin (9001-31-4)
    Language English
    Publishing date 2022-02-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 194119-7
    ISSN 1543-2165 ; 0363-0153 ; 0096-8528 ; 0003-9985
    ISSN (online) 1543-2165
    ISSN 0363-0153 ; 0096-8528 ; 0003-9985
    DOI 10.5858/arpa.2022-0029-SA
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  10. Article ; Online: The pulmonary vasculature in lethal COVID-19 and idiopathic pulmonary fibrosis at single-cell resolution.

    de Rooij, Laura P M H / Becker, Lisa M / Teuwen, Laure-Anne / Boeckx, Bram / Jansen, Sander / Feys, Simon / Verleden, Stijn / Liesenborghs, Laurens / Stalder, Anna K / Libbrecht, Sasha / Van Buyten, Tina / Philips, Gino / Subramanian, Abhishek / Dumas, Sébastien J / Meta, Elda / Borri, Mila / Sokol, Liliana / Dendooven, Amélie / Truong, Anh-Co K /
    Gunst, Jan / Van Mol, Pierre / Haslbauer, Jasmin D / Rohlenova, Katerina / Menter, Thomas / Boudewijns, Robbert / Geldhof, Vincent / Vinckier, Stefan / Amersfoort, Jacob / Wuyts, Wim / Van Raemdonck, Dirk / Jacobs, Werner / Ceulemans, Laurens J / Weynand, Birgit / Thienpont, Bernard / Lammens, Martin / Kuehnel, Mark / Eelen, Guy / Dewerchin, Mieke / Schoonjans, Luc / Jonigk, Danny / van Dorpe, Jo / Tzankov, Alexandar / Wauters, Els / Mazzone, Massimiliano / Neyts, Johan / Wauters, Joost / Lambrechts, Diether / Carmeliet, Peter

    Cardiovascular research

    2022  Volume 119, Issue 2, Page(s) 520–535

    Abstract: Aims: Severe acute respiratory syndrome coronavirus-2 infection causes COVID-19, which in severe cases evokes life-threatening acute respiratory distress syndrome (ARDS). Transcriptome signatures and the functional relevance of non-vascular cell types ( ... ...

    Abstract Aims: Severe acute respiratory syndrome coronavirus-2 infection causes COVID-19, which in severe cases evokes life-threatening acute respiratory distress syndrome (ARDS). Transcriptome signatures and the functional relevance of non-vascular cell types (e.g. immune and epithelial cells) in COVID-19 are becoming increasingly evident. However, despite its known contribution to vascular inflammation, recruitment/invasion of immune cells, vascular leakage, and perturbed haemostasis in the lungs of severe COVID-19 patients, an in-depth interrogation of the endothelial cell (EC) compartment in lethal COVID-19 is lacking. Moreover, progressive fibrotic lung disease represents one of the complications of COVID-19 pneumonia and ARDS. Analogous features between idiopathic pulmonary fibrosis (IPF) and COVID-19 suggest partial similarities in their pathophysiology, yet, a head-to-head comparison of pulmonary cell transcriptomes between both conditions has not been implemented to date.
    Methods and results: We performed single-nucleus RNA-sequencing on frozen lungs from 7 deceased COVID-19 patients, 6 IPF explant lungs, and 12 controls. The vascular fraction, comprising 38 794 nuclei, could be subclustered into 14 distinct EC subtypes. Non-vascular cell types, comprising 137 746 nuclei, were subclustered and used for EC-interactome analyses. Pulmonary ECs of deceased COVID-19 patients showed an enrichment of genes involved in cellular stress, as well as signatures suggestive of dampened immunomodulation and impaired vessel wall integrity. In addition, increased abundance of a population of systemic capillary and venous ECs was identified in COVID-19 and IPF. COVID-19 systemic ECs closely resembled their IPF counterparts, and a set of 30 genes was found congruently enriched in systemic ECs across studies. Receptor-ligand interaction analysis of ECs with non-vascular cell types in the pulmonary micro-environment revealed numerous previously unknown interactions specifically enriched/depleted in COVID-19 and/or IPF.
    Conclusions: This study uncovered novel insights into the abundance, expression patterns, and interactomes of EC subtypes in COVID-19 and IPF, relevant for future investigations into the progression and treatment of both lethal conditions.
    MeSH term(s) Humans ; COVID-19 ; Idiopathic Pulmonary Fibrosis/genetics ; Idiopathic Pulmonary Fibrosis/metabolism ; Lung/metabolism ; Respiratory Distress Syndrome/metabolism ; Transcriptome
    Language English
    Publishing date 2022-09-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80340-6
    ISSN 1755-3245 ; 0008-6363
    ISSN (online) 1755-3245
    ISSN 0008-6363
    DOI 10.1093/cvr/cvac139
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