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  1. Article ; Online: Direct Measurement of the Branching Fractions B(ψ(3686)→J/ψX) and B(ψ(3770)→J/ψX), and Observation of the State R(3760) in e^{+}e^{-}→J/ψX.

    Ablikim, M / Achasov, M N / Adlarson, P / Ahmed, S / Albrecht, M / Amoroso, A / An, Q / Anita / Bai, Y / Bakina, O / Baldini Ferroli, R / Balossino, I / Ban, Y / Begzsuren, K / Bennett, J V / Berger, N / Bertani, M / Bettoni, D / Bianchi, F /
    Biernat, J / Bloms, J / Bortone, A / Boyko, I / Briere, R A / Cai, H / Cai, X / Calcaterra, A / Cao, G F / Cao, N / Cetin, S A / Chang, J F / Chang, W L / Chelkov, G / Chen, D Y / Chen, G / Chen, H S / Chen, M L / Chen, S J / Chen, X R / Chen, Y B / Cheng, W / Cibinetto, G / Cossio, F / Cui, X F / Dai, H L / Dai, J P / Dai, X C / Dbeyssi, A / de Boer, R B / Dedovich, D / Deng, Z Y / Denig, A / Denysenko, I / Destefanis, M / De Mori, F / Ding, Y / Dong, C / Dong, J / Dong, L Y / Dong, M Y / Du, S X / Fang, J / Fang, S S / Fang, Y / Farinelli, R / Fava, L / Feldbauer, F / Felici, G / Feng, C Q / Fritsch, M / Fu, C D / Fu, Y / Gao, X L / Gao, Y / Gao, Y G / Garzia, I / Gersabeck, E M / Gilman, A / Goetzen, K / Gong, L / Gong, W X / Gradl, W / Greco, M / Gu, L M / Gu, M H / Gu, S / Gu, Y T / Guan, C Y / Guo, A Q / Guo, L B / Guo, R P / Guo, Y P / Guskov, A / Han, S / Han, T T / Han, T Z / Hao, X Q / Harris, F A / He, K L / Heinsius, F H / Held, T / Heng, Y K / Himmelreich, M / Holtmann, T / Hou, Y R / Hou, Z L / Hu, H M / Hu, J F / Hu, T / Hu, Y / Huang, G S / Huang, L Q / Huang, X T / Huang, Z / Huesken, N / Hussain, T / Ikegami Andersson, W / Imoehl, W / Irshad, M / Jaeger, S / Janchiv, S / Ji, Q / Ji, Q P / Ji, X B / Ji, X L / Jiang, H B / Jiang, X S / Jiang, X Y / Jiao, J B / Jiao, Z / Jin, S / Jin, Y / Johansson, T / Kalantar-Nayestanaki, N / Kang, X S / Kappert, R / Kavatsyuk, M / Ke, B C / Keshk, I K / Khoukaz, A / Kiese, P / Kiuchi, R / Kliemt, R / Koch, L / Kolcu, O B / Kopf, B / Kuemmel, M / Kuessner, M / Kupsc, A / Kurth, M G / Kühn, W / Lane, J J / Lange, J S / Larin, P / Lavezzi, L / Leithoff, H / Lellmann, M / Lenz, T / Li, C / Li, C H / Li, Cheng / Li, D M / Li, F / Li, G / Li, H B / Li, H J / Li, J L / Li, J Q / Li, Ke / Li, L K / Li, Lei / Li, P L / Li, P R / Li, S Y / Li, W D / Li, W G / Li, X H / Li, X L / Li, Z B / Li, Z Y / Liang, H / Liang, Y F / Liang, Y T / Liao, L Z / Libby, J / Lin, C X / Liu, B / Liu, B J / Liu, C X / Liu, D / Liu, D Y / Liu, F H / Liu, Fang / Liu, Feng / Liu, H B / Liu, H M / Liu, Huanhuan / Liu, Huihui / Liu, J B / Liu, J Y / Liu, K / Liu, K Y / Liu, Ke / Liu, L / Liu, L Y / Liu, Q / Liu, S B / Liu, T / Liu, X / Liu, Y B / Liu, Z A / Liu, Z Q / Long, Y F / Lou, X C / Lu, H J / Lu, J D / Lu, J G / Lu, X L / Lu, Y / Lu, Y P / Luo, C L / Luo, M X / Luo, P W / Luo, T / Luo, X L / Lusso, S / Lyu, X R / Ma, F C / Ma, H L / Ma, L L / Ma, M M / Ma, Q M / Ma, R Q / Ma, R T / Ma, X N / Ma, X X / Ma, X Y / Ma, Y M / Maas, F E / Maggiora, M / Maldaner, S / Malde, S / Malik, Q A / Mangoni, A / Mao, Y J / Mao, Z P / Marcello, S / Meng, Z X / Messchendorp, J G / Mezzadri, G / Min, T J / Mitchell, R E / Mo, X H / Mo, Y J / Muchnoi, N Yu / Muramatsu, H / Nakhoul, S / Nefedov, Y / Nerling, F / Nikolaev, I B / Ning, Z / Nisar, S / Olsen, S L / Ouyang, Q / Pacetti, S / Pan, Y / Papenbrock, M / Pathak, A / Patteri, P / Pelizaeus, M / Peng, H P / Peters, K / Pettersson, J / Ping, J L / Ping, R G / Pitka, A / Poling, R / Prasad, V / Qi, H / Qi, H R / Qi, M / Qi, T Y / Qian, S / Qian, W-B / Qiao, C F / Qin, L Q / Qin, X P / Qin, X S / Qin, Z H / Qiu, J F / Qu, S Q / Rashid, K H / Ravindran, K / Redmer, C F / Rivetti, A / Rodin, V / Rolo, M / Rong, G / Rosner, Ch / Rump, M / Sarantsev, A / Savrié, M / Schelhaas, Y / Schnier, C / Schoenning, K / Shan, W / Shan, X Y / Shao, M / Shen, C P / Shen, P X / Shen, X Y / Shi, H C / Shi, R S / Shi, X / Shi, X D / Song, J J / Song, Q Q / Song, Y X / Sosio, S / Spataro, S / Sui, F F / Sun, G X / Sun, J F / Sun, L / Sun, S S / Sun, T / Sun, W Y / Sun, Y J / Sun, Y K / Sun, Y Z / Sun, Z T / Tan, Y X / Tang, C J / Tang, G Y / Tang, J / Thoren, V / Tsednee, B / Uman, I / Wang, B / Wang, B L / Wang, C W / Wang, D Y / Wang, H P / Wang, K / Wang, L L / Wang, M / Wang, M Z / Wang, Meng / Wang, W P / Wang, X / Wang, X F / Wang, X L / Wang, Y / Wang, Y D / Wang, Y F / Wang, Y Q / Wang, Z / Wang, Z Y / Wang, Ziyi / Wang, Zongyuan / Weber, T / Wei, D H / Weidenkaff, P / Weidner, F / Wen, H W / Wen, S P / White, D J / Wiedner, U / Wilkinson, G / Wolke, M / Wollenberg, L / Wu, J F / Wu, L H / Wu, L J / Wu, X / Wu, Z / Xia, L / Xiao, H / Xiao, S Y / Xiao, Y J / Xiao, Z J / Xie, X H / Xie, Y G / Xie, Y H / Xing, T Y / Xiong, X A / Xu, G F / Xu, J J / Xu, Q J / Xu, W / Xu, X P / Yan, L / Yan, W B / Yan, W C / Yang, H J / Yang, H X / Yang, L / Yang, R X / Yang, S L / Yang, Y H / Yang, Y X / Yang, Yifan / Yang, Zhi / Ye, M / Ye, M H / Yin, J H / You, Z Y / Yu, B X / Yu, C X / Yu, G / Yu, J S / Yu, T / Yuan, C Z / Yuan, W / Yuan, X Q / Yuan, Y / Yue, C X / Yuncu, A / Zafar, A A / Zeng, Y / Zhang, B X / Zhang, Guangyi / Zhang, H H / Zhang, H Y / Zhang, J L / Zhang, J Q / Zhang, J W / Zhang, J Y / Zhang, J Z / Zhang, Jianyu / Zhang, Jiawei / Zhang, L / Zhang, Lei / Zhang, S / Zhang, S F / Zhang, T J / Zhang, X Y / Zhang, Y / Zhang, Y H / Zhang, Y T / Zhang, Yan / Zhang, Yao / Zhang, Yi / Zhang, Z H / Zhang, Z Y / Zhao, G / Zhao, J / Zhao, J Y / Zhao, J Z / Zhao, Lei / Zhao, Ling / Zhao, M G / Zhao, Q / Zhao, S J / Zhao, Y B / Zhao Zhao, Y X / Zhao, Z G / Zhemchugov, A / Zheng, B / Zheng, J P / Zheng, Y / Zheng, Y H / Zhong, B / Zhong, C / Zhou, L P / Zhou, Q / Zhou, X / Zhou, X K / Zhou, X R / Zhu, A N / Zhu, J / Zhu, K / Zhu, K J / Zhu, S H / Zhu, W J / Zhu, X L / Zhu, Y C / Zhu, Z A / Zou, B S / Zou, J H

    Physical review letters

    2021  Volume 127, Issue 8, Page(s) 82002

    Abstract: ... collider. In analysis of the cross sections, we measured the decay branching fractions of B(ψ(3686)→J/ψX ... 64.4±0.6±1.6)% and B(ψ(3770)→J/ψX)=(0.5±0.2±0.1)% for the first time. The energy-dependent line shape ... branching fraction Γ_{R(3760)}^{ee}B[R(3760)→J/ψX]=(79.4±85.5±11.7)  eV. The significance of the R(3760) is ...

    Abstract We report a measurement of the observed cross sections of e^{+}e^{-}→J/ψX based on 3.21  fb^{-1} of data accumulated at energies from 3.645 to 3.891 GeV with the BESIII detector operated at the BEPCII collider. In analysis of the cross sections, we measured the decay branching fractions of B(ψ(3686)→J/ψX)=(64.4±0.6±1.6)% and B(ψ(3770)→J/ψX)=(0.5±0.2±0.1)% for the first time. The energy-dependent line shape of these cross sections cannot be well described by two Breit-Wigner (BW) amplitudes of the expected decays ψ(3686)→J/ψX and ψ(3770)→J/ψX. Instead, it can be better described with one more BW amplitude of the decay R(3760)→J/ψX. Under this assumption, we extracted the R(3760) mass M_{R(3760)}=3766.2±3.8±0.4  MeV/c^{2} , total width Γ_{R(3760)}^{tot}=22.2±5.9±1.4  MeV, and product of leptonic width and decay branching fraction Γ_{R(3760)}^{ee}B[R(3760)→J/ψX]=(79.4±85.5±11.7)  eV. The significance of the R(3760) is 5.3σ. The first uncertainties of these measured quantities are from fits to the cross sections and second systematic.
    Language English
    Publishing date 2021-09-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.127.082002
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  2. Article ; Online: scBCR-seq revealed a special and novel IG H&L V(D)J allelic inclusion rearrangement and the high proportion dual BCR expressing B cells.

    Zhu, Lanwei / Peng, Qi / Wu, Yingjie / Yao, Xinsheng

    Cellular and molecular life sciences : CMLS

    2023  Volume 80, Issue 11, Page(s) 319

    Abstract: Since the initial report of V (D) J "allelic exclusion/inclusion" (allelic exclusion rearrangement ... or allelic inclusion rearrangement) and the concept of the "dual B cell receptor (BCR)" in 1961 ... conditions have been puzzling immuologists. This study takes advantage of the single cell B cell receptor ...

    Abstract Since the initial report of V (D) J "allelic exclusion/inclusion" (allelic exclusion rearrangement or allelic inclusion rearrangement) and the concept of the "dual B cell receptor (BCR)" in 1961, despite ongoing discoveries, the precise proportion and source mechanism of dual BCR under physiological conditions have been puzzling immuologists. This study takes advantage of the single cell B cell receptor sequencing (scBCR-seq) technology, which can perfectly match the heavy and light chains of BCR at the level of a single B cell, and obtain the full length mRNA sequence of the complementary determining region 3 (CDR3). Through analyzing the pairing of functional IGH (immunoglobulin heavy chain) and IGL (immunoglobulin light chain) in single B cell from both human and mouse bone marrow and peripheral blood, it was observed that dual BCR B cells exhibit stable and high levels of expression. Among them, the human bone marrow and peripheral blood contain about 10% dual (or multiple) BCR B cells, while in mouse peripheral blood and bone marrow memory B cells, this proportion reaches around 20%. At the same time, we innovatively found that in each research sample of humans and mice, there are three (or more) functional rearrangements (mRNA level) of a single chain in a single B cell. By analyzing the position, direction and other compositional characteristics of the V(D)J gene family, we found that at least two (or more) of them are derived from over two (or more) specific allelic inclusion rearrangements of a single chromosome (mRNA molecular level evidence), our findings also highlighted the necessity of classified single cell sequencing data based on single, dual (or multiple) and cannot be assembled into BCR when analyzing the B cell repertoire. The results of this article provides new methods and modeling references for evaluating the proportion and source mechanisms of dual BCR B cells, as well as potential significance of allelic inclusion (exclusion escape) of V(D)J rearrangement.
    MeSH term(s) Mice ; Humans ; Animals ; Receptors, Antigen, B-Cell/genetics ; Receptors, Antigen, B-Cell/metabolism ; B-Lymphocytes/metabolism ; Immunoglobulin Heavy Chains/genetics ; Immunoglobulin Heavy Chains/metabolism ; RNA, Messenger/genetics
    Chemical Substances Receptors, Antigen, B-Cell ; Immunoglobulin Heavy Chains ; RNA, Messenger
    Language English
    Publishing date 2023-10-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-023-04973-8
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  3. Article ; Online: First observation of B(s)(0) → J/ψη and B(s)(0) → J/ψη'.

    Li, J / Adachi, I / Aihara, H / Arinstein, K / Asner, D M / Aulchenko, V / Aushev, T / Bakich, A M / Bhardwaj, V / Bhuyan, B / Bischofberger, M / Bondar, A / Bozek, A / Bračko, M / Brovchenko, O / Browder, T E / Chang, M-C / Chen, A / Chen, P /
    Cheon, B G / Chistov, R / Cho, K / Choi, S-K / Choi, Y / Dalseno, J / Doležal, Z / Drutskoy, A / Eidelman, S / Esen, S / Fast, J E / Gaur, V / Garmash, A / Goh, Y M / Haba, J / Hara, T / Hayasaka, K / Hayashii, H / Horii, Y / Hoshi, Y / Hou, W-S / Hsiung, Y B / Hyun, H J / Iijima, T / Inami, K / Ishikawa, A / Itoh, R / Iwabuchi, M / Iwasaki, Y / Iwashita, T / Julius, T / Kang, J H / Kapusta, P / Katayama, N / Kawasaki, T / Kim, H J / Kim, H O / Kim, J B / Kim, K T / Kim, M J / Kim, Y J / Kinoshita, K / Ko, B R / Kobayashi, N / Kodyš, P / Korpar, S / Križan, P / Krokovny, P / Kuhr, T / Kumar, R / Kuzmin, A / Kwon, Y-J / Lange, J S / Lee, M J / Lee, S-H / Li, Y / Libby, J / Liu, C / Liu, Y / Liu, Z Q / Liventsev, D / Louvot, R / Matvienko, D / McOnie, S / Miyazaki, Y / Mizuk, R / Mohanty, G B / Moll, A / Mori, T / Muramatsu, N / Nakamura, I / Nakano, E / Nakao, M / Nakazawa, H / Natkaniec, Z / Nishida, S / Nishimura, K / Nitoh, O / Ogawa, S / Ohshima, T / Okuno, S / Olsen, S L / Ostrowicz, W / Pakhlova, G / Park, C W / Park, H K / Park, K S / Pedlar, T K / Peng, T / Pestotnik, R / Petrič, M / Piilonen, L E / Prim, M / Röhrken, M / Ryu, S / Sahoo, H / Sakai, K / Sakai, Y / Sanuki, T / Sato, Y / Schneider, O / Schwanda, C / Schwartz, A J / Senyo, K / Seon, O / Sevior, M E / Shapkin, M / Shebalin, V / Shen, C P / Shibata, T-A / Shiu, J-G / Simon, F / Smerkol, P / Sohn, Y-S / Sokolov, A / Stanič, S / Starič, M / Sumihama, M / Sumiyoshi, T / Tanaka, S / Tatishvili, G / Teramoto, Y / Trabelsi, K / Uchida, M / Uehara, S / Unno, Y / Uno, S / Urquijo, P / Usov, Y / Varner, G / Varvell, K E / Vorobyev, V / Vossen, A / Wang, C H / Wang, P / Watanabe, M / Watanabe, Y / Wicht, J / Williams, K M / Won, E / Yamashita, Y / Yuan, C Z / Zhang, Z P / Zhilich, V / Zupanc, A

    Physical review letters

    2012  Volume 108, Issue 18, Page(s) 181808

    Abstract: We report first observations of B(s)(0) → J/ψη and B(s)(0) → J/ψη'. The results are obtained ... collider. We obtain the branching fractions B(B(s)(0) → J/ψη)=[5.10±0.50(stat)±0.25(syst)(-0.79)(+1.14)(N(B ... s)(*) B(s)(*))]×10(-4), and B(B(s)(0) → J/ψη')=[3.71±0.61(stat)±0.18(syst)(-0.57)(+0.83)(N(B(s)(*) B ...

    Abstract We report first observations of B(s)(0) → J/ψη and B(s)(0) → J/ψη'. The results are obtained from 121.4  fb(-1) of data collected at the Υ(5S) resonance with the Belle detector at the KEKB e+ e- collider. We obtain the branching fractions B(B(s)(0) → J/ψη)=[5.10±0.50(stat)±0.25(syst)(-0.79)(+1.14)(N(B(s)(*) B(s)(*))]×10(-4), and B(B(s)(0) → J/ψη')=[3.71±0.61(stat)±0.18(syst)(-0.57)(+0.83)(N(B(s)(*) B(s)(*))]×10(-4). The ratio of the two branching fractions is measured to be (B(B(s) → J/ψη'))/(B(B(s) → J/ψη))=0.73±0.14(stat)±0.02(syst).
    MeSH term(s) Elementary Particles ; Quantum Theory
    Language English
    Publishing date 2012-05-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.108.181808
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: [Research progress of common respiratory virus receptor binding sites].

    Peng, H Z / Li, J / Li, Y H / Xu, Y Q / Peng, J Q / Xie, X B

    Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine

    2024  Volume 57, Issue 12, Page(s) 2212–2219

    Abstract: Respiratory viral infections are an important public health problem worldwide, with complex mechanisms of infection, and the key to infection lies in the specific binding between respiratory viruses and receptors. This article provides an overview of the ...

    Abstract Respiratory viral infections are an important public health problem worldwide, with complex mechanisms of infection, and the key to infection lies in the specific binding between respiratory viruses and receptors. This article provides an overview of the progress in the study of receptors for respiratory viruses, such as coronavirus and influenza virus (IV), with a focus on the binding sites of receptors such as angiotensin-converting enzyme 2 (ACE2) and sialic acid (SA) to respiratory viruses and the role of receptor diversity in respiratory viral infections. An in-depth study of the binding sites between viruses and receptors will help to understand the molecular mechanism of respiratory viral infections and provide a theoretical basis for disease prevention and control and the development of new therapeutic targets.
    MeSH term(s) Humans ; Binding Sites ; Coronavirus Infections ; N-Acetylneuraminic Acid ; Viruses ; Receptors, Virus
    Chemical Substances N-Acetylneuraminic Acid (GZP2782OP0) ; Receptors, Virus
    Language Chinese
    Publishing date 2024-01-08
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 604575-3
    ISSN 0253-9624
    ISSN 0253-9624
    DOI 10.3760/cma.j.cn112150-20230911-00175
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  5. Article: [Analysis of clinical characteristics of persistent HBeAg positivity in patients with chronic hepatitis B treated with nucleos(t)ide analogues].

    Peng, L P / Gan, W Q / Zheng, Y B / Chen, Y M / Liu, J / Wu, Z B / Gao, Z L

    Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

    2024  Volume 32, Issue 3, Page(s) 193–200

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Female ; Humans ; Hepatitis B, Chronic/drug therapy ; Hepatitis B e Antigens ; Antiviral Agents/therapeutic use ; Hepatitis B Surface Antigens ; Retrospective Studies ; DNA, Viral ; Neoplasm Recurrence, Local/drug therapy ; Hepatitis B/drug therapy ; Liver Cirrhosis/drug therapy ; Hepatitis B virus/genetics ; Treatment Outcome
    Chemical Substances Hepatitis B e Antigens ; Antiviral Agents ; Hepatitis B Surface Antigens ; DNA, Viral
    Language Chinese
    Publishing date 2024-01-23
    Publishing country China
    Document type English Abstract ; Journal Article
    ISSN 1007-3418
    ISSN 1007-3418
    DOI 10.3760/cma.j.cn501113-20230822-00065
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  6. Article: [Current status and considerations on clinical application of function-preserving pancreatic surgery].

    Li, D / Qin, K / Jin, J B / Peng, C H

    Zhonghua wai ke za zhi [Chinese journal of surgery

    2024  Volume 62, Issue 4, Page(s) 338–345

    Abstract: For pancreatic neoplasms, the current clinical treatment strategy is mainly using standard surgical methods, including pancreaticoduodenectomy, distal pancreatectomy with splenectomy, and total pancreatectomy. Standard surgical methods require a larger ... ...

    Abstract For pancreatic neoplasms, the current clinical treatment strategy is mainly using standard surgical methods, including pancreaticoduodenectomy, distal pancreatectomy with splenectomy, and total pancreatectomy. Standard surgical methods require a larger resection, including resection of some surrounding organs and a large amount of pancreatic parenchyma. The endocrine and exocrine functions of the pancreas are easily damaged. Moreover, since the standard surgical procedure involves the reconstruction of the digestive tract at multiple anastomoses, there is a high risk of pancreatic, biliary, and intestinal fistulas occurring postoperatively. Therefore, function-preserving pancreatic surgery is recommended for some benign and low-grade pancreatic neoplasms. This type of surgery can treat pancreatic diseases while preserving more peripancreatic organs, pancreatic parenchyma and relatively complete digestive tract continuity, thereby improving the patient's short-term and long-term quality of life. In addition, with the development of laparoscopy and da Vinci robotic technology, minimally invasive technology-assisted pancreatic surgery has been carried out in clinical practice. They have been shown to be sufficiently safe and effective. This article reviews several common clinical pancreatic function-preserving surgical methods and their corresponding clinical applications and technical development status from the perspectives of preserving more peripancreatic organs, preserving more pancreatic parenchyma, and promoting pancreatic function recovery.
    MeSH term(s) Humans ; Quality of Life ; Pancreas/surgery ; Pancreatectomy/methods ; Pancreaticoduodenectomy/methods ; Pancreatic Neoplasms/surgery ; Laparoscopy
    Language Chinese
    Publishing date 2024-03-04
    Publishing country China
    Document type Review ; English Abstract ; Journal Article
    ZDB-ID 604573-x
    ISSN 0529-5815
    ISSN 0529-5815
    DOI 10.3760/cma.j.cn112139-20231116-00225
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  7. Article ; Online: Co-delivery of Cas9 mRNA and guide RNAs for editing of LGMN gene represses breast cancer cell metastasis.

    Wang, Yue / Peng, Yatu / Zi, Guanghui / Chen, Jin / Peng, Baowei

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 8095

    Abstract: Legumain (or asparagine endopeptidase/AEP) is a lysosomal cysteine endopeptidase associated with increased invasive and migratory behavior in a variety of cancers. In this study, co-delivery of Cas9 mRNA and guide RNA (gRNA) by lipid nanoparticles (LNP) ... ...

    Abstract Legumain (or asparagine endopeptidase/AEP) is a lysosomal cysteine endopeptidase associated with increased invasive and migratory behavior in a variety of cancers. In this study, co-delivery of Cas9 mRNA and guide RNA (gRNA) by lipid nanoparticles (LNP) for editing of LGMN gene was performed. For in-vitro transcription (IVT) of gRNA, two templates were designed: linearized pUC57-T7-gRNA and T7-gRNA oligos, and the effectiveness of gRNA was verified in multiple ways. Cas9 plasmid was modified and optimized for IVT of Cas9 mRNA. The effects of LGMN gene editing on lysosomal/autophagic function and cancer cell metastasis were investigated. Co-delivery of Cas9 mRNA and gRNA resulted in impaired lysosomal/autophagic degradation, clone formation, migration, and invasion capacity of cancer cells in-vitro. Experimental lung metastasis experiment indicates co-delivery of Cas9 mRNA and gRNA by LNP reduced the migration and invasion capacity of cancer cells in-vivo. These results indicate that co-delivery of Cas9 mRNA and gRNA can enhance the efficiency of CRISPR/Cas9-mediated gene editing in-vitro and in-vivo, and suggest that Cas9 mRNA and gRNA gene editing of LGMN may be a potential treatment for breast tumor metastasis.
    MeSH term(s) Humans ; Female ; CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Breast Neoplasms/genetics ; Gene Editing/methods
    Chemical Substances RNA, Guide, CRISPR-Cas Systems ; RNA, Messenger
    Language English
    Publishing date 2024-04-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-58765-6
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  8. Article ; Online: Hyperthermia and cisplatin combination therapy promotes caspase-8 accumulation and activation to enhance apoptosis and pyroptosis in cancer cells.

    Zi, Guanghui / Chen, Jin / Peng, Yatu / Wang, Yue / Peng, Baowei

    International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group

    2024  Volume 41, Issue 1, Page(s) 2325489

    Abstract: Background: Hyperthermia can play a synergistic role with chemotherapy in combination therapy. Although the association between caspase activation, apoptosis, and pyroptosis have been published for both cisplatin (CDDP) and hyperthermia therapies ... ...

    Abstract Background: Hyperthermia can play a synergistic role with chemotherapy in combination therapy. Although the association between caspase activation, apoptosis, and pyroptosis have been published for both cisplatin (CDDP) and hyperthermia therapies independently, the interactions between these molecular pathways in combination therapy are unknown. The present study aimed to investigate the possible interactions between caspase 8 activation, apoptosis, and pyroptosis in combination therapy.
    Methods: Cells were treated with CDDP (15 µg/ml), followed by hyperthermia at optimized temperature (42.5 °C) in water-bath. After combination therapy, cell viability was analyzed by CCK-8, and cell death was analyzed by Annexin-V-FITC/PI and caspases activation. Immuno-staining and co-immuno-precipitation were used to examine the interaction between p62 and caspase-8. Pyroptosis was investigated by western blotting and transmission electron microscopy. E3 ligase Cullin 3 was knockdown by siRNA. In addition, caspase-8 activation was modulated by CRISPR-Cas9 gene-editing or pharmacological inhibition.
    Results: Combination therapy promoted K63-linked polyubiquitination of caspase-8 and cellular accumulation of caspase-8. In turn, polyubiquitinated caspase-8 interacted with p62 and led to the activation of caspase-3. Knockdown of the E3 ligase Cullin 3 by siRNA reduced caspase-8 polyubiquitination and activation. In addition, combination therapy induced release of the pore-forming N-terminus from gasdermins and promoted pyroptosis along with caspase-8 accumulation and activation. Knockdown of caspase-8 by CRISPR/Cas9 based gene editing reduced the sensitivity of tumor cells to apoptosis and pyroptosis.
    Conclusions: Our study presented a novel mechanism in which hyperthermia synergized with chemotherapy in promoting apoptosis and pyroptosis in a caspase-8 dependent manner.
    Language English
    Publishing date 2024-04-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 632526-9
    ISSN 1464-5157 ; 0265-6736
    ISSN (online) 1464-5157
    ISSN 0265-6736
    DOI 10.1080/02656736.2024.2325489
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: [Effect of HBV DNA load on the safety and prognosis of systematic therapy in advanced hepatocellular carcinoma].

    Zheng, X R / Peng, J X / Song, X / Liu, B / Zhong, C / Chen, X Y / Zhang, B X / Peng, L / Zhu, K S / Xie, C

    Zhonghua yi xue za zhi

    2024  Volume 104, Issue 14, Page(s) 1160–1167

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Humans ; Male ; Middle Aged ; Female ; Carcinoma, Hepatocellular/therapy ; DNA, Viral/analysis ; DNA, Viral/pharmacology ; DNA, Viral/therapeutic use ; Liver Neoplasms/therapy ; Retrospective Studies ; Hepatitis B ; Hepatitis B virus/genetics ; Prognosis ; Antiviral Agents/therapeutic use
    Chemical Substances DNA, Viral ; Antiviral Agents
    Language Chinese
    Publishing date 2024-04-07
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 132513-9
    ISSN 0376-2491
    ISSN 0376-2491
    DOI 10.3760/cma.j.cn112137-20231110-01055
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Editorial: Hallmark of cancer: avoiding immune suppression.

    Alenzi, Faris Q B / Apollonio, Benedetta / Peng, Liusheng / Sayour, Elias J / Sheffer, Michal

    Frontiers in oncology

    2023  Volume 13, Page(s) 1211456

    Language English
    Publishing date 2023-06-16
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2023.1211456
    Database MEDical Literature Analysis and Retrieval System OnLINE

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