LIVIVO - Search results -

Search results

Result 1 - 10 of total 32

Search options

Article ; Online: A Review of Therapeutic Antibodies in Breast Cancer.

Eini, Maryam / Zainodini, Nahid / Montazeri, Hamed / Mirzabeigi, Parastoo / Tarighi, Parastoo

Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques

2021 Volume 24, Page(s) 363–380

Abstract: Since the first production of monoclonal antibodies about 35 years ago, researchers have found them useful in the treatment and diagnosis of various diseases such as cancer. By developing different types of monoclonal antibodies such as humanized, drug ... ...

Abstract	Since the first production of monoclonal antibodies about 35 years ago, researchers have found them useful in the treatment and diagnosis of various diseases such as cancer. By developing different types of monoclonal antibodies such as humanized, drug conjugated, or bispecific ones, researchers, have achieved remarkable success in treating several complicated and challenging diseases, targeting specific antigens or receptors makes monoclonal antibodies the right choice to inhibit signaling pathways like programmed death-ligand 1 (PD-L1) or programmed death1 (PD-1) and changing cell behavior. As one of the most common types of malignancies among women, breast cancer is one of the most critical conditions which different types of monoclonal antibodies were designed and produced to treat. Therefore, we reviewed these antibodies in breast cancer, their targets, and their efficacy and toxicity, with more focus on recent PD-L1or PD-1 inhibitor antibodies in breast cancer and beyond.
MeSH term(s)	Animals ; Antibodies, Monoclonal/administration & dosage ; Antibodies, Monoclonal/adverse effects ; Antibodies, Monoclonal/pharmacology ; Antineoplastic Agents, Immunological/administration & dosage ; Antineoplastic Agents, Immunological/adverse effects ; Antineoplastic Agents, Immunological/pharmacology ; B7-H1 Antigen/immunology ; Breast Neoplasms/drug therapy ; Breast Neoplasms/immunology ; Female ; Humans ; Programmed Cell Death 1 Receptor/immunology
Chemical Substances	Antibodies, Monoclonal ; Antineoplastic Agents, Immunological ; B7-H1 Antigen ; CD274 protein, human ; PDCD1 protein, human ; Programmed Cell Death 1 Receptor
Language	English
Publishing date	2021-07-23
Publishing country	Canada
Document type	Journal Article ; Review
ZDB-ID	1422972-9
ISSN	1482-1826 ; 1482-1826
ISSN (online)	1482-1826
ISSN	1482-1826
DOI	10.18433/jpps31864
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Protective effect of selenium on vincristine-induced peripheral neuropathy in PC12 cell line.

Jafari, Davod / Eslami, Seyed Sadegh / Malih, Sara / Tarighi, Parastoo

Cytotechnology

2022 Volume 74, Issue 5, Page(s) 539–547

Abstract: Vincristine-induced peripheral neuropathy (VIPN) is the main side effect and major reason for neuropathic pain in cancer survivors treated with vincristine. Vincristine, a chemotherapeutic antimitotic drug, is used frequently in combination chemotherapy. ...

Abstract	Vincristine-induced peripheral neuropathy (VIPN) is the main side effect and major reason for neuropathic pain in cancer survivors treated with vincristine. Vincristine, a chemotherapeutic antimitotic drug, is used frequently in combination chemotherapy. The primary purpose of the current study was to assess the protective effect of sodium selenite (SSe) on VIPN in vitro. Cytotoxicity effects of vincristine were evaluated using PC12 cells as a neuronal model. The cell culture studies were conducted in three groups based on the various treatments, including vincristine, SSe, and co-exposure to both compositions. Cell viability and cell cycle analyses were performed using MTT assay and flow cytometry, respectively. The level of mRNA expression of
Language	English
Publishing date	2022-08-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	1035772-5
ISSN	0920-9069
ISSN	0920-9069
DOI	10.1007/s10616-022-00543-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2604: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Production and Conjugation of Truncated Recombinant Diphtheria Toxin to VEGFR-2 Specific Nanobody and Evaluation of its Cytotoxic Effect on PC-3 Cell Line.

Shajari, Samira / Farajollahi, Mohammad Morad / Behdani, Mahdi / Tarighi, Parastoo

Molecular biotechnology

2022 Volume 64, Issue 11, Page(s) 1218–1226

Abstract: Immunotoxins have represented a great potency in targeted therapeutics to encounter tumors. They consist of a protein toxin conjugated to a targeting moiety, which recognizes a specific antigen on surface of cancer cells and accordingly induces cell ... ...

Abstract	Immunotoxins have represented a great potency in targeted therapeutics to encounter tumors. They consist of a protein toxin conjugated to a targeting moiety, which recognizes a specific antigen on surface of cancer cells and accordingly induces cell death by toxin segment. The targeting part could be a nanobody, which is a group of antibodies composed of an only functional single variable heavy chain (VHH).Therefore, this study was done to produce an immunotoxin (VGRNb-DT) by chemical conjugation of a truncated diphtheria toxin moiety to an anti-vascular endothelial growth factor receptor 2(VEGFR-2) nanobody, and to identify effectiveness of immunotoxin in recognizing the VEGFR-2- positive cancer cells and inhibiting cell growth and survival. Diphtheria toxin was expressed and purified by nickel affinity chromatography, and accordingly, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis confirmed its expression. Function of heterobifunctional crosslinkers, Sulfo-SMCC (sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate), and SATP (N-succinimidyl-S- acetylthiopropionate) for bioconjugation purposes was acknowledged by cation exchange high-performance liquid chromatography (HPLC). Cytotoxicity of immunotoxin was evaluated on the VEGFR-2 positive PC-3 cell line by MTT assay. Overexpression of VEGFR-2 in the PC-3 cell line allowed immunotoxin to recognize them by anti-VEGFR-2 nanobodies. The concentrations above 5 μg/ml represented a significant decrease in cell survival rate in PC-3 cells compared to HEK293 cells (VEGFR-2 negative cells) as controls.VGRNb-DT demonstrated a successful bioconjugation; furthermore, variable concentrations were correlated with cell death in prostate cancer PC-3 cells.
MeSH term(s)	Antineoplastic Agents/pharmacology ; Cyclohexanes ; Diphtheria Toxin/genetics ; HEK293 Cells ; Humans ; Immunotoxins/genetics ; Immunotoxins/pharmacology ; Male ; Nickel ; PC-3 Cells ; Single-Domain Antibodies/genetics ; Single-Domain Antibodies/pharmacology ; Sodium Dodecyl Sulfate ; Vascular Endothelial Growth Factor Receptor-2/metabolism
Chemical Substances	Antineoplastic Agents ; Cyclohexanes ; Diphtheria Toxin ; Immunotoxins ; Single-Domain Antibodies ; Sodium Dodecyl Sulfate (368GB5141J) ; Nickel (7OV03QG267) ; KDR protein, human (EC 2.7.10.1) ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1)
Language	English
Publishing date	2022-04-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	1193057-3
ISSN	1559-0305 ; 1073-6085
ISSN (online)	1559-0305
ISSN	1073-6085
DOI	10.1007/s12033-022-00485-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 4031: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: A review of potential suggested drugs for coronavirus disease (COVID-19) treatment.

Tarighi, Parastoo / Eftekhari, Samane / Chizari, Milad / Sabernavaei, Mahsa / Jafari, Davod / Mirzabeigi, Parastoo

European journal of pharmacology

2021 Volume 895, Page(s) 173890

Abstract: The latest pandemic, coronavirus disease-2019 (COVID-19), is associated with high prevalence and easy transmission, which is expanding globally with no conventional treatment or vaccine. The new virus revealed 79% and 50% genomic similarities with severe ...

Abstract	The latest pandemic, coronavirus disease-2019 (COVID-19), is associated with high prevalence and easy transmission, which is expanding globally with no conventional treatment or vaccine. The new virus revealed 79% and 50% genomic similarities with severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively. Accordingly, since the disease resists testing and adopting new therapeutics, repositioning pre-existing drugs may present a fast and attractive strategy with known safety, characteristics, and dosage used. However, they are not specific and targeted. Therefore, several drugs have been investigated for their efficacy and safety in the treatment of COVID-19; most of them are undergoing clinical trials. This article summarizes clinical investigations of potential therapeutic drugs used as COVID-19 therapy. Subsequently, it prepares a pattern of results and therapeutic targets to help further experiment designs. We have investigated drugs as classified in the following three groups; 1) The drugs which computationally showed effectiveness (in silico) but needed further lab confirmations; 2) Emetine, Teicoplanin, and Nelfinavir have shown effectiveness in vitro; 3) The drugs currently under clinical trial.
MeSH term(s)	Animals ; Antiviral Agents/therapeutic use ; COVID-19/virology ; Humans ; Pandemics/prevention & control ; SARS-CoV-2/drug effects ; COVID-19 Drug Treatment
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2021-01-20
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	80121-5
ISSN	1879-0712 ; 0014-2999
ISSN (online)	1879-0712
ISSN	0014-2999
DOI	10.1016/j.ejphar.2021.173890
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 522: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Comparative evaluation of hydrogen peroxide sporicidal efficacy by different standard test methods.

Sadeghi, Simin / Abdollahi, Soosan / Tarighi, Parastoo / Samadi, Nasrin

Iranian journal of microbiology

2020 Volume 12, Issue 2, Page(s) 113–120

Abstract: Background and objectives: There are different sporicidal standard tests with various specifications to deal with products that are claimed for sporicidal activity. The aim of this study was to compare the 7% H: Materials and methods: The 7% H: ... ...

Abstract	Background and objectives: There are different sporicidal standard tests with various specifications to deal with products that are claimed for sporicidal activity. The aim of this study was to compare the 7% H Materials and methods: The 7% H Results: The results of suspension tests with 3 × 10 Conclusion: The results of this study showed that it is reasonable to use interfering substances and inoculated carriers in accordance with actual conditions of product usage in a sporicidal test. Interfering substances may reduce the contact surface between H
Language	English
Publishing date	2020-05-01
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2652849-6
ISSN	2008-4447 ; 2008-3289
ISSN (online)	2008-4447
ISSN	2008-3289
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Effects of Sitagliptin as Monotherapy and Add-On to Metformin on Weight Loss among Overweight and Obese Patients with Type 2 Diabetes: A Systematic Review and Meta-Analysis.

Janani, Leila / Bamehr, Hadi / Tanha, Kiarash / Mirzabeigi, Parastoo / Montazeri, Hamed / Tarighi, Parastoo

Drug research

2021 Volume 71, Issue 9, Page(s) 477–488

Abstract: Background: Sitagliptin is known as an antidiabetic agent inhibiting the dipeptidyl peptidase-4. Although sitagliptin may influence weight, controversial results have been reported, and there is no general agreement on this issue. Therefore, this study ... ...

Abstract	Background: Sitagliptin is known as an antidiabetic agent inhibiting the dipeptidyl peptidase-4. Although sitagliptin may influence weight, controversial results have been reported, and there is no general agreement on this issue. Therefore, this study assessed the effect of sitagliptin as monotherapy and add-on therapy to metformin on weight reduction in overweight or obese cases with type 2 diabetes. Methods: We reviewed the following databases to identify all relevant papers published until 1st April 2021: Web of Science, MEDLINE, Embase, Scopus, Cochrane Central Register of Controlled Trials Cochrane Library, and Google Scholar. The research included all clinical trials investigating the effect of sitagliptin in obese or overweight adult patients with type 2 diabetes without any language restriction. Results: In total, eighteen randomized controlled trials with 2009 participants were included in our meta-analysis. Results showed supplementation of sitagliptin has led to weight loss for sitagliptin treated (MD -0.99; 95% CI; (-1.87, -0.12); p=0.026)) and sitagliptin+metformin treated groups (MD -1.09; 95% CI; (-1.69, -0.49); p<0.001)). Also, the intervention has influenced body mass index in sitagliptin treated (MD -0.23; 95% CI; (-0.45, 0.02); p=0.033)) and sitagliptin+metformin treated groups (MD -0.52; 95% CI; (-0.96, 0.08); p=0.020)) comparing to placebo. Conclusion: Our results demonstrated that sitagliptin administration with or without metformin might reduce the body weight and body mass index if these drugs are taken for more than 6 months.
MeSH term(s)	Adult ; Diabetes Mellitus, Type 2/complications ; Diabetes Mellitus, Type 2/drug therapy ; Drug Therapy, Combination ; Glycated Hemoglobin A ; Humans ; Hypoglycemic Agents/therapeutic use ; Metformin/therapeutic use ; Obesity/complications ; Obesity/drug therapy ; Overweight/complications ; Overweight/drug therapy ; Randomized Controlled Trials as Topic ; Sitagliptin Phosphate/therapeutic use ; Weight Loss
Chemical Substances	Glycated Hemoglobin A ; Hypoglycemic Agents ; Metformin (9100L32L2N) ; Sitagliptin Phosphate (TS63EW8X6F)
Language	English
Publishing date	2021-08-13
Publishing country	Germany
Document type	Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	2703847-6
ISSN	2194-9387 ; 2194-9379
ISSN (online)	2194-9387
ISSN	2194-9379
DOI	10.1055/a-1555-2797
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uf I Zs.151: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Fabrication of a microfluidic device for probiotic drug's dosage screening: Precision Medicine for Breast Cancer Treatment.

Salehi, Ali / Naserzadeh, Parvaneh / Tarighi, Parastoo / Afjeh-Dana, Elham / Akhshik, Masoud / Jafari, Amir / Mackvandi, Pooyan / Ashtari, Behnaz / Mozafari, Masoud

Translational oncology

2023 Volume 34, Page(s) 101674

Abstract: Breast cancer is the most common cancer in women; it has been affecting the lives of millions each year globally and microfluidic devices seem to be a promising method for the future advancements in this field. This research uses a dynamic cell culture ... ...

Abstract	Breast cancer is the most common cancer in women; it has been affecting the lives of millions each year globally and microfluidic devices seem to be a promising method for the future advancements in this field. This research uses a dynamic cell culture condition in a microfluidic concentration gradient device, helping us to assess breast anticancer activities of probiotic strains against MCF-7 cells. It has been shown that MCF-7 cells could grow and proliferate for at least 24 h; however, a specific concentration of probiotic supernatant could induce more cell death signaling population after 48 h. One of our key findings was that our evaluated optimum dose (7.8 mg/L) was less than the conventional static cell culture treatment dose (12 mg/L). To determine the most effective dose over time and the percentage of apoptosis versus necrosis, flowcytometric assessment was performed. Exposing the MCF-7 cells to probiotic supernatant after 6, 24 and 48 h, confirmed that the apoptotic and necrotic cell death signaling were concentration and time dependent. We have shown a case that these types of microfluidics platforms performing dynamic cell culture could be beneficial in personalized medicine and cancer therapy.
Language	English
Publishing date	2023-05-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2443840-6
ISSN	1936-5233
ISSN	1936-5233
DOI	10.1016/j.tranon.2023.101674
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Rational peptide design for targeting cancer cell invasion.

Gomari, Mohammad Mahmoudi / Arab, Seyed Shahriar / Balalaie, Saeed / Ramezanpour, Sorour / Hosseini, Arshad / Dokholyan, Nikolay V / Tarighi, Parastoo

Proteins

2023 Volume 92, Issue 1, Page(s) 76–95

Abstract: Cell invasion is an important process in cancer progression and recurrence. Invasion and implantation of cancer cells from their original place to other tissues, by disabling vital organs, challenges the treatment of cancer patients. Given the importance ...

Abstract	Cell invasion is an important process in cancer progression and recurrence. Invasion and implantation of cancer cells from their original place to other tissues, by disabling vital organs, challenges the treatment of cancer patients. Given the importance of the matter, many molecular treatments have been developed to inhibit cancer cell invasion. Because of their low production cost and ease of production, peptides are valuable therapeutic molecules for inhibiting cancer cell invasion. In recent years, advances in the field of computational biology have facilitated the design of anti-cancer peptides. In our investigation, using computational biology approaches such as evolutionary analysis, residue scanning, protein-peptide interaction analysis, molecular dynamics, and free energy analysis, our team designed a peptide library with about 100 000 candidates based on A6 (acetyl-KPSSPPEE-amino) sequence which is an anti-invasion peptide. During computational studies, two of the designed peptides that give the highest scores and showed the greatest sequence similarity to A6 were entered into the experimental analysis workflow for further analysis. In experimental analysis steps, the anti-metastatic potency and other therapeutic effects of designed peptides were evaluated using MTT assay, RT-qPCR, zymography analysis, and invasion assay. Our study disclosed that the IK1 (acetyl-RPSFPPEE-amino) peptide, like A6, has great potency to inhibit the invasion of cancer cells.
MeSH term(s)	Humans ; Urokinase-Type Plasminogen Activator/chemistry ; Urokinase-Type Plasminogen Activator/pharmacology ; Urokinase-Type Plasminogen Activator/therapeutic use ; Receptors, Urokinase Plasminogen Activator ; Peptides/pharmacology ; Neoplasm Invasiveness
Chemical Substances	Urokinase-Type Plasminogen Activator (EC 3.4.21.73) ; Receptors, Urokinase Plasminogen Activator ; Peptides
Language	English
Publishing date	2023-08-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	806683-8
ISSN	1097-0134 ; 0887-3585
ISSN (online)	1097-0134
ISSN	0887-3585
DOI	10.1002/prot.26580
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2291: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Synergistic anti-tumor effects of Liraglutide, a glucagon-like peptide-1 receptor agonist, along with Docetaxel on LNCaP prostate cancer cell line.

Eftekhari, Samane / Montazeri, Hamed / Tarighi, Parastoo

European journal of pharmacology

2020 Volume 878, Page(s) 173102

Abstract: Docetaxel is a first line chemotherapy agent, which stabilizes microtubules in metastatic prostate cancer (PCa). Resistance to Docetaxel and its side effects remain as obstacle for its efficacy in monotherapy. Recently, combination with novel adjuvants ... ...

Abstract	Docetaxel is a first line chemotherapy agent, which stabilizes microtubules in metastatic prostate cancer (PCa). Resistance to Docetaxel and its side effects remain as obstacle for its efficacy in monotherapy. Recently, combination with novel adjuvants have been emerged as a beneficial alternative strategy, which targets multiple important pathways and also requires lower therapeutic dosage, proposing as a strategy to overcome drug resistance. This study investigated whether Liraglutide, a Glucagon Like Peptide-1 Receptor agonist, can reinforce the effect of Docetaxel on LNCaP prostate cancer cell line. Cells were treated by Liraglutide and Docetaxel, alone and in combination. Cytotoxicity was evaluated by MTT assay. Compusyn and Combenefit softwares were used in order to evaluate synergistic efficacy. Apoptosis was determined by Cell cycle analysis and Annexin-V/Propidium iodide staining through flow cytometry. However, the mRNA level of pro-apoptotic gene "Bax" and anti-apoptotic "Bcl-2" were evaluated by quantitative Real-Time PCR. Also, phosphorylation level of ERK1/2 and AKT proteins was investigated by western blotting technique. The results showed that Docetaxel and Liraglutide decreased the viability of LNCaP cells synergistically, caused cell cycle arrest and induced apoptosis potentially. The key proteins' evaluation in ERK/MAPK and AKT/PI3K pathways revealed a significant reduction in phosphorylation level of cells exposed to combination of drugs. Our results suggest that, the combination of Liraglutide and Docetaxel could be considered as a potent strategy in enhancing the efficacy of treatment, decreasing the Docetaxel therapeutic dose and thereby lowering systemic toxicities and resistances.
MeSH term(s)	Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Cell Line, Tumor ; Cell Survival/drug effects ; Docetaxel/pharmacology ; Dose-Response Relationship, Drug ; Drug Resistance, Neoplasm/drug effects ; Drug Synergism ; Drug Therapy, Combination/methods ; Gene Expression Regulation/drug effects ; Glucagon-Like Peptide-1 Receptor/agonists ; Humans ; Liraglutide/pharmacology ; MAP Kinase Signaling System ; Male ; Mitogen-Activated Protein Kinase 3/metabolism ; Prostatic Neoplasms/drug therapy ; Proto-Oncogene Proteins c-akt/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism ; RNA, Messenger/drug effects ; bcl-2-Associated X Protein/metabolism
Chemical Substances	Antineoplastic Agents ; BCL2 protein, human ; Glucagon-Like Peptide-1 Receptor ; Proto-Oncogene Proteins c-bcl-2 ; RNA, Messenger ; bcl-2-Associated X Protein ; Docetaxel (15H5577CQD) ; Liraglutide (839I73S42A) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; MAPK3 protein, human (EC 2.7.11.24) ; Mitogen-Activated Protein Kinase 3 (EC 2.7.11.24)
Language	English
Publishing date	2020-04-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80121-5
ISSN	1879-0712 ; 0014-2999
ISSN (online)	1879-0712
ISSN	0014-2999
DOI	10.1016/j.ejphar.2020.173102
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 522: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Paliurus spina-christi Mill fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells.

Esfahani, Seyedeh Mona Mousavi / Tarighi, Parastoo / Dianat, Kosar / Ashour, Tabarek Mahdi / Mottaghi-Dastjerdi, Negar / Aghsami, Mehdi / Sabernavaei, Mahsa / Montazeri, Hamed

BMC complementary medicine and therapies

2023 Volume 23, Issue 1, Page(s) 151

Abstract: Background: Paliurus spina-christi Mill. (PSC) fruit is frequently used in the treatment of diabetes mellitus in Mediterranean regions. Here, we investigated the effects of various PSC fruit extracts (PSC-FEs) on glucose consumption and some key ... ...

Abstract	Background: Paliurus spina-christi Mill. (PSC) fruit is frequently used in the treatment of diabetes mellitus in Mediterranean regions. Here, we investigated the effects of various PSC fruit extracts (PSC-FEs) on glucose consumption and some key mediators of insulin signaling pathways in high glucose and high insulin-induced insulin-resistant HepG2 cells. Methods: The effects of methanolic, chloroform and total extracts on cell proliferation were assessed by the MTT assay. The potential of non-toxic extracts on glucose utilization in insulin-resistant HepG2 cells was checked using a glucose oxidase assay. AKT and AMP-activated protein kinase (AMPK) pathway activation and mRNA expression levels of insulin receptor (INSR), glucose transporter 1 (GLUT1), and glucose transporters 4 (GLUT4) were determined by western blotting and real-time PCR, respectively. Results: We found that high concentrations of methanolic and both low and high concentrations of total extracts were able to enhance glucose uptake in an insulin-resistant cell line model. Moreover, AKT and AMPK phosphorylation were significantly increased by the high strength of methanolic extract, while total extract raised AMPK activation at low and high concentrations. Also, GLUT 1, GLUT 4, and INSR were elevated by both methanolic and total extracts. Conclusions: Ultimately, our results shed new light on methanolic and total PSC-FEs as sources of potential anti-diabetic medications, restoring glucose consumption and uptake in insulin-resistant HepG2 cells. These could be at least in part due to re-activating AKT and AMPK signaling pathways and also increased expression of INSR, GLUT1, and GLUT4. Overall, active constituents present in methanolic and total extracts of PCS are appropriate anti-diabetic agents and explain the use of these PSC fruits in traditional medicine for the treatment of diabetes.
MeSH term(s)	Hep G2 Cells ; Humans ; Rhamnaceae/chemistry ; Fruit/chemistry ; Insulin Resistance ; Signal Transduction/drug effects ; Glucose/metabolism ; Insulin/metabolism ; Plant Extracts/chemistry ; Cell Proliferation/drug effects ; Cell Survival/drug effects
Chemical Substances	Glucose (IY9XDZ35W2) ; Insulin ; Plant Extracts
Language	English
Publishing date	2023-05-08
Publishing country	England
Document type	Journal Article
ISSN	2662-7671
ISSN (online)	2662-7671
DOI	10.1186/s12906-023-03977-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED