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Article ; Online: Ammonia removal and simultaneous immobilization of manganese and magnesium from electrolytic manganese residue by a low-temperature CaO roasting process.

Huang, Lirong / Li, Xiaoqin / Li, Qingrui / Wang, Qian / Zhao, Feiping / Liu, Weizhen

Environmental science and pollution research international

2024 Volume 31, Issue 7, Page(s) 11321–11333

Abstract: A large amount of open-dumped electrolytic manganese residue (EMR) has posed a severe threat to the ecosystem and public health due to the leaching of ammonia ( ... ...

Abstract	A large amount of open-dumped electrolytic manganese residue (EMR) has posed a severe threat to the ecosystem and public health due to the leaching of ammonia (NH
MeSH term(s)	Manganese/chemistry ; Ammonia/analysis ; Magnesium ; Manganese Compounds/chemistry ; Calcium Sulfate ; Temperature ; Ecosystem ; Oxides/chemistry ; Electrolytes/chemistry
Chemical Substances	Manganese (42Z2K6ZL8P) ; Ammonia (7664-41-7) ; Magnesium (I38ZP9992A) ; Manganese Compounds ; Calcium Sulfate (WAT0DDB505) ; Oxides ; Electrolytes
Language	English
Publishing date	2024-01-13
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-024-31895-0
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Article ; Online: Structure-based neutralizing mechanisms for SARS-CoV-2 antibodies.

Huang, Qingrui / Han, Xiaonan / Yan, Jinghua

Emerging microbes & infections

2022 Volume 11, Issue 1, Page(s) 2412–2422

Abstract: The devastating economic and public health consequences caused by the COVID-19 pandemic have prompted outstanding efforts from the scientific community and pharmaceutical companies to develop antibody-based therapeutics against SARS-CoV-2. Those efforts ... ...

Abstract	The devastating economic and public health consequences caused by the COVID-19 pandemic have prompted outstanding efforts from the scientific community and pharmaceutical companies to develop antibody-based therapeutics against SARS-CoV-2. Those efforts are encouraging and fruitful. An unprecedentedly large number of monoclonal antibodies (mAbs) targeting a large spectrum of epitopes on the spike protein has been developed in the last two years. The development of structural biology, especially the cryo-EM technology, provides structural insights into the molecular neutralizing mechanisms of those mAbs. Moreover, neutralizing antibodies are essential in protecting host from infection. Therefore, understanding the antibody neutralizing mechanism is critical for optimizing effective antibody-based therapeutics and developing next-generation pan-coronavirus vaccines. This review summarizes the latest understanding of antibody neutralizing mechanisms against SARS-CoV-2 at the molecular and structural levels.
MeSH term(s)	Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; Epitopes ; Humans ; Pandemics/prevention & control ; Pharmaceutical Preparations ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Vaccines
Chemical Substances	Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral ; Epitopes ; Pharmaceutical Preparations ; Spike Glycoprotein, Coronavirus ; Vaccines ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-09-15
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2681359-2
ISSN	2222-1751 ; 2222-1751
ISSN (online)	2222-1751
ISSN	2222-1751
DOI	10.1080/22221751.2022.2125348
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Article ; Online: Anti-carbamylated protein antibodies drive AEC II toward a profibrotic phenotype by interacting with carbamylated TLR5.

Xu, Wei / Huang, Minghua / Dong, Rongrong / Yan, Suyan / An, Yan / Liu, Baocheng / Ma, Zhenzhen / Mu, Kun / Yang, Qingrui

Rheumatology (Oxford, England)

2024

Abstract: Objectives: This study was to explore the role of Anti-carbamylated protein (Anti-CarP) antibodies in contributing to lung fibrosis in connective tissue disease (CTD)-associated interstitial lung disease (ILD) in an autoantigen-dependent manner.: ... ...

Abstract	Objectives: This study was to explore the role of Anti-carbamylated protein (Anti-CarP) antibodies in contributing to lung fibrosis in connective tissue disease (CTD)-associated interstitial lung disease (ILD) in an autoantigen-dependent manner. Methods: ELISA tested serum samples, including 89 of CTD-ILD group and 170 of non-ILD CTD, for the anti-CarP levels. Male C57BL/6 mice were used for pulmonary fibrosis model and anti-CarP treatment in vivo (n = 5), and patient serum-derived or commercialized anti-CarP for cell treatment. We identified the carbamylated membrane protein via immunofluorescence (IF) and coimmunoprecipitation followed by mass spectrometry (MS) analysis. RT-qPCR, IF and western blot were performed to explore the antigen-dependent role of anti-CarP. Native electrophoretic mobility shift assay and MS analysis were used to verify direct interaction and carbamylation sites. Results: A significantly higher serum anti-CarP level was observed in CTD with ILD than without ILD. In vivo, intrapulmonary delivery of anti-CarP induces epithelial-mesenchymal transition (EMT) and micro fibrotic foci. Carbamylation was enriched in type II alveolar epithelial cells (AEC II). A novel carbamylated membrane receptor, specifically recognized by anti-CarP, was identified as toll-like receptor 5 (TLR5). We found anti-CarP induces the nuclear translocation of NF-κB and downstream events, including EMT and expression of inflammatory cytokines in AEC II, which were reversed by TLR5 blocking or TLR5 knockdown. Moreover, up to 12 lysine carbamylation sites were found in TLR5 ectodomain, allowing the interaction of anti-CarP with carbamylated TLR5. Conclusions: Overall, we found anti-CarP drives aberrant AEC II activation by interacting with carbamylated TLR5 to promote ILD progress.
Language	English
Publishing date	2024-02-16
Publishing country	England
Document type	Journal Article
ZDB-ID	1464822-2
ISSN	1462-0332 ; 1462-0324
ISSN (online)	1462-0332
ISSN	1462-0324
DOI	10.1093/rheumatology/keae111
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Article ; Online: A non-ACE2-blocking neutralizing antibody against Omicron-included SARS-CoV-2 variants.

Duan, Xiaomin / Shi, Rui / Liu, Pulan / Huang, Qingrui / Wang, Fengze / Chen, Xinyu / Feng, Hui / Huang, Weijin / Xiao, Junyu / Yan, Jinghua

Signal transduction and targeted therapy

2022 Volume 7, Issue 1, Page(s) 23

Language	English
Publishing date	2022-01-25
Publishing country	England
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	2886872-9
ISSN	2059-3635 ; 2095-9907
ISSN (online)	2059-3635
ISSN	2095-9907
DOI	10.1038/s41392-022-00879-2
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Article ; Online: Future carbon storages of ecosystem based on land use change and carbon sequestration practices in a large economic belt.

Huang, Jing / Chen, Qi / Wang, Qingrui / Gao, Jiameng / Yin, Ying / Guo, Hongyan

Environmental science and pollution research international

2023 Volume 30, Issue 39, Page(s) 90924–90935

Abstract: Assessments of ecosystem carbon storage are needed to form the scientific basis for carbon policies. Due to lack of data, there are few accurate, large-scale, and long-term predictions of ecosystem carbon storage. This study used the Distributed Land-Use ...

Abstract	Assessments of ecosystem carbon storage are needed to form the scientific basis for carbon policies. Due to lack of data, there are few accurate, large-scale, and long-term predictions of ecosystem carbon storage. This study used the Distributed Land-Use Change Prediction (DLUCP) model with ten socioeconomic and two climate change scenarios for a total of 20 combinations that take into account population increase, technology innovation, climate change, and Grain for Green Project to make high-resolution predictions of land use change in the Yangtze River Economic Belt. Low and high carbon sequestration practices were considered to predict future carbon densities. Land use change data, carbon densities data, and the InVEST model were used to predict changes in ecosystem carbon storage from now to 2070. The results show a slight increase (1.88-4.17%) in carbon storage in the study area only based on land use change. Grain for Green Project has the largest impact on carbon storage among population increase, technology innovation, climate scenarios, and Grain for Green Project, which increases carbon storage by 4.17%. After the implementation of carbon sequestration practices, there is an increase in carbon storages from 28.51 to 56.77% in the study area from now to 2070, and increasing carbon storages of forest in each stream and carbon storage of cropland in downstream are efficient ways to achieve carbon neutralization.
MeSH term(s)	Ecosystem ; Carbon Sequestration ; Carbon/analysis ; Forests ; Edible Grain/chemistry ; China ; Conservation of Natural Resources
Chemical Substances	Carbon (7440-44-0)
Language	English
Publishing date	2023-07-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-023-28555-0
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Article ; Online: Safety of concomitant administration of inactivated hepatitis A vaccine with other vaccines in children under 16 years old in post-marketing surveillance.

Wu, Linlin / Yang, Shoufei / Huang, Zhuoying / Liu, Jiechen / Guo, Xiang / Bai, Qingrui / Sun, Xiaodong

Vaccine

2023 Volume 41, Issue 14, Page(s) 2412–2417

Abstract: Background: Concomitant administration refers to the receipt of two or more vaccines during a single healthcare encounter, which is an efficient way to increase vaccination coverage in children. However, the post-marketing safety studies of concomitant ... ...

Abstract	Background: Concomitant administration refers to the receipt of two or more vaccines during a single healthcare encounter, which is an efficient way to increase vaccination coverage in children. However, the post-marketing safety studies of concomitant administration are scarce. Inactivated hepatitis A vaccine (Healive®) has been used widely in China and other countries for more than a decade. We aimed to explore the safety of Healive® co-administered with other vaccines compared to Healive® alone in children under 16 years old. Methods: We retrieved Adverse Events Following Immunization (AEFI) cases and vaccination doses of Healive® during 2020-2021 in Shanghai, China. The AEFI cases were divided into concomitant administration group and Healive® alone group. We used administrative data on vaccine doses as denominators to calculate and compare crude reporting rates between groups. We also compared baseline gender and age distribution, clinical diagnoses, and time interval from vaccination to onset of symptoms between groups. Results: A total 319,247 doses of inactivated hepatitis A vaccine (Healive®) were used and 1,020 AEFI cases (319.50 per million doses) associated with Healive® were reported during 2020-2021 in Shanghai. There were 259,346 doses concomitantly administered with other vaccines and 830 AEFI cases (320.04 per million doses) were reported. There were 59,901 doses of Healive® that vaccinated alone, with 190 AEFI cases (317.19 per million doses). There was only one case with serious AEFI in concomitant administration group, with a rate of 0.39 per million doses. Reported rates of AEFI cases were similar between groups in general (p > 0.05). Conclusion: Concomitant administration of inactivated hepatitis A vaccine (Healive®) with other vaccines has a similar safe profile as Healive® alone.
MeSH term(s)	Humans ; Child ; Infant ; Adolescent ; Hepatitis A Vaccines ; Hepatitis A/prevention & control ; China ; Vaccines, Inactivated ; Vaccination/adverse effects ; Marketing ; Product Surveillance, Postmarketing ; Adverse Drug Reaction Reporting Systems
Chemical Substances	Hepatitis A Vaccines ; Vaccines, Inactivated
Language	English
Publishing date	2023-03-03
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2023.02.073
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Article ; Online: Identification and Severity Monitoring of Maize Dwarf Mosaic Virus Infection Based on Hyperspectral Measurements

Lili Luo / Qingrui Chang / Qi Wang / Yong Huang

Remote Sensing, Vol 13, Iss 4560, p

2021 Volume 4560

Abstract: Prompt monitoring of maize dwarf mosaic virus (MDMV) is critical for the prevention and control of disease and to ensure high crop yield and quality. Here, we first analyzed the spectral differences between MDMV-infected red leaves and healthy leaves and ...

Abstract	Prompt monitoring of maize dwarf mosaic virus (MDMV) is critical for the prevention and control of disease and to ensure high crop yield and quality. Here, we first analyzed the spectral differences between MDMV-infected red leaves and healthy leaves and constructed a sensitive index (SI) for measurements. Next, based on the characteristic bands (R λ ) associated with leaf anthocyanins (Anth), we determined vegetation indices (VI s ) commonly used in plant physiological and biochemical parameter inversion and established a vegetation index (VI c ) by utilizing the combination of two arbitrary bands following the construction principles of NDVI, DVI, RVI, and SAVI. Furthermore, we developed classification models based on linear discriminant analysis (LDA) and support vector machine (SVM) in order to distinguish the red leaves from healthy leaves. Finally, we performed UR, MLR, PLSR, PCR, and SVM simulations on Anth based on R λ , VI s , VI c , and R λ + VI s + VI c and indirectly estimated the severity of MDMV infection based on the relationship between the reflection spectra and Anth. Distinct from those of the normal leaves, the spectra of red leaves showed strong reflectance characteristics at 640 nm, and SI increased with increasing Anth. Moreover, the accuracy of the two VI c -based classification models was 100%, which is significantly higher than that of the VI s and R λ -based models. Among the Anth regression models, the accuracy of the MLR model based on R λ + VI s + VI c was the highest (R 2 c = 0.85; R 2 v = 0.74). The developed models could accurately identify MDMV and estimate the severity of its infection, laying the theoretical foundation for large-scale remote sensing-based monitoring of this virus in the future.
Keywords	plant disease ; band selection ; machine learning ; anthocyanin ; hyperspectral reflectance ; linear discriminant analysis ; Science ; Q
Subject code	290
Language	English
Publishing date	2021-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: COVID-19 mRNA vaccines.

Huang, Qingrui / Zeng, Jiawei / Yan, Jinghua

Journal of genetics and genomics = Yi chuan xue bao

2021 Volume 48, Issue 2, Page(s) 107–114

Abstract: The ongoing COVID-19 pandemic and its unprecedented global societal and economic disruptive impact highlight the urgent need for safe and effective vaccines. Taking substantial advantages of versatility and rapid development, two mRNA vaccines against ... ...

Abstract	The ongoing COVID-19 pandemic and its unprecedented global societal and economic disruptive impact highlight the urgent need for safe and effective vaccines. Taking substantial advantages of versatility and rapid development, two mRNA vaccines against COVID-19 have completed late-stage clinical assessment at an unprecedented speed and reported positive results. In this review, we outline keynotes in mRNA vaccine development, discuss recently published data on COVID-19 mRNA vaccine candidates, focusing on those in clinical trials and analyze future potential challenges.
MeSH term(s)	Binding Sites/genetics ; Binding Sites/immunology ; COVID-19/epidemiology ; COVID-19/immunology ; COVID-19/virology ; COVID-19 Vaccines/genetics ; COVID-19 Vaccines/immunology ; COVID-19 Vaccines/therapeutic use ; Drug Development ; Humans ; Pandemics/prevention & control ; RNA, Messenger/genetics ; RNA, Messenger/immunology ; RNA, Messenger/metabolism ; SARS-CoV-2/immunology ; SARS-CoV-2/metabolism ; SARS-CoV-2/physiology ; Vaccines, Synthetic/genetics ; Vaccines, Synthetic/immunology ; Viral Proteins/genetics ; Viral Proteins/immunology ; Viral Proteins/metabolism ; mRNA Vaccines
Chemical Substances	COVID-19 Vaccines ; RNA, Messenger ; Vaccines, Synthetic ; Viral Proteins
Language	English
Publishing date	2021-03-15
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2374568-X
ISSN	1873-5533 ; 1673-8527
ISSN (online)	1873-5533
ISSN	1673-8527
DOI	10.1016/j.jgg.2021.02.006
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Article: Protective effects of paeoniflorin on acrolein-induced apoptosis in H9c2 cardiomyocytes.

Shao, Qingrui / Li, Jianzhe / Huang, Xiaoou / Zhou, Guangyu

Pakistan journal of pharmaceutical sciences

2021 Volume 33, Issue 4, Page(s) 1585–1592

Abstract: Acrolein is a highly toxic unsaturated aldehyde which is abundant in many circumstances. People exposed to acrolein may have significant clinical relevance in human cardiotoxicity situations. Paeoniflorin (PEF) is a bioactive glucoside isolated from the ... ...

Abstract	Acrolein is a highly toxic unsaturated aldehyde which is abundant in many circumstances. People exposed to acrolein may have significant clinical relevance in human cardiotoxicity situations. Paeoniflorin (PEF) is a bioactive glucoside isolated from the roots of Paeonia lactiflora Pall. It is reported that PEF performs a beneficial role in cardiovascular system. The aim of the current research was to evaluate the potential protective effect of PEF against acrolein-induced apoptotic damage in H9c2 cardiomyocytes. This study revealed that PEF exerted a protective effect on acrolein-induced cardiomyocyte apoptosis. Furthermore, treatment with acrolein could markedly increase the levels of reactive oxygen species (ROS) and expression of cleavage of caspase-9 and caspase-3 in H9c2 cardiomyocytes, which were significantly reversed by co-treatment with PEF (100uM). These results demonstrated that PEF protects H9c2 cardiomyocytes against acrolein-induced cardiomyocyte injury via decreasing ROS production and down regulating caspases cascade reaction, indicating that PEF is a potential therapeutic agent for cardiac toxic environmental pollutant injury.
MeSH term(s)	Acrolein/pharmacology ; Animals ; Apoptosis/drug effects ; Caspase 3/metabolism ; Cell Death/drug effects ; Cell Line ; Cell Survival/drug effects ; Down-Regulation/drug effects ; Glucosides/pharmacology ; Monoterpenes/pharmacology ; Myocytes, Cardiac/drug effects ; Myocytes, Cardiac/metabolism ; Oxidative Stress/drug effects ; Protective Agents/pharmacology ; Rats ; Reactive Oxygen Species/metabolism
Chemical Substances	Glucosides ; Monoterpenes ; Protective Agents ; Reactive Oxygen Species ; peoniflorin (21AIQ4EV64) ; Acrolein (7864XYD3JJ) ; Caspase 3 (EC 3.4.22.-)
Language	English
Publishing date	2021-02-15
Publishing country	Pakistan
Document type	Journal Article
ZDB-ID	885131-1
ISSN	1011-601X
ISSN	1011-601X
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Article ; Online: Safety of concomitant administration of inactivated hepatitis A vaccine with other vaccines in children under 16 years old in post-marketing surveillance

Wu, Linlin / Yang, Shoufei / Huang, Zhuoying / Liu, Jiechen / Guo, Xiang / Bai, Qingrui / Sun, Xiaodong

Vaccine.

2023

Abstract: Concomitant administration refers to the receipt of two or more vaccines during a single healthcare encounter, which is an efficient way to increase vaccination coverage in children. However, the post-marketing safety studies of concomitant ... ...

Abstract	Concomitant administration refers to the receipt of two or more vaccines during a single healthcare encounter, which is an efficient way to increase vaccination coverage in children. However, the post-marketing safety studies of concomitant administration are scarce. Inactivated hepatitis A vaccine (Healive®) has been used widely in China and other countries for more than a decade. We aimed to explore the safety of Healive® co-administered with other vaccines compared to Healive® alone in children under 16 years old. We retrieved Adverse Events Following Immunization (AEFI) cases and vaccination doses of Healive® during 2020-2021 in Shanghai, China. The AEFI cases were divided into concomitant administration group and Healive® alone group. We used administrative data on vaccine doses as denominators to calculate and compare crude reporting rates between groups. We also compared baseline gender and age distribution, clinical diagnoses, and time interval from vaccination to onset of symptoms between groups. A total 319,247 doses of inactivated hepatitis A vaccine (Healive®) were used and 1,020 AEFI cases (319.50 per million doses) associated with Healive® were reported during 2020-2021 in Shanghai. There were 259,346 doses concomitantly administered with other vaccines and 830 AEFI cases (320.04 per million doses) were reported. There were 59,901 doses of Healive® that vaccinated alone, with 190 AEFI cases (317.19 per million doses). There was only one case with serious AEFI in concomitant administration group, with a rate of 0.39 per million doses. Reported rates of AEFI cases were similar between groups in general (p>0.05). Concomitant administration of inactivated hepatitis A vaccine (Healive®) with other vaccines is a safe profile compared with Healive® alone.
Keywords	gender ; health services ; hepatitis A ; monitoring ; vaccination ; vaccines ; China ; Vaccine safety ; Adverse events following immunization ; Concomitant administration ; Inactivated hepatitis A vaccine ; Surveillance system
Language	English
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2023.02.073
Database	NAL-Catalogue (AGRICOLA)

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