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  1. Article ; Online: A theoretical simulation of SARS-CoV-2 pooled testing: Pooled sample collection outperforms pooled RNA extraction.

    He, Yugan / Tong, Yigang

    Clinica chimica acta; international journal of clinical chemistry

    2021  Volume 521, Page(s) 155–157

    MeSH term(s) COVID-19 ; Clinical Laboratory Techniques ; Humans ; RNA, Viral/genetics ; SARS-CoV-2 ; Specimen Handling
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-07-16
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2021.07.016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.173: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Importance of sample input volume for accurate SARS-CoV-2 qPCR testing.

    He, Yugan / Xie, Tie / Tu, Qihang / Tong, Yigang

    Analytica chimica acta

    2022  Volume 1199, Page(s) 339585

    Abstract: Nucleic acid testing is the most widely used detection method for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19) pandemic. Currently, a number of COVID-19 real-time ... ...

    Abstract Nucleic acid testing is the most widely used detection method for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19) pandemic. Currently, a number of COVID-19 real-time quantitative reverse transcription PCR (qPCR) kits with high sensitivity and specificity are available for SARS-CoV-2 testing. However, these qPCR assays are not always reliable in detecting low viral load samples (Ct-value ≥ 35), resulting in inconclusive or false-negative results. Here, we used a Poisson distribution to illustrate the inconsistent performance of qPCR tests in detecting low viral load samples. From this, we concluded that the false-negative outcomes resulted from the random occurrences of sampling zero target molecules in a single test, and the probability to sample zero target molecules in one test decreased significantly with increasing purified RNA or initial sample input volume. At a given RNA concentration of 0.5 copy/μL, the probability of sampling zero RNA molecules decreased from 36.79% to close to 0.67% after increasing the RNA input volume from 2 to 10 μL. A SARS-CoV-2 qPCR assay with an LOD of 300 copies/mL was used to validate the improved consistency of the qPCR tests. We found that the false-negative qPCR results of clinical COVID-19 samples with a Ct ≥ 35 decreased by 50% after increasing the input of purified RNA from 2 to 10 μL. The consistency, accuracy, and robustness of nucleic acid testing for SARS-CoV-2 samples with low viral loads can be improved by increasing the sample input volume.
    MeSH term(s) COVID-19/diagnosis ; COVID-19 Testing ; Humans ; Pandemics ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; Sensitivity and Specificity
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2022-02-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2022.339585
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: All-in-one

    He, Yugan / Wang, Liqin / An, Xiaoping / Tong, Yigang

    The Analyst

    2021  Volume 146, Issue 19, Page(s) 6026–6034

    Abstract: The ongoing outbreaks of the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have resulted in unprecedented challenges to global health. To effectively contain the COVID-19 transmission, ...

    Abstract The ongoing outbreaks of the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have resulted in unprecedented challenges to global health. To effectively contain the COVID-19 transmission, rapid tests for detecting existing SARS-CoV-2 infections and assessing virus spread are critical. To address the huge need for ever-increasing tests, we developed a facile all-in-one nucleic acid testing assay by combining Si-OH activated glass bead (aGB)-based viral RNA fast extraction and
    MeSH term(s) COVID-19 ; Colorimetry ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Point-of-Care Testing ; RNA, Viral/genetics ; SARS-CoV-2 ; Sensitivity and Specificity
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-09-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d1an01043c
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2423: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Rapid and highly sensitive one-tube colorimetric RT-LAMP assay for visual detection of SARS-CoV-2 RNA.

    He, Yugan / Xie, Tie / Tong, Yigang

    Biosensors & bioelectronics

    2021  Volume 187, Page(s) 113330

    Abstract: Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a highly contagious disease. To tame the continuously raging outbreak of COVID-19, developing a cheap, rapid and sensitive testing assay is ... ...

    Abstract Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a highly contagious disease. To tame the continuously raging outbreak of COVID-19, developing a cheap, rapid and sensitive testing assay is absolutely imperative. Herein, we developed a one-tube colorimetric RT-LAMP assay for the visual detection of SARS-CoV-2 RNA. The assay integrates Si-OH magnetic beads (MBs)-based fast RNA extraction and rapid isothermal amplification in a single tube, thus bypassing the RNA elution step and directly amplifying on-beads RNA molecules with the visualized results. This one-tube assay has a limit of detection (LOD) as low as 200 copies/mL for sample input volumes of up to 600 μL, and can be performed in less than 1 h from sample collection to result readout. This assay demonstrated a 100% concordance with the gold standard test RT-qPCR test by using 29 clinical specimens and showed high specificity. This one-tube colorimetric RT-LAMP assay can serve as an alternative platform for a rapid and sensitive diagnostic test for COVID-19 and is particularly suitable for use at community clinics or township hospitals.
    MeSH term(s) Biosensing Techniques ; COVID-19 ; Colorimetry ; Diagnostic Tests, Routine ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; RNA, Viral/genetics ; SARS-CoV-2 ; Sensitivity and Specificity
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-05-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2021.113330
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Highly Efficient Photo-Degradation of Gaseous Organic Pollutants Catalyzed by Diatomite-Supported Titanium Dioxide

    Xuefei Liu / Yugan He / Beibei Yang / Qi Yan / Junjiao Yang

    Catalysts, Vol 10, Iss 380, p

    2020  Volume 380

    Abstract: Volatile organic compounds (VOCs) are the most harmful contaminants that have been identified, most of which are gaseous organic pollutants. In this study, TiO 2 @diatomite catalysts with various loading amounts of TiO 2 were fabricated using a facile ... ...

    Abstract Volatile organic compounds (VOCs) are the most harmful contaminants that have been identified, most of which are gaseous organic pollutants. In this study, TiO 2 @diatomite catalysts with various loading amounts of TiO 2 were fabricated using a facile solvothermal method with anhydrous ethanol as a solvent for the removal of VOCs. X-ray diffraction analysis revealed that TiO 2 has an anatase phase and the introduction of diatomite has no negative effect. The catalysts were characterized using scanning electron microscopy and transmittance electron microscopy techniques. The results indicate that after introducing diatomite, TiO 2 nanoparticles are mostly square-like and intact, and are uniformly immobilized in the diatomite. Finally, their photocatalytic performance was investigated using liquid ultraviolet spectrometry and gas chromatography-mass spectrometry. Among the catalysts tested, 0.35TiO 2 @diatomite (with a mass ratio of TiO 2 to diatomite of 0.35) exhibited higher photocatalytic activity than the other samples, i.e., pure TiO 2 and diatomite, and could effectively remove acetone and benzene, demonstrating its potential market application and practical significance.
    Keywords gaseous organic pollutants ; titanium dioxide ; diatomite ; photocatalysis ; volatile organic compounds ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Human parainfluenza 3 and respiratory syncytial viruses detected in pangolins.

    Que, Tengcheng / Li, Jing / He, Yugan / Chen, Panyu / Lin, Wei / He, Meihong / Yu, Lei / Wu, Aiqiong / Tan, Luohao / Li, Yingjiao / Hu, Yanling / Tong, Yigang

    Emerging microbes & infections

    2022  Volume 11, Issue 1, Page(s) 1657–1663

    Abstract: Pangolins have gained increasing global attention owing to their public health significance as potential zoonotic hosts since the identification of SARS-CoV-2-related viruses in them. Moreover, these animals could carry other respiratory viruses. In this ...

    Abstract Pangolins have gained increasing global attention owing to their public health significance as potential zoonotic hosts since the identification of SARS-CoV-2-related viruses in them. Moreover, these animals could carry other respiratory viruses. In this study, we investigated the virome composition of 16 pangolins that died in 2018 with symptoms of pneumonia using metagenomic approaches. A total of eight whole virus sequences belonging to the
    MeSH term(s) Animals ; COVID-19 ; Humans ; Pangolins ; Parainfluenza Virus 3, Human/genetics ; Paramyxoviridae Infections ; Phylogeny ; Respiratory Syncytial Virus, Human/genetics ; SARS-CoV-2
    Language English
    Publishing date 2022-07-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2681359-2
    ISSN 2222-1751 ; 2222-1751
    ISSN (online) 2222-1751
    ISSN 2222-1751
    DOI 10.1080/22221751.2022.2086071
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: A novel virus in the family Marnaviridae as a potential pathogen of Penaeus vannamei glass post-larvae disease.

    Xu, Ailan / Xu, Shan / Tu, Qihang / Qiao, Huanao / Lin, Wei / Li, Jing / He, Yugan / Xie, Tie / Pan, Lingting / Pan, Qiang / Zhao, Yunwei / Su, Xin / Tong, Yigang

    Virus research

    2022  Volume 324, Page(s) 199026

    Abstract: As an aquatic animal of great commercial relevance, Penaeus vannamei is currently the dominant species of cultured shrimp in China and many other countries worldwide. In recent years, the outbreak of glass post-larvae disease (GPD), which accounts for ... ...

    Abstract As an aquatic animal of great commercial relevance, Penaeus vannamei is currently the dominant species of cultured shrimp in China and many other countries worldwide. In recent years, the outbreak of glass post-larvae disease (GPD), which accounts for more than 90% of the mortality of shrimp seedlings in serious cases, in many regions of China has caused significant losses and threatened the sustainability of the aquaculture industry and the economy. It is extremely urgent to determine the infectious agent of GPD in P. vannamei. In this work, we performed metagenomic sequencing of glass post-larvae collected from diseased prawns in Tangshan Hebei, where GPD broke out recently. An evolutionary tree was constructed by MEGA 7 to understand the evolutionary history and relationship of the pathogen genome. A novel virus in the family Marnaviridae was first identified in P. vannamei suffering from GPD, and we tentatively named this virus Baishivirus (GenBank: ON550424). The identified pathogen was validated according to Koch's rule with a pathogenic challenge assay and reverse transcription-polymerase chain reaction. There was only 8% query coverage with 64.96% identity in the Baishivirus genome when compared with its most closely related genome sequence of Wenzhou picorna-like virus 21 reported in 2016. Baishivirus genomic RNA is 9.895 kb in length and encodes three potential open reading frames (ORFs). The identification of Baishivirus in P. vannamei enriches the family Marnaviridae and potentially provides a new candidate to study and prevent GPD in the aquaculture industry.
    MeSH term(s) Animals ; Penaeidae ; RNA Viruses ; Genome ; Genomics ; China
    Language English
    Publishing date 2022-12-15
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2022.199026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1965: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Flood Monitoring Using Sentinel-1 SAR for Agricultural Disaster Assessment in Poyang Lake Region

    Hengkai Li / Zikun Xu / Yanbing Zhou / Xiaoxing He / Minghua He

    Remote Sensing, Vol 15, Iss 21, p

    2023  Volume 5247

    Abstract: ... affected areas were primarily concentrated in Yongxiu County, Poyang County, Xinjian District, and Yugan ...

    Abstract An extensive number of farmlands in the Poyang Lake region of China have been submerged due to the impact of flood disasters, resulting in significant agricultural economic losses. Therefore, it is of great importance to conduct the long-term temporal monitoring of flood-induced water body changes using remote sensing technology. However, the scarcity of optical images and the complex, fragmented terrain are pressing issues in the current water body extraction efforts in southern hilly regions, particularly due to difficulties in distinguishing shadows from numerous mountain and water bodies. For this purpose, this study employs Sentinel-1 synthetic aperture radar (SAR) data, complemented by water indices and terrain features, to conduct research in the Poyang Lake area. The results indicate that the proposed multi-source data water extraction method based on microwave remote sensing data can quickly and accurately extract a large range of water bodies and realize long-time monitoring, thus proving a new technical means for the accurate extraction of floodwater bodies in the Poyang Lake region. Moreover, the comparison of several methods reveals that CAU-Net, which utilizes multi-band imagery as the input and incorporates a channel attention mechanism, demonstrated the best extraction performance, achieving an impressive overall accuracy of 98.71%. This represents a 0.12% improvement compared to the original U-Net model. Moreover, compared to the thresholding, decision tree, and random forest methods, CAU-Net exhibited a significant enhancement in extracting flood-induced water bodies, making it more suitable for floodwater extraction in the hilly Poyang Lake region. During this flood monitoring period, the water extent in the Poyang Lake area rapidly expanded and subsequently declined gradually. The peak water area reached 4080 km 2 at the height of the disaster. The severely affected areas were primarily concentrated in Yongxiu County, Poyang County, Xinjian District, and Yugan County.
    Keywords Sentinel-1 ; water extraction ; flood disaster ; decision tree ; random forest ; improved U-Net ; Science ; Q
    Subject code 333
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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