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  1. Article ; Online: Main-chain heterobimetallic block copolymers: synthesis and self-assembly of polyferrocenylsilane-b-poly(cobaltoceniumethylene).

    Gilroy, Joe B / Patra, Sanjib K / Mitchels, John M / Winnik, Mitchell A / Manners, Ian

    Angewandte Chemie (International ed. in English)

    2011  Volume 50, Issue 26, Page(s) 5851–5855

    Language English
    Publishing date 2011-06-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.201008184
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET.

    Yang, Jae / Vrbovská, Veronika / Franke, Tilman / Sibert, Bryan / Larson, Matthew / Hall, Alex / Rigort, Alex / Mitchels, John / Wright, Elizabeth R

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Correlative cryo-FLM-FIB milling is a powerful sample preparation technique ... ...

    Abstract Correlative cryo-FLM-FIB milling is a powerful sample preparation technique for
    Language English
    Publishing date 2023-07-12
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.07.11.548578
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Precise 3D Localization by Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET.

    Yang, Jae / Vrbovská, Veronika / Franke, Tilman / Sibert, Bryan / Larson, Matt / Coomes, Tom / Rigort, Alexander / Mitchels, John / Wright, Elizabeth R

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue Supplement_1, Page(s) 1055–1057

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.541
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  4. Article ; Online: Plasma FIB milling for the determination of structures in situ.

    Berger, Casper / Dumoux, Maud / Glen, Thomas / Yee, Neville B-Y / Mitchels, John M / Patáková, Zuzana / Darrow, Michele C / Naismith, James H / Grange, Michael

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 629

    Abstract: Structural biology studies inside cells and tissues require methods to thin vitrified specimens to electron transparency. Until now, focused ion beams based on gallium have been used. However, ion implantation, changes to surface chemistry and an ... ...

    Abstract Structural biology studies inside cells and tissues require methods to thin vitrified specimens to electron transparency. Until now, focused ion beams based on gallium have been used. However, ion implantation, changes to surface chemistry and an inability to access high currents limit gallium application. Here, we show that plasma-coupled ion sources can produce cryogenic lamellae of vitrified human cells in a robust and automated manner, with quality sufficient for pseudo-atomic structure determination. Lamellae were produced in a prototype microscope equipped for long cryogenic run times (> 1 week) and with multi-specimen support fully compatible with modern-day transmission electron microscopes. We demonstrate that plasma ion sources can be used for structural biology within cells, determining a structure in situ to 4.9 Å, and characterise the resolution dependence on particle distance from the lamella edge. We describe a workflow upon which different plasmas can be examined to further streamline lamella fabrication.
    MeSH term(s) Humans ; Microscopy ; Electrons ; Workflow ; Carmustine
    Chemical Substances Carmustine (U68WG3173Y)
    Language English
    Publishing date 2023-02-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-36372-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Plasma FIB milling for the determination of structures in situ

    Casper Berger / Maud Dumoux / Thomas Glen / Neville B.-y. Yee / John M. Mitchels / Zuzana Patáková / Michele C. Darrow / James H. Naismith / Michael Grange

    Nature Communications, Vol 14, Iss 1, Pp 1-

    2023  Volume 12

    Abstract: The authors harness plasma focused ion beams for pseudo-atomic structure determination, reporting increased throughput and automation in in situ structural biology to elucidate structure-function relationships inside cells and tissues. ...

    Abstract The authors harness plasma focused ion beams for pseudo-atomic structure determination, reporting increased throughput and automation in in situ structural biology to elucidate structure-function relationships inside cells and tissues.
    Keywords Science ; Q
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Advanced cryo-tomography workflow developments - correlative microscopy, milling automation and cryo-lift-out.

    Kuba, Jakub / Mitchels, John / Hovorka, MiloŠ / Erdmann, Philipp / Berka, LukÁŠ / Kirmse, Robert / KÖnig, Julia / DE Bock, Jan / Goetze, Bernhard / Rigort, Alexander

    Journal of microscopy

    2020  Volume 281, Issue 2, Page(s) 112–124

    Abstract: Cryo-electron tomography (cryo-ET) is a groundbreaking technology for 3D visualisation and analysis of biomolecules in the context of cellular structures. It allows structural investigations of single proteins as well as their spatial arrangements within ...

    Abstract Cryo-electron tomography (cryo-ET) is a groundbreaking technology for 3D visualisation and analysis of biomolecules in the context of cellular structures. It allows structural investigations of single proteins as well as their spatial arrangements within the cell. Cryo-tomograms provide a snapshot of the complex, heterogeneous and transient subcellular environment. Due to the excellent structure preservation in amorphous ice, it is possible to study interactions and spatial relationships of proteins in their native state without interference caused by chemical fixatives or contrasting agents. With the introduction of focused ion beam (FIB) technology, the preparation of cellular samples for electron tomography has become much easier and faster. The latest generation of integrated FIB and scanning electron microscopy (SEM) instruments (dual beam microscopes), specifically designed for cryo-applications, provides advances in automation, imaging and the preparation of high-pressure frozen bulk samples using cryo-lift-out technology. In addition, correlative cryo-fluorescence microscopy provides cellular targeting information through integrated software and hardware interfaces. The rapid advances, based on the combination of correlative cryo-microscopy, cryo-FIB and cryo-ET, have already led to a wealth of new insights into cellular processes and provided new 3D image data of the cell. Here we introduce our recent developments within the cryo-tomography workflow, and we discuss the challenges that lie ahead. LAY DESCRIPTION: This article describes our recent developments for the cryo-electron tomography (cryo-ET) workflow. Cryo-ET offers superior structural preservation and provides 3D snapshots of the interior of vitrified cells at molecular resolution. Before a cellular sample can be imaged by cryo-ET, it must be made accessible for transmission electron microscopy. This is achieved by preparing a 200-300 nm thin cryo-lamella from the cellular sample using a cryo-focused ion beam (cryo-FIB) microscope. Cryo-correlative light and electron microscopy (cryo-CLEM) is used within the workflow to guide the cryo-lamella preparation to the cellular areas of interest. We cover a basic introduction of the cryo-ET workflow and show new developments for cryo-CLEM, which facilitate the connection between the cryo-light microscope and the cryo-FIB. Next, we present our progress in cryo-FIB software automation to streamline cryo-lamella preparation. In the final section we demonstrate how the cryo-FIB can be used for 3D imaging and how bulk-frozen cellular samples (obtained by high-pressure freezing) can be processed using the newly developed cryo-lift-out technology.
    MeSH term(s) Automation ; Cryoelectron Microscopy ; Electron Microscope Tomography ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Workflow
    Language English
    Publishing date 2020-07-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 219263-9
    ISSN 1365-2818 ; 0022-2720
    ISSN (online) 1365-2818
    ISSN 0022-2720
    DOI 10.1111/jmi.12939
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 838: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  7. Article: Shedding Light Onto the Nature of Iron Decorated Graphene and Graphite Oxide Nanohybrids for CO

    Owen, Rhodri E / Cortezon-Tamarit, Fernando / Calatayud, David G / Evans, Enid A / Mitchell, Samuel I J / Mao, Boyang / Palomares, Francisco J / Mitchels, John / Plucinski, Pawel / Mattia, Davide / Jones, Matthew D / Pascu, Sofia I

    ChemistryOpen

    2020  Volume 9, Issue 2, Page(s) 242–252

    Abstract: We report on the design and testing of new graphite and graphene oxide-based extended π-conjugated synthetic scaffolds for applications in sustainable chemistry transformations. Nanoparticle-functionalised carbonaceous catalysts for new Fischer Tropsch ... ...

    Abstract We report on the design and testing of new graphite and graphene oxide-based extended π-conjugated synthetic scaffolds for applications in sustainable chemistry transformations. Nanoparticle-functionalised carbonaceous catalysts for new Fischer Tropsch and Reverse GasWater Shift (RGWS) transformations were prepared: functional graphene oxides emerged from graphite powders via an adapted Hummer's method and subsequently impregnated with uniform-sized nanoparticles. Then the resulting nanomaterials were imaged by TEM, SEM, EDX, AFM and characterised by IR, XPS and Raman spectroscopies prior to incorporation of Pd(II) promoters and further microscopic and spectroscopic analysis. Newly synthesised 2D and 3D layered nanostructures incorporating carbon-supported iron oxide nanoparticulate pre-catalysts were tested, upon hydrogen reduction
    Language English
    Publishing date 2020-02-14
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2655605-4
    ISSN 2191-1363
    ISSN 2191-1363
    DOI 10.1002/open.201900368
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  8. Article: ABC of child abuse. Protecting the child.

    Mitchels, B

    BMJ (Clinical research ed.)

    1989  Volume 299, Issue 6698, Page(s) 559–562

    MeSH term(s) Child ; Child Abuse/legislation & jurisprudence ; Child Abuse/prevention & control ; Child Advocacy ; Child Custody ; Child, Preschool ; Humans ; Infant
    Language English
    Publishing date 1989-08-26
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1362901-3
    ISSN 1756-1833 ; 0959-8138 ; 0959-8154 ; 0959-8146 ; 0959-535X ; 1759-2151
    ISSN (online) 1756-1833
    ISSN 0959-8138 ; 0959-8154 ; 0959-8146 ; 0959-535X ; 1759-2151
    DOI 10.1136/bmj.299.6698.559
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.10: Show issues Location:
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  9. Article: Munchausen syndrome by proxy--protecting the child.

    Mitchels, B

    Journal - Forensic Science Society

    1983  Volume 23, Issue 2, Page(s) 105–111

    MeSH term(s) Child ; Child Abuse/legislation & jurisprudence ; Child Abuse/prevention & control ; Child Care ; Female ; Humans ; Munchausen Syndrome/complications ; Munchausen Syndrome/therapy
    Language English
    Publishing date 1983-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 800369-5
    ISSN 0015-7368
    ISSN 0015-7368
    DOI 10.1016/s0015-7368(83)72218-8
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 854: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  10. Book: The Children Act and medical practice

    Mitchels, Barbara

    (Family law)

    1992  

    Author's details Barbara Mitchels, Alister Prince
    Series title Family law
    MeSH term(s) Child Welfare ; Jurisprudence
    Keywords Great Britain
    Language English
    Size xii, 154 p. :, ill.
    Publisher Jordan
    Publishing place Bristol
    Document type Book
    ISBN 9780853081425 ; 0853081425
    Database Catalogue of the US National Library of Medicine (NLM)

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