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Article ; Online: Probing B-X to B-H conversions and applications in C-F bond activation catalysis.

Yeganeh-Salman, Amir / Elser, Iris / Bamford, Karlee L / Ebanks, Daniel / Stephan, Douglas W

Dalton transactions (Cambridge, England : 2003)

2022 Volume 51, Issue 47, Page(s) 17962–17966

Abstract: Herein we exploit a catalytic amount of [ ... ...

Abstract	Herein we exploit a catalytic amount of [Ph
Language	English
Publishing date	2022-12-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/d2dt03588j
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Article ; Online: Interactions of C-F Bonds with Hydridoboranes: Reduction, Borylation and Friedel-Crafts Alkylation.

Bamford, Karlee L / Chitnis, Saurabh S / Qu, Zheng-Wang / Stephan, Douglas W

Chemistry (Weinheim an der Bergstrasse, Germany)

2018 Volume 24, Issue 60, Page(s) 16014–16018

Abstract: The stoichiometric reactions of the alkylfluorides 1-fluoroadamantane (Ad-F), fluorocyclohexane (Cy ... F), 1-fluoropentane (Pent-F) and benzyl fluorides with secondary boranes pinacolborane (HBpin ...

Abstract	The stoichiometric reactions of the alkylfluorides 1-fluoroadamantane (Ad-F), fluorocyclohexane (Cy-F), 1-fluoropentane (Pent-F) and benzyl fluorides with secondary boranes pinacolborane (HBpin), catecholborane (HBcat), 9-borabicyclo(3.3.1)nonane (9-BBN) and Piers' borane (HB(C
Language	English
Publishing date	2018-10-04
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-x
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.201804705
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Article ; Online: Warming and pollution interact to alter energy transfer efficiency, performance and fitness across generations in zebrafish (Danio rerio).

Seebacher, Frank / Bamford, Stephanie M

The Science of the total environment

2023 Volume 912, Page(s) 168942

Abstract: Energy transfer efficiency across different trophic levels, from food to new biomass, can determine population dynamics and food-web function. Here we show that the energy needed to produce a unit of new biomass increases with warming and exposure to ... ...

Abstract	Energy transfer efficiency across different trophic levels, from food to new biomass, can determine population dynamics and food-web function. Here we show that the energy needed to produce a unit of new biomass increases with warming and exposure to bisphenol A (BPA), an endocrine disrupting compound. These environmental effects are at least partially transmitted across generations via DNA methylation. We raised parental (F0) and their offspring (F1) zebrafish (Danio rerio) of two genotypes (DNA methyltransferase 3a knock-out [DNMT3a
MeSH term(s)	Animals ; Zebrafish/physiology ; DNA Methylation ; Swimming ; Benzhydryl Compounds/toxicity ; Benzhydryl Compounds/metabolism
Chemical Substances	Benzhydryl Compounds
Language	English
Publishing date	2023-12-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.168942
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Zs.A 949: Show issues

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Article ; Online: Sex-specific transgenerational plasticity: developmental temperatures of mothers and fathers have different effects on sons and daughters.

Seebacher, Frank / Bamford, Stephanie M / Le Roy, Amelie

The Journal of experimental biology

2023 Volume 226, Issue 12

Abstract: Each parent can influence offspring phenotype via provisioning of the zygote or sex-specific DNA methylation. Transgenerational plasticity may therefore depend on the environmental conditions experienced by each parent. We tested this hypothesis by ... ...

Abstract	Each parent can influence offspring phenotype via provisioning of the zygote or sex-specific DNA methylation. Transgenerational plasticity may therefore depend on the environmental conditions experienced by each parent. We tested this hypothesis by conducting a fully factorial experiment across three generations of guppies (Poecilia reticulata), determining the effects of warm (28°C) and cold (21°C) thermal backgrounds of mothers and fathers on mass and length, and thermal performance (sustained and sprint swimming speeds, citrate synthase and lactate dehydrogenase activities; 18, 24, 28, 32 and 36°C test temperatures) of sons and daughters. Offspring sex was significant for all traits except for sprint speed. Warmer mothers produced sons and daughters with reduced mass and length, and warmer fathers produced shorter sons. Sustained swimming speed (Ucrit) of male offspring was greatest when both parents were raised at 28°C, and warmer fathers produced daughters with greater Ucrit. Similarly, warmer fathers produced sons and daughters with greater metabolic capacity. We show that the thermal variation experienced by parents can modify offspring phenotype, and that predicting the impacts of environmental change on populations would require knowledge of the thermal background of each mother and father, particularly where sexes are spatially segregated.
MeSH term(s)	Female ; Animals ; Male ; Humans ; Mothers ; Nuclear Family ; Temperature ; Phenotype ; Poecilia ; Fathers
Language	English
Publishing date	2023-06-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218085-6
ISSN	1477-9145 ; 0022-0949
ISSN (online)	1477-9145
ISSN	0022-0949
DOI	10.1242/jeb.245798
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Zs.A 2629: Show issues

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Article ; Online: mSphere of Influence: Interferon Ultra Is a Means To Fight Viral Disease.

Bamford, Connor G G

mSphere

2020 Volume 5, Issue 2

Abstract: ... al. (E. Terczyńska-Dyla, S. Bibert, F. H. T. Duong, I. Krol, et al., Nat Commun 5:5699, 2014, https ... Connor G. G. Bamford works in the fields of virology and innate immunity. In this mSphere ...

Abstract	Connor G. G. Bamford works in the fields of virology and innate immunity. In this mSphere of Influence article, he reflects on how three papers by Manry et al. (J. Manry, G. Laval, E. Patin, S. Fornarino, et al., J Exp Med 208:2747-2759, 2011, https://doi.org/10.1084/jem.20111680), Terczyńska-Dyla et al. (E. Terczyńska-Dyla, S. Bibert, F. H. T. Duong, I. Krol, et al., Nat Commun 5:5699, 2014, https://doi.org/10.1038/ncomms6699), and Schoggins et al. (J. W. Schoggins, S. J. Wilson, M. Panis, M. Y. Murphy, et al., Nature 472:481-485, 2011, https://doi.org/10.1038/nature09907) made an impact on him during a critical stage of his career and how they continue to reinforce why he researches what he does and shape the approaches he takes.
MeSH term(s)	Humans ; Interferons/therapeutic use ; Virus Diseases/therapy
Chemical Substances	Interferons (9008-11-1)
Language	English
Publishing date	2020-03-25
Publishing country	United States
Document type	Journal Article
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00134-20
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Article ; Online: Evaluation of field-testing protocols to diagnose insulin dysregulation in ponies using a Bayesian approach.

Clark, B L / Stewart, A J / Kemp, K L / Bamford, N J / Bertin, F-R

Veterinary journal (London, England : 1997)

2023 Volume 298-299, Page(s) 106019

Abstract: Field tests and their association with laminitis have not been evaluated in large cohorts. The objectives of this study were to evaluate the performance of basal insulin (BI), the oral sugar test (OST) and the insulin tolerance test (ITT) to diagnose ID ... ...

Abstract	Field tests and their association with laminitis have not been evaluated in large cohorts. The objectives of this study were to evaluate the performance of basal insulin (BI), the oral sugar test (OST) and the insulin tolerance test (ITT) to diagnose ID and investigate their association with laminitis. Insulin dysregulation status was determined in 146 ponies using BI (insulin concentration >20 µIU/mL), an OST (insulin concentration >65 µIU/mL at 60 or 90 min after oral administration of 0.45 mL/kg corn syrup) and an ITT (< 50% reduction in glucose concentration 30 min after intravenous administration of 0.1 IU/kg insulin). Laminitis was identified using modified-Obel scores. A Bayesian approach was used to define the characteristics of the tests and receiver operating characteristic curves were used to assess their association with laminitis. All tests were well tolerated and laminitis was diagnosed in 9% of ponies. Insulin dysregulation was diagnosed in 15% of ponies using BI, 38% using the OST and 54% using the ITT with 11% of ponies positive for all three tests. The sensitivities and specificities of BI, the OST and the ITT to diagnose ID were 0.52 (95% confidence interval [CI], 0.35-0.79) and 0.97 (95% CI, 0.91 - 1.00), 0.84 (95% CI, 0.70 - 0.94) and 0.60 (95% CI, 0.49 - 0.71), and 0.85 (95% CI, 0.68-0.96) and 0.88 (95% CI, 0.75 - 0.97), respectively. Only BI and the OST were associated with laminitis (P = 0.003 and 0.015, respectively).
MeSH term(s)	Horses ; Animals ; Insulin ; Blood Glucose ; Glucose Tolerance Test/veterinary ; Bayes Theorem ; Administration, Intravenous/veterinary ; Horse Diseases/diagnosis ; Foot Diseases/diagnosis ; Foot Diseases/veterinary
Chemical Substances	Insulin ; Blood Glucose
Language	English
Publishing date	2023-08-01
Publishing country	England
Document type	Journal Article
ZDB-ID	428614-5
ISSN	1532-2971 ; 0372-5545 ; 1090-0233
ISSN (online)	1532-2971
ISSN	0372-5545 ; 1090-0233
DOI	10.1016/j.tvjl.2023.106019
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Zs.B 552: Show issues

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Article ; Online: Investigation of breed differences in plasma adrenocorticotropic hormone concentrations among healthy horses and ponies.

Bamford, N J / Stewart, A J / El-Hage, C M / Bertin, F R / Bailey, S R

Veterinary journal (London, England : 1997)

2023 Volume 296-297, Page(s) 105995

Abstract: Plasma adrenocorticotropic hormone (ACTH) concentration is commonly measured to diagnose pituitary pars intermedia dysfunction (PPID). Several intrinsic and extrinsic factors affect ACTH concentrations, including breed. The objective of this study was to ...

Abstract	Plasma adrenocorticotropic hormone (ACTH) concentration is commonly measured to diagnose pituitary pars intermedia dysfunction (PPID). Several intrinsic and extrinsic factors affect ACTH concentrations, including breed. The objective of this study was to prospectively compare plasma ACTH concentrations among different breeds of mature horses and ponies. Three breed groups comprised Thoroughbred horses (n = 127), Shetland ponies (n = 131) and ponies of non-Shetland breeds (n = 141). Enrolled animals did not show any signs of illness, lameness or clinical signs consistent with PPID. Blood samples were collected 6 months apart, around the autumn equinox and spring equinox, and plasma concentrations of ACTH were measured by chemiluminescent immunoassay. Pairwise breed comparisons within each season were performed on log transformed data using the Tukey test. Estimated mean differences in ACTH concentrations were expressed as fold difference with 95 % confidence intervals (CI). Reference intervals for each breed group per season were calculated using non-parametric methods. In autumn, higher ACTH concentrations were found among non-Shetland pony breeds compared with Thoroughbreds (1.55 fold higher; 95 % CI, 1.35-1.77; P < 0.001), and in Shetland ponies compared with Thoroughbreds (2.67 fold higher; 95 % CI, 2.33-3.08; P < 0.001) and non-Shetland pony breeds (1.73 fold higher; 95 % CI, 1.51-1.98; P < 0.001). In spring, no differences were identified among breed groups (all P > 0.05). Reference intervals were similar among breed groups in spring, but upper limits for ACTH concentrations were markedly different between Thoroughbred horses and pony breeds in autumn. These findings emphasise that breed should be accounted for when determining and interpreting reference intervals for ACTH concentrations among healthy horses and ponies in autumn.
MeSH term(s)	Horses/genetics ; Animals ; Horse Diseases/genetics ; Pituitary Diseases/genetics ; Pituitary Diseases/veterinary ; Adrenocorticotropic Hormone ; Seasons ; Gait
Chemical Substances	Adrenocorticotropic Hormone (9002-60-2)
Language	English
Publishing date	2023-05-18
Publishing country	England
Document type	Journal Article
ZDB-ID	428614-5
ISSN	1532-2971 ; 0372-5545 ; 1090-0233
ISSN (online)	1532-2971
ISSN	0372-5545 ; 1090-0233
DOI	10.1016/j.tvjl.2023.105995
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Zs.B 552: Show issues

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Article ; Online: Single-center experience evaluating and initiating people with HIV on long-acting cabotegravir/rilpivirine.

Hill, Lucas A / Abulhosn, Kari K / Yin, Jeffrey F / Bamford, Laura P

AIDS (London, England)

2022 Volume 37, Issue 4, Page(s) 605–609

Abstract: Objective: To describe our experience evaluating and initiating individuals on long-acting injectable (LAI) cabotegravir/rilpivirine (CAB/RPV) and evaluate factors associated with starting LAI CAB/RPV and reasons for not starting.: Design: We ... ...

Abstract	Objective: To describe our experience evaluating and initiating individuals on long-acting injectable (LAI) cabotegravir/rilpivirine (CAB/RPV) and evaluate factors associated with starting LAI CAB/RPV and reasons for not starting. Design: We conducted a retrospective single-center study at the UC San Diego Owen Clinic. Methods: We included all individuals who expressed interest in treatment with LAI CAB/RPV between April 2021 and June 2022 who had a definitive decision made on starting LAI CAB/RPV. Results: In total, 383 individuals were included with 201 (52.5%) initiating LAI CAB/RPV. Those who initiated LAI CAB/RPV were younger ( P = 0.02) and were more likely to be on a two-drug regimen or first-generation integrase inhibitor regimen and less likely to be on a protease inhibitor or multiclass regimen. The most common reasons for not starting LAI CAB/RPV were inconsistent clinic attendance or difficulty being contacted and patient choice not to start. Of those who had a proviral DNA resistance test as workup for LAI CAB/RPV ( n = 135), 18.5% had a resistance mutation identified that may have impacted the activity of LAI CAB/RPV. Conclusion: Despite novel challenges over half of our cohort initiated LAI CAB/RPV. Evaluating for potential non-nucleoside reverse transcriptase inhibitor resistance is an important part of the workup for LAI CAB/RPV and proviral DNA resistance testing can be an additional tool to identify potential resistance.
MeSH term(s)	Humans ; Rilpivirine/therapeutic use ; Anti-HIV Agents/therapeutic use ; HIV Infections/drug therapy ; Retrospective Studies ; HIV-1/genetics ; Anti-Retroviral Agents/therapeutic use ; Proviruses
Chemical Substances	Rilpivirine (FI96A8X663) ; Anti-HIV Agents ; cabotegravir (HMH0132Z1Q) ; Anti-Retroviral Agents
Language	English
Publishing date	2022-11-28
Publishing country	England
Document type	Journal Article
ZDB-ID	639076-6
ISSN	1473-5571 ; 0269-9370 ; 1350-2840
ISSN (online)	1473-5571
ISSN	0269-9370 ; 1350-2840
DOI	10.1097/QAD.0000000000003446
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Zs.A 2228: Show issues

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Article ; Online: IL15 and Anti-PD-1 Augment the Efficacy of Agonistic Intratumoral Anti-CD40 in a Mouse Model with Multiple TRAMP-C2 Tumors.

Chen, Wei / Bamford, Richard N / Edmondson, Elijah F / Waldmann, Thomas A

Clinical cancer research : an official journal of the American Association for Cancer Research

2022 Volume 28, Issue 10, Page(s) 2082–2093

Abstract: Purpose: IL15 promotes activation and maintenance of natural killer (NK) and CD8+ T effector memory cells making it a potential immunotherapeutic agent for the treatment of cancer. However, monotherapy with IL15 was ineffective in patients with cancer, ... ...

Abstract	Purpose: IL15 promotes activation and maintenance of natural killer (NK) and CD8+ T effector memory cells making it a potential immunotherapeutic agent for the treatment of cancer. However, monotherapy with IL15 was ineffective in patients with cancer, indicating that it would have to be used in combination with other anticancer agents. The administration of high doses of common gamma chain cytokines, such as IL15, is associated with the generation of "helpless" antigen-nonspecific CD8 T cells. The generation of the tumor-specific cytotoxic T cells can be mediated by CD40 signaling via agonistic anti-CD40 antibodies. Nevertheless, parenteral administration of anti-CD40 antibodies is associated with unacceptable side effects, such as thrombocytopenia and hepatic toxicity, which can be avoided by intratumoral administration. Experimental design: We investigated the combination of IL15 with an intratumoral anti-CD40 monoclonal antibody (mAb) in a dual tumor TRAMP-C2 murine prostate cancer model and expanded the regimen to include an anti-PD-1 mAb. Results: Here we demonstrated that anti-CD40 given intratumorally not only showed significant antitumor activity in treated tumors, but also noninjected contralateral tumors, indicative of abscopal efficacy. The combination of IL15 with intratumoral anti-CD40 showed an additive immune response with an increase in the number of tumor-specific tetramer-positive CD8 T cells. Furthermore, the addition of anti-PD-1 further improved efficacy mediated by the anti-CD40/IL15 combination. Conclusions: These studies support the initiation of a clinical trial in patients with cancer using IL15 in association with the checkpoint inhibitor, anti-PD-1, and intratumoral optimized anti-CD40.
MeSH term(s)	Animals ; Antibodies, Monoclonal/pharmacology ; Antibodies, Monoclonal/therapeutic use ; Antineoplastic Agents/pharmacology ; CD40 Antigens ; CD8-Positive T-Lymphocytes ; Cell Line, Tumor ; Disease Models, Animal ; Humans ; Interleukin-15 ; Male ; Mice ; Mice, Inbred C57BL ; Neoplasms
Chemical Substances	Antibodies, Monoclonal ; Antineoplastic Agents ; CD40 Antigens ; Interleukin-15
Language	English
Publishing date	2022-03-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural
ZDB-ID	1225457-5
ISSN	1557-3265 ; 1078-0432
ISSN (online)	1557-3265
ISSN	1078-0432
DOI	10.1158/1078-0432.CCR-21-0496
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Zs.A 4223: Show issues

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Article ; Online: The power of potential: Assisted reproduction and the counterstories of women who discontinue fertility treatment.

Carson, Andrea / Webster, Fiona / Polzer, Jessica / Bamford, Sandra

Social science & medicine (1982)

2021 Volume 282, Page(s) 114153

Abstract: Despite their low and inconsistent rates of success, assisted reproductive technologies (ARTs) are presented by fertility clinics and constructed in media and popular culture as an effective treatment for infertility. The ways in which such technologies ... ...

Abstract	Despite their low and inconsistent rates of success, assisted reproductive technologies (ARTs) are presented by fertility clinics and constructed in media and popular culture as an effective treatment for infertility. The ways in which such technologies medicalize women's health and bodies have been well documented by social scientists and feminist health researchers. However, little is known about the struggles women face in cases of "failure"; that is, when ART does not achieve its purported potential to assist women in their attempts to conceive and have the desired outcomes of conception and birth. Using a post-structural feminist interpretive framework combined with a narrative methodology, this paper critically examines the ways in which social and cultural narratives about gender and biotechnology shape women's accounts of discontinuing ART. Thirty-six interviews were conducted with twenty-two women across Canada who were at various stages of discontinuation and who utilized a variety of treatment types. Three inter-related narrative themes were developed to categorize the stories of struggle: (i) a growing desperation to be pregnant; (ii) confronting paternalistic medical expectations; and (iii) internalizing and resisting blame for treatment failures. These themes highlight both the explicit and subtle ways in which restrictive social and cultural narratives about womanhood and motherhood were perpetuated in clinical interactions, which ultimately made ending treatment more difficult. Our analysis illustrates how women navigated and resisted such narratives, through pausing or ending treatment despite provider recommendations and clinical messages. We suggest that fertility providers critically reflect on the potentially harmful language used during interactions with patients and recommend that discontinuation discussions become a recurring, normalized component of treatment protocols and patient-provider conversations so that women feel better supported to end treatment when they believe it is financially, emotionally, and physically beneficial for them to do so.
MeSH term(s)	Canada ; Female ; Fertility ; Humans ; Infertility ; Pregnancy ; Reproduction ; Reproductive Techniques, Assisted
Language	English
Publishing date	2021-06-18
Publishing country	England
Document type	Journal Article
ZDB-ID	4766-1
ISSN	1873-5347 ; 0037-7856 ; 0277-9536
ISSN (online)	1873-5347
ISSN	0037-7856 ; 0277-9536
DOI	10.1016/j.socscimed.2021.114153
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