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Article ; Online: Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels

Ali EL BOUKHARI / Salma TABI / Abdelhamid EL MOUSADIK / Rachida EL BOULLANI / Abdelghani TAHIRI / Meriyem KOUFAN / Hamid BENYAHIA / Rachid BOUHARROUD / Naima AIT AABD

Notulae Scientia Biologicae, Vol 15, Iss

2023 Volume 1

Abstract: Argania spinosa L. Skeels is an endemic species of west-central Morocco, which is characterized by a high diversity of morphological and genetic traits. It constitutes a natural resource for oleo-agro-sylvo-pastoral uses. All conservation and genetic ... ...

Abstract	Argania spinosa L. Skeels is an endemic species of west-central Morocco, which is characterized by a high diversity of morphological and genetic traits. It constitutes a natural resource for oleo-agro-sylvo-pastoral uses. All conservation and genetic breeding strategies aimed to domesticate argan require a good knowledge of the plant material. However, several studies focused on agronomical, morphological, phytochemical, and molecular characterization, while the cytogenetic aspects were less investigated. The objective of this work is to identify the chromosome number and ploidy level on the national argan collection at the Agadir Regional Agronomic Research Center, Morocco. The determination of the chromosome number was carried out on root tips of germinated seeds collected from five trees genotypes selected on various morphological aspects. As a result, chromosome count on active root tip cells showed variation in the number (2n = 20; 2n = 22; 2n = 24) with a stable ploidy level (2n = 2x) that is confirmed by flow cytometry. These results combine two previous findings (2n=20, 2n=24) and reveal a third existence of twenty-two chromosome. As a conclusion, A. spinosa has three chromosomal numbers which represent the genetic diversity of the chromosomal number that this species exhibits. More studies are required to explain this variation on chromosome numbers for future breeding programs and to avoid incompatibilities.
Keywords	Argane tree ; chromosome number ; cytogenetic ; flow cytometry ; genetic diversity ; Agriculture (General) ; S1-972 ; Science (General) ; Q1-390
Subject code	580
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	Society of Land Measurements and Cadastre from Transylvania (SMTCT)
Document type	Article ; Online
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Article ; Online: Utility of PCR in Patients with

Blaizot, Romain / Simon, Stephane / Brottier, Jean / Blanchet, Denis / Brousse, Paul / Boukhari, Rachida / Demar, Magalie

The American journal of tropical medicine and hygiene

2019 Volume 101, Issue 4, Page(s) 848–850

Abstract: Strongyloides ... ...

Abstract	Strongyloides stercoralis
MeSH term(s)	Animals ; Anthelmintics/therapeutic use ; Coinfection ; DNA Probes/genetics ; Feces/parasitology ; French Guiana/epidemiology ; HTLV-I Infections/complications ; HTLV-I Infections/epidemiology ; HTLV-I Infections/virology ; Human T-lymphotropic virus 1/immunology ; Humans ; Prevalence ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Strongyloides stercoralis/genetics ; Strongyloides stercoralis/isolation & purification ; Strongyloidiasis/complications ; Strongyloidiasis/diagnosis ; Strongyloidiasis/epidemiology ; Strongyloidiasis/parasitology
Chemical Substances	Anthelmintics ; DNA Probes
Language	English
Publishing date	2019-08-21
Publishing country	United States
Document type	Comparative Study ; Evaluation Study ; Journal Article
ZDB-ID	2942-7
ISSN	1476-1645 ; 0002-9637
ISSN (online)	1476-1645
ISSN	0002-9637
DOI	10.4269/ajtmh.19-0082
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Article ; Online: Shigellosis and Pregnancy in French Guiana: Obstetric and Neonatal Complications.

Parisot, Michaël / Jolivet, Anne / Boukhari, Rachida / Carles, Gabriel

The American journal of tropical medicine and hygiene

2016 Volume 95, Issue 1, Page(s) 26–30

Abstract: Shigella is a major cause of dysentery worldwide. Only a few cases of shigellosis during pregnancy have been reported. However, the neonatal and obstetric complications are potentially severe. The objective of this study was to describe the obstetric and ...

Abstract	Shigella is a major cause of dysentery worldwide. Only a few cases of shigellosis during pregnancy have been reported. However, the neonatal and obstetric complications are potentially severe. The objective of this study was to describe the obstetric and neonatal complications of shigellosis during pregnancy. We carried out a retrospective study of 37 cases of shigellosis diagnosed in pregnant women at the maternity unit of Saint-Laurent du Maroni Hospital in west French Guiana between 2000 and 2014. Shigellosis diagnosis was based on the detection of Shigella in stool cultures from pregnant women (34 patients) or in a neonatal sample collected immediately after delivery (three neonates). In addition to the classic symptoms of shigellosis-an association of diarrhea, fever, and abdominal pain-we observed uterine contractions before the completion of 37 weeks of gestation in 61% of patients (N = 17/28). Cervical changes were associated with uterine contractions in 82% of cases (N = 14/17); 25% of the patients at risk of preterm birth went on to give birth prematurely (N = 3/12). Three cases of mother-to-child transmission were observed. Episodes of shigellosis in pregnant women may trigger uterine contractions and changes to the cervix, potentially resulting in miscarriage or preterm birth.
MeSH term(s)	Abortion, Spontaneous/microbiology ; Adolescent ; Adult ; Anti-Bacterial Agents/therapeutic use ; Cervix Uteri/microbiology ; Drug Resistance, Multiple, Bacterial ; Dysentery, Bacillary/drug therapy ; Dysentery, Bacillary/transmission ; Feces/microbiology ; Female ; French Guiana ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases/drug therapy ; Infant, Newborn, Diseases/microbiology ; Infectious Disease Transmission, Vertical ; Pregnancy ; Pregnancy Complications, Infectious/diagnosis ; Pregnancy Complications, Infectious/drug therapy ; Pregnancy Complications, Infectious/microbiology ; Pregnancy Outcome ; Premature Birth/microbiology ; Retrospective Studies ; Risk Factors ; Shigella/drug effects ; Shigella/isolation & purification ; Young Adult
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2016-07-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	2942-7
ISSN	1476-1645 ; 0002-9637
ISSN (online)	1476-1645
ISSN	0002-9637
DOI	10.4269/ajtmh.15-0669
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Article: ABO Blood Group Incompatibility Protects Against SARS-CoV-2 Transmission.

Boukhari, Rachida / Breiman, Adrien / Jazat, Jennifer / Ruvoën-Clouet, Nathalie / Martinez, Salima / Damais-Cepitelli, Anne / Le Niger, Catherine / Devie-Hubert, Isabelle / Penasse, Fanny / Mauriere, Dominique / Sébille, Véronique / Dürrbach, Antoine / Le Pendu, Jacques

Frontiers in microbiology

2022 Volume 12, Page(s) 799519

Abstract: ABO blood groups appear to be associated with the risk of SARS-CoV-2 infection, but the underlying mechanisms and their real importance remain unclear. Two hypotheses have been proposed: ABO compatibility-dependence (neutralization by anti-ABO antibodies) ...

Abstract	ABO blood groups appear to be associated with the risk of SARS-CoV-2 infection, but the underlying mechanisms and their real importance remain unclear. Two hypotheses have been proposed: ABO compatibility-dependence (neutralization by anti-ABO antibodies) and ABO-dependent intrinsic susceptibility (spike protein attachment to histo-blood group glycans). We tested the first hypothesis through an anonymous questionnaire addressed to hospital staff members. We estimated symptomatic secondary attack rates (SAR) for 333 index cases according to spouse ABO blood group compatibility. Incompatibility was associated with a lower SAR (28% vs. 47%; OR 0.43, 95% CI 0.27-0.69), but no ABO dependence was detected in compatible situations. For the second hypothesis, we detected no binding of recombinant SARS-CoV-2 RBD to blood group-containing glycans. Thus, although no intrinsic differences in susceptibility according to ABO blood type were detected, ABO incompatibility strongly decreased the risk of COVID-19 transmission, suggesting that anti-ABO antibodies contribute to virus neutralization.
Language	English
Publishing date	2022-01-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2021.799519
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Article ; Online: Specificity of Dengue NS1 Antigen in Differential Diagnosis of Dengue and Zika Virus Infection.

Matheus, Séverine / Boukhari, Rachida / Labeau, Bhety / Ernault, Valérie / Bremand, Laetitia / Kazanji, Mirdad / Rousset, Dominique

Emerging infectious diseases

2016 Volume 22, Issue 9, Page(s) 1691–1693

Language	English
Publishing date	2016
Publishing country	United States
Document type	Letter
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2209.160725
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Article ; Online: Specificity of Dengue NS1 Antigen in Differential Diagnosis of Dengue and Zika Virus Infection

Séverine Matheus / Rachida Boukhari / Bhety Labeau / Valérie Ernault / Laetitia Bremand / Mirdad Kazanji / Dominique Rousset

Emerging Infectious Diseases, Vol 22, Iss 9, Pp 1691-

2016 Volume 1693

Keywords	Dengue ; Zika ; cross-reactivity ; nonstructural protein ; NS1 protein ; NS1 antigen ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
Language	English
Publishing date	2016-09-01T00:00:00Z
Publisher	Centers for Disease Control and Prevention
Document type	Article ; Online
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Article ; Online: Population movements and the HIV cascade in recently diagnosed patients at the French Guiana -Suriname border.

Jaries, Raphael / Vantilcke, Vincent / Clevenbergh, Philippe / Adoissi, Jocelyne / Boukhari, Rachida / Misslin, Caroline / Nacher, Mathieu / Vreden, Stephen / Jolivet, Anne

AIDS care

2017 Volume 29, Issue 11, Page(s) 1448–1452

Abstract: Border areas are particular "hot spots" generating high levels of HIV vulnerability and facing great challenges to control epidemics. The objective of this study is to describe the sociodemographic, clinical and biological profiles of newly HIV diagnosed ...

Abstract	Border areas are particular "hot spots" generating high levels of HIV vulnerability and facing great challenges to control epidemics. The objective of this study is to describe the sociodemographic, clinical and biological profiles of newly HIV diagnosed people at the French Guiana - Suriname border, to construct an HIV care cascade and compare it with the Surinamese one. HIV-patients aged over 15 years newly diagnosed in western French Guiana in 2011 and 2012 were included in a retrospective cohort study. Patients were identified using different sources (n = 121). The male-to-female ratio was 0.8, 85% of the patients were of foreign origin, 72% were undocumented migrants, 21% were living in Suriname and 48% had baseline CD4 cell counts <200 cells/mm
MeSH term(s)	Adolescent ; Adult ; Emigrants and Immigrants/statistics & numerical data ; Female ; French Guiana/epidemiology ; HIV Infections/diagnosis ; HIV Infections/epidemiology ; Health Policy ; Humans ; Lost to Follow-Up ; Male ; Middle Aged ; Retrospective Studies ; Suriname/epidemiology ; Transients and Migrants/psychology ; Transients and Migrants/statistics & numerical data ; Young Adult
Language	English
Publishing date	2017-02-13
Publishing country	England
Document type	Journal Article
ZDB-ID	1012651-x
ISSN	1360-0451 ; 0954-0121
ISSN (online)	1360-0451
ISSN	0954-0121
DOI	10.1080/09540121.2017.1291899
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Article: Blood lead levels and risk factors for lead exposure among pregnant women in western French Guiana: the role of manioc consumption.

Rimbaud, Diane / Restrepo, Marion / Louison, Anne / Boukhari, Rachida / Ardillon, Vanessa / Carles, Gabriel / Lambert, Véronique / Jolivet, Anne

Journal of toxicology and environmental health. Part A

2017 Volume 80, Issue 6, Page(s) 382–393

Abstract: Concerns regarding lead (Pb) poisoning in French Guiana first arose in 2011 following the discovery of excessively high levels of the metal amongst children in a small neighborhood without any apparent source of Pb. Since 2012, blood lead level (BLL) ... ...

Abstract	Concerns regarding lead (Pb) poisoning in French Guiana first arose in 2011 following the discovery of excessively high levels of the metal amongst children in a small neighborhood without any apparent source of Pb. Since 2012, blood lead level (BLL) measurement has been proposed for all pregnant women in western French Guiana. The aim of this study was to determine BLL in pregnant women in this region and identify factors associated with elevated BLL. An observational study of a consecutive sample of women who delivered in the maternity ward of the hospital was conducted. Risk factors were investigated using a questionnaire administered postdelivery by midwives (N = 531). Approximately 25 and 5% of women displayed BLL of ≥50 μg/L and ≥100 µg/L, respectively. The geometric mean was 32.6 μg/L. Factors that were significantly associated with an elevated BLL after modeling (multivariate linear regression) included place of residence along the Maroni river, low level of education, daily consumption of manioc derivatives, weekly and daily consumption or personal preparation of manioc flour during pregnancy, and weekly consumption of wild game. This study provides insight into the regional and social disparities in BLL in French Guiana and potential sources of exposure. Evidence indicates that foods that are primarily produced and consumed in the Guiana Shield significantly affect BLL levels. Taken together with existing data, our results demonstrate that specific actions in terms of prevention, screening, and care are required to be adapted and put into place in order to reduce exposure.
MeSH term(s)	Adolescent ; Adult ; Cross-Sectional Studies ; Diet ; Environmental Exposure ; Environmental Monitoring ; Female ; French Guiana ; Humans ; Lead Poisoning/blood ; Manihot/chemistry ; Pregnancy ; Risk Factors ; Young Adult
Language	English
Publishing date	2017
Publishing country	England
Document type	Journal Article
ZDB-ID	1413345-3
ISSN	1528-7394 ; 0098-4108 ; 1087-2620
ISSN	1528-7394 ; 0098-4108 ; 1087-2620
DOI	10.1080/15287394.2017.1331490
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Article ; Online: Fever in hospitalized HIV-infected patients in Western French Guiana: first think histoplasmosis.

Vantilcke, Vincent / Boukhari, Rachida / Jolivet, Anne / Vautrin, Cyrille / Misslin, Caroline / Adenis, Antoine / Nacher, Mathieu

International journal of STD & AIDS

2014 Volume 25, Issue 9, Page(s) 656–661

Abstract: In Western French Guiana, there was a dramatic increase in HIV prevalence between 1990 and 2000. The present study describes the causes of fever among HIV patients hospitalized in the medical ward of the only hospital in the western part of French Guiana. ...

Abstract	In Western French Guiana, there was a dramatic increase in HIV prevalence between 1990 and 2000. The present study describes the causes of fever among HIV patients hospitalized in the medical ward of the only hospital in the western part of French Guiana. A retrospective descriptive study was conducted between 1 January 2008 and 30 June 2010 in the department of medicine of Saint Laurent du Maroni Hospital. The main characteristics of 67 patients having presented with fever in the first 48 hours of hospitalization were described. Among patients with CD4 <200/mm(3)the main febrile opportunistic infection was disseminated histoplasmosis (41.1%). Among patients with CD4 counts <50/mm(3)and fever without focal points 85.7% had disseminated histoplasmosis. Three patients died and all had disseminated histoplasmosis. Disseminated histoplasmosis is the most common febrile opportunistic infection in western French Guiana. Primary prophylaxis with itraconazole among immunocompromised patients seems warranted.
MeSH term(s)	AIDS-Related Opportunistic Infections/diagnosis ; AIDS-Related Opportunistic Infections/drug therapy ; AIDS-Related Opportunistic Infections/mortality ; Adolescent ; Adult ; Age Distribution ; Aged ; Amphotericin B/therapeutic use ; CD4 Lymphocyte Count ; Coinfection ; Female ; Fever/etiology ; French Guiana/epidemiology ; HIV Infections/complications ; HIV Infections/virology ; Histoplasma/isolation & purification ; Histoplasmosis/diagnosis ; Histoplasmosis/drug therapy ; Histoplasmosis/mortality ; Hospitalization/statistics & numerical data ; Humans ; Immunocompromised Host ; Itraconazole/therapeutic use ; Male ; Middle Aged ; Prevalence ; Retrospective Studies ; Sex Distribution ; Weight Loss ; Young Adult
Chemical Substances	liposomal amphotericin B ; Itraconazole (304NUG5GF4) ; Amphotericin B (7XU7A7DROE)
Language	English
Publishing date	2014-08
Publishing country	England
Document type	Journal Article
ZDB-ID	1018089-8
ISSN	1758-1052 ; 0956-4624
ISSN (online)	1758-1052
ISSN	0956-4624
DOI	10.1177/0956462413516299
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Article ; Online: Incidence of infantile Pompe disease in the Maroon population of French Guiana.

Elenga, Narcisse / Verloes, Alain / Mrsic, Yajaira / Basurko, Célia / Schaub, Roxane / Cuadro-Alvarez, Emma / Kom-Tchameni, Rémi / Carles, Gabriel / Lambert, Véronique / Boukhari, Rachida / Fahrasmane, Aniza / Jolivet, Anne / Nacher, Mathieu / Benoist, Jean-François

BMJ paediatrics open

2018 Volume 2, Issue 1, Page(s) e000182

Abstract: Objectives: The aim of this study was to describe the epidemiology of infantile Pompe disease (IPD) in French Guiana, a French overseas territory, by combining a retrospective case records study and a prospective anonymous genotyping in a sample of ... ...

Abstract	Objectives: The aim of this study was to describe the epidemiology of infantile Pompe disease (IPD) in French Guiana, a French overseas territory, by combining a retrospective case records study and a prospective anonymous genotyping in a sample of mothers followed in the two major maternity units of French Guiana. Methods: We identified 19 newborns with IPD born within a 13-year-period in French Guiana, corresponding to 1/4528 births. All children were born within the African-American Maroon (Bushinengue) community originating from slaves who settled along the Maroni river in the 19th century. We also performed an anonymised screening for all women in postpartum, in the two main maternity units of French Guiana. Results: Genetic investigations revealed that all patients with IPD were homozygotes or compound heterozygotes for two known pathogenic variations: c.2560C>T p.(Arg854) that has already been reported in African-Americans and c.1942G>A p.(Gly648Ser), a rare previously considered to be variant. We identified no heterozygotes among 453 mothers of various ethnicities in Cayenne, but 15 heterozygotes among 425 mothers (1/27) in Saint-Laurent-du-Maroni (95% CI 1/45 to 1/17), all from the Maroon community, which corresponds to an expected IPD incidence in Maroons of 1/1727 (95% CI 1/1156 to 1/8100). Conclusion:* The incidence of IPD in the Maroon community is roughly 50 times higher than elsewhere in the world. The presence of only two different variants in all affected patients is compatible with a double founder effect in a relatively small population that has seldom mixed with other regional populations in the past and therefore has a reduced pool of genotypes.
Language	English
Publishing date	2018-01-09
Publishing country	England
Document type	Journal Article
ISSN	2399-9772
ISSN (online)	2399-9772
DOI	10.1136/bmjpo-2017-000182
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