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Article ; Online: Preclinical testing of a prototype medical device to treat cervical insufficiency.

House, Michael / Campbell, Devon C / Hickman, Dwayne / Laing, Genevieve / Craigo, Sabrina

American journal of obstetrics & gynecology MFM

2023 Volume 6, Issue 1, Page(s) 101236

Language	English
Publishing date	2023-11-23
Publishing country	United States
Document type	Letter
ISSN	2589-9333
ISSN (online)	2589-9333
DOI	10.1016/j.ajogmf.2023.101236
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Article ; Online: Performance and Acceptability of a New Dexpanthenol-Containing Hand Cream in Subjects with Sensitive and Very Dry Skin

Hans Stettler / Raffaella de Salvo / Marianne Brandt / Ann-Kathrin Effertz / Sabrina Laing / Sonja Trapp

Cosmetics, Vol 9, Iss 44, p

A Randomized Controlled Study

2022 Volume 44

Abstract: A new dexpanthenol-containing hand cream (ND-HC) was developed for people with dry, sensitive, and/or environmentally stressed hands. To explore the performance and acceptability of ND-HC, we conducted a randomized, intraindividual comparison study in 40 ...

Abstract	A new dexpanthenol-containing hand cream (ND-HC) was developed for people with dry, sensitive, and/or environmentally stressed hands. To explore the performance and acceptability of ND-HC, we conducted a randomized, intraindividual comparison study in 40 healthy adult subjects with sensitive and very dry skin on the hands. Instrumental measurements determined the effects on stratum corneum (SC) hydration and transepidermal water loss (TEWL) after single and/or 4 weeks’ use of ND-HC. Single and continued at least four times daily applications of ND-HC to very dry skin of the hand for 4 weeks triggered significant increases in SC hydration. On day 29, the mean change in skin capacitance from baseline was significantly greater when ND-HC was applied to the test area compared with the untreated area on the contralateral hand (12.41 vs. 4.46 a.u.; p < 0.001). Upon use of ND-HC over 4 weeks, mean TEWL decreased significantly (bilateral difference: −1.8 vs. 1.0 g/m 2 /h; p = 0.003), indicating an improvement in SC barrier function. A reduction in dry hand symptoms was observed over the study course. ND-HC was well tolerated and achieved a high level of acceptance and satisfaction. Our findings suggest that ND-HC complies with the required features of a state-of-the-art hand cream.
Keywords	dexpanthenol ; dry skin ; hand cream ; moisturization ; skin barrier ; study ; Chemistry ; QD1-999
Subject code	571
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Anti-pollution effects of two antioxidants and a chelator-Ex vivo electron spin resonance and in vivo cigarette smoke model assessments in human skin.

Bielfeldt, Stephan / Jung, Katinka / Laing, Sabrina / Moga, Alain / Wilhelm, Klaus-Peter

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

2021 Volume 27, Issue 6, Page(s) 1092–1099

Abstract: Background: Skin damage arising from pollutants in gaseous and particulate matter forms is mainly mediated by oxidative stress. The pollutants directly or indirectly generate free radicals on and in the skin, leading, for example, to MMP up-regulation ... ...

Abstract	Background: Skin damage arising from pollutants in gaseous and particulate matter forms is mainly mediated by oxidative stress. The pollutants directly or indirectly generate free radicals on and in the skin, leading, for example, to MMP up-regulation and damage of collagen fibers. Antioxidants and chelators are used in anti-pollution cosmetics to reduce the harmful effects of free radical generation. Materials and methods: We investigated the efficacy of two antioxidants and one chelator in an anti-pollution cigarette smoke model. Free radical generation was measured directly after UV and cigarette smoke exposure ex vivo on pig skin (slaughterhouse waste), by use of Electron Spin Resonance (ESR). Effects of cigarette smoke were compared to those of Urban Dust (NIST-standard). ESR was also used to measure the copper chelation activity of the test products. Following cigarette smoke application in vivo, two markers of lipid peroxidation malondialdehyde (MDA), and squalene monohydroperoxide (SQOOH), were measured from swab solutions taken from the smoke-exposed skin sites. Results: EDTA generated no effect and the non-chelator antioxidant Tocopherol only small antioxidant effects after exposed to cigarette smoke ex vivo as well as in vivo. Only the hydrophilic phenylethanoid H1 showed significant effects. A clear reduction of free radicals ex vivo and further a significant reduction of in vivo lipid peroxide formation was measured. Conclusion: The cigarette smoke model is an ideal method for in vivo assessment of anti-pollution efficacy of topical products with close relation to the real situation of subjects exposed to urban pollution. Further research is required to better understand the role of chelators in anti-pollution cosmetics.
MeSH term(s)	Animals ; Antioxidants/pharmacology ; Chelating Agents ; Electron Spin Resonance Spectroscopy ; Humans ; Smoke/adverse effects ; Smoking ; Swine
Chemical Substances	Antioxidants ; Chelating Agents ; Smoke
Language	English
Publishing date	2021-06-10
Publishing country	England
Document type	Journal Article
ZDB-ID	1229160-2
ISSN	1600-0846 ; 0909-752X ; 1397-1344
ISSN (online)	1600-0846
ISSN	0909-752X ; 1397-1344
DOI	10.1111/srt.13068
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Article ; Online: A Dermonutrient Containing Special Collagen Peptides Improves Skin Structure and Function: A Randomized, Placebo-Controlled, Triple-Blind Trial Using Confocal Laser Scanning Microscopy on the Cosmetic Effects and Tolerance of a Drinkable Collagen Supplement.

Laing, Sabrina / Bielfeldt, Stephan / Ehrenberg, Carolin / Wilhelm, Klaus-Peter

Journal of medicinal food

2019 Volume 23, Issue 2, Page(s) 147–152

Abstract: The purpose of this randomized, placebo-controlled, triple-blind trial on 60 healthy female volunteers was to assess the cosmetic effects on skin quality of a food supplement containing special collagen peptides together with acerola extract, vitamin C, ... ...

Abstract	The purpose of this randomized, placebo-controlled, triple-blind trial on 60 healthy female volunteers was to assess the cosmetic effects on skin quality of a food supplement containing special collagen peptides together with acerola extract, vitamin C, vitamin E, biotin, and zinc after an intake of 12 weeks (Elasten
MeSH term(s)	Collagen/pharmacology ; Dietary Supplements ; Double-Blind Method ; Female ; Humans ; Microscopy, Confocal ; Middle Aged ; Skin/drug effects ; Skin Aging/drug effects ; Vitamins/pharmacology
Chemical Substances	Vitamins ; Collagen (9007-34-5)
Language	English
Publishing date	2019-12-27
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	1427365-2
ISSN	1557-7600 ; 1096-620X
ISSN (online)	1557-7600
ISSN	1096-620X
DOI	10.1089/jmf.2019.0197
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Article ; Online: A new dexpanthenol-containing liquid cleanser for atopic-prone skin: Results from two prospective clinical studies evaluating cutaneous tolerability, moisturization potential, and effects on barrier function.

Peltier, Erwan / Trapp, Sonja / de Salvo, Raffaella / Sun, Connie / Brandt, Marianne / Laing, Sabrina / Hennighausen, Natascha / Barrionuevo-Gonzalez, Ana

Journal of cosmetic dermatology

2022 Volume 21, Issue 9, Page(s) 3859–3866

Abstract: Background: Gentle cleansing of the skin without further compromising its barrier function and moisture content and being simultaneously devoid of adverse effects on the skin microbiome are important features of body cleansers for atopic-prone skin ... ...

Abstract	Background: Gentle cleansing of the skin without further compromising its barrier function and moisture content and being simultaneously devoid of adverse effects on the skin microbiome are important features of body cleansers for atopic-prone skin sufferers. For this population, a new dexpanthenol-containing liquid cleanser (DCLC) was developed. Methods: Two prospective 4-week studies have been conducted. Study 1 investigated the effect of once-daily DCLC on stratum corneum (SC) hydration, transepidermal water loss (TEWL), skin pH, and skin microbiome (all on the volar forearm) in adult subjects with dry skin (N = 44). Study 2 explored the cutaneous tolerability of DCLC and its effect on the microbiome biodiversity of the volar forearm skin in infants/children with atopic-prone skin (N = 33, aged 6 months to 6 years). In the latter study, DCLC was applied 2-3 days/week in combination with an emollient applied at least twice daily. Results: In Study 1, on Day 29, the mean change in skin surface capacitance from baseline was significantly greater in the forearm test area treated with DCLC than in the contralateral test area (control) treated with water only (5.16 vs. 3.65 a.u.; p = 0.011), suggesting long-term SC hydration. DCLC use was not associated with changes in TEWL, skin pH, or microbiome biodiversity if compared to control. In Study 2, the 4-week use of DCLC in combination with an emollient was well tolerated according to pediatrician skin assessment, and no flare-ups were recorded. The microbiome biodiversity did not shift during the study. Conclusion: These findings support the use of DCLC in subjects with atopic-prone skin.
MeSH term(s)	Adult ; Child ; Emollients ; Humans ; Infant ; Pantothenic Acid/analogs & derivatives ; Prospective Studies ; Skin ; Water/metabolism ; Water Loss, Insensible
Chemical Substances	Emollients ; Water (059QF0KO0R) ; Pantothenic Acid (19F5HK2737) ; dexpanthenol (1O6C93RI7Z)
Language	English
Publishing date	2022-07-31
Publishing country	England
Document type	Journal Article
ZDB-ID	2280551-5
ISSN	1473-2165 ; 1473-2130
ISSN (online)	1473-2165
ISSN	1473-2130
DOI	10.1111/jocd.15252
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Zs.A 6539: Show issues

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Book ; Online ; Thesis: Die strukturelle und funktionelle Charakterisierung der ADP-Ribosylierung von humanem Tumor-Nekrose-Faktor durch ADP-Ribosyltransferase-1

Laing, Sabrina

2010

Author's details	vorgelegt von Sabrina Laing
Language	German
Size	Online-Ressource
Publisher	Staats- und Universitätsbibliothek Carl von Ossietzky
Publishing place	Hamburg
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Univ., FB Biologie, Diss.--Hamburg, 2010
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article ; Online: Confocal Raman Spectroscopy as a tool to measure the prevention of skin penetration by a specifically designed topical medical device.

Laing, Sabrina / Bielfeldt, Stephan / Wilhelm, Klaus-Peter / Obst, Joachim

2019 Volume 25, Issue 4, Page(s) 578–586

Abstract: Background/aim: The scope of this study was to utilize confocal Raman spectroscopy in the evaluation of the degree of non-penetration into the viable skin layers of a paraffin and petrolatum-based product for use in the intimate areas of the skin. The ... ...

Abstract	Background/aim: The scope of this study was to utilize confocal Raman spectroscopy in the evaluation of the degree of non-penetration into the viable skin layers of a paraffin and petrolatum-based product for use in the intimate areas of the skin. The formulation was purposely designed with properties to prevent undesirable skin penetration. Methods: Product-The test product was a proprietary topical medical device comprising paraffinum liquidum, petrolatum, paraffin, and tocopheryl acetate. Volunteers-A total of 20 healthy volunteers were recruited onto the study-17 females and three males. Product Testing-Raman spectra were obtained at Baseline and 90 minutes after product application. Product Penetration-Skin penetration was calculated from Raman spectra taken at skin depths of -5, 0, 5, 10, 15, and 20 μm. Results: Raman spectra of the investigated product could be clearly differentiated from the skin spectrum. The minimum measurable concentration of the test product was determined at a detection level of 0.5%. In this study, the test product did not penetrate down to skin depths of 10 to 20 μm. Conclusions: Within the precision range of the test method, the investigated product did not penetrate into the compact part of the stratum corneum. The study revealed Raman spectroscopy to be suitable to detect not only penetration but also non-penetration of substances into human skin.
MeSH term(s)	Administration, Topical ; Adult ; Epidermis/metabolism ; Female ; Humans ; Male ; Middle Aged ; Mineral Oil/administration & dosage ; Mineral Oil/metabolism ; Skin/metabolism ; Skin Absorption/physiology ; Spectrum Analysis/instrumentation ; Spectrum Analysis, Raman/methods
Chemical Substances	Mineral Oil (8020-83-5)
Language	English
Publishing date	2019-02-16
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	1229160-2
ISSN	1600-0846 ; 0909-752X ; 1397-1344
ISSN (online)	1600-0846
ISSN	0909-752X ; 1397-1344
DOI	10.1111/srt.12689
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Article: New methods for assessing secondary performance attributes of sunscreens suitable for professional outdoor work.

Rocholl, Marc / Weinert, Patricia / Bielfeldt, Stephan / Laing, Sabrina / Wilhelm, Klaus Peter / Ulrich, Claas / John, Swen Malte

Journal of occupational medicine and toxicology (London, England)

2021 Volume 16, Issue 1, Page(s) 25

Abstract: Background: Outdoor workers (OW) are highly exposed to solar ultraviolet radiation (UVR) and thus at increased risk for developing skin cancer. An essential part of an overall strategy to reduce workplace UVR-exposure to OW's skin is the usage of ... ...

Abstract	Background: Outdoor workers (OW) are highly exposed to solar ultraviolet radiation (UVR) and thus at increased risk for developing skin cancer. An essential part of an overall strategy to reduce workplace UVR-exposure to OW's skin is the usage of sunscreens. However, compliance with regular sunscreen usage seems to be low, as products are usually designed for recreational sun exposure and thus do not meet the requirements of physically active OW. To date, no standardized test procedures assess the suitability of sunscreens for professional use. The aim of this pilot study was to develop standardized methods of testing secondary performance attributes (PA) to represent real-life working conditions of outdoor work. Methods: Ten sunscreen products, carefully selected after a detailed market survey of all relevant producers available on the German market, were evaluated regarding their suitability for professional outdoor work on 24 healthy volunteers in a newly designed test procedure. In addition to three standardized efficacy characteristics, i.e., sun protection factor, water-resistance, and UVA protection, we evaluated each PA involving parameters typically associated with outdoor workplaces. Results: We developed standardized methods for objectifying the suitability of sunscreen products for professional outdoor work. The test procedures used are well feasible and appropriate for testing the PA because they represent practical working conditions in detail - although the degree of discriminability of single test methods varied. The claimed sun protection factor (SPF) of the products was confirmed; bio-stability of the SPF after physical activity was achieved in most cases. While most products hardly irritate the eyes and are quickly absorbed, the evaluation of the subjective skin feeling and non-slip grip is inconsistent. Conclusions: In this pilot study, for the first time secondary PA are defined and examined. Although further objectification of the PA assessment as well as the establishment of minimum standards should be sought, the new methods could already complement the so far mandatory labels and in this way provide a significant impetus for the current scientific and political focus on the improvement of occupational health in highly UVR-exposed OW.
Language	English
Publishing date	2021-07-05
Publishing country	England
Document type	Journal Article
ISSN	1745-6673
ISSN	1745-6673
DOI	10.1186/s12995-021-00314-2
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Article ; Online: Characterization and validation of an in vivo confocal Raman spectroscopy led tri-method approach in the evaluation of the lip barrier.

Bielfeldt, Stephan / Laing, Sabrina / Sadowski, Tomasz / Gunt, Hemali / Wilhelm, Klaus-Peter

2019 Volume 26, Issue 3, Page(s) 390–397

Abstract: Background/aim: It was the aim to establish and validate in vivo confocal Raman spectroscopy for characterization of the lip barrier in conjunction with transepidermal water loss (TEWL) and skin capacitance assessments. For the first time in vivo, ... ...

Abstract	Background/aim: It was the aim to establish and validate in vivo confocal Raman spectroscopy for characterization of the lip barrier in conjunction with transepidermal water loss (TEWL) and skin capacitance assessments. For the first time in vivo, barrier-relevant components of the lip (derived, natural moisturizing factors (NMFs) and ceramides are described. Methods: In 32 healthy volunteers, a dental tongue fixation device was inserted to prevent both voluntary and involuntary lip moisturization during measurements. Seventeen individual parameters relating to water, ceramide, and NMF content were assessed via Raman spectroscopy. Additionally, corneometry and TEWL were measured. To give a guidance for the required volunteer group size of future lip barrier studies for all test parameters, coefficients of variation (CV) were calculated and plots showing the required sample size for a given percentage treatment effect. Results: Raman spectroscopy assessed parameters on the lower lip comprehensively characterized the state of the lip barrier. Parameter variability was sufficiently low to corroborate changes in most parameters using relatively small study populations. Conclusions: Lip skin is comparatively well hydrated. Biophysical measurement of the lip barrier function is a challenge, as unconscious licking of the lower lip has to be prevented. In vivo confocal Raman spectroscopy provides insightful parameters for the characterization of the lip barrier and sufficiently low inter-individual variability to assess relatively small parameter changes employing relatively few study subjects. Differences at the molecular level and at a high spatial resolution are detectable, and these insights might provide a breakthrough in the evaluation of lip barrier function and developing solutions for lip care.
MeSH term(s)	Adult ; Ceramides/chemistry ; Ceramides/metabolism ; Epidermis/metabolism ; Female ; Humans ; Lip/chemistry ; Lip/metabolism ; Middle Aged ; Skin/chemistry ; Skin/metabolism ; Skin Absorption/physiology ; Skin Physiological Phenomena ; Spectrum Analysis, Raman/methods ; Water Loss, Insensible/physiology
Chemical Substances	Ceramides
Language	English
Publishing date	2019-12-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1229160-2
ISSN	1600-0846 ; 0909-752X ; 1397-1344
ISSN (online)	1600-0846
ISSN	0909-752X ; 1397-1344
DOI	10.1111/srt.12814
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Article ; Online: New methods for assessing secondary performance attributes of sunscreens suitable for professional outdoor work

Marc Rocholl / Patricia Weinert / Stephan Bielfeldt / Sabrina Laing / Klaus Peter Wilhelm / Claas Ulrich / Swen Malte John

Journal of Occupational Medicine and Toxicology, Vol 16, Iss 1, Pp 1-

2021 Volume 11

Abstract: Abstract Background Outdoor workers (OW) are highly exposed to solar ultraviolet radiation (UVR) and thus at increased risk for developing skin cancer. An essential part of an overall strategy to reduce workplace UVR-exposure to OW’s skin is the usage of ...

Abstract	Abstract Background Outdoor workers (OW) are highly exposed to solar ultraviolet radiation (UVR) and thus at increased risk for developing skin cancer. An essential part of an overall strategy to reduce workplace UVR-exposure to OW’s skin is the usage of sunscreens. However, compliance with regular sunscreen usage seems to be low, as products are usually designed for recreational sun exposure and thus do not meet the requirements of physically active OW. To date, no standardized test procedures assess the suitability of sunscreens for professional use. The aim of this pilot study was to develop standardized methods of testing secondary performance attributes (PA) to represent real-life working conditions of outdoor work. Methods Ten sunscreen products, carefully selected after a detailed market survey of all relevant producers available on the German market, were evaluated regarding their suitability for professional outdoor work on 24 healthy volunteers in a newly designed test procedure. In addition to three standardized efficacy characteristics, i.e., sun protection factor, water-resistance, and UVA protection, we evaluated each PA involving parameters typically associated with outdoor workplaces. Results We developed standardized methods for objectifying the suitability of sunscreen products for professional outdoor work. The test procedures used are well feasible and appropriate for testing the PA because they represent practical working conditions in detail – although the degree of discriminability of single test methods varied. The claimed sun protection factor (SPF) of the products was confirmed; bio-stability of the SPF after physical activity was achieved in most cases. While most products hardly irritate the eyes and are quickly absorbed, the evaluation of the subjective skin feeling and non-slip grip is inconsistent. Conclusions In this pilot study, for the first time secondary PA are defined and examined. Although further objectification of the PA assessment as well as the establishment of minimum ...
Keywords	Sunscreening agents ; Sun protection factor ; Ultraviolet rays ; Outdoor work ; Photoprotection ; Occupational health ; Industrial medicine. Industrial hygiene ; RC963-969
Subject code	600
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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