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Article ; Online: Abnormalities on chest computed tomography in patients with coronavirus disease 2019.

Rogalska-Płońska, Magdalena / Kuźmicz, Andrzej / Łapiński, Tadeusz W / Flisiak, Robert

2020 Volume 130, Issue 6, Page(s) 541–543

MeSH term(s)	Adult ; Aged ; Betacoronavirus ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/diagnostic imaging ; Humans ; Lung/diagnostic imaging ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/diagnostic imaging ; SARS-CoV-2
Keywords	covid19
Language	English
Publishing date	2020-05-18
Publishing country	Poland
Document type	Case Reports ; Journal Article
ZDB-ID	123500-x
ISSN	1897-9483 ; 0032-3772
ISSN (online)	1897-9483
ISSN	0032-3772
DOI	10.20452/pamw.15363
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Article: Effect of COVID-19 on Anti-S Antibody Response in Healthcare Workers Six Months Post-Vaccination.

Flisiak, Robert / Pawłowska, Małgorzata / Rogalska-Płońska, Magdalena / Bociąga-Jasik, Monika / Kłos, Krzysztof / Piekarska, Anna / Zarębska-Michaluk, Dorota

Vaccines

2021 Volume 9, Issue 11

Abstract: The current study aimed to determine to what extent prior COVID-19 infection affects the response of specific antibodies following vaccination. The study involved 173 healthcare professionals who completed the two-dose vaccination course with BNT162b2, ... ...

Abstract	The current study aimed to determine to what extent prior COVID-19 infection affects the response of specific antibodies following vaccination. The study involved 173 healthcare professionals who completed the two-dose vaccination course with BNT162b2, including 40 who previously experienced clinical COVID-19. The levels of anti-SARS-CoV-2 S1S2 IgG (anti-S) and, in some cases, anti-SARS-CoV-S-RBD IgG (anti-S-RBD) were determined six months after complete vaccination. A level exceeding the cut-off values for both anti-S and anti-S-RBD was observed in 100% of subjects, but after setting the analysis to 5- and 10-fold cut-off levels, the percentage of subjects meeting this criterion was significantly higher for anti-S-RBD. The 100-fold cut-off level was achieved by only 21% and 16% for anti-S and anti-S-RBD, respectively. Anti-S and anti-S-RBD levels above ten times the positive cut-off were respectively observed in 91% and 100% individuals with a history of COVID-19, while among those without COVID-19, these values were 64% and 90%, respectively. Significantly higher incidence of values above 10 and 100 times the cut-off became apparent among people with a history of COVID-19. In conclusion, vaccination against COVID-19 following infection with the disease provides higher levels of specific antibodies 6 months after vaccination than those of individuals without a history of the disease, which supports the use of a booster dose, particularly for those who have not experienced SARS-CoV-2 infection.
Language	English
Publishing date	2021-11-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines9111325
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Article ; Online: Metabolic syndrome in HIV infected adults in Poland.

Rogalska-Płońska, Magdalena / Grzeszczuk, Anna / Rogalski, Paweł / Łucejko, Mariusz / Flisiak, Robert

Kardiologia polska

2018 Volume 76, Issue 3, Page(s) 548–553

Abstract: Background: Metabolic syndrome (MS) is usually diagnosed based on the presence of abdominal obesity, elevated blood pres-sure (BP), elevated fasting plasma glucose, high serum triglycerides (TG), and low high-density lipoprotein (HDL) cholesterol levels. ...

Abstract	Background: Metabolic syndrome (MS) is usually diagnosed based on the presence of abdominal obesity, elevated blood pres-sure (BP), elevated fasting plasma glucose, high serum triglycerides (TG), and low high-density lipoprotein (HDL) cholesterol levels. Whether HIV is associated with a higher prevalence of MS than in the general population remains unclear. Aim: The aim of the study was to determine the incidence of MS in the population of HIV-infected adults and its association with clinical, virological, and biochemical features. Methods: Two hundred and seventy HIV-infected Caucasian adult patients were enrolled in the study and evaluated based on clinical records in the years 2013-2015. Results: Metabolic syndrome was diagnosed in 60 of 270 (22%) patients, 47 (24%) males and 13 (17%) females, mostly (72%) aged above 40 years. The percentage of patients with diagnosed MS in specific age groups in comparison to the general Polish population for females aged < 40 years was 7% vs. 4%, and males in the same age - 18% vs. 9%, for females aged 40-59 years - 47% vs. 24.4%, and males - 33% vs. 28.3%. Particular components of MS in the MS population were found as follows: body mass index > 30 kg/m2 in 29%, waist circumference exceeding 94 cm in men and 80 cm in woman - 87.5%, TG ≥ 150 mg/dL - 82%, HDL cholesterol < 40/50 mg/dL (males/females) - 42%, systolic/diastolic BP ≥ 130 mmHg/≥ 85 mmHg - 83%, and fasting glucose > 100 mg/dL - 42%. In stepwise multivariate logistic regression analysis, age (odds ratio [OR] 1.052, 95% con-fidence interval [CI] 1.018-1.088, p = 0.003) and nadir CD4 < 350 cells/mm3 (OR 3.576, 95% CI 1.035-12.355, p = 0.04) were associated with MS. Patients with MS compared with those without this disorder had low, intermediate, high, and very high cardiovascular risk in 10% vs. 23%, 73% vs. 70%, 7% vs. 5%, and 10% vs. 2%, respectively (p = 0.006). Conclusions: Prevalence of MS in the HIV-infected population is higher than in the general Polish population. Age and low nadir CD4 were found to be associated with MS.
MeSH term(s)	Adolescent ; Adult ; Female ; HIV Infections/complications ; Humans ; Male ; Metabolic Syndrome/complications ; Metabolic Syndrome/epidemiology ; Middle Aged ; Poland/epidemiology ; Prevalence ; Young Adult
Language	English
Publishing date	2018-01-19
Publishing country	Poland
Document type	Journal Article
ZDB-ID	411492-9
ISSN	1897-4279 ; 0022-9032
ISSN (online)	1897-4279
ISSN	0022-9032
DOI	10.5603/KP.a2017.0249
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Article ; Online: Elevated levels of soluble glycoprotein V - The plasma marker of platelet activation by thrombin in patients with early stage primary biliary cholangitis (PBC).

Rogalski, Pawel / Bogdanowska-Charkiewicz, Dagmara / Rogalska-Plonska, Magdalena / Lukaszewicz-Zajac, Marta / Kostecka-Roslen, Ines / Mroczko, Barbara / Dabrowska, Milena / Wasielica-Berger, Justyna / Aleksiejuk, Elzbieta / Antonowicz, Stefania / Dabrowski, Andrzej / Daniluk, Jaroslaw / Janica, Jacek

Advances in medical sciences

2023 Volume 68, Issue 1, Page(s) 71–78

Abstract: Purpose: There is a growing body of evidence for a prothrombotic tendency in patients with primary biliary cholangitis (PBC). The aim of the study was to evaluate coagulation disorders in patients with early stage PBC compared to healthy controls and ... ...

Abstract	Purpose: There is a growing body of evidence for a prothrombotic tendency in patients with primary biliary cholangitis (PBC). The aim of the study was to evaluate coagulation disorders in patients with early stage PBC compared to healthy controls and evaluation of their relationship with clinical data, with particular emphasis on minimal hepatic encephalopathy (MHE). Patients and methods: Fifty-one participants (PBC group - 38 patients, all patients but one Child-Pugh A; control group - 13 healthy controls) were included in our prospective, single center study. We assessed the plasma levels of sGPV, plasma procoagulant phospholipids (PPL) and rotational thromboelastometry (ROTEM) profiles in all study participants. Porto-systemic encephalopathy syndrome test was used to assess MHE. Results: The sGPV levels were higher in the PBC group compared to the controls: 36.07 ± 11.32 ng/mL vs 27.04 ± 11.72 ng/mL, p = 0.031. The PPL level was lower in the PBC group compared to controls resulting in increased clotting time in a factor Xa-based coagulation assay: 54.65 (47.83-58.83) sec. vs 45.90 (43.3-50.5) sec., p = 0.0065. PPL levels were correlated with platelet count (rho = -0.46, p = 0.001). ROTEM parameters did not differ significantly between groups. Coagulation parameters did not differ significantly between patients with and without MHE. Conclusions: We have showed increased levels of sGPV - a plasma marker of platelet activation by thrombin in patients with early stage PBC compared to healthy controls. We found no relationship between the coagulation disorders and the occurrence of MHE. The PPL level was lower in the PBC group.
MeSH term(s)	Humans ; Thrombin ; Liver Cirrhosis, Biliary ; Prospective Studies ; Platelet Activation ; Glycoproteins
Chemical Substances	Thrombin (EC 3.4.21.5) ; Glycoproteins
Language	English
Publishing date	2023-02-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2273668-2
ISSN	1898-4002 ; 1896-1126
ISSN (online)	1898-4002
ISSN	1896-1126
DOI	10.1016/j.advms.2023.01.002
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Article ; Online: Effect of COVID-19 on Anti-S Antibody Response in Healthcare Workers Six Months Post-Vaccination

Robert Flisiak / Małgorzata Pawłowska / Magdalena Rogalska-Płońska / Monika Bociąga-Jasik / Krzysztof Kłos / Anna Piekarska / Dorota Zarębska-Michaluk

Vaccines, Vol 9, Iss 1325, p

2021 Volume 1325

Abstract	The current study aimed to determine to what extent prior COVID-19 infection affects the response of specific antibodies following vaccination. The study involved 173 healthcare professionals who completed the two-dose vaccination course with BNT162b2, including 40 who previously experienced clinical COVID-19. The levels of anti-SARS-CoV-2 S1S2 IgG (anti-S) and, in some cases, anti-SARS-CoV-S-RBD IgG (anti-S-RBD) were determined six months after complete vaccination. A level exceeding the cut-off values for both anti-S and anti-S-RBD was observed in 100% of subjects, but after setting the analysis to 5- and 10-fold cut-off levels, the percentage of subjects meeting this criterion was significantly higher for anti-S-RBD. The 100-fold cut-off level was achieved by only 21% and 16% for anti-S and anti-S-RBD, respectively. Anti-S and anti-S-RBD levels above ten times the positive cut-off were respectively observed in 91% and 100% individuals with a history of COVID-19, while among those without COVID-19, these values were 64% and 90%, respectively. Significantly higher incidence of values above 10 and 100 times the cut-off became apparent among people with a history of COVID-19. In conclusion, vaccination against COVID-19 following infection with the disease provides higher levels of specific antibodies 6 months after vaccination than those of individuals without a history of the disease, which supports the use of a booster dose, particularly for those who have not experienced SARS-CoV-2 infection.
Keywords	COVID-19 ; SARS-CoV-2 ; vaccination ; Medicine ; R
Subject code	360
Language	English
Publishing date	2021-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article: Abnormalities on chest computed tomography in patients with coronavirus disease 2019

Rogalska-Plonska, Magdalena / Kuzmicz, Andrzej / Lapinski, Tadeusz W / Flisiak, Robert

Pol Arch Intern Med

Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #291092
Database	COVID19

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Article ; Online: Minimal hepatic encephalopathy may be present despite the absence of non-invasive and elastography evidence of cirrhosis in patients with primary biliary cholangitis.

Bogdanowska-Charkiewicz, Dagmara / Rogalski, Pawel / Janica, Jacek / Rogalska-Plonska, Magdalena / Aleksiejuk, Elzbieta / Antonowicz, Stefania / Dabrowski, Andrzej / Daniluk, Jarosław

Advances in medical sciences

2021 Volume 66, Issue 2, Page(s) 293–301

Abstract: Purpose: Minimal hepatic encephalopathy (MHE) is an important complication of chronic liver disease (CLD); however, MHE burden in patients with primary biliary cholangitis (PBC) has not been determined yet. Therefore, our study aimed to assess the ... ...

Abstract	Purpose: Minimal hepatic encephalopathy (MHE) is an important complication of chronic liver disease (CLD); however, MHE burden in patients with primary biliary cholangitis (PBC) has not been determined yet. Therefore, our study aimed to assess the prevalence of MHE in a typical cohort of middle-aged, patients with PBC suspicion of liver fibrosis and to investigate the relationship between MHE, basic laboratory tests and the stage of liver fibrosis. Patients and methods: Fifty-one patients (38 with PBC and 13 controls), were prospectively enrolled. Portosystemic Encephalopathy-Syndrome test was used to diagnose MHE. Elastography point qualification (ElastPQ) and non-invasive markers (APRI and FIB-4) were used to assess liver fibrosis. The severity of CLD was assessed using the Model of End-Stage Liver Disease (MELD) and Child-Pugh score. Results: MHE was diagnosed in 9 patients (24.3%) with PBC and none in the control group. As many as 44.4% of the patients with MHE had neither advanced fibrosis nor cirrhosis, as demonstrated using non-invasive markers of liver fibrosis or ElastPQ. The MELD score was the only predictor of MHE with cut-off value 8.5 [AUC = 0.753, CI Conclusions: MHE may occur in PBC despite no evidence of advanced liver fibrosis or cirrhosis. The slightly elevated MELD score may indicate a substantially increased risk of MHE in patients with PBC.
MeSH term(s)	Elasticity Imaging Techniques ; Hepatic Encephalopathy/etiology ; Humans ; Liver Cirrhosis/complications ; Liver Cirrhosis, Biliary/complications ; Middle Aged ; Severity of Illness Index
Language	English
Publishing date	2021-06-27
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2273668-2
ISSN	1898-4002 ; 1896-1126
ISSN (online)	1898-4002
ISSN	1896-1126
DOI	10.1016/j.advms.2021.06.001
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Article: The occurrence of autoantibodies in patients with chronic HCV infection, including patients dialyzed and after kidney transplantation.

Łapiński, Tadeusz W / Rogalska-Płońska, Magdalena / Parfieniuk-Kowerda, Anna / Świderska, Magdalena / Flisiak, Robert

Clinical and experimental hepatology

2016 Volume 2, Issue 4, Page(s) 161–166

Abstract: Introduction: There are reports suggesting that hepatitis C virus (HCV) may stimulate the autoimmune process. Studies have been undertaken to evaluate the occurrence and type of autoantibodies in HCV-infected patients with and without immunosuppression. ...

Abstract	Introduction: There are reports suggesting that hepatitis C virus (HCV) may stimulate the autoimmune process. Studies have been undertaken to evaluate the occurrence and type of autoantibodies in HCV-infected patients with and without immunosuppression. Results were analyzed according to HCV genotype, intensity of inflammation and liver fibrosis stage. Material and methods: The study included 105 patients chronically infected with HCV, including 25 with immunological suppression administered for kidney disease or kidney transplantation. Blood samples were tested by immunoblotting for the presence of AMA-M2, SLA/LP, LKM-1, LC1, anti-F-actin, anti-desmin, anti-myosin, anti-gp210 and anti-sp100 autoantibodies, and ANA. All the patients were scored for autoimmune hepatitis. Results: Autoantibodies were detected in 32.5% of patients without immunosuppression and in 16% with immunosuppression. Single types of autoantibodies were identified in 26% of patients. The most frequent ones were ANA (19%) and AMA-M2 (5.7%). The presence of antibodies in patients with genotype 1 was significantly higher in comparison to their occurrence in genotype 3. Autoimmune hepatitis was not diagnosed in any of the patients. Immunoglobulin G level was significantly higher in patients with detectable autoantibodies, compared to patients without antibodies (1.89 vs. 1.28 g/dl, Conclusions: The antibodies are significantly more frequent in patients without immunosuppression and in patients infected with genotype 1 than genotype 3. The presence of these autoantibodies is not associated with the development of autoimmune hepatitis. Higher level of immunoglobulin G in the serum correlates with the presence of autoantibodies.
Language	English
Publishing date	2016-11-28
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2937940-4
ISSN	2449-8238 ; 2392-1099
ISSN (online)	2449-8238
ISSN	2392-1099
DOI	10.5114/ceh.2016.63874
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Article: Significance of mutations in the region coding for NS3/4 protease in patients infected with HCV genotype 1b.

Łapiński, Tadeusz W / Rogalska-Płońska, Magdalena / Kowalczuk, Oksana / Kiśluk, Joanna / Zurnowska, Joanna / Nikliński, Jacek / Flisiak, Robert

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

2018 Volume 27, Issue 11, Page(s) 1593–1600

Abstract: Background: Fast hepatitis C virus (HCV) replication is one of the reasons for frequent changes in viral genome.: Objectives: The objective of this study was to evaluate the frequency and type of mutation in NS3/4 protease in patients with HCV ... ...

Abstract	Background: Fast hepatitis C virus (HCV) replication is one of the reasons for frequent changes in viral genome. Objectives: The objective of this study was to evaluate the frequency and type of mutation in NS3/4 protease in patients with HCV genotype 1b and to determine the effect of the mutation on viral load, fibrosis stage, alanine aminotransferase (ALT) activity, and alpha-fetoprotein (AFP) level. Material and methods: The study included 46 treatment-naïve patients, infected with HCV genotype 1b. Mutations were analyzed after isolating HCV RNA, and then evaluating the compliance of the amino acid sequence, using 3500 Genetic Analyzer (Applied Biosystems, Foster City, USA). RNA fragment from nucleotide 1-181 encoding NS3/4 protease was subjected to analysis. Results: Mutations were demonstrated in 65% of subjects. Changes in the protease region affecting resistance to treatment (T54, Q80, V158, M175, D186) were detected in 10.8% of patients. Substitution mutation at T72 was found most frequently - in 49.9% of cases. In 13% of patients, mutation at G86 was demonstrated, including G86P in 5 patients and G86S in 1 patient. In the group of patients with T72 mutation, viral load was significantly higher (1.3 × 106 IU/mL vs 1.0 × 105 IU/mL; p = 0.01), AFP level was higher and fibrosis level was lower (1.26 vs 2.17; p = 0.008) compared to the patients without the mutation. Cryoglobulinemia was observed in 74% of patients with mutation at position T72. Conclusions: Natural mutations of the region coding for NS3/4 protease are found frequently in patients infected with genotype 1b, but they may cause resistance to antiviral agents only in 11% of patients. Changes were most frequently found at position T72. Mutations at position T72 are correlated with the cryoglobulinemia occurrence. This is a substitution mutation, accompanied by a high viral load, high ALT activity and AFP level, which may point to a more unfavorable influence of such a modified virus, compared to wild-type virus, onto pathological processes in the liver.
MeSH term(s)	Amino Acid Sequence ; Antiviral Agents/therapeutic use ; Cryoglobulinemia/virology ; Drug Resistance, Viral/genetics ; Genotype ; Hepacivirus/genetics ; Hepacivirus/isolation & purification ; Hepatitis C, Chronic/diagnosis ; Hepatitis C, Chronic/drug therapy ; Hepatitis C, Chronic/genetics ; Humans ; Mutation ; Peptide Hydrolases ; Viral Load ; Viral Nonstructural Proteins/genetics
Chemical Substances	Antiviral Agents ; NS3 protein, hepatitis C virus ; NS4 protein, hepatitis C virus ; Viral Nonstructural Proteins ; Peptide Hydrolases (EC 3.4.-)
Language	English
Publishing date	2018-07-25
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2270257-X
ISSN	1899-5276 ; 1230-025X
ISSN	1899-5276 ; 1230-025X
DOI	10.17219/acem/75511
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Article ; Online: Serum Cytokeratin 18 M30 Levels in Chronic Hepatitis B Reflect Both Phase and Histological Activities of Disease.

Świderska, Magdalena / Jaroszewicz, Jerzy / Parfieniuk-Kowerda, Anna / Rogalska-Płońska, Magdalena / Stawicka, Agnieszka / Panasiuk, Anatol / Flisiak, Robert

Mediators of inflammation

2017 Volume 2017, Page(s) 3480234

Abstract: Chronic hepatitis B has highly a dynamic course with significant fluctuations of HBV-DNA and ALT impeding assessment of disease activity. New biomarkers of inflammatory versus noninflammatory stages of HBV infection are urgently needed. Cytokeratin 18 ... ...

Abstract	Chronic hepatitis B has highly a dynamic course with significant fluctuations of HBV-DNA and ALT impeding assessment of disease activity. New biomarkers of inflammatory versus noninflammatory stages of HBV infection are urgently needed. Cytokeratin 18 epitope M30 (M30 CK-18) is a sensitive marker of cell death. We aimed to investigate an association between serum M30 CK-18 and histological activity and phase of HBV infection. 150 Caucasian patients with HBV-infection were included in the study. Serum M30 CK-18 levels reflected phase of disease, being significantly higher in both HBeAg(+) and HBeAg(-) hepatitis B in comparison to HBsAg(+) carrier groups. The highest serum M30 CK-18 levels were observed in subjects with the most advanced stages of HBV. Moreover, its serum concentrations correlated with both inflammatory activity and fibrosis advancement (ANOVA
MeSH term(s)	Adult ; Biomarkers/blood ; Biomarkers/metabolism ; Female ; Hepatitis B e Antigens/genetics ; Hepatitis B e Antigens/metabolism ; Hepatitis B, Chronic/blood ; Humans ; Inflammation/blood ; Keratin-18/blood ; Liver/metabolism ; Male ; Peptide Fragments/blood ; Young Adult
Chemical Substances	Biomarkers ; Hepatitis B e Antigens ; Keratin-18 ; M30 cytokeratin-18 peptide, human ; Peptide Fragments
Language	English
Publishing date	2017-07-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	1137605-3
ISSN	1466-1861 ; 0962-9351
ISSN (online)	1466-1861
ISSN	0962-9351
DOI	10.1155/2017/3480234
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