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Article ; Online: Rapid AST: Possibility of inferring resistance mechanisms with complex phenotypes.

Ligero-López, J / Falces-Romero, I / Aranda-Díaz, A / García-Ballesteros, D / García-Rodríguez, J / Cendejas-Bueno, E

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2023 Volume 37, Issue 1, Page(s) 88–92

Abstract: The new automated systems designed for rapid performance of AST have significantly reduced the response time for susceptibility testing of microorganisms causing bacteremia and sepsis. The Accelerate Pheno® system (AAC) is one such system. Our objective ... ...

Abstract	The new automated systems designed for rapid performance of AST have significantly reduced the response time for susceptibility testing of microorganisms causing bacteremia and sepsis. The Accelerate Pheno® system (AAC) is one such system. Our objective for this study was to determine whether the AAC system is capable of providing an accurate susceptibility profile to infer resistance mechanisms in different carbapenemase-producing isolates when compared to the MicroScan WalkAway System (MWS). Disk diffusion method was also performed on all isolates as a reference method. Additionally, we compared the results obtained with the routine AST production system. We selected 19 isolates from the cryobank of the Microbiology department, all of which were carbapenemase-producing gram-negative bacilli. AAC was able to identify and infer the resistance of a total of 10 isolates, with an EA and CA of 84.2% for meropenem and 88.2% and 64.7% for ertapenem EA and CA, respectively. If we consider the disk diffusion technique, the CA was 57.9% and 76.5% for meropenem and ertapenem. However, in the presence of carbapenemases, AAC was not able to provide adequate MICs or infer the resistance mechanisms of the isolates accurately. Further studies with a larger number of isolates, including the new antibiotics ceftolozane/tazobactam and ceftazidime/avibactam, are needed for a more comprehensive comparison.
MeSH term(s)	Humans ; Meropenem ; Ertapenem ; Anti-Bacterial Agents/pharmacology ; Gram-Negative Bacteria ; beta-Lactamases/genetics ; Microbial Sensitivity Tests ; Pseudomonas aeruginosa
Chemical Substances	Meropenem (FV9J3JU8B1) ; Ertapenem (G32F6EID2H) ; Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2023-11-08
Publishing country	Spain
Document type	Journal Article
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
DOI	10.37201/req/043.2023
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Article ; Online: Expert Consensus on Morphofunctional Assessment in Disease-Related Malnutrition. Grade Review and Delphi Study.

García-Almeida, José Manuel / García-García, Cristina / Ballesteros-Pomar, María D / Olveira, Gabriel / Lopez-Gomez, Juan J / Bellido, Virginia / Bretón Lesmes, Irene / Burgos, Rosa / Sanz-Paris, Alejandro / Matia-Martin, Pilar / Botella Romero, Francisco / Ocon Breton, Julia / Zugasti Murillo, Ana / Bellido, Diego

Nutrients

2023 Volume 15, Issue 3

Abstract: Disease-related malnutrition (DRM) affects approximately a third of hospitalized patients and is associated with an increased risk of morbimortality. However, DRM is often underdiagnosed and undertreated. Our aim is to evaluate the prognostic value of ... ...

Abstract	Disease-related malnutrition (DRM) affects approximately a third of hospitalized patients and is associated with an increased risk of morbimortality. However, DRM is often underdiagnosed and undertreated. Our aim is to evaluate the prognostic value of morphofunctional tools and tests for nutritional assessment in clinical practice. A systematic literature review was conducted to identify studies relating to the morphofunctional assessment of nutritional status and mortality or complications. Evidence was evaluated using the GRADE (Grading of Recommendations, Assessment, Development, and Evaluations) method. Twelve GRADE recommendations were made and divided into seven topics: food intake and nutrient assimilation, anthropometry, biochemical analysis, hand grip strength, phase angle, muscle imaging, and functional status and quality of life. From these recommendations, 37 statements were developed and scored in a two-survey Delphi method by 183 experts. A consensus was reached on accepting 26/37 statements. Surveys had high internal consistency and high inter-rater reliability. In conclusion, evidence-based recommendations were made on the prognostic value of morphofunctional assessment tools and tests to assess malnutrition, most of which were found to be feasible in routine clinical practice, according to expert opinions.
MeSH term(s)	Humans ; Hand Strength ; Consensus ; Delphi Technique ; Quality of Life ; Reproducibility of Results ; Malnutrition/complications ; Malnutrition/diagnosis
Language	English
Publishing date	2023-01-25
Publishing country	Switzerland
Document type	Systematic Review ; Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu15030612
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Article ; Online: Nutritional ultrasound®: Conceptualisation, technical considerations and standardisation.

García-Almeida, José Manuel / García-García, Cristina / Vegas-Aguilar, Isabel María / Ballesteros Pomar, María D / Cornejo-Pareja, Isabel María / Fernández Medina, Beatriz / de Luis Román, Daniel A / Bellido Guerrero, Diego / Bretón Lesmes, Irene / Tinahones Madueño, Francisco J

Endocrinologia, diabetes y nutricion

2023 Volume 70 Suppl 1, Page(s) 74–84

Abstract: Nutritional ultrasound® is a new concept that uses ultrasound to assess body composition. It is composed of the evaluation of fat-free mass and fat mass. It is an emerging, economical, portable, non-invasive technique that evaluates the musculoskeletal ... ...

Abstract	Nutritional ultrasound® is a new concept that uses ultrasound to assess body composition. It is composed of the evaluation of fat-free mass and fat mass. It is an emerging, economical, portable, non-invasive technique that evaluates the musculoskeletal area with linear, broadband, multifrequency probes, with a depth field of 20-100mm. It quantifies muscle modifications in malnutrition and provides information on functional changes (echogenicity). Although there are no validated specific cut-off points, the anterior rectum area of the quadriceps can be used as a criterion for malnutrition. The distribution of adipose tissue provides information on the energy reserve and the inflammatory pattern. It is important to integrate nutritional ultrasound® measures in clinical practice adapted to different settings and pathologies. It is necessary to establish training plans in nutritional ultrasound® for use by Endocrinology and Nutrition Specialists, with the aim of improving the diagnosis and treatment of their patients.
MeSH term(s)	Humans ; Concept Formation ; Body Composition ; Nutritional Status ; Malnutrition ; Ultrasonography
Language	English
Publishing date	2023-03-01
Publishing country	Spain
Document type	Journal Article ; Review
ISSN	2530-0180
ISSN (online)	2530-0180
DOI	10.1016/j.endien.2022.11.010
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Article ; Online: Thyroid panel reference intervals in healthy children and adolescents: A Spanish cohort.

Argente Del Castillo, Paula / Pastor García, Maria I / Morell-Garcia, Daniel / Martinez-Gomez, Laura / Ballesteros, Maria A / Barcelo, Antonia

Clinical biochemistry

2021 Volume 91, Page(s) 39–44

Abstract: Objectives: In children, thyroid hormones are essential for correct physical and neurological development. The recommended process for defining reference intervals (RIs) is the direct approach; however, indirect methods are an effective alternative. ... ...

Abstract	Objectives: In children, thyroid hormones are essential for correct physical and neurological development. The recommended process for defining reference intervals (RIs) is the direct approach; however, indirect methods are an effective alternative. This study aimed to explore age- and sex-dependent relationships between serum concentrations of thyroid hormones in a large population-based cohort of healthy Spanish Caucasian children and calculate RIs. Material and methods: Results of serum thyroid-stimulating hormone (TSH) and free thyroxine (fT4) were collected from laboratory data of N (TSH = 23201; fT4 = 20728) patients aged 1 month - 15 years. These results were validated with a prospective study. Analyses of serum concentrations of TSH and fT4 were performed on ARCHITECT i2000 (Abbott Diagnostics, US). Percentiles (2.5th to 97.5th) were determined for each variable and taken as the RI. Results: No difference was found between serum TSH concentrations in male and female children of all age groups. A difference between serum fT4 concentrations in males and females and an age-dependent correlation for both sexes were found. Conclusion: There is very little consensus on RIs in children. Our data confirm it is possible to use data mining techniques to calculate reliable and clinically useful RIs.
MeSH term(s)	Adolescent ; Age Factors ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Prospective Studies ; Sex Factors ; Spain ; Thyroid Function Tests ; Thyroid Gland ; Thyroid Hormones/blood
Chemical Substances	Thyroid Hormones
Language	English
Publishing date	2021-02-02
Publishing country	United States
Document type	Clinical Trial ; Journal Article ; Multicenter Study
ZDB-ID	390372-2
ISSN	1873-2933 ; 0009-9120
ISSN (online)	1873-2933
ISSN	0009-9120
DOI	10.1016/j.clinbiochem.2021.01.011
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Article ; Online: Evaluation of a lateral flow immunoassay to detect CTX-M extended-spectrum β-lactamases (ESBL) directly from positive blood cultures for its potential use in Antimicrobial Stewardship programs.

Cendejas-Bueno, E / Romero-Gómez, M P / Falces-Romero, I / Aranda-Diaz, A / García-Ballesteros, D / Mingorance, J / García-Rodríguez, J

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2022 Volume 35, Issue 3, Page(s) 284–287

Abstract: Objective: Bloodstream infections (BSI) caused by extended-spectrum beta-lactamases Enterobacteriaceae (ESBL-E) are associated with high rates of treatment failure and increased mortality, especially when appropriate antimicrobial therapy is delayed. ... ...

Abstract	Objective: Bloodstream infections (BSI) caused by extended-spectrum beta-lactamases Enterobacteriaceae (ESBL-E) are associated with high rates of treatment failure and increased mortality, especially when appropriate antimicrobial therapy is delayed. Our aim was to evaluate the anticipation of ESBLs detection and the potential improvement of the time response of the Vitek 2 System (BioMérieux; France). Methods: We compared this lateral flow immunoassay when used directly on fluid from positive blood cultures to the VITEK2 AST system. We evaluated 80 isolates, 61 tested directly on fluid from positive blood cultures and 19 tested on fluid from blood cultures spiked with known ESBL positive and negative organisms. Results: The concordance between the CTX-LFIA and the reference method (Vitek 2) had a Cohen´s Kappa coefficient of 0.97, indicating a particularly good correlation between both compared methods. Conclusions: This lateral flow immunoassay can be more rapid than the Vitek 2 for earlier presumptive identification of CTX-M ESBLs and can be useful to anticipate results and the adjustment of antimicrobial therapy.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Antimicrobial Stewardship ; Blood Culture ; Enterobacteriaceae Infections/drug therapy ; Humans ; Immunoassay ; Microbial Sensitivity Tests ; beta-Lactamases
Chemical Substances	Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2022-03-31
Publishing country	Spain
Document type	Journal Article
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
DOI	10.37201/req/164.2021
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Article ; Online: Interpreting Molecular and Isotopic Biosignatures in Methane-Derived Authigenic Carbonates in the Light of a Potential Carbon Cycle in the Icy Moons.

Carrizo, D / de Dios-Cubillas, A / Sánchez-García, L / López, I / Prieto-Ballesteros, O

Astrobiology

2022 Volume 22, Issue 5, Page(s) 552–567

Abstract: Finding evidence of life beyond Earth is the aim of future space missions to icy moons. Icy worlds with an ocean underlying the icy crust and in contact with a rocky subsurface have great astrobiological interest due to the potential for water-rock ... ...

Abstract	Finding evidence of life beyond Earth is the aim of future space missions to icy moons. Icy worlds with an ocean underlying the icy crust and in contact with a rocky subsurface have great astrobiological interest due to the potential for water-rock interactions that may provide a source of nutrients necessary to sustain life. Such water-rock interactions in icy moons can be indirectly investigated using analogous environments on the deep seafloor on Earth. Here, we investigate the presence of molecular and isotopic biomarkers in two submarine cold seep systems with intense rock-fluid interactions and carbon sink as carbonates with the aim of gaining understanding of potential carbon cycles in the icy worlds' oceans. Authigenic carbonates associated to cold seeps (a chimney from the Gulf of Cádiz and a clathrite from the Pacific Hydrate Ridge) were investigated for their mineralogical composition and lipid biomarker distribution. Molecular and compound-specific isotopic composition of lipid biomarkers allowed us to infer different carbonate origins in both carbonate scenarios: biogenic methane (clathrite) versus thermogenic methane together with allochthonous carbon (chimney). In the Pacific cold seep, carbonate precipitation of the clathrite was deduced to result from the anaerobic oxidation of methane by syntrophic action of methanotrophic archaea with sulfate-reducing bacteria. The distinct carbon sources (thermogenic methane, pelagic biomass, etc.) and sinks (gas clathrates, clathrite, chimney carbonates) were discussed in the light of potentially similar carbon cycling pathways in analogous icy-moon oceans. We show how the isotopic analysis of carbon may be crucial for detecting biosignatures in icy-world carbon sinks. These considerations may affect the strategy of searching for biosignatures in future space missions to the icy worlds.
MeSH term(s)	Archaea/metabolism ; Biomarkers/metabolism ; Carbon ; Carbon Cycle ; Carbonates ; Geologic Sediments/microbiology ; Lipids ; Methane/metabolism ; Moon ; Oxidation-Reduction ; Water
Chemical Substances	Biomarkers ; Carbonates ; Lipids ; Water (059QF0KO0R) ; Carbon (7440-44-0) ; Methane (OP0UW79H66)
Language	English
Publishing date	2022-03-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2047736-3
ISSN	1557-8070 ; 1531-1074
ISSN (online)	1557-8070
ISSN	1531-1074
DOI	10.1089/ast.2021.0036
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Article: Effectiveness and Safety of an Outpatient Program for Percutaneous Left Atrial Appendage Occlusion.

Blanco-Fernández, Fabián / Antúnez-Muiños, Pablo J / Núñez-García, Jean C / López-Tejero, Sergio / Barreira-de Sousa, Gilles J / García-Monsalvo, Mónica / Antúnez-Ballesteros, Milena / Maree, Andrew / González-Calle, David / Rodríguez-Collado, Javier / Barreiro-Pérez, Manuel / Díaz-Peláez, Elena / Pérez Del Villar-Moro, María C / Sánchez-Fernández, Pedro L / Cruz-González, Ignacio

Journal of clinical medicine

2023 Volume 12, Issue 21

Abstract: Background: Left atrial appendage occlusion (LAAO) is a safe and effective alternative to oral anticoagulation for thromboprophylaxis in patients with nonvalvular atrial fibrillation. Technological development in devices and imaging techniques, as well ... ...

Abstract	Background: Left atrial appendage occlusion (LAAO) is a safe and effective alternative to oral anticoagulation for thromboprophylaxis in patients with nonvalvular atrial fibrillation. Technological development in devices and imaging techniques, as well as accumulated experience, have increased procedural success rates and decreased complications. Same-day discharge protocols have been proposed in the field of structural heart disease, but this approach has not been studied in detail for the LAAO procedure. Aim: The aim of this study is to assess the safety and efficacy of an outpatient program for LAAO when compared to the conventional treatment approach. Methods: We present a retrospective, non-randomized single-center study of 262 consecutive patients undergoing LAAO. Patients were divided into two groups, the first ( Results: The overall success rate was 99.6%, with a periprocedural complication rate of 2.29%. With regards to the CP versus OP group, there were no differences between incidences of the primary composite endpoint (6.1% PC vs. 3.0% PA, Conclusions: A same-day discharge LAAO program is safe, effective, and feasible when compared to the conventional strategy. Moreover, it reduces hospital length of stay, which might have clinical and economic benefits.
Language	English
Publishing date	2023-10-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm12216728
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Article ; Online: Urine biomarkers for the prediction of mortality in COVID-19 hospitalized patients.

Morell-Garcia, Daniel / Ramos-Chavarino, David / Bauça, Josep M / Argente Del Castillo, Paula / Ballesteros-Vizoso, Maria Antonieta / García de Guadiana-Romualdo, Luis / Gómez-Cobo, Cristina / Pou, J Albert / Amezaga-Menéndez, Rocío / Alonso-Fernández, Alberto / Llompart, Isabel / García-Raja, Ana

Scientific reports

2021 Volume 11, Issue 1, Page(s) 11134

Abstract: Risk factors associated with severity and mortality attributable to COVID-19 have been reported in different cohorts, highlighting the occurrence of acute kidney injury (AKI) in 25% of them. Among other, SARS-CoV-2 targets renal tubular cells and can ... ...

Abstract	Risk factors associated with severity and mortality attributable to COVID-19 have been reported in different cohorts, highlighting the occurrence of acute kidney injury (AKI) in 25% of them. Among other, SARS-CoV-2 targets renal tubular cells and can cause acute renal damage. The aim of the present study was to evaluate the usefulness of urinary parameters in predicting intensive care unit (ICU) admission, mortality and development of AKI in hospitalized patients with COVID-19. Retrospective observational study, in a tertiary care hospital, between March 1st and April 19th, 2020. We recruited adult patients admitted consecutively and positive for SARS-CoV-2. Urinary and serum biomarkers were correlated with clinical outcomes (AKI, ICU admission, hospital discharge and in-hospital mortality) and evaluated using a logistic regression model and ROC curves. A total of 199 COVID-19 hospitalized patients were included. In AKI, the logistic regression model with a highest area under the curve (AUC) was reached by the combination of urine blood and previous chronic kidney disease, with an AUC of 0.676 (95%CI 0.512-0.840; p = 0.023); urine specific weight, sodium and albumin in serum, with an AUC of 0.837 (95% CI 0.766-0.909; p < 0.001) for ICU admission; and age, urine blood and lactate dehydrogenase levels in serum, with an AUC of 0.923 (95%CI 0.866-0.979; p < 0.001) for mortality prediction. For hospitalized patients with COVID-19, renal involvement and early alterations of urinary and serum parameters are useful as prognostic factors of AKI, the need for ICU admission and death.
MeSH term(s)	Acute Kidney Injury/complications ; Acute Kidney Injury/mortality ; Acute Kidney Injury/physiopathology ; Acute Kidney Injury/urine ; Adult ; Aged ; Area Under Curve ; Biomarkers/urine ; COVID-19/complications ; COVID-19/mortality ; COVID-19/physiopathology ; COVID-19/urine ; Critical Care ; Female ; Hospitalization ; Humans ; Logistic Models ; Male ; Middle Aged ; Observational Studies as Topic ; Prognosis ; ROC Curve ; Retrospective Studies ; Risk Factors ; Severity of Illness Index ; Urine/chemistry
Chemical Substances	Biomarkers
Language	English
Publishing date	2021-05-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-90610-y
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Article ; Online: Quality control of ultrasonography markers for Down's syndrome screening: a retrospective study by the laboratory.

Badal, Blanca / Ballesteros, Antonieta / Crespo, Miriam / Morell-Garcia, Daniel / Bauçà, Josep Miquel / Pastor, Maria / Ruiz de Gopegui, Rosa / Martín, Inmaculada

Diagnosis (Berlin, Germany)

2021

Abstract: Objectives: Quality control of ultrasonography markers is necessary to ensure greater efficacy of prenatal aneuploidy screening. The aim of this study was to assess the quality of the crown-rump length (CRL) and nuchal translucence (NT) measurement ... ...

Abstract	Objectives: Quality control of ultrasonography markers is necessary to ensure greater efficacy of prenatal aneuploidy screening. The aim of this study was to assess the quality of the crown-rump length (CRL) and nuchal translucence (NT) measurement accuracy by the laboratory according to quality indicators. Methods: Retrospective observational study on 4,908 single-foetus pregnant women who underwent prenatal aneuploidy screening in the first trimester of pregnancy. Euploid foetuses with CRL between 45 and 84 mm were included, while those with NT≥3.5 mm were excluded. CRL measurement was considered to be accurate if the median multiples of the median (MoM) for pregnancy-associated plasma protein A (PAPP-A) was between 0.90 and 1.10. Fifteen sonographers participated in the study, six of whom comprised the control group. Systematic error for a sonographer was considered when CRL measurement was greater than ±2 mm with respect to the control group. Quality for NT was assured by means of the WHIRI method and each sonographer Results: For CRL accuracy, five sonographers underestimated the measurements, while another four overestimated them, with no statistical differences. For smaller sized foetuses, all sonographers met the established specifications. Regarding NT control, three sonographers did not meet the quality criteria for the median MoM. All sonographers met the specifications for the logarithmic standard deviation of the NT MoM levels. Thirteen sonographers met the CUSUM specifications. Conclusions: Evaluation of a quality control of ultrasonography parameters by laboratory professionals is necessary to avoid under- or overestimation tendencies for CRL and NT measurements. CUSUM is a useful tool for the immediate correction of errors in NT measurements.
Language	English
Publishing date	2021-06-04
Publishing country	Germany
Document type	Journal Article
ISSN	2194-802X
ISSN (online)	2194-802X
DOI	10.1515/dx-2021-0007
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Article ; Online: Impact on the time elapsed since SARS-CoV-2 infection, vaccination history, and number of doses, on protection against reinfection.

Sánchez-de Prada, Laura / Martínez-García, Ana María / González-Fernández, Belén / Gutiérrez-Ballesteros, Javier / Rojo-Rello, Silvia / Garcinuño-Pérez, Sonsoles / Álvaro-Meca, Alejandro / Ortiz De Lejarazu, Raúl / Sanz-Muñoz, Iván / Eiros, José M

Scientific reports

2024 Volume 14, Issue 1, Page(s) 353

Abstract: SARS-CoV-2 reinfections have been frequent, even among those vaccinated. The aim of this study is to know if hybrid immunity (infection + vaccination) is affected by the moment of vaccination and number of doses received. We conducted a retrospective ... ...

Abstract	SARS-CoV-2 reinfections have been frequent, even among those vaccinated. The aim of this study is to know if hybrid immunity (infection + vaccination) is affected by the moment of vaccination and number of doses received. We conducted a retrospective study in 746 patients with a history of COVID-19 reinfection and recovered the dates of infection and reinfection and vaccination status (date and number of doses). To assess differences in the time to reinfection(t
MeSH term(s)	Humans ; COVID-19/epidemiology ; COVID-19/prevention & control ; SARS-CoV-2 ; Reinfection/epidemiology ; Reinfection/prevention & control ; Retrospective Studies ; Vaccination
Language	English
Publishing date	2024-01-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-50335-6
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