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  1. Article: A

    Imhof, Dennis / Pownall, William Robert / Monney, Camille / Oevermann, Anna / Hemphill, Andrew

    Vaccines

    2021  Volume 9, Issue 12

    Abstract: The apicomplexan ... ...

    Abstract The apicomplexan parasite
    Language English
    Publishing date 2021-11-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9121400
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Thesis: Continuous infusion of escalated amphotericin B deoxycholate

    Imhof, Alexander

    an open-label observational study

    2002  

    Author's details vorgelegt von Alexander Imhof
    Language English
    Size 22 Bl.
    Publishing country Switzerland
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Zürich, Univ., Diss., 2002
    HBZ-ID HT013535131
    Database Catalogue ZB MED Medicine, Health

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  3. Article ; Online: Design, synthesis, and analysis of macrobicyclic peptides for targeting the Gαi protein.

    Pepanian, Anna / Binbay, F Ayberk / Pei, Dehua / Imhof, Diana

    Journal of peptide science : an official publication of the European Peptide Society

    2024  , Page(s) e3565

    Abstract: Bicyclic peptides are important chemical tools that can function, for example, as bioactive ligands switching on/off signaling pathways mediated by guanine nucleotide-binding proteins as bicycles are more broadly applicable. Despite their relevance in ... ...

    Abstract Bicyclic peptides are important chemical tools that can function, for example, as bioactive ligands switching on/off signaling pathways mediated by guanine nucleotide-binding proteins as bicycles are more broadly applicable. Despite their relevance in medicinal chemistry, the synthesis of such peptides is challenging, and the final yield is highly dependent on the chemical composition and physicochemical properties of the scaffold. We recently discovered novel, state-specific peptide modulators targeting the Gαi protein, namely, GPM-2/GPM-3, by screening a one-bead-two-compound combinatorial library. A more detailed analysis, including sequence alignments and computer-assisted conformational studies based on the hit compounds, revealed the new peptide 10 as a potential macrobicyclic Gαi ligand sharing high sequence similarity to the known Gαi modulators. The Gαs protein was included in this study for comparison and to unravel the criteria for the specificity of modulator binding to Gαi versus Gαs. This work provides in-depth computer-assisted experimental studies for the analysis of novel macrobicyclic, library-derived Gαi protein ligands. The sequence and structural comparison of 10 with the lead compounds GPM-2 and GPM-3 reveals the importance of the size and amino acid composition of one ring of the bicyclic system and suggests features enhancing the binding affinity of the peptides to the Gαi protein.
    Language English
    Publishing date 2024-01-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 1234416-3
    ISSN 1099-1387 ; 1075-2617
    ISSN (online) 1099-1387
    ISSN 1075-2617
    DOI 10.1002/psc.3565
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  4. Article: BeiGene - ein neues Sektion-C-Mitglied stellt sich vor. BeiGene - grenzenlos gegen Krebs

    Imhof, A. / Rieger, K.

    Forum

    2023  Volume 38, Issue 3, Page(s) 248

    Language German
    Document type Article
    ZDB-ID 1218650-8
    ISSN 0947-0255
    Database Current Contents Medicine

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  5. Article: Discovery of Native Protein Complexes by Liquid Chromatography Followed by Quantitative Mass Spectrometry.

    Aftab, Wasim / Imhof, Axel

    Advances in experimental medicine and biology

    2021  Volume 1336, Page(s) 105–128

    Abstract: Discovering protein complexes in vivo is of vital importance to understand the evolution and function of biological systems. Proteomics analysis has evolved as a state-of-the-art technique in elucidating the above information. A combination of liquid ... ...

    Abstract Discovering protein complexes in vivo is of vital importance to understand the evolution and function of biological systems. Proteomics analysis has evolved as a state-of-the-art technique in elucidating the above information. A combination of liquid chromatography (LC) and liquid chromatography coupled to shotgun mass spectrometry (LC-MS) provides the most exhaustive information in this regard. However, a significant amount of computational effort is required for the meaningful interpretation of the generated datasets. In this chapter we describe in detail the state-of-the-art pipeline to discover soluble protein complexes and provide practical advice focusing on typical situations a biologist faces while analyzing such proteomics datasets. Furthermore, we briefly describe two commonly used software packages to solve the described problem: Weka for training protein-protein interactions (PPIs) using machine learning (ML) and Cytoscape for clustering the interaction network.
    MeSH term(s) Chromatography, Liquid ; Mass Spectrometry ; Proteomics
    Language English
    Publishing date 2021-10-09
    Publishing country United States
    Document type Journal Article
    ISSN 2214-8019 ; 0065-2598
    ISSN (online) 2214-8019
    ISSN 0065-2598
    DOI 10.1007/978-3-030-77252-9_6
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  6. Book ; Thesis: Plasmaviskosität - Determinanten und regionale Unterschiede

    Imhof, Armin

    Vergleich zweier bevölkerungsbezogener Stichproben mit hohem und mittlerem kardiovaskulärem Risiko

    1998  

    Author's details vorgelegt von Armin Imhof
    Language German
    Size 121 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Ulm, Univ., Diss., 1999
    HBZ-ID HT011219292
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    2000 D 620 order for loan Magazin

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  7. Article ; Online: Hidradenitis suppurativa in transgender and gender diverse patients: A retrospective review with examination of the role of hormone therapy.

    Imhof, Reese L / Todd, Austin / Davidge-Pitts, Caroline J / Alavi, Afsaneh

    Journal of the American Academy of Dermatology

    2023  Volume 90, Issue 4, Page(s) 845–847

    MeSH term(s) Humans ; Hidradenitis Suppurativa/diagnosis ; Retrospective Studies ; Transgender Persons ; Transsexualism ; Hormones
    Chemical Substances Hormones
    Language English
    Publishing date 2023-12-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 603641-7
    ISSN 1097-6787 ; 0190-9622
    ISSN (online) 1097-6787
    ISSN 0190-9622
    DOI 10.1016/j.jaad.2023.12.009
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  8. Article ; Online: In Silico Study of Camptothecin-Based Pro-Drugs Binding to Human Carboxylesterase 2.

    Beierlein, Frank / Horn, Anselm H C / Sticht, Heinrich / Mokhir, Andriy / Imhof, Petra

    Biomolecules

    2024  Volume 14, Issue 2

    Abstract: Pro-drugs, which ideally release their active compound only at the site of action, i.e., in a cancer cell, are a promising approach towards an increased specificity and hence reduced side effects in chemotherapy. A popular form of pro-drugs is esters, ... ...

    Abstract Pro-drugs, which ideally release their active compound only at the site of action, i.e., in a cancer cell, are a promising approach towards an increased specificity and hence reduced side effects in chemotherapy. A popular form of pro-drugs is esters, which are activated upon their hydrolysis. Since carboxylesterases that catalyse such a hydrolysis reaction are also abundant in normal tissue, it is of great interest whether a putative pro-drug is a probable substrate of such an enzyme and hence bears the danger of being activated not just in the target environment, i.e., in cancer cells. In this work, we study the binding mode of carboxylesters of the drug molecule camptothecin, which is an inhibitor of topoisomerase I, of varying size to human carboxylesterase 2 (HCE2) by molecular docking and molecular dynamics simulations. A comparison to irinotecan, known to be a substrate of HCE2, shows that all three pro-drugs analysed in this work can bind to the HCE2 protein, but not in a pose that is well suited for subsequent hydrolysis. Our data suggest, moreover, that for the irinotecan substrate, a reactant-competent pose is stabilised once the initial proton transfer from the putative nucleophile Ser202 to the His431 of the catalytic triad has already occurred. Our simulation work also shows that it is important to go beyond the static models obtained from molecular docking and include the flexibility of enzyme-ligand complexes in solvents and at a finite temperature. Under such conditions, the pro-drugs studied in this work are unlikely to be hydrolysed by the HCE2 enzyme, indicating a low risk of undesired drug release in normal tissue.
    MeSH term(s) Humans ; Camptothecin/chemistry ; Carboxylesterase/chemistry ; Irinotecan/chemistry ; Molecular Docking Simulation ; Prodrugs/chemistry ; Protein Binding
    Chemical Substances Camptothecin (XT3Z54Z28A) ; Carboxylesterase (EC 3.1.1.1) ; Irinotecan (7673326042) ; Prodrugs ; CES2 protein, human (EC 3.1.1.1)
    Language English
    Publishing date 2024-01-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom14020153
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  9. Article ; Online: In-depth analysis of Gαs protein activity by probing different fluorescently labeled guanine nucleotides.

    Pepanian, Anna / Sommerfeld, Paul / Binbay, Furkan Ayberk / Fischer, Dietmar / Pietsch, Markus / Imhof, Diana

    Biological chemistry

    2024  

    Abstract: G proteins are interacting partners of G protein-coupled receptors (GPCRs) in eukaryotic cells. Upon G protein activation, the ability of the Gα subunit to exchange GDP for GTP determines the intracellular signal transduction. Although various studies ... ...

    Abstract G proteins are interacting partners of G protein-coupled receptors (GPCRs) in eukaryotic cells. Upon G protein activation, the ability of the Gα subunit to exchange GDP for GTP determines the intracellular signal transduction. Although various studies have successfully shown that both Gαs and Gαi have an opposite effect on the intracellular cAMP production, with the latter being commonly described as "more active", the functional analysis of Gαs is a comparably more complicated matter. Additionally, the thorough investigation of the ubiquitously expressed variants of Gαs, Gαs(short) and Gαs(long), is still pending. Since the previous experimental evaluation of the activity and function of the Gαs isoforms is not consistent, the focus was laid on structural investigations to understand the GTPase activity. Herein, we examined recombinant human Gαs by applying an established methodological setup developed for Gαi characterization. The ability for GTP binding was evaluated with fluorescence and fluorescence anisotropy assays, whereas the intrinsic hydrolytic activity of the isoforms was determined by a GTPase assay. Among different nucleotide probes, BODIPY FL GTPγS exhibited the highest binding affinity towards the Gαs subunit. This work provides a deeper understanding of the Gαs subunit and provides novel information concerning the differences between the two protein variants.
    Language English
    Publishing date 2024-02-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1334659-3
    ISSN 1437-4315 ; 1431-6730 ; 1432-0355
    ISSN (online) 1437-4315
    ISSN 1431-6730 ; 1432-0355
    DOI 10.1515/hsz-2023-0321
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  10. Article ; Online: Deferral of non-emergency cardiac interventions is associated with increased emergency hospitalizations up to 24 months post-procedure.

    Andreß, Stefanie / Felbel, Dominik / Buckert, Dominik / Rottbauer, Wolfgang / Imhof, Armin / Stephan, Tilman

    Clinical research in cardiology : official journal of the German Cardiac Society

    2024  

    Abstract: Background: Patients, whose non-emergency cardiac procedure was postponed during the COVID-19 pandemic, have shown signs of disease progression in the short term. Data on the long-term effects are currently lacking.: Aim: To assess outcomes through 3  ...

    Abstract Background: Patients, whose non-emergency cardiac procedure was postponed during the COVID-19 pandemic, have shown signs of disease progression in the short term. Data on the long-term effects are currently lacking.
    Aim: To assess outcomes through 3 years following deferral.
    Methods: This retrospective, single-center analysis includes consecutive patients whose non-emergency cardiovascular intervention was postponed during the first COVID-19-related lockdown (March 19 to April 30, 2020). Outcomes over 36 months post-procedure were analyzed and compared to a seasonal control group undergoing non-emergency intervention in 2019 as scheduled (n = 214). The primary endpoint was a composite of emergency cardiovascular hospitalization and death. Additionally, NT-proBNP levels were analyzed.
    Results: The combined endpoint occurred in 60 of 178 patients (33.7%) whose non-emergency transcatheter heart valve intervention, rhythmological procedure, or left heart catheterization was postponed. Primary endpoint events did not occur more frequently in the study group during the 36-month follow-up (p = 0.402), but within the first 24 months post-procedure (HR 1.77, 95% CI 1.20-2.60, p = 0.003). Deferred patients affected by an event in the postprocedural 24 months had significantly higher NT-proBNP levels at the time of intervention (p < 0.001) (AUC 0.768, p = 0.003, optimum cut-off 808.5 pg/ml, sensitivity 84.2%, specificity 65.8%) and thereafter (p < 0.001).
    Conclusion: Deferral of non-emergency cardiovascular interventions is associated with poor outcomes up to 24 months post-procedure. Adverse effects affect patients who develop signs of acute heart failure, as indicated by NT-proBNP, prior to treatment. These findings could help improve resource allocation in times of limited capacity.
    Language English
    Publishing date 2024-03-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2213295-8
    ISSN 1861-0692 ; 1861-0684
    ISSN (online) 1861-0692
    ISSN 1861-0684
    DOI 10.1007/s00392-024-02380-y
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