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  1. Article ; Online: Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis

    Ren, Deshan / Liu, Wei / Ding, Shuai / Li, Yan

    STAR protocols

    2022  Volume 3, Issue 1, Page(s) 101217

    Abstract: Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis ... ...

    Abstract Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development
    MeSH term(s) Animals ; Flow Cytometry/methods ; Hematopoiesis ; Humans ; Hydrodynamics ; Immune System ; Mice
    Language English
    Publishing date 2022-03-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2022.101217
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  2. Article ; Online: Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo

    Deshan Ren / Wei Liu / Shuai Ding / Yan Li

    STAR Protocols, Vol 3, Iss 1, Pp 101217- (2022)

    2022  

    Abstract: Summary: Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development in vivo. Here, we describe detailed protocols for the construction of HIS mice, modulation of human hematopoiesis in vivo ... ...

    Abstract Summary: Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development in vivo. Here, we describe detailed protocols for the construction of HIS mice, modulation of human hematopoiesis in vivo using hydrodynamic injection of plasmids encoding cytokines of interest, and flow cytometry analysis of humanization levels and human immune subsets. This approach can be easily applied to screen or verify factors that regulate human hematopoiesis and immune system.For complete details on the use and execution of this protocol, please refer to Cardoso et al. (2021) and Li et al. (2017).
    Keywords Cell isolation ; Flow Cytometry/Mass Cytometry ; Immunology ; Model Organisms ; Biotechnology and bioengineering ; Science (General) ; Q1-390
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Lipid Metabolism in Tumor-Associated Myeloid-Derived Suppressor Cells.

    Liu, Wei / Song, Hua / Li, Xiaojing / Ren, Deshan / Ding, Shuai / Li, Yan

    Advances in experimental medicine and biology

    2021  Volume 1316, Page(s) 103–115

    Abstract: Myeloid-derived suppressor cells (MDSCs) are a heterogenous population of myeloid cells with immature phenotypes and immunosuppressive functions. This population of cells has been extensively studied over the past decade owing to an increasing ... ...

    Abstract Myeloid-derived suppressor cells (MDSCs) are a heterogenous population of myeloid cells with immature phenotypes and immunosuppressive functions. This population of cells has been extensively studied over the past decade owing to an increasing recognition of their pivotal role in pathological conditions including cancers, infectious diseases, sepsis, and autoimmune diseases. Various treatments targeting MDSCs are currently under development or in clinical trials with the aim to restore functional immunity against tumors or pathogens. Recent advances in immune metabolism demonstrate the role of metabolic pathways, especially lipid metabolism, in the differentiation and function of MDSCs in tumor environments. Therefore, a comprehensive understanding of lipid metabolism in MDSCs would facilitate the development of novel therapies against tumors through metabolic reprograming of MDSCs.
    MeSH term(s) Autoimmune Diseases ; Humans ; Lipid Metabolism ; Myeloid Cells ; Myeloid-Derived Suppressor Cells ; Neoplasms
    Language English
    Publishing date 2021-03-19
    Publishing country United States
    Document type Journal Article
    ISSN 2214-8019 ; 0065-2598
    ISSN (online) 2214-8019
    ISSN 0065-2598
    DOI 10.1007/978-981-33-6785-2_7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Qindan Capsule Attenuates Myocardial Hypertrophy and Fibrosis in Pressure Overload-Induced Mice Involving mTOR and TGF-

    Bai, Wenwu / Ren, Min / Cheng, Wen / Lu, Xiaoting / Liu, Deshan / Wang, Bo

    Evidence-based complementary and alternative medicine : eCAM

    2021  Volume 2021, Page(s) 5577875

    Abstract: Qindan capsule (QC), a traditional Chinese medicine compound, has been used to treat hypertension in the clinic for over 30 years. It is still not known about the effects of QC on pressure overload-induced cardiac remodeling. Hence, this study aims to ... ...

    Abstract Qindan capsule (QC), a traditional Chinese medicine compound, has been used to treat hypertension in the clinic for over 30 years. It is still not known about the effects of QC on pressure overload-induced cardiac remodeling. Hence, this study aims to investigate the effects of QC on pressure overload-induced cardiac hypertrophy, fibrosis, and heart failure in mice and to determine the possible mechanisms. Transverse aortic constriction (TAC) surgery was used to induce cardiac hypertrophy and heart failure in C57BL/6 mice. Mice were treated with QC or losartan for 8 weeks after TAC surgery. Cardiac function indexes were evaluated with transthoracic echocardiography. Cardiac pathology was detected using HE and Masson's trichrome staining. Cardiomyocyte ultrastructure was detected using transmission electron microscopy. Hypertrophy-related fetal gene expression was investigated using real-time RT-PCR. The expression of 8-OHdG and the concentration of MDA and Ang-II were assessed by immunohistochemistry stain and ELISA assay, respectively. The total and phosphorylated protein levels of mTOR, p70S6K, 4EBP1, Smad2, and Smad3 and the expression of TGF-
    Language English
    Publishing date 2021-04-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2021/5577875
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5995: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  5. Article ; Online: Qindan Capsule Attenuates Myocardial Hypertrophy and Fibrosis in Pressure Overload-Induced Mice Involving mTOR and TGF-β1/Smad Signaling Pathway Inhibition

    Wenwu Bai / Min Ren / Wen Cheng / Xiaoting Lu / Deshan Liu / Bo Wang

    Evidence-Based Complementary and Alternative Medicine, Vol

    2021  Volume 2021

    Abstract: Qindan capsule (QC), a traditional Chinese medicine compound, has been used to treat hypertension in the clinic for over 30 years. It is still not known about the effects of QC on pressure overload-induced cardiac remodeling. Hence, this study aims to ... ...

    Abstract Qindan capsule (QC), a traditional Chinese medicine compound, has been used to treat hypertension in the clinic for over 30 years. It is still not known about the effects of QC on pressure overload-induced cardiac remodeling. Hence, this study aims to investigate the effects of QC on pressure overload-induced cardiac hypertrophy, fibrosis, and heart failure in mice and to determine the possible mechanisms. Transverse aortic constriction (TAC) surgery was used to induce cardiac hypertrophy and heart failure in C57BL/6 mice. Mice were treated with QC or losartan for 8 weeks after TAC surgery. Cardiac function indexes were evaluated with transthoracic echocardiography. Cardiac pathology was detected using HE and Masson’s trichrome staining. Cardiomyocyte ultrastructure was detected using transmission electron microscopy. Hypertrophy-related fetal gene expression was investigated using real-time RT-PCR. The expression of 8-OHdG and the concentration of MDA and Ang-II were assessed by immunohistochemistry stain and ELISA assay, respectively. The total and phosphorylated protein levels of mTOR, p70S6K, 4EBP1, Smad2, and Smad3 and the expression of TGF-β1 and collagen I were measured using western blot. The results showed that low- and high-dose QC improved pressure overload-induced cardiac hypertrophy, fibrosis, and dysfunction. QC inhibited ANP, BNP, and β-MHC mRNA expression in failing hearts. QC improved myocardial ultrastructure after TAC surgery. Furthermore, QC downregulated the expression of 8-OHdG and the concentration of MDA, 15-F2t-IsoP, and Ang-II in heart tissues after TAC surgery. We also found that QC inhibited the phosphorylation of mTOR, p70S6K, and 4EBP1 and the expression of TGF-β1, p-Smad2, p-Smad3, and collagen I in pressure overload-induced failing hearts. These data indicate that QC has direct benefic effects on pressure overload-induced cardiac hypertrophy, fibrosis, and dysfunction. The protective effects of QC involve prevention of increased oxidative stress injury and Ang-II levels and ...
    Keywords Other systems of medicine ; RZ201-999
    Subject code 616
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Immediate and long-term changes in the epidemiology, infection spectrum, and clinical characteristics of viral and bacterial respiratory infections in Western China after the COVID-19 outbreak: a modeling study

    Shi, Tianshan / Zhao, Xin / Zhang, Xiaoshu / Meng, Lei / Li, Donghua / Liu, Xinfeng / Zheng, Hongmiao / Yu, Deshan / Wang, Tingrong / Li, Rui / Li, Juansheng / Shen, Xiping / Ren, Xiaowei

    Arch Virol. 2023 Apr., v. 168, no. 4 p.120-120

    2023  

    Abstract: BACKGROUND: The impact of COVID-19 on the epidemiology, clinical characteristics, and infection spectrum of viral and bacterial respiratory infections in Western China is unknown. METHODS: We conducted an interrupted time series analysis based on ... ...

    Abstract BACKGROUND: The impact of COVID-19 on the epidemiology, clinical characteristics, and infection spectrum of viral and bacterial respiratory infections in Western China is unknown. METHODS: We conducted an interrupted time series analysis based on surveillance of acute respiratory infections (ARI) in Western China to supplement the available data. RESULTS: The positive rates of influenza virus, Streptococcus pneumoniae, and viral and bacterial coinfections decreased, but parainfluenza virus, respiratory syncytial virus, human adenovirus, human rhinovirus, human bocavirus, non-typeable Haemophilus influenzae, Mycoplasma pneumoniae, and Chlamydia pneumoniae infections increased after the onset of the COVID-19 epidemic. The positive rate for viral infection in outpatients and children aged <5 years increased, but the positive rates of bacterial infection and viral and bacterial coinfections decreased, and the proportion patients with clinical symptoms of ARI decreased after the onset of the COVID-19 epidemic. Non-pharmacological interventions reduced the positive rates of viral and bacterial infections in the short term but did not have a long-term limiting effect. Moreover, the proportion of ARI patients with severe clinical symptoms (dyspnea and pleural effusion) increased in the short term after COVID-19, but in the long-term, it decreased. CONCLUSIONS: The epidemiology, clinical characteristics, and infection spectrum of viral and bacterial infections in Western China have changed, and children will be a high-risk group for ARI after the COVID-19 epidemic. In addition, the reluctance of ARI patients with mild clinical symptoms to seek medical care after COVID-19 should be considered. In the post-COVID-19 era, we need to strengthen the surveillance of respiratory pathogens.
    Keywords Bocaparvovirus ; COVID-19 infection ; Chlamydophila pneumoniae ; Haemophilus influenzae ; Human rhinovirus ; Mastadenovirus ; Mycoplasma pneumoniae ; Orthomyxoviridae ; Respiratory syncytial virus ; Streptococcus pneumoniae ; bacterial infections ; dyspnea ; epidemiology ; monitoring ; time series analysis ; viruses ; China
    Language English
    Dates of publication 2023-04
    Size p. 120.
    Publishing place Springer Vienna
    Document type Article ; Online
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-023-05752-3
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  7. Article ; Online: Oncolytic Newcastle disease virus expressing the co-stimulator OX40L as immunopotentiator for colorectal cancer therapy.

    Tian, Limin / Liu, Tianyan / Jiang, Shan / Cao, Yukai / Kang, Kai / Su, Han / Ren, Guiping / Wang, Zhenzhong / Xiao, Wei / Li, Deshan

    Gene therapy

    2021  Volume 30, Issue 1-2, Page(s) 64–74

    Abstract: NDV as an attractive candidate for oncolytic immunotherapy selectively lyses tumor cells but shows limited anti-tumor immunity. Immune co-stimulator OX40 ligand (OX40L) boosts anti-tumor immunity response by delivering a potent costimulatory signal to ... ...

    Abstract NDV as an attractive candidate for oncolytic immunotherapy selectively lyses tumor cells but shows limited anti-tumor immunity. Immune co-stimulator OX40 ligand (OX40L) boosts anti-tumor immunity response by delivering a potent costimulatory signal to CD4
    MeSH term(s) Animals ; Mice ; Newcastle disease virus/genetics ; Newcastle disease virus/metabolism ; CD8-Positive T-Lymphocytes ; Adjuvants, Immunologic/metabolism ; OX40 Ligand/genetics ; OX40 Ligand/metabolism ; Oncolytic Viruses/genetics ; Interleukin-2 ; Colorectal Neoplasms/therapy
    Chemical Substances Adjuvants, Immunologic ; OX40 Ligand ; Interleukin-2
    Language English
    Publishing date 2021-10-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1191036-7
    ISSN 1476-5462 ; 0969-7128
    ISSN (online) 1476-5462
    ISSN 0969-7128
    DOI 10.1038/s41434-021-00256-8
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3991: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Immediate and long-term changes in the epidemiology, infection spectrum, and clinical characteristics of viral and bacterial respiratory infections in Western China after the COVID-19 outbreak: a modeling study.

    Shi, Tianshan / Zhao, Xin / Zhang, Xiaoshu / Meng, Lei / Li, Donghua / Liu, Xinfeng / Zheng, Hongmiao / Yu, Deshan / Wang, Tingrong / Li, Rui / Li, Juansheng / Shen, Xiping / Ren, Xiaowei

    Archives of virology

    2023  Volume 168, Issue 4, Page(s) 120

    Abstract: Background: The impact of COVID-19 on the epidemiology, clinical characteristics, and infection spectrum of viral and bacterial respiratory infections in Western China is unknown.: Methods: We conducted an interrupted time series analysis based on ... ...

    Abstract Background: The impact of COVID-19 on the epidemiology, clinical characteristics, and infection spectrum of viral and bacterial respiratory infections in Western China is unknown.
    Methods: We conducted an interrupted time series analysis based on surveillance of acute respiratory infections (ARI) in Western China to supplement the available data.
    Results: The positive rates of influenza virus, Streptococcus pneumoniae, and viral and bacterial coinfections decreased, but parainfluenza virus, respiratory syncytial virus, human adenovirus, human rhinovirus, human bocavirus, non-typeable Haemophilus influenzae, Mycoplasma pneumoniae, and Chlamydia pneumoniae infections increased after the onset of the COVID-19 epidemic. The positive rate for viral infection in outpatients and children aged <5 years increased, but the positive rates of bacterial infection and viral and bacterial coinfections decreased, and the proportion patients with clinical symptoms of ARI decreased after the onset of the COVID-19 epidemic. Non-pharmacological interventions reduced the positive rates of viral and bacterial infections in the short term but did not have a long-term limiting effect. Moreover, the proportion of ARI patients with severe clinical symptoms (dyspnea and pleural effusion) increased in the short term after COVID-19, but in the long-term, it decreased.
    Conclusions: The epidemiology, clinical characteristics, and infection spectrum of viral and bacterial infections in Western China have changed, and children will be a high-risk group for ARI after the COVID-19 epidemic. In addition, the reluctance of ARI patients with mild clinical symptoms to seek medical care after COVID-19 should be considered. In the post-COVID-19 era, we need to strengthen the surveillance of respiratory pathogens.
    MeSH term(s) Child ; Humans ; Infant ; COVID-19/epidemiology ; Coinfection/epidemiology ; Respiratory Tract Infections/epidemiology ; Bacterial Infections/epidemiology ; Bacterial Infections/diagnosis ; China/epidemiology ; Bacteria ; Disease Outbreaks
    Language English
    Publishing date 2023-03-28
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-023-05752-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.110: Show issues Location:
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  9. Article ; Online: TLR7, a third signal for the robust generation of spontaneous germinal center B cells in systemic lupus erythematosus.

    Fan, Hongye / Ren, Deshan / Hou, Yayi

    Cellular & molecular immunology

    2017  Volume 15, Issue 3, Page(s) 286–288

    MeSH term(s) Animals ; Antibodies, Antinuclear/blood ; B-Lymphocytes/immunology ; Cell Differentiation ; Clonal Selection, Antigen-Mediated ; Cyclin D3/genetics ; Disease Models, Animal ; Germinal Center/immunology ; Humans ; Immune Tolerance ; Lupus Erythematosus, Systemic/immunology ; Lymphocyte Activation ; Mice ; Mice, Knockout ; Receptors, Antigen, B-Cell/metabolism ; Signal Transduction ; T-Lymphocytes, Helper-Inducer/immunology ; Toll-Like Receptor 7/genetics ; Toll-Like Receptor 7/metabolism
    Chemical Substances Antibodies, Antinuclear ; Ccnd3 protein, mouse ; Cyclin D3 ; Receptors, Antigen, B-Cell ; Toll-Like Receptor 7
    Language English
    Publishing date 2017-11-27
    Publishing country China
    Document type Letter
    ZDB-ID 2435097-7
    ISSN 2042-0226 ; 1672-7681
    ISSN (online) 2042-0226
    ISSN 1672-7681
    DOI 10.1038/cmi.2017.123
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6681: Show issues Location:
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  10. Article ; Online: Therapeutic effect and mechanism of combined use of FGF21 and insulin on diabetic nephropathy.

    Meng, Fanrui / Cao, Yukai / Khoso, Mir Hassan / Kang, Kai / Ren, Guiping / Xiao, Wei / Li, Deshan

    Archives of biochemistry and biophysics

    2021  Volume 713, Page(s) 109063

    Abstract: Although FGF21 ameliorates diabetic nephropathy (DN), the efficacy is not satisfactory. Studies demonstrate that FGF21 combined with Insulin exhibits reciprocal sensitization on glucose and lipid metabolism in mice with type 2 diabetes. However, ... ...

    Abstract Although FGF21 ameliorates diabetic nephropathy (DN), the efficacy is not satisfactory. Studies demonstrate that FGF21 combined with Insulin exhibits reciprocal sensitization on glucose and lipid metabolism in mice with type 2 diabetes. However, therapeutic effect of combined use of FGF21 and Insulin on DN has not been reported. Therefore, this study explored therapeutic effect and mechanism of combined use of FGF21 and Insulin on DN. Our results showed that compared with Insulin or FGF21 alone, FGF21 combined with Insulin further ameliorated blood glucose, HbAlc, OGTT, renal function, liver function, blood lipid, histopathological changes, oxidative stress and AGEs in the mice of DN (BKS-Lepr
    MeSH term(s) Animals ; Autophagy/drug effects ; Blood Glucose/metabolism ; Diabetic Nephropathies/drug therapy ; Diabetic Nephropathies/pathology ; Drug Combinations ; Fibroblast Growth Factors/therapeutic use ; Glycation End Products, Advanced/metabolism ; Hypoglycemic Agents/therapeutic use ; Inflammation/drug therapy ; Inflammation/pathology ; Insulin/therapeutic use ; Kidney/drug effects ; Kidney/pathology ; Male ; Mice ; Oxidative Stress/drug effects ; Signal Transduction/drug effects
    Chemical Substances Blood Glucose ; Drug Combinations ; Glycation End Products, Advanced ; Hypoglycemic Agents ; Insulin ; fibroblast growth factor 21 ; Fibroblast Growth Factors (62031-54-3)
    Language English
    Publishing date 2021-10-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 523-x
    ISSN 1096-0384 ; 0003-9861
    ISSN (online) 1096-0384
    ISSN 0003-9861
    DOI 10.1016/j.abb.2021.109063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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