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Article ; Online: Acute Flaccid Paralysis Associated with Novel Enterovirus C105.

Horner, Liana M / Poulter, Melinda D / Brenton, J Nicholas / Turner, Ronald B

2015 Volume 21, Issue 10, Page(s) 1858–1860

Abstract: An outbreak of acute flaccid paralysis among children in the United States during summer 2014 was tentatively associated with enterovirus D68 infection. This syndrome in a child in fall 2014 was associated with enterovirus C105 infection. The presence of ...

Abstract	An outbreak of acute flaccid paralysis among children in the United States during summer 2014 was tentatively associated with enterovirus D68 infection. This syndrome in a child in fall 2014 was associated with enterovirus C105 infection. The presence of this virus strain in North America may pose a diagnostic challenge.
MeSH term(s)	Child ; Disease Outbreaks ; Enterovirus C, Human/classification ; Enterovirus C, Human/pathogenicity ; Enterovirus D, Human/pathogenicity ; Enterovirus Infections/diagnosis ; Enterovirus Infections/pathology ; Female ; Humans ; Muscle Hypotonia/virology ; Paralysis/virology ; Virginia/epidemiology
Language	English
Publishing date	2015-09-17
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2110.150759
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Article ; Online: Acute Flaccid Paralysis Associated with Novel Enterovirus C105

Liana M. Horner / Melinda D. Poulter / J. Nicholas Brenton / Ronald B. Turner

Emerging Infectious Diseases, Vol 21, Iss 10, Pp 1858-

2015 Volume 1860

Abstract	An outbreak of acute flaccid paralysis among children in the United States during summer 2014 was tentatively associated with enterovirus D68 infection. This syndrome in a child in fall 2014 was associated with enterovirus C105 infection. The presence of this virus strain in North America may pose a diagnostic challenge.
Keywords	enterovirus ; rhinovirus ; flaccid paralysis ; viruses ; enterovirus C105 ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
Language	English
Publishing date	2015-10-01T00:00:00Z
Publisher	Centers for Disease Control and Prevention
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online ; Research data: (with research data) Extensive clonal spread and extreme longevity in saw palmetto, a foundation clonal plant.

Takahashi, Mizuki K / Horner, Liana M / Kubota, Toshiro / Keller, Nathan A / Abrahamson, Warren G

Molecular ecology

2011 Volume 20, Issue 18, Page(s) 3730–3742

Abstract: ... a sympatric non-clonal palmetto) samples collected from a 20 × 20 m study plot in Florida scrub. Sabal samples ...

Abstract	The lack of effective tools has hampered out ability to assess the size, growth and ages of clonal plants. With Serenoa repens (saw palmetto) as a model, we introduce a novel analytical framework that integrates DNA fingerprinting and mathematical modelling to simulate growth and estimate ages of clonal plants. We also demonstrate the application of such life-history information of clonal plants to provide insight into management plans. Serenoa is an ecologically important foundation species in many Southeastern United States ecosystems; yet, many land managers consider Serenoa a troublesome invasive plant. Accordingly, management plans have been developed to reduce or eliminate Serenoa with little understanding of its life history. Using Amplified Fragment Length Polymorphisms, we genotyped 263 Serenoa and 134 Sabal etonia (a sympatric non-clonal palmetto) samples collected from a 20 × 20 m study plot in Florida scrub. Sabal samples were used to assign small field-unidentifiable palmettos to Serenoa or Sabal and also as a negative control for clone detection. We then mathematically modelled clonal networks to estimate genet ages. Our results suggest that Serenoa predominantly propagate via vegetative sprouts and 10,000-year-old genets may be common, while showing no evidence of clone formation by Sabal. The results of this and our previous studies suggest that: (i) Serenoa has been part of scrub associations for thousands of years, (ii) Serenoa invasion are unlikely and (ii) once Serenoa is eliminated from local communities, its restoration will be difficult. Reevaluation of the current management tools and plans is an urgent task.
MeSH term(s)	Amplified Fragment Length Polymorphism Analysis ; Conservation of Natural Resources/methods ; DNA Fingerprinting/methods ; Florida ; Longevity/physiology ; Models, Genetic ; Reproduction, Asexual/physiology ; Serenoa/genetics ; Serenoa/physiology ; Species Specificity
Language	English
Publishing date	2011-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1126687-9
ISSN	1365-294X ; 0962-1083 ; 0962-1083
ISSN (online)	1365-294X
ISSN	0962-1083
DOI	10.1111/j.1365-294X.2011.05212.x
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Article ; Research data: (with research data) Extensive clonal spread and extreme longevity in saw palmetto, a foundation clonal plant

TAKAHASHI, MIZUKI K / HORNER, LIANA M / KUBOTA, TOSHIRO / KELLER, NATHAN A / ABRAHAMSON, WARREN G

Molecular ecology. 2011 Sept., v. 20, no. 18

2011

Abstract: ... a sympatric non‐clonal palmetto) samples collected from a 20 × 20 m study plot in Florida scrub. Sabal samples ...

Abstract	The lack of effective tools has hampered out ability to assess the size, growth and ages of clonal plants. With Serenoa repens (saw palmetto) as a model, we introduce a novel analytical framework that integrates DNA fingerprinting and mathematical modelling to simulate growth and estimate ages of clonal plants. We also demonstrate the application of such life‐history information of clonal plants to provide insight into management plans. Serenoa is an ecologically important foundation species in many Southeastern United States ecosystems; yet, many land managers consider Serenoa a troublesome invasive plant. Accordingly, management plans have been developed to reduce or eliminate Serenoa with little understanding of its life history. Using Amplified Fragment Length Polymorphisms, we genotyped 263 Serenoa and 134 Sabal etonia (a sympatric non‐clonal palmetto) samples collected from a 20 × 20 m study plot in Florida scrub. Sabal samples were used to assign small field‐unidentifiable palmettos to Serenoa or Sabal and also as a negative control for clone detection. We then mathematically modelled clonal networks to estimate genet ages. Our results suggest that Serenoa predominantly propagate via vegetative sprouts and 10 000‐year‐old genets may be common, while showing no evidence of clone formation by Sabal. The results of this and our previous studies suggest that: (i) Serenoa has been part of scrub associations for thousands of years, (ii) Serenoa invasion are unlikely and (ii) once Serenoa is eliminated from local communities, its restoration will be difficult. Reevaluation of the current management tools and plans is an urgent task.
Keywords	DNA fingerprinting ; Sabal palmetto ; Serenoa repens ; amplified fragment length polymorphism ; clones ; ecosystems ; growth models ; invasive species ; life history ; longevity ; managers ; mathematical models ; plant age ; shrublands ; Florida
Language	English
Dates of publication	2011-09
Size	p. 3730-3742.
Publishing place	Blackwell Publishing Ltd
Document type	Article ; Research data
ZDB-ID	1126687-9
ISSN	1365-294X ; 0962-1083 ; 0962-1083
ISSN (online)	1365-294X
ISSN	0962-1083
DOI	10.1111/j.1365-294X.2011.05212.x
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Article: Yoga induced acute ulnar nerve compression by a ganglion cyst in Guyon's canal.

Horner, Liana / Edelsohn, Lanny / Gakhal, Mandip

Delaware medical journal

2013 Volume 85, Issue 12, Page(s) 369–373

Abstract: Acute ulnar neuropathy at the wrist can be difficult to diagnose, as it is an uncommon neuropathy with variable clinical presentations and numerous etiologies. We present a case of acute ulnar neuropathy of the deep motor branch caused by a ganglion cyst ...

Abstract	Acute ulnar neuropathy at the wrist can be difficult to diagnose, as it is an uncommon neuropathy with variable clinical presentations and numerous etiologies. We present a case of acute ulnar neuropathy of the deep motor branch caused by a ganglion cyst in Guyon's canal. Interestingly, this case of acute loss of motor function occurred after the patient participated in yoga (specifically the downward dog position), and resolved spontaneously over time after stopping yoga, without surgical excision of the ganglion, suggesting exacerbation or protrusion of an occult ganglion cyst due to increased activity and compression of the hypothenar eminence.
MeSH term(s)	Female ; Ganglion Cysts/complications ; Humans ; Magnetic Resonance Imaging ; Middle Aged ; Ulnar Nerve Compression Syndromes/etiology ; Wrist/pathology ; Yoga
Language	English
Publishing date	2013-12
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	428380-6
ISSN	0011-7781
ISSN	0011-7781
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Article ; Online: Surfactant administration prior to one lung ventilation: physiological and inflammatory correlates in a piglet model.

Bhatia, Rahul / Shaffer, Thomas H / Hossain, Jobayer / Fisher, Alicia Olivant / Horner, Liana M / Rodriguez, M Elena / Penfil, Scott / Theroux, Mary C

Pediatric pulmonology

2011 Volume 46, Issue 11, Page(s) 1069–1078

Abstract: Objectives: To test the hypothesis that surfactant, when given prophylactically during one lung ventilation (OLV), improves physiological stability and reduces inflammation.: Methods: Prospective controlled animal study. After 30 min of mechanical ... ...

Abstract	Objectives: To test the hypothesis that surfactant, when given prophylactically during one lung ventilation (OLV), improves physiological stability and reduces inflammation. Methods: Prospective controlled animal study. After 30 min of mechanical ventilation, surfactant was administered to the left lung of the treatment group. Right lung mechanical ventilation continued for 3 hr, after which the left lung was unblocked. Bilateral mechanical ventilation was continued for 30 min thereafter. Physiological parameters and biomarkers of inflammation in plasma, lung tissue homogenates, and bronchoalveolar lavage (BAL) were measured. Measurements and main results: Oxygenation improved in the surfactant group, reaching statistical significance at 3 hr of OLV and again after 30 min of bilateral mechanical ventilation following the OLV. Plasma levels of interleukin (IL)-1 β, IL-6, and tumor necrosis factor (TNF)-α showed a trend for reduction. The lung homogenates from the ventilated lungs had significantly lower levels of IL-1 β (P < 0.01) and IL-6 (P < 0.01). The BAL specimen showed an overall reduction in the cytokine levels; IL-1 β was significantly lower in the ventilated lungs (P < 0.01). Conclusions: Surfactant administration improves oxygenation and decreases inflammation, as evidenced by a decrease in several inflammatory cytokines both in the plasma and lungs of a piglet model of OLV.
MeSH term(s)	Acute Lung Injury/etiology ; Acute Lung Injury/prevention & control ; Animals ; Biological Products/administration & dosage ; Biomarkers/analysis ; Bronchoalveolar Lavage Fluid/chemistry ; Disease Models, Animal ; Female ; Interleukin-1beta/blood ; Interleukin-6/blood ; Lung/chemistry ; Lung/drug effects ; Male ; Oxygen Consumption/drug effects ; Pneumonia/etiology ; Pneumonia/prevention & control ; Pulmonary Surfactants/administration & dosage ; Respiration, Artificial/adverse effects ; Swine ; Tumor Necrosis Factor-alpha/blood
Chemical Substances	Biological Products ; Biomarkers ; Interleukin-1beta ; Interleukin-6 ; Pulmonary Surfactants ; Tumor Necrosis Factor-alpha ; calfactant (Q4K217VGA9)
Language	English
Publishing date	2011-05-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	632784-9
ISSN	1099-0496 ; 8755-6863
ISSN (online)	1099-0496
ISSN	8755-6863
DOI	10.1002/ppul.21485
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Article ; Online: Protective ventilation to reduce inflammatory injury from one lung ventilation in a piglet model.

Theroux, Mary C / Fisher, Alicia O / Horner, Liana M / Rodriguez, Maria E / Costarino, Andrew T / Miller, Thomas L / Shaffer, Thomas H

Paediatric anaesthesia

2009 Volume 20, Issue 4, Page(s) 356–364

Abstract: Objectives: To test the hypothesis that protective ventilation strategy (PVS) as defined by the use of low stretch ventilation (tidal volume of 5 ml x kg(-1) and employing 5 cm of positive end expiratory pressure (PEEP) during one lung ventilation (OLV) ...

Abstract	Objectives: To test the hypothesis that protective ventilation strategy (PVS) as defined by the use of low stretch ventilation (tidal volume of 5 ml x kg(-1) and employing 5 cm of positive end expiratory pressure (PEEP) during one lung ventilation (OLV) in piglets would result in reduced injury compared to a control group of piglets who received the conventional ventilation (tidal volume of 10 ml x kg(-1) and no PEEP). Background: PVS has been found to be beneficial in adults to minimize injury from OLV. We designed the current study to test the beneficial effects of PVS in a piglet model of OLV. Methods: Ten piglets each were assigned to either 'Control' group (tidal volume of 10 ml x kg(-1) and no PEEP) or 'PVS' group (tidal volume of 5 ml x kg(-1) during the OLV phase and PEEP of 5 cm of H2O throughout the study). Experiment consisted of 30 min of baseline ventilation, 3 h of OLV, and again 30 min of bilateral ventilation. Respiratory parameters and proinflammatory markers were measured as outcome. Results: There was no difference in PaO2 between groups. PaCO2 (P < 0.01) and ventilatory rate (P < 0.01) were higher at 1.5 h OLV and at the end point in the PVS group. Peak inflating pressure (PIP) and pulmonary resistance were higher (P < 0.05) in the control group at 1.5 h OLV. tumor necrosis factor-alpha (P < 0.04) and IL-8 were less (P < 0.001) in the plasma from the PVS group, while IL-6 and IL-8 were less (P < 0.04) in the lung tissue from ventilated lungs in the PVS group. Conclusions: Based on this model, PVS decreases inflammatory injury both systemically and in the lung tissue with no adverse effect on oxygenation, ventilation, or lung function.
MeSH term(s)	Animals ; Animals, Newborn ; Cytokines/metabolism ; Disease Models, Animal ; Lung/metabolism ; Pneumonia/complications ; Pneumonia/prevention & control ; Positive-Pressure Respiration/methods ; Respiration, Artificial/adverse effects ; Swine ; Tidal Volume ; Tumor Necrosis Factor-alpha/metabolism ; Vascular Resistance ; Ventilator-Induced Lung Injury/etiology ; Ventilator-Induced Lung Injury/prevention & control
Chemical Substances	Cytokines ; Tumor Necrosis Factor-alpha
Language	English
Publishing date	2009-11-16
Publishing country	France
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1086049-6
ISSN	1460-9592 ; 1155-5645
ISSN (online)	1460-9592
ISSN	1155-5645
DOI	10.1111/j.1460-9592.2009.03195.x
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Article ; Online: Defining dose-response relationships in the therapeutic blockade of B7RP-1-dependent immune responses.

Metz, Daniela P / Mohn, Deanna / Zhang, Ming / Horan, Tom / Kim, Helen / Deshpande, Rohini / Jacobsen, Frederick / Shen, Wenyan D / Zhang, Liana / Doellgast, George / Narayanan, Adimoolam / Dalphin, Mark / Kuchimanchi, Kameswara Rao V / Horner, Michelle / Chung, James / Siu, Gerald

European journal of pharmacology

2009 Volume 610, Issue 1-3, Page(s) 110–118

Abstract: The ICOS (Inducible T cell Co-Stimulator)/B7RP-1 (B7-related protein 1) interaction is critical for the proper activation of a T lymphocyte. In this manuscript we describe a systematic in vivo approach to determine the level of blockade required to ... ...

Abstract	The ICOS (Inducible T cell Co-Stimulator)/B7RP-1 (B7-related protein 1) interaction is critical for the proper activation of a T lymphocyte. In this manuscript we describe a systematic in vivo approach to determine the level of blockade required to impair the generation of a T cell-dependent antibody response. We have developed an overall strategy for correlating drug exposure, target saturation, and efficacy in a biological response that can be generalized for most protein therapeutics. Using this strategy, we determined that low levels of B7RP-1 blockade are still sufficient to inhibit the immune response. These data suggest that contact between the T cell and the antigen-presenting cell during antigen presentation is much more sensitive to inhibition than previously believed and that ICOS/B7RP-1 blockade may be efficacious in the treatment of autoimmune diseases.
MeSH term(s)	Aluminum Hydroxide/immunology ; Animals ; Antibodies, Monoclonal/pharmacology ; Antigen-Presenting Cells/immunology ; Antigens, CD19/metabolism ; B-Lymphocytes/metabolism ; B7-1 Antigen/genetics ; B7-1 Antigen/pharmacology ; Binding Sites ; CD3 Complex/metabolism ; Cytokines/blood ; Dose-Response Relationship, Drug ; Female ; Fluorescein-5-isothiocyanate/metabolism ; Fluorescent Dyes/metabolism ; Hemocyanins/immunology ; Immune System Phenomena/drug effects ; Inducible T-Cell Co-Stimulator Ligand ; Mice ; Mice, Inbred BALB C ; Models, Immunological ; Protein Binding ; Recombinant Fusion Proteins/pharmacology ; T-Lymphocytes/metabolism ; Temperature ; Time Factors
Chemical Substances	Antibodies, Monoclonal ; Antigens, CD19 ; B7-1 Antigen ; CD3 Complex ; Cytokines ; Fluorescent Dyes ; Inducible T-Cell Co-Stimulator Ligand ; Recombinant Fusion Proteins ; Aluminum Hydroxide (5QB0T2IUN0) ; Hemocyanins (9013-72-3) ; keyhole-limpet hemocyanin (FV4Y0JO2CX) ; Fluorescein-5-isothiocyanate (I223NX31W9)
Language	English
Publishing date	2009-05-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80121-5
ISSN	1879-0712 ; 0014-2999
ISSN (online)	1879-0712
ISSN	0014-2999
DOI	10.1016/j.ejphar.2009.03.023
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