LIVIVO - Search results -

Search results

Result 1 - 10 of total 4822

Search options

Article ; Online: The O-glycosylating enzyme GALNT2 acts as an oncogenic driver in non-small cell lung cancer.

Hu, Qing / Tian, Tian / Leng, Yahui / Tang, Yuanhui / Chen, Shuang / Lv, Yueyao / Liang, Jingyin / Liu, Yanni / Liu, Tianhui / Shen, Li / Dong, Xiaoxia

Cellular & molecular biology letters

2022 Volume 27, Issue 1, Page(s) 71

Abstract: Background: N-Acetylgalactosaminyltransferases (GALNTs), the enzymes that initiate mucin-type O ... revealed that GALNT2 modified the O-glycosylation of ITGA5 and affected the activation of the PI3K/Akt and ...

Abstract	Background: N-Acetylgalactosaminyltransferases (GALNTs), the enzymes that initiate mucin-type O-glycosylation, are closely associated with tumor occurrence and progression. However, a comprehensive analysis of GALNTs in non-small cell lung cancer (NSCLC) is lacking. Methods: The expression profiles and prognostic values of the GALNT family members in NSCLC were analyzed using publicly available databases. Gain- and loss-of-function experiments were applied to assess the biological function of GALNT2 in NSCLC. High-throughput sequencing and bioinformatics approaches were employed to uncover the regulatory mechanism of GALNT2. Results: Among the family members of GALNTs, only GALNT2 was frequently overexpressed in NSCLC tissues and was positively correlated with poor prognosis. In vitro assays showed that GALNT2 knockdown repressed NSCLC cell proliferation, migration, and invasion, but induced apoptosis and cell cycle arrest. Correspondently, GALNT2 overexpression exerted the opposite effects. In vivo experiments demonstrated that knockdown of GALNT2 restrained tumor formation in nude mice. Mechanistic investigations revealed that GALNT2 modified the O-glycosylation of ITGA5 and affected the activation of the PI3K/Akt and MAPK/ERK pathways. Further studies showed that miR-30d was a negative regulator of GALNT2. Conclusions: These findings suggest that GALNT2 is an oncogene in NSCLC and has the potential as a target for NSCLC therapy.
MeSH term(s)	Animals ; Carcinogenesis/genetics ; Carcinoma, Non-Small-Cell Lung/pathology ; Lung Neoplasms/pathology ; Mice ; Mice, Nude ; N-Acetylgalactosaminyltransferases/metabolism ; Oncogenes ; Phosphatidylinositol 3-Kinases/metabolism ; Polypeptide N-acetylgalactosaminyltransferase
Chemical Substances	N-Acetylgalactosaminyltransferases (EC 2.4.1.-)
Language	English
Publishing date	2022-09-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2108724-6
ISSN	1689-1392 ; 1689-1392
ISSN (online)	1689-1392
ISSN	1689-1392
DOI	10.1186/s11658-022-00378-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- Full text online
- Order with fees

Article ; Online: Manganese losses induced by severe soil acidification in the extensive Lei bamboo (Phyllostachys violascens) plantation stands in Eastern China.

Zong, Yutong / Li, Zichuan / Gui, Renyi / Chen, De / Yuan, Mengting / Chai, Yanjun / Shan, Shengdao / Wong, Ming Hung

Chemosphere

2023 Volume 339, Page(s) 139669

Abstract: ... managed Lei bamboo (Phyllostachys violascens) stands are usually subjected to severe soil acidification ... in a chronosequence of Lei bamboo stands (0, 2, 6, 11, and 16 years of stand age). The results showed that the Mn ...

Abstract	Manganese (Mn) is a critical element in soils, essential to plant growth. Long-term and intensively managed Lei bamboo (Phyllostachys violascens) stands are usually subjected to severe soil acidification and Mn activation. However, Mn migration from topsoil to deep soil induced by severe soil acidification was poorly recognized and studied. The distribution and changes of the total and the operationally defined Mn forms in soil profiles and its potential stress and environmental effect were investigated in a chronosequence of Lei bamboo stands (0, 2, 6, 11, and 16 years of stand age). The results showed that the Mn amount was significantly decreased in topsoil and accumulated in subsoil with the long-term and intensive fertilizer application. Soil exchangeable Mn and superphosphate extractable Mn demonstrated large different variation to total Mn, whereas their sum was largely higher than and highly correlated with 8-hydroxyquinoline (HQN) extractable Mn. Soil organic carbon, pH value, exchangeable bases, and soil redox simultaneously controlled soil Mn depletion. In conclusion, long-term and intensive fertilizer application leads to soil acidification and accelerated soil Mn depletion in bamboo stand soil, promoting Mn accumulation in bamboo shoots.
MeSH term(s)	Soil ; Carbon/analysis ; Manganese ; Fertilizers ; Poaceae ; China ; Hydrogen-Ion Concentration
Chemical Substances	Soil ; Carbon (7440-44-0) ; Manganese (42Z2K6ZL8P) ; Fertilizers
Language	English
Publishing date	2023-07-30
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2023.139669
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2540: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Winter mulching practice alters soil bacterial communities and networks in lei bamboo (Phyllostachys praecox) forests

Wu, Fei / Wu, Na / Zhang, Linping / Li, Zuyao / Pei, Nancai / Jin, Cangfu / Huang, Yuxuan

Land Degradation & Development. 2023 May 30, v. 34, no. 9 p.2535-2547

2023

Abstract: ... wheat straw, WS) on soil bacterial communities and networks in lei bamboo (Phyllostachys praecox) forests over ...

Abstract	Winter mulching is widely used to raise soil temperature and promote earlier harvesting in agroforestry, but successive years of mulching can lead to soil degradation. The effect of winter mulch on soil bacterial community composition and co‐occurrence patterns is not clear. The influence of different mulches (a defatted seed cake of Camellia oleifera, DS; a mixture of wheat straw and chicken manure, MWC; and wheat straw, WS) on soil bacterial communities and networks in lei bamboo (Phyllostachys praecox) forests over a four‐month mulching period was studied using Illumina sequencing of the 16S rRNA gene. The results showed that mulching time and mulching material treatment significantly influenced bacterial community composition and diversity, mainly driven by nutrient availability. MWC and WS mulching treatments decreased α‐diversity of the bacterial communities, whereas the DS treatment had no significant impact. The oligotrophs, Gryllotalpicola, Granulicella, and Verrucomicrobia, were enriched before and at the end of mulching, while the copiotrophs, Deltaproteobacteria, were enriched in the middle of the mulching period (Jan). Comparing the three mulching treatments, Proteobacteria were enriched in DS mulching soils in Jan. The complexity of the four networks decreased with pre‐mulching, DS mulching, WS mulching, and MWC mulching networks, with a reduction in the number of edges and average degree observed. The results suggested that winter mulching practices adversely affected soil bacterial community diversity and interactions, which may be detrimental to the resistance of bacterial communities to environmental changes and soil sustainability. Mulching MWC and WS has a greater risk of loss of soil bacterial biodiversity than DS.
Keywords	Camellia oleifera ; Phyllostachys violascens ; Verrucomicrobia ; agroforestry ; bacterial communities ; bamboos ; biodiversity ; community structure ; delta-Proteobacteria ; genes ; land degradation ; mulches ; nutrient availability ; oilseed cakes ; poultry manure ; risk ; soil bacteria ; soil degradation ; soil temperature ; wheat straw
Language	English
Dates of publication	2023-0530
Size	p. 2535-2547.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	1319202-4
ISSN	1085-3278
ISSN	1085-3278
DOI	10.1002/ldr.4626
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 5058: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Manganese losses induced by severe soil acidification in the extensive Lei bamboo (Phyllostachys violascens) plantation stands in Eastern China

Zong, Yutong / Li, Zichuan / Gui, Renyi / Chen, De / Yuan, Mengting / Chai, Yanjun / Shan, Shengdao / Wong, Ming Hung

Chemosphere. 2023 Oct., v. 339 p.139669-

2023

Abstract	Manganese (Mn) is a critical element in soils, essential to plant growth. Long-term and intensively managed Lei bamboo (Phyllostachys violascens) stands are usually subjected to severe soil acidification and Mn activation. However, Mn migration from topsoil to deep soil induced by severe soil acidification was poorly recognized and studied. The distribution and changes of the total and the operationally defined Mn forms in soil profiles and its potential stress and environmental effect were investigated in a chronosequence of Lei bamboo stands (0, 2, 6, 11, and 16 years of stand age). The results showed that the Mn amount was significantly decreased in topsoil and accumulated in subsoil with the long-term and intensive fertilizer application. Soil exchangeable Mn and superphosphate extractable Mn demonstrated large different variation to total Mn, whereas their sum was largely higher than and highly correlated with 8-hydroxyquinoline (HQN) extractable Mn. Soil organic carbon, pH value, exchangeable bases, and soil redox simultaneously controlled soil Mn depletion. In conclusion, long-term and intensive fertilizer application leads to soil acidification and accelerated soil Mn depletion in bamboo stand soil, promoting Mn accumulation in bamboo shoots.
Keywords	8-hydroxyquinoline ; Phyllostachys violascens ; bamboos ; chronosequences ; environmental impact ; fertilizer application ; manganese ; pH ; plant growth ; soil acidification ; soil organic carbon ; stand age ; subsoil ; superphosphate ; topsoil ; China ; Bamboo stand ; Intensive fertilizer application ; Acidification ; Mn losses ; Extractable mn
Language	English
Dates of publication	2023-10
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2023.139669
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 2540: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Comparative studies on the chemical composition and pharmacological effects of vinegar-processed antler glue modified from Lei Gong Pao Zhi Lun and traditional water-processed antler glue.

Shi, Mengqi / Li, Tianshi / Zhao, Yan / He, Zhongmei / Zong, Ying / Chen, Weijia / Du, Rui

Journal of ethnopharmacology

2023 Volume 321, Page(s) 117508

Abstract: ... of preparing antler glue by boiling antlers in vinegar and then concentrating them is recorded in Lei Gong Pao ... method of antler glue is water extraction and concentration. But Lei Gong Pao Zhi Lun differs in Chinese ... Zhi Lun (Master Lei's Discourse on Medicinal Processing). In modern times, the typical processing ...

Abstract	Ethnopharmacological relevance: Antler glue is a classic medicinal to enhance sexual function in traditional Chinese medicine (TCM), which was first recorded in Shen Nong Ben Cao Jing (Shennong's Classic of the Materia Medica). Vinegar-processing is a classic method of processing traditional Chinese medicine. The method of preparing antler glue by boiling antlers in vinegar and then concentrating them is recorded in Lei Gong Pao Zhi Lun (Master Lei's Discourse on Medicinal Processing). In modern times, the typical processing method of antler glue is water extraction and concentration. However, it is not clear whether there is a difference in the effect of these two processing methods on the chemical composition and pharmacological activity of antler glue. Aim of the study: The Chinese Pharmacopoeia (2020) records that the processing method of antler glue is water extraction and concentration. But Lei Gong Pao Zhi Lun differs in Chinese Pharmacopoeia (2020), which records the processing method of vinegar extraction and concentration. The effect of the two processing methods on antler glue's chemical composition and pharmacological activity is unknown. So this study aimed to elucidate the difference between different processing methods on the chemical composition and the treatment effect on oligoasthenospermia of antler glue. Materials and methods: So the automatic amino acid analyzer is used to determine the amino acid content of two different processing methods of antler glue. Proteomics was performed to detect the protein components of two different processing methods of antler glue and analyze them. Cyclophosphamide-induced mice models of oligoasthenospermia were used to study the different pharmacological effects of antler glue in two different processing methods. An automatic sperm analyzer observed the quantity and quality of sperm in mice epididymis. Serum sex hormone testosterone (T), luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels in mice were tested using the enzyme-linked immunosorbent assay (ELISA) kits. Hematoxylin-eosin (H&E) staining was used to analyze pathological alterations in mouse testicular tissue. The transcriptome has been used to reveal the potential mechanism of antler glue in treating oligoasthenospermia. Mitochondrial complex activity assay kits were used to assay the activity of mitochondrial respiratory chain complex I-V in mouse testicular tissue. Western blot was used to determine the expression of related proteins in mouse testicular tissue. Results: Vinegar-processing can increase the alanine, proline, and glycine content in antler glue, reduce the length of protein peptides in antler glue, and produce a variety of unique proteins. Vinegar-processed antler glue (VAG) increased sperm density, sperm survival, sperm viability, and serum sex hormone levels in oligozoospermic mice. It reversed testicular damage caused by cyclophosphamide, and the effects were differently superior to those of water-processed antler glue (WAG). In addition, transcriptomics and related experiments have shown that VAG can increase the expression of Ndufa2, Uqcr11, Cox6b1, and Atp5i genes and proteins in mouse testis, thus promoting adenosine diphosphate (ATP) synthesis by increasing the activity of mitochondrial respiratory chain complexes I, III, IV and V. By promoting the oxidative phosphorylation process to produce more ATP, VAG can achieve the therapeutic effect of oligoasthenospermia. Conclusion: Vinegar-processing method can increase the content of active ingredients in antler glue. VAG increases ATP levels in the testes by promoting the process of oxidative phosphorylation to treat oligozoospermia.
MeSH term(s)	Humans ; Mice ; Male ; Animals ; Antlers/chemistry ; Acetic Acid ; Semen/chemistry ; Proteins ; Gonadal Steroid Hormones ; Oligospermia ; Amino Acids ; Cyclophosphamide ; Adenosine Triphosphate
Chemical Substances	Acetic Acid (Q40Q9N063P) ; Proteins ; Gonadal Steroid Hormones ; Amino Acids ; Cyclophosphamide (8N3DW7272P) ; Adenosine Triphosphate (8L70Q75FXE)
Language	English
Publishing date	2023-12-06
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117508
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1494: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Introducing sedum affects root‐soil interface phytoremediation of heavy metals in lei bamboo forest and potential risks from edible bamboo shoots

Bian, Fangyuan / Zhang, Xiaoping / Zhong, Zheke / Wen, Xing / Xiu, Chengming / Li, Qiaoling / Huang, Zhi-yuan

Land Degradation & Development. 2023 Apr. 15, v. 34, no. 6 p.1820-1829

2023

Abstract: ... is unknown. In this study, lei bamboo and Sedum plumbizincicola were planted as a monoculture and ...

Abstract	Introducing hyperaccumulators in bamboo forests is an efficient method for heavy metals (HMs) pollution control. HMs can be transferred from soil to bamboo and accumulate in edible bamboo shoots. However, how phytoremediation or cropping systems affect root‐soil interface and transference of HMs from soil to bamboo shoots is unknown. In this study, lei bamboo and Sedum plumbizincicola were planted as a monoculture and intercropping system (bamboo/sedum) on contaminated land. Soil properties, rhizosphere bacterial communities, and HMs concentrations in plants and soils were compared among bamboo in monoculture (B), bamboo in intercropping (BI), sedum in intercropping (SI), and sedum in monoculture (S). In BI, the total nitrogen was 10.8% lower, while total organic carbon (TOC) was 12.3% higher than in B; HMs removal amount in BI was higher than in B, especially cadmium. Intercropping increased the HMs concentrations, bioconcentration factor, translocation factor, and hazard quotient of bamboo shoots. Significant differences in bacterial community structures were confirmed by principal coordinate analysis. Gemmatimonadetes, Actinobacteria, Chloroflexi, and Kaistobacter were identified as biomarkers that increased significantly in intercropping, enhancing the diversity of ecological functions in the rhizosphere. Redundancy analysis revealed that rhizosphere bacterial communities were significantly correlated with soil properties, especially TOC and available phosphorus. Intercropping could remarkably alter soil chemical and microbiological properties and facilitate ecosystem restoration by enhancing soil TOC sequestration, activating rhizobacterial community at the root‐soil interface, and finally increasing HMs removal. As the consumption of bamboo shoots grown in contaminated soils may pose health risks, more attention is needed on this issue in phytoremediation studies.
Keywords	Actinobacteria ; Chloroflexi ; Gemmatimonadetes ; Sedum plumbizincicola ; bacterial communities ; bamboos ; bioaccumulation factor ; biomarkers ; cadmium ; ecological restoration ; forests ; hyperaccumulators ; land degradation ; multidimensional scaling ; phosphorus ; phytoremediation ; polluted soils ; rhizosphere ; total nitrogen ; total organic carbon
Language	English
Dates of publication	2023-0415
Size	p. 1820-1829.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	1319202-4
ISSN	1085-3278
ISSN	1085-3278
DOI	10.1002/ldr.4571
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 5058: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Effects of Long-Term Chemical and Organic Fertilizer Application on Soil Phosphorus Fractions in Lei Bamboo Plantations

Qiaoling Li / Aibo Li / Zhiyuan Huang / Zheke Zhong / Fangyuan Bian / Xiaoping Zhang

Sustainability, Vol 14, Iss 15658, p

2022 Volume 15658

Abstract: ... the soil P fractions and associated soil properties in southern Chinese Lei bamboo plantations using ...

Abstract	Phosphorus (P) is a key nutrient in forest ecosystems in subtropical regions. However, the effects of the long-term application of organic amendments on P availability are poorly understood. Here, we compared the soil P fractions and associated soil properties in southern Chinese Lei bamboo plantations using both an intensive management system (IMS) and a traditional management system (TMS). The results show that the IMS significantly ( p < 0.05) increased the soil total organic carbon (C), soil ammonium N (NH 4 -N), total P, and available potassium content; microbial biomass C and P content; P activation coefficient, and soil C:P ratios, while significantly ( p < 0.05) decreasing pH and microbial C:P. The labile-P-to-total-phosphorus-content ratio increased significantly in the IMS (46%) compared with that in the TMS (32%). The selected soil properties (except nitrate [NO 3 -N]) were significantly related to soil P fractions (except for concentrated HCl-extracted organic P). The IMS had a higher C:P ratio and labile P content than the TMS, suggesting that the IMS could promote soil P transformation and availability. Overall, the IMS increased soil P availability and supply capacity, and the changes in P forms could be a risk factor for P loss.
Keywords	bamboo plantations ; forest management ; long-term fertilization ; phosphorus loss ; phosphorus availability ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
Subject code	550
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Book ; Online: Spiking Neural Network Learning, Benchmarking, Programming and Executing

Li, Guoqi / Li, Guoqi / Li, Haizhou / Li, Peng / Neftci, Emre O. / Deng, Lei / Song, Yam

2020

Keywords	Science: general issues ; Neurosciences ; deep spiking neural networks ; SNN learning algorithms ; programming framework ; SNN benchmarks ; neuromorphics
Size	1 electronic resource (234 pages)
Publisher	Frontiers Media SA
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021229998
ISBN	9782889637676 ; 2889637670
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Lei-gong-gen formula granule attenuates hyperlipidemia in rats via cGMP-PKG signaling pathway.

Lan, Taijin / Li, Qiaofeng / Chang, Ming / Yin, Chunli / Zhu, Dan / Wu, Zheng / Li, Xiaolan / Zhang, Weiquan / Yue, Bangwen / Shi, Junlin / Yuan, Hebao / Su, Zhiheng / Guo, Hongwei

Journal of ethnopharmacology

2020 Volume 260, Page(s) 112989

Abstract: Ethnopharmacological relevance: Lei-gong-gen formula granule (LFG) is a folk prescription derived ...

Abstract	Ethnopharmacological relevance: Lei-gong-gen formula granule (LFG) is a folk prescription derived from Zhuang nationality, the largest ethnic minority among the 56 nationalities in China. It is composed of three herbs, namely Centella asiatica (L.) Urb., Eclipta prostrata (L.) L., Smilax glabra Roxb. It has been widely used as health protection tea for many years to prevent cardiovascular and cerebrovascular diseases such as hyperlipidemia and hypertension. Aim of the study: This study validated the lipid-lowering effect of LFG in a hyperlipidemia rat model. Then we employed network pharmacology and molecular biological approach to identify the active ingredients of LFG, corresponding targets, and its anti-hyperlipidemia mechanisms. Materials and methods: Hyperlipidemia rat model was established by feeding male Sprague-Dawley rats with high-fat diet for two weeks. LFG (two doses of 10 and 20 g/kg) was administered orally to hyperlipidemia rat model for 4 weeks, twice per day. Serum levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) were monitored in rats pre and post-treatment. Hematoxylin-eosin staining was applied to observe the pathology and lipid accumulation of liver. We then performed network pharmacology analysis to predict the ingredients, their associated targets, and hyperlipidemia associated targets. Pathway analysis with significant genes was carried out using KEGG pathway. These genes and proteins intersectioned between compound targets and hyperlipidemia targets were further verified with samples from hyperlipidemia rats treated with LFG using Real-time RT-PCR and Western Blot. Results: LFG attenuated hyperlipidemia in rat model, and this was characterized with decreased serum levels of TC, LDL-C, liver wet weight, and liver index. LFG alleviated the hepatic steatosis in hyperlipidemia rats. Network pharmacology analysis identified 53 bioactive ingredients from LFG formula (three herbs), which link to 765 potential targets. 53 hyperlipidemia associated genes were retrieved from public databases. There were 10 common genes between ingredients-targets and hyperlipidemia associated genes, which linked to 20 bioactive ingredients. Among these 10 genes, 3 of them were validated to be involved in LFG's anti-hyperlipidemia effect using Real-time RT-PCR, namely ADRB2 encoding beta-2 adrenergic receptor, NOS3 encoding nitric oxide synthase 3, LDLR encoding low-density lipoprotein receptor. The cGMP-PKG signaling pathway was enriched for hyperlipidemia after pharmacology network analysis with ADRB2, NOS3, and LDLR. Interestingly, expression of cGMP-dependent protein kinase (PKG) was downregulated in hyperlipidemia rat after LFG treatment. Molecular docking study further supported that ferulic acid, histidine, p-hydroxybenzoic acid, and linalool were potential active ingredients for LFG's anti-hyperlipidemia effect. LC-MS/MS analysis confirmed that ferulic acid and p-hydroxybenzoic acid were active ingredients of LFG. Conclusion: LFG exhibited the lipid-lowering effect, which might be attributed to downregulating ADRB2 and NOS3, and upregulating LDLR through the cGMP-PKG signaling pathway in hyperlipidemia rat. Ferulic acid and p-hydroxybenzoic acid might be the underlying active ingredients which affect the potential targets for their anti-hyperlipidemia effect.
MeSH term(s)	Animals ; Centella/chemistry ; Chromatography, Liquid ; Cyclic GMP/metabolism ; Cyclic GMP-Dependent Protein Kinases/metabolism ; Diet, High-Fat ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/pharmacology ; Eclipta/chemistry ; Hyperlipidemias/drug therapy ; Hypolipidemic Agents/administration & dosage ; Hypolipidemic Agents/chemistry ; Hypolipidemic Agents/pharmacology ; Lipids/blood ; Male ; Molecular Docking Simulation ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; Smilax/chemistry ; Tandem Mass Spectrometry
Chemical Substances	Drugs, Chinese Herbal ; Hypolipidemic Agents ; Lipids ; Cyclic GMP-Dependent Protein Kinases (EC 2.7.11.12) ; Cyclic GMP (H2D2X058MU)
Language	English
Publishing date	2020-06-08
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.112989
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1494: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A network pharmacology-based approach to explore the active ingredients and molecular mechanism of Lei-gong-gen formula granule on a spontaneously hypertensive rat model.

Li, Qiaofeng / Lan, Taijin / He, Songhua / Chen, Weiwei / Li, Xiaolan / Zhang, Weiquan / Liu, Ying / Zhang, Qiuping / Chen, Xin / Han, Yaoyao / Su, Zhiheng / Zhu, Dan / Guo, Hongwei

Chinese medicine

2021 Volume 16, Issue 1, Page(s) 99

Abstract: Background: Lei-gong-gen formula granule (LFG) is a folk prescription derived from Zhuang ...

Abstract	Background: Lei-gong-gen formula granule (LFG) is a folk prescription derived from Zhuang nationality, the largest ethnic minority among 56 nationalities in China. It consists of three herbs, namely Eclipta prostrata (L.) L., Smilax glabra Roxb, and Centella asiatica (L.) Urb. It has been widely used as health protection tea for hundreds of years to prevent hypertension in Guangxi Zhuang Autonomous Region. The purpose of this study is to validate the antihypertensive effect of LFG on the spontaneously hypertensive rat (SHR) model, and to further identify the effective components and anti-hypertension mechanism of LFG. Methods: The effects of LFG on blood pressure, body weight, and heart rate were investigated in vivo using the SHR model. The levels of NO, ANG II, and ET-1 in the serum were measured, and pathological changes in the heart were examined by H&E staining. The main active components of LFG, their corresponding targets, and hypertension associated pathways were discerned through network pharmacology analysis based on the Traditional Chinese Medicine Systems Pharmacology (TCMSP), Traditional Chinese Medicine Integrated Database (TCMID), and the Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM). Then the predicted results were further verified by molecular biology experiments such as RT-qPCR and western blot. Additionally, the potential active compounds were predicted by molecular docking technology, and the chemical constituents of LFG were analyzed and identified by UPLC-QTOF/MS technology. Finally, an in vitro assay was performed to investigate the protective effects of potential active compounds against hydrogen peroxide (H Results: LFG could effectively reduce blood pressure and increase serum NO content in SHR model. Histological results showed that LFG could ameliorate pathological changes such as cardiac hypertrophy and interstitial inflammation. From network pharmacology analysis, 53 candidate active compounds of LFG were collected, which linked to 765 potential targets, and 828 hypertension associated targets were retrieved, from which 12 overlapped targets both related to candidate active compounds from LFG and hypertension were screened and used as the potential targets of LFG on antihypertensive effect. The molecular biology experiments of the 12 overlapped targets showed that LFG could upregulate the mRNA and protein expressions of NOS3 and proto-oncogene tyrosine-protein kinase SRC (SRC) in the thoracic aorta. Pathway enrichment analysis showed that the PI3K-AKT signaling pathway was closely related to the expression of NOS3 and SRC. Moreover, western blot results showed that LFG significantly increased the protein expression levels of PI3K and phosphorylated AKT in SHR model, suggesting that LFG may active the PI3K-AKT signaling pathway to decrease hypertension. Molecular docking study further supported that p-hydroxybenzoic acid, cedar acid, shikimic acid, salicylic acid, nicotinic acid, linalool, and histidine can be well binding with NOS3, SRC, PI3K, and AKT. UPLC-QTOF/MS analysis confirmed that p-hydroxybenzoic acid, shikimic acid, salicylic acid, and nicotinic acid existed in LFG. Pre-treatment of HUVEC with nicotinic acid could alleviate the effect on cell viability induced by H Conclusions: LFG can reduce the blood pressure in SHR model, which might be attributed to increasing the NO level in serum for promoting vasodilation via upregulating SRC expression level and activating the PI3K-AKT-NOS3 signaling pathway. Nicotinic acid might be the potential compound for LFG antihypertensive effect.
Language	English
Publishing date	2021-10-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2260322-0
ISSN	1749-8546
ISSN	1749-8546
DOI	10.1186/s13020-021-00507-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

To top

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito