LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 200

Search options

  1. Article ; Online: Hybrid-supervised deep learning for domain transfer 3D protoacoustic image reconstruction.

    Lang, Yankun / Jiang, Zhuoran / Sun, Leshan / Xiang, Liangzhong / Ren, Lei

    Physics in medicine and biology

    2024  Volume 69, Issue 8

    Abstract: ... ...

    Abstract Objective
    MeSH term(s) Male ; Humans ; Protons ; Deep Learning ; Imaging, Three-Dimensional ; Prostate ; Proton Therapy ; Image Processing, Computer-Assisted/methods
    Chemical Substances Protons
    Language English
    Publishing date 2024-04-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 208857-5
    ISSN 1361-6560 ; 0031-9155
    ISSN (online) 1361-6560
    ISSN 0031-9155
    DOI 10.1088/1361-6560/ad3327
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  2. Article: Microbial diversity in camel milk from Xinjiang, China as revealed by metataxonomic analysis.

    Sun, Miao / Shao, Wei / Liu, Zhengyu / Ma, Xianlan / Chen, He / Zheng, Nan / Zhao, Yankun

    Frontiers in microbiology

    2024  Volume 15, Page(s) 1367116

    Abstract: The quality of raw camel milk is affected by its bacterial composition and diversity. However, few studies have investigated the bacterial composition and diversity of raw camel milk. In this study, we obtained 20 samples of camel milk during spring and ... ...

    Abstract The quality of raw camel milk is affected by its bacterial composition and diversity. However, few studies have investigated the bacterial composition and diversity of raw camel milk. In this study, we obtained 20 samples of camel milk during spring and summer in Urumqi and Hami, Xinjiang, China. Single-molecule real-time sequencing technology was used to analyze the bacterial community composition. The results revealed that there were significant seasonal differences in the bacterial composition and diversity of camel milk. Overall,
    Language English
    Publishing date 2024-03-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2024.1367116
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article ; Online: Proteomic Analysis Reveals the Association between the Pathways of Glutathione and α-Linolenic Acid Metabolism and Lanthanum Accumulation in Tea Plants

    Zhihui Xue / Zhidan Chen / Yankun Wang / Weijiang Sun

    Molecules, Vol 28, Iss 1124, p

    2023  Volume 1124

    Abstract: Lanthanum can affect the growth and development of the tea plant. Tieguanyin (TGY) and Shuixian (SX) cultivars of Camellia sinensis were selected to explore the mechanism underlying the accumulation of lanthanum (tea plants’ most accumulated rare earth ... ...

    Abstract Lanthanum can affect the growth and development of the tea plant. Tieguanyin (TGY) and Shuixian (SX) cultivars of Camellia sinensis were selected to explore the mechanism underlying the accumulation of lanthanum (tea plants’ most accumulated rare earth element) through proteomics. Roots and fresh leaves of TGY and SX with low- and high-accumulation potential for lanthanum, respectively, were studied; 845 differentially expressed proteins (DEPs) were identified. Gene ontology analysis showed that DEPs were involved in redox processes and related to molecular functions. Kyoto Encyclopedia of Genes and Genomes metabolic pathway analysis showed that DEPs were associated with glutathione (GSH) and α-linolenic acid metabolism, plant pathogen interaction, and oxidative phosphorylation. Thirty-seven proteins in the GSH metabolism pathway showed significant differences, wherein 18 GSH S-transferases showed differential expression patterns in the root system. Compared with the control, expression ratios of GST (TEA004130.1) and GST (TEA032216.1) in TGY leaves were 6.84 and 4.06, respectively, after lanthanum treatment; these were significantly higher than those in SX leaves. The LOX2.1 (TEA011765.1) and LOX2.1 (TEA011776.1) expression ratios in the α-linolenic acid metabolic pathway were 2.44 and 6.43, respectively, in TGY roots, which were significantly higher than those in SX roots. The synthesis of specific substances induces lanthanum-associated defense responses in TGY, which is of great significance for plant yield stability.
    Keywords rare earth elements ; lanthanum ; genotypes ; Tieguanyin ; Shuixian ; Camellia sinensis ; Organic chemistry ; QD241-441
    Subject code 580
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  4. Article ; Online: An Approach for Measuring Complexity Degree of International Engineering Projects

    Qianqian Ju / Yankun Sun / Ran Chen

    Sustainability, Vol 15, Iss 9791, p

    2023  Volume 9791

    Abstract: With the increasing trend of globalization, countries actively join the international engineering market, increasing the complexity of projects. An appropriate method for assessing project complexity can help project managers recognize the current ... ...

    Abstract With the increasing trend of globalization, countries actively join the international engineering market, increasing the complexity of projects. An appropriate method for assessing project complexity can help project managers recognize the current situation and solve problems. However, existing complexity studies ignored the contribution of human element. The impact of human activities on the complexity of socio-economic systems is concerned in the Harmony Management Theory (HMT), therefore, this study proposed a complexity measurement based on the Harmony Management Theory. Firstly, an evaluation indicator system including three dimensions of organizational, technological, and environmental complexity is proposed through a literature review, Back-Propagation Neural Network-Decision Making Trial and Evaluation Laboratory (BP-DEMATEL) method, and Interval-valued intuitionistic Fuzzy Sets (IIFS) are used to calculate complexity scores. Then, a case study of Yawan high-speed railway project is conducted to verify the effectiveness of the proposed method. The main conclusions are as follows: (1) The complexity measurement based on harmony theory can be carried out from three aspects: Organization, technology, and environment. (2) The overall complexity performance of case project is 0.52, showing a medium level. (3) Technical complexity is the most important factor of the case project. The method proposed in this study can identify the influencing factors of complexity and calculate the comprehensive evaluation value of complexity. Based on the final quantified results, managers can formulate appropriate measures and match the project with appropriate resources so as to improve the performance of International Engineering Projects (IEPs).
    Keywords international engineering projects (IEPs) ; IEPs complexity (CIEPs) ; CIEPs measurement ; project management ; Harmony Management Theory (HMT) ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
    Subject code 690
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  5. Article ; Online: Proteomic Analysis Reveals the Association between the Pathways of Glutathione and α-Linolenic Acid Metabolism and Lanthanum Accumulation in Tea Plants.

    Xue, Zhihui / Chen, Zhidan / Wang, Yankun / Sun, Weijiang

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 3

    Abstract: Lanthanum can affect the growth and development of the tea plant. Tieguanyin (TGY) and Shuixian (SX) cultivars of Camellia sinensis were selected to explore the mechanism underlying the accumulation of lanthanum (tea plants' most accumulated rare earth ... ...

    Abstract Lanthanum can affect the growth and development of the tea plant. Tieguanyin (TGY) and Shuixian (SX) cultivars of Camellia sinensis were selected to explore the mechanism underlying the accumulation of lanthanum (tea plants' most accumulated rare earth element) through proteomics. Roots and fresh leaves of TGY and SX with low- and high-accumulation potential for lanthanum, respectively, were studied; 845 differentially expressed proteins (DEPs) were identified. Gene ontology analysis showed that DEPs were involved in redox processes and related to molecular functions. Kyoto Encyclopedia of Genes and Genomes metabolic pathway analysis showed that DEPs were associated with glutathione (GSH) and α-linolenic acid metabolism, plant pathogen interaction, and oxidative phosphorylation. Thirty-seven proteins in the GSH metabolism pathway showed significant differences, wherein 18 GSH S-transferases showed differential expression patterns in the root system. Compared with the control, expression ratios of GST (TEA004130.1) and GST (TEA032216.1) in TGY leaves were 6.84 and 4.06, respectively, after lanthanum treatment; these were significantly higher than those in SX leaves. The LOX2.1 (TEA011765.1) and LOX2.1 (TEA011776.1) expression ratios in the α-linolenic acid metabolic pathway were 2.44 and 6.43, respectively, in TGY roots, which were significantly higher than those in SX roots. The synthesis of specific substances induces lanthanum-associated defense responses in TGY, which is of great significance for plant yield stability.
    MeSH term(s) Camellia sinensis/metabolism ; Lanthanum ; alpha-Linolenic Acid/metabolism ; Proteomics ; Plant Leaves/metabolism ; Gene Expression Regulation, Plant ; Tea/metabolism ; Plant Proteins/genetics ; Plant Proteins/metabolism
    Chemical Substances Lanthanum (6I3K30563S) ; alpha-Linolenic Acid (0RBV727H71) ; Tea ; Plant Proteins
    Language English
    Publishing date 2023-01-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28031124
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article: The Effect of Nutrient Deficiencies on the Annual Yield and Root Growth of Summer Corn in a Double-Cropping System.

    Wang, Chuangyun / Ma, Yankun / Zhao, Rong / Sun, Zheng / Wang, Xiaofen / Gao, Fei

    Plants (Basel, Switzerland)

    2024  Volume 13, Issue 5

    Abstract: The North China Plain has a typical winter wheat-summer corn double-cropping pattern. The effects of nutrient deficiency conditions on the root characteristics and yield of summer corn in the double-cropping system were studied for four years. Long-term ... ...

    Abstract The North China Plain has a typical winter wheat-summer corn double-cropping pattern. The effects of nutrient deficiency conditions on the root characteristics and yield of summer corn in the double-cropping system were studied for four years. Long-term monotonous fertilization patterns undermine crop rotation systems and are detrimental to the sustainability of agricultural production. To complement the development of rational fertilization strategies by exploring the response of crop rotation systems to nutrient deficiencies, an experiment was conducted in a randomized complete block design consisting of five treatments with three replicates for each treatment: (1) an adequate supply of nitrogen and phosphate fertilizers and potash-deficient treatment (T1); (2) an adequate supply of nitrogen and potash fertilizers and phosphorus-deficient treatment (T2); (3) an adequate supply of phosphorus and potash fertilizers and nitrogen-deficient treatment (T3); (4) nutrient-sufficient treatment for crop growth (T4); and (5) no-fertilizer treatment (CK). The results showed that different nutrient treatments had significant effects on the root length density (RLD), root surface area density (RSAD), and root dry weight density (RDWD) in summer corn. At the physiological maturity stage (R6), the root indexes of RLD, RSAD, and RDWD were significantly higher in the 0-20 cm soil layer in T4 compared to CK, with an increase of 86.2%, 131.4%, and 100.0%, respectively. Similarly, in the 20-40 cm soil layer, the root indexes of T4 were 85.7%, 61.3%, and 50.0% higher than CK, with varied differences observed in the other nutrient-deficient treatments. However, there was no significant difference among the treatments in the 40-60 cm layer except for T4, whose root index showed a difference. The root fresh weight and root dry matter in T4, T3, T2, and T1 were increased to different degrees compared with CK. In addition, these differences in root indexes affected the annual yield of crops, which increased by 20.96%, 21.95%, and 8.14% in T4, T2, and T1, respectively, compared to CK. The spike number and the number of grains per spike of T4 were 10.8% and 8.3% higher than those of CK, which led to the differences in summer corn yields. The 1000-kernel weight of T4, T2, and T1 were 9.5%, 8.8%, and 7.4% higher than that of CK, whereas the determining nutrient was nitrogen fertilizer, and phosphorus fertilizer had a higher effect on yield than potassium fertilizer. This provides a theoretical basis for the effect of nutrient deficiency conditions on yield stability in a double-cropping system.
    Language English
    Publishing date 2024-02-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants13050682
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article ; Online: Hybrid-Supervised Deep Learning for Domain Transfer 3D Protoacoustic Image Reconstruction.

    Lang, Yankun / Jiang, Zhuoran / Sun, Leshan / Xiang, Liangzhong / Ren, Lei

    ArXiv

    2023  

    Abstract: Protoacoustic imaging showed great promise in providing real-time 3D dose verification of proton therapy. However, the limited acquisition angle in protoacoustic imaging induces severe artifacts, which significantly impairs its accuracy for dose ... ...

    Abstract Protoacoustic imaging showed great promise in providing real-time 3D dose verification of proton therapy. However, the limited acquisition angle in protoacoustic imaging induces severe artifacts, which significantly impairs its accuracy for dose verification. In this study, we developed a deep learning method with a Recon- Enhance two-stage strategy for protoacoustic imaging to address the limited view issue. Specifically, in the Recon-stage, a transformer-based network was developed to reconstruct initial pressure maps from radiofrequency signals. The network is trained in a hybrid-supervised approach, where it is first trained using supervision by the iteratively reconstructed pressure map and then fine-tuned using transfer learning and self-supervision based on the data fidelity constraint. In the Enhance-stage, a 3D U-net is applied to further enhance the image quality with supervision from the ground truth pressure map. The final protoacoustic images are then converted to dose for proton verification. The results evaluated on a dataset of 126 prostate cancer patients achieved an average RMSE of 0.0292, and an average SSIM of 0.9618, significantly out-performing related start-of-the-art methods. Qualitative results also demonstrated that our approach addressed the limit-view issue with more details reconstructed. Dose verification achieved an average RMSE of 0.018, and an average SSIM of 0.9891. Gamma index evaluation demonstrated a high agreement (94.7% and 95.7% for 1%/3 mm and 1%/5 mm) between the predicted and the ground truth dose maps. Notably, the processing time was reduced to 6 seconds, demonstrating its feasibility for online 3D dose verification for prostate proton therapy.
    Language English
    Publishing date 2023-08-11
    Publishing country United States
    Document type Preprint
    ISSN 2331-8422
    ISSN (online) 2331-8422
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  8. Article ; Online: Versatile Tetrasilane for Time-Controlled Palladium-Catalyzed Divergent Synthesis of Silacycles via C-H Activation.

    Xu, Yankun / Sun, Mingjie / Xu, Weiwei / Deng, Guobo / Liang, Yun / Yang, Yuan

    Journal of the American Chemical Society

    2023  Volume 145, Issue 28, Page(s) 15303–15312

    Abstract: Transition-metal-catalyzed intermolecular annulation of silicon reagents with organic molecules is still underdeveloped due to the scarcity of silicon reagent types and their diverse reactivity. Herein, a readily accessible silicon reagent (octamethyl-1, ... ...

    Abstract Transition-metal-catalyzed intermolecular annulation of silicon reagents with organic molecules is still underdeveloped due to the scarcity of silicon reagent types and their diverse reactivity. Herein, a readily accessible silicon reagent (octamethyl-1,4-dioxacyclohexasilane) has been developed for divergent synthesis of silacycles via time-controlled palladium-catalyzed cascade C-H silacyclization. This protocol enables the rapid and selective transformation of acrylamides into spirosilacycles with different ring sizes, including benzodioxatetrasilecines, benzooxadisilepines, and benzosiloles, in moderate to good yields through a time switch. Notably, the tetrasilane reagent can also be utilized for C-H silacyclization of 2-halo-
    Language English
    Publishing date 2023-07-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.3c02875
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article ; Online: High-throughput process development from gene cloning to protein production.

    Sun, Manman / Gao, Alex Xiong / Liu, Xiuxia / Yang, Yankun / Ledesma-Amaro, Rodrigo / Bai, Zhonghu

    Microbial cell factories

    2023  Volume 22, Issue 1, Page(s) 182

    Abstract: In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for ...

    Abstract In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for a given application is also increasing. Faced with peer competition, budgetary, and time constraints, companies and laboratories must find ways to develop a robust manufacturing process for recombinant protein production. In this review, we explore high-throughput technologies for recombinant protein expression and present a holistic high-throughput process development strategy that spans from genes to proteins. We discuss the challenges that come with this task, the limitations of previous studies, and future research directions.
    MeSH term(s) Cloning, Molecular ; Amino Acid Sequence ; Genomics ; Laboratories ; Recombinant Proteins/genetics
    Chemical Substances Recombinant Proteins
    Language English
    Publishing date 2023-09-15
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-023-02184-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article ; Online: SHMT2 promotes papillary thyroid cancer metastasis through epigenetic activation of AKT signaling.

    Sun, Min / Zhao, Mingjian / Li, Ruowen / Zhang, Yankun / Shi, Xiaojia / Ding, Changyuan / Ma, Chunhong / Lu, Jinghui / Yue, Xuetian

    Cell death & disease

    2024  Volume 15, Issue 1, Page(s) 87

    Abstract: Cancer cells alter their metabolism and epigenetics to support cancer progression. However, very few modulators connecting metabolism and epigenetics have been uncovered. Here, we reveal that serine hydroxymethyltransferase-2 (SHMT2) generates S- ... ...

    Abstract Cancer cells alter their metabolism and epigenetics to support cancer progression. However, very few modulators connecting metabolism and epigenetics have been uncovered. Here, we reveal that serine hydroxymethyltransferase-2 (SHMT2) generates S-adenosylmethionine (SAM) to epigenetically repress phosphatase and tensin homolog (PTEN), leading to papillary thyroid cancer (PTC) metastasis depending on activation of AKT signaling. SHMT2 is elevated in PTC, and is associated with poor prognosis. Overexpressed SHMT2 promotes PTC metastasis both in vitro and in vivo. Proteomic enrichment analysis shows that AKT signaling is activated, and is positively associated with SHMT2 in PTC specimens. Blocking AKT activation eliminates the effects of SHMT2 on promoting PTC metastasis. Furthermore, SHMT2 expression is negatively associated with PTEN, a negative AKT regulator, in PTC specimens. Mechanistically, SHMT2 catalyzes serine metabolism and produces activated one-carbon units that can generate SAM for the methylation of CpG islands in PTEN promoter for PTEN suppression and following AKT activation. Importantly, interference with PTEN expression affects SHMT2 function by promoting AKT signaling activation and PTC metastasis. Collectively, our research demonstrates that SHMT2 connects metabolic reprogramming and epigenetics, contributing to the poor progression of PTC.
    MeSH term(s) Humans ; Thyroid Cancer, Papillary/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Thyroid Neoplasms/metabolism ; Proteomics ; Epigenesis, Genetic ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Cell Line, Tumor
    Chemical Substances Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
    Language English
    Publishing date 2024-01-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2541626-1
    ISSN 2041-4889 ; 2041-4889
    ISSN (online) 2041-4889
    ISSN 2041-4889
    DOI 10.1038/s41419-024-06476-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top