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Article ; Online: Protocol for xenotransplantation of human skin and streptozotocin diabetes induction in immunodeficient mice to study impaired wound healing.

Li, Zhuqing / Sumpio, Brandon / Wang, Enya / Contreras, Mauricio / Mezghani, Ikram / Theocharidis, Georgios / Veves, Aristidis

STAR protocols

2023 Volume 4, Issue 1, Page(s) 102029

Abstract: Here, we present a protocol for the integration of human skin onto the backs of diabetic immunodeficient mice, providing a versatile in vivo model for mimicking and studying mechanisms involved in impaired cutaneous wound healing. This protocol includes ... ...

Abstract	Here, we present a protocol for the integration of human skin onto the backs of diabetic immunodeficient mice, providing a versatile in vivo model for mimicking and studying mechanisms involved in impaired cutaneous wound healing. This protocol includes instructions for the grafting of human skin, induction of diabetes using streptozotocin and wounding/post-wounding care of immunodeficient mice, as well as suggested downstream tissue analyses. This preclinical mouse model can be used to validate the efficacy of newly developed wound dressings. For complete details on the use and execution of this protocol, please refer to Theocharidis et al. (2022).
MeSH term(s)	Humans ; Mice ; Animals ; Diabetes Mellitus, Experimental ; Wound Healing ; Transplantation, Heterologous ; Skin ; Streptozocin/toxicity
Chemical Substances	Streptozocin (5W494URQ81)
Language	English
Publishing date	2023-01-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2022.102029
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Article: Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow.

Shalaby, S Y / Chitragari, G / Sumpio, B J / Sumpio, B E

The International journal of angiology : official publication of the International College of Angiology, Inc

2017 Volume 26, Issue 2, Page(s) 109–115

Abstract: Extracellular signal-regulated kinase 5 (ERK5) has been reported to regulate endothelial integrity and protect from vascular dysfunction under laminar flow. Previously reported research indicates that under laminar flow ERK5 is activated with production ... ...

Abstract	Extracellular signal-regulated kinase 5 (ERK5) has been reported to regulate endothelial integrity and protect from vascular dysfunction under laminar flow. Previously reported research indicates that under laminar flow ERK5 is activated with production of atheroprotective molecules. However, the characterization of ERK5 activation and levels under different flow patterns has not been investigated. Confluent HUVECs were serum-starved then seeded on glass slides. HUVECs incubated in 1% FBS were exposed to continuous laminar flow (CLF), to-and-fro flow (TFF), or pulsatile forward flow (PFF) in a parallel plate flow chamber. At the end of experimentation, cell lysates were immunoblotted with antibodies to phospho-ERK5 and total ERK5. ERK5 activation was assessed by the levels of phosphorylated ERK5. The densitometric mean ± SEM is calculated and analyzed by ANOVA.
Language	English
Publishing date	2017-02-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	1144390-x
ISSN	1061-1711
ISSN	1061-1711
DOI	10.1055/s-0037-1599057
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Article: Use of Serum Protein Measurements as Biomarkers that Can Predict the Outcome of Diabetic Foot Ulceration.

Theocharidis, Georgios / Sumpio, Brandon / Wang, Enya / Mezghani, Ikram / Giurini, John M / Kalavros, Nikolaos / Valsami, Eleftheria-Angeliki / Vlachos, Ioannis / Heydarpour, Mahyar / Veves, Aristidis

Advances in wound care

2024

Abstract: Objectives: ...

Abstract	Objectives:
Language	English
Publishing date	2024-02-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2650541-1
ISSN	2162-1934 ; 2162-1918
ISSN (online)	2162-1934
ISSN	2162-1918
DOI	10.1089/wound.2023.0126
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Article ; Online: Popliteal Artery Occlusion with Collateral Blood Flow in a Reducible Knee Dislocation During Pregnancy: A Case Report.

Schneble, Christopher A / Kahan, Joseph B / Burroughs, Patrick J / Nasreddine, Adam Y / Sumpio, Bauer E / Medvecky, Michael J

JBJS case connector

2021 Volume 11, Issue 2

Abstract: Case: A 29-year-old healthy woman, 19 weeks pregnant, sustained a right posterolateral knee dislocation with multiligamentous injury and a complete occlusive injury to the right popliteal artery yet had adequate distal perfusion. She declined operative ... ...

Abstract	Case: A 29-year-old healthy woman, 19 weeks pregnant, sustained a right posterolateral knee dislocation with multiligamentous injury and a complete occlusive injury to the right popliteal artery yet had adequate distal perfusion. She declined operative management for both the knee dislocation and the arterial injury, and successful collaboration between obstetrical, vascular, and orthopaedic surgical services resulted in limb preservation and restoration of function. Conclusion: This is a unique case of traumatic complete popliteal artery occlusion with adequate collateral arterial perfusion after a reducible posterolateral knee dislocation in a pregnant patient that resulted in limb preservation with nonoperative management.
MeSH term(s)	Adult ; Female ; Humans ; Joint Dislocations/complications ; Knee Dislocation/complications ; Knee Dislocation/surgery ; Popliteal Artery/injuries ; Popliteal Artery/surgery ; Pregnancy ; Vascular Surgical Procedures/adverse effects ; Vascular System Injuries/etiology
Language	English
Publishing date	2021-04-09
Publishing country	United States
Document type	Case Reports ; Journal Article
ISSN	2160-3251
ISSN (online)	2160-3251
DOI	e20.00516
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Article: Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow

Shalaby, S. Y. / Chitragari, G. / Sumpio, B. J. / Sumpio, B. E.

International Journal of Angiology

2017 Volume 26, Issue 02, Page(s) 109–115

Abstract	Extracellular signal-regulated kinase 5 (ERK5) has been reported to regulate endothelial integrity and protect from vascular dysfunction under laminar flow. Previously reported research indicates that under laminar flow ERK5 is activated with production of atheroprotective molecules. However, the characterization of ERK5 activation and levels under different flow patterns has not been investigated. Confluent HUVECs were serum-starved then seeded on glass slides. HUVECs incubated in 1% FBS were exposed to continuous laminar flow (CLF), to-and-fro flow (TFF), or pulsatile forward flow (PFF) in a parallel plate flow chamber. At the end of experimentation, cell lysates were immunoblotted with antibodies to phospho-ERK5 and total ERK5. ERK5 activation was assessed by the levels of phosphorylated ERK5. The densitometric mean ± SEM is calculated and analyzed by ANOVA. p < 0.05 is considered significant. Levels of ERK5 decreased with all flow conditions with the largest decrease in TFF flow condition. TFF and CLF exhibited sustained ERK5 phosphorylation in HUVECs stimulated for up to 4 hours. PFF had transient phosphorylation of ERK5 at 2 hours, which then became undetectable at 4 hours of exposure to flow. Also, TFF and CLF both showed decreased levels at 4 hours, suggesting a decrease in activation for these flow conditions. Exposure of HUVEC to different types of shear stress results in varying patterns of activation of ERK5. Activation of ERK5 with TFF suggests a role in the pathogenesis of atherosclerosis and vascular remodeling under disturbed flow conditions.
Keywords	atherosclerosis ; shear stress ; extracellular signal-regulated kinase 5 ; slowing of progression of atherosclerosis ; atheroprotective molecules ; vascular dysfunction ; disturbed flow
Language	English
Publishing date	2017-02-25
Publisher	Thieme Medical Publishers
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	1144390-x
ISSN	1615-5939 ; 1061-1711
ISSN (online)	1615-5939
ISSN	1061-1711
DOI	10.1055/s-0037-1599057
Database	Thieme publisher's database

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Zs.A 3635: Show issues

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Article ; Online: Prosthetic options available for the diabetic lower limb amputee.

Chitragari, Gautham / Mahler, David B / Sumpio, Brandon J / Blume, Peter A / Sumpio, Bauer E

Clinics in podiatric medicine and surgery

2014 Volume 31, Issue 1, Page(s) 173–185

Abstract: Although the rate of lower limb amputation in patients with diabetes is decreasing, amputation still remains a major complication of diabetes. Prosthetics have been long used to help amputees ambulate. The last decade has seen many advances in prostheses ...

Abstract	Although the rate of lower limb amputation in patients with diabetes is decreasing, amputation still remains a major complication of diabetes. Prosthetics have been long used to help amputees ambulate. The last decade has seen many advances in prostheses with the enhanced understanding of the mechanics of ambulation and improved use of technology. This review describes the different types of prosthetic options available for below knee, ankle, and foot amputees, emphasizing the latest advances in prosthetic design.
MeSH term(s)	Amputation ; Artificial Limbs ; Diabetic Foot/surgery ; Humans ; Leg ; Prosthesis Design
Language	English
Publishing date	2014-01
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1323674-x
ISSN	1558-2302 ; 0742-0668 ; 0891-8422
ISSN (online)	1558-2302
ISSN	0742-0668 ; 0891-8422
DOI	10.1016/j.cpm.2013.09.008
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Article ; Online: A comparison of immediate postoperative rigid and soft dressings for below-knee amputations.

Sumpio, Brandon / Shine, Sarah R / Mahler, David / Sumpio, Bauer E

Annals of vascular surgery

2013 Volume 27, Issue 6, Page(s) 774–780

Abstract: Background: A variety of postsurgical dressings are used after a below-knee amputation (BKA), but there is no evidence-based guideline on which dressing to use. The purpose of this study was to compare the application of immediate postoperative rigid ... ...

Abstract	Background: A variety of postsurgical dressings are used after a below-knee amputation (BKA), but there is no evidence-based guideline on which dressing to use. The purpose of this study was to compare the application of immediate postoperative rigid dressings or soft dressings on the healing times of BKA. Methods: In this retrospective analysis we compared 151 patients who underwent BKA from 2000 to 2012 at Yale New Haven Hospital. Patient demographics were collected and the type of postoperative dressing utilized was recorded. Ninety-one patients received a rigid plaster or plastic dressing and 60 received a soft gauze dressing with knee immobilizer. Time was measured between amputation and initial casting of prosthesis. Results: Average age for the rigid dressing group was 58.6 years, with 78.2% presenting with diabetes mellitus, compared with the soft dressing group of 61.0 years, with 82.8%, respectively. Using a Kaplan-Meier analysis, patients fit with a rigid dressing demonstrated significantly decreased healing time from BKA to initial casting for a prosthesis (P = 0.02). After the first 60 days, 58.24% of patients who received a rigid dressing were ready to be cast, compared with 38.33% of patients receiving a soft dressing (P = 0.03). Conclusions: Below-knee amputees had a significantly quicker healing time, as measured by the time to be cast for prosthesis, when a rigid dressing was used compared with a soft dressing. Consideration should be given to the use of a rigid dressing after BKA to expedite healing and promote earlier ambulation.
MeSH term(s)	Amputees/rehabilitation ; Bandages/utilization ; Equipment Design ; Follow-Up Studies ; Humans ; Knee ; Postoperative Care/methods ; Postoperative Period ; Retrospective Studies ; Tibia/surgery ; Wound Healing
Language	English
Publishing date	2013-08
Publishing country	Netherlands
Document type	Comparative Study ; Journal Article
ZDB-ID	1027366-9
ISSN	1615-5947 ; 0890-5096
ISSN (online)	1615-5947
ISSN	0890-5096
DOI	10.1016/j.avsg.2013.03.007
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Zs.A 2882: Show issues

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Article: Foot ulcers.

Sumpio, B E

The New England journal of medicine

2000 Volume 343, Issue 11, Page(s) 787–793

Abstract: The role of the primary care physician in the evaluation, diagnosis, and management of pedal wounds is critical (Table 2). Patient education is essential for the reduction of risk factors and for the early recognition of foot complications. Careful ... ...

Abstract	The role of the primary care physician in the evaluation, diagnosis, and management of pedal wounds is critical (Table 2). Patient education is essential for the reduction of risk factors and for the early recognition of foot complications. Careful assessment of vascular disease (leading to bypass surgery when indicated), evaluation and management of biomechanical abnormalities, and aggressive treatment of any infections are also required. Optimal management can reduce the number of major amputations, prevent infection, decrease the probability of ulceration, maintain skin integrity, and improve function. The multidisciplinary approach will provide a comprehensive treatment protocol that will increase the long-term viability of the foot.
MeSH term(s)	Biomechanical Phenomena ; Diabetes Complications ; Foot/blood supply ; Foot Deformities/complications ; Foot Deformities/therapy ; Foot Ulcer/etiology ; Foot Ulcer/physiopathology ; Foot Ulcer/prevention & control ; Foot Ulcer/therapy ; Gait/physiology ; Humans ; Ischemia/complications ; Nervous System Diseases/complications ; Risk Factors ; Wound Infection/therapy
Language	English
Publishing date	2000-09-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. ; Review
ZDB-ID	207154-x
ISSN	1533-4406 ; 0028-4793
ISSN (online)	1533-4406
ISSN	0028-4793
DOI	10.1056/NEJM200009143431107
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Article ; Online: A Pilot Study of a Standardized Smoking Cessation Intervention for Patients with Vascular Disease.

Rajaee, Sareh / Holder, Tara / Indes, Jeff E / Muhs, Bart / Sarac, Timur / Sumpio, Bauer / Toll, Benjamin A / Ochoa Chaar, Cassius I

Annals of vascular surgery

2019 Volume 61, Page(s) 91–99.e3

Abstract: Background: The goal of this study is to evaluate the efficacy of a smoking cessation intervention performed by a vascular surgery provider compared with current smoking cessation practices.: Methods: Patients with peripheral arterial and aneurysmal ... ...

Abstract	Background: The goal of this study is to evaluate the efficacy of a smoking cessation intervention performed by a vascular surgery provider compared with current smoking cessation practices. Methods: Patients with peripheral arterial and aneurysmal disease who presented to the vascular surgery service at a tertiary care center over a 9-month period were randomized to either control or intervention group. Both control and intervention groups received 2 weeks of free nicotine patches and referral to an outpatient smoking-cessation program. The intervention group additionally received a brief presentation by a vascular surgeon regarding the benefits of smoking cessation, with a focus on vascular complications. At enrollment and at follow-up, patients underwent carbon monoxide breath testing and completed a survey. The primary outcome was smoking cessation or reduction among control and intervention groups in patients who underwent medical management, endovascular procedures, or open surgical procedures. Fisher's exact test was used to assess the primary outcome among groups. Results: Fifty-nine patients were enrolled in the trial initially, but 55 had 1-month follow-up (control n = 28, intervention n = 27) and 52 had long-term follow-up (control n = 28, intervention n = 24). By long-term follow-up, 40 patients (77%) had reduced smoking by at least 50% and 16 patients (31%) had quit completely. At long-term follow-up, 88% of patients in the intervention group and 68% of patients in the control group reduced smoking (P = 0.1). Conclusions: A large proportion of vascular patients who received 2 weeks of nicotine replacement with or without the addition of brief smoking cessation counseling delivered by a vascular surgery provider were able to reduce smoking and maintain reduction after 6 months. Delivery of a brief standardized smoking cessation counseling session by a vascular surgery provider is safe and feasible. Additional randomized controlled trials with large enrollment periods and long follow-up are needed to determine the efficacy of this intervention in comparison to standard care.
MeSH term(s)	Aneurysm/diagnosis ; Aneurysm/physiopathology ; Aneurysm/therapy ; Cardiovascular Agents/therapeutic use ; Cholinergic Agents/administration & dosage ; Cholinergic Agents/adverse effects ; Connecticut ; Endovascular Procedures ; Female ; Humans ; Male ; Middle Aged ; Nicotine/administration & dosage ; Nicotine/adverse effects ; Patient Education as Topic ; Peripheral Arterial Disease/diagnosis ; Peripheral Arterial Disease/physiopathology ; Peripheral Arterial Disease/therapy ; Pilot Projects ; Risk Factors ; Risk Reduction Behavior ; Smokers ; Smoking/adverse effects ; Smoking Cessation/methods ; Time Factors ; Tobacco Use Cessation Devices/adverse effects ; Transdermal Patch ; Treatment Outcome ; Vascular Surgical Procedures
Chemical Substances	Cardiovascular Agents ; Cholinergic Agents ; Nicotine (6M3C89ZY6R)
Language	English
Publishing date	2019-08-23
Publishing country	Netherlands
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	1027366-9
ISSN	1615-5947 ; 0890-5096
ISSN (online)	1615-5947
ISSN	0890-5096
DOI	10.1016/j.avsg.2019.06.017
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Article: Review of prevalence and outcome of vascular disease in patients with diabetes mellitus.

Weiss, J S / Sumpio, B E

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery

2006 Volume 31, Issue 2, Page(s) 143–150

Abstract: Objectives: Review the literature to determine the prevalence and outcome in patients with diabetes that undergo surgery to correct carotid artery stenosis, lower extremity arterial disease, and abdominal aortic aneurysm (AAA).: Design and materials: ...

Abstract	Objectives: Review the literature to determine the prevalence and outcome in patients with diabetes that undergo surgery to correct carotid artery stenosis, lower extremity arterial disease, and abdominal aortic aneurysm (AAA). Design and materials: Studies were obtained from searches over the past 15 years on the National Library of Medicine's online search engine. Results: The review demonstrated an equivalent prevalence of carotid artery stenosis requiring surgery in patients with diabetes, it favored no increase risk of post-CEA stroke, and it was split on perioperative morbidity and mortality risk. There was an increase prevalence of lower extremity arterial disease requiring surgery in patients with diabetes, it favored equivalent patency and limb salvage rates, and it was split on the morbidity and mortality risk. The review demonstrated a decrease in AAA prevalence among patients with diabetes, it found an increase in the morbidity risk, and equivalent mortality risk. Conclusions: Stroke, graft patency, and limb salvage rates in patients with diabetes after surgery are similar to patients without diabetes; however, their risk of complications is increased after surgery and the mortality risk may be higher after CEA.
MeSH term(s)	Aortic Aneurysm, Abdominal/complications ; Aortic Aneurysm, Abdominal/surgery ; Atherosclerosis/complications ; Carotid Stenosis/complications ; Carotid Stenosis/surgery ; Diabetes Complications ; Diabetic Angiopathies/surgery ; Endarterectomy, Carotid ; Humans ; Leg/blood supply ; Peripheral Vascular Diseases/complications ; Peripheral Vascular Diseases/surgery ; Stroke/etiology
Language	English
Publishing date	2006-02
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1225869-6
ISSN	1532-2165 ; 1078-5884
ISSN (online)	1532-2165
ISSN	1078-5884
DOI	10.1016/j.ejvs.2005.08.015
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