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  1. Article ; Online: A Comparison Study of the Detection Limit of Omicron SARS-CoV-2 Nucleocapsid by Various Rapid Antigen Tests.

    Dobrynin, Daniela / Polischuk, Iryna / Pokroy, Boaz

    Biosensors

    2022  Volume 12, Issue 12

    Abstract: Rapid antigen tests (RATs) are widely used worldwide to detect SARS-CoV-2 since they are an easy-to-use kit and offer rapid results. The RAT detects the presence of the nucleocapsid protein, which is located inside the virus. However, the sensitivity of ... ...

    Abstract Rapid antigen tests (RATs) are widely used worldwide to detect SARS-CoV-2 since they are an easy-to-use kit and offer rapid results. The RAT detects the presence of the nucleocapsid protein, which is located inside the virus. However, the sensitivity of the different RATs varies between commercially available kits. The test result might change due to various factors, such as the variant type, infection date, swab's surface, the manner in which one performs the testing and the mucus components. Here, we compare the detection limit of seven commercially available RATs by introducing them to known SARS-CoV-2 nucleocapsid protein amounts from the Omicron variant. It allows us to determine the detection limit, disregarding the influences of other factors. A lower detection limit of the RAT is necessary since earlier detection will help reduce the spread of the virus and allow faster treatment, which might be crucial for the population at risk.
    Language English
    Publishing date 2022-11-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios12121083
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Online: A Comparison Study of the Detection Limit of Omicron SARS-CoV-2 Nucleocapsid by various Rapid Antigen Tests

    Dobrynin, Daniela / Polishchuk, Iryna / Pokroy, Boaz

    2022  

    Abstract: Since the first case of COVID-19 disease in Wuhan in December 2019, there is a worldwide struggle to reduce the transmission of acute respiratory syndrome coronavirus SARS-CoV-2. Many countries worldwide decided to impose local lockdowns in order to ... ...

    Abstract Since the first case of COVID-19 disease in Wuhan in December 2019, there is a worldwide struggle to reduce the transmission of acute respiratory syndrome coronavirus SARS-CoV-2. Many countries worldwide decided to impose local lockdowns in order to reduce person-to-person interactions, masks became obligatory especially in closed spaces, and there was a general requirement for social distance. However, the most efficient method to reduce continuing spreading of infection among the population, and in the meantime maintain a regular daily life, is early detection of infected contagious people. Up to now, the most reliable method for SARS-CoV-2 detection is reverse-transcriptase PCR test (RT-PCR). It is possible to detect the virus even if there is only one RNA strand in the sample, and run hundreds of samples simultaneously. This method has a few disadvantages, such as high cost, is time consuming, the need for medical laboratories and skilled staff to perform the test, and the major flaw: the lack of appropriate number of available tests. The latterly prominent Omicron variant (B.1.1.529) and its derivatives have caused a tremendous increase in the number of infected people due to its enhanced transmissibility. This all emphasizes the high demand for easy-to-use, cheap and available detection tests.
    Keywords Quantitative Biology - Other Quantitative Biology
    Subject code 630
    Publishing date 2022-07-29
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  3. Article ; Online: Adsorption of SARS CoV-2 spike proteins on various functionalized surfaces correlates with the high transmissibility of Delta and Omicron variants.

    Dobrynin, Daniela / Polishchuk, Iryna / Portal, Lotan / Zlotver, Ivan / Sosnik, Alejandro / Pokroy, Boaz

    Materials today. Bio

    2022  Volume 14, Page(s) 100265

    Abstract: The SARS-CoV-2 virus emerged at the end of 2019 and rapidly developed several mutated variants, specifically the Delta and Omicron, which demonstrate higher transmissibility and escalating infection cases worldwide. The dominant transmission pathway of ... ...

    Abstract The SARS-CoV-2 virus emerged at the end of 2019 and rapidly developed several mutated variants, specifically the Delta and Omicron, which demonstrate higher transmissibility and escalating infection cases worldwide. The dominant transmission pathway of this virus is via human-to-human contact and aerosols which once inhaled interact with the mucosal tissue, but another possible route is through contact with surfaces contaminated with SARS-CoV-2, often exhibiting long-term survival. Here we compare the adsorption capacities of the S1 and S2 subunits of the spike (S) protein from the original variant to that of the S1 subunit from the Delta and Omicron variants on self-assembled monolayers by Quartz Crystal ​Microbalance. The results clearly show a significant difference in adsorption capacity between the different variants, as well as between the S1 and S2 subunits. Overall, our study demonstrates that while the Omicron variant is able to adsorb much more successfully than the Delta, both variants show enhanced adsorption capacity than that of the original strain. We also examined the influence of pH conditions on the adsorption ability of the S1 subunit and found that adsorption was strongest at pH 7.4, which is the physiological pH. The main conclusion of this study is that there is a strong correlation between the adsorption capacity and the transmissibility of the various SARS-CoV-2 variants.
    Language English
    Publishing date 2022-04-19
    Publishing country England
    Document type Journal Article
    ISSN 2590-0064
    ISSN (online) 2590-0064
    DOI 10.1016/j.mtbio.2022.100265
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Adsorption of SARS CoV-2 spike proteins on various functionalized surfaces correlates with the high transmissibility of Delta and Omicron variants

    Daniela Dobrynin / Iryna Polishchuk / Lotan Portal / Ivan Zlotver / Alejandro Sosnik / Boaz Pokroy

    Materials Today Bio, Vol 14, Iss , Pp 100265- (2022)

    2022  

    Abstract: The SARS-CoV-2 virus emerged at the end of 2019 and rapidly developed several mutated variants, specifically the Delta and Omicron, which demonstrate higher transmissibility and escalating infection cases worldwide. The dominant transmission pathway of ... ...

    Abstract The SARS-CoV-2 virus emerged at the end of 2019 and rapidly developed several mutated variants, specifically the Delta and Omicron, which demonstrate higher transmissibility and escalating infection cases worldwide. The dominant transmission pathway of this virus is via human-to-human contact and aerosols which once inhaled interact with the mucosal tissue, but another possible route is through contact with surfaces contaminated with SARS-CoV-2, often exhibiting long-term survival. Here we compare the adsorption capacities of the S1 and S2 subunits of the spike (S) protein from the original variant to that of the S1 subunit from the Delta and Omicron variants on self-assembled monolayers by Quartz Crystal Microbalance. The results clearly show a significant difference in adsorption capacity between the different variants, as well as between the S1 and S2 subunits. Overall, our study demonstrates that while the Omicron variant is able to adsorb much more successfully than the Delta, both variants show enhanced adsorption capacity than that of the original strain. We also examined the influence of pH conditions on the adsorption ability of the S1 subunit and found that adsorption was strongest at pH 7.4, which is the physiological pH. The main conclusion of this study is that there is a strong correlation between the adsorption capacity and the transmissibility of the various SARS-CoV-2 variants.
    Keywords SARS-CoV-2 ; Spike protein ; COVID-19 ; Omicron variant ; Delta variant ; Quartz crystal microbalance ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  5. Book ; Online: Adsorption of SARS CoV-2 Spike Proteins on Various Functionalized Surfaces Correlates with the Strong Infectivity of Delta and Omicron Variants

    Dobrynin, Daniela / Polishchuk, Iryna / Portal, Lotan / Zlotver, Ivan / Sosnik, Alejandro / Pokroy, Boaz

    2022  

    Abstract: The SARS CoV-2 virus emerged at the end of 2019 and rapidly developed several mutated variants, specifically the Delta and Omicron, which demonstrate higher infectivity and escalating infection cases worldwide. The dominant transmission pathway of this ... ...

    Abstract The SARS CoV-2 virus emerged at the end of 2019 and rapidly developed several mutated variants, specifically the Delta and Omicron, which demonstrate higher infectivity and escalating infection cases worldwide. The dominant transmission pathway of this virus is via human-to-human contact and aerosols, but another possible route is through contact with surfaces contaminated with SARS-CoV-2, often exhibiting long-period survival. Here we compare the adsorption capacities of the S1 and S2 subunits of the spike (S) protein from the original variant to that of the S1 subunit from the Delta and Omicron variants. The results clearly show a significant difference in adsorption capacity between the different variants, as well as between the S1 and S2 subunits. Overall, our study demonstrates that while the Omicron variant is able to adsorb much more successfully than the Delta, both variants show enhanced adsorption capacity than the original strain. We also examined the influence of pH conditions on the adsorption ability of the S1 subunit and found that adsorption was strongest at pH 7.4, which is the physiological pH. The main conclusion of this study is that there is a strong correlation between the adsorption capacity and the infectivity of the various SARS CoV-2 variants.
    Keywords Physics - Biological Physics ; Condensed Matter - Materials Science ; Quantitative Biology - Biomolecules
    Subject code 572
    Publishing date 2022-02-24
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Surface-Initiated Passing-Through Polymerization on a Rubber Substrate: Supplying Monomer from Swollen Substrates

    McDermott, Sean T. / Ward, Shawn P. / Vy, Ngoc Chau H. / Wang, Zilu / Morales-Acosta, Mayra Daniela / Dobrynin, Andrey V. / Adamson, Douglas H.

    Macromolecules. 2022 Aug. 05, v. 55, no. 16

    2022  

    Abstract: Passing-through polymerization is a technique for growing surface-initiated polymer brushes where monomers are supplied to the growing chains by diffusing through the initiator-functionalized surface. This inverts the monomer concentration gradient ... ...

    Abstract Passing-through polymerization is a technique for growing surface-initiated polymer brushes where monomers are supplied to the growing chains by diffusing through the initiator-functionalized surface. This inverts the monomer concentration gradient relative to conventional grafting-from mechanisms, allowing for the synthesis of thicker and more densely packed brushes. Here we use a combination of coarse-grained computer simulations and experimental techniques to demonstrate how this approach can be implemented, with swellable network-like substrates supplying monomers to the functionalized surface as they diffuse out of the swollen network. Compared to grafting-from polymer brushes, this method shows a higher contact angle and a greater brush mass. Additionally, we observed that the passing-through approach could lead to strain-induced crystallization in the brush layer or wrinkling of the brush surface, which we used to estimate the brush thickness. The developed method opens a path for large-scale synthesis of the brush-modified elastic substrates.
    Keywords computers ; contact angle ; crystallization ; polymerization ; polymers ; rubber
    Language English
    Dates of publication 2022-0805
    Size p. 7265-7272.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1491942-4
    ISSN 1520-5835 ; 0024-9297
    ISSN (online) 1520-5835
    ISSN 0024-9297
    DOI 10.1021/acs.macromol.2c00685
    Database NAL-Catalogue (AGRICOLA)

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