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  1. Article: Palmitic Amide Triggers Virus Life Cycle

    Zhang, Xinyi / Zhuang, Jianjian / Huang, Liquan / Zhang, Xiaobo

    Frontiers in microbiology

    2022  Volume 13, Page(s) 924533

    Abstract: Viruses contribute to the mortality of organisms, consequentially altering biological species composition of an ecosystem and having a threat on human health. As the most famous model for the initiation of virus infection, the Hershey-Chase experiment ... ...

    Abstract Viruses contribute to the mortality of organisms, consequentially altering biological species composition of an ecosystem and having a threat on human health. As the most famous model for the initiation of virus infection, the Hershey-Chase experiment has revealed that on infection, the bacteriophage genomic DNA is injected into its host bacterium, while the viral capsid is left on the outer membrane of host cell. However, little is known about the injection of any other materials into the cytoplasm of host cells along with genomic DNA to trigger the virus life cycle. In this study, the results showed that palmitic amide packaged in the virions of GVE2, a bacteriophage infecting deep-sea hydrothermal vent thermophile
    Language English
    Publishing date 2022-06-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.924533
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Blue light induces ROS mediated apoptosis and degradation of AML1-ETO oncoprotein in Kasumi-1 cells.

    Zhuang, Jianjian / Xia, Liping / Zou, Zheyu / Yin, Juxin

    Medical oncology (Northwood, London, England)

    2022  Volume 39, Issue 5, Page(s) 52

    Abstract: Light-emitting diode (LED)-based therapies, particularly blue LEDs with wavelengths of 400-500 nm, have shown beneficial results in several cancers, including melanoma, lymphoid cells, and skin tumors. In this study, the cell viability and apoptosis of ... ...

    Abstract Light-emitting diode (LED)-based therapies, particularly blue LEDs with wavelengths of 400-500 nm, have shown beneficial results in several cancers, including melanoma, lymphoid cells, and skin tumors. In this study, the cell viability and apoptosis of Kasumi-1 cells treated by blue light (BL) irradiation have been explored. Firstly, BL can specially inhibit the proliferation and promote the apoptosis of Kasumi-1 cells. Furthermore, the apoptosis was triggered by the production of reactive oxygen species and the decline of mitochondrial membrane potential which was regulated by the ratio of Bcl-2(Bcl-xL)/Bax; BL caused the cells' final apoptosis accompanied with the increased cleavage of caspase-3 and poly-ADP-ribose polymerase. Finally, BL induced the degradation of AML1-ETO dependent on the activation of caspase-3. These results are helpful for establishing a low toxicity and high efficiency strategy of BL irradiation for clinical treatment of Kasumi-1 cells.
    MeSH term(s) Apoptosis/radiation effects ; Caspase 3/metabolism ; Cell Line, Tumor ; Cell Survival/radiation effects ; Color ; Core Binding Factor Alpha 2 Subunit/metabolism ; Core Binding Factor Alpha 2 Subunit/radiation effects ; Humans ; Membrane Potential, Mitochondrial/radiation effects ; Oncogene Proteins, Fusion/metabolism ; Oncogene Proteins, Fusion/radiation effects ; Photic Stimulation/methods ; Poly(ADP-ribose) Polymerases/metabolism ; RUNX1 Translocation Partner 1 Protein/metabolism ; RUNX1 Translocation Partner 1 Protein/radiation effects ; Reactive Oxygen Species/radiation effects
    Chemical Substances AML1-ETO fusion protein, human ; Core Binding Factor Alpha 2 Subunit ; Oncogene Proteins, Fusion ; RUNX1 Translocation Partner 1 Protein ; Reactive Oxygen Species ; Poly(ADP-ribose) Polymerases (EC 2.4.2.30) ; Caspase 3 (EC 3.4.22.-)
    Language English
    Publishing date 2022-02-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1201189-7
    ISSN 1559-131X ; 0736-0118 ; 1357-0560
    ISSN (online) 1559-131X
    ISSN 0736-0118 ; 1357-0560
    DOI 10.1007/s12032-022-01650-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1899: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  3. Article ; Online: Adsorption-Free Self-Priming Direct Digital Dual-crRNA CRISPR/Cas12a-Assisted Chip for Ultrasensitive Detection of Pathogens.

    Xia, Liping / Yin, Juxin / Zhuang, Jianjian / Yin, Weihong / Zou, Zheyu / Mu, Ying

    Analytical chemistry

    2023  Volume 95, Issue 10, Page(s) 4744–4752

    Abstract: Rapid and sensitive pathogen detection methods are critical for disease diagnosis and treatment. RPA-CRISPR/Cas12 systems have displayed remarkable potential in pathogen detection. A self-priming digital PCR chip is a powerful and attractive tool for ... ...

    Abstract Rapid and sensitive pathogen detection methods are critical for disease diagnosis and treatment. RPA-CRISPR/Cas12 systems have displayed remarkable potential in pathogen detection. A self-priming digital PCR chip is a powerful and attractive tool for nucleic detection. However, the application of the RPA-CRISPR/Cas12 system to the self-priming chip still has great challenges due to the problems of protein adsorption and two-step detection mode of RPA-CRISPR/Cas12. In this study, an adsorption-free self-priming digital chip was developed and a direct digital dual-crRNAs (3D) assay was established based on the chip for ultrasensitive detection of pathogens. This 3D assay combined the advantages of rapid amplification of RPA, specific cleavage of Cas12a, accurate quantification of digital PCR, and point-of-care testing (POCT) of microfluidics, enabling accurate and reliable digital absolute quantification of
    MeSH term(s) CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems ; Adsorption ; Biological Assay ; Cell Nucleus ; Nucleic Acid Amplification Techniques
    Chemical Substances RNA, Guide, CRISPR-Cas Systems
    Language English
    Publishing date 2023-03-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05560
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Digital Recombinase Polymerase Amplification, Digital Loop-Mediated Isothermal Amplification, and Digital CRISPR-Cas Assisted Assay: Current Status, Challenges, and Perspectives.

    Yin, Weihong / Zhuang, Jianjian / Li, Jiale / Xia, Liping / Hu, Kai / Yin, Juxin / Mu, Ying

    Small (Weinheim an der Bergstrasse, Germany)

    2023  Volume 19, Issue 49, Page(s) e2303398

    Abstract: Digital nucleic acid detection based on microfluidics technology can quantify the initial amount of nucleic acid in the sample with low equipment requirements and simple operations, which can be widely used in clinical and in vitro diagnosis. Recently, ... ...

    Abstract Digital nucleic acid detection based on microfluidics technology can quantify the initial amount of nucleic acid in the sample with low equipment requirements and simple operations, which can be widely used in clinical and in vitro diagnosis. Recently, isothermal amplification technologies such as recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats-CRISPR associated proteins (CRISPR-Cas) assisted technologies have become a hot spot of attention and state-of-the-art digital nucleic acid chips have provided a powerful tool for these technologies. Herein, isothermal amplification technologies including RPA, LAMP, and CRISPR-Cas assisted methods, based on digital nucleic acid microfluidics chips recently, have been reviewed. Moreover, the challenges of digital isothermal amplification and possible strategies to address them are discussed. Finally, future directions of digital isothermal amplification technology, such as microfluidic chip and device manufacturing, multiplex detection, and one-pot detection, are outlined.
    MeSH term(s) Recombinases ; CRISPR-Cas Systems/genetics ; Biological Assay ; Nucleic Acids ; Nucleic Acid Amplification Techniques
    Chemical Substances Recombinases ; Nucleic Acids
    Language English
    Publishing date 2023-08-23
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2168935-0
    ISSN 1613-6829 ; 1613-6810
    ISSN (online) 1613-6829
    ISSN 1613-6810
    DOI 10.1002/smll.202303398
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Ginseng of different ages is affected by the accumulation of heavy metals in ginseng soil.

    Yin, Juxin / Zhuang, Jianjian / Zhang, Xin / Xu, Chaojian / Lv, Shaowu

    PloS one

    2022  Volume 17, Issue 6, Page(s) e0269238

    Abstract: Heavy-metal pollution has been established to affect ginseng quality. However, this effect is still unknown in ginseng of different ages, emphasizing the need to investigate the effects of heavy metals in soils on ginseng growth. Herein, we determined ... ...

    Abstract Heavy-metal pollution has been established to affect ginseng quality. However, this effect is still unknown in ginseng of different ages, emphasizing the need to investigate the effects of heavy metals in soils on ginseng growth. Herein, we determined the content of heavy metals (Cu, Cd, Pb, Hg, and As) in ginseng of different ages (2 to 6-year-old) and the corresponding soil samples. Then, the total ginsenosides content of ginseng and rate-limiting enzyme (HMGR, SQE, CYP450) activity in the synthesis of ginsenosides were assessed. Results from 200 differently-aged Chinese ginseng showed that increased ginsenoside content in 3 to 5-year-old ginseng was paralleled by increased heavy metal element content in ginseng and its soil. The activity of rate-limiting enzymes increased in the first four years of ginseng growth and then exhibited a steady or downward trend. Further analysis suggested that heavy metal elements in soils could directly affect ginsenoside content. Moreover, we found that Cu significantly affected the rate-limiting enzyme CYP450 activity. Further principal component analysis and correlation analysis found that heavy metals could obviously inhibit ginseng growth during the 5th and 6th years. Heavy metal content in soils has huge prospects for predicting ginsenoside, Cu and As content in ginseng. This study provided support for ginseng cultivation, quality research and quality assessment.
    MeSH term(s) China ; Environmental Monitoring ; Ginsenosides/analysis ; Metals, Heavy/analysis ; Panax ; Risk Assessment ; Soil ; Soil Pollutants/analysis
    Chemical Substances Ginsenosides ; Metals, Heavy ; Soil ; Soil Pollutants
    Language English
    Publishing date 2022-06-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0269238
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  6. Article ; Online: A direct and multiplex digital PCR chip for EGFR mutation.

    Yin, Juxin / Xia, Liping / Zou, Zheyu / Zhuang, Jianjian / Mu, Ying

    Talanta

    2022  Volume 250, Page(s) 123725

    Abstract: Digital PCR is a sensitive detection method, which has important applicability in liquid biopsy through the measurement of ctDNA. However, the current sample pre-processing of ctDNA and the multiplex detection capability of digital PCR have limitations. ... ...

    Abstract Digital PCR is a sensitive detection method, which has important applicability in liquid biopsy through the measurement of ctDNA. However, the current sample pre-processing of ctDNA and the multiplex detection capability of digital PCR have limitations. In view of the above two aspects, we developed a digital PCR chip with multiplex capability and established a direct amplification detection method without nucleic acid extraction. Through the design and processing of the chip, we established a self-priming multiplex digital PCR chip, which can detect 4 targets using single fluorescence. This method can be applied to most digital PCR chips. In addition, we used the plasma of lung cancer patients to establish a direct digital PCR detection method based on the chip, thereby avoiding disadvantages caused by the ctDNA extraction process. As a proof of concept, we prepared blood plasma samples with different concentration of ctDNA to prove the chip's multiplex detection capabilities and the results suggested that this multiplex digital PCR is accurate. Overall, our platform provides a novel and promising option for the detection of ctDNA.
    MeSH term(s) Circulating Tumor DNA/genetics ; ErbB Receptors/genetics ; Humans ; Lung Neoplasms/diagnosis ; Lung Neoplasms/genetics ; Multiplex Polymerase Chain Reaction/methods ; Mutation
    Chemical Substances Circulating Tumor DNA ; EGFR protein, human (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1)
    Language English
    Publishing date 2022-07-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2022.123725
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  7. Article: The Long and the Short of Current Nanomedicines for Treating Alzheimer's Disease.

    Gong, Baofeng / Zhuang, Jianhua / Ji, Wenbo / Chen, Xiaohan / Li, Peng / Cheng, Wenbin / Chu, Jianjian / Liang, Wendanqi / He, Bin / Gao, Jie / Yin, You

    Journal of translational internal medicine

    2023  Volume 10, Issue 4, Page(s) 294–296

    Language English
    Publishing date 2023-01-13
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2861892-0
    ISSN 2224-4018 ; 2450-131X
    ISSN (online) 2224-4018
    ISSN 2450-131X
    DOI 10.2478/jtim-2021-0054
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Hydrogel: a Promising New Technique for Treating Alzheimer's Disease.

    Gong, Baofeng / Cheng, Wenbin / Ji, Wenbo / Chen, Xiaohan / Chu, Jianjian / Liang, Wendanqi / He, Bin / Zhuang, Jianhua / Yin, You / Gao, Jie

    Journal of translational internal medicine

    2022  Volume 10, Issue 1, Page(s) 15–17

    Language English
    Publishing date 2022-03-26
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2861892-0
    ISSN 2224-4018 ; 2450-131X
    ISSN (online) 2224-4018
    ISSN 2450-131X
    DOI 10.2478/jtim-2022-0008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Adsorption-Free Self-Priming Direct Digital Dual-crRNA CRISPR/Cas12a-Assisted Chip for Ultrasensitive Detection of Pathogens

    Xia, Liping / Yin, Juxin / Zhuang, Jianjian / Yin, Weihong / Zou, Zheyu / Mu, Ying

    Analytical Chemistry. 2023 Mar. 03, v. 95, no. 10 p.4744-4752

    2023  

    Abstract: Rapid and sensitive pathogen detection methods are critical for disease diagnosis and treatment. RPA-CRISPR/Cas12 systems have displayed remarkable potential in pathogen detection. A self-priming digital PCR chip is a powerful and attractive tool for ... ...

    Abstract Rapid and sensitive pathogen detection methods are critical for disease diagnosis and treatment. RPA-CRISPR/Cas12 systems have displayed remarkable potential in pathogen detection. A self-priming digital PCR chip is a powerful and attractive tool for nucleic detection. However, the application of the RPA-CRISPR/Cas12 system to the self-priming chip still has great challenges due to the problems of protein adsorption and two-step detection mode of RPA-CRISPR/Cas12. In this study, an adsorption-free self-priming digital chip was developed and a direct digital dual-crRNAs (3D) assay was established based on the chip for ultrasensitive detection of pathogens. This 3D assay combined the advantages of rapid amplification of RPA, specific cleavage of Cas12a, accurate quantification of digital PCR, and point-of-care testing (POCT) of microfluidics, enabling accurate and reliable digital absolute quantification of Salmonella in POCT. Our method can provide a good linear relationship of Salmonella detection in the range from 2.58 × 10¹ to 2.58 × 10⁴ cells/mL with a limit of detection ∼0.2 cells/mL within 30 min in a digital chip by targeting the invA gene of Salmonella. Moreover, the assay could directly detect Salmonella in milk without nucleic acid extraction. Therefore, the 3D assay has the significant potential to provide accurate and rapid pathogen detection in POCT. This study provides a powerful nucleic detection platform and facilitates the application of CRISPR/Cas-assisted detection and microfluidic chips.
    Keywords Salmonella ; adsorption ; analytical chemistry ; detection limit ; disease diagnosis ; genes ; microbial detection ; microfluidic technology ; milk ; nucleic acids ; point-of-care systems
    Language English
    Dates of publication 2023-0303
    Size p. 4744-4752.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05560
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    In stock of ZB MED Bonn / Germany

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  10. Article: 2-[(4-Hydroxybenzyl) Amino] Phenol (HBAP) Restores the Mutated p53 to the Level Similar to That of Wild-Type p53 Protein and Inhibits Breast Cancer Growth

    Xu, Chenxi / Zhuang, Jianjian / Zhang, Xiaobo

    Frontiers in cell and developmental biology

    2020  Volume 8, Page(s) 574799

    Abstract: P53 is a transcriptional factor that plays important roles in apoptosis and is mutated in more than 50% of tumor cells. However, the restoration of mutated p53 to the level similar to wild-type p53 by a natural compound has not been explored intensively. ...

    Abstract P53 is a transcriptional factor that plays important roles in apoptosis and is mutated in more than 50% of tumor cells. However, the restoration of mutated p53 to the level similar to wild-type p53 by a natural compound has not been explored intensively. In this study, the 2-[(4-hydroxybenzyl) amino] phenol (HBAP) compound, obtained from deep-sea virus-challenged thermophile
    Language English
    Publishing date 2020-11-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2020.574799
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