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Article ; Online: The Healing Capability of Clove Flower Extract (CFE) in Streptozotocin-Induced (STZ-Induced) Diabetic Rat Wounds Infected with Multidrug Resistant Bacteria.

Ali, Rewaa / Khamis, Tarek / Enan, Gamal / El-Didamony, Gamal / Sitohy, Basel / Abdel-Fattah, Gamal

2022 Volume 27, Issue 7

Abstract: Treatment of diabetic foot ulcer (DFU) is of great challenge as it is shown to be infected by multidrug resistant bacteria (MDR bacteria). Sixty four bacterial isolates were isolated from DFU cases; antibiotic susceptibility tests were carried out for ... ...

Abstract	Treatment of diabetic foot ulcer (DFU) is of great challenge as it is shown to be infected by multidrug resistant bacteria (MDR bacteria). Sixty four bacterial isolates were isolated from DFU cases; antibiotic susceptibility tests were carried out for all of them. One bacterial isolate (number 11) was shown to resist the action of 8 out of 12 antibiotics used and was identified by both a Vitek-2 system and 16S rRNA fingerprints as belonging to
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacteria/genetics ; Diabetes Mellitus, Experimental ; Diabetic Foot/drug therapy ; Diabetic Foot/microbiology ; Drug Resistance, Bacterial ; Epidermal Growth Factor/metabolism ; Plant Extracts/therapeutic use ; RNA, Ribosomal, 16S ; Rats ; Rats, Sprague-Dawley ; Streptozocin ; Syzygium/chemistry ; Syzygium/drug effects ; Wound Healing ; bcl-2-Associated X Protein
Chemical Substances	Anti-Bacterial Agents ; Plant Extracts ; RNA, Ribosomal, 16S ; bcl-2-Associated X Protein ; clove flower extract ; Streptozocin (5W494URQ81) ; Epidermal Growth Factor (62229-50-9)
Language	English
Publishing date	2022-03-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules27072270
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Article ; Online: Hyaluronic acid production by Klebsiella pneumoniae strain H15 (OP354286) under different fermentation conditions.

Ahmed, Rania M / Enan, Gamal / Saed, Safaa / Askora, Ahmed

BMC microbiology

2023 Volume 23, Issue 1, Page(s) 295

Abstract: Background: Hyaluronic acid (HA) has gained significant attention due to its unique physical, chemical, and biological properties, making it widely used in various industries. This study aimed to screen bacterial isolates for HA production, characterize ...

Abstract	Background: Hyaluronic acid (HA) has gained significant attention due to its unique physical, chemical, and biological properties, making it widely used in various industries. This study aimed to screen bacterial isolates for HA production, characterize favorable fermentation conditions, and evaluate the inhibitory effect of bacterial HA on cancer cell lines. Results: A total of 108 bacterial isolates from diverse sources were screened for HA production using HPLC, turbidimetric, and carbazole determination methods. Among the HA-producing isolates, Klebsiella pneumoniae H15 isolated from an animal feces sample, was superior in HA production. The strain was characterized based on its morphological, cultural, and biochemical characteristics. Molecular identification using 16S rDNA sequencing and phylogenetic analysis confirmed its identity. Fermentation conditions, including pH, temperature, time, and agitation rate, were optimized to maximize HA production. The basal medium, comprising sucrose (7.0%) as carbon source and combined yeast extract with peptone (1.25% each) as nitrogen substrate, favored the highest HA production at pH 8.0, for 30 h, at 30 °C, under shaking at 180 rpm. The average maximized HA concentration reached 1.5 g L Conclusions: K. pneumoniae H15, isolated from animal feces demonstrated promising potential for HA production. The most favorable fermentation conditions led to a high HA production. The inhibitory effect of bacterial HA on cancer cell lines highlights its potential therapeutic applications. These findings contribute to a broader understanding and utilization of HA in various industries and therapeutic applications.
MeSH term(s)	Animals ; Fermentation ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/metabolism ; Hyaluronic Acid/metabolism ; Phylogeny ; Culture Media/chemistry
Chemical Substances	Hyaluronic Acid (9004-61-9) ; Culture Media
Language	English
Publishing date	2023-10-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2041505-9
ISSN	1471-2180 ; 1471-2180
ISSN (online)	1471-2180
ISSN	1471-2180
DOI	10.1186/s12866-023-03035-0
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Article: Gasdermin D: A potential mediator and prognostic marker of bladder cancer.

El-Gamal, Randa / Abdelrahim, Mona / El-Sherbiny, Mohamed / Enan, Eman T / El-Nablaway, Mohammad

Frontiers in molecular biosciences

2022 Volume 9, Page(s) 972087

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-09-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2022.972087
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Article ; Online: The Healing Capability of Clove Flower Extract (CFE) in Streptozotocin-Induced (STZ-Induced) Diabetic Rat Wounds Infected with Multidrug Resistant Bacteria

Rewaa Ali / Tarek Khamis / Gamal Enan / Gamal El-Didamony / Basel Sitohy / Gamal Abdel-Fattah

Molecules, Vol 27, Iss 2270, p

2022 Volume 2270

Abstract	Treatment of diabetic foot ulcer (DFU) is of great challenge as it is shown to be infected by multidrug resistant bacteria (MDR bacteria). Sixty four bacterial isolates were isolated from DFU cases; antibiotic susceptibility tests were carried out for all of them. One bacterial isolate (number 11) was shown to resist the action of 8 out of 12 antibiotics used and was identified by both a Vitek-2 system and 16S rRNA fingerprints as belonging to Proteus mirabilis, and was designated Proteus mirabilis LC587231 ( P. mirabilis ). Clove flower extract (CFE) inhibited distinctively the P. mirabilis bacterium obtained. GC-MS spectroscopy showed that this CFE contained nine bioactive compounds. The effect of CFE on wound healing of Type 1 diabetic albino rats ( Rattus norvegicus ) was studied. The results indicated that topical application of CFE hydrogel improved wound size, wound index, mRNA expression of the wound healing markers ( Coli1, MMP9 , Fibronectin , PCNA , and TGFβ ), growth factor signaling pathways ( PPAR-α , PGC1-α , GLP-1 , GLPr-1 , EGF-β , EGF-βr , VEGF-β , and FGF-β ), inflammatory cytokine expression ( IL8 , TNFα , NFKβ , IL1β , and MCP1 ), as well as anti-inflammatory cytokines ( IL4 & IL10 ), pro-apoptotic markers ( FAS , FAS-L , BAX , BAX/BCL-2 , Caspase-3 , P53 , P38 ), as well as an antiapoptotic one ( BCL2 ). Furthermore, it improved the wound oxidative state and reduced the wound microbial load, as the cefepime therapy improved the wound healing parameters. Based on the previous notions, it could be concluded that CFE represents a valid antibiotics alternative for DFU therapy since it improves diabetic wound healing and exerts antibacterial activity either in vitro or in vivo.
Keywords	diabetic foot ulcer ; MDR- Proteus mirabilis ; inflammatory markers ; Syzygium aromaticum ; growth factor ; Organic chemistry ; QD241-441
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Inhibitory action of three lactic acid bacteria cultures on some food-borne pathogens during pickling of green olive fruits.

Almohammadi, Abdul-Raouf / Abdel-Shafi, Seham / Tartour, Eman / Enan, Gamal

Heliyon

2022 Volume 8, Issue 11, Page(s) e11693

Abstract: The healthy olive pickles are those ones which are made by using lactic acid bacteria (LAB) as starter and protective cultures. Three lactic acid bacteria (LAB) strains namely: ...

Abstract	The healthy olive pickles are those ones which are made by using lactic acid bacteria (LAB) as starter and protective cultures. Three lactic acid bacteria (LAB) strains namely:
Language	English
Publishing date	2022-11-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2022.e11693
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Article: Controlling bacterial biofilm formation by native and methylated lupine 11S globulins.

Enan, Gamal / Abdel-Shafi, Seham / El-Nemr, Mona / Shehab, Wesam / Osman, Ali / Sitohy, Mahmoud / Sitohy, Basel

Frontiers in microbiology

2023 Volume 14, Page(s) 1259334

Abstract: The antibacterial and anti-biofilm activities of the 11S globulins isolated from lupin seeds ( ...

Abstract	The antibacterial and anti-biofilm activities of the 11S globulins isolated from lupin seeds (
Language	English
Publishing date	2023-09-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1259334
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Article ; Online: Mapping pathogenic bacteria resistance against common antibiotics and their potential susceptibility to methylated white kidney bean protein.

Sitohy, Mahmoud / Enan, Gamal / Abdel-Shafi, Seham / El-Wafa, Neveen Abou / El-Gazzar, Nashwa / Osman, Ali / Sitohy, Basel

BMC microbiology

2024 Volume 24, Issue 1, Page(s) 49

Abstract: As antibiotics cannot inhibit multidrug-resistant bacteria (MDR), continuous research is mandatory to find other antibacterials from natural resources. Native legume proteins and their modified forms exhibited broad spectra of high antimicrobial ... ...

Abstract	As antibiotics cannot inhibit multidrug-resistant bacteria (MDR), continuous research is mandatory to find other antibacterials from natural resources. Native legume proteins and their modified forms exhibited broad spectra of high antimicrobial activities. Sixteen bacterial isolates were mapped for antibiotic resistance, showing resistance in the range of (58-92%) and (42-92%) in the case of the Gram-negative and Gram-positive bacteria, respectively. White native Phaseolus vulgaris protein (NPP) was isolated from the seeds and methylated (MPP). The MIC range of MPP against 7 MDR bacteria was 10-25 times lower than NPP and could (1 MIC) considerably inhibit their 24 h liquid growth. MPP showed higher antibacterial effectiveness than Gentamycin, the most effective antibiotic against Gram-positive bacteria and the second most effective against Gram-negative bacteria. However, MPP recorded MICs against the seven studied MDR bacteria in the 1-20 µg/mL range, the same for Gentamycin. The combination of Gentamycin and MPP produced synergistic effects against the seven bacteria studied, as confirmed by the Transmission Electron Microscopic images. The antimicrobial activity of MPP against the seven MDR bacteria remained stable after two years of cold storage at 8-10 °C as contrasted to Gentamycin, which lost 20-72% of its antimicrobial effectiveness.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Phaseolus ; Bacterial Infections/drug therapy ; Bacteria ; Drug Resistance, Multiple, Bacterial ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Gentamicins/pharmacology ; Microbial Sensitivity Tests
Chemical Substances	Anti-Bacterial Agents ; Gentamicins
Language	English
Publishing date	2024-02-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2041505-9
ISSN	1471-2180 ; 1471-2180
ISSN (online)	1471-2180
ISSN	1471-2180
DOI	10.1186/s12866-024-03202-x
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Article ; Online: Inhibitory action of three lactic acid bacteria cultures on some food-borne pathogens during pickling of green olive fruits

Abdul-Raouf Almohammadi / Seham Abdel-Shafi / Eman Tartour / Gamal Enan

Heliyon, Vol 8, Iss 11, Pp e11693- (2022)

2022

Abstract: The healthy olive pickles are those ones which are made by using lactic acid bacteria (LAB) as starter and protective cultures. Three lactic acid bacteria (LAB) strains namely: Lactobacillus plantarum LPS10 (L. plantarum), Lactobacillus fermentum PP17 (L. ...

Abstract	The healthy olive pickles are those ones which are made by using lactic acid bacteria (LAB) as starter and protective cultures. Three lactic acid bacteria (LAB) strains namely: Lactobacillus plantarum LPS10 (L. plantarum), Lactobacillus fermentum PP17 (L. fermentum) and Pediococcus acidilactici MH512904 (P. acidilactici) were used for inhibition of some food-borne bacterial pathogens such as Listeria monocytogenes LMG10470 (L. monocytogenes), Staphylococcus aureus ATCC25923 (S. aureus), Bacillus cereus ATCC14579 (B. cereus) and Escherichia coli ATCC25922 (E. coli) in Brain Heart Infusion Broth (BHIB) and during pickling of green olive fruits. Cell free supernatants (CFS) from LAB showed distinctive inhibition of the indicator bacterial pathogens used and the inhibitory activity was more pronounced against Gram positive bacteria than that found against Gram negative E. coli strain used; the inhibitory activity of CFS was more pronounced than that obtained by neutralized cell free supernatants (NCFS). Cultures of LAB were used for inhibition of the food-borne pathogens during pickling of green olive fruits. The food-borne bacterial cells grew in olive pickles brine (control) and their growth (CFU/mL) decreased in treated pickles samples and distinctive differences in growth values (CFU/mL) were observed between control and treated samples. CFS from L. plantarum affected target cells of both L. monocytogenes and E. coli and caused cell deformations, cell shrinkage and cell lysis as showed by Transmission Electron Microscopic (TEM) examinations.
Keywords	Lactic acid bacteria (LAB) ; Food-borne pathogens ; Green olive pickling ; Transmission electron microscopy (TEM) ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	571
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The efficacy of some probiotics and prebiotics on the prevalence of E. coli and the immune response of chickens.

Abdel-Shafi, Seham / Abd El-Hack, Mohamed E / Amen, Samy / Helmi, Anaam / Swelum, Ayman A / Tellez-Isaias, Guillermo / Enan, Gamal

Poultry science

2023 Volume 102, Issue 12, Page(s) 103219

Abstract: The present study aimed to investigate the efficacy of probiotics and prebiotics in controlling Escherichia coli (E. coli) spp. isolated from chicken. A total of 230 birds representing 19 different commercial breeds were taken from various points. Birds ... ...

Abstract	The present study aimed to investigate the efficacy of probiotics and prebiotics in controlling Escherichia coli (E. coli) spp. isolated from chicken. A total of 230 birds representing 19 different commercial breeds were taken from various points. Birds were monitored for postmortem and clinical investigation. Aseptically collected liver samples, lungs, kidneys, hearts, and yolk sacs were then subjected to bacterial isolation and identification. E. coli were observed in 9 pooled samples from 120 examined with an incidence of 7.5%. Nine farms were E. coli-positive, with an incidence of farm infection of 47.3%. The 9 suspected isolates of E. coli were profiled by morphological and microbiological identification of the colony, motility, and gram reaction. The serogroup analysis showed 9 different E. coli for which 3 other groups were identified: 2 E. coli O78, 3 E. coli O111, and 4 untyped groups. Nine isolates of E. coli were subjected to PCR. Molecular detection of 9 strains was conducted to find the virulence genes of E. coli strains (8 STX1, 4 STX2, and 9 EAE). Probiotics and prebiotics significantly increased the total erythrocytic and leukocytic counts throughout the experiment. The phagocytic percentage's main values at 14 d were 47 and 30%, respectively. An increase in the humoral immunity against Newcastle disease (ND) was noticed after ND vaccination. The geometric mean (HI) was 5.9 and 4.2 for probiotic and prebiotic, respectively. It could be concluded that probiotics and prebiotics could stimulate a nonspecific immune response against experimental infection with a virulent strain of E. coli spp.
MeSH term(s)	Animals ; Escherichia coli/physiology ; Prebiotics ; Chickens/physiology ; Prevalence ; Probiotics/pharmacology ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/prevention & control ; Escherichia coli Infections/veterinary ; Immunity, Innate
Chemical Substances	Prebiotics
Language	English
Publishing date	2023-10-20
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2023.103219
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Article ; Online: Gasdermin D

Randa El-Gamal / Mona Abdelrahim / Mohamed El-Sherbiny / Eman T. Enan / Mohammad El-Nablaway

Frontiers in Molecular Biosciences, Vol

A potential mediator and prognostic marker of bladder cancer

2022 Volume 9

Abstract: Background: Bladder cancer is considered one of the commonest widespread cancers, its presentation ranges from non-muscle invasive form to being muscle-invasive. The gasdermin family of proteins consists of six proteins. Members of gasdermin family are ... ...

Abstract	Background: Bladder cancer is considered one of the commonest widespread cancers, its presentation ranges from non-muscle invasive form to being muscle-invasive. The gasdermin family of proteins consists of six proteins. Members of gasdermin family are involved in pyroptosis; which is considered as type of inflammatory apoptosis via participation of gasdermin D and inflammatory caspases.Purpose: The goal of this research was to look into the potential involvement of gasdermin D in pathogenesis of bladder cancer, In addition, to investigate its potential role as a prognostic marker of bladder cancer.Methods: Gasdermin D gene and protein expression was examined in fresh frozen 80 bladder cancer specimens (30 NMIBC, and 50 MIBC) and the matching 80 control tissue samples utilizing real-time polymerase chain reaction and western blotting. Furthermore, the immunoreactivity of gasdermin D protein was also detected by immunohistochemistry.Results: Gasdermin D gene and protein expression showed a highly significant difference between the control and the two bladder cancer groups (p < 0.001), as demonstrated by real-time PCR, western blotting and immunohistochemistry. Cox proportional hazards regression models showed that lower gasdermin D gene expression in cancer patients (≤1.58-fold), and younger age (≤53 years) were linked with a higher risk of local tumor recurrence. Moreover, higher gasdermin D gene expression (>2.18-fold), and lymph nodes’ involvement were associated with an increased mortality.Conclusion: Gasdermin D is involved in the pathogenesis of bladder cancer and muscle invasion, in addition, tissue gasdermin D expression may be used as useful tool to predict local tumor recurrence.
Keywords	bladder cancer ; gasdermin D ; expression ; prognostic marker ; tumor recurrence ; Biology (General) ; QH301-705.5
Subject code	616 ; 610
Language	English
Publishing date	2022-09-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
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