LIVIVO - Search results -

Search results

Result 1 - 10 of total 50

Search options

Article ; Online: Ten recommendations for organising bioimaging data for archival.

Korir, Paul K / Iudin, Andrii / Somasundharam, Sriram / Weyand, Simone / Salih, Osman / Hartley, Matthew / Sarkans, Ugis / Patwardhan, Ardan / Kleywegt, Gerard J

F1000Research

2024 Volume 12

Abstract: Organised data is easy to use but the rapid developments in the field of bioimaging, with improvements in instrumentation, detectors, software and experimental techniques, have resulted in an explosion of the volumes of data being generated, making well- ... ...

Abstract	Organised data is easy to use but the rapid developments in the field of bioimaging, with improvements in instrumentation, detectors, software and experimental techniques, have resulted in an explosion of the volumes of data being generated, making well-organised data an elusive goal. This guide offers a handful of recommendations for bioimage depositors, analysts and microscope and software developers, whose implementation would contribute towards better organised data in preparation for archival. Based on our experience archiving large image datasets in EMPIAR, the BioImage Archive and BioStudies, we propose a number of strategies that we believe would improve the usability (clarity, orderliness, learnability, navigability, self-documentation, coherence and consistency of identifiers, accessibility, succinctness) of future data depositions more useful to the bioimaging community (data authors and analysts, researchers, clinicians, funders, collaborators, industry partners, hardware/software producers, journals, archive developers as well as interested but non-specialist users of bioimaging data). The recommendations that may also find use in other data-intensive disciplines. To facilitate the process of analysing data organisation, we present bandbox, a Python package that provides users with an assessment of their data by flagging potential issues, such as redundant directories or invalid characters in file or folder names, that should be addressed before archival. We offer these recommendations as a starting point and hope to engender more substantial conversations across and between the various data-rich communities.
MeSH term(s)	Humans ; Communication ; Industry ; Research Design ; Research Personnel ; Software
Language	English
Publishing date	2024-02-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2699932-8
ISSN	2046-1402 ; 2046-1402
ISSN (online)	2046-1402
ISSN	2046-1402
DOI	10.12688/f1000research.129720.2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Evaluation of the profile of interventions performed by clinical pharmacist and multidisciplinary after the implementation of the FASTHUG methodology in an intensive care unit

Alex Junior SOUZA / Fernanda Weyand BANHUK / Andressa ALMEIDA / Simone Von GROLL / Andreia Cristina SANCHES / Luciane Fátima CALDEIRA

Revista Brasileira de Farmácia Hospitalar e Serviços de Saúde, Vol 14, Iss 2, Pp 952-

2023 Volume 952

Abstract: Objective: To evaluate the profile of pharmaceutical and multidisciplinary interventions after the implementation of the FASTHUG mnemonic in a multidisciplinary meeting of an adult intensive care unit and the presence of a clinical pharmacist in this ... ...

Abstract	Objective: To evaluate the profile of pharmaceutical and multidisciplinary interventions after the implementation of the FASTHUG mnemonic in a multidisciplinary meeting of an adult intensive care unit and the presence of a clinical pharmacist in this team. Methods: Cross-sectional study, in which 225 patients were monitored, through the analysis of demographic and clinical data from electronic medical records provided by the clinical pharmacy service of the University Hospital of Western Paraná and evaluation of interventions carried out through monitoring indicators. Multidisciplinary and pharmaceutical interventions were recorded during multidisciplinary meetings and classified according to the mnemonic FASTHUG, which covers aspects such as nutrition, analgesia, sedation, thromboembolism prophylaxis, elevated headboard, stress ulcer prophylaxis and glycemic control. Results: 1078 interventions were carried out, with an average of 4.79 interventions per patient monitored. The most frequent multidisciplinary interventions were addition of necessary medication 248 (27.2%), diet optimization 145 (15.9%), analgesia optimization 128 (14%), glycemic control optimization 109 (12%), frequency of administration and suspension of unnecessary medication, both 68 (7.5%). The most common pharmaceutical interventions were optimization of administration frequency 40 (24.3%), addition of necessary medication 18 (11%), suspension of unnecessary medication 17 (10.3%) and change in dose reduction 15 (9.1%). Regarding patient outcomes, 75.1% were discharged to the ward, 24.4% died and 0.4% were transferred to another hospital. Conclusion: The implementation of the FASTHUG mnemonic in an intensive care unit provided a standardization of language among the multidisciplinary team during clinical meetings, as well as enabling the evaluation of essential points of pharmacotherapy by the clinical pharmacist.
Keywords	Public aspects of medicine ; RA1-1270 ; Pharmacy and materia medica ; RS1-441 ; Therapeutics. Pharmacology ; RM1-950
Subject code	610
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Sociedade Brasileira de Farmácia Hospitalar e Serviços de Saúde
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Advances in membrane protein crystallography: in situ and in meso data collection.

Weyand, Simone / Tate, Christopher G

Acta crystallographica. Section D, Biological crystallography

2015 Volume 71, Issue Pt 6, Page(s) 1226–1227

MeSH term(s)	Crystallography, X-Ray/methods ; Membrane Proteins/chemistry
Chemical Substances	Membrane Proteins
Language	English
Publishing date	2015-05-23
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	2020492-9
ISSN	1399-0047 ; 0907-4449
ISSN (online)	1399-0047
ISSN	0907-4449
DOI	10.1107/S1399004715008317
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: EMPIAR: the Electron Microscopy Public Image Archive.

Iudin, Andrii / Korir, Paul K / Somasundharam, Sriram / Weyand, Simone / Cattavitello, Cesare / Fonseca, Neli / Salih, Osman / Kleywegt, Gerard J / Patwardhan, Ardan

Nucleic acids research

2022 Volume 51, Issue D1, Page(s) D1503–D1511

Abstract: Public archiving in structural biology is well established with the Protein Data Bank (PDB; wwPDB.org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; emdb-empiar.org) for 3D reconstructions from cryo-EM experiments. Even before ... ...

Abstract	Public archiving in structural biology is well established with the Protein Data Bank (PDB; wwPDB.org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; emdb-empiar.org) for 3D reconstructions from cryo-EM experiments. Even before the recent rapid growth in cryo-EM, there was an expressed community need for a public archive of image data from cryo-EM experiments for validation, software development, testing and training. Concomitantly, the proliferation of 3D imaging techniques for cells, tissues and organisms using volume EM (vEM) and X-ray tomography (XT) led to calls from these communities to publicly archive such data as well. EMPIAR (empiar.org) was developed as a public archive for raw cryo-EM image data and for 3D reconstructions from vEM and XT experiments and now comprises over a thousand entries totalling over 2 petabytes of data. EMPIAR resources include a deposition system, entry pages, facilities to search, visualize and download datasets, and a REST API for programmatic access to entry metadata. The success of EMPIAR also poses significant challenges for the future in dealing with the very fast growth in the volume of data and in enhancing its reusability.
MeSH term(s)	Imaging, Three-Dimensional ; Microscopy, Electron ; Software ; Databases, Factual
Language	English
Publishing date	2022-11-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gkac1062
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1067: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: kcnj13 regulates pigment cell shapes in zebrafish and has diverged by cis-regulatory evolution between Danio species.

Podobnik, Marco / Singh, Ajeet P / Fu, Zhenqiang / Dooley, Christopher M / Frohnhöfer, Hans Georg / Firlej, Magdalena / Stednitz, Sarah J / Elhabashy, Hadeer / Weyand, Simone / Weir, John R / Lu, Jianguo / Nüsslein-Volhard, Christiane / Irion, Uwe

Development (Cambridge, England)

2023 Volume 150, Issue 16

Abstract: Teleost fish of the genus Danio are excellent models to study the genetic and cellular bases of pigment pattern variation in vertebrates. The two sister species Danio rerio and Danio aesculapii show divergent patterns of horizontal stripes and vertical ... ...

Abstract	Teleost fish of the genus Danio are excellent models to study the genetic and cellular bases of pigment pattern variation in vertebrates. The two sister species Danio rerio and Danio aesculapii show divergent patterns of horizontal stripes and vertical bars that are partly caused by the divergence of the potassium channel gene kcnj13. Here, we show that kcnj13 is required only in melanophores for interactions with xanthophores and iridophores, which cause location-specific pigment cell shapes and thereby influence colour pattern and contrast in D. rerio. Cis-regulatory rather than protein coding changes underlie kcnj13 divergence between the two Danio species. Our results suggest that homotypic and heterotypic interactions between the pigment cells and their shapes diverged between species by quantitative changes in kcnj13 expression during pigment pattern diversification.
MeSH term(s)	Animals ; Cell Shape ; Melanophores/physiology ; Pigmentation/genetics ; Skin ; Zebrafish/genetics
Chemical Substances	Kir7.1 channel
Language	English
Publishing date	2023-08-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	90607-4
ISSN	1477-9129 ; 0950-1991
ISSN (online)	1477-9129
ISSN	0950-1991
DOI	10.1242/dev.201627
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ub I Zs.183: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 91: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Molecular recognition of an aversive odorant by the murine trace amine-associated receptor TAAR7f.

Gusach, Anastasiia / Lee, Yang / Khoshgrudi, Armin Nikpour / Mukhaleva, Elizaveta / Ma, Ning / Koers, Eline J / Chen, Qingchao / Edwards, Patricia C / Huang, Fanglu / Kim, Jonathan / Mancia, Filippo / Verprintsev, Dmitry B / Vaidehi, Nagarajan / Weyand, Simone N / Tate, Christopher G

bioRxiv : the preprint server for biology

2023

Abstract: There are two main families of G protein-coupled receptors that detect odours in humans, the odorant receptors (ORs) and the trace amine-associated receptors (TAARs). Their amino acid sequences are distinct, with the TAARs being most similar to the ... ...

Abstract	There are two main families of G protein-coupled receptors that detect odours in humans, the odorant receptors (ORs) and the trace amine-associated receptors (TAARs). Their amino acid sequences are distinct, with the TAARs being most similar to the aminergic receptors such as those activated by adrenaline, serotonin and histamine. To elucidate the structural determinants of ligand recognition by TAARs, we have determined the cryo-EM structure of a murine receptor, mTAAR7f, coupled to the heterotrimeric G protein G
Language	English
Publishing date	2023-07-07
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.07.07.547762
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Focal Pulsed Field Ablation for Atrial Arrhythmias: Efficacy and Safety under Deep Sedation.

Weyand, Sebastian / Adam, Viola / Biehler, Paloma / Hägele, Patricia / Hanger, Simon / Heinzmann, David / Löbig, Stephanie / Pinchuk, Andrei / Waechter, Christian / Seizer, Peter

Journal of clinical medicine

2024 Volume 13, Issue 2

Abstract: Focal pulsed field ablation (PFA) is a novel technique for treating cardiac arrhythmias. It has demonstrated positive results in initial studies and has a good safety profile. In recent studies, PFA was often utilized for first-time pulmonary vein ... ...

Abstract	Focal pulsed field ablation (PFA) is a novel technique for treating cardiac arrhythmias. It has demonstrated positive results in initial studies and has a good safety profile. In recent studies, PFA was often utilized for first-time pulmonary vein isolation (PVI) and was performed under general anesthesia. In our study, we assessed the feasibility, safety, acute procedural efficacy, and efficiency of focal PFA under deep sedation in patients, 80% of whom had undergone at least one left atrial ablation previously. We treated 30 patients (71 ± 7, 46% male) using the CENTAURI system for various atrial arrhythmias, including atrial fibrillation, typical and atypical atrial flutter, and focal atrial tachycardia. The average procedure and fluoroscopy times were 122 ± 43 min and 9 ± 7 min, respectively. A total of 83.33% of patients received additional line ablations beyond PVI, specifically targeting the posterior box and anterior mitral line. All ablations were successfully performed in deep sedation with only one major and one minor complication observed. The major complication was a vasospasm of the right coronary artery during ablation of the cavotricuspid isthmus, which was treated successfully with intracoronary nitroglycerin. All patients could be discharged in sinus rhythm. Moreover, adenosine appears effective in identifying dormant conduction in some patients after focal PFA. In conclusion, focal PFA is an effective approach for complex left atrial ablations under deep sedation, offering both high efficacy and efficiency with a reliable safety profile. Studies on long-term outcomes are needed.
Language	English
Publishing date	2024-01-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm13020576
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Effect of Left Atrial Pulmonary Vein Angiography on Safety and Efficacy for High-Power, Short-Duration Pulmonary Vein Isolation in Patients with Atrial Fibrillation.

Weyand, Sebastian / Adam, Viola / Beuter, Matthias / Hanger, Simon / Heinzmann, David / Schrezenmeier, Willibald / Seizer, Peter

Journal of clinical medicine

2023 Volume 12, Issue 3

Abstract: Imaging of pulmonary vein (PV) anatomy by angiography before pulmonary vein isolation (PVI) for atrial fibrillation (AF) has long been standard practice in many centers. Nowadays, very accurate anatomical maps can be generated by the use of high- ... ...

Abstract	Imaging of pulmonary vein (PV) anatomy by angiography before pulmonary vein isolation (PVI) for atrial fibrillation (AF) has long been standard practice in many centers. Nowadays, very accurate anatomical maps can be generated by the use of high-resolution mapping catheters, and very effective ablation lesions can be generated by the use of the high-power, short-duration (HPSD) technique. In our center, PV angiography was routinely performed before PVI. However, since there is no clear evidence for this, we refrained from performing PV angiography. This study aimed to investigate whether PV angiography is still necessary when using high-resolution mapping catheters after ablation in the high-power, short-duration (HPSD) technique. A total of 139 consecutive patients with atrial fibrillation (66.25 ± 11.68 years old, 62.39% male) undergoing radiofrequency PVI were included in the study. Ablation was performed with the HPSD technique using a fixed protocol for energy delivery of 50 watts (contact force 3-20 g). We observed no significant effect on the efficacy, efficiency and complications of the ablation procedure if pulmonary vein angiography was omitted before HPSD PVI. Thus, using our protocol, it may be useful that PV angiography is avoided, especially in young patients and those with chronic renal disease.
Language	English
Publishing date	2023-01-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm12031094
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Biochemistry. Old gate gets a new look.

Weyand, Simone / Iwata, So

Science (New York, N.Y.)

2010 Volume 329, Issue 5988, Page(s) 151–152

MeSH term(s)	Animals ; Binding Sites ; Calcium/metabolism ; Humans ; Ion Channel Gating ; Large-Conductance Calcium-Activated Potassium Channels/chemistry ; Large-Conductance Calcium-Activated Potassium Channels/metabolism ; Models, Molecular ; Protein Conformation ; Protein Structure, Tertiary
Chemical Substances	Large-Conductance Calcium-Activated Potassium Channels ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2010-07-09
Publishing country	United States
Document type	Comment ; Journal Article
ZDB-ID	128410-1
ISSN	1095-9203 ; 0036-8075
ISSN (online)	1095-9203
ISSN	0036-8075
DOI	10.1126/science.1192680
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 27: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 77: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A Light-Oxygen-Voltage Receptor Integrates Light and Temperature.

Dietler, Julia / Schubert, Roman / Krafft, Tobias G A / Meiler, Simone / Kainrath, Stephanie / Richter, Florian / Schweimer, Kristian / Weyand, Michael / Janovjak, Harald / Möglich, Andreas

Journal of molecular biology

2021 Volume 433, Issue 15, Page(s) 167107

Abstract: Sensory photoreceptors enable organisms to adjust their physiology, behavior, and development in response to light, generally with spatiotemporal acuity and reversibility. These traits underlie the use of photoreceptors as genetically encoded actuators ... ...

Abstract	Sensory photoreceptors enable organisms to adjust their physiology, behavior, and development in response to light, generally with spatiotemporal acuity and reversibility. These traits underlie the use of photoreceptors as genetically encoded actuators to alter by light the state and properties of heterologous organisms. Subsumed as optogenetics, pertinent approaches enable regulating diverse cellular processes, not least gene expression. Here, we controlled the widely used Tet repressor by coupling to light-oxygen-voltage (LOV) modules that either homodimerize or dissociate under blue light. Repression could thus be elevated or relieved, and consequently protein expression was modulated by light. Strikingly, the homodimeric RsLOV module from Rhodobacter sphaeroides not only dissociated under light but intrinsically reacted to temperature. The limited light responses of wild-type RsLOV at 37 °C were enhanced in two variants that exhibited closely similar photochemistry and structure. One variant improved the weak homodimerization affinity of 40 µM by two-fold and thus also bestowed light sensitivity on a receptor tyrosine kinase. Certain photoreceptors, exemplified by RsLOV, can evidently moonlight as temperature sensors which immediately bears on their application in optogenetics and biotechnology. Properly accounted for, the temperature sensitivity can be leveraged for the construction of signal-responsive cellular circuits.
MeSH term(s)	Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Gene Expression Regulation, Bacterial ; Models, Molecular ; Optogenetics ; Oxygen/metabolism ; Protein Domains ; Protein Stability ; Protein Structure, Secondary ; Receptor Protein-Tyrosine Kinases ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Rhodobacter sphaeroides/chemistry ; Rhodobacter sphaeroides/metabolism ; Temperature
Chemical Substances	Bacterial Proteins ; Repressor Proteins ; tetracycline resistance-encoding transposon repressor protein ; Receptor Protein-Tyrosine Kinases (EC 2.7.10.1) ; Oxygen (S88TT14065)
Language	English
Publishing date	2021-06-17
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2021.167107
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 867: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito