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  1. Article ; Online: Affective psychosis after COVID-19 infection in a previously healthy patient: a case report.

    Correa-Palacio, Andres F / Hernandez-Huerta, Daniel / Gómez-Arnau, Jorge / Loeck, Carmen / Caballero, Irene

    Psychiatry research

    2020  Volume 290, Page(s) 113115

    MeSH term(s) Affective Disorders, Psychotic ; Betacoronavirus ; COVID-19 ; Coronavirus Infections ; Humans ; Infections ; Pandemics ; Pneumonia, Viral ; Psychotic Disorders ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-05-21
    Publishing country Ireland
    Document type Case Reports ; Comment
    ZDB-ID 445361-x
    ISSN 1872-7123 ; 1872-7506 ; 0925-4927 ; 0165-1781
    ISSN (online) 1872-7123 ; 1872-7506
    ISSN 0925-4927 ; 0165-1781
    DOI 10.1016/j.psychres.2020.113115
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Affective psychosis after COVID-19 infection in a previously healthy patient: a case report

    Correa-Palacio, Andres F / Hernandez-Huerta, Daniel / Gómez-Arnau, Jorge / Loeck, Carmen / Caballero, Irene

    Psychiatry Res

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #591084
    Database COVID19

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  3. Article ; Online: Affective psychosis after COVID-19 infection in a previously healthy patient

    Correa-Palacio, Andres F / Hernandez-Huerta, Daniel / Gómez-Arnau, Jorge / Loeck, Carmen / Caballero, Irene

    Psychiatry Research

    a case report

    2020  Volume 290, Page(s) 113115

    Keywords Biological Psychiatry ; Psychiatry and Mental health ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 445361-x
    ISSN 1872-7123 ; 1872-7506 ; 0925-4927 ; 0165-1781
    ISSN (online) 1872-7123 ; 1872-7506
    ISSN 0925-4927 ; 0165-1781
    DOI 10.1016/j.psychres.2020.113115
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Validación de una nueva estrategia para la identificación de variantes de SARS-CoV-2 mediante secuenciación del gen espiga por Sanger.

    Murillo, Enderson / Palacio-Rua, Katherine / Afanador-Ayala, Carlos / García-Correa, Juan Felipe / Zuluaga, Andrés F

    Enfermedades infecciosas y microbiologia clinica

    2022  Volume 41, Issue 5, Page(s) 284–289

    Abstract: Introduction: The emergence of multiple variants of SARS-CoV-2 during the COVID-19 pandemic is of great world concern. Until now, their analysis has mainly focused on next-generation sequencing. However, this technique is expensive and requires ... ...

    Title translation Validation of a new strategy for the identification of SARS-CoV-2 variants by sequencing the spike gene by Sanger.
    Abstract Introduction: The emergence of multiple variants of SARS-CoV-2 during the COVID-19 pandemic is of great world concern. Until now, their analysis has mainly focused on next-generation sequencing. However, this technique is expensive and requires sophisticated equipment, long processing times, and highly qualified technical personnel with experience in bioinformatics. To contribute to the analysis of variants of interest and variants of concern, increase the diagnostic capacity, and process samples to carry out genomic surveillance, we propose a quick and easy methodology to apply, based on Sanger sequencing of 3 gene fragments that code for protein spike.
    Methods: Fifteen positive samples for SARS-CoV-2 with a cycle threshold below 25 were sequenced by Sanger and next-generation sequencing methodologies. The data obtained were analyzed on the Nextstrain and PANGO Lineages platforms.
    Results: Both methodologies allowed the identification of the variants of interest reported by the WHO. Two samples were identified as Alpha, 3 Gamma, one Delta, 3 Mu, one Omicron, and 5 strains were close to the initial Wuhan-Hu-1 virus isolate. According to
    Conclusion: The different SARS-CoV-2 lineages of interest and concern are classified quickly, agilely, and reliably with the Sanger sequencing methodology.
    Language Spanish
    Publishing date 2022-05-16
    Publishing country Spain
    Document type English Abstract ; Journal Article
    ZDB-ID 1070941-1
    ISSN 1578-1852 ; 0213-005X
    ISSN (online) 1578-1852
    ISSN 0213-005X
    DOI 10.1016/j.eimc.2022.04.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Validation of a new strategy for the identification of SARS-CoV-2 variants by sequencing the spike gene by Sanger.

    Murillo, Enderson / Palacio-Rua, Katherine / Afanador-Ayala, Carlos / García-Correa, Juan Felipe / Zuluaga, Andrés F

    Enfermedades infecciosas y microbiologia clinica (English ed.)

    2022  Volume 41, Issue 5, Page(s) 284–289

    Abstract: Introduction: The emergence of multiple variants of SARS-CoV-2 during the COVID-19 pandemic is of great world concern. Until now, their analysis has mainly focused on next-generation sequencing. However, this technique is expensive and requires ... ...

    Abstract Introduction: The emergence of multiple variants of SARS-CoV-2 during the COVID-19 pandemic is of great world concern. Until now, their analysis has mainly focused on next-generation sequencing. However, this technique is expensive and requires sophisticated equipment, long processing times, and highly qualified technical personnel with experience in bioinformatics. To contribute to the analysis of variants of interest and variants of concern, increase the diagnostic capacity, and process samples to carry out genomic surveillance, we propose a quick and easy methodology to apply, based on Sanger sequencing of 3 gene fragments that code for protein spike.
    Methods: Fifteen positive samples for SARS-CoV-2 with a cycle threshold below 25 were sequenced by Sanger and next-generation sequencing methodologies. The data obtained were analyzed on the Nextstrain and PANGO Lineages platforms.
    Results: Both methodologies allowed the identification of the variants of interest reported by the WHO. Two samples were identified as Alpha, 3 Gamma, one Delta, 3 Mu, one Omicron, and 5 strains were close to the initial Wuhan-Hu-1 virus isolate. According to in silico analysis, key mutations can also be detected to identify and classify other variants not evaluated in the study.
    Conclusion: The different SARS-CoV-2 lineages of interest and concern are classified quickly, agilely, and reliably with the Sanger sequencing methodology.
    MeSH term(s) Humans ; SARS-CoV-2/genetics ; COVID-19/diagnosis ; Pandemics ; High-Throughput Nucleotide Sequencing
    Language English
    Publishing date 2022-10-17
    Publishing country Spain
    Document type Journal Article
    ISSN 2529-993X
    ISSN (online) 2529-993X
    DOI 10.1016/j.eimce.2022.10.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Validation of a duplex PCR technique using the gen E and RNase P for the diagnosis of SARS-CoV-2.

    Palacio Rua, Katherine / García Correa, Juan Felipe / Aguilar-Jiménez, Wbeimar / Afanador Ayala, Carlos / Rugeles, María Teresa / Zuluaga, Andrés F

    Enfermedades infecciosas y microbiologia clinica (English ed.)

    2022  Volume 40, Issue 8, Page(s) 428–435

    Abstract: Introduction: Reverse transcriptase - polymerase chain reaction (RT-PCR) is the standard technique for SARS-CoV-2 diagnosis. The World Health Organization recommends the Charité-Berlin protocol for COVID-19 diagnosis, which requires triple PCR, limiting ...

    Abstract Introduction: Reverse transcriptase - polymerase chain reaction (RT-PCR) is the standard technique for SARS-CoV-2 diagnosis. The World Health Organization recommends the Charité-Berlin protocol for COVID-19 diagnosis, which requires triple PCR, limiting the process capability of laboratories and delaying the results. In order to reduce these limitations, a duplex PCR is validated for the detection of the E and ribonuclease P genes.
    Methods: We compared the limit of detection, sensitivity and specificity of the duplex PCR technique (E gene and Rnasa P) against the monoplex standard (E gene) in RNA samples from a SARS-CoV-2 isolate and 88 clinical specimens with previously known results. The repeatability and reproducibility of the threshold cycle values ​​(Ct) were determined in two independent laboratories of the Faculty of Medicine of the Universidad de Antioquia, using different reagents and real time instruments.
    Results: There were no significant differences in the Ct results between both techniques (P = .84). Using the monoplex PCR of E gene as a reference, the interrater reliability analysis showed similarity between the two techniques, with a kappa coefficient of 0.89, the sensitivity and the specificity of duplex PCR were 90% and 87%, respectively.
    Conclusions: Duplex PCR does not affect the sensitivity and specificity reported by the Charité, Berlin protocol, being a useful tool for SARS-CoV-2 screening in clinical samples.
    MeSH term(s) COVID-19/diagnosis ; COVID-19 Testing ; Humans ; Polymerase Chain Reaction ; RNA, Viral/analysis ; RNA-Directed DNA Polymerase/genetics ; Reproducibility of Results ; Ribonuclease P/genetics ; SARS-CoV-2/genetics
    Chemical Substances RNA, Viral ; RNA-Directed DNA Polymerase (EC 2.7.7.49) ; Ribonuclease P (EC 3.1.26.5)
    Language English
    Publishing date 2022-05-19
    Publishing country Spain
    Document type Journal Article
    ISSN 2529-993X
    ISSN (online) 2529-993X
    DOI 10.1016/j.eimce.2022.05.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Validation of a duplex PCR technique using the gen E and RNase P for the diagnosis of SARS-CoV-2.

    Palacio Rua, Katherine / García Correa, Juan Felipe / Aguilar-Jiménez, Wbeimar / Afanador Ayala, Carlos / Rugeles, María Teresa / Zuluaga, Andrés F

    Enfermedades infecciosas y microbiologia clinica (English ed.)

    2021  

    Abstract: Introduction: Reverse transcriptase - polymerase chain reaction (RT-PCR) is the standard technique for SARS-CoV-2 diagnosis. The World Health Organization recommends the Charité-Berlin protocol for COVID-19 diagnosis, which requires triple PCR, limiting ...

    Title translation Validación de una técnica de PCR dúplex usando el gen E y RNasa P para el diagnóstico de SARS-CoV-2.
    Abstract Introduction: Reverse transcriptase - polymerase chain reaction (RT-PCR) is the standard technique for SARS-CoV-2 diagnosis. The World Health Organization recommends the Charité-Berlin protocol for COVID-19 diagnosis, which requires triple PCR, limiting the process capability of laboratories and delaying the results. In order to reduce these limitations, a duplex PCR is validated for the detection of the E and RNase P genes.
    Methods: We compared the limit of detection, sensitivity and specificity of the duplex PCR technique (E gene and RNase P) against the monoplex standard (E gene) in RNA samples from a SARS-CoV-2 isolate and 88 clinical specimens with previously known results. The repeatability and reproducibility of the threshold cycle values (Ct) were determined in two independent laboratories of the Faculty of Medicine of the Universidad de Antioquia, using different reagents and real time instruments.
    Results: There were no significant differences in the Ct results between both techniques (p = 0.84). Using the monoplex PCR of E gene as a reference, the interrater reliability analysis showed similarity between the two techniques, with a kappa coefficient of 0.89, the sensitivity and the specificity of duplex PCR were 90% and 87%, respectively.
    Conclusions: Duplex PCR does not affect the sensitivity and specificity reported by the Charité, Berlin protocol, being a useful tool for SARS-CoV-2 screening in clinical samples.
    Language Spanish
    Publishing date 2021-01-19
    Publishing country Spain
    Document type Journal Article
    ISSN 2529-993X
    ISSN (online) 2529-993X
    DOI 10.1016/j.eimc.2020.12.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Photocatalytic performance evaluation of flame‐sprayed and polymeric suspension coatings from TiO2 nanoparticles

    Palacio, Claudia / Alvarez, Alejandro / Mejía‐Roldán, Andrés F / Urquijo, Jeaneth P / Arias, Jhoman / Vélez‐Monsalve, Leidy C / Mejía‐Sepúlveda, Juliana / Saldarriaga, Julio C / Correa, Mauricio A / Vargas, Fabio

    Journal of Chemical Technology & Biotechnology. 2023 Aug., v. 98, no. 8 p.1819-1825

    2023  

    Abstract: BACKGROUND: Heterogeneous photocatalysis offers an interesting alternative to mitigate air pollution by using materials capable of reacting with toxic gases under ultraviolet light. One of these materials is titanium dioxide (TiO₂), and thus it is ... ...

    Abstract BACKGROUND: Heterogeneous photocatalysis offers an interesting alternative to mitigate air pollution by using materials capable of reacting with toxic gases under ultraviolet light. One of these materials is titanium dioxide (TiO₂), and thus it is necessary to analyze and improve its photocatalytic response for air depollution, in order to facilitate its use in surface applications. RESULTS: The photocatalytic ability of the synthesized TiO₂ particles to remove nitric oxide (NO) was tested, and it was found that both the anatase and rutile phases present in the samples led to a NO abatement capacity of ≈60% η NO. Two different methods to coat surfaces with TiO₂ particles were evaluated: thermal spray by oxyacetylene flame and dripping of polymeric suspensions of TiO₂. A more detrimental effect on photocatalytic efficiencies was found using polymeric suspension rather than flame‐spraying as coating method. This was despite the anatase transformation to rutile and Ti₇O₁₃ that TiO₂ particles undergo using the latter process as a consequence of heat transfer from the flame to the particles during the coating formation. CONCLUSION: Results obtained in this research demonstrate that polymeric suspensions inhibit the photocatalytic activity of TiO₂ nanoparticles, probably due to the formation of a polymeric matrix on samples that constrains the action of pollutant and testing factors on samples. However, the thermal method can help retain part of the photocatalytic capability to abate NOx, despite depletion in the anatase content resulting from the heat transfer to the TiO₂ raw particles sprayed to form coatings. © 2023 Society of Chemical Industry (SCI).
    Keywords air ; air pollution ; biotechnology ; heat transfer ; nanoparticles ; nitric oxide ; photocatalysis ; pollutants ; polymers ; titanium dioxide ; toxicity ; ultraviolet radiation
    Language English
    Dates of publication 2023-08
    Size p. 1819-1825.
    Publishing place John Wiley & Sons, Ltd.
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 1479465-2
    ISSN 1097-4660 ; 0268-2575
    ISSN (online) 1097-4660
    ISSN 0268-2575
    DOI 10.1002/jctb.7323
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: First large-scale study reveals important losses of managed honey bee and stingless bee colonies in Latin America.

    Requier, Fabrice / Leyton, Malena Sibaja / Morales, Carolina L / Garibaldi, Lucas A / Giacobino, Agostina / Porrini, Martin Pablo / Rosso-Londoño, Juan Manuel / Velarde, Rodrigo A / Aignasse, Andrea / Aldea-Sánchez, Patricia / Allasino, Mariana Laura / Arredondo, Daniela / Audisio, Carina / Cagnolo, Natalia Bulacio / Basualdo, Marina / Branchiccela, Belén / Calderón, Rafael A / Castelli, Loreley / Castilhos, Dayson /
    Escareño, Francisca Contreras / Correa-Benítez, Adriana / da Silva, Fabiana Oliveira / Garnica, Diego Silva / de Groot, Grecia / Delgado-Cañedo, Andres / Fernández-Marín, Hermógenes / Freitas, Breno M / Galindo-Cardona, Alberto / Garcia, Nancy / Garrido, Paula M / Giray, Tugrul / Gonçalves, Lionel Segui / Landi, Lucas / Malusá Gonçalves, Daniel / Martinez, Silvia Inés / Moja, Pablo Joaquín / Molineri, Ana / Müller, Pablo Fernando / Nogueira, Enrique / Pacini, Adriana / Palacio, María Alejandra / Parra, Guiomar Nates / Parra-H, Alejandro / Peres Gramacho, Kátia / Castro, Eleazar Pérez / Pires, Carmen Sílvia Soares / Reynaldi, Francisco J / Luis, Anais Rodríguez / Rossini, Carmen / Sánchez Armijos, Milton / Santos, Estela / Scannapieco, Alejandra / Spina, Yamandú Mendoza / Tapia González, José María / Vargas Fernández, Andrés Marcelo / Viana, Blandina Felipe / Vieli, Lorena / Yadró García, Carlos Ariel / Antúnez, Karina

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 10079

    Abstract: Over the last quarter century, increasing honey bee colony losses motivated standardized large-scale surveys of managed honey bees (Apis mellifera), particularly in Europe and the United States. Here we present the first large-scale standardized survey ... ...

    Abstract Over the last quarter century, increasing honey bee colony losses motivated standardized large-scale surveys of managed honey bees (Apis mellifera), particularly in Europe and the United States. Here we present the first large-scale standardized survey of colony losses of managed honey bees and stingless bees across Latin America. Overall, 1736 beekeepers and 165 meliponiculturists participated in the 2-year survey (2016-2017 and 2017-2018). On average, 30.4% of honey bee colonies and 39.6% of stingless bee colonies were lost per year across the region. Summer losses were higher than winter losses in stingless bees (30.9% and 22.2%, respectively) but not in honey bees (18.8% and 20.6%, respectively). Colony loss increased with operation size during the summer in both honey bees and stingless bees and decreased with operation size during the winter in stingless bees. Furthermore, losses differed significantly between countries and across years for both beekeepers and meliponiculturists. Overall, winter losses of honey bee colonies in Latin America (20.6%) position this region between Europe (12.5%) and the United States (40.4%). These results highlight the magnitude of bee colony losses occurring in the region and suggest difficulties in maintaining overall colony health and economic survival for beekeepers and meliponiculturists.
    MeSH term(s) Animals ; Bees/physiology ; Latin America ; Beekeeping ; Seasons
    Language English
    Publishing date 2024-05-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-59513-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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