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Article ; Online: Reducing the stigma of academic mental health can save lives.

Schueth, Anna

2021 Volume 19, Issue 3, Page(s) 129–130

MeSH term(s)	Humans ; Mental Disorders/therapy ; Mental Health ; Social Stigma
Language	English
Publishing date	2021-12-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2493737-X
ISSN	1759-4820 ; 1759-4812
ISSN (online)	1759-4820
ISSN	1759-4812
DOI	10.1038/s41585-021-00549-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Den kritischen Zeitpunkt nicht verpassen

Edenhofer, Ottmar / Grimm, Veronika / Haug, Gerald H. / Marotzke, Jochem / Marquardt, Wolfgang / Schlögl, Robert / Schmidt, Christoph M. / Schüth, Ferdi / Wagner, Ulrich / Anton, Christian / Artmann, Stefan / Happe, Kathrin / Schmoldt, Johannes / Winkler, Matthias / Wetterich, Sebastian

Leitideen für die Transformation des Energiesystems

(Diskussion / Deutsche Akademie der Naturforscher Leopoldina ; Nr. 31 (2023))

2023

Abstract: Um in Deutschland und Europa die Pariser Klimaziele zu erreichen, muss das Energiesystem transformiert werden. Wichtig ist zudem die Bereitstellung von Technologien, die dies auch weltweit ermöglichen. Der Handlungsbedarf ist groß, weil vielfältige ... ...

Institution	Deutsche Akademie der Naturforscher Leopoldina
Author's details	Ottmar Edenhofer, Veronika Grimm, Gerald Haug, Jochem Marotzke, Wolfgang Marquardt, Robert Schlögl, Christoph M. Schmidt, Ferdi Schüth, Ulrich Wagner ; Herausgeber: Deutsche Akademie der Naturforscher Leopoldina e.V. - Nationale Akademie der Wissenschaften ; Redaktion: Christian Anton, Stefan Artmann, Kathrin Happe, Johannes Schmoldt, Matthias Winkler, Sebastian Wetterich (Nationale Akademie der Wissenschaften Leopoldina, Abteilung Wissenschaft - Politik - Gesellschaft)
Series title	Diskussion / Deutsche Akademie der Naturforscher Leopoldina ; Nr. 31 (2023) Diskussion / Deutsche Akademie der Naturforscher Leopoldina - Nationale Akademie der Wissenschaften Diskussion
Collection	Diskussion / Deutsche Akademie der Naturforscher Leopoldina - Nationale Akademie der Wissenschaften Diskussion
Abstract	Um in Deutschland und Europa die Pariser Klimaziele zu erreichen, muss das Energiesystem transformiert werden. Wichtig ist zudem die Bereitstellung von Technologien, die dies auch weltweit ermöglichen. Der Handlungsbedarf ist groß, weil vielfältige internationale Krisen wie der russische Angriffskrieg auf die Ukraine Zielkonflikte verschärfen und die globale Kooperation erschweren, die notwendig ist, um das Ziel der Klimaneutralität zu erreichen. Das Leopoldina-Diskussionspapier „Leitideen für die Transformation des Energiesystems“ erörtert hierzu Ansätze für eine effektive und tragfähige Energiewende.
Keywords	Energiewende ; Klimaänderung
Subject	Klima ; Klimawandel ; Klimaveränderung ; Klimawechsel ; Energietransition ; Energiesystemtransformation
Language	German
Size	1 Online-Ressource (18 Seiten, 0,38 MB)
Publisher	Deutsche Akademie der Naturforscher Leopoldina e.V. - Nationale Akademie der Wissenschaften
Publishing place	Halle (Saale)
Publishing country	Germany
Document type	Book ; Online
Note	Literaturverzeichnis: Seite 15-16 ; Redaktionsschluss 03.03.2023
HBZ-ID	HT030619259
DOI	10.26164/leopoldina_03_00782
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Efficient 3D light-sheet imaging of very large-scale optically cleared human brain and prostate tissue samples.

Schueth, Anna / Hildebrand, Sven / Samarska, Iryna / Sengupta, Shubharthi / Kiessling, Annemarie / Herrler, Andreas / Zur Hausen, Axel / Capalbo, Michael / Roebroeck, Alard

Communications biology

2023 Volume 6, Issue 1, Page(s) 170

Abstract: The ability to image human tissue samples in 3D, with both cellular resolution and a large field of view (FOV), can improve fundamental and clinical investigations. Here, we demonstrate the feasibility of light-sheet imaging of ~5 ... ...

Abstract	The ability to image human tissue samples in 3D, with both cellular resolution and a large field of view (FOV), can improve fundamental and clinical investigations. Here, we demonstrate the feasibility of light-sheet imaging of ~5 cm
MeSH term(s)	Male ; Humans ; Prostate/diagnostic imaging ; Microscopy/methods ; Brain/diagnostic imaging ; Prostatic Neoplasms/diagnostic imaging ; Formaldehyde
Chemical Substances	Formaldehyde (1HG84L3525)
Language	English
Publishing date	2023-02-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-04536-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online ; Thesis: Synthesis of oxymethylene ethers

Grünert, Anna [Verfasser] / Schüth, Ferdi [Gutachter] / Muhler, Martin [Gutachter]

2020

Author's details	Anna Grünert ; Gutachter: Ferdi Schüth, Martin Muhler ; Fakultät für Chemie und Biochemie
Keywords	Technische Chemie ; Technical Chemistry
Subject code	sg660
Language	English
Publisher	Ruhr-Universität Bochum
Publishing place	Bochum
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Article ; Online: Scalable Labeling for Cytoarchitectonic Characterization of Large Optically Cleared Human Neocortex Samples.

Hildebrand, Sven / Schueth, Anna / Herrler, Andreas / Galuske, Ralf / Roebroeck, Alard

Scientific reports

2019 Volume 9, Issue 1, Page(s) 10880

Abstract: Optical clearing techniques and light sheet microscopy have transformed fluorescent imaging of rodent brains, and have provided a crucial alternative to traditional confocal or bright field techniques for thin sections. However, clearing and labeling ... ...

Abstract	Optical clearing techniques and light sheet microscopy have transformed fluorescent imaging of rodent brains, and have provided a crucial alternative to traditional confocal or bright field techniques for thin sections. However, clearing and labeling human brain tissue through all cortical layers and significant portions of a cortical area, has so far remained extremely challenging, especially for formalin fixed adult cortical tissue. Here, we present MASH (Multiscale Architectonic Staining of Human cortex): a simple, fast and low-cost cytoarchitectonic labeling approach for optically cleared human cortex samples, which can be applied to large (up to 5 mm thick) formalin fixed adult brain samples. A suite of small-molecule fluorescent nuclear and cytoplasmic dye protocols in combination with new refractive index matching solutions allows deep volume imaging. This greatly reduces time and cost of imaging cytoarchitecture in thick samples and enables classification of cytoarchitectonic layers over the full cortical depth. We demonstrate application of MASH to large archival samples of human visual areas, characterizing cortical architecture in 3D from the scale of cortical areas to that of single cells. In combination with scalable light sheet imaging and data analysis, MASH could open the door to investigation of large human cortical systems at cellular resolution and in the context of their complex 3-dimensional geometry.
MeSH term(s)	Adult ; Humans ; Imaging, Three-Dimensional ; Microscopy, Confocal ; Microtomy ; Neocortex/anatomy & histology ; Neocortex/cytology ; Optics and Photonics/methods ; Staining and Labeling/methods
Language	English
Publishing date	2019-07-26
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-47336-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Scalable Labeling for Cytoarchitectonic Characterization of Large Optically Cleared Human Neocortex Samples

Sven Hildebrand / Anna Schueth / Andreas Herrler / Ralf Galuske / Alard Roebroeck

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 10

Abstract: Abstract Optical clearing techniques and light sheet microscopy have transformed fluorescent imaging of rodent brains, and have provided a crucial alternative to traditional confocal or bright field techniques for thin sections. However, clearing and ... ...

Abstract	Abstract Optical clearing techniques and light sheet microscopy have transformed fluorescent imaging of rodent brains, and have provided a crucial alternative to traditional confocal or bright field techniques for thin sections. However, clearing and labeling human brain tissue through all cortical layers and significant portions of a cortical area, has so far remained extremely challenging, especially for formalin fixed adult cortical tissue. Here, we present MASH (Multiscale Architectonic Staining of Human cortex): a simple, fast and low-cost cytoarchitectonic labeling approach for optically cleared human cortex samples, which can be applied to large (up to 5 mm thick) formalin fixed adult brain samples. A suite of small-molecule fluorescent nuclear and cytoplasmic dye protocols in combination with new refractive index matching solutions allows deep volume imaging. This greatly reduces time and cost of imaging cytoarchitecture in thick samples and enables classification of cytoarchitectonic layers over the full cortical depth. We demonstrate application of MASH to large archival samples of human visual areas, characterizing cortical architecture in 3D from the scale of cortical areas to that of single cells. In combination with scalable light sheet imaging and data analysis, MASH could open the door to investigation of large human cortical systems at cellular resolution and in the context of their complex 3-dimensional geometry.
Keywords	Medicine ; R ; Science ; Q
Subject code	616
Language	English
Publishing date	2019-07-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.

Hildebrand, Sven / Schueth, Anna / Wangenheim, Klaus von / Mattheyer, Christian / Pampaloni, Francesco / Bratzke, Hansjürgen / Roebroeck, Alard F / Galuske, Ralf A W

Scientific reports

2020 Volume 10, Issue 1, Page(s) 9950

Abstract: Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). ... ...

Abstract	Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
MeSH term(s)	Affinity Labels/chemistry ; Animals ; Brain/anatomy & histology ; Brain/cytology ; Carbocyanines/chemistry ; Fluorescent Dyes/chemistry ; Formaldehyde/chemistry ; Humans ; Lipids/chemistry ; Lysine/chemistry ; Mice ; Periodic Acid/chemistry ; Swine ; Tissue Fixation/methods
Chemical Substances	Affinity Labels ; Carbocyanines ; Fluorescent Dyes ; Lipids ; periodate-lysine-paraformaldehyde ; Periodic Acid (10450-60-9) ; Formaldehyde (1HG84L3525) ; 3,3'-dioctadecylindocarbocyanine (40957-95-7) ; Lysine (K3Z4F929H6)
Language	English
Publishing date	2020-06-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-66999-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Computer-assisted three-dimensional tracking of sensory innervation in the murine bladder mucosa with two-photon microscopy.

Schueth, Anna / Spronck, Bart / van Zandvoort, Marc A M J / van Koeveringe, Gommert A

Journal of chemical neuroanatomy

2017 Volume 85, Page(s) 43–49

Abstract: A strong association between functional bladder disorders and bladder sensation is well-known, with a relationship between malfunctioning detrusor muscle and abnormal sensation arising from the sub-urothelium and the lamina propria (LP), has been ... ...

Abstract	A strong association between functional bladder disorders and bladder sensation is well-known, with a relationship between malfunctioning detrusor muscle and abnormal sensation arising from the sub-urothelium and the lamina propria (LP), has been suggested. However, the exact underlying pathophysiology of these bladder disorders is not completely understood. Therefore, it is important to gain knowledge on sensory innervation of the urinary bladder in order to understand the neural network function in healthy and diseased bladder. In the present study we aim at the development of a computer-assisted method for 3D-tracking of sensory innervation in the murine bladder mucosa using two-photon laser scanning microscopy (TPLSM). TPLSM was performed on 10 fixed, stained (CGRP) bladder samples in both the trigone and dome. Nerve tracking was performed in subvolumes (6.3±2.910
MeSH term(s)	Animals ; Image Processing, Computer-Assisted ; Mice ; Microscopy ; Microscopy, Confocal ; Mucous Membrane ; Sensory Receptor Cells/physiology ; Urinary Bladder/innervation ; Urothelium/innervation
Language	English
Publishing date	2017-11
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	639443-7
ISSN	1873-6300 ; 0891-0618
ISSN (online)	1873-6300
ISSN	0891-0618
DOI	10.1016/j.jchemneu.2017.06.006
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Article ; Online: Age-related changes in murine bladder structure and sensory innervation: a multiphoton microscopy quantitative analysis.

Schueth, Anna / Spronck, Bart / van Zandvoort, Marc A M J / van Koeveringe, Gommert A

Age (Dordrecht, Netherlands)

2016 Volume 38, Issue 1, Page(s) 17

Abstract: Our study aimed to examine and quantify age-related structural alterations in the healthy mouse bladder using ex vivo two-photon laser scanning microscopy (TPLSM). Freshly dissected bladders from 25-, 52-, and 85-week-old C57bl/6J mice were examined, and ...

Abstract	Our study aimed to examine and quantify age-related structural alterations in the healthy mouse bladder using ex vivo two-photon laser scanning microscopy (TPLSM). Freshly dissected bladders from 25-, 52-, and 85-week-old C57bl/6J mice were examined, and morphological analyses and quantification of cell layers and nerves were performed. The numbers of stretched, curled, branched, and total number of nerves in volume units of the stained muscle layer were quantified. We observed differences in the bladder wall architecture and innervation with age. Especially in 85-week-old mice, age-related changes were found, including detachment of urothelial cells and an increase in connective tissue, intermingled with the smooth muscle fibers in the muscle layer (collagen-smooth muscle ratio of 1.15 ± 0.29). In 25- and 52-week-old mice, the collagen-smooth muscle ratios were 0.20 ± 0.04 and 0.31 ± 0.11, respectively, and a clear separation of collagen and muscle was observed. The overall number of nerves and the number of curled nerves were significantly higher in the 85-week-old mice (74.0 ± 13.0 and 25.9 ± 4.8, respectively), when comparing to 25-week-old mice (26.0 ± 2.7 and 6.7 ± 1.2, respectively) and 52-week-old mice (43.8 ± 4.3 and 22.1 ± 3.3, respectively). Significant age-related alterations in bladder morphology and innervation were found, when comparing freshly dissected bladder tissue from 25-, 52-, and 85-week-old mice. The higher number of curled nerves might be an indication of an increased neurotransmitter release, resulting in a higher nerve activity, with a part of the nerves being possibly mechanically impaired. This study shows that two-photon laser scanning microscopy of healthy aging male mice is a useful method to investigate and quantify the age-related changes in the bladder wall.
MeSH term(s)	Aging ; Animals ; Male ; Mice ; Mice, Inbred C57BL ; Microscopy, Fluorescence, Multiphoton/methods ; Muscle, Smooth/innervation ; Muscle, Smooth/ultrastructure ; Sensory Receptor Cells/ultrastructure ; Urinary Bladder/innervation ; Urinary Bladder/ultrastructure
Language	English
Publishing date	2016-02
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	423714-6
ISSN	1574-4647 ; 0161-9152
ISSN (online)	1574-4647
ISSN	0161-9152
DOI	10.1007/s11357-016-9878-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Murine bladder imaging by 2-photon microscopy: an experimental study of morphology.

Schueth, Anna / van Zandvoort, Marc A M J / Buurman, Wim A / van Koeveringe, Gommert A

The Journal of urology

2014 Volume 192, Issue 3, Page(s) 973–980

Abstract: Purpose: We developed 2-photon laser scanning microscopy analysis of the native murine bladder.: Materials and methods: Bladder tissue from wild-type mice was imaged by 2-photon laser scanning microscopy autofluorescence and second harmonic ... ...

Abstract	Purpose: We developed 2-photon laser scanning microscopy analysis of the native murine bladder. Materials and methods: Bladder tissue from wild-type mice was imaged by 2-photon laser scanning microscopy autofluorescence and second harmonic generation microscopy. Bladder wall layers and structures were analyzed using differences in color, size, shape and morphology. Results: Autofluorescence of the urothelium, nerve structures and muscles was visible in the green spectral channel due to autofluorochromes such as NAD(P)H and elastin. Second harmonic generation of collagen was seen in the blue spectral channel. Imaging from the mucosal side revealed umbrella cells at 0 and 30 μm, of which the high cellular NAD(P)H content allows autofluorescence detection. Below that a network-like connective tissue layer was visualized up to 50 μm that contained vessels with a diameter of 10 to 40 μm and nerves with a diameter of 1 to 6 μm. Imaging from the adventitial side revealed a radiant collagen layer covered with nerves and macrophages at 0 to 20 μm. Below at 20 to 25 μm we visualized a thick muscle layer containing elastic fibers and macrophages. Findings were also represented in 3-dimensional reconstructions, providing information on structure localization, orientation and interconnection. Conclusions: Two-photon laser scanning microscopy imaging using autofluorescence of the murine bladder is a promising technique to provide new insight into structures and morphology. It opens avenues to identify structural changes in bladder pathology.
MeSH term(s)	Animals ; Mice ; Microscopy, Confocal ; Urinary Bladder/anatomy & histology
Language	English
Publishing date	2014-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3176-8
ISSN	1527-3792 ; 0022-5347
ISSN (online)	1527-3792
ISSN	0022-5347
DOI	10.1016/j.juro.2014.03.103
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