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Article ; Online: Lian-Mei-Yin formula alleviates diet-induced hepatic steatosis by suppressing Yap1/FOXM1 pathway-dependent lipid synthesis.

Zhang, Peiguang / Cao, Jieqiong / Liang, Xujing / Su, Zijian / Zhang, Bihui / Wang, Zhenyu / Xie, Junye / Chen, Gengrui / Chen, Xue / Zhang, Jinting / Feng, Yanxian / Xu, Qin / Song, Jianping / Hong, An / Chen, Xiaojia / Zhang, Yibo

Acta biochimica et biophysica Sinica

2024 Volume 56, Issue 4, Page(s) 621–633

Abstract: ... therapeutic agents for NAFLD. Lian-Mei-Yin (LMY) is a traditional Chinese medicine formula used for decades ...

Abstract	Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, with a global prevalence of 25%. Patients with NAFLD are more likely to suffer from advanced liver disease, cardiovascular disease, or type II diabetes. However, unfortunately, there is still a shortage of FDA-approved therapeutic agents for NAFLD. Lian-Mei-Yin (LMY) is a traditional Chinese medicine formula used for decades to treat liver disorders. It has recently been applied to type II diabetes which is closely related to insulin resistance. Given that NAFLD is another disease involved in insulin resistance, we hypothesize that LMY might be a promising formula for NAFLD therapy. Herein, we verify that the LMY formula effectively reduces hepatic steatosis in diet-induced zebrafish and NAFLD model mice in a time- and dose-dependent manner. Mechanistically, LMY suppresses Yap1-mediated Foxm1 activation, which is crucial for the occurrence and development of NAFLD. Consequently, lipogenesis is ameliorated by LMY administration. In summary, the LMY formula alleviates diet-induced NAFLD in zebrafish and mice by inhibiting Yap1/Foxm1 signaling-mediated NAFLD pathology.
MeSH term(s)	Humans ; Mice ; Animals ; Non-alcoholic Fatty Liver Disease/drug therapy ; Non-alcoholic Fatty Liver Disease/etiology ; Non-alcoholic Fatty Liver Disease/metabolism ; Lipogenesis ; Zebrafish ; Insulin Resistance ; Diabetes Mellitus, Type 2/metabolism ; Liver/metabolism ; Diet, High-Fat ; Transcription Factors/metabolism ; Adaptor Proteins, Signal Transducing/metabolism ; Lipids ; Mice, Inbred C57BL ; Forkhead Box Protein M1/metabolism
Chemical Substances	Transcription Factors ; Adaptor Proteins, Signal Transducing ; Lipids ; FOXM1 protein, human ; Forkhead Box Protein M1
Language	English
Publishing date	2024-03-21
Publishing country	China
Document type	Journal Article
ZDB-ID	2175256-4
ISSN	1745-7270 ; 0582-9879 ; 1672-9145
ISSN (online)	1745-7270
ISSN	0582-9879 ; 1672-9145
DOI	10.3724/abbs.2024025
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Article ; Online: Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation.

Wu, Fan / Shao, Qingqing / Hu, Meilin / Zhao, Yan / Dong, Ruolan / Fang, Ke / Xu, Lijun / Zou, Xin / Lu, Fuer / Li, Jingbin / Chen, Guang

Journal of ethnopharmacology

2020 Volume 252, Page(s) 112580

Abstract: Ethnopharmacological relevance: Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is ...

Abstract	Ethnopharmacological relevance: Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is one of the most important formulations to treat digestive diseases from ancient times to the present. Previous study showed that WMW has satisfactory curative effects on experimental colitis, which motivating the application of WMW on colitis-associated complications. Aim of the study: Intestinal fibrosis is usually considered to be a common complication of inflammatory bowel disease (IBD), particularly Crohn's disease (CD). Currently, no effective preventive measures or medical therapies are available for that. This work was designed to evaluate the effect and related mechanism of WMW on chronic colitis-associated intestinal fibrosis mice model. Materials and methods: The chronic colitis-associated intestinal fibrosis mice model was established by weekly intrarectal injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The mice survival rate, disease activity index (DAI), colon length and histological score were examined to assess the therapeutic effect of WMW. Masson's trichrome staining, hydroxyproline assay, immunohistochemical staining and western blot analysis were used to evaluate fibrosis level. Colon inflammation was determined by ELISA and immunofluorescence staining. Immunofluorescence staining was used to evaluate fibroblasts proliferation and epithelial to mesenchymal transition (EMT), and the expression of key molecules in fibrosis was analyzed by western blot. Results: Here we showed that WMW alleviates chronic colitis with improved survival rate, DAI, colon length and histological score. WMW inhibited the progression of intestinal fibrosis, decreased the expression of various fibrosis markers, such as α-SMA, collagen I, MMP-9 and fibronectin. In addition, WMW treatment reduced cytokines IL-6 and IFN-γ, and downregulated proinflammatory NF-κBp65 and STAT3 signaling pathways. Importantly, administration of WMW led to the inhibition of colon fibroblast proliferation and EMT, which are important mediators during fibrosis. Several key profibrotic pathways, including TGF-β/Smad and Wnt/β-catenin pathways, were downregulated by WMW treatment. Conclusion: Our work demonstrated that WMW can prevent intestinal fibrosis and the mechanisms involved may be related to the inhibition of colon fibroblasts activation.
MeSH term(s)	Animals ; Chronic Disease ; Colitis/complications ; Colitis/drug therapy ; Colitis/immunology ; Colitis/pathology ; Colon/drug effects ; Colon/immunology ; Colon/pathology ; Cytokines/blood ; Cytokines/immunology ; Fibroblasts/drug effects ; Fibrosis ; Male ; Medicine, Chinese Traditional ; Mice, Inbred C57BL
Chemical Substances	Cytokines
Language	English
Publishing date	2020-01-20
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.112580
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Article: Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.

Wu, Fan / Shao, Qingqing / Cheng, Zhe / Xiong, Xinyu / Fang, Ke / Zhao, Yan / Dong, Ruolan / Xu, Lijun / Lu, Fuer / Chen, Guang

Chinese medicine

2021 Volume 16, Issue 1, Page(s) 78

Abstract: ... a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW ...

Abstract	Background: Accumulating evidence indicated that necroptosis plays an essential role in the pathogenesis of inflammatory bowel disease (IBD). The O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) of necroptotic signal molecule receptor-interacting serine-threonine kinase 3 (RIPK3) was reported to exert a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW) as an effective prescription in mouse colitis. However, the potential mechanisms are not fully understood. Considering the crucial role of necroptosis in the pathogenesis of IBD, therefore, this study was designed to explain whether the anti-colitis effect of WMW is mediated by modulating necroptosis and its related mechanisms. Methods: The protective effects of WMW on colitis have been determined by detecting colitis mice body weight, disease activity index (DAI), survival rate and colon length. Colonic inflammation was examined by inflammatory cells infiltration and local cytokines levels. After then, we measured the levels of necroptosis and O-GlcNAcylation. C O-immunoprecipitation experiments were used to address whether elevated O-GlcNAcylation can inhibit necroptotic signal transduction in the treatment of WMW. Finally, the key enzymes in O-GlcNAcylation: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were examined and molecular docking analysis was used to determine effective natural compounds in the regulation on OGT and OGA activities. Results: Our results showed that WMW significantly improved mice body weight, survival rate and colon length, decreased DAI in TNBS-induced colitis. WMW obviously alleviated colonic inflammatory responses with reduced macrophages, neutrophils infiltration and local IL-1β, IL-6, TNF-α and IFN-γ levels. It was found that WMW increased colonic O-GlcNAcylation level and inhibited the activation of RIPK1, RIPK3 and MLKL. Then, further experiments revealed that WMW enhanced OGT activity and suppressed OGA activity, thereby increasing RIPK3 O-GlcNAcylation and inhibiting the binding of RIPK3 and MLKL, which led to the inhibition of necroptosis. Additionally, docking analysis demonstrated that hesperidin, coptisine and ginsenoside Rb1 may exert a major role in the regulation on OGT and OGA activities by WMW. Conclusion: Our work demonstrated that WMW can alleviate TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
Language	English
Publishing date	2021-08-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2260322-0
ISSN	1749-8546
ISSN	1749-8546
DOI	10.1186/s13020-021-00493-4
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Article: ‘Cao Mei Tang’: A New Pink Candy Lily (Iris ×norrisii) Cultivar

Gao, Jingli / Yu, Fengyang / Xu, Wenji / Xiao, Yuee / Bi, Xiaoying

HortScience. 2020 Aug., v. 55, no. 8

2020

Abstract: ... in The American Iris Society (2020). ‘Cao Mei Tang’ is a Candy Lily cultivar developed by Shenyang Agricultural ... per plant than any other species. It is easy to grow and is tolerant of drought and barren conditions (Xu et al., 2017; Zheng et al ... in 1967 (Bi et al., 2012; Lenz, 1972; Xu et al., 2017). There are 60 Candy Lily cultivars registered ...

Abstract	Candy Lily is the common name of Iris ×norrisii, which is a hybrid between the Vesper Iris (I. dichotoma) and the Blackberry Lily (I. domestica). Not only do the flowers of Candy Lily display an astonishing range of colors, but also, interestingly, after the blossoms wither, its petals twist themselves into tight little spirals that add fun and a beauty of their own, extending the ornamental display in the garden. Its common name, Candy Lily, comes from the resemblance of these spirals to a candy cane. It flowers in long midsummer days when no other irises are in bloom, and produces more buds per plant than any other species. It is easy to grow and is tolerant of drought and barren conditions (Xu et al., 2017; Zheng et al., 2017). Because of these characteristics, it is widely grown in horticulture (Whitehouse, 2011) and has become more and more attractive as a new floricultural crop since it was first raised by Samuel Norris in 1967 (Bi et al., 2012; Lenz, 1972; Xu et al., 2017). There are 60 Candy Lily cultivars registered in The American Iris Society (2020). ‘Cao Mei Tang’ is a Candy Lily cultivar developed by Shenyang Agricultural University in China to provide an attractive, summer-blooming pink iris for the dry and barren conditions in this hardiness zone.
Keywords	Iris domestica ; calcium oxide ; candy ; common names ; cultivars ; drought ; floriculture crops ; gardens ; hybrids ; summer
Language	English
Dates of publication	2020-08
Size	p. 1395-1396.
Publishing place	American Society for Horticultural Science
Document type	Article
Note	NAL-AP-2-clean
ISSN	2327-9834
DOI	10.21273/HORTSCI15065-20
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Wu-Mei-Wan prevents high-fat diet-induced obesity by reducing white adipose tissue and enhancing brown adipose tissue function.

Wu, Fan / Yang, Xueping / Hu, Meilin / Shao, Qingqing / Fang, Ke / Li, Jingbin / Zhao, Yan / Xu, Lijun / Zou, Xin / Lu, Fuer / Chen, Guang

Phytomedicine : international journal of phytotherapy and phytopharmacology

2020 Volume 76, Page(s) 153258

Abstract: Background: Wu-Mei-Wan, a classic traditional Chinese herb medicine, is one of the most important ...

Abstract	Background: Wu-Mei-Wan, a classic traditional Chinese herb medicine, is one of the most important formulations to treat digestive diseases from ancient times to the present. Our previous study showed that WMW treatment can prevent T2DM in db/db mice, which motivating the application of WMW on metabolic disorders. Purpose: Obesity and its comorbid diseases have increased dramatically and are now a worldwide health problem. There is still a lack of satisfactory treatment strategies for obesity. This work was designed to assess the effect and related mechanism of WMW on high fat diet (HFD)-induced obese mice model. Methods: Obese mice were induced by HFD. Thetherapeutic effect of WMW were analyzed by examining body and adipose tissue weight, metabolic profile and energy expenditure. Adipose tissue phenotype was determined by histological staining and the mitochondrial content was examined by transmission electron microscopy (TEM). Immunohistochemical and immunofluorescence staining, RT-qPCR and Western blot analysis were used to evaluate expression of key molecules in adipose tissue. Results: WMW treatment significantly protects HFD-induced obesity. Here we showed that WMW limits weight gain, improves metabolic profile and increases energy expenditure. WMW inhibits the hypertrophy and hyperplasia of white adipocytes, the mechanism involving the inhibition of TLR3/IL-6/JAK1/STAT3 pathway. In brown adipose tissue (BAT), WMW promotes thermogenicprogramme without affecting cell proliferation. The activated BMP7/ Smad1/5/9 pathway is considered to be one of the explanations for the effect of WMW on BAT. Conclusion: Our results suggested that WMW can prevent obesity and its underlying mechanisms are associated with reducing white adipose tissue and enhancing brown adipose tissue function.
Language	English
Publishing date	2020-06-03
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2020.153258
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Zs.A 4115: Show issues

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Article: Efficacy and Safety of Wei Bi Mei, a Chinese Herb Compound, as an Alternative to Bismuth for Eradication of

Li, Lei / Meng, FanDong / Zhu, Shengtao / Guo, ShuiLong / Wang, YongJun / Zhao, Xin / Sun, YiLin / Zhang, Yan / Wang, QinQin / Xu, HuFeng / Zhang, ShuTian

Evidence-based complementary and alternative medicine : eCAM

2018 Volume 2018, Page(s) 4320219

Abstract: ... been raised. We first assessed the efficacy and safety of Wei Bi Mei granules, which are bismuth ...

Abstract	Bismuth-containing quadruple therapy has been recommended as the first line of treatment in areas of high clarithromycin or metronidazole resistance. However, safety concerns of bismuth agents have long been raised. We first assessed the efficacy and safety of Wei Bi Mei granules, which are bismuth compounds consisting of three synthetic drugs and five medicinal herbs, compared to bismuth aluminate and colloidal bismuth subcitrate (CBS) in
Language	English
Publishing date	2018-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2018/4320219
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Zs.A 5995: Show issues

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Article ; Online: Production of alkaline pectinase: a case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis CAS-MEI-2-33.

Zhang, Ge / Li, Shugui / Xu, Yingbo / Wang, Juan / Wang, Fan / Xin, Yuhua / Shen, Zhong / Zhang, Haibo / Ma, Ming / Liu, Haobao

BMC biotechnology

2019 Volume 19, Issue 1, Page(s) 45

Abstract: ... hazardous to the environment.: Results: In this study, Bacillus tequilensis CAS-MEI-2-33 was isolated from cigar wrappers ... for the production of pectinase by B. tequilensis CAS-MEI-2-33 were optimized. The optimal fermentation period, pH ... of the initial fermentation medium, concentration of TS, and inoculum amount for B. tequilensis CAS-MEI-2-33 were ...

Abstract	Background: Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environment. Results: In this study, Bacillus tequilensis CAS-MEI-2-33 was isolated from cigar wrappers to produce alkaline pectinase using TS. Subsequently, the medium and fermentation conditions for the production of pectinase by B. tequilensis CAS-MEI-2-33 were optimized. The optimal fermentation period, pH of the initial fermentation medium, concentration of TS, and inoculum amount for B. tequilensis CAS-MEI-2-33 were 40 h, 40 g/L, 7.0, and 3%, respectively. Under optimal conditions, the pectinase activity was 1370 U/mL. Then, the enzymatic properties, such as the optimum pH, reaction temperature, temperature stability, and effects of metal ions, were studied. The optimal pH was determined to be 10.0, indicating that the enzyme was an alkaline pectinase. The optimal temperature was 40 °C, and pectinase activity was stable at 40 °C. The Ag Conclusions: This study provided a new alkaline pectinase candidate and a new strategy for the use of TS.
MeSH term(s)	Bacillus/classification ; Bacillus/enzymology ; Bacillus/genetics ; Bacterial Proteins/metabolism ; Enzyme Stability ; Fermentation ; Hydrogen-Ion Concentration ; Industrial Microbiology/methods ; Pectins/metabolism ; Phylogeny ; Polygalacturonase/metabolism ; Temperature ; Nicotiana/chemistry ; Nicotiana/metabolism ; Tobacco Products/microbiology
Chemical Substances	Bacterial Proteins ; Pectins (89NA02M4RX) ; Polygalacturonase (EC 3.2.1.15)
Language	English
Publishing date	2019-07-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2052746-9
ISSN	1472-6750 ; 1472-6750
ISSN (online)	1472-6750
ISSN	1472-6750
DOI	10.1186/s12896-019-0526-6
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Article: Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation

Wu, Fan / Chen, Guang / Dong, Ruolan / Fang, Ke / Hu, Meilin / Li, Jingbin / Lu, Fuer / Shao, Qingqing / Xu, Lijun / Zhao, Yan / Zou, Xin

Journal of ethnopharmacology. 2020 Apr. 24, v. 252

2020

Abstract: Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is one of the most important ...

Abstract	Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is one of the most important formulations to treat digestive diseases from ancient times to the present. Previous study showed that WMW has satisfactory curative effects on experimental colitis, which motivating the application of WMW on colitis-associated complications.Intestinal fibrosis is usually considered to be a common complication of inflammatory bowel disease (IBD), particularly Crohn's disease (CD). Currently, no effective preventive measures or medical therapies are available for that. This work was designed to evaluate the effect and related mechanism of WMW on chronic colitis-associated intestinal fibrosis mice model.The chronic colitis-associated intestinal fibrosis mice model was established by weekly intrarectal injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The mice survival rate, disease activity index (DAI), colon length and histological score were examined to assess the therapeutic effect of WMW. Masson's trichrome staining, hydroxyproline assay, immunohistochemical staining and western blot analysis were used to evaluate fibrosis level. Colon inflammation was determined by ELISA and immunofluorescence staining. Immunofluorescence staining was used to evaluate fibroblasts proliferation and epithelial to mesenchymal transition (EMT), and the expression of key molecules in fibrosis was analyzed by western blot.Here we showed that WMW alleviates chronic colitis with improved survival rate, DAI, colon length and histological score. WMW inhibited the progression of intestinal fibrosis, decreased the expression of various fibrosis markers, such as α-SMA, collagen I, MMP-9 and fibronectin. In addition, WMW treatment reduced cytokines IL-6 and IFN-γ, and downregulated proinflammatory NF-κBp65 and STAT3 signaling pathways. Importantly, administration of WMW led to the inhibition of colon fibroblast proliferation and EMT, which are important mediators during fibrosis. Several key profibrotic pathways, including TGF-β/Smad and Wnt/β-catenin pathways, were downregulated by WMW treatment.Our work demonstrated that WMW can prevent intestinal fibrosis and the mechanisms involved may be related to the inhibition of colon fibroblasts activation.
Keywords	animal models ; beta catenin ; cell proliferation ; colitis ; collagen ; colon ; Crohn disease ; enzyme-linked immunosorbent assay ; epithelium ; fibroblasts ; fibronectins ; fibrosis ; fluorescent antibody technique ; hydroxyproline ; immunohistochemistry ; inflammation ; interferon-gamma ; interleukin-6 ; mice ; Oriental traditional medicine ; signal transduction ; staining ; sulfonic acids ; survival rate ; therapeutics ; transforming growth factor beta ; Western blotting
Language	English
Dates of publication	2020-0424
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.112580
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation

Fan Wu / Qingqing Shao / Zhe Cheng / Xinyu Xiong / Ke Fang / Yan Zhao / Ruolan Dong / Lijun Xu / Fuer Lu / Guang Chen

Chinese Medicine, Vol 16, Iss 1, Pp 1-

2021 Volume 17

Abstract: ... a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW ...

Abstract	Abstract Background Accumulating evidence indicated that necroptosis plays an essential role in the pathogenesis of inflammatory bowel disease (IBD). The O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) of necroptotic signal molecule receptor-interacting serine-threonine kinase 3 (RIPK3) was reported to exert a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW) as an effective prescription in mouse colitis. However, the potential mechanisms are not fully understood. Considering the crucial role of necroptosis in the pathogenesis of IBD, therefore, this study was designed to explain whether the anti-colitis effect of WMW is mediated by modulating necroptosis and its related mechanisms. Methods The protective effects of WMW on colitis have been determined by detecting colitis mice body weight, disease activity index (DAI), survival rate and colon length. Colonic inflammation was examined by inflammatory cells infiltration and local cytokines levels. After then, we measured the levels of necroptosis and O-GlcNAcylation. C O-immunoprecipitation experiments were used to address whether elevated O-GlcNAcylation can inhibit necroptotic signal transduction in the treatment of WMW. Finally, the key enzymes in O-GlcNAcylation: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were examined and molecular docking analysis was used to determine effective natural compounds in the regulation on OGT and OGA activities. Results Our results showed that WMW significantly improved mice body weight, survival rate and colon length, decreased DAI in TNBS-induced colitis. WMW obviously alleviated colonic inflammatory responses with reduced macrophages, neutrophils infiltration and local IL-1β, IL-6, TNF-α and IFN-γ levels. It was found that WMW increased colonic O-GlcNAcylation level and inhibited the activation of RIPK1, RIPK3 and MLKL. Then, further experiments revealed that WMW enhanced OGT activity and suppressed OGA activity, thereby increasing RIPK3 ...
Keywords	Wu-Mei-Wan ; Colitis ; Necroptosis ; O-GlcNAcylation ; RIPK3 ; Other systems of medicine ; RZ201-999
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
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Article: Fructus Mume (Wu Mei) Attenuates Acetic Acid-Induced Ulcerative Colitis by Regulating Inflammatory Cytokine, Reactive Oxygen Species, and Neuropeptide Levels in Model Rats

Xu, Zongying / Zhang, Xueli / Wang, Wenya / Zhang, Di / Ma, Yan / Zhang, Dongmei / Chen, Meng

Journal of medicinal food. 2022 Apr. 01, v. 25, no. 4

2022

Abstract: Ulcerative colitis (UC) is a chronic idiopathic inflammatory disorder of the large intestine. Fructus mume (FM), a natural food with nutritive and pharmaceutical value, has demonstrated therapeutic efficacy against UC. In this study, we investigated the ... ...

Abstract	Ulcerative colitis (UC) is a chronic idiopathic inflammatory disorder of the large intestine. Fructus mume (FM), a natural food with nutritive and pharmaceutical value, has demonstrated therapeutic efficacy against UC. In this study, we investigated the protective effects and mechanisms of FM against UC. We induced UC in rats with 4% (v/v) acetic acid (AA), orally administered 0.7 or 0.325 g/kg FM and 0.3 g/kg sulfasalazine (SASP) for 7 days, and explored the responses the drugs elicited in the rats. We assessed the general conditions of the rats by the disease active index. We evaluated colon tissue damage macroscopically and by Hematoxylin & Eosin, Alcian Blue–periodic acid–Schiff, and Masson's staining, and explored the potential mechanisms of FM on inflammation, oxidative stress, and neuropeptides by measuring TNF-α, IL-6, IL-8, IL-10, MMP9, CXCR-1, SOD, GSH-px, MDA, ROS, SIRT3, SP, VIP, ghrelin, and 5-HT. FM treatment significantly attenuated colon damage and submucosal fibrosis compared with the model. It lowered serum proinflammatory TNF-α, IL-8, and colonic MMP9 and CXCR-1, and raised serum anti-inflammatory IL-10 levels. FM upregulated the antioxidant enzymes SOD, GSH-px, and SITR3 protein but inhibited ROS and MDA production. It downregulated colonic SP, VIP, ghrelin, and 5-HT. The beneficial effects of FM might be dose dependent. Around 0.7 g/kg FM and SASP displayed similar efficacy for treating AA-induced colitis in rats. Our results provide empirical evidence that FM protects against AA-induced UC in rats via anti-inflammatory and antioxidant mechanisms, and regulates neuropeptides; thus, FM may be a promising, safe, and efficacious alternative therapy for UC, if its efficacy can be confirmed in human trials.
Keywords	acetic acid ; blood serum ; colon ; dose response ; eosin ; fibrosis ; functional foods ; ghrelin ; humans ; inflammation ; interleukin-10 ; interleukin-6 ; interleukin-8 ; models ; neuropeptides ; oxidative stress ; reactive oxygen species ; serotonin ; sulfasalazine ; ulcerative colitis
Language	English
Dates of publication	2022-0401
Size	p. 389-401.
Publishing place	Mary Ann Liebert, Inc.
Document type	Article
ZDB-ID	1427365-2
ISSN	1557-7600 ; 1096-620X
ISSN (online)	1557-7600
ISSN	1096-620X
DOI	10.1089/jmf.2021.K.0155
Database	NAL-Catalogue (AGRICOLA)

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