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  1. Article ; Online: Correction: An Endophytic Fungus, Talaromyces radicus, Isolated from Catharanthus roseus, Produces Vincristine and Vinblastine, Which Induce Apoptotic Cell Death.

    Palem, Padmini P C / Kuriakose, Gini C / Jayabaskaran, Chelliah

    PloS one

    2016  Volume 11, Issue 4, Page(s) e0153111

    Language English
    Publishing date 2016
    Publishing country United States
    Document type Published Erratum
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0153111
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Fungal vincristine from Eutypella spp - CrP14 isolated from Catharanthus roseus induces apoptosis in human squamous carcinoma cell line -A431.

    Kuriakose, Gini C / Palem, Padmini P C / Jayabaskaran, Chelliah

    BMC complementary and alternative medicine

    2016  Volume 16, Issue 1, Page(s) 302

    Abstract: Background: Catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. Previously we have reported that Eutypella spp - CrP14 isolated from stem cutting of this ... ...

    Abstract Background: Catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. Previously we have reported that Eutypella spp - CrP14 isolated from stem cutting of this plant had shown significant antiproliferative activity when tested in vitro against HeLa cell line. The present study was conducted to identify the anticancer compound responsible for the anti-proliferative activity of the fungal extract and to evaluate its in vitro anticancer and apoptotic effects.
    Methods: The anti-proliferative activity of the fungal anticancer compound, vincristine was analyzed by MTT assay against different cancer cell lines. We examined its efficacy of apoptotic induction on A431 cells. The parameters examined included cell cycle distribution, loss of mitochondrial membrane potential (MMP), DNA fragmentation and reactive oxygen species (ROS) generation.
    Results: The presence of vincristine in fungal culture filtrate was confirmed through chromatographic and spectroscopic analyses, and the amount was estimated to be 53 ± 5.0 μg/l. The partially purified fungal vincristine had strong cytotoxic activity towards human squamous carcinoma cells - A431 in the MTT assay. Furthermore, we showed that the fungal vincristine was capable of inducing apoptosis in A431 cells through generation of reactive oxygen species and activation of the intrinsic pathway leading to loss of MMP.
    Conclusions: We have demonstrated for the first time that the vincristine from Eutypella spp - CrP14 is an efficient inducer of apoptosis in A431 cells, meriting its further evaluation in vivo.
    MeSH term(s) Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Carcinoma, Squamous Cell ; Catharanthus/microbiology ; Cell Line, Tumor ; Humans ; Vincristine/chemistry ; Vincristine/pharmacology ; Xylariales/chemistry
    Chemical Substances Antineoplastic Agents ; Vincristine (5J49Q6B70F)
    Language English
    Publishing date 2016-08-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2050429-9
    ISSN 1472-6882 ; 1472-6882
    ISSN (online) 1472-6882
    ISSN 1472-6882
    DOI 10.1186/s12906-016-1299-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Correction

    Padmini P C Palem / Gini C Kuriakose / Chelliah Jayabaskaran

    PLoS ONE, Vol 11, Iss 4, p e

    An Endophytic Fungus, Talaromyces radicus, Isolated from Catharanthus roseus, Produces Vincristine and Vinblastine, Which Induce Apoptotic Cell Death.

    2016  Volume 0153111

    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2016-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  4. Article ; Online: An Endophytic Fungus, Talaromyces radicus, Isolated from Catharanthus roseus, Produces Vincristine and Vinblastine, Which Induce Apoptotic Cell Death.

    Palem, Padmini P C / Kuriakose, Gini C / Jayabaskaran, Chelliah

    PloS one

    2015  Volume 10, Issue 12, Page(s) e0144476

    Abstract: ... and vinblastine. Twenty-two endophytic fungi isolated from various tissues of C. roseus were ...

    Abstract Endophytic fungi isolated from Catharanthus roseus were screened for the production of vincristine and vinblastine. Twenty-two endophytic fungi isolated from various tissues of C. roseus were characterized taxonomically by sequence analysis of the internal transcribed spacer (ITS) region of rDNA and grouped into 10 genera: Alternaria, Aspergillus, Chaetomium, Colletotrichum, Dothideomycetes, Eutypella, Eutypa, Flavodon, Fusarium and Talaromyces. The antiproliferative activity of these fungi was assayed in HeLa cells using the MTT assay. The fungal isolates Eutypella sp--CrP14, obtained from stem tissues, and Talaromyces radicus--CrP20, obtained from leaf tissues, showed the strongest antiproliferative activity, with IC50 values of 13.5 μg/ml and 20 μg/ml, respectively. All 22 endophytic fungi were screened for the presence of the gene encoding tryptophan decarboxylase (TDC), the key enzyme in the terpenoid indole alkaloid biosynthetic pathway, though this gene could only be amplified from T. radicus--CrP20 (NCBI GenBank accession number KC920846). The production of vincristine and vinblastine by T. radicus--CrP20 was confirmed and optimized in nine different liquid media. Good yields of vincristine (670 μg/l) in modified M2 medium and of vinblastine (70 μg/l) in potato dextrose broth medium were obtained. The cytotoxic activity of partially purified fungal vincristine was evaluated in different human cancer cell lines, with HeLa cells showing maximum susceptibility. The apoptosis-inducing activity of vincristine derived from this fungus was established through cell cycle analysis, loss of mitochondrial membrane potential and DNA fragmentation patterns.
    MeSH term(s) Apoptosis/drug effects ; Catharanthus/microbiology ; Cell Cycle/drug effects ; Cell Proliferation/drug effects ; DNA Fragmentation/drug effects ; HeLa Cells ; Humans ; Membrane Potential, Mitochondrial/drug effects ; Talaromyces/chemistry ; Talaromyces/isolation & purification ; Talaromyces/metabolism ; Vinblastine/isolation & purification ; Vinblastine/metabolism ; Vinblastine/pharmacology ; Vincristine/isolation & purification ; Vincristine/metabolism ; Vincristine/pharmacology
    Chemical Substances Vincristine (5J49Q6B70F) ; Vinblastine (5V9KLZ54CY)
    Language English
    Publishing date 2015-12-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0144476
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: An Endophytic Fungus, Talaromyces radicus, Isolated from Catharanthus roseus, Produces Vincristine and Vinblastine, Which Induce Apoptotic Cell Death.

    Padmini P C Palem / Gini C Kuriakose / Chelliah Jayabaskaran

    PLoS ONE, Vol 10, Iss 12, p e

    2015  Volume 0144476

    Abstract: ... and vinblastine. Twenty-two endophytic fungi isolated from various tissues of C. roseus were ...

    Abstract Endophytic fungi isolated from Catharanthus roseus were screened for the production of vincristine and vinblastine. Twenty-two endophytic fungi isolated from various tissues of C. roseus were characterized taxonomically by sequence analysis of the internal transcribed spacer (ITS) region of rDNA and grouped into 10 genera: Alternaria, Aspergillus, Chaetomium, Colletotrichum, Dothideomycetes, Eutypella, Eutypa, Flavodon, Fusarium and Talaromyces. The antiproliferative activity of these fungi was assayed in HeLa cells using the MTT assay. The fungal isolates Eutypella sp--CrP14, obtained from stem tissues, and Talaromyces radicus--CrP20, obtained from leaf tissues, showed the strongest antiproliferative activity, with IC50 values of 13.5 μg/ml and 20 μg/ml, respectively. All 22 endophytic fungi were screened for the presence of the gene encoding tryptophan decarboxylase (TDC), the key enzyme in the terpenoid indole alkaloid biosynthetic pathway, though this gene could only be amplified from T. radicus--CrP20 (NCBI GenBank accession number KC920846). The production of vincristine and vinblastine by T. radicus--CrP20 was confirmed and optimized in nine different liquid media. Good yields of vincristine (670 μg/l) in modified M2 medium and of vinblastine (70 μg/l) in potato dextrose broth medium were obtained. The cytotoxic activity of partially purified fungal vincristine was evaluated in different human cancer cell lines, with HeLa cells showing maximum susceptibility. The apoptosis-inducing activity of vincristine derived from this fungus was established through cell cycle analysis, loss of mitochondrial membrane potential and DNA fragmentation patterns.
    Keywords Medicine ; R ; Science ; Q
    Subject code 571
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Fungal vincristine from Eutypella spp - CrP14 isolated from Catharanthus roseus induces apoptosis in human squamous carcinoma cell line -A431

    Kuriakose, Gini C / Padmini P. C. Palem / Chelliah Jayabaskaran

    BMC complementary and alternative medicine. 2016 Dec., v. 16, no. 1

    2016  

    Abstract: BACKGROUND: Catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. Previously we have reported that Eutypella spp – CrP14 isolated from stem cutting of this ... ...

    Abstract BACKGROUND: Catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. Previously we have reported that Eutypella spp – CrP14 isolated from stem cutting of this plant had shown significant antiproliferative activity when tested in vitro against HeLa cell line. The present study was conducted to identify the anticancer compound responsible for the anti-proliferative activity of the fungal extract and to evaluate its in vitro anticancer and apoptotic effects. METHODS: The anti-proliferative activity of the fungal anticancer compound, vincristine was analyzed by MTT assay against different cancer cell lines. We examined its efficacy of apoptotic induction on A431 cells. The parameters examined included cell cycle distribution, loss of mitochondrial membrane potential (MMP), DNA fragmentation and reactive oxygen species (ROS) generation. RESULTS: The presence of vincristine in fungal culture filtrate was confirmed through chromatographic and spectroscopic analyses, and the amount was estimated to be 53 ± 5.0 μg/l. The partially purified fungal vincristine had strong cytotoxic activity towards human squamous carcinoma cells – A431 in the MTT assay. Furthermore, we showed that the fungal vincristine was capable of inducing apoptosis in A431 cells through generation of reactive oxygen species and activation of the intrinsic pathway leading to loss of MMP. CONCLUSIONS: We have demonstrated for the first time that the vincristine from Eutypella spp – CrP14 is an efficient inducer of apoptosis in A431 cells, meriting its further evaluation in vivo.
    Keywords Catharanthus roseus ; DNA fragmentation ; Eutypella ; alternative medicine ; apoptosis ; cell cycle ; chromatography ; culture filtrates ; cutting ; cytotoxicity ; fungi ; humans ; medicinal plants ; membrane potential ; microbial culture ; mitochondrial membrane ; neoplasms ; reactive oxygen species ; secondary metabolites ; spectral analysis ; terpenoids ; vinblastine ; vincristine
    Language English
    Dates of publication 2016-12
    Size p. 302.
    Publishing place BioMed Central
    Document type Article
    ZDB-ID 2050429-9
    ISSN 1472-6882
    ISSN 1472-6882
    DOI 10.1186/s12906-016-1299-2
    Database NAL-Catalogue (AGRICOLA)

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