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  1. Thesis ; Online: A Review of Closed Canine Forensics DNA Case Reports from the University of California, Davis

    Scharnhorst, Gunther Konrad

    2010  

    Abstract: Canine biological materials including hair, dander, and saliva can be found when contact between dogs and humans takes place. As such, analysis of canine DNA portends critical links between suspects and victims in forensic investigations. Although canine ...

    Abstract Canine biological materials including hair, dander, and saliva can be found when contact between dogs and humans takes place. As such, analysis of canine DNA portends critical links between suspects and victims in forensic investigations. Although canine biomaterial can be equally as probative as human biological evidence, canine forensic DNA methodologies and resources are not as highly developed or standardized as those methods used in human forensics. Therefore, canine DNA evidence is typically used to provide investigative leads rather than as incriminating evidence. While a standardized panel of short tandem repeat (STR) and mitochondrial DNA (mtDNA) markers and publicly accessible genetic databases for canine forensic DNA analysis are already available, the persistent lack of supporting resources including accredited and generally accepted quality assurance (QA) and quality control (QC) programs (such as quality manuals, quality control, management, training and testing, and standard operating protocols [SOPs]) still plagues the animal forensic community. Furthermore, for government forensic laboratories, expertise and interest in using animal DNA, including analysis of canine biological evidence, has not yet become established. When little or no other traditional physical evidence is present, only then do investigators tend to resort to animal DNA testing if animal biomaterial is recovered. This research report examines and assesses the scientific and technical aspects of animal forensic testing at the University of California, Davis. It must be emphasized that this report focuses on closed canine cases from 2003-2005, but extends the scope of its review more widely to include other animal DNA forensic testing services. The findings and recommendations contained in this report are designed to objectively assess the past, evaluate the present, and recommend reforms that will assist the animal forensic science community in providing the best possible services that comply with court standards and bear judicial scrutiny.
    Keywords Forensic anthropology|Genetics|Criminology|Bioinformatics|Veterinary services
    Subject code 630
    Language ENG
    Publishing date 2010-01-01 00:00:01.0
    Publisher University of California, Davis
    Publishing country us
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: An assessment of scientific and technical aspects of closed investigations of canine forensics DNA--case series from the University of California, Davis, USA.

    Scharnhorst, Günther / Kanthaswamy, Sree

    Croatian medical journal

    2011  Volume 52, Issue 3, Page(s) 280–292

    Abstract: Aim: To describe and assess the scientific and technical aspects of animal forensic testing at the University of California, Davis. The findings and recommendations contained in this report are designed to assess the past, evaluate the present, and ... ...

    Abstract Aim: To describe and assess the scientific and technical aspects of animal forensic testing at the University of California, Davis. The findings and recommendations contained in this report are designed to assess the past, evaluate the present, and recommend reforms that will assist the animal forensic science community in providing the best possible services that comply with court standards and bear judicial scrutiny.
    Methods: A batch of 32 closed files of domestic dog DNA cases processed at the University of California, Davis, between August 2003 and July 2005 were reviewed in this study. The case files comprised copies of all original paperwork, copies of the cover letter or final report, laboratory notes, notes on analyses, submission forms, internal chains of custody, printed images and photocopies of evidence, as well as the administrative and technical reviews of those cases.
    Results: While the fundamental aspects of animal DNA testing may be reliable and acceptable, the scientific basis for forensic testing animal DNA needs to be improved substantially. In addition to a lack of standardized and validated genetic testing protocols, improvements are needed in a wide range of topics including quality assurance and quality control measures, sample handling, evidence testing, statistical analysis, and reporting.
    Conclusion: This review implies that although a standardized panel of short tandem repeat and mitochondrial DNA markers and publicly accessible genetic databases for canine forensic DNA analysis are already available, the persistent lack of supporting resources, including standardized quality assurance and quality control programs, still plagues the animal forensic community. This report focuses on closed cases from the period 2003-2005, but extends its scope more widely to include other animal DNA forensic testing services.
    MeSH term(s) Animals ; California ; Crime ; DNA/analysis ; DNA, Mitochondrial/analysis ; Databases, Genetic ; Dogs ; Feasibility Studies ; Forensic Genetics/methods ; Genetic Markers ; Microsatellite Repeats/genetics ; Quality Control ; Reproducibility of Results
    Chemical Substances DNA, Mitochondrial ; Genetic Markers ; DNA (9007-49-2)
    Language English
    Publishing date 2011-06-15
    Publishing country Croatia
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Validation Study
    ZDB-ID 1157623-6
    ISSN 1332-8166 ; 0353-9504
    ISSN (online) 1332-8166
    ISSN 0353-9504
    DOI 10.3325/cmj.2011.52.280
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Automation of the standard DNA differential extraction on the Hamilton AutoLys STAR system: A proof-of-concept study.

    Timken, Mark D / Klein, Sonja B / Kubala, Steve / Scharnhorst, Günther / Buoncristiani, Martin R / Miller, Kevin W P

    Forensic science international. Genetics

    2019  Volume 40, Page(s) 96–104

    Abstract: For several decades, a common approach for processing sexual assault evidence has been to use the "standard" differential extraction to separate the evidence into a non-sperm-cell fraction and a sperm-cell fraction for further analysis. In this standard ... ...

    Abstract For several decades, a common approach for processing sexual assault evidence has been to use the "standard" differential extraction to separate the evidence into a non-sperm-cell fraction and a sperm-cell fraction for further analysis. In this standard approach (P. Gill et al., Nature 318 (1985) 577-579), an initial mild chemical lysis step preferentially digests the mainly epithelial cells, which allows for removing this lysate as a non-sperm-cell fraction. The undigested sperm cells in the remaining fraction may then be purified by a series of wash and centrifugation steps, after which more robust lysis conditions are used to digest this sperm-cell fraction. Although this standard approach has been generally effective, it has been difficult to fully automate, due to the variety of different types of manipulations required for sample processing (e.g., incubation, shaking, substrate separation by centrifugation, and multiple liquid transfers for sperm-pellet centrifugation and washing steps). We describe here a fully automated standard differential extraction procedure that uses the Hamilton AutoLys STAR liquid handling assay-ready workstation, which is configured with on-deck components for sample incubation, shaking and centrifugation steps, and works with unique AutoLys-A
    MeSH term(s) Automation ; DNA/isolation & purification ; DNA Fingerprinting ; Female ; Humans ; Male ; Microsatellite Repeats ; Polymerase Chain Reaction ; Robotics ; Semen/chemistry ; Sex Offenses ; Specimen Handling/methods
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2019-02-13
    Publishing country Netherlands
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 2493339-9
    ISSN 1878-0326 ; 1872-4973
    ISSN (online) 1878-0326
    ISSN 1872-4973
    DOI 10.1016/j.fsigen.2019.02.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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