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  1. Book ; Online ; Thesis: Zelluläre Rolle des Valosin-containing Protein (VCP) im kolorektalen Karzinom

    Schmitt, Lisa-Maria [Verfasser] / Buchholz, Malte [Akademischer Betreuer]

    2024  

    Author's details Lisa-Maria Schmitt ; Betreuer: Malte Buchholz
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Philipps-Universität Marburg
    Publishing place Marburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  2. Article ; Online: IL18 Receptor Signaling Inhibits Intratumoral CD8

    Nasiri, Elena / Student, Malte / Roth, Katrin / Siti Utami, Nadya / Huber, Magdalena / Buchholz, Malte / Gress, Thomas M / Bauer, Christian

    Cells

    2023  Volume 12, Issue 3

    Abstract: In pancreatic ductal adenocarcinoma (PDAC), the infiltration of ... ...

    Abstract In pancreatic ductal adenocarcinoma (PDAC), the infiltration of CD8
    MeSH term(s) Animals ; Mice ; Carcinoma, Pancreatic Ductal ; CD8-Positive T-Lymphocytes ; Cell Movement ; Interleukin-18 ; Pancreatic Neoplasms ; Pancreatic Neoplasms
    Chemical Substances Interleukin-18 ; Il18r1 protein, mouse
    Language English
    Publishing date 2023-01-31
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells12030456
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online ; Thesis: Functional roles of the candidate genes COA4 and POLr3K in Pancreatic Ductal Adenocarcinoma

    Yang, Yajing [Verfasser] / Buchholz, Malte [Akademischer Betreuer]

    2023  

    Author's details Yajing Yang ; Betreuer: Malte Buchholz
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Philipps-Universität Marburg
    Publishing place Marburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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    Inter-library loan at ZB MED

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  4. Book ; Online ; Thesis: Phänotypische und molekulare Charakterisierung neuer potenzieller Inhibitoren der ribosomalen Kinase RPS6KA2

    Auer, Nathalie Judith [Verfasser] / Michl, Patrick [Gutachter] / Hüttelmaier, Stefan [Gutachter] / Hämmerle, Monika [Gutachter] / Buchholz, Malte [Gutachter]

    2024  

    Author's details Nathalie Judith Auer ; Gutachter: Patrick Michl, Stefan Hüttelmaier, Monika Hämmerle, Malte Buchholz
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universitäts- und Landesbibliothek Sachsen-Anhalt
    Publishing place Halle (Saale)
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  5. Article ; Online: IL18 Receptor Signaling Inhibits Intratumoral CD8 + T-Cell Migration in a Murine Pancreatic Cancer Model

    Elena Nasiri / Malte Student / Katrin Roth / Nadya Siti Utami / Magdalena Huber / Malte Buchholz / Thomas M. Gress / Christian Bauer

    Cells, Vol 12, Iss 456, p

    2023  Volume 456

    Abstract: In pancreatic ductal adenocarcinoma (PDAC), the infiltration of CD8 + cytotoxic T cells (CTLs) is an important factor in determining prognosis. The migration pattern and interaction behavior of intratumoral CTLs are pivotal to tumor rejection. NLRP3- ... ...

    Abstract In pancreatic ductal adenocarcinoma (PDAC), the infiltration of CD8 + cytotoxic T cells (CTLs) is an important factor in determining prognosis. The migration pattern and interaction behavior of intratumoral CTLs are pivotal to tumor rejection. NLRP3-dependent proinflammatory cytokines IL-1β and IL-18 play a prominent role for CTL induction and differentiation. Here, we investigate the effects of T-cellular IL-1R and IL-18R signaling for intratumoral T-cell motility. Murine adenocarcinoma cell line Panc02 was stably transfected with ovalbumin (OVA) and fluorophore H2B-Cerulean to generate PancOVA H2B-Cerulean tumor cells. Dorsal skinfold chambers (DSFC) were installed on wild-type mice, and PancOVA H2B-Cerulean tumor cells were implanted into the chambers. PancOVA spheroids were formed using the Corning ® Matrigel ® -based 3D cell culture technique. CTLs were generated from OT-1 mice, Il1r −/− OT-1 mice, or Il18r −/− OT-1 mice and were marked with fluorophores. This was followed by the adoptive transfer of CTLs into tumor-bearing mice or the application into tumor spheroids. After visualization with multiphoton microscopy (MPM), Imaris software was used to perform T-cell tracking. Imaris analysis indicates a significantly higher accumulation of Il18r −/− CTLs in PancOVA tumors and a significant reduction in tumor volume compared to wild-type CTLs. Il18r −/− CTLs covered a longer distance (track displacement length) in comparison to wild-type (WT) CTLs, and had a higher average speed (mean track speed). The analysis of instantaneous velocity suggests a higher percentage of arrested tracks (arrests: <4 μm/min) for Il18r −/− CTLs. Our data indicate the contribution of IL-18R signaling to T-cell effector strength, warranting further investigation on phenomena such as intratumoral T-cell exhaustion.
    Keywords live cell imaging ; intravital two-photon microscopy animal models for intravital imaging ; T-cell migration ; tumor spheroid coculture model ; pausing phases/arrest coefficient ; fluorophore ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Book ; Online ; Thesis: Molecular biological analysis of the role of placenta-specific 8 in the context of tumour progression in pancreatic ductal adenocarcinoma

    Stark, Maren [Verfasser] / Buchholz, Malte [Akademischer Betreuer]

    2022  

    Author's details Maren Stark ; Betreuer: Malte Buchholz
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Philipps-Universität Marburg
    Publishing place Marburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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    Inter-library loan at ZB MED

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  7. Article: Corrigendum: Clinical impact of molecular subtyping of pancreatic cancer.

    Zhou, Xu / Hu, Kai / Bailey, Peter / Springfeld, Christoph / Roth, Susanne / Kurilov, Roma / Brors, Benedikt / Gress, Thomas / Buchholz, Malte / An, Jingyu / Wei, Kongyuan / Peccerella, Teresa / Büchler, Markus W / Hackert, Thilo / Neoptolemos, John P

    Frontiers in cell and developmental biology

    2023  Volume 11, Page(s) 1179559

    Abstract: This corrects the article DOI: 10.3389/fcell.2021.743908.]. ...

    Abstract [This corrects the article DOI: 10.3389/fcell.2021.743908.].
    Language English
    Publishing date 2023-03-16
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2023.1179559
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Book ; Online ; Thesis: Auswirkungen des MEN1 Gens auf den Apoptose Signalweg in pankreatischen neuroendokrinen Neoplasien

    Wagener, Nele [Verfasser] / Buchholz, Malte [Akademischer Betreuer]

    2021  

    Author's details Nele Wagener ; Betreuer: Malte Buchholz
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Philipps-Universität Marburg
    Publishing place Marburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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    Inter-library loan at ZB MED

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  9. Book ; Online ; Thesis: Funktionelle Charakterisierung der zellulären Rolle des Kandidatengens Valosin containing protein im Pankreaskarzinom

    Schimanski, Sven [Verfasser] / Buchholz, Malte [Akademischer Betreuer]

    2021  

    Author's details Sven Schimanski ; Betreuer: Malte Buchholz
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Philipps-Universität Marburg
    Publishing place Marburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

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    Inter-library loan at ZB MED

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  10. Article: Toll-like receptor 5 tunes hepatic and pancreatic stellate cells activation.

    Di Fazio, Pietro / Mielke, Sophia / Böhm, Isabell T / Buchholz, Malte / Matrood, Sami / Schuppan, Detlef / Wissniowski, Thaddeus

    BMJ open gastroenterology

    2023  Volume 10, Issue 1

    Abstract: Objective: Stellate cells are responsible for liver and pancreas fibrosis and strictly correlate with tumourigenesis. Although their activation is reversible, an exacerbated signalling triggers chronic fibrosis. Toll-like receptors (TLRs) modulate ... ...

    Abstract Objective: Stellate cells are responsible for liver and pancreas fibrosis and strictly correlate with tumourigenesis. Although their activation is reversible, an exacerbated signalling triggers chronic fibrosis. Toll-like receptors (TLRs) modulate stellate cells transition. TLR5 transduces the signal deriving by the binding to bacterial flagellin from invading mobile bacteria.
    Design: Human hepatic and pancreatic stellate cells were activated by the administration of transforming growth factor-beta (TGF-β). TLR5 was transiently knocked down by short-interference RNA transfection. Reverse Transcription-quantitativePCR and western blot were performed to analyse the transcript and protein level of TLR5 and the transition players. Fluorescence microscopy was performed to identify these targets in spheroids and in the sections of murine fibrotic liver.
    Results: TGF-β-activated human hepatic and pancreatic stellate cells showed an increase of
    Conclusion: TGF-β-mediated activation of hepatic and pancreatic stellate cells requires the over-expression of TLR5. Instead, its autonomous signalling inhibits the activation of the stellate cells, thus prompting a signalling through different regulatory pathways.
    MeSH term(s) Animals ; Humans ; Mice ; Flagellin/pharmacology ; Liver Cirrhosis ; Pancreatic Stellate Cells ; Toll-Like Receptor 5/metabolism
    Chemical Substances Flagellin (12777-81-0) ; Toll-Like Receptor 5 ; TLR5 protein, human ; Tlr5 protein, mouse
    Language English
    Publishing date 2023-07-11
    Publishing country England
    Document type Journal Article
    ISSN 2054-4774
    ISSN 2054-4774
    DOI 10.1136/bmjgast-2023-001148
    Database MEDical Literature Analysis and Retrieval System OnLINE

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