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  1. Article ; Online: Protocol for observing microtubules and microtubule ends in both fixed and live primary microglia cells.

    Sanchini, Caterina / Rosito, Maria / Comincini, Alessandro / De Panfilis, Simone / Bartolini, Francesca / Di Angelantonio, Silvia

    STAR protocols

    2023  Volume 4, Issue 3, Page(s) 102499

    Abstract: Microtubule dynamics and orientation have crucial roles in many vital cellular processes. However, functional live imaging of microtubules and/or microtubule ends in primary microglia can be challenging. Here, we present a protocol for observing ... ...

    Abstract Microtubule dynamics and orientation have crucial roles in many vital cellular processes. However, functional live imaging of microtubules and/or microtubule ends in primary microglia can be challenging. Here, we present a protocol for observing microtubules and microtubule ends in both fixed and live primary microglia cells. We describe steps for microglia culture and in vitro stimulation, SiR-tubulin labeling, lentivirus preparation, live imaging, immunostaining, and image acquisition. We also provide procedures for SiR-tubulin, EB3-EGFP, and EB1 analyses. For complete details on the use and execution of this protocol, please refer to Rosito et al. (2023).
    MeSH term(s) Tubulin/genetics ; Microtubule-Associated Proteins/genetics ; Microglia ; Microtubules
    Chemical Substances Tubulin ; Microtubule-Associated Proteins
    Language English
    Publishing date 2023-08-12
    Publishing country United States
    Document type Journal Article
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2023.102499
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The Central Noradrenergic System in Neurodevelopmental Disorders: Merging Experimental and Clinical Evidence.

    Galgani, Alessandro / Bartolini, Emanuele / D'Amora, Marta / Faraguna, Ugo / Giorgi, Filippo Sean

    International journal of molecular sciences

    2023  Volume 24, Issue 6

    Abstract: The aim of this article is to highlight the potential role of the locus-coeruleus-noradrenergic (LC-NA) system in neurodevelopmental disorders (NdDs). The LC is the main brain noradrenergic nucleus, key in the regulation of arousal, attention, and stress ...

    Abstract The aim of this article is to highlight the potential role of the locus-coeruleus-noradrenergic (LC-NA) system in neurodevelopmental disorders (NdDs). The LC is the main brain noradrenergic nucleus, key in the regulation of arousal, attention, and stress response, and its early maturation and sensitivity to perinatal damage make it an interesting target for translational research. Clinical data shows the involvement of the LC-NA system in several NdDs, suggesting a pathogenetic role in the development of such disorders. In this context, a new neuroimaging tool, LC Magnetic Resonance Imaging (MRI), has been developed to visualize the LC in vivo and assess its integrity, which could be a valuable tool for exploring morphological alterations in NdD in vivo in humans. New animal models may be used to test the contribution of the LC-NA system to the pathogenic pathways of NdD and to evaluate the efficacy of NA-targeting drugs. In this narrative review, we provide an overview of how the LC-NA system may represent a common pathophysiological and pathogenic mechanism in NdD and a reliable target for symptomatic and disease-modifying drugs. Further research is needed to fully understand the interplay between the LC-NA system and NdD.
    MeSH term(s) Animals ; Humans ; Norepinephrine/metabolism ; Locus Coeruleus/metabolism ; Arousal/physiology ; Neurodevelopmental Disorders
    Chemical Substances Norepinephrine (X4W3ENH1CV)
    Language English
    Publishing date 2023-03-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24065805
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  3. Article: Shockwave intravascular lithotripsy in the treatment of under-expanded coronary stent: Case Series.

    Fedele, Massimiliano / Iannone, Alessandro / Bartolini, Davide / Rubartelli, Paolo

    Clinical case reports

    2021  Volume 9, Issue 10, Page(s) e04961

    Abstract: Under-expanded coronary stent related to inadequate preparation of calcified lesion is associated with poor clinical outcomes.Off-label use of S-IVL to correct this clinical issue is effective and safe, probably more than other current techniques. ... ...

    Abstract Under-expanded coronary stent related to inadequate preparation of calcified lesion is associated with poor clinical outcomes.Off-label use of S-IVL to correct this clinical issue is effective and safe, probably more than other current techniques. However, this statement needs further evidence.
    Language English
    Publishing date 2021-10-19
    Publishing country England
    Document type Case Reports
    ZDB-ID 2740234-4
    ISSN 2050-0904
    ISSN 2050-0904
    DOI 10.1002/ccr3.4961
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  4. Article: Impact of carbon pricing on distributed energy systems planning

    Bartolini, Andrea / Mazzoni, Stefano / Comodi, Gabriele / Romagnoli, Alessandro

    Applied energy. 2021 Nov. 01, v. 301

    2021  

    Abstract: Carbon pricing is being implemented by several governments to curb CO₂ emissions. This work studies its impact on distributed energy systems design which are powered by both renewable and fossil fuels. In particular, the analyses investigate a real case ... ...

    Abstract Carbon pricing is being implemented by several governments to curb CO₂ emissions. This work studies its impact on distributed energy systems design which are powered by both renewable and fossil fuels. In particular, the analyses investigate a real case study situated in Singapore, characterized by cooling and electricity demands.The goal of the analyses is to determine whether carbon pricing does impact design choices in meeting the energy demands of a user located in a cooling dominated region, and secondly in assessing the effectiveness of carbon pricing as a CO₂ emissions mitigation policy. This is achieved by investigating the optimal design of the energy systems meeting the demands of the test case under different carbon pricing and primary energy supply costs assumptions.The results indicate that the optimal design under current conditions heavily relies on PV with more than 10 MWp worth of capacity and that an increasing carbon pricing would lead to a lower environmental footprint. But if the supply costs for natural gas were to lower, the optimal design would switch to relying on a combined cooling heat and power-based electricity generation system up to 3 MWe, increasing the primary energy consumptions regardless of the carbon pricing scheme in place. This would also happen even at significantly higher prices than the scheme under evaluation.
    Keywords Singapore ; carbon ; carbon dioxide ; case studies ; ecological footprint ; electricity ; electricity generation ; environmental policy ; heat ; natural gas ; primary energy
    Language English
    Dates of publication 2021-1101
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2000772-3
    ISSN 0306-2619
    ISSN 0306-2619
    DOI 10.1016/j.apenergy.2021.117324
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Lynch Syndrome: From Multidisciplinary Management to Precision Prevention.

    Dal Buono, Arianna / Puccini, Alberto / Franchellucci, Gianluca / Airoldi, Marco / Bartolini, Michela / Bianchi, Paolo / Santoro, Armando / Repici, Alessandro / Hassan, Cesare

    Cancers

    2024  Volume 16, Issue 5

    Abstract: Background and aims: Lynch syndrome (LS) is currently one of the most prevalent hereditary cancer conditions, accounting for 3% of all colorectal cancers and for up to 15% of those with DNA mismatch repair (MMR) deficiency, and it was one of the first ... ...

    Abstract Background and aims: Lynch syndrome (LS) is currently one of the most prevalent hereditary cancer conditions, accounting for 3% of all colorectal cancers and for up to 15% of those with DNA mismatch repair (MMR) deficiency, and it was one of the first historically identified. The understanding of the molecular carcinogenesis of LS tumors has progressed significantly in recent years. We aim to review the most recent advances in LS research and explore genotype-based approaches in surveillance, personalized cancer prevention, and treatment strategies.
    Methods: PubMed was searched to identify relevant studies, conducted up to December 2023, investigating molecular carcinogenesis in LS, surveillance strategies, cancer prevention, and treatment in LS tumors.
    Results: Multigene panel sequencing is becoming the benchmark in the diagnosis of LS, allowing for the detection of a pathogenic constitutional variant in one of the MMR genes. Emerging data from randomized controlled trials suggest possible preventive roles of resistant starch and/or aspirin in LS. Vaccination with immunogenic frameshift peptides appears to be a promising approach for both the treatment and prevention of LS-associated cancers, as evidenced by pre-clinical and preliminary phase 1/2a studies.
    Conclusions: Although robust diagnostic algorithms, including prompt testing of tumor tissue for MMR defects and referral for genetic counselling, currently exist for suspected LS in CRC patients, the indications for LS screening in cancer-free individuals still need to be refined and standardized. Investigation into additional genetic and non-genetic factors that may explain residual rates of interval cancers, even in properly screened populations, would allow for more tailored preventive strategies.
    Language English
    Publishing date 2024-02-20
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers16050849
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  6. Article ; Online: The Central Noradrenergic System in Neurodevelopmental Disorders

    Alessandro Galgani / Emanuele Bartolini / Marta D’Amora / Ugo Faraguna / Filippo Sean Giorgi

    International Journal of Molecular Sciences, Vol 24, Iss 5805, p

    Merging Experimental and Clinical Evidence

    2023  Volume 5805

    Abstract: The aim of this article is to highlight the potential role of the locus-coeruleus–noradrenergic (LC-NA) system in neurodevelopmental disorders (NdDs). The LC is the main brain noradrenergic nucleus, key in the regulation of arousal, attention, and stress ...

    Abstract The aim of this article is to highlight the potential role of the locus-coeruleus–noradrenergic (LC-NA) system in neurodevelopmental disorders (NdDs). The LC is the main brain noradrenergic nucleus, key in the regulation of arousal, attention, and stress response, and its early maturation and sensitivity to perinatal damage make it an interesting target for translational research. Clinical data shows the involvement of the LC-NA system in several NdDs, suggesting a pathogenetic role in the development of such disorders. In this context, a new neuroimaging tool, LC Magnetic Resonance Imaging (MRI), has been developed to visualize the LC in vivo and assess its integrity, which could be a valuable tool for exploring morphological alterations in NdD in vivo in humans. New animal models may be used to test the contribution of the LC-NA system to the pathogenic pathways of NdD and to evaluate the efficacy of NA-targeting drugs. In this narrative review, we provide an overview of how the LC-NA system may represent a common pathophysiological and pathogenic mechanism in NdD and a reliable target for symptomatic and disease-modifying drugs. Further research is needed to fully understand the interplay between the LC-NA system and NdD.
    Keywords locus coeruleus ; noradrenaline ; neurodevelopment ; developmental disorders ; autism ; ADHD ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 610
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: First digital study of the frontal sinus of stem-Canini (Canidae, Carnivora)

    Samuele Frosali / Saverio Bartolini-Lucenti / Joan Madurell-Malapeira / Alessandro Urciuoli / Loïc Costeur / Lorenzo Rook

    Frontiers in Ecology and Evolution, Vol

    evolutionary and ecological insights throughout advanced diagnostic in paleobiology

    2023  Volume 11

    Abstract: IntroductionThe phylogenetic and ecological importance of paranasal sinuses in carnivorans was highlighted by several previous authors, mostly in extant species. Nevertheless, no specific study on this feature on extant canids, and no one on fossil ... ...

    Abstract IntroductionThe phylogenetic and ecological importance of paranasal sinuses in carnivorans was highlighted by several previous authors, mostly in extant species. Nevertheless, no specific study on this feature on extant canids, and no one on fossil representatives of the family, has been published up to now. Here, we analyze for the first time the paranasal sinus of extant and fossil canids through computed tomographic techniques to characterize them morphologically and morphometrically, making ecological inferences.MethodsTo do so, we applied for the first time an innovative deformation-based morphometric approach.ResultsThe results obtained for extant species highlight a remarkable correlation between morphology and ecomorphotypes previously defined by some scholars (namely hypercarnivorous group-hunters; small-prey hypercarnivores, mesocarnivores, hypocarnivores). Our results thus support the direct relationship between diet preferences and the development of frontal sinus in canids. Regarding fossil specimens, we reconstructed for the first time the frontal sinus of three Eucyon species and compared it to those of living forms.DiscussionThe best-preserved specimen, the only known cranium of Eucyon adoxus dated to the Late Pliocene of Saint-Estève (France), displayed similarities with hypercarnivorous group-hunter canids by the large sinus prominences. Given that the overall craniodental morphology of E. adoxus suggests that it acted as a small prey hypercarnivore—similar to extant Canis simensis—the aforementioned affinities might have evolved independently, in relation to high stresses during feeding. Overall, our study demonstrates that morphological inspection and deformation-based geometric morphometrics complement each other and allow a thorough investigation of sinus shape variability, thus enabling the study of sinus morphology in other fossil carnivorans with the ultimate goal of inferring their ecological preferences.
    Keywords innovative morphometrics ; sustainable cultural heritage ; Canidae ; paranasal sinuses ; paleoecology ; Eucyon ; Evolution ; QH359-425 ; Ecology ; QH540-549.5
    Subject code 590
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Microsampling and enantioselective liquid chromatography coupled to mass spectrometry for chiral bioanalysis of novel psychoactive substances.

    Protti, Michele / Varfaj, Ina / Carotti, Andrea / Tedesco, Daniele / Bartolini, Manuela / Favilli, Alessandro / Gerli, Sandro / Mercolini, Laura / Sardella, Roccaldo

    Talanta

    2023  Volume 257, Page(s) 124332

    Abstract: In this paper, the development of efficient enantioselective HPLC methods for the analysis of five benzofuran-substituted phenethylamines, two substituted tryptamines, and three substituted cathinones is described. For the first time, reversed-phase ( ... ...

    Abstract In this paper, the development of efficient enantioselective HPLC methods for the analysis of five benzofuran-substituted phenethylamines, two substituted tryptamines, and three substituted cathinones is described. For the first time, reversed-phase (eluents made up with acidic water-methanol solutions) and polar-ionic (eluent made up with an acetonitrile-methanol solution incorporating both an acidic and a basic additive) conditions fully compatible with mass spectrometry (MS) detectors were applied with a chiral stationary phase (CSP) incorporating the (+)-(18-crown-6)-tetracarboxylic acid chiral selector. Enantioresolution was achieved for nine compounds with α and R
    MeSH term(s) Stereoisomerism ; Methanol ; Tandem Mass Spectrometry/methods ; Chromatography, Liquid/methods ; Chromatography, High Pressure Liquid/methods
    Chemical Substances Methanol (Y4S76JWI15)
    Language English
    Publishing date 2023-02-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2023.124332
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Shockwave intravascular lithotripsy in the treatment of under‐expanded coronary stent

    Massimiliano Fedele / Alessandro Iannone / Davide Bartolini / Paolo Rubartelli

    Clinical Case Reports, Vol 9, Iss 10, Pp n/a-n/a (2021)

    Case Series

    2021  

    Abstract: Abstract Under‐expanded coronary stent related to inadequate preparation of calcified lesion is associated with poor clinical outcomes.Off‐label use of S‐IVL to correct this clinical issue is effective and safe, probably more than other current ... ...

    Abstract Abstract Under‐expanded coronary stent related to inadequate preparation of calcified lesion is associated with poor clinical outcomes.Off‐label use of S‐IVL to correct this clinical issue is effective and safe, probably more than other current techniques. However, this statement needs further evidence.
    Keywords calcified coronary lesion ; coronary lithotripsy ; stent expansion optimization ; Medicine ; R ; Medicine (General) ; R5-920
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Nickel oxide nanoparticles exposure as a risk factor for male infertility: "

    Arato, Iva / Giovagnoli, Stefano / Di Michele, Alessandro / Bellucci, Catia / Lilli, Cinzia / Aglietti, Maria Chiara / Bartolini, Desirée / Gambelunghe, Angela / Muzi, Giacomo / Calvitti, Mario / Eugeni, Elena / Gaggia, Francesco / Baroni, Tiziano / Mancuso, Francesca / Luca, Giovanni

    Frontiers in endocrinology

    2023  Volume 14, Page(s) 1063916

    Abstract: Lately, nickel oxide nanoparticles (NiO NPs) have been employed in different industrial and biomedical fields. Several studies have reported that NiO NPs may affect the development of reproductive organs inducing oxidative stress and, resulting in male ... ...

    Abstract Lately, nickel oxide nanoparticles (NiO NPs) have been employed in different industrial and biomedical fields. Several studies have reported that NiO NPs may affect the development of reproductive organs inducing oxidative stress and, resulting in male infertility. We investigated the
    MeSH term(s) Male ; Animals ; Swine ; Humans ; Reactive Oxygen Species/metabolism ; Sertoli Cells/metabolism ; Nanoparticles ; Risk Factors ; Infertility, Male
    Chemical Substances nickel monoxide (C3574QBZ3Y) ; Reactive Oxygen Species
    Language English
    Publishing date 2023-03-30
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1063916
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