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Article ; Online: A tutorial: Laserspray ionization and related laser-based ionization methods for use in mass spectrometry.

Trimpin, Sarah

2023 Volume 42, Issue 5, Page(s) 2234–2267

Abstract: This Tutorial is to provide a summary of parameters useful for successful outcomes of laserspray ionization (LSI) and related methods that employ a laser to ablate a matrix:analyte sample to produce highly charged ions. In these methods the purpose of ... ...

Abstract	This Tutorial is to provide a summary of parameters useful for successful outcomes of laserspray ionization (LSI) and related methods that employ a laser to ablate a matrix:analyte sample to produce highly charged ions. In these methods the purpose of the laser is to transfer matrix-analyte clusters into the gas phase. Ions are hypothesized to be produced by a thermal process where emitted matrix:analyte gas-phase particles/clusters are charged and loss of matrix from the charged particles leads to release of the analyte ions into the gas phase. The thermal energy responsible for the charge-separation process is relatively low and not necessarily supplied by the laser; a heated inlet tube linking atmospheric pressure with the first vacuum stage of a mass spectrometer is sufficient. The inlet becomes the "ion source", and inter alia, pressure, temperature, and the matrix, which can be a solid, liquid, or combinations, become critical parameters. Injecting matrix:analyte into a heated inlet tube using laser ablation, a shockwave, or simply tapping, all produce the similar mass spectra. Applications are provided that showcase new opportunities in the field of mass spectrometry.
Language	English
Publishing date	2023-07-18
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1491946-1
ISSN	1098-2787 ; 0277-7037
ISSN (online)	1098-2787
ISSN	0277-7037
DOI	10.1002/mas.21762
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Article ; Online: Novel ionization processes for use in mass spectrometry: 'Squeezing' nonvolatile analyte ions from crystals and droplets.

Trimpin, Sarah

Rapid communications in mass spectrometry : RCM

2019 Volume 33 Suppl 3, Page(s) 96–120

Abstract: Together with my group and collaborators, I have been fortunate to have had a key role in the discovery of new ionization processes that we developed into new flexible, sensitive, rapid, reliable, and robust ionization technologies and methods for use in ...

Abstract	Together with my group and collaborators, I have been fortunate to have had a key role in the discovery of new ionization processes that we developed into new flexible, sensitive, rapid, reliable, and robust ionization technologies and methods for use in mass spectrometry (MS). Our current research is focused on how best to understand, improve, and use these novel ionization processes which convert volatile and nonvolatile compounds from solids or liquids into gas-phase ions for analysis by MS using e.g. mass-selected fragmentation and ion mobility spectrometry to provide reproducible, accurate, and improved mass and drift time resolution. In my view, the apex was the discovery of vacuum matrix-assisted ionization (vMAI) in 2012 on an intermediate pressure matrix-assisted laser desorption/ionization (MALDI) source without the use of a laser, high voltages, or any other added energy. Only exposure of the matrix:analyte to the sub-atmospheric pressure of the mass spectrometer was necessary to initiate ionization. These findings were initially rejected by three different scientific journals, with comments related to 'how can this work?', 'where do the charges come from?', and 'it is not analytically useful'. Meanwhile, we and others have demonstrated analytical utility without a complete understanding of the mechanism. In reality, MALDI and electrospray ionization are widely used in science and their mechanisms are still controversially discussed despite use and optimization of now 30 years. This Perspective covers the applications and mechanistic aspects of the novel ionization processes for use in MS that guided us in instrument developments, and provides our perspective on how they relate to traditional ionization processes.
Language	English
Publishing date	2019-06-03
Publishing country	England
Document type	Journal Article
ZDB-ID	58731-x
ISSN	1097-0231 ; 0951-4198
ISSN (online)	1097-0231
ISSN	0951-4198
DOI	10.1002/rcm.8269
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Article ; Online: "Magic" Ionization Mass Spectrometry.

Trimpin, Sarah

Journal of the American Society for Mass Spectrometry

2015 Volume 27, Issue 1, Page(s) 4–21

Abstract: The systematic study of the temperature and pressure dependence of matrix-assisted ionization (MAI) led us to the discovery of the seemingly impossible, initially explained by some reviewers as either sleight of hand or the misinterpretation by an ... ...

Abstract	The systematic study of the temperature and pressure dependence of matrix-assisted ionization (MAI) led us to the discovery of the seemingly impossible, initially explained by some reviewers as either sleight of hand or the misinterpretation by an overzealous young scientist of results reported many years before and having little utility. The “magic” that we were attempting to report was that with matrix assistance, molecules, at least as large as bovine serum albumin (66 kDa), are lifted into the gas phase as multiply charged ions simply by exposure of the matrix:analyte sample to the vacuum of a mass spectrometer. Applied heat, a laser, or voltages are not necessary to achieve charge states and ion abundances only previously observed with electrospray ionization (ESI). The fundamentals of how solid phase volatile or nonvolatile compounds are converted to gas-phase ions without added energy currently involves speculation providing a great opportunity to rethink mechanistic understanding of ionization processes used in mass spectrometry. Improved understanding of the mechanism(s) of these processes and their connection to ESI and matrix-assisted laser desorption/ionization may provide opportunities to further develop new ionization strategies for traditional and yet unforeseen applications of mass spectrometry. This Critical Insights article covers developments leading to the discovery of a seemingly magic ionization process that is simple to use, fast, sensitive, robust, and can be directly applied to surface characterization using portable or high performance mass spectrometers.
MeSH term(s)	Gases/chemistry ; Ions/analysis ; Ions/chemistry ; Pressure ; Proteins ; Spectrometry, Mass, Electrospray Ionization ; Temperature
Chemical Substances	Gases ; Ions ; Proteins
Language	English
Publishing date	2015-10-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1073671-2
ISSN	1879-1123 ; 1044-0305
ISSN (online)	1879-1123
ISSN	1044-0305
DOI	10.1007/s13361-015-1253-4
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Article ; Online: A perspective on MALDI alternatives-total solvent-free analysis and electron transfer dissociation of highly charged ions by laserspray ionization.

Trimpin, Sarah

Journal of mass spectrometry : JMS

2010 Volume 45, Issue 5, Page(s) 471–485

Abstract: Progress in research is hindered by analytical limitations, especially in biological areas in which sensitivity and dynamic range are critical to success. Inherent difficulties of characterization associated with complexity arising from heterogeneity of ... ...

Abstract	Progress in research is hindered by analytical limitations, especially in biological areas in which sensitivity and dynamic range are critical to success. Inherent difficulties of characterization associated with complexity arising from heterogeneity of various materials including topologies (isomeric composition) and insolubility also limit progress. For this reason, we are developing methods for total solvent-free analysis by mass spectrometry consisting of solvent-free ionization followed by solvent-free gas-phase separation. We also recently constructed a novel matrix-assisted laser desorption ionization (MALDI) source that provides a simple, practical and sensitive way of producing highly charged ions by laserspray ionization (LSI) or singly charged ions commonly observed with MALDI by choice of matrix or matrix preparation. This is the first ionization source with such freedom-an extremely powerful analytical 'switch'. Multiply charged LSI ions allow molecules exceeding the mass-to-charge range of the instrument to be observed and permit for the first time electron transfer dissociation fragment ion analysis.
MeSH term(s)	Animals ; Atmospheric Pressure ; Brain Chemistry ; Membrane Proteins/chemistry ; Mice ; Polycyclic Aromatic Hydrocarbons/chemistry ; Polymers/chemistry ; Solvents/chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Stereoisomerism
Chemical Substances	Membrane Proteins ; Polycyclic Aromatic Hydrocarbons ; Polymers ; Solvents
Language	English
Publishing date	2010-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ZDB-ID	1221763-3
ISSN	1096-9888 ; 1076-5174
ISSN (online)	1096-9888
ISSN	1076-5174
DOI	10.1002/jms.1737
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Zs.MO 220: Show issues

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Article ; Online: Matrix-assisted ionization mass spectrometry in targeted protein analysis - An initial evaluation.

Skjaervø, Øystein / Trimpin, Sarah / Halvorsen, Trine Grønhaug

Rapid communications in mass spectrometry : RCM

2019 Volume 35 Suppl 1, Page(s) e8437

Abstract: Rationale: Matrix-assisted ionization (MAI) is a relatively new ionization technique for analysis by mass spectrometry (MS). The technique is simple and has been shown to be less influenced by matrix effects than e.g. electrospray ionization (ESI). ... ...

Abstract	Rationale: Matrix-assisted ionization (MAI) is a relatively new ionization technique for analysis by mass spectrometry (MS). The technique is simple and has been shown to be less influenced by matrix effects than e.g. electrospray ionization (ESI). These features are of interest in the targeted analysis of proteins from biological samples. Methods: Targeted protein determination by MAI-MS was evaluated using a triple quadrupole mass analyzer equipped with a stripped nanoESI source in selected reaction monitoring (SRM) mode. The proteins were analyzed using the bottom-up approach with stable isotopic labeled peptides as internal standards (IS). The MAI matrix was 3-nitrobenzonitrile dissolved in acetonitrile. Aqueous sample and matrix solution were mixed in a 1:3 volume ratio. One microlitre of the dried matrix/analyte sample was introduced into the inlet of the mass spectrometer where ionization commences. Results: SRM settings established for ESI-SRM-MS of the peptides here investigated were applicable in MAI-SRM-MS for all evaluated peptides except one that is poorly soluble in water. Addition of IS provided efficient correction at most levels (relative standard deviation (RSD) ≤28% (except lowest digest level), r Conclusions: The results demonstrate that MAI-SRM-MS has promising potential in targeted protein determination by the bottom-up approach because of its simplicity, ease of use, and speed. However, more data is needed to confirm the results prior to application in a clinical setting.
MeSH term(s)	Humans ; Isotope Labeling/methods ; Linear Models ; Peptides/blood ; Peptides/chemistry ; Peptides/urine ; Proteins/analysis ; Proteins/chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Chemical Substances	Peptides ; Proteins
Language	English
Publishing date	2019-05-23
Publishing country	England
Document type	Journal Article
ZDB-ID	58731-x
ISSN	1097-0231 ; 0951-4198
ISSN (online)	1097-0231
ISSN	0951-4198
DOI	10.1002/rcm.8437
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Article ; Online: A Combination MAI and MALDI Vacuum Source Operational from Atmospheric Pressure for Fast, Robust, and Sensitive Analyses.

Hoang, Khoa / Trimpin, Sarah / McEwen, Charles N / Pophristic, Milan

Journal of the American Society for Mass Spectrometry

2020 Volume 32, Issue 1, Page(s) 124–132

Abstract: Previously, vacuum matrix-assisted ionization (vMAI) was employed with matrix/analyte sample introduction into the vacuum of a mass spectrometer on a probe sample introduction device. Low attomole detection was achieved, while no carryover was observed ... ...

Abstract	Previously, vacuum matrix-assisted ionization (vMAI) was employed with matrix/analyte sample introduction into the vacuum of a mass spectrometer on a probe sample introduction device. Low attomole detection was achieved, while no carryover was observed even for concentrated samples. Here, we report a new vacuum ionization source designed to duplicate the sensitivity and robustness of probe device while providing fast multisample introduction to vacuum and rapid sequential ionization. Exposure of a sample to the vacuum of the mass spectrometer provides spontaneous ionization of volatile as well as nonvolatile analytes without the need for external energy input. However, the novel source design described herein, in addition to vMAI, can employ a laser to obtain vacuum matrix-assisted laser desorption/ionization (vMALDI). In particular, ionization by vMAI or vMALDI is achieved by using the appropriate matrix. Switching between ionization modes is accomplished in a few seconds. We present results demonstrating the utility of the two ionization methods in combination to improve the molecular analyses of sample composition. In both ionization modes, multiple samples can be sequentially and rapidly acquired to increase throughput in MS. With the prototype source, samples were acquired in as little as 1 s per sample. Exchanging multisample plates can be accomplished in as little as 2 s, suggesting low-cost high-throughput automation when properly developed.
Language	English
Publishing date	2020-12-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	1073671-2
ISSN	1879-1123 ; 1044-0305
ISSN (online)	1879-1123
ISSN	1044-0305
DOI	10.1021/jasms.0c00298
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Zs.MO 241: Show issues

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Article ; Online: Advances in Ionization for Mass Spectrometry.

Peacock, Patricia M / Zhang, Wen-Jing / Trimpin, Sarah

Analytical chemistry

2017 Volume 89, Issue 1, Page(s) 372–388

Language	English
Publishing date	2017--03
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.6b04348
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Article ; Online: Instrumentation development, improvement, simplification, and miniaturization: The multifunctional plate source for use in mass spectrometry.

Trimpin, Sarah / Inutan, Ellen / Coffinberger, Hope / Hoang, Khoa / Yenchick, Frank / Wager-Miller, James / Pophristic, Milan / Mackie, Ken / McEwen, Charles N

European journal of mass spectrometry (Chichester, England)

2023 Volume 29, Issue 5-6, Page(s) 276–291

Abstract: In remembrance of Prof. Dr Przybylski, we are presenting a vision towards his beloved mass spectrometry (MS) and its far-reaching promises outside of the academic laboratory. Sub-atmospheric pressure (AP) ionization MS is well positioned to make a step- ... ...

Abstract	In remembrance of Prof. Dr Przybylski, we are presenting a vision towards his beloved mass spectrometry (MS) and its far-reaching promises outside of the academic laboratory. Sub-atmospheric pressure (AP) ionization MS is well positioned to make a step-change in direct ionization, a concept that allows
Language	English
Publishing date	2023-11-24
Publishing country	England
Document type	Journal Article
ISSN	1751-6838
ISSN (online)	1751-6838
DOI	10.1177/14690667231211486
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Article ; Online: High-throughput solvent assisted ionization inlet for use in mass spectrometry.

Wang, Beixi / Trimpin, Sarah

Analytical chemistry

2014 Volume 86, Issue 2, Page(s) 1000–1006

Abstract: In this work we developed a multiplexed analysis platform providing a simple high-throughput means to characterize solutions. Automated analyses, requiring less than 5 s per sample without carryover and 1 s per sample, accepting minor cross contamination, ...

Abstract	In this work we developed a multiplexed analysis platform providing a simple high-throughput means to characterize solutions. Automated analyses, requiring less than 5 s per sample without carryover and 1 s per sample, accepting minor cross contamination, was achieved using multiplexed solvent assisted ionization inlet (SAII) mass spectrometry (MS). The method involves sequentially moving rows of pipet tips containing sample solutions in close proximity to the inlet aperture of a heated mass spectrometer inlet tube. The solution is pulled from the container into the mass spectrometer inlet by the pressure differential at the mass spectrometer inlet aperture. This sample introduction method for direct injection of solutions is fast, easily implemented, and widely applicable, as is shown by applications ranging from small molecules to proteins as large as carbonic anhydrase (molecular weight ca. 29,000). MS/MS fragmentation is applicable for sample characterization. An x,y-stage and common imaging software are incorporated to map the location of components in the sample wells of a microtiter plate. Location within an x,y-array of different sample solutions and the relative concentration of the sample are displayed using ion intensity maps.
MeSH term(s)	Angiotensin II/analysis ; Animals ; Calcitonin/analysis ; Carbonic Anhydrases/analysis ; Cattle ; Cholecystokinin/analysis ; Humans ; Molecular Weight ; Phosphoproteins/analysis ; Pressure ; Serum Albumin, Bovine/analysis ; Solvents ; Spectrometry, Mass, Electrospray Ionization/instrumentation ; Spectrometry, Mass, Electrospray Ionization/methods ; Tandem Mass Spectrometry/instrumentation ; Tandem Mass Spectrometry/methods ; Ubiquitin/analysis
Chemical Substances	Phosphoproteins ; Solvents ; Ubiquitin ; Angiotensin II (11128-99-7) ; Serum Albumin, Bovine (27432CM55Q) ; Calcitonin (9007-12-9) ; Cholecystokinin (9011-97-6) ; Carbonic Anhydrases (EC 4.2.1.1)
Language	English
Publishing date	2014-01-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/ac400867b
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Article: A perspective on MALDI alternatives--total solvent-free analysis and electron transfer dissociation of highly charged ions by laserspray ionization

Trimpin, Sarah

Journal of mass spectrometry. 2010 May, v. 45, no. 5

2010

Abstract	Progress in research is hindered by analytical limitations, especially in biological areas in which sensitivity and dynamic range are critical to success. Inherent difficulties of characterization associated with complexity arising from heterogeneity of various materials including topologies (isomeric composition) and insolubility also limit progress. For this reason, we are developing methods for total solvent-free analysis by mass spectrometry consisting of solvent-free ionization followed by solvent-free gas-phase separation. We also recently constructed a novel matrix-assisted laser desorption ionization (MALDI) source that provides a simple, practical and sensitive way of producing highly charged ions by laserspray ionization (LSI) or singly charged ions commonly observed with MALDI by choice of matrix or matrix preparation. This is the first ionization source with such freedom--an extremely powerful analytical 'switch'. Multiply charged LSI ions allow molecules exceeding the mass-to-charge range of the instrument to be observed and permit for the first time electron transfer dissociation fragment ion analysis. Copyright © 2010 John Wiley & Sons, Ltd.
Keywords	desorption ; dissociation ; electron transfer ; ionization ; ions ; mass spectrometry ; materials
Language	English
Dates of publication	2010-05
Size	p. 471-485.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article
ZDB-ID	1221763-3
ISSN	1096-9888 ; 1076-5174
ISSN (online)	1096-9888
ISSN	1076-5174
DOI	10.1002/jms.1737
Database	NAL-Catalogue (AGRICOLA)

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Zs.MO 220: Show issues

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