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  1. Article ; Online: Echinococcus granulosus: Insights into the protoscolex F-actin cytoskeleton.

    La-Rocca, Silvana / Farias, Joaquina / Chalar, Cora / Kun, Alejandra E / Fernandez, Veronica

    Acta tropica

    2019  Volume 199, Page(s) 105122

    Abstract: ... develop inside its hosts, completing thus its life cycle. We focused our attention on F-actin organization ... of the parasite cytoskeleton. In particular, we addressed some aspects of F-actin rearrangements in PSC ... different PSC structures and phalloidin staining displayed a parasite's highly organized F ...

    Abstract Echinococcus granulosus is a cestode parasite whose cytoskeleton plasticity allows it to enter and develop inside its hosts, completing thus its life cycle. We focused our attention on F-actin organization and distribution in E. granulosus protoscoleces (PSC) in order to contribute to the knowledge of the parasite cytoskeleton. In particular, we addressed some aspects of F-actin rearrangements in PSC at different stages of the evagination/invagination process. The use of light microscopy allowed us to identify different PSC structures and phalloidin staining displayed a parasite's highly organized F-actin cytoskeleton. Suckers exhibit an important musculature composed of a set of radial fibers. At the rostellum, the F-actin filaments are arranged in a bulbar shape with perforations that appear to be the attachment places for the hooks. Also, "circular" structures of F-actin were identified, which remind the flame cells. Furthermore, parasite F-actin filaments, unevenly distributed, seem to have remained substantially unchanged during the evagination/invagination process. Finally, we showed that the scolex of an evaginated E. granulosus PSC reinvaginates in vitro without any treatment.
    MeSH term(s) Abattoirs ; Actin Cytoskeleton/physiology ; Actin Cytoskeleton/ultrastructure ; Actins/analysis ; Animals ; Cattle ; Echinococcus granulosus/anatomy & histology ; Echinococcus granulosus/growth & development ; Echinococcus granulosus/physiology ; Life Cycle Stages ; Lung/parasitology ; Microscopy, Fluorescence ; Phalloidine ; Staining and Labeling ; Uruguay
    Chemical Substances Actins ; Phalloidine (17466-45-4)
    Language English
    Publishing date 2019-08-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2019.105122
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.136: Show issues Location:
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  2. Article ; Online: Chemical characterization, in vitro anticancer effect of Asplenium aethiopicum (Burm. f.) Becherer

    alias Antonysamy, Johnson Marimuthu / Janarthanan, Gowtham / Rocha, Janaina Esmeraldo / de Almeida, Ray Silva / de Vasconcelos, John Eversong Lucena / dos Santos, Francisco Antonio Vieira / de Farias, Pablo Antonio Maia / Coutinho, Henrique D.M.

    SAAB South African Journal of Botany. 2023 Mar., v. 154 p.372-379

    2023  

    Abstract: ... as medicinal plants. Previous studies on Asplenium aethiopicum (Burm. f.) Becherer determined the antioxidant ... to reveal the chemical constituents and anticancer properties of Asplenium aethiopicum (Burm. f.) Becherer ...

    Abstract Human interests for ferns and fern allies will be not only ornamental but also it can be used as medicinal plants. Previous studies on Asplenium aethiopicum (Burm. f.) Becherer determined the antioxidant, larvicidal and cytotoxic potential using brine shrimp biolethality assay. The present study was intended to reveal the chemical constituents and anticancer properties of Asplenium aethiopicum (Burm. f.) Becherer using A549 (lung adenocarcinoma) and African Green Monkey Kidney cell lines. The preliminary phytochemical, UV–Vis, FT-IR analysis were performed using various extracts of A. aethiopicum. The GC-MS and anticancer activity was performed in the ethanolic extracts of A. aethiopicum. Anticancer potential of A. aethiopicum ethanolic extracts on both A549 (lung adenocarcinoma) and African Green Monkey Kidney cells was determined by the 3-[4,5-ethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide assay. A. aethiopicum acetone, ethanolic and aqueous extracts were showed the presence of five metabolites. FT-IR analysis confirmed the presence of various functional groups in the A. aethiopicum ethanolic extracts. GC-MS analysis showed the presence of two major components viz., Bicyclo [4.1.0] heptan-2-one (13.92%) and Tridecane (10.50%). The A. aethiopicum ethanolic extracts showed the anticancer activity against human A549 lung adenocarcinoma in a dose and time-dependent inhibitory effect. Anticancer activity of A. aethiopicum ethanolic extracts may be due to the presence of bioactive compounds viz., Bicyclo[4.1.0]heptan-2-one,Cyclohexanaone,5-ethyl-4-(1-methylethenyl)-2-(1methylethylidene), cis-, 5,8,11-Eicosatriyonic acid, methyl ester, 1,5,5-Trimethyl-6-methylene-cyclohexane in the ethanolic extracts of A. aethiopicum. Further studies are needed to isolate the active principle responsible for anticancer activity.
    Keywords Artemia ; Asplenium ; Cercopithecus aethiops ; acetone ; adenocarcinoma ; antineoplastic activity ; antioxidants ; cytotoxicity ; ferns and fern allies ; humans ; kidneys ; larvicides ; lungs ; metabolites ; phytochemicals ; Anticancer ; GC-MS ; Phytoconstituents ; FT-IR ; Asplenium aethiopicum
    Language English
    Dates of publication 2023-03
    Size p. 372-379.
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 2126918-X
    ISSN 0254-6299
    ISSN 0254-6299
    DOI 10.1016/j.sajb.2023.01.035
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  3. Article ; Online: Superação de dormência em sementes da Cassia grandis L. f. (Fabaceae)

    Ednaldo Vieira do Nascimento / Oriel Herrera Bonilla / Eliseu Marlônio Pereira de Lucena / Sandro Ferreira do Nascimento / Ivina Beatriz Menezes Farias / Letícia Holanda de Sousa

    Revista Verde de Agroecologia e Desenvolvimento Sustentável , Vol 16, Iss

    2021  Volume 1

    Abstract: ... é um processo de distribuição da germinação das sementes no tempo e no espaço. A Cassia grandis L. f., espécie ...

    Abstract Estudos sobre tecnologia de sementes fundamentam-se na análise de temperaturas e métodos pré-germinativos; é preciso aprimorar os que se mostram mais eficientes, otimizando tempo e recursos. A dormência é um processo de distribuição da germinação das sementes no tempo e no espaço. A Cassia grandis L. f., espécie arbórea, pertencente à família Fabaceae, possui importância ambiental e socioeconômica. O objetivo deste trabalho foi superar a dormência de sementes de C. grandis, através de diferentes métodos. As sementes foram submetidas aos tratamentos de escarificação mecânica, química e testemunha; germinadas nas temperaturas de 25 e de 30 °C. O delineamento experimental adotado, foi do tipo inteiramente casualizado, com quatro repetições, fatorial 3x2 e 24 parcelas. Avaliou-se: porcentagem e índice de velocidade de germinação, comprimento e massa fresca e seca de radícula, hipocótilo e plântula. As sementes de C. grandis possuem dormência física, sendo os métodos mais eficientes de superação a escarificação mecânica, com lixa para madeira número 100, na região oposta à micrópila; e a escarificação química, com ácido sulfúrico concentrado, com tempo de duração de 30 min. de imersão, independente da temperatura testada.
    Keywords Germinação ; Semente florestal ; Espécie melífera ; Restauração florestal ; Agriculture (General) ; S1-972 ; Geography. Anthropology. Recreation ; G ; Environmental sciences ; GE1-350
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Grupo Verde de Agroecologia e Abelhas (GVAA)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Evaluation of sensitization to the crude extract of Dermatophagoides farinae and its derived allergens, Der f 2 and Zen 1, in dogs with atopic dermatitis in Southern Brazil.

    Ludwig, L / Tsukui, T / Kageyama, M / Farias, M

    Veterinary immunology and immunopathology

    2021  Volume 234, Page(s) 110199

    Abstract: ... in the disease.: Objectives: We aimed to observe the allergen-specific IgE against crudeD. farinae, Der f 2 ... positivity against crudeD. farinae (92 %) and Zen 1 (77 %) was higher than Der f 2 (56 %). There was ... Der f 2 and Der f 2 and Zen 1. The sensitization to D. farinae and its allergens was associated ...

    Abstract Background: Atopic dermatitis is associated with the production of IgE antibodies against environmental allergens and allergens of the house dust miteDermatophagoides farinae are frequently implicated in the disease.
    Objectives: We aimed to observe the allergen-specific IgE against crudeD. farinae, Der f 2 and Zen 1 in dogs with atopic dermatitis and report if these dogs are in contact with material that could shelter mite allergens.
    Methods: 100 dogs with clinical diagnosis of atopic dermatitis were included after exclusion of other forms of pruritic skin disease and dogs that already received specific or non-specific immunotherapy. These dogs were of different breeds and ages and they were presented at a veterinary teaching hospital and a private service of veterinary dermatology, both located in Curitiba, Southern Brazil. At the time of anamnesis, some questions were applied to know the possibility of these dogs having had contact with furniture and textile material which could shelter house dust mites. Sera samples were obtained and further analyzed by ELISA assay to measure serum IgE levels against these allergens with an established cut-off of 0.200 IgE optical density.
    Results: The allergen-specific IgE positivity against crudeD. farinae (92 %) and Zen 1 (77 %) was higher than Der f 2 (56 %). There was a correlation in sensitization to crude D. farinae and Zen 1 that was not observed between crude D. farinae and Der f 2 and Der f 2 and Zen 1. The sensitization to D. farinae and its allergens was associated with an unrestricted exposition to furniture and textile material. CONCLUSION & CLINICAL RELEVANCE: dogs with atopic dermatitis are frequently sensitized to D. farinae and its allergens, Der f 2 and Zen 1, may be considered major allergens in these dogs. Zen 1 may be the main allergen responsible for the sensitization to crude D. farinae.
    MeSH term(s) Allergens/administration & dosage ; Allergens/classification ; Allergens/immunology ; Animals ; Antigens, Dermatophagoides/administration & dosage ; Antigens, Dermatophagoides/immunology ; Arthropod Proteins/administration & dosage ; Arthropod Proteins/immunology ; Brazil ; Complex Mixtures/administration & dosage ; Complex Mixtures/immunology ; Dermatitis, Atopic/immunology ; Dermatitis, Atopic/veterinary ; Dermatophagoides farinae/chemistry ; Dermatophagoides farinae/immunology ; Dog Diseases/immunology ; Dogs ; Female ; Hospitals, Animal ; Immunization/methods ; Immunization/standards ; Immunoglobulin E/blood ; Male
    Chemical Substances Allergens ; Antigens, Dermatophagoides ; Arthropod Proteins ; Complex Mixtures ; Dermatophagoides farinae antigen f 2 ; Immunoglobulin E (37341-29-0)
    Language English
    Publishing date 2021-02-10
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 754160-0
    ISSN 1873-2534 ; 0165-2427
    ISSN (online) 1873-2534
    ISSN 0165-2427
    DOI 10.1016/j.vetimm.2021.110199
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2177: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article: Echinococcus granulosus: Insights into the protoscolex F-actin cytoskeleton

    La-Rocca, Silvana / Farias, Joaquina / Chalar, Cora / Kun, Alejandra E / Fernandez, Veronica

    Acta tropica. 2019 Aug. 01,

    2019  

    Abstract: ... develop inside its hosts, completing thus its life cycle. We focused our attention on F-actin organization ... of the parasite cytoskeleton. In particular, we addressed some aspects of F-actin rearrangements in PSC ... different PSC structures and phalloidin staining displayed a parasite's highly organized F ...

    Abstract Echinococcus granulosus is a cestode parasite whose cytoskeleton plasticity allows it to enter and develop inside its hosts, completing thus its life cycle. We focused our attention on F-actin organization and distribution in E. granulosus protoscoleces (PSC) in order to contribute to the knowledge of the parasite cytoskeleton. In particular, we addressed some aspects of F-actin rearrangements in PSC at different stages of the evagination/invagination process. The use of light microscopy allowed us to identify different PSC structures and phalloidin staining displayed a parasite's highly organized F-actin cytoskeleton. Suckers exhibit an important musculature composed of a set of radial fibers. At the rostellum, the F-actin filaments are arranged in a bulbar shape with perforations that appear to be the attachment places for the hooks. Also, "circular" structures of F-actin were identified, which remind the flame cells. Furthermore, parasite F-actin filaments, unevenly distributed, seem to have remained substantially unchanged during the evagination/invagination process. Finally, we showed that the scolex of an evaginated E. granulosus PSC reinvaginates in vitro without any treatment.
    Keywords Echinococcus granulosus ; actin ; cytoskeleton ; hosts ; light microscopy ; parasites ; protoscoleces ; staining
    Language English
    Dates of publication 2019-0801
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2019.105122
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    Z 52/75: Show issues

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  6. Article ; Online: Effect of Ylang-Ylang ( Cananga odorata Hook. F. & Thomson) Essential Oil on Acute Inflammatory Response In Vitro and In Vivo

    Robson Araújo de Freitas Junior / Paloma Kênia de Moraes Berenguel Lossavaro / Cândida Aparecida Leite Kassuya / Edgar Julian Paredes-Gamero / Nelson Carvalho Farias Júnior / Maria Inês Lenz Souza / Francielli Maria de Souza Silva-Comar / Roberto Kenji Nakamura Cuman / Denise Brentan Silva / Mônica Cristina Toffoli-Kadri / Saulo Euclides Silva-Filho

    Molecules, Vol 27, Iss 3666, p

    2022  Volume 3666

    Abstract: ... of ylang-ylang ( Cananga odorata Hook. F. & Thomson) essential oil (YEO) on acute inflammation. YEO was analyzed ...

    Abstract The aim of this study is to evaluate the phytochemical profile, oral acute toxicity, and the effect of ylang-ylang ( Cananga odorata Hook. F. & Thomson) essential oil (YEO) on acute inflammation. YEO was analyzed by gas chromatography/mass spectrometry. For in vitro tests, YEO was assessed using cytotoxicity, neutrophil chemotaxis induced by N -formyl methionyl leucyl phenylalanine (fMLP), and phagocytic activity tests. YEO was orally administered in zymosan-induced peritonitis, carrageenan-induced leukocyte rolling, and adhesion events in the in situ microcirculation model and in carrageenan-induced paw edema models. YEO (2000 mg/kg) was also tested using an acute toxicity test in Swiss mice. YEO showed a predominance of benzyl acetate, linalool, benzyl benzoate, and methyl benzoate. YEO did not present in vitro cytotoxicity. YEO reduced the in vitro neutrophil chemotaxis induced by fMLP and reduced the phagocytic activity. The oral treatment with YEO reduced the leukocyte recruitment and nitric oxide production in the zymosan-induced peritonitis model, reduced rolling and adherent leukocyte number induced by carrageenan in the in situ microcirculation model, and reduced carrageenan-induced edema and mechanical hyperalgesia. YEO did not present signs of toxicity in the acute toxicity test. In conclusion, YEO affected the leukocyte activation, and presented antiedematogenic, anti-hyperalgesic, and anti-inflammatory properties.
    Keywords ylang-ylang ; essential oil ; inflammatory response ; natural products ; Organic chemistry ; QD241-441
    Subject code 570
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Activity of physalin F in a collagen-induced arthritis model.

    Brustolim, Daniele / Vasconcelos, Juliana F / Freitas, Luiz Antônio R / Teixeira, Mauro M / Farias, Marcel T / Ribeiro, Yvone M / Tomassini, Therezinha C B / Oliveira, Geraldo G S / Pontes-de-Carvalho, Lain C / Ribeiro-dos-Santos, Ricardo / Soares, Milena B P

    Journal of natural products

    2010  Volume 73, Issue 8, Page(s) 1323–1326

    Abstract: The effects of physalin F (1), a steroid derivative purified from Physalis angulata, were ... of the immunosuppressive activity of physalin F is distinct from that of the glucocorticoids. ...

    Abstract The effects of physalin F (1), a steroid derivative purified from Physalis angulata, were investigated in models of collagen-induced arthritis in DBA/1 mice and allergic airway inflammation in BALB/c mice. Oral treatment with 1 or dexamethasone caused a marked decrease in paw edema and joint inflammation when compared to vehicle-treated arthritic mice. In contrast, treatment with 1 had no effect in mice with allergic airway inflammation caused by ovalbumin immunization, whereas dexamethasone significantly reduced the number of inflammatory cells and eosinophils in the broncoalveolar lavage fluid and in lung sections of challenged mice. To further demonstrate that 1 acts through a mechanism different from that of glucocorticoids, a nuclear translocation assay was performed of the glucocorticoid receptor (GR) using COS-7 cells transfected with a plasmid encoding for a yellow fluorescent protein (YFP)-GR fusion protein. Untreated or treated cells with 1 had YFP staining mainly in the cytoplasm, whereas in dexamethasone-treated cells the YFP staining was concentrated in the nuclei. It is concluded that the mechanism of the immunosuppressive activity of physalin F is distinct from that of the glucocorticoids.
    MeSH term(s) Administration, Oral ; Animals ; Anti-Inflammatory Agents/chemistry ; Anti-Inflammatory Agents/pharmacology ; Arthritis, Experimental ; COS Cells ; Cercopithecus aethiops ; Dexamethasone/pharmacology ; Disease Models, Animal ; Eosinophils/drug effects ; Glucocorticoids/adverse effects ; Glucocorticoids/immunology ; Inflammation/chemically induced ; Mice ; Mice, Inbred BALB C ; Molecular Structure ; Ovalbumin/administration & dosage ; Physalis/chemistry ; Secosteroids/chemistry ; Secosteroids/immunology ; Secosteroids/pharmacology
    Chemical Substances Anti-Inflammatory Agents ; Glucocorticoids ; Secosteroids ; physalin F (57423-71-9) ; Dexamethasone (7S5I7G3JQL) ; Ovalbumin (9006-59-1)
    Language English
    Publishing date 2010-08-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 304325-3
    ISSN 1520-6025 ; 0163-3864
    ISSN (online) 1520-6025
    ISSN 0163-3864
    DOI 10.1021/np900691w
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1144: Show issues Location:
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  8. Article ; Online: The essential oil of the leaves of Verbesina macrophylla (Cass.) S.F.Blake has antimicrobial, anti-inflammatory and antipyretic activities and is toxicologically safe.

    de Veras, Bruno Oliveira / de Oliveira, João Ricardhis Saturnino / de Menezes Lima, Vera Lúcia / do Amaral Ferraz Navarro, Daniela Maria / de Oliveira Farias de Aguiar, Júlio César Ribeiro / de Medeiros Moura, Geovanna Maria / da Silva, José Wellinton / de Assis, Caio Rodrigo Dias / Gorlach-Lira, Krystyna / de Assis, Priscilla Anne Castro / de Souza Barbosa, Jorge Irapuan / de Melo, Maria Rita Cabral Sales / de Oliveira, Maria Betânia Melo / da Silva, Márcia Vanusa / de Souza Lopes, Ana Catarina

    Journal of ethnopharmacology

    2020  Volume 265, Page(s) 113248

    Abstract: Ethnopharmacological relevance: Verbesina macrophylla (Cass.) S.F.Blake is a medicinal plant ... validates the main activities attributed to the traditional use of Verbesina macrophylla (Cass.) S.F.Blake ...

    Abstract Ethnopharmacological relevance: Verbesina macrophylla (Cass.) S.F.Blake is a medicinal plant from South America, popularly known as "asa de peixe", "asa de peixe branco", "cambará branco" or "cambará guaçu", being used by traditional communities for its healing powers in the form of teas, infusions, liqueurs and extracts, for the treatment of bacterial and fungal infections of the urinary and respiratory tracts, such as kidney problems, bronchitis, inflammation and fever. However, none of the ethnopharmacological properties has been scientifically evaluated.
    Aim of the study: Based on the ethnopharmacological use of the species, this study investigated the chemical composition, and for the first time acute toxicity, hemolytic, antimicrobial, anti-inflammatory and antipyretic activities of the essential oil from leaves of V. macrophylla.
    Material and methods: The essential oil was obtained from the leaves by hydrodistillation (HD), being characterized by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography coupled to flame ionization detection (GC-FID). The antimicrobial activity was evaluated by the broth microdilution technique in bacteria and fungi that cause infections of the respiratory and urinary tract, and toxicological safety regarding hemolytic activity on human red blood cells (hRBCs), and acute toxicity in mice. The anti-inflammatory activity was evaluated by the model carrageenan-induced peritonitis with quantification of the levels of TNF-α and IL-1β in the intraperitoneal fluid, and ear edema induced by croton oil. The antipyretic activity evaluated in mice with pyrexia induced by yeast.
    Results: The extraction of essential oil by hydrodistillation (HD) showed a yield of 0.33 ± 0.04%, with its composition constituted mainly by sesquiterpenes of hydrocarbons (94.00%). The essential oil demonstrated antibacterial and antifungal activity, with a low rate of hemolysis in human red blood cells (hRBCs) and no clinical signs of toxicity were observed in animals after acute treatment, which suggested that the LD
    Conclusion: The set of results obtained, validates the main activities attributed to the traditional use of Verbesina macrophylla (Cass.) S.F.Blake. These data add industrial value to the species, considering that the antimicrobial, anti-inflammatory and antipyretic activities present results similar to the drugs already used also presenting safety. The results suggest that essential oil from V. macrophylla may be used by industry for the development of drugs with natural antimicrobial, anti-inflammatory and antipyretic effect.
    MeSH term(s) Animals ; Anti-Infective Agents/isolation & purification ; Anti-Infective Agents/pharmacology ; Anti-Inflammatory Agents/isolation & purification ; Anti-Inflammatory Agents/pharmacology ; Antipyretics/isolation & purification ; Antipyretics/pharmacology ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Erythrocytes/drug effects ; Female ; Fever/drug therapy ; Hemolysis/drug effects ; Humans ; Inflammation/drug therapy ; Male ; Mice ; Oils, Volatile/isolation & purification ; Oils, Volatile/pharmacology ; Oils, Volatile/toxicity ; Plant Extracts/pharmacology ; Plant Extracts/toxicity ; Plant Leaves ; Toxicity Tests, Acute ; Verbesina/chemistry
    Chemical Substances Anti-Infective Agents ; Anti-Inflammatory Agents ; Antipyretics ; Oils, Volatile ; Plant Extracts
    Language English
    Publishing date 2020-08-14
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2020.113248
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1494: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  9. Article ; Online: Atividades biológicas de plantas da família Bromeliaceae e da espécie Encholirium spectabile Mart. ex Schult. & Schult. f.

    Jorge Andrés García Suárez / Rodrigo José Nunes Calumby / Fabianny Torres de Oliveira / Daniglayse Santos Vieira / Jayane Omena de Oliveira / Rossana Teotônio de Farias Moreira / Maria Lysete de Assis Bastos / Regina Célia Sales Santos Veríssimo

    Research, Society and Development, Vol 9, Iss 12, Pp e33091211019-e

    2020  Volume 33091211019

    Abstract: ... spectabile Mart. ex Schult. & Schult. f. Trata-se de uma revisão integrativa da literatura nas bases de dados ...

    Abstract Bromeliaceae compreende um numeroso grupo de espécies vegetais possuidoras de um vasto potencial biológico, ainda pouco estudado. Diante disto, este estudo teve por objetivo identificar as evidências científicas acerca do potencial biológico de plantas da família Bromeliaceae e da espécie Encholirium spectabile Mart. ex Schult. & Schult. f. Trata-se de uma revisão integrativa da literatura nas bases de dados Scopus, Scifinder, Medline, Web of Science e nas bibliotecas eletrônicas Sciencedirect e Scielo, no período compreendido entre 2013 e 2019. Como resultados, foram selecionados um total de 18 artigos, após aplicação dos critérios de inclusão e exclusão. Dos estudos encontrados, os que foram identificados em maior quantidade estão relacionados a atividade antimicrobiana. Bromelia pinguin, Bromelia laciniosa, Neoglaziovia variegata, Encholirium spectabile, entre outras espécies, apresentaram atividade microbicida frente a bactérias Gram-positivas e Gram-negativas, entre elas Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa e Escherichia coli. Alguns estudos também demonstraram a atividade anti-inflamatória de Bromelia balansae, antiulcerogênica de Ananas ananassoides e antirreumática e antioxidante de Encholirium spectabile. Além das propriedades mencionadas, foi identificado o potencial fotoprotetor, antinociceptivo, antiofídico, anticoagulante entre outras, e foi estabelecida a relação entre estas atividades e a presença de metabólitos secundários. A partir das buscas na literatura foi possível encontrar numerosas pesquisas que respaldam a utilização e o estudo contínuo de plantas como método alternativo às práticas medicinais atuais. Com este estudo, deseja-se contribuir para a ampliação do conhecimento a respeito do potencial biológico da família Bromeliaceae.
    Keywords bromeliaceae ; atividade antimicrobiana ; encholirium spectabile ; plantas medicinais ; ; Special aspects of education ; LC8-6691 ; Science (General) ; Q1-390 ; Social Sciences ; H
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Murine Experimental Root Canal Infection: Cytokine Expression in Response to F. nucleatum and E. faecalis.

    Reis, Andressa Lamari / Oliveira, Ricardo Reis / Tavares, Warley Luciano Fonseca / Saldanha, Thamyris Duque Silva / Farias, Luiz de Macêdo / Vieira, Leda Quércia / Ribeiro, Antônio Paulino

    Brazilian dental journal

    2016  Volume 27, Issue 5, Page(s) 578–583

    Abstract: ... association of Fusobacterium nucleatum (ATCC 10953) and Enterococcus faecalis (ATCC 19433). F. nucleatum and E ... real-time PCR. The Kruskal-Wallis test was used for statistical analysis. F. nucleatum mono-infection ... mechanism via IL-10 and TGF-b. Bi-association (F. nucleatum and E. faecalis) stimulated high expression ...

    Abstract The aim of this study was to evaluate the gene expression of proinflammatory (RANKL, TNF-a and IFN-g) and regulatory (TGF-b and IL-10) cytokines as reaction to experimental infection by mono or bi-association of Fusobacterium nucleatum (ATCC 10953) and Enterococcus faecalis (ATCC 19433). F. nucleatum and E. faecalis, either in mono- or bi-association were inoculated into the root canal system (RCS) of Balb/c mice. Animals were sacrificed at 10 and 20 days after infection and periapical tissues surrounding the root were collected. The mRNA expression of the cytokines RANKL, TNF-a, IFN- g, TGF-b and IL-10 was assessed using real-time PCR. The Kruskal-Wallis test was used for statistical analysis. F. nucleatum mono-infection induced high expression of RANKL and TNF-a, while its modulation was due to IL-10. High expression of IFN-g at day 20 was up-regulated by E. faecalis and RANKL; TNF-a was up-regulated by an independent mechanism via IL-10 and TGF-b. Bi-association (F. nucleatum and E. faecalis) stimulated high expression of RANKL, TNF-a and IFN-g, which seemed to be modulated by TGF-b 20 days later. The gene expression of proinflammatory cytokines was more prominent in the earlier periods of the experimental periapical infection, which concomitantly decreased in the later period. This expression may be regulated by IL-10 and TGF-b in an infection-specific condition.
    MeSH term(s) Animals ; Cytokines/metabolism ; Dental Pulp Cavity/metabolism ; Dental Pulp Cavity/pathology ; Enterobacter/metabolism ; Fusobacterium nucleatum/metabolism ; Mice ; Mice, Inbred BALB C
    Chemical Substances Cytokines
    Language English
    Publishing date 2016-09
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 1089484-6
    ISSN 1806-4760 ; 0103-6440
    ISSN (online) 1806-4760
    ISSN 0103-6440
    DOI 10.1590/0103-6440201600926
    Database MEDical Literature Analysis and Retrieval System OnLINE

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