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Article: YIN Kejing

Tong, Yue / DU, Xu / Yin, Kejing

Zhongguo zhen jiu = Chinese acupuncture & moxibustion

2024 Volume 44, Issue 2, Page(s) 185–187

Abstract: The paper introduces ... ...

Title translation	殷克敬傍刺天池穴治疗乳腺增生症临床经验.
Abstract	The paper introduces professor
MeSH term(s)	Percutaneous Collagen Induction ; Medicine, Chinese Traditional ; Meridians ; Acupuncture Therapy
Language	Chinese
Publishing date	2024-02-12
Publishing country	China
Document type	Journal Article
ISSN	0255-2930
ISSN	0255-2930
DOI	10.13703/j.0255-2930.20230504-0003
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Article: Dan-Shen-Yin Granules Prevent Hypoxia-Induced Pulmonary Hypertension

Wang, Ran-Ran / Yuan, Tian-Yi / Chen, Di / Chen, Yu-Cai / Sun, Shu-Chan / Wang, Shou-Bao / Kong, Ling-Lei / Fang, Lian-Hua / Du, Guan-Hua

Frontiers in pharmacology

2022 Volume 13, Page(s) 844400

Abstract: ... of their multiple components. The present study aims to evaluate the effects of Dan-Shen-Yin (DSY) granules ...

Abstract	Traditional Chinese medicine (TCM) plays an important role in the treatment of complex diseases, especially cardiovascular diseases. However, it is hard to identify their modes of action on account of their multiple components. The present study aims to evaluate the effects of Dan-Shen-Yin (DSY) granules on hypoxia-induced pulmonary hypertension (HPH), and then to decipher the molecular mechanisms of DSY. Systematic pharmacology was employed to identify the targets of DSY on HPH. Furthermore, core genes were identified by constructing a protein-protein interaction (PPI) network and analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes (KEGG) analysis. Related genes and pathways were verified using a hypoxia-induced mouse model and hypoxia-treated pulmonary artery cells. Based on network pharmacology, 147 potential targets of DSY on HPH were found, constructing a PPI network, and 13 hub genes were predicted. The results showed that the effect of DSY may be closely associated with AKT serine/threonine kinase 1 (AKT1), signal transducer and activator of transcription 3 (STAT3), and HIF-1 signaling pathways, as well as biological processes such as cell proliferation. Consistent with network pharmacology analysis, experiments
Language	English
Publishing date	2022-03-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.844400
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Article ; Online: Molecular mechanism of Gan-song Yin inhibiting the proliferation of renal tubular epithelial cells by regulating miR-21-5p in adipocyte exosomes.

Xu, Duojie / Yuan, Ling / Che, Mengying / Lu, Doudou / Liu, Wenjing / Meng, Fandi / Yang, Yating / Du, Yuhua / Hou, Shaozhang / Nan, Yi

Journal of ethnopharmacology

2023 Volume 321, Page(s) 117530

Abstract: Ethnopharmacological relevance: Gan-song Yin is derived from the classic ancient prescription ...

Abstract	Ethnopharmacological relevance: Gan-song Yin is derived from the classic ancient prescription " Gan-song pill " for the treatment of wasting-thirst in Ningxia combined with the characteristic "fragrant medicine". It is clinically used for the treatment of early renal fibrosis caused by diabetic nephropathy. Previous studies have shown that it has a good effect and great potential in the prevention and treatment of diabetic nephropathy, but its mechanism research is still limited. Aim of the study: To investigate the mechanism of GSY to improve DN by interfering with miR-21-5p and glycolipid metabolism in adipocyte exosomes using 3T3-L1 and TCMK-1 co-culture system. Materials and methods: The co-culture system of 3T3-L3 and TCMK-1 was established, the IR model was established, and the stability, lipid drop change, glucose consumption, triglyceride content, cell viability, cell cycle and apoptosis level, protein content and mRNA expression of the IR model were detected. Results: GSY inhibited 3T3-L1 activity, increased glucose consumption and decreased TG content. Decreased TCMK-1 cell viability, inhibited apoptosis, cell cycle arrest occurred in G0/G1 phase and S phase. Adipocyte IR model and co-culture system were stable within 48 h. After GSY intervention, lipid droplet decomposition and glucose consumption increased. The TG content of adipocytes increased, while the TG content of co-culture system decreased. GSY can regulate the expression of TGF-β1/SMAD signaling pathway protein in IR state. After GSY intervention, the expression of miR-21-5p was increased in 3T3-L1 and Exo cells, and decreased in TCMK-1 cells. Conclusions: GSY can regulate TGF-β1/SMAD signaling pathway through the secretion of miR-21-5p from adipocytes, protect IR TCMK-1, regulate the protein and mRNA expression levels of PPARγ, GLUT4, FABP4, and improve glucose and lipid metabolism.
MeSH term(s)	Humans ; Transforming Growth Factor beta1/metabolism ; Exosomes/metabolism ; Diabetic Nephropathies/metabolism ; Adipocytes ; Cell Proliferation ; Epithelial Cells/metabolism ; Glucose/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; RNA, Messenger/metabolism
Chemical Substances	Transforming Growth Factor beta1 ; Glucose (IY9XDZ35W2) ; MicroRNAs ; RNA, Messenger ; MIRN21 microRNA, human
Language	English
Publishing date	2023-12-01
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117530
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Zs.A 1494: Show issues

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Article ; Online: Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats.

Fu, San / Song, Xianrui / Tang, Xiaoyan / Qian, Xiuhui / Du, Zesen / Hu, Yingying / Xu, Xianghong / Zhang, Mian

Journal of ethnopharmacology

2022 Volume 306, Page(s) 116050

Abstract: ... Yangyinyiqi (YY) and Lishijianpi (LS) groups. A rat model of Yin-deficiency pulmonary fibrosis induced ... of lung tissue and the changes of biomarkers associated with fibrosis, Yin-deficiency and water-fluid ... The average CC on Yin-deficiency indexes was 0.25, and YY group had the highest RPV (0.96) in improving ...

Abstract	Ethnopharmacological relevance: Baibutang (BBT) is an ancient prescription for the treatment of pulmonary fibrosis. Previous experiments have shown that BBT had a good therapeutic effect on pulmonary fibrosis. However, there had been no study on the synergy between drugs composed of BBT. Due to the interaction between the constituent drugs, exploring their synergy profile is of great significance for explaining the essence of BBT's efficacy in improving pulmonary fibrosis. Aim of the study: Based on the pharmacodynamic value, this study aimed to explore a method for the evaluation of the synergy profile between constituent drugs in traditional Chinese medicine (TCM) compounds. Materials and methods: Nine herbs of BBT were divided into Zhikeqingre (ZK), Yangyinyiqi (YY) and Lishijianpi (LS) groups. A rat model of Yin-deficiency pulmonary fibrosis induced by thyroxine-bleomycin was used to evaluate the effects of BBT and the three groups. The pathological changes of lung tissue and the changes of biomarkers associated with fibrosis, Yin-deficiency and water-fluid metabolism were detected. After standardization of pharmacodynamics value (PV), the compatibility coefficient (CC) of the three groups, the relative PV (RPV) and contribution value (CV) of each group on every index were calculated. Results: The average CC on fibrosis indexes was 0.44, indicating that 44% of the efficacy of BBT came from the synergistic effect of the three groups. ZK group had the highest RPV (0.80) in improving fibrosis indexes such as histopathological changes, α-SMA, collagen-I and renin-angiotensin system. The average CC on Yin-deficiency indexes was 0.25, and YY group had the highest RPV (0.96) in improving deficiency indexes such as body temperature, cAMP/cGMP ratio, and PDEs, PGE2 and COX-2 levels. The average CC on water-fluid metabolism indexes was 0.15, and LS group had the highest RPV (1.52) in improving water-fluid metabolism indexes such as aquaporins, mucins, and surfactant proteins. The results also showed that 29% of the improvement effect of BBT on all indexes came from the synergistic effect of the three groups, and the contribution of ZK, YY and LS groups to the efficacy of BBT were 25%, 25% and 21%, respectively. Conclusion: The established semiquantitative method can clearly and simply evaluate the synergy of the three groups in BBT, which will help to promote the research on the synergy of TCM compounds and other multiple-components combinations.
MeSH term(s)	Rats ; Animals ; Pulmonary Fibrosis/drug therapy ; Drugs, Chinese Herbal/therapeutic use ; Yin Deficiency/drug therapy ; Medicine, Chinese Traditional ; Lung
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2022-12-17
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.116050
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Article: [Effect of Dendrobium officinale superfine powder on stomach Yin deficiency model mice induced by "spicy overeating"].

Chen, Qing-Qing / Su, Jie / DU, Yu-Zhong / Sun, Chen-Chen / Chen, Su-Hong / Lyu, Gui-Yuan

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2021 Volume 46, Issue 7, Page(s) 1651–1657

Abstract: Dendrobium officinale is a traditional Chinese medicine for nourishing Yin and benefiting stomach ... Yin deficiency model are still not sufficient. In this experiment, we explored the effect of D. officinale ... superfine powder in mice model with stomach Yin deficiency caused by "spicy overeating", and provided ...

Abstract	Dendrobium officinale is a traditional Chinese medicine for nourishing Yin and benefiting stomach. Its superfine powder has many advantages, such as good dissolution, high utilization rate, strong integrity and easy to use. However, the researches on effect of D. officinale superfine powder on stomach Yin deficiency model are still not sufficient. In this experiment, we explored the effect of D. officinale superfine powder in mice model with stomach Yin deficiency caused by "spicy overeating", and provided certain reference value for its application in gastrointestinal diseases. Male ICR mice were randomly divided into normal group, model group, Yiweitang group, omeprazole group, and D. officinale superfine powder high, medium and low dose groups. The mixture of wine and pepper liquid was given by gavage administration for 30 d, and the corresponding drug was given for 60 d while the model was conti-nued. The body weight, food intake, water intake, fecal moisture content and particle number, foot temperature of mice were measured. The levels of serum gastrin(Gas), motilin(MTL) and somatostatin(SS) were measured by ELISA. Gastric histomorpho-logy was observed by HE staining. The expression levels of nuclear factor kappa B(NF-κB) and cyclooxygenase-2(COX-2) were determined by immunohistochemistry. The expression levels of B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax) in gastric tissues were detected by Western blot. The results showed that D. officinale superfine powder could increase the food intake, water intake, fecal moisture content and particle number, reduce the foot temperature, improve the pathological changes of gastric mucosa, reduce the expression of NF-κB, COX-2 protein in gastric tissues, and increase the ratio of Bax/Bcl-2. D. officinale superfine powder can "nourish Yin and benefit the stomach", improve the syndrome of stomach Yin deficiency, such as "hunger but not want to eat, dry mouth but not want to drink, hand and feet hot, constipation", and reduce the damage of gastric mucosa. The mechanism may be related to regulating the secretion of gastrointestinal hormones, inhibiting the inflammation of gastric tissues and promoting the apoptosis of abnormal cells in gastric tissues.
MeSH term(s)	Animals ; Dendrobium ; Hyperphagia ; Male ; Mice ; Mice, Inbred ICR ; Powders ; Stomach ; Yin Deficiency
Chemical Substances	Powders
Language	Chinese
Publishing date	2021-05-12
Publishing country	China
Document type	Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20200810.403
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Zs.A 3056: Show issues

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Article: Reduced MLH3 Expression in the Syndrome of Gan-Shen Yin Deficiency in Patients with Different Diseases.

Du, Juan / Zhong, Maofeng / Liu, Dong / Liang, Shufang / Liu, Xiaolin / Cheng, Binbin / Zhang, Yani / Yin, Zifei / Wang, Yuan / Ling, Changquan

Evidence-based complementary and alternative medicine : eCAM

2017 Volume 2017, Page(s) 4109828

Abstract: ... to certain diseases. This study aimed to assess the characteristic genes of gan-shen Yin ... and 100 diabetes mellitus (DM) patients were enrolled and classified into gan-shen Yin ... deficiency group and non-gan-shen Yin deficiency group according to the body constitution questionnaire to assess ...

Abstract	Traditional Chinese medicine formulates treatment according to body constitution (BC) differentiation. Different constitutions have specific metabolic characteristics and different susceptibility to certain diseases. This study aimed to assess the characteristic genes of gan-shen Yin deficiency constitution in different diseases. Fifty primary liver cancer (PLC) patients, 94 hypertension (HBP) patients, and 100 diabetes mellitus (DM) patients were enrolled and classified into gan-shen Yin deficiency group and non-gan-shen Yin deficiency group according to the body constitution questionnaire to assess the clinical manifestation of patients. The mRNA expressions of 17 genes in PLC patients with gan-shen Yin deficiency were different from those without gan-shen Yin deficiency. However, considering all patients with PLC, HBP, and DM, only MLH3 was significantly lower in gan-shen Yin deficiency group than that in non-gen-shen Yin deficiency. By ROC analysis, the relationship between MLH3 and gan-shen Yin deficiency constitution was confirmed. Treatment of MLH3 (-/- and -/+) mice with Liuweidihuang wan, classical prescriptions for Yin deficiency, partly ameliorates the body constitution of Yin deficiency in MLH3 (-/+) mice, but not in MLH3 (-/-) mice. MLH3 might be one of material bases of gan-shen Yin deficiency constitution.
Language	English
Publishing date	2017-09-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2017/4109828
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Zs.A 5995: Show issues

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Article ; Online: Comprehensive chemical profiling of Jia-Wei-Qi-Fu-Yin and its network pharmacology-based analysis on Alzheimer's disease.

An, Hai-Ming / Huang, Da-Rong / Yang, Hua / Liu, Xin-Guang / Du, Jing / Li, Yi / Li, Chao-Ran / Pang, Han-Qing / Liu, Run-Zhou / Peng, Chao / Li, Ping / Gao, Wen

Journal of pharmaceutical and biomedical analysis

2020 Volume 189, Page(s) 113467

Abstract: Jia-Wei-Qi-Fu-Yin (JWQFY) is a newly developed anti-Alzheimer's disease (AD) prescription modified ... from a classical traditional Chinese medicine formula, Qi-Fu-Yin (QFY). However, a systematic understanding ...

Abstract	Jia-Wei-Qi-Fu-Yin (JWQFY) is a newly developed anti-Alzheimer's disease (AD) prescription modified from a classical traditional Chinese medicine formula, Qi-Fu-Yin (QFY). However, a systematic understanding of its chemical constituents and molecular mechanisms is still elusive. To address this problem, comprehensive chemical profiling followed by network pharmacology-based analysis of JWQFY was performed. Firstly, a total of 136 compounds were characterized by high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF MS), 17 of them were specifically identified in JWQFY comparing with QFY. Seventy compounds were further quantified via a validated HPLC coupled with triple quadrupole tandem mass spectrometry (QQQ MS) method. Then the protein targets of the seventy compounds were gathered from public databases for network construction. As a result, fifty-seven compounds were filtered, which interacted with 655 targets. Thirty-four of them were mapped into the KEGG pathway of AD, indicating JWQFY might exert anti-AD effects by anti-inflammation, neuronal apoptosis intervening, Aβ production inhibition and phosphorylating tau protein moderating. Furthermore, in the compound-target-AD network, a list of hub compounds and hub targets was identified based on their topological features, including the degree, node betweenness and closeness. Four of the hub compounds were specifically originated from JWQFY, supporting the modification rationality of this formula. This study provided a scientific basis for understanding the bioactive compounds and the multi-target mechanism of JWQFY.
MeSH term(s)	Alzheimer Disease/drug therapy ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; Humans ; Tandem Mass Spectrometry
Chemical Substances	Drugs, Chinese Herbal ; qi-fu-yin
Language	English
Publishing date	2020-07-08
Publishing country	England
Document type	Journal Article
ZDB-ID	604917-5
ISSN	1873-264X ; 0731-7085
ISSN (online)	1873-264X
ISSN	0731-7085
DOI	10.1016/j.jpba.2020.113467
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Article ; Online: Effect of You-Gui Yin on glucocorticoid-induced apoptosis of bone marrow mesenchymal stem cells

Hao Chen / Bin Du

Journal of Hainan Medical University, Vol 27, Iss 24, Pp 11-

2021 Volume 16

Abstract: Objective: To investigate the effect of You-Gui Yin on glucocorticoid-induced apoptosis of rat ... saline group and You-Gui Yin group. Ten rats in each group were given normal saline and You-Gui Yin ...

Abstract	Objective: To investigate the effect of You-Gui Yin on glucocorticoid-induced apoptosis of rat bone marrow mesenchymal stem cells and its possible mechanism. Methods: 20 SD rats were divided into normal saline group and You-Gui Yin group. Ten rats in each group were given normal saline and You-Gui Yin by gavage for 2 weeks, once daily. After the gavage, the rats were sacrificed by spinal removal, blood was taken from the abdominal aorta and centrifuged to obtain blank serum and medicated serum. The rat bone marrow mesenchymal stem cells were extracted and cultured and administered in groups: blank group (10% blank serum) , Model group (10% blank serum + dexamethasone 5×10-5mol/ml), traditional Chinese medicine group (10% medicated serum + dexamethasone 5×10-5mol/ml), control group (10% medicated serum), CCK-8 method was used to detect cell proliferation changes in different groups, ELISA method was used to detect bone alkaline phosphatase levels in each group, Alizarin red staining was used to compare red-stained calcium mineralized nodules in each group, Western Blot Detect the expression of apoptosis-related proteins in BMSCs, and perform molecular docking of the quantitative components to evaluate the binding strength and activity of the target and the active compound. Results: The ratio of absorbance (A450) in each time period of the model group was significantly reduced (P<0.05), and the ratio of absorbance (A450) between the traditional Chinese medicine group and the control group was significantly higher than that of the model group (P<0.05). There was no difference between the control group and the blank group (P>0.05); ELISA method showed that the ALP level of the model group was significantly lower than that of the blank group and the control group (P<0.05), the traditional Chinese medicine group significantly increased the ALP level (P<0.05), there was no significant difference between the control group and the blank group; Observation of the number of calcium mineralized nodules under ...
Keywords	you-gui yin ; bone marrow mesenchymal stem cells ; apoptosis ; target protein ; molecular docking ; Medicine ; R
Subject code	630
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Editorial Board of Journal of Hainan Medical University
Document type	Article ; Online
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Article ; Online: UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine.

Wang, Xin / Du, Yue / Wu, Cuiting / Xu, Ming / Liu, Youping / Di, Xin

Journal of pharmaceutical analysis

2020 Volume 11, Issue 4, Page(s) 458–464

Abstract: ... considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine ... in normal and Yin deficiency diabetic rats treated with or without ...

Abstract	Cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) are considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine. However, phosphodiesterase-mediated ex vivo degradation of these molecules in biological samples may result in their underestimation. In the present study, a ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for determination of cAMP and cGMP in rat plasma, with special consideration of their stability ex vivo. Following precipitation of proteins from plasma samples with 0.4 M perchloric acid, the analytes were chromatographed on a Shimadzu Shim-pack-XR-ODS II column with 2.5 mM ammonium acetate and methanol in gradient mode. The MS/MS detection was performed using multiple reaction monitoring in the positive electrospray ionization mode. The lower limit of quantification was 0.27 ng/mL for cAMP and 0.37 ng/mL for cGMP. The method was used to determine the plasma cAMP and cGMP levels in normal and Yin deficiency diabetic rats treated with or without
Language	English
Publishing date	2020-09-05
Publishing country	China
Document type	Journal Article
ZDB-ID	2630174-X
ISSN	2214-0883 ; 2095-1779
ISSN (online)	2214-0883
ISSN	2095-1779
DOI	10.1016/j.jpha.2020.09.001
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Article ; Online: Dan-Shen-Yin Granules Prevent Hypoxia-Induced Pulmonary Hypertension via STAT3/HIF-1α/VEGF and FAK/AKT Signaling Pathways

Ran-Ran Wang / Tian-Yi Yuan / Di Chen / Yu-Cai Chen / Shu-Chan Sun / Shou-Bao Wang / Ling-Lei Kong / Lian-Hua Fang / Guan-Hua Du

Frontiers in Pharmacology, Vol

2022 Volume 13

Abstract: ... of their multiple components. The present study aims to evaluate the effects of Dan-Shen-Yin (DSY) granules ...

Abstract	Traditional Chinese medicine (TCM) plays an important role in the treatment of complex diseases, especially cardiovascular diseases. However, it is hard to identify their modes of action on account of their multiple components. The present study aims to evaluate the effects of Dan-Shen-Yin (DSY) granules on hypoxia-induced pulmonary hypertension (HPH), and then to decipher the molecular mechanisms of DSY. Systematic pharmacology was employed to identify the targets of DSY on HPH. Furthermore, core genes were identified by constructing a protein-protein interaction (PPI) network and analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes (KEGG) analysis. Related genes and pathways were verified using a hypoxia-induced mouse model and hypoxia-treated pulmonary artery cells. Based on network pharmacology, 147 potential targets of DSY on HPH were found, constructing a PPI network, and 13 hub genes were predicted. The results showed that the effect of DSY may be closely associated with AKT serine/threonine kinase 1 (AKT1), signal transducer and activator of transcription 3 (STAT3), and HIF-1 signaling pathways, as well as biological processes such as cell proliferation. Consistent with network pharmacology analysis, experiments in vivo demonstrated that DSY could prevent the development of HPH in a hypoxia-induced mouse model and alleviate pulmonary vascular remodeling. In addition, inhibition of STAT3/HIF-1α/VEGF and FAK/AKT signaling pathways might serve as mechanisms. Taken together, the network pharmacology analysis suggested that DSY exhibited therapeutic effects through multiple targets in the treatment of HPH. The inferences were initially confirmed by subsequent in vivo and in vitro studies. This study provides a novel perspective for studying the relevance of TCM and disease processes and illustrates the advantage of this approach and the multitargeted anti-HPH effect of DSY.
Keywords	traditional chinese medicine ; Dan-Shen-Yin ; hypoxia-induced pulmonary hypertension ; network pharmacology ; mechanism ; Therapeutics. Pharmacology ; RM1-950
Subject code	610
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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