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Article ; Online: African swine fever continues to be a problem in Romania and Timiș county

Daniela Marina Mot / Emil Tîrziu

Scientific Papers Animal Science and Biotechnologies, Vol 55, Iss 1, Pp 261-

2022 Volume 269

Abstract: African swine fever continues to evolve in Europe and in our country, even in the last two years of the pandemic, and is currently the main threat to the global swine industry. It affects more than 50 countries on 5 continents, with several ... ...

Abstract	African swine fever continues to evolve in Europe and in our country, even in the last two years of the pandemic, and is currently the main threat to the global swine industry. It affects more than 50 countries on 5 continents, with several epidemiological scenarios. Even this year, Romania was not bypassed by this serious disease, which causes many economic damages: in the case of domestic pigs, in the EU Member States were registered, between 01.01-16.01.2022, 24 outbreaks, of which 22 in Romania. In the same period, 565 outbreaks of PPA were reported in wild boars. Most evolve in Poland (177), Bulgaria (120), Germany (78) and Romania (59). Europe is waiting for an effective vaccine against African swine fever (PPA) by 2024, the main risk to pig health worldwide, being the only way to eradicate the disease. Veterinary services continue their efforts to prevent the spread of the African swine fever virus (PPA), those for the management of disease outbreaks, with the aim of reducing outbreaks and spreading this disease, which already has a history in our country, evolving since 2017 almost constantly.
Keywords	african swine fever ; epidemiological ; outbreaks ; boars ; Agriculture ; S ; Technology ; T ; Science ; Q
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Agroprint Timisoara
Document type	Article ; Online
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Article ; Online: Supervised Exercise Therapy Versus Percutaneous Transluminal Angioplasty for Claudication by Level of Disease: A Systematic Review and Meta-Analysis.

Kapadia, Sohum / Huang, Haocheng / Parise, Helen / Ismayl, Mahmoud / Ahmed, Zain / Tirziu, Daniela / Altin, S Elissa

The American journal of cardiology

2023 Volume 205, Page(s) 403–405

MeSH term(s)	Humans ; Angioplasty ; Exercise Therapy
Language	English
Publishing date	2023-08-31
Publishing country	United States
Document type	Meta-Analysis ; Systematic Review ; Journal Article
ZDB-ID	80014-4
ISSN	1879-1913 ; 0002-9149
ISSN (online)	1879-1913
ISSN	0002-9149
DOI	10.1016/j.amjcard.2023.08.044
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Article ; Online: Bird flu

Daniela Marina Mot / Ileana Nichita / Emil Tîrziu / Teodor Moț

Scientific Papers Animal Science and Biotechnologies, Vol 53, Iss 2, Pp 289-

new outbreaks but an old problem for intensive European poultry

2020 Volume 301

Abstract: Bird flu or avian influenza continues to cause problems at European level, with new outbreaks of disease being reported, including those identified in January 2020 in Romania. It was first identified in the 1900s in Italy, but was later found worldwide. ... ...

Abstract	Bird flu or avian influenza continues to cause problems at European level, with new outbreaks of disease being reported, including those identified in January 2020 in Romania. It was first identified in the 1900s in Italy, but was later found worldwide. An avian influenza virus, H5N1, was identified in 1997 in Asia and created worldwide panic, talking about the imminence of a major epidemic. In October 2005, the H5N1 virus also arrived in Romania, being identified in birds from Ceamurlia de Jos, Tulcea County. Romania is the first European country where the presence of this virus has been officially confirmed. Avian influenza has attracted the attention of the international community over the years, with outbreaks in the poultry industry or livelihoods having serious consequences on both livelihoods and international trade in many countries. Apart from Romania, avian influenza is still evolving in Poland, Slovakia, Hungary, Germany, Ukraine and Northern Ireland. Preventing the occurrence and combating avian influenza can only be achieved through international cooperation and collaboration, in order to know at any time the epidemiological situation and the pathogenic capacity of the etiological agent. Continuous surveillance of avian influenza virus in wild birds is very important, as it provides us with an "early warning" about the introduction of the virus into new regions, and can conduct appropriate risk assessment studies. It is also important to understand the behavior of wild birds, possibly the transmission of the virus between wild and domestic birds, the age categories of the frequently affected wild birds, the migration routes. At the same time, it is very difficult to prevent the occurrence of avian influenza in domestic birds due to the fact that a strategy for controlling wild birds cannot be established, the only solution to prevent the occurrence of the disease being the maximum limitation of contact between domestic and wild birds.
Keywords	bird flu ; avian influenza ; strategy for controlling ; disease ; outbreaks ; Agriculture ; S ; Technology ; T ; Science ; Q
Subject code	320
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	Agroprint Timisoara
Document type	Article ; Online
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Article ; Online: The evolution of African swine fever (ASF) in Europe and our country

Daniela Marina Mot / Ileana Nichita / Emil Tîrziu / Teodor Moț

Scientific Papers Animal Science and Biotechnologies, Vol 52, Iss 1, Pp 146-

2019 Volume 158

Abstract: African swine fever (ASF) is a viral infectious disease specific to domestic and wild swine of all ages, highly contagious and very serious, probably the most dangerous and damaging disease of pigs, able to produce in new outbreaks of domestic swine ... ...

Abstract	African swine fever (ASF) is a viral infectious disease specific to domestic and wild swine of all ages, highly contagious and very serious, probably the most dangerous and damaging disease of pigs, able to produce in new outbreaks of domestic swine fever a mortality of up to 100%. This disease is produced by a deoxyribovirus of the genus Asfavirus, the family of Asfaviridae with at least 22 different genomic types. In Europe, the disease was first reported in 1957 in Portugal, around Lisbon. Although the first outbreaks were declared eradicated, in 1960 others appeared when the disease spread to Spain and after that it could not be eradicated until 1990, after great efforts and huge losses. ASF was also reported in other European countries as: France (1964), Italy (1967), Malta (1978), Belgium (1985), The Netherlands (1986). Of all this, ASF could be eradicated by extremely drastic and costly measures, except in Sardinia, where it would seem to persist in today's endemic form. In the middle of 2007 this disease appeared in countries of the former Soviet Union and in the Czech Republic, then in 2016 in Poland, in 2017 in Romania, Hungary and Belgium. In 2018 ASF spread in Bulgaria and Italy. The updated map of European Union outbreaks of swine fever in the European Union shows that Romania is the country most affected by the virus, with almost half of the counties in the red zone - officially discovered pests.
Keywords	african swine fever ; deoxyribovirus ; disease ; outbreaks ; Agriculture ; S ; Technology ; T ; Science ; Q
Subject code	940
Language	English
Publishing date	2019-05-01T00:00:00Z
Publisher	Agroprint Timisoara
Document type	Article ; Online
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Article ; Online: Long term outcomes of ultrathin versus standard thickness second-generation drug eluting stents: Meta-analysis of randomized trials.

Hussain, Yasin / Gaston, Samantha / Kluger, Johnathan / Shah, Tayyab / Yang, Yiping / Tirziu, Daniela / Lansky, Alexandra

Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions

2021 Volume 99, Issue 3, Page(s) 563–574

Abstract: Objective: Identify the effect of ultrathin drug eluting stents on long term outcomes in coronary artery disease.: Background: Although second-generation drug eluting stents (DES) are superior to first-generation DES, persistence of adverse outcomes ... ...

Abstract	Objective: Identify the effect of ultrathin drug eluting stents on long term outcomes in coronary artery disease. Background: Although second-generation drug eluting stents (DES) are superior to first-generation DES, persistence of adverse outcomes has led to continued refinement in design. Ultrathin second-generation DES have been shown to improve outcomes at 1-year follow-up. Beyond 1-year their effect remains unknown. Methods: PubMed, Embase and Cochrane Database were searched for randomized controlled trials that compared ultrathin (defined as <70 um) to standard thickness second-generation DES. Studies were chosen according to the PROSPERO protocol (CRD42020185374). Data from randomized controlled trials were pooled using random-effects model (Mantel-Haenszel). The primary outcome was target lesion failure (TLF) at 2 years, a composite of cardiac death, target vessel myocardial infarction, and ischemia-driven target vessel revascularization. Secondary outcomes included TLF at 3 and 5 years, the components of TLF and definite or probable stent thrombosis. Differences in outcomes between groups were presented in Forest plots as risk ratios (RR) with corresponding 95% confidence intervals (CIs) for each trial. Results: We identified 18 publications from 10 trials with14,649 patients. At 2-years there was a significant 12% reduction in TLF (RR, 0.88; 95% CI 0.78-0.99; p < 0.05) associated with the use of ultrathin DES. At 3-years, there was a significant 19% reduction in TLF with ultrathin DES (RR, 0.79; 95% CI 0.64-0.98; p < 0.05). Conclusion: In patients undergoing percutaneous coronary intervention, ultrathin DES improve long term clinical outcomes.
MeSH term(s)	Coronary Artery Disease/diagnostic imaging ; Coronary Artery Disease/etiology ; Coronary Artery Disease/therapy ; Drug-Eluting Stents/adverse effects ; Humans ; Percutaneous Coronary Intervention/adverse effects ; Prosthesis Design ; Randomized Controlled Trials as Topic ; Risk Factors ; Treatment Outcome
Language	English
Publishing date	2021-07-08
Publishing country	United States
Document type	Journal Article ; Meta-Analysis
ZDB-ID	1459995-8
ISSN	1522-726X ; 1522-1946
ISSN (online)	1522-726X
ISSN	1522-1946
DOI	10.1002/ccd.29866
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Zs.A 1226: Show issues

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Article ; Online: Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization.

Memi, Fani / Tirziu, Daniela / Papangeli, Irinna

Journal of visualized experiments : JoVE

2018 , Issue 139

Abstract: micro-RNAs (miRNAs) are single-stranded RNA transcripts that bind to messenger RNAs (mRNAs) and inhibit their translation or promote their degradation. To date, miRNAs have been implicated in a large number of biological and disease processes, which has ... ...

Abstract	micro-RNAs (miRNAs) are single-stranded RNA transcripts that bind to messenger RNAs (mRNAs) and inhibit their translation or promote their degradation. To date, miRNAs have been implicated in a large number of biological and disease processes, which has signified the need for the reliable detection methods of miRNA transcripts. Here, we describe a detailed protocol for digoxigenin-labeled (DIG) Locked Nucleic Acid (LNA) probe-based miRNA detection, combined with protein immunostaining on mouse heart sections. First, we performed an in situ hybridization technique using the probe to identify miRNA-182 expression in heart sections from control and cardiac hypertrophy mice. Next, we performed immunostaining for cardiac Troponin T (cTnT) protein, on the same sections, to co-localize miRNA-182 with the cardiomyocyte cells. Using this protocol, we were able to detect miRNA-182 through an alkaline phosphatase based colorimetric assay, and cTnT through fluorescent staining. This protocol can be used to detect the expression of any miRNA of interest through DIG-labeled LNA probes, and relevant protein expression on mouse heart tissue sections.
MeSH term(s)	Animals ; In Situ Hybridization/methods ; Mice ; MicroRNAs/metabolism
Chemical Substances	MicroRNAs
Language	English
Publishing date	2018-09-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/57920
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Article ; Online: Bluetongue in Europe and Romania in the last years

Daniela Marina Mot / Ileana Nichita / Emil Tîrziu / Teodor Moț

Scientific Papers Animal Science and Biotechnologies, Vol 51, Iss 1, Pp 203-

2018 Volume 213

Abstract: Beginning with 2006, in Europe had been a lot of Bluetongue (BT) outbreaks evolving like hemorrhagic disease in ruminants, caused by bluetongue virus (BTV). This paper reviews an epidemiological situation of this disease in Europe and in Romania in the ... ...

Abstract	Beginning with 2006, in Europe had been a lot of Bluetongue (BT) outbreaks evolving like hemorrhagic disease in ruminants, caused by bluetongue virus (BTV). This paper reviews an epidemiological situation of this disease in Europe and in Romania in the last years with all health and economic problems that followed the outbreaks. For the first time described in 1905 in Merino sheep from South Africa, BTV is an Arbovirus isolated from wild and domestic ruminants, with sublinical to fatal symptoms of disease. The name Bluetongue derived from necrosis, cell injuries with vascular thrombosis, oedema, haemorrhages that make the tongue cyanotic, with a blue colour. By historically point of view BTV is known to be prevalent in tropical and subtropical areas between 35̊ S and 45̊ N, but many outbreaks were than reported in further northwards, an emergence may be caused by climate changes, when a high temperature contributes to both distribution and competence of certain species of Culicoides midges, known to be the insect vector of BT disease. With the exception of Antarctica BTV was been isolated on every continent. From 1,400 species of Culicoides throught the world, only about 30 species have been discovered to be competent biological vectors. There are more regions in Europe believed previously to be not a risk for bluetongue evolution but the situation since 2006 demonstrates the contrary.
Keywords	bluetongue ; epidemiological situation ; outbreaks ; biological vector ; Agriculture ; S ; Technology ; T ; Science ; Q
Language	English
Publishing date	2018-05-01T00:00:00Z
Publisher	Agroprint Timisoara
Document type	Article ; Online
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Article ; Online: Impact of sex on Transcatheter aortic valve replacement outcomes: Results of a single-center study.

See, Claudia / Wang, Yanting / Yang, Yiping / Tirziu, Daniela / Papoutsidakis, Nikolaos / Francese, Dominic P / Kaple, Ryan K / Cleman, Michael / Lansky, Alexandra J / Forrest, John K

International journal of cardiology

2023 Volume 398, Page(s) 131643

Abstract: Background: Early studies on transcatheter aortic valve replacement (TAVR) outcomes showed that female sex was associated with better survival. With increased use of new-generation valves, the impact of sex on contemporary TAVR outcomes is less well ... ...

Abstract	Background: Early studies on transcatheter aortic valve replacement (TAVR) outcomes showed that female sex was associated with better survival. With increased use of new-generation valves, the impact of sex on contemporary TAVR outcomes is less well known. Methods: Retrospective analysis using institutional National Cardiovascular Data Registry STS/ACC TVT data was performed on all patients undergoing TAVR at Yale New Haven Hospital from July 2012 to August 2019. New-generation valves were Evolut PRO, Evolut R, and SAPIEN 3. Old-generation valves were CoreValve, SAPIEN, and SAPIEN XT. Log-rank test and Kaplan-Meier curves were used to compare sex differences in survival up to 1 year after TAVR. Cox modeling was used to adjust for baseline and procedural characteristic differences. Results: 927 consecutive patients (41.4% women) underwent TAVR. Women were older (82.8 vs 80.6 years old; p < 0.001) with higher STS mortality scores compared with men (7.6% vs 6.4%; p < 0.001) despite lower prevalence of cardiovascular comorbidities including coronary artery disease, peripheral artery disease, and smoking. Most cases used transfemoral access (90.5%) and new-generation devices (72.3%). Women received smaller valves compared with men (20-26 mm: 78.0% vs 32.9%; 29-34 mm: 22.1% vs 67.1%; overall p < 0.0001). There were no statistically significant differences between sexes in both unadjusted and adjusted 1-year mortality. Conclusion: Our data show no significant difference in 1-year survival between sexes using primarily new generation valves. Further studies should reassess the impact of sex on TAVR outcomes and whether newer technologies like new valve design and sizes, and CT imaging may have eliminated sex-based disparities.
MeSH term(s)	Humans ; Female ; Male ; Aged, 80 and over ; Transcatheter Aortic Valve Replacement ; Aortic Valve/diagnostic imaging ; Aortic Valve/surgery ; Aortic Valve Stenosis/diagnosis ; Aortic Valve Stenosis/surgery ; Retrospective Studies ; Treatment Outcome ; Heart Valve Prosthesis ; Prosthesis Design ; Risk Factors
Language	English
Publishing date	2023-12-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	779519-1
ISSN	1874-1754 ; 0167-5273
ISSN (online)	1874-1754
ISSN	0167-5273
DOI	10.1016/j.ijcard.2023.131643
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Zs.A 1673: Show issues

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Article ; Online: Impact of New-Onset Conduction Disturbances following Transcatheter Aortic Valve Replacement on Outcomes: A Single-Center Study.

See, Claudia / Wang, Yanting / Huang, Haocheng / Parise, Helen / Yang, Yiping / Tirziu, Daniela / Francese, Dominic P / Papoutsidakis, Nikolaos / Bader, Eric / Kaple, Ryan K / Cleman, Michael / Lansky, Alexandra J / Forrest, John K

Journal of interventional cardiology

2023 Volume 2023, Page(s) 5390338

Abstract: Background: Transcatheter aortic valve replacement (TAVR) is known to increase the incidence of conduction disturbances compared to surgical aortic valve replacement; however, there are limited data on the impact and duration of these conduction ... ...

Abstract	Background: Transcatheter aortic valve replacement (TAVR) is known to increase the incidence of conduction disturbances compared to surgical aortic valve replacement; however, there are limited data on the impact and duration of these conduction disturbances on longer term outcomes. Objective: To determine the differential impact of persistent versus nonpersistent new-onset conduction disturbances on TAVR-related complications and outcomes. Methods: This is a single-center retrospective analysis of 927 consecutive patients with aortic stenosis who underwent TAVR at Yale New Haven Hospital from July 2012 to August 2019. Patients with new-onset conduction disturbances within 7 days following TAVR were selected for this study. Persistent and nonpersistent disturbances were, respectively, defined as persisting or not persisting on all patient ECGs for up to 1.5 years after TAVR or until death. Results: Within 7 days after TAVR, conduction disturbances occurred in 42.3% (392/927) of the patients. Conduction disturbances persisted in 150 (38%) patients and did not persist in 187 (48%) patients, and 55 (14%) patients were excluded for having mixed (both persistent and nonpersistent) disturbances. Compared with nonpersistent disturbances, patients with persistent disturbances were more likely to receive a PPM within 7 days after the TAVR procedure (46.0% versus 4.3%, Conclusion: Persistent conduction disturbances were associated with a greater cardiac and all-cause mortality rate at one year following TAVR. Future research should investigate periprocedural factors to reduce persistent conduction disturbances and outcomes beyond one year follow-up.
MeSH term(s)	Humans ; Transcatheter Aortic Valve Replacement/adverse effects ; Retrospective Studies ; Treatment Outcome ; Aortic Valve/surgery ; Aortic Valve Stenosis/surgery ; Risk Factors
Language	English
Publishing date	2023-05-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	1036325-7
ISSN	1540-8183 ; 0896-4327
ISSN (online)	1540-8183
ISSN	0896-4327
DOI	10.1155/2023/5390338
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Zs.A 2719: Show issues

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Article ; Online: Percutaneous Transluminal Angioplasty for Infrapopliteal Chronic Limb-Threatening Ischemia: A Systematic Review and Meta-analysis of Primary Patency and Binary Restenosis Rates.

Snyder, Daniel J / Zilinyi, Robert S / Pruthi, Sonal / George, Sareena / Tirziu, Daniela / Lansky, Alexandra / Mintz, Ari J / Sethi, Sanjum S / Parikh, Sahil A

Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists

2023 , Page(s) 15266028231212133

Abstract: Purpose: Percutaneous transluminal angioplasty (PTA) remains the mainstay of endovascular therapy for infrapopliteal chronic limb-threatening ischemia (CLTI), but outcomes have not been well characterized using high-quality data. The aim of this meta- ... ...

Abstract	Purpose: Percutaneous transluminal angioplasty (PTA) remains the mainstay of endovascular therapy for infrapopliteal chronic limb-threatening ischemia (CLTI), but outcomes have not been well characterized using high-quality data. The aim of this meta-analysis was to provide an updated benchmark for rates of primary patency and binary restenosis after PTA using prospectively collected, predominantly core-lab adjudicated randomized controlled trial (RCT) data. Materials and methods: MEDLINE, EMBASE, Cochrane Central, and ClinicalTrials.gov were queried for RCTs published through November 2022 using PTA as a control arm and including patients with infrapopliteal CLTI. Studies were excluded if >25% of patients had intermittent claudication, other vessels were included, or primary patency or binary restenosis were not outcomes. Outcomes were analyzed using random effects models. This analysis was publicly registered (PROSPERO ID#394543). No funding was utilized. Results: Seventeen RCTs were included (1048 patients, 1279 lesions). Pooled primary patency rates using data from 6 RCTs were 68% at 6 months (95% confidence interval [CI]=45%-84%) and 66% at 12 months (95% CI=51%-79%). Pooled binary restenosis rates using data from 11 RCTs were 54% at 6 months (95% CI=33%-73%) and 60% at 9 to 12 months (95% CI=39%-78%). Significant heterogeneity was present in all outcomes ( Conclusions: This meta-analysis provides estimates for binary restenosis and primary patency following PTA utilizing prospectively collected, predominantly core-lab adjudicated data. Results demonstrate 1-year primary patency rates that are 10% to 20% higher than what has been historically used in power calculations. These new estimates will help facilitate more accurate power analysis for future RCTs. Clinical impact: Rates of primary patency and binary restenosis after percutaneous transluminal angioplasty (PTA) have not been well-described using high-quality data, and investigators have been utilizing estimates of 40% to 50% and 45% to 65%, respectively, when performing power calculations for trials. This meta-analysis demonstrates using high-quality, prospectively collected, and predominantly core-lab adjudicated randomized controlled trial data that actual rates of primary patency are closer to 60% up to 1 year following PTA and provides the first meta-analysis estimate of binary restenosis rates up to 1 year after PTA. These estimates will help facilitate more accurate power calculations for future RCTs in this space.
Language	English
Publishing date	2023-11-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2006618-1
ISSN	1545-1550 ; 1526-6028
ISSN (online)	1545-1550
ISSN	1526-6028
DOI	10.1177/15266028231212133
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