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Article ; Online: Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.

Song, Yingying / Shou, Shuyu / Guo, Huimin / Gao, Zixiang / Liu, Nannan / Yang, Yang / Wang, Feifei / Deng, Qiang / Liu, Jing / Xie, Youhua

Virologica Sinica

2022 Volume 37, Issue 4, Page(s) 558–568

Abstract: Hepatitis B virus (HBV) is a primary cause of chronic liver diseases in humans. HBV infection exhibits strict host and tissue tropism. HBV core promoter (Cp) drives transcription of pregenomic RNA (pgRNA) and plays a key role in the viral life cycle. ... ...

Abstract	Hepatitis B virus (HBV) is a primary cause of chronic liver diseases in humans. HBV infection exhibits strict host and tissue tropism. HBV core promoter (Cp) drives transcription of pregenomic RNA (pgRNA) and plays a key role in the viral life cycle. Hepatocyte nuclear factor 4α (HNF4α) acts as a major transcriptional factor that stimulates Cp. In this work, we reported that BEL7404 cell line displayed a high efficiency of DNA transfection and high levels of HBV antigen expression after transfection of HBV replicons without prominent viral replication. The introduction of exogenous HNF4α and human sodium taurocholate cotransporting polypeptide (hNTCP) expression into BEL7404 made it permissive for HBV replication and susceptible to HBV infection. BEL7404-derived cell lines with induced HBV permissiveness and susceptibility were constructed by stable co-transfection of hNTCP and Tet-inducible HNF4α followed by limiting dilution cloning. HBV replication in such cells was sensitive to inhibition by nucleotide analog tenofovir, while the infection was inhibited by HBV entry inhibitors. This cell culture system provides a new and additional tool for the study of HBV replication and infection as well as the characterization of antiviral agents.
MeSH term(s)	Antiviral Agents/therapeutic use ; Cell Culture Techniques ; Hepatitis B ; Hepatitis B virus/physiology ; Hepatocytes ; Humans ; Virus Replication
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2022-05-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1011219-4
ISSN	1995-820X ; 1000-3223 ; 1003-5125
ISSN (online)	1995-820X
ISSN	1000-3223 ; 1003-5125
DOI	10.1016/j.virs.2022.05.002
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Article: Animal Models for COVID-19: Hamsters, Mouse, Ferret, Mink, Tree Shrew, and Non-human Primates.

Shou, Shuyu / Liu, Menghui / Yang, Yang / Kang, Ning / Song, Yingying / Tan, Dan / Liu, Nannan / Wang, Feifei / Liu, Jing / Xie, Youhua

Frontiers in microbiology

2021 Volume 12, Page(s) 626553

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus causing acute respiratory tract infection in humans. The virus has the characteristics of rapid transmission, long incubation period and strong pathogenicity, and has ... ...

Abstract	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus causing acute respiratory tract infection in humans. The virus has the characteristics of rapid transmission, long incubation period and strong pathogenicity, and has spread all over the world. Therefore, it is of great significance to select appropriate animal models for antiviral drug development and therapeutic effect evaluation. Here, we review and compare the current animal models of SARS-CoV-2.
Language	English
Publishing date	2021-08-31
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2021.626553
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Article ; Online: Bioaccumulation of BDE47 in testes by TiO

Wang, Chao / Zhu, Jiansheng / Gong, Xing / Liang, Yinyin / Xu, Shuyu / Yu, Yongquan / Yang, Liu / Xu, Jiayi / Wang, Shou-Lin

Nanotoxicology

2021 Volume 15, Issue 8, Page(s) 1073–1086

Abstract: This study attempts to explore the potential impact of titanium dioxide nanoparticles (n- ... ...

Abstract	This study attempts to explore the potential impact of titanium dioxide nanoparticles (n-TiO
MeSH term(s)	Animals ; Bioaccumulation ; Intercellular Junctions ; Male ; Nanoparticles/toxicity ; Testis ; Titanium/toxicity ; Water Pollutants, Chemical/toxicity ; Zebrafish
Chemical Substances	Water Pollutants, Chemical ; titanium dioxide (15FIX9V2JP) ; Titanium (D1JT611TNE)
Language	English
Publishing date	2021-08-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2237988-5
ISSN	1743-5404 ; 1743-5390
ISSN (online)	1743-5404
ISSN	1743-5390
DOI	10.1080/17435390.2021.1966538
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Article ; Online: Complete mitochondrial genome of Pallas's Leaf Warbler (

Jiao, Shu-Yu / Liu, Zheng-Xi / Yu, Feng / Yao, Ji-Yuan / Li, Yu-Mei / Yan, Shou-Qing

Mitochondrial DNA. Part B, Resources

2018 Volume 3, Issue 1, Page(s) 211–212

Abstract: In the present study, the complete mitochondrial DNA sequence of Pallas's Leaf Warbler ( ...

Abstract	In the present study, the complete mitochondrial DNA sequence of Pallas's Leaf Warbler (
Language	English
Publishing date	2018-02-12
Publishing country	England
Document type	Journal Article
ISSN	2380-2359
ISSN (online)	2380-2359
DOI	10.1080/23802359.2017.1403867
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Article ; Online: Comparative analysis and integrative classification of NCI60 cell lines and primary tumors using gene expression profiling data.

Wang, Huixia / Huang, Shuguang / Shou, Jianyong / Su, Eric W / Onyia, Jude E / Liao, Birong / Li, Shuyu

BMC genomics

2006 Volume 7, Page(s) 166

Abstract: Background: NCI60 cell lines are derived from cancers of 9 tissue origins and have been invaluable in vitro models for cancer research and anti-cancer drug screen. Although extensive studies have been carried out to assess the molecular features of ... ...

Abstract	Background: NCI60 cell lines are derived from cancers of 9 tissue origins and have been invaluable in vitro models for cancer research and anti-cancer drug screen. Although extensive studies have been carried out to assess the molecular features of NCI60 cell lines related to cancer and their sensitivities to more than 100,000 chemical compounds, it remains unclear if and how well these cell lines represent or model their tumor tissues of origin. Identification and confirmation of correct origins of NCI60 cell lines are critical to their usage as model systems and to translate in vitro studies into clinical potentials. Here we report a direct comparison between NCI60 cell lines and primary tumors by analyzing global gene expression profiles. Results: Comparative analysis suggested that 51 of 59 cell lines we analyzed represent their presumed tumors of origin. Taking advantage of available clinical information of primary tumor samples used to generate gene expression profiling data, we further classified those cell lines with the correct origins into different subtypes of cancer or different stages in cancer development. For example, 6 of 7 non-small cell lung cancer cell lines were classified as lung adenocarcinomas and all of them were classified into late stages in tumor progression. Conclusion: Taken together, we developed and applied a novel approach for systematic comparative analysis and integrative classification of NCI60 cell lines and primary tumors. Our results could provide guidance to the selection of appropriate cell lines for cancer research and pharmaceutical compound screenings. Moreover, this gene expression profile based approach can be generally applied to evaluate experimental model systems such as cell lines and animal models for human diseases.
MeSH term(s)	Carcinoma, Non-Small-Cell Lung/classification ; Carcinoma, Non-Small-Cell Lung/genetics ; Cell Line, Tumor ; Central Nervous System Neoplasms/classification ; Central Nervous System Neoplasms/genetics ; Gene Expression Regulation ; Gene Expression Regulation, Neoplastic ; Humans ; Leukemia/classification ; Leukemia/genetics ; Lung Neoplasms/classification ; Lung Neoplasms/genetics
Language	English
Publishing date	2006-07-03
Publishing country	England
Document type	Comparative Study ; Journal Article
ISSN	1471-2164
ISSN (online)	1471-2164
DOI	10.1186/1471-2164-7-166
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Article ; Online: Comparative analysis and integrative classification of NCI60 cell lines and primary tumors using gene expression profiling data

Onyia Jude E / Su Eric W / Shou Jianyong / Huang Shuguang / Wang Huixia / Liao Birong / Li Shuyu

BMC Genomics, Vol 7, Iss 1, p

2006 Volume 166

Abstract: Abstract Background NCI60 cell lines are derived from cancers of 9 tissue origins and have been invaluable in vitro models for cancer research and anti-cancer drug screen. Although extensive studies have been carried out to assess the molecular features ... ...

Abstract	Abstract Background NCI60 cell lines are derived from cancers of 9 tissue origins and have been invaluable in vitro models for cancer research and anti-cancer drug screen. Although extensive studies have been carried out to assess the molecular features of NCI60 cell lines related to cancer and their sensitivities to more than 100,000 chemical compounds, it remains unclear if and how well these cell lines represent or model their tumor tissues of origin. Identification and confirmation of correct origins of NCI60 cell lines are critical to their usage as model systems and to translate in vitro studies into clinical potentials. Here we report a direct comparison between NCI60 cell lines and primary tumors by analyzing global gene expression profiles. Results Comparative analysis suggested that 51 of 59 cell lines we analyzed represent their presumed tumors of origin. Taking advantage of available clinical information of primary tumor samples used to generate gene expression profiling data, we further classified those cell lines with the correct origins into different subtypes of cancer or different stages in cancer development. For example, 6 of 7 non-small cell lung cancer cell lines were classified as lung adenocarcinomas and all of them were classified into late stages in tumor progression. Conclusion Taken together, we developed and applied a novel approach for systematic comparative analysis and integrative classification of NCI60 cell lines and primary tumors. Our results could provide guidance to the selection of appropriate cell lines for cancer research and pharmaceutical compound screenings. Moreover, this gene expression profile based approach can be generally applied to evaluate experimental model systems such as cell lines and animal models for human diseases.
Keywords	Genetics ; QH426-470 ; Biology (General) ; QH301-705.5 ; Science ; Q ; DOAJ:Genetics ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Biotechnology ; TP248.13-248.65
Subject code	616
Language	English
Publishing date	2006-07-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The effects of proton pump inhibitor on hepatic vascular responsiveness and hemodynamics in cirrhotic rats.

Hsin, I-Fang / Hsu, Shao-Jung / Chuang, Chiao-Lin / Huo, Teh-Ia / Huang, Hui-Chun / Lee, Fa-Yauh / Ho, Hsin-Ling / Chang, Shu-Yu / Lee, Shou-Dong

Journal of the Chinese Medical Association : JCMA

2018 Volume 81, Issue 7, Page(s) 585–592

Abstract: Background: Liver cirrhosis is associated with increased intrahepatic resistance due to hepatic fibrosis and exaggerated vasoconstriction. Recent studies have indicated that proton pump inhibitors (PPIs), in addition to acid suppression, modulate ... ...

Abstract	Background: Liver cirrhosis is associated with increased intrahepatic resistance due to hepatic fibrosis and exaggerated vasoconstriction. Recent studies have indicated that proton pump inhibitors (PPIs), in addition to acid suppression, modulate vasoactive substances and vasoresponsiveness. PPIs are frequently prescribed in patients with cirrhosis due to a higher prevalence of peptic ulcers, however other impacts are unknown. Methods: Liver cirrhosis was induced in Sprague-Dawley rats with common bile duct ligation (BDL). On the 29th day after BDL and after hemodynamic measurements, the intrahepatic vascular responsiveness to high concentrations of endothelin-1 (ET-1) was evaluated after preincubation with (1) Krebs solution (vehicle), (2) esomeprazole (30 μM), or (3) esomeprazole plus N Results: Esomeprazole did not affect hepatic ET-1 vasoresponsiveness. The hepatic protein expressions of the aforementioned factors were not significantly different among the groups. There were no significant differences in hemodynamics, liver biochemistry and hepatic fibrosis after chronic esomeprazole administration. Conclusion: PPIs did not affect hepatic vasoresponsiveness or the release of vasoactive substances. Furthermore, they did not influence hemodynamics, liver biochemistry or severity of hepatic fibrosis in the cirrhotic rats.
MeSH term(s)	Animals ; Endothelin-1/pharmacology ; Esomeprazole/pharmacology ; Hemodynamics/drug effects ; Hypertension, Portal/physiopathology ; Liver Cirrhosis, Experimental/drug therapy ; Liver Cirrhosis, Experimental/physiopathology ; Male ; Proton Pump Inhibitors/pharmacology ; Proton Pump Inhibitors/therapeutic use ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Endothelin-1 ; Proton Pump Inhibitors ; Esomeprazole (N3PA6559FT)
Language	English
Publishing date	2018-05-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2107283-8
ISSN	1728-7731 ; 1726-4901
ISSN (online)	1728-7731
ISSN	1726-4901
DOI	10.1016/j.jcma.2018.01.011
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Article: [Study on the effect of cold air over the coagulation function by exposing healthy rats and hypertensive rats to a simulated cold air].

Luo, Bin / Zhang, Shu-Yu / Zhou, Ji / Ma, Shou-Cun / Wang, Bao-Jian

Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology

2012 Volume 28, Issue 5, Page(s) 390–393

Abstract: Objective: To explore the effect of temperature dropping process in cold air on the coagulation function both in healthy and hypertensive rats.: Methods: Twenty-four male healthy Wistar rats and 24 male spontaneous hypertensive rats (SHR) were ... ...

Abstract	Objective: To explore the effect of temperature dropping process in cold air on the coagulation function both in healthy and hypertensive rats. Methods: Twenty-four male healthy Wistar rats and 24 male spontaneous hypertensive rats (SHR) were randomly divided into the minimum temperature group (Tim), Tmin control group (Tmin-c), recovery temperature group (Tr) and Tr control group (Tr-c), With the simulated temperature dropping process of a cold air, collected from Zhangye city in March of 2011, the groups of Tmin and Tr were exposed to this process. Both at the Tmin and Tr, blood were collected from the rats for coagulation function measurements. Results: Compared with the control group, no significant difference was found in the results of activated partial thrombin time(APIT), pro-thrombin time (PF) and thrombin time (TT) between any groups in any strains (P > 0.05). The fibrinogen (Fbg) and fibrinogen-time were found to be obvious higher and shorter in Tmin and Tr of healthy rats and in Tmin of hypertensive rats in contrast to the control group. Hypertensive rats had higher level of fibrinogen and shorter level of fibrinogen-time. Conclusion: The temperature dropping process induced the increase of plasma Fbg both in the healthy and hypertensive subjects, which might be the reason to explain the higher occurrence of cardiovascular diseases event especially these activated through the formation of thrombin during cold air stress. Besides, the coagulation function of healthy subjects was more likely to be affected by cold air than the hypertensive subjects.
MeSH term(s)	Animals ; Blood Coagulation ; Cold Temperature ; Hypertension/physiopathology ; Male ; Rats ; Rats, Inbred SHR ; Rats, Wistar ; Thrombin Time
Language	Chinese
Publishing date	2012-09
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1000-6834
ISSN	1000-6834
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Article ; Online: Cdc7-Dbf4-mediated phosphorylation of HSP90-S164 stabilizes HSP90-HCLK2-MRN complex to enhance ATR/ATM signaling that overcomes replication stress in cancer

An Ning Cheng / Chi-Chen Fan / Yu-Kang Lo / Cheng-Liang Kuo / Hui-Chun Wang / I.-Hsin Lien / Shu-Yu Lin / Chung-Hsing Chen / Shih Sheng Jiang / I.-Shou Chang / Hsueh-Fen Juan / Ping-Chiang Lyu / Alan Yueh-Luen Lee

Scientific Reports, Vol 7, Iss 1, Pp 1-

2017 Volume 15

Abstract: Abstract Cdc7-Dbf4 kinase plays a key role in the initiation of DNA replication and contributes to the replication stress in cancer. The activity of human Cdc7-Dbf4 kinase remains active and acts as an effector of checkpoint under replication stress. ... ...

Abstract	Abstract Cdc7-Dbf4 kinase plays a key role in the initiation of DNA replication and contributes to the replication stress in cancer. The activity of human Cdc7-Dbf4 kinase remains active and acts as an effector of checkpoint under replication stress. However, the downstream targets of Cdc7-Dbf4 contributed to checkpoint regulation and replication stress-support function in cancer are not fully identified. In this work, we showed that aberrant Cdc7-Dbf4 induces DNA lesions that activate ATM/ATR-mediated checkpoint and homologous recombination (HR) DNA repair. Using a phosphoproteome approach, we identified HSP90-S164 as a target of Cdc7-Dbf4 in vitro and in vivo. The phosphorylation of HSP90-S164 by Cdc7-Dbf4 is required for the stability of HSP90-HCLK2-MRN complex and the function of ATM/ATR signaling cascade and HR DNA repair. In clinically, the phosphorylation of HSP90-S164 indeed is increased in oral cancer patients. Our results indicate that aberrant Cdc7-Dbf4 enhances replication stress tolerance by rewiring ATR/ATM mediated HR repair through HSP90-S164 phosphorylation and by promoting recovery from replication stress. We provide a new solution to a subtyping of cancer patients with dominant ATR/HSP90 expression by combining inhibitors of ATR-Chk1, HSP90, or Cdc7 in cancer combination therapy.
Keywords	Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2017-12-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: [Research progress in molecular biology of important proteins of Clonorchis sinensis].

Jiang, Shou-fu / Zhang, Shu-yu / Cai, Li

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases

2003 Volume 21, Issue 1, Page(s) 50–54

MeSH term(s)	Animals ; Antigens, Helminth/genetics ; Clonorchis sinensis/genetics ; Cysteine Endopeptidases/genetics ; Glutathione Transferase/genetics ; Helminth Proteins/genetics ; Phosphoglycerate Kinase/genetics ; Tropomyosin/genetics
Chemical Substances	Antigens, Helminth ; GRCSP protein, Clonorchis sinensis ; Helminth Proteins ; Tropomyosin ; Glutathione Transferase (EC 2.5.1.18) ; Phosphoglycerate Kinase (EC 2.7.2.3) ; Cysteine Endopeptidases (EC 3.4.22.-)
Language	Chinese
Publishing date	2003
Publishing country	China
Document type	Journal Article ; Review
ZDB-ID	639272-6
ISSN	1000-7423
ISSN	1000-7423
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