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Article ; Online: Mapping Neurotransmitter Identity in the Whole-Mount

Meissner, Geoffrey W / Nern, Aljoscha / Singer, Robert H / Wong, Allan M / Malkesman, Oz / Long, Xi

2018 Volume 211, Issue 2, Page(s) 473–482

Abstract: Identifying the neurotransmitters used by specific neurons is a critical step in understanding the function of neural circuits. However, methods for the consistent and efficient detection of neurotransmitter markers remain limited. ... ...

Abstract	Identifying the neurotransmitters used by specific neurons is a critical step in understanding the function of neural circuits. However, methods for the consistent and efficient detection of neurotransmitter markers remain limited. Fluorescence
MeSH term(s)	Animals ; Brain/metabolism ; Brain Chemistry ; Drosophila/metabolism ; In Situ Hybridization, Fluorescence/methods ; Neurotransmitter Agents/analysis ; Neurotransmitter Agents/metabolism
Chemical Substances	Neurotransmitter Agents
Language	English
Publishing date	2018-12-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2167-2
ISSN	1943-2631 ; 0016-6731
ISSN (online)	1943-2631
ISSN	0016-6731
DOI	10.1534/genetics.118.301749
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Article ; Online: A searchable image resource of

Meissner, Geoffrey W / Nern, Aljoscha / Dorman, Zachary / DePasquale, Gina M / Forster, Kaitlyn / Gibney, Theresa / Hausenfluck, Joanna H / He, Yisheng / Iyer, Nirmala A / Jeter, Jennifer / Johnson, Lauren / Johnston, Rebecca M / Lee, Kelley / Melton, Brian / Yarbrough, Brianna / Zugates, Christopher T / Clements, Jody / Goina, Cristian / Otsuna, Hideo /

Rokicki, Konrad / Svirskas, Robert R / Aso, Yoshinori / Card, Gwyneth M / Dickson, Barry J / Ehrhardt, Erica / Goldammer, Jens / Ito, Masayoshi / Kainmueller, Dagmar / Korff, Wyatt / Mais, Lisa / Minegishi, Ryo / Namiki, Shigehiro / Rubin, Gerald M / Sterne, Gabriella R / Wolff, Tanya / Malkesman, Oz

eLife

2023 Volume 12

Abstract: Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage ... ...

Abstract	Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of
MeSH term(s)	Animals ; Drosophila/metabolism ; Neurons/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Central Nervous System/metabolism ; Neurosciences ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	Drosophila Proteins ; GAL4 protein, Drosophila ; Transcription Factors
Language	English
Publishing date	2023-02-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.80660
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: An unbiased template of the Drosophila brain and ventral nerve cord.

Bogovic, John A / Otsuna, Hideo / Heinrich, Larissa / Ito, Masayoshi / Jeter, Jennifer / Meissner, Geoffrey / Nern, Aljoscha / Colonell, Jennifer / Malkesman, Oz / Ito, Kei / Saalfeld, Stephan

PloS one

2020 Volume 15, Issue 12, Page(s) e0236495

Abstract: The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes. Brain templates are essential for comparative biological studies ... ...

Abstract	The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable analyzing many individuals in a common reference space. Several central brain templates exist for Drosophila, but every one is either biased, uses sub-optimal tissue preparation, is imaged at low resolution, or does not account for artifacts. No publicly available Drosophila ventral nerve cord template currently exists. In this work, we created high-resolution templates of the Drosophila brain and ventral nerve cord using the best-available technologies for imaging, artifact correction, stitching, and template construction using groupwise registration. We evaluated our central brain template against the four most competitive, publicly available brain templates and demonstrate that ours enables more accurate registration with fewer local deformations in shorter time.
MeSH term(s)	Animals ; Brain/anatomy & histology ; Brain/ultrastructure ; Drosophila melanogaster/anatomy & histology ; Drosophila melanogaster/ultrastructure ; Female ; Image Processing, Computer-Assisted/statistics & numerical data ; Male ; Microscopy, Confocal ; Microscopy, Electron ; Nerve Tissue/anatomy & histology ; Nerve Tissue/ultrastructure ; Neurons/ultrastructure
Language	English
Publishing date	2020-12-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0236495
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Traumatic brain injury - modeling neuropsychiatric symptoms in rodents.

Malkesman, Oz / Tucker, Laura B / Ozl, Jessica / McCabe, Joseph T

Frontiers in neurology

2013 Volume 4, Page(s) 157

Abstract: Each year in the US, ∼1.5 million people sustain a traumatic brain injury (TBI). Victims of TBI can suffer from chronic post-TBI symptoms, such as sensory and motor deficits, cognitive impairments including problems with memory, learning, and attention, ... ...

Abstract	Each year in the US, ∼1.5 million people sustain a traumatic brain injury (TBI). Victims of TBI can suffer from chronic post-TBI symptoms, such as sensory and motor deficits, cognitive impairments including problems with memory, learning, and attention, and neuropsychiatric symptoms such as depression, anxiety, irritability, aggression, and suicidal rumination. Although partially associated with the site and severity of injury, the biological mechanisms associated with many of these symptoms - and why some patients experience differing assortments of persistent maladies - are largely unknown. The use of animal models is a promising strategy for elucidation of the mechanisms of impairment and treatment, and learning, memory, sensory, and motor tests have widespread utility in rodent models of TBI and psychopharmacology. Comparatively, behavioral tests for the evaluation of neuropsychiatric symptomatology are rarely employed in animal models of TBI and, as determined in this review, the results have been inconsistent. Animal behavioral studies contribute to the understanding of the biological mechanisms by which TBI is associated with neurobehavioral symptoms and offer a powerful means for pre-clinical treatment validation. Therefore, further exploration of the utility of animal behavioral tests for the study of injury mechanisms and therapeutic strategies for the alleviation of emotional symptoms are relevant and essential.
Language	English
Publishing date	2013-10-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2564214-5
ISSN	1664-2295
ISSN	1664-2295
DOI	10.3389/fneur.2013.00157
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Article ; Online: An unbiased template of the Drosophila brain and ventral nerve cord.

John A Bogovic / Hideo Otsuna / Larissa Heinrich / Masayoshi Ito / Jennifer Jeter / Geoffrey Meissner / Aljoscha Nern / Jennifer Colonell / Oz Malkesman / Kei Ito / Stephan Saalfeld

PLoS ONE, Vol 15, Iss 12, p e

2020 Volume 0236495

Abstract	The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable analyzing many individuals in a common reference space. Several central brain templates exist for Drosophila, but every one is either biased, uses sub-optimal tissue preparation, is imaged at low resolution, or does not account for artifacts. No publicly available Drosophila ventral nerve cord template currently exists. In this work, we created high-resolution templates of the Drosophila brain and ventral nerve cord using the best-available technologies for imaging, artifact correction, stitching, and template construction using groupwise registration. We evaluated our central brain template against the four most competitive, publicly available brain templates and demonstrate that ours enables more accurate registration with fewer local deformations in shorter time.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons.

Meissner, Geoffrey W / Grimm, Jonathan B / Johnston, Rebecca M / Sutcliffe, Ben / Ng, Julian / Jefferis, Gregory S X E / Cachero, Sebastian / Lavis, Luke D / Malkesman, Oz

PloS one

2018 Volume 13, Issue 8, Page(s) e0200759

Abstract: The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, ...

Abstract	The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. We found that tissue clearing and mounting in DPX substantially improves signal quality when combined with specific non-cyanine fluorophores. We compared and combined this labeling technique with standard immunohistochemistry in the Drosophila brain.
MeSH term(s)	Animals ; Animals, Genetically Modified ; Brain/cytology ; Drosophila melanogaster/cytology ; Female ; Fluorescent Dyes/chemical synthesis ; Fluorescent Dyes/chemistry ; Immunohistochemistry ; Microscopy, Confocal ; Molecular Structure ; Neurons/cytology ; Staining and Labeling
Chemical Substances	Fluorescent Dyes
Language	English
Publishing date	2018-08-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0200759
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Article ; Online: Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons.

Geoffrey W Meissner / Jonathan B Grimm / Rebecca M Johnston / Ben Sutcliffe / Julian Ng / Gregory S X E Jefferis / Sebastian Cachero / Luke D Lavis / Oz Malkesman

PLoS ONE, Vol 13, Iss 8, p e

2018 Volume 0200759

Abstract	The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. We found that tissue clearing and mounting in DPX substantially improves signal quality when combined with specific non-cyanine fluorophores. We compared and combined this labeling technique with standard immunohistochemistry in the Drosophila brain.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Reverse translational strategies for developing animal models of bipolar disorder.

Malkesman, Oz / Austin, Daniel R / Chen, Guang / Manji, Husseini K

Disease models & mechanisms

2009 Volume 2, Issue 5-6, Page(s) 238–245

Abstract: Bipolar disorder (BD) affects a significant portion of the population of the world, yet there has been limited success in developing novel treatments for the disorder. One of the major reasons for this dearth is the absence of suitable animal models for ... ...

Abstract	Bipolar disorder (BD) affects a significant portion of the population of the world, yet there has been limited success in developing novel treatments for the disorder. One of the major reasons for this dearth is the absence of suitable animal models for BD. Traditionally, animal models of human phenomena have been evaluated based on similarity to the human syndrome, response to appropriately corresponding medications, and the degree to which a model supports a common mechanistic theory between the human disorder and the model itself. The following review emphasizes the use of 'reverse translation', drawing on patient-based findings to develop suitable animal models for BD. We highlight some examples of this strategy, emphasizing their construct validity as a starting point. These studies have produced informative models that have altered the expression of genes/pathways implicated in BD, including the point mutation D181A of mouse mitochondrial DNA polymerase (POLG), glutamate receptor 6 (GluR6), Clock, extracellular regulated kinase 1 (ERK1), glycogen synthase kinase-3beta (GSK-3beta), B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated athanogene (BAG-1). These studies demonstrate that this method is useful, viable and deserves attention in new efforts to generate animal models of BD.
MeSH term(s)	Animals ; Bipolar Disorder/complications ; Bipolar Disorder/pathology ; Depression/complications ; Disease Models, Animal ; Disease Susceptibility ; Humans ; Mutation/genetics
Language	English
Publishing date	2009-04-30
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Intramural ; Review
ZDB-ID	2451104-3
ISSN	1754-8411 ; 1754-8403
ISSN (online)	1754-8411
ISSN	1754-8403
DOI	10.1242/dmm.001628
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Article: Mother-Infant Interactions in Rats Lacking CCK(A) Receptors.

Lavi-Avnon, Yael / Malkesman, Oz / Hurwitz, Itay / Weller, Aron

Behavioral neuroscience

2004 Volume 118, Issue 2, Page(s) 282–289

Abstract: Mediation of mother-infant interactions by the brain-gut peptide cholecystokinin (CCK) was examined by observing behavior of Otsuka Long-Evans Tokushima Fatty (OLETF) rats, which lack functional CCKA receptors because of a genetic abnormality. OLETF (n = ...

Abstract	Mediation of mother-infant interactions by the brain-gut peptide cholecystokinin (CCK) was examined by observing behavior of Otsuka Long-Evans Tokushima Fatty (OLETF) rats, which lack functional CCKA receptors because of a genetic abnormality. OLETF (n = 10) and control (Long-Evans Tokushima Otsuka [LETO] n = 10) dams interacted with 1 pup of each line on Postpartum Days 6-9. OLETF pups received more body and anogenital licking and emitted substantially more ultrasonic vocalizations than LETO pups. OLETF dams carried pups less frequently and showed a nursing position more frequently than LETO dams. No significant Pup X Dam Line interactions or line differences in dams' activity were detected. The results provide convergent validity to previous pharmacological studies implicating CCK mediation of both infant and maternal behavior.
MeSH term(s)	Animals ; Brain/metabolism ; Dopamine/metabolism ; Female ; Maternal Behavior/physiology ; Nucleus Accumbens/metabolism ; Object Attachment ; Pregnancy ; Random Allocation ; Rats ; Receptors, Cholecystokinin/metabolism ; Reinforcement (Psychology)
Chemical Substances	Receptors, Cholecystokinin ; Dopamine (VTD58H1Z2X)
Language	English
Publishing date	2004-04
Publishing country	United States
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	230159-3
ISSN	1939-0084 ; 0735-7044
ISSN (online)	1939-0084
ISSN	0735-7044
DOI	10.1037/0735-7044.118.2.282
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Article: Gastric preloads of corn oil and mineral oil produce different patterns of increases of c-Fos-like immunoreacitve cells in the brain of 9-12 day-old rats.

Blumberg, Sara / Schroeder, Mariana / Malkesman, Oz / Torregrossa, Ann Marie / Smith, Gerard P / Weller, Aron

Brain research

2007 Volume 1134, Issue 1, Page(s) 140–147

Abstract: Equivolumetric gastric preloads of corn oil and mineral oil administered to rats on postnatal day 12 (P12) inhibited intake equally during a 30-min test of independent ingestion (II), but preloads of corn oil inhibited intake significantly more than ... ...

Abstract	Equivolumetric gastric preloads of corn oil and mineral oil administered to rats on postnatal day 12 (P12) inhibited intake equally during a 30-min test of independent ingestion (II), but preloads of corn oil inhibited intake significantly more than preloads of mineral oil on P15 and P18 [Weller, A., Gispan, I.H., Armony-Sivan, R., Ritter, R.C., Smith, G.P., 1997. Preloads of corn oil inhibit independent ingestion on postnatal day 15 in rats. Physiol. Behav. 62, 871-874]. It is possible that the equivalent inhibition of intake by the oil preloads on P12 resulted from the failure of the preabsorptive sensory properties of the preloads to be discriminated by peripheral or central sensory mechanisms. To investigate this possibility, we administered equivolumetric gastric preloads of 25% corn oil and 25% mineral oil to pups on P9-12 and counted the number of c-Fos-like immunoreactive (CFLI) cells in central sites that are activated by food intake and postingestive preabsortive mechanisms in adult rats and in pups on P10-11. The major result was that preloads of 25% corn oil and 25% mineral oil that produced equivalent inhibition of II intake produced differential increases of CFLI cells in the forebrain and hindbrain. Specifically, preloads of corn oil increased the number of CFLI cells in the caudal Nucleus Tractus Solitarius significantly more than preloads of mineral oil. Furthermore, preloads of corn oil increased the number of CFLI cells in the Paraventricular and Supraoptic nuclei, but preloads of mineral oil did not. This differential pattern of increases of CFLI cells is evidence that the brain discriminates the preabsorptive sensory properties of preloads of corn oil and mineral oil on P9-12.
MeSH term(s)	Aging/physiology ; Animals ; Animals, Newborn ; Brain/drug effects ; Brain/growth & development ; Cholecystokinin/metabolism ; Corn Oil/pharmacology ; Fatty Acids/metabolism ; Fatty Acids/pharmacology ; Female ; Gastrointestinal Tract/drug effects ; Gastrointestinal Tract/innervation ; Gastrointestinal Tract/physiology ; Hypothalamus/drug effects ; Hypothalamus/growth & development ; Immunohistochemistry ; Male ; Mineral Oil/pharmacology ; Neurons/drug effects ; Neurons/metabolism ; Proto-Oncogene Proteins c-fos/drug effects ; Proto-Oncogene Proteins c-fos/metabolism ; Rats ; Rats, Long-Evans ; Satiety Response/drug effects ; Satiety Response/physiology ; Sensory Receptor Cells/drug effects ; Sensory Receptor Cells/growth & development ; Solitary Nucleus/drug effects ; Solitary Nucleus/growth & development ; Vagus Nerve/drug effects ; Vagus Nerve/growth & development ; Visceral Afferents/drug effects ; Visceral Afferents/growth & development
Chemical Substances	Fatty Acids ; Proto-Oncogene Proteins c-fos ; Corn Oil (8001-30-7) ; Mineral Oil (8020-83-5) ; Cholecystokinin (9011-97-6)
Language	English
Publishing date	2007-02-23
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1200-2
ISSN	1872-6240 ; 0006-8993
ISSN (online)	1872-6240
ISSN	0006-8993
DOI	10.1016/j.brainres.2006.11.062
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