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  1. Article ; Online: Animal Transgenesis and Cloning: Combined Development and Future Perspectives.

    Yamashita, Melissa S / Melo, Eduardo O

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2647, Page(s) 121–149

    Abstract: The revolution in animal transgenesis began in 1981 and continues to become more efficient, cheaper, and faster to perform. New genome editing technologies, especially CRISPR-Cas9, are leading to a new era of genetically modified or edited organisms. ... ...

    Abstract The revolution in animal transgenesis began in 1981 and continues to become more efficient, cheaper, and faster to perform. New genome editing technologies, especially CRISPR-Cas9, are leading to a new era of genetically modified or edited organisms. Some researchers advocate this new era as the time of synthetic biology or re-engineering. Nonetheless, we are witnessing advances in high-throughput sequencing, artificial DNA synthesis, and design of artificial genomes at a fast pace. These advances in symbiosis with animal cloning by somatic cell nuclear transfer (SCNT) allow the development of improved livestock, animal models of human disease, and heterologous production of bioproducts for medical applications. In the context of genetic engineering, SCNT remains a useful technology to generate animals from genetically modified cells. This chapter addresses these fast-developing technologies driving this biotechnological revolution and their association with animal cloning technology.
    MeSH term(s) Animals ; Humans ; Animals, Genetically Modified ; Genetic Engineering ; Gene Editing ; Cloning, Organism ; Cloning, Molecular
    Language English
    Publishing date 2023-04-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3064-8_6
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  2. Article ; Online: Effects of Growth and Differentiation Factor 9 and Bone Morphogenetic Protein 15 overexpression on the steroidogenic metabolism in bovine granulosa cells in vitro.

    Paulini, Fernanda / Melo, Eduardo O

    Reproduction in domestic animals = Zuchthygiene

    2021  Volume 56, Issue 6, Page(s) 837–847

    Abstract: Granulosa cells (GCs) play important roles in the regulation of ovarian functions, and in vitro culture is a relevant model for the study of steroidogenesis in ovarian follicles. Thus, growth factors secreted by the oocyte, like Growth and ... ...

    Abstract Granulosa cells (GCs) play important roles in the regulation of ovarian functions, and in vitro culture is a relevant model for the study of steroidogenesis in ovarian follicles. Thus, growth factors secreted by the oocyte, like Growth and Differentiation Factor 9 (GDF9) and Bone Morphogenetic Protein 15 (BMP15), play an important part in the luteinization of granulosa cells. The aim of this work was to express GDF9 and BMP15 genes in bovine GCs in vitro and evaluate their effects on the luteinization process. Samples of culture medium and GCs transfected with GDF9 and BMP15 were obtained for 21 consecutive days to analyse the steroidogenic hormones' concentration (progesterone (P
    MeSH term(s) Animals ; Bone Morphogenetic Protein 15/genetics ; Bone Morphogenetic Protein 15/metabolism ; Cattle ; Cells, Cultured ; Estradiol/metabolism ; Female ; Granulosa Cells ; Growth Differentiation Factor 9/genetics ; Growth Differentiation Factor 9/metabolism ; Phosphoproteins/genetics ; Phosphoproteins/metabolism ; Progesterone/metabolism
    Chemical Substances Bone Morphogenetic Protein 15 ; Growth Differentiation Factor 9 ; Phosphoproteins ; steroidogenic acute regulatory protein ; Progesterone (4G7DS2Q64Y) ; Estradiol (4TI98Z838E)
    Language English
    Publishing date 2021-03-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.13923
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    In stock of ZB MED Bonn / Germany

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  3. Article: Effects of Growth and Differentiation Factor 9 and Bone Morphogenetic Protein 15 overexpression on the steroidogenic metabolism in bovine granulosa cells in vitro

    Paulini, Fernanda / Melo, Eduardo O

    Reproduction in domestic animals. 2021 June, v. 56, no. 6

    2021  

    Abstract: Granulosa cells (GCs) play important roles in the regulation of ovarian functions, and in vitro culture is a relevant model for the study of steroidogenesis in ovarian follicles. Thus, growth factors secreted by the oocyte, like Growth and ... ...

    Abstract Granulosa cells (GCs) play important roles in the regulation of ovarian functions, and in vitro culture is a relevant model for the study of steroidogenesis in ovarian follicles. Thus, growth factors secreted by the oocyte, like Growth and Differentiation Factor 9 (GDF9) and Bone Morphogenetic Protein 15 (BMP15), play an important part in the luteinization of granulosa cells. The aim of this work was to express GDF9 and BMP15 genes in bovine GCs in vitro and evaluate their effects on the luteinization process. Samples of culture medium and GCs transfected with GDF9 and BMP15 were obtained for 21 consecutive days to analyse the steroidogenic hormones' concentration (progesterone (P₄) and estradiol (E₂)) and the expression of STAR, GDF9 and BMP15 and their respective receptors. The results demonstrated an inhibitory effect of GDF9 and BMPF15 on P₄ secretion in bovine GCs cultured in vitro. Moreover, our study demonstrated the entire expression of their respective receptors (TGFBR1, BMPR1B and BMPR2) and the inhibition of the steroidogenic marker, STAR gene. This work sheds light on a novel biological function of BMP15 and GDF9 in bovine GCs physiology, which could elucidate a non‐described biological role for GDF9 and BMP15 in bovine granulosa cells' metabolism.
    Keywords bone morphogenetic proteins ; cattle ; culture media ; estradiol ; genes ; luteinization ; models ; oocytes ; progesterone ; secretion ; steroidogenesis
    Language English
    Dates of publication 2021-06
    Size p. 837-847.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note NAL-AP-2-clean ; JOURNAL ARTICLE
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.13923
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    In stock of ZB MED Bonn / Germany

    Z 2117: Show issues

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  4. Article: Corrigendum to "Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase" Comput Struct Biotechnol J, 20 (2022), 3899-3910.

    Borges, Patrícia T / Silva, Diogo / Silva, Tomás F D / Brissos, Vânia / Cañellas, Marina / Lucas, Maria Fátima / Masgrau, Laura / Melo, Eduardo P / Machuqueiro, Miguel / Frazão, Carlos / Martins, Lígia O

    Computational and structural biotechnology journal

    2023  Volume 22, Page(s) 2

    Abstract: This corrects the article DOI: 10.1016/j.csbj.2022.07.032.]. ...

    Abstract [This corrects the article DOI: 10.1016/j.csbj.2022.07.032.].
    Language English
    Publishing date 2023-01-04
    Publishing country Netherlands
    Document type Published Erratum
    ZDB-ID 2694435-2
    ISSN 2001-0370
    ISSN 2001-0370
    DOI 10.1016/j.csbj.2022.12.048
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  5. Article ; Online: Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms.

    Taborda, André / Frazão, Tomás / Rodrigues, Miguel V / Fernández-Luengo, Xavier / Sancho, Ferran / Lucas, Maria Fátima / Frazão, Carlos / Melo, Eduardo P / Ventura, M Rita / Masgrau, Laura / Borges, Patrícia T / Martins, Lígia O

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 7289

    Abstract: ... reduction of O ...

    Abstract C-glycosides are natural products with important biological activities but are recalcitrant to degradation. Glycoside 3-oxidases (G3Oxs) are recently identified bacterial flavo-oxidases from the glucose-methanol-coline (GMC) superfamily that catalyze the oxidation of C-glycosides with the concomitant reduction of O
    MeSH term(s) Oxidoreductases/metabolism ; Hydrogen Peroxide ; Glycosides/metabolism ; Glucose/metabolism ; Cardiac Glycosides ; Substrate Specificity ; Glycoside Hydrolases/metabolism
    Chemical Substances Oxidoreductases (EC 1.-) ; C-glycoside ; Hydrogen Peroxide (BBX060AN9V) ; Glycosides ; Glucose (IY9XDZ35W2) ; Cardiac Glycosides ; Glycoside Hydrolases (EC 3.2.1.-)
    Language English
    Publishing date 2023-11-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-42000-3
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  6. Article ; Online: Mucin 2 (MUC2) promoter characterization: an overview.

    Yamashita, Melissa Shizue de A / Melo, Eduardo O

    Cell and tissue research

    2018  Volume 374, Issue 3, Page(s) 455–463

    Abstract: Transgenic livestock have been studied with a well-known interest in improving quantitative and qualitative traits. In order to direct heterologous gene expression, it is indispensable to identify and characterize a promoter suitable for directing the ... ...

    Abstract Transgenic livestock have been studied with a well-known interest in improving quantitative and qualitative traits. In order to direct heterologous gene expression, it is indispensable to identify and characterize a promoter suitable for directing the expression of the gene of interest (GOI) in a tissue-specific way. The gastrointestinal tract is a desirable target for gene expression in several mammalian models. Throughout the surface of the intestinal epithelium, there is an intricate polymer network, formed by gel-forming mucins (especially MUC2 and MUC5AC, of which MUC2 is the major one), which plays a protective role due to the formation of a physical, chemical and immunological barrier between the organism and the environment. The characterization of the gel-forming mucins is difficult because of their large size and repetitive DNA sequences and domains. The main mucin in the small and large intestine, mucin 2 (MUC2), is expressed specifically in goblet cells. MUC2 plays an important role in intestinal homeostasis and its disruption is associated with several diseases and carcinomas. This mucin is also an important marker for elucidating mechanisms that regulate differentiation of the secretory cell lineage. This review presents the state of the art of MUC2 promoter structure and functional characterization.
    MeSH term(s) Animals ; Base Sequence ; Humans ; Mucin-2/genetics ; Promoter Regions, Genetic ; Protective Agents/metabolism ; Transcription Factors/metabolism
    Chemical Substances Mucin-2 ; Protective Agents ; Transcription Factors
    Language English
    Publishing date 2018-09-14
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 125067-x
    ISSN 1432-0878 ; 0302-766X
    ISSN (online) 1432-0878
    ISSN 0302-766X
    DOI 10.1007/s00441-018-2916-9
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    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.94: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  7. Article: Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene.

    Garcia-Melo, Luis Fernando / Morales-Rodríguez, Miguel / Madrigal-Bujaidar, Eduardo / Madrigal-Santillán, Eduardo O / Morales-González, José Antonio / Pineda Cruces, Rosa Natali / Campoy Ramírez, Jorge Alfredo / Damian-Matsumura, Pablo / Tellez-Plancarte, Alexandro / Batina, Nikola / Álvarez-González, Isela

    Journal of analytical methods in chemistry

    2022  Volume 2022, Page(s) 6575140

    Abstract: In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well ...

    Abstract In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the
    Language English
    Publishing date 2022-10-17
    Publishing country Egypt
    Document type Journal Article
    ZDB-ID 2654178-6
    ISSN 2090-8873 ; 2090-8865
    ISSN (online) 2090-8873
    ISSN 2090-8865
    DOI 10.1155/2022/6575140
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  8. Article: Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase.

    Borges, Patrícia T / Silva, Diogo / Silva, Tomás F D / Brissos, Vânia / Cañellas, Marina / Lucas, Maria Fátima / Masgrau, Laura / Melo, Eduardo P / Machuqueiro, Miguel / Frazão, Carlos / Martins, Lígia O

    Computational and structural biotechnology journal

    2022  Volume 20, Page(s) 3899–3910

    Abstract: DyP-type peroxidases (DyPs) are microbial enzymes that catalyze the oxidation of a wide range of substrates, including synthetic dyes, lignin-derived compounds, and metals, such as ... ...

    Abstract DyP-type peroxidases (DyPs) are microbial enzymes that catalyze the oxidation of a wide range of substrates, including synthetic dyes, lignin-derived compounds, and metals, such as Mn
    Language English
    Publishing date 2022-07-21
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2694435-2
    ISSN 2001-0370
    ISSN 2001-0370
    DOI 10.1016/j.csbj.2022.07.032
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  9. Article ; Online: Liver transplantation for the treatment of iatrogenic bile duct injury.

    Cirilo Neto, Olival / Moutinho, Luiz Eduardo Rafael / Melo, Paulo Sérgio Vieira DE / Rabêlo, Priscylla Jennie Monteiro / Amorim, Americo Gusmão / Melo, Cláudio Moura Lacerda

    Revista do Colegio Brasileiro de Cirurgioes

    2023  Volume 50, Page(s) e20233565

    MeSH term(s) Humans ; Liver Transplantation ; Bile Duct Diseases ; Bile Ducts/surgery ; Iatrogenic Disease
    Language Portuguese
    Publishing date 2023-07-28
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2223714-8
    ISSN 1809-4546 ; 1809-4546
    ISSN (online) 1809-4546
    ISSN 1809-4546
    DOI 10.1590/0100-6991e-20233565-en
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  10. Article ; Online: Rationally Guided Improvement of NOV1 Dioxygenase for the Conversion of Lignin-Derived Isoeugenol to Vanillin.

    De Simone, Mario / Alvigini, Laura / Alonso-Cotchico, Lur / Brissos, Vânia / Caroli, Jonatan / Lucas, Maria Fátima / Monza, Emanuele / Melo, Eduardo Pinho / Mattevi, Andrea / Martins, Lígia O

    Biochemistry

    2022  Volume 62, Issue 2, Page(s) 419–428

    Abstract: Biocatalysis is a key tool in both green chemistry and biorefinery fields. NOV1 is a dioxygenase that catalyzes the one-step, coenzyme-free oxidation of isoeugenol into vanillin and holds enormous biotechnological potential for the complete valorization ... ...

    Abstract Biocatalysis is a key tool in both green chemistry and biorefinery fields. NOV1 is a dioxygenase that catalyzes the one-step, coenzyme-free oxidation of isoeugenol into vanillin and holds enormous biotechnological potential for the complete valorization of lignin as a sustainable starting material for biobased chemicals, polymers, and materials. This study integrates computational, kinetic, structural, and biophysical approaches to characterize a new NOV1 variant featuring improved activity and stability compared to those of the wild type. The S283F replacement results in a 2-fold increased turnover rate (
    MeSH term(s) Dioxygenases ; Lignin ; Iron
    Chemical Substances Dioxygenases (EC 1.13.11.-) ; vanillin (CHI530446X) ; Lignin (9005-53-2) ; isoeugenol (5M0MWY797U) ; Iron (E1UOL152H7)
    Language English
    Publishing date 2022-06-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1108-3
    ISSN 1520-4995 ; 0006-2960
    ISSN (online) 1520-4995
    ISSN 0006-2960
    DOI 10.1021/acs.biochem.2c00168
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    Zs.A 217: Show issues Location:
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