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Article ; Online: The mechanism of the Shuang Bai Su Qing recipe in treating periodontitis based on network pharmacology and molecular docking technology.

Zhang, Haomin / Ning, Ruirui / Zhao, Yongqin / Yan, Zhao / Chen, Yongzhi

2023 Volume 102, Issue 44, Page(s) e35139

Abstract: Aim: To explore the material basis of action of the Shuang Bai Su Qing recipe in the treatment ...

Abstract	Aim: To explore the material basis of action of the Shuang Bai Su Qing recipe in the treatment of periodontitis using network pharmacology. Methods: Using TCMSP, we screened the chemical components of 5 drugs. The components were input into the UniProt and PubChem databases to obtain target proteins; Genecards, Online Mendelian Inheritance in Man (OMIM), and CEO databases were used to screen target proteins for periodontal disease. The targets were imported into the Cytoscape software to obtain intersecting targets, and conduct visual analysis to build the PPI network. The intersecting targets were then input into the Matescape database and subjected to biological process (BP) analysis, molecular function (MF) analysis, cell component (CC) analysis, and KEGG enrichment analysis. Results: Twenty-seven TCM chemical components were obtained, with 198 target proteins associated with drugs and 2587 target proteins for periodontitis. Ten core targets were identified. Gene Ontology (GO) functional enrichment analysis yielded results for 20 BP, 11 MF, and 10 CC. KEGG analysis revealed that the main mechanisms of action were related to MAPK signaling pathway. Molecular docking results showed that luteolin strongly bind to TNF, IL6, and IL1B target proteins. Conclusion: The mechanism underlying the treatment of periodontitis with the recipe formula may be closely related to multiple targets in the MAPK signaling pathway.
MeSH term(s)	Humans ; Network Pharmacology ; Molecular Docking Simulation ; Periodontitis ; Periodontal Diseases ; Databases, Genetic ; Drugs, Chinese Herbal
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2023-11-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000035139
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Article ; Online: Active post-transcriptional regulation and ACLY-mediated acetyl-CoA synthesis as a pivotal target of Shuang-Huang-Sheng-Bai formula for lung adenocarcinoma treatment.

Liu, Dan / Dong, Changsheng / Wang, Fengying / Liu, Wei / Jin, Xing / Qi, Sheng-Lan / Liu, Lei / Jin, Qiang / Wang, Siliang / Wu, Jia / Wang, Congcong / Yang, Jing / Deng, Haibin / Cai, Yuejiao / Yang, Lu / Qin, Jingru / Zhang, Chengcheng / Yang, Xi / Wang, Ming-Song /

Yu, Guanzhen / Xue, Yu-Wen / Wang, Zhongqi / Ge, Guang-Bo / Xu, Zhenye / Chen, Wen-Lian

Phytomedicine : international journal of phytotherapy and phytopharmacology

2023 Volume 113, Page(s) 154732

Abstract: ... provide a unique opportunity for improving LC treatment, and the Shuang-Huang-Sheng-Bai (SHSB) formula is ...

Abstract	Background: New therapeutic approaches are required to improve the outcomes of lung cancer (LC), a leading cause of cancer-related deaths worldwide. Chinese herbal medicine formulae widely used in China provide a unique opportunity for improving LC treatment, and the Shuang-Huang-Sheng-Bai (SHSB) formula is a typical example. However, the underlying mechanisms of action remains unclear. Purpose: This study aimed to confirm the efficacy of SHSB against lung adenocarcinoma (LUAD), which is a major histological type of LC, unveil the downstream targets of this formula, and assess the clinical relevance and biological roles of the newly identified target. Methods: An experimental metastasis mouse model and a subcutaneous xenograft mouse model were used to evaluate the anti-cancer activity of SHSB. Multi-omics profiling of subcutaneous tumors and metabolomic profiling of sera were performed to identify downstream targets, especially the metabolic targets of SHSB. A clinical trial was conducted to verify the newly identified metabolic targets in patients. Next, the metabolites and enzymes engaged in the metabolic pathway targeted by SHSB were measured in clinical samples. Finally, routine molecular experiments were performed to decipher the biological functions of the metabolic pathways targeted by SHSB. Results: Oral SHSB administration showed overt anti-LUAD efficacy as revealed by the extended overall survival of the metastasis model and impaired growth of implanted tumors in the subcutaneous xenograft model. Mechanistically, SHSB administration altered protein expression in the post-transcriptional layer and modified the metabolome of LUAD xenografts. Integrative analysis demonstrated that SHSB markedly inhibited acetyl-CoA synthesis in tumors by post-transcriptionally downregulating ATP-citrate lyase (ACLY). Consistently, our clinical trial showed that oral SHSB administration declined serum acetyl-CoA levels of patients with LC. Moreover, acetyl-CoA synthesis and ACLY expression were both augmented in clinical LUAD tissues of patients, and high intratumoral ACLY expression predicted a detrimental prognosis. Finally, we showed that ACLY-mediated acetyl-CoA synthesis is essential for LUAD cell growth by promoting G1/S transition and DNA replication. Conclusion: Limited downstream targets of SHSB for LC treatment have been reported in previous hypothesis-driven studies. In this study, we conducted a comprehensive multi-omics investigation and demonstrated that SHSB exerted its anti-LUAD efficacy by actively and post-transcriptionally modulating protein expression and particularly restraining ACLY-mediated acetyl-CoA synthesis.
MeSH term(s)	Humans ; Mice ; Animals ; ATP Citrate (pro-S)-Lyase/genetics ; ATP Citrate (pro-S)-Lyase/metabolism ; Acetyl Coenzyme A/metabolism ; Drugs, Chinese Herbal/pharmacology ; Adenocarcinoma of Lung/drug therapy ; Lung Neoplasms/drug therapy
Chemical Substances	ATP Citrate (pro-S)-Lyase (EC 2.3.3.8) ; Acetyl Coenzyme A (72-89-9) ; Drugs, Chinese Herbal
Language	English
Publishing date	2023-02-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2023.154732
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Article ; Online: Rapid detection and quality evaluation of Shuang-Huang-Lian injection by ATR-IR and NIR spectroscopy combined with chemometrics.

Ju, Ying / Yin, Chunling / Zhang, Yan / Meng, Xiangru / Zhao, Liuchuang / Hu, Leqian

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2022 Volume 286, Page(s) 122008

Abstract: ... quality evaluation of active components of Shuang-Huang-Lian injection (SHLI), a traditional Chinese medicine ...

Abstract	Quality evaluation and consistency evaluation of drugs are the keys to ensure the therapeutic effect and safety of drugs. In this study, attenuated total refraction infrared (ATR-IR) spectroscopy and near-infrared (NIR) spectroscopy combined with chemometrics were used for rapid detection and quality evaluation of active components of Shuang-Huang-Lian injection (SHLI), a traditional Chinese medicine preparation commonly used in China. Taking the chromatographic detection results as a reference, the partial least squares (PLS) model based on ATR-IR and NIR data was constructed by removing the bands with serious noise interference and low signal frequency band. The results showed that the PLS model achieved satisfactory results for the prediction of the three active components (chlorogenic acid, baicalin and phillyrin) in SHLI, indicating that the two spectral techniques combined with the PLS regression method could be successfully used for rapid quantitative analysis of the three active ingredients in SHLI. Relatively, the PLS model based on the ATR-IR spectrum has higher R
MeSH term(s)	Spectroscopy, Near-Infrared ; Coptis chinensis ; Chemometrics ; Medicine, Chinese Traditional ; Least-Squares Analysis
Language	English
Publishing date	2022-10-20
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2022.122008
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Article ; Online: Mechanism of Shuang-Huang-Lian Oral Liquid for Treatment of Mycoplasmal Pneumonia in Children on Network Pharmacology.

Shi, Ling / Wu, Qi-Guo / Zhang, Ju-Cheng / Yang, Guang-Ming / Liu, Wei / Wang, Ze-Feng

Combinatorial chemistry & high throughput screening

2020 Volume 23, Issue 9, Page(s) 955–971

Abstract: ... system immune damage, and mixed infection in children. The pathogenesis is still unclear. Shuang-Huang-Lian (SHL ...

Abstract	Background and objective: Mycoplasmal pneumonia (MP) can lead to inflammation, multiple system immune damage, and mixed infection in children. The pathogenesis is still unclear. Shuang-Huang-Lian (SHL) oral liquid can treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. However, our current understanding of the molecular mechanisms supporting its clinical application still lags behind due to the lack of researches. It is difficult to understand the overall sensitization mechanism of SHL oral liquid. The purpose is to explain the mechanism of action of drugs in this study, which is useful to ensure the safety of medication for children. Methods: The therapeutic mechanism of SHL oral liquid was investigated by a system pharmacology approach integrating drug-likeness evaluation, oral bioavailability prediction, ADMET, protein-protein interaction worknet, Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes database pathway performance, C-T-P network construction and molecular docking. Results: A total of 18 active ingredients contained in SHL oral liquid and 53 major proteins were screened out as effective players in the treatment of M. pneumoniae disease through some related pathways and molecular docking. The majority of targets, hubs and pathways were highly related to anti-mycoplasma therapy, immunity and inflammation process. Conclusion: This study shows that the anti-bacterial effect of SHL oral liquid has multicomponent, multi-target and multi-pathway phenomena. The proposed approach may provide a feasible tool to clarify the mechanism of traditional Chinese medicines and further develop their therapeutic potentials.
MeSH term(s)	Anti-Infective Agents/chemistry ; Anti-Infective Agents/pharmacokinetics ; Databases, Genetic ; Drug Evaluation, Preclinical ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/pharmacokinetics ; Gene Expression Regulation/drug effects ; Gene Ontology ; Humans ; Medicine, Chinese Traditional ; Molecular Docking Simulation ; Pneumonia, Mycoplasma/drug therapy ; Protein Binding ; Respiratory Tract Infections/drug therapy ; Signal Transduction ; Small Molecule Libraries/chemistry ; Small Molecule Libraries/pharmacology
Chemical Substances	Anti-Infective Agents ; Drugs, Chinese Herbal ; Small Molecule Libraries ; shuang-huang-lian
Language	English
Publishing date	2020-05-14
Publishing country	United Arab Emirates
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2064785-2
ISSN	1875-5402 ; 1386-2073
ISSN (online)	1875-5402
ISSN	1386-2073
DOI	10.2174/1386207323666200514073428
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Zs.A 5577: Show issues

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Article ; Online: Inhibition of Human UDP-Glucuronosyltransferases1A1-Mediated Bilirubin Glucuronidation by the Popular Flavonoids Baicalein, Baicalin, and Hyperoside Is Responsible for Herb (Shuang-Huang-Lian)-Induced Jaundice.

Yang, Xiaolei / Zhu, Guanghao / Zhang, Ying / Wu, Xubo / Liu, Bei / Liu, Ye / Yang, Qing / Du, Wandi / Liang, Jingru / Hu, Jiarong / Yang, Ping / Ge, Guangbo / Cai, Weimin / Ma, Guo

Drug metabolism and disposition: the biological fate of chemicals

2022 Volume 50, Issue 5, Page(s) 552–565

Abstract: ... are not well understood. The aim of this paper was to explore the mechanism of Shuang-huang-lian (SHL ...

Abstract	Bilirubin-related adverse drug reactions (ADRs) or malady (e.g., jaundice) induced by some herbs rich in certain flavonoids have been widely reported. However, the causes and mechanisms of the ADRs are not well understood. The aim of this paper was to explore the mechanism of Shuang-huang-lian (SHL) injections and its major constituents-induced jaundice via inhibiting human UDP-glucuronosyltransferases1A1 (hUGT1A1)-mediated bilirubin glucuronidation. The inhibitory effects of SHL and its major constituents in the herbal medicine, including baicalein (BAI), baicalin (BA), and hyperoside (HYP), on bilirubin glucuroBBREVInidation were investigated. This study indicated that the average formation rates of bilirubin glucuronides [i.e., mono-glucuronide 1 (BMG1), BMG2, and bilirubin diglucuronide] displayed significant differences
MeSH term(s)	Bilirubin ; Coptis chinensis ; Flavanones ; Flavonoids/pharmacology ; Glucuronides ; Glucuronosyltransferase ; Humans ; Jaundice ; Molecular Docking Simulation ; Quercetin/analogs & derivatives ; Uridine Diphosphate
Chemical Substances	Flavanones ; Flavonoids ; Glucuronides ; baicalin (347Q89U4M5) ; baicalein (49QAH60606) ; Uridine Diphosphate (58-98-0) ; hyperoside (8O1CR18L82) ; Quercetin (9IKM0I5T1E) ; Glucuronosyltransferase (EC 2.4.1.17) ; Bilirubin (RFM9X3LJ49)
Language	English
Publishing date	2022-03-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	186795-7
ISSN	1521-009X ; 0090-9556
ISSN (online)	1521-009X
ISSN	0090-9556
DOI	10.1124/dmd.121.000714
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Zs.A 1014: Show issues

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Article ; Online: Comprehensive multicomponent characterization and quality assessment of Shuang-Huang-Lian powder injection using ultra-high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry and ultra-high-performance liquid chromatography-quadrupole-Orbitrap-mass spectrometry.

Yin, Jiaxin / Li, Chao / Zhang, Jie / Ding, Hui / Han, Lifeng / Yang, Wenzhi / Li, Fangyi / Song, Xinbo / Bie, Songtao / Yu, Heshui / Li, Zheng

Rapid communications in mass spectrometry : RCM

2023 Volume 37, Issue 7, Page(s) e9479

Abstract: Rationale: Shuang-Huang-Lian powder injection (SHLPI) is a well-known modern ...

Abstract	Rationale: Shuang-Huang-Lian powder injection (SHLPI) is a well-known modern traditional Chinese medicine formula preparation (TCMFP) widely used to treat acute upper respiratory infections. However, SHLPI is extracted from pure Chinese medicine and administered through an injection, and many adverse reactions have been reported clinically. Therefore, it is necessary to characterize in depth the chemical composition of SHLPI and quantitatively analyze its potential allergenic components. Methods: In this study, the samples were analyzed using ion mobility ultra-high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry (UHPLC-QTOF-MS) combined with a self-built database. Furthermore, the parallel reaction monitoring (PRM) model of ultra-high-performance liquid chromatography-quadrupole-Orbitrap-mass spectrometry (UHPLC-Q-Orbitrap-MS) was used to successfully quantify 10 representative bioactive components. Results: Using this strategy 90 compounds were identified, the fragmentation pathways of five representative compounds in the five main components of SHLPI were summarized, and 10 components (neochlorogenic acid, chlorogenic acid, sweroside, forsythiaside A, luteoloside, isochlorogenic acid B, isochlorogenic acid C, baicalin, phillyrin, and baicalein) were determine as the quality markers of SHLPI based on UPLC-Q-Orbitrap-MS. Conclusions: This work comprehensively characterized the material basis of SHLPI, summarized the cracking laws of representative substances, and quantitatively analyzed 10 potential allergenic components. Therefore, this study could provide a basis for the quality control of SHLPI and the clinical rational use of drugs to reduce its adverse reactions.
MeSH term(s)	Chromatography, High Pressure Liquid/methods ; Powders ; Drugs, Chinese Herbal/chemistry ; Mass Spectrometry
Chemical Substances	shuang-huang-lian ; Powders ; Drugs, Chinese Herbal
Language	English
Publishing date	2023-01-23
Publishing country	England
Document type	Journal Article
ZDB-ID	58731-x
ISSN	1097-0231 ; 0951-4198
ISSN (online)	1097-0231
ISSN	0951-4198
DOI	10.1002/rcm.9479
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Article ; Online: An on-line analytical approach for detecting haptens in Shuang-huang-lian powder injection.

Wang, Hong / Zhang, Xiaotian / Wang, Daidong / Sun, Hongyang / Lan, Yuexiang / Jiang, Haixiu / Chen, Shizhong / Lin, Zongtao

Journal of chromatography. A

2017 Volume 1513, Page(s) 126–139

Abstract: Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI ...

Abstract	Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI) frequently used in the clinical treatment of faucitis, bronchitis, and other viral and bacterial infections of upper respiratory tract. However, its allergenic reactions, being the main adverse effects (AEs) of SHLPI, have been a serious problem of its clinical safety. This problem has not been solved due to short of methods for detecting haptens in complex TCMIs. In this study, an on-line high-performance liquid chromatography-diode-array detector-mass spectrometry combined with bovine serum albumin-fluorescence detector (HPLC-DAD-MS-BSA-FLD) system was established for the first time, validated and applied for identification of haptens in SHLPI. Fourteen of 35 identified compounds showed BSA binding activity, and they were six flavonoids, six caffeoylquinic acids (CQAs), and two phenylethanoid glycosides. The structure-activity relationships of 10 active components were studied, and their ability of sensitization together with that of two CQAs were further verified by ELISA assay. It was found that 10 compounds had sensitization, and flavonoids showed stronger sensitizability than CQAs while the diCQAs were slightly stronger than caffeoylquinic acids. The system was validated using 3-CQA as a positive control, and was proved to have good reproducibility, stability, precision (RSD<0.1%) and linearity (R
Language	English
Publishing date	2017-09-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2017.07.031
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Article ; Online: Discovery of anti-flu substances and mechanism of Shuang-Huang-Lian water extract based on serum pharmaco-chemistry and network pharmacology.

Zhang, Feng-Xiang / Li, Zi-Ting / Yang, Xia / Xie, Zhi-Neng / Chen, Ming-Hao / Yao, Zhi-Hong / Chen, Jian-Xin / Yao, Xin-Sheng / Dai, Yi

Journal of ethnopharmacology

2020 Volume 268, Page(s) 113660

Abstract: Ethnopharmacological relevance: Shuang-Huang-Lian preparation has captured wide attention ... aimed to reveal the anti-flu substances and mechanism of Shuang-Huang-Lian water extract (SHL) on H1N1 ...

Abstract	Ethnopharmacological relevance: Shuang-Huang-Lian preparation has captured wide attention since its clinical applications for the successful treatment of upper respiratory tract infection. However, its functional basis under actual therapeutic dose in vivo was still unrevealed. Aim of the study: This study aimed to reveal the anti-flu substances and mechanism of Shuang-Huang-Lian water extract (SHL) on H1N1 infected mouse model by a strategy based on serum pharmaco-chemistry under actual therapeutic dose and network pharmacology. Materials and methods: H1N1 infected mouse model was employed for evaluation of the anti-flu effects of SHL. A simultaneous quantification method was developed by UPLC-TQ-XS MS coupled switch-ions mode and applied to characterize the pharmacokinetics of the multiple components of SHL under actual therapeutic dose. The potential active ingredients were screened out based on their pharmacokinetic parameters. And then, a compound mixture of these active candidates was re-evaluated for the anti-flu activity on H1N1 infected mouse model. Furthermore, the anti-flu mechanism of SHL was also predicted by network pharmacology coupled with the experimental result. Results: SHL significantly increased the survival rate and prolonged survival days on H1N1 infected mice at a dosage of 20 g crude drug/kg/day by reversing the increased lung index, down-regulating the inflammatory cytokines (TNF-α, IL-1β, IL-6) and inhibiting the release of IFN-β in bronchoalveolar lavage fluids (BALF). Concomitantly, the pharmacokinetic parameters of fourteen quantified and twenty-one semi-quantified constituents of SHL were characterized. And then, five compounds (baicalin, sweroside, chlorogenic acid, forsythoside A and phillyrin), which displayed satisfactory pharmacokinetic features, were considered as potential active ingredients. Thus, a mixture of these five ingredients was administered to H1N1-infected mice at a dose of 4.24 mg/kg/day. As a result, the therapeutical effects of the mixture were similar to SHL in terms of survival rate, lung index and the release of cytokines (TNF-α, IL-1β and IL-6) in BALF. Moreover, network pharmacology analysis indicated that the TNF-signal pathways might play a role in the anti-flu mechanism of SHL. Conclusions: A mixture of five compounds (baicalin, sweroside, chlorogenic acid, forsythoside A and phillyrin) were the anti-flu substances of SHL. The strategy based on serum pharmaco-chemistry under actual therapeutic dose provided a new sight on exploring in vivo effective substances of TCM.
MeSH term(s)	Animals ; Drug Discovery/methods ; Drugs, Chinese Herbal/isolation & purification ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Influenza A Virus, H1N1 Subtype/drug effects ; Influenza A Virus, H1N1 Subtype/physiology ; Male ; Mice ; Mice, Inbred BALB C ; Orthomyxoviridae Infections/drug therapy ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Protein Interaction Maps/drug effects ; Protein Interaction Maps/physiology ; Water/pharmacology
Chemical Substances	Drugs, Chinese Herbal ; Plant Extracts ; shuang-huang-lian ; Water (059QF0KO0R)
Language	English
Publishing date	2020-12-01
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.113660
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Article: Discovery of anti-flu substances and mechanism of Shuang-Huang-Lian water extract based on serum pharmaco-chemistry and network pharmacology

Zhang, Feng-xiang / Li, Zi-ting / Yang, Xia / Xie, Zhi-neng / Chen, Ming-hao / Yao, Zhi-hong / Chen, Jian-xin / Yao, Xin-sheng / Dai, Yi

Journal of ethnopharmacology. 2021 Mar. 25, v. 268

2021

Abstract: Shuang-Huang-Lian preparation has captured wide attention since its clinical applications ... of Shuang-Huang-Lian water extract (SHL) on H1N1 infected mouse model by a strategy based on serum pharmaco ...

Abstract	Shuang-Huang-Lian preparation has captured wide attention since its clinical applications for the successful treatment of upper respiratory tract infection. However, its functional basis under actual therapeutic dose in vivo was still unrevealed.This study aimed to reveal the anti-flu substances and mechanism of Shuang-Huang-Lian water extract (SHL) on H1N1 infected mouse model by a strategy based on serum pharmaco-chemistry under actual therapeutic dose and network pharmacology.H1N1 infected mouse model was employed for evaluation of the anti-flu effects of SHL. A simultaneous quantification method was developed by UPLC-TQ-XS MS coupled switch-ions mode and applied to characterize the pharmacokinetics of the multiple components of SHL under actual therapeutic dose. The potential active ingredients were screened out based on their pharmacokinetic parameters. And then, a compound mixture of these active candidates was re-evaluated for the anti-flu activity on H1N1 infected mouse model. Furthermore, the anti-flu mechanism of SHL was also predicted by network pharmacology coupled with the experimental result.SHL significantly increased the survival rate and prolonged survival days on H1N1 infected mice at a dosage of 20 g crude drug/kg/day by reversing the increased lung index, down-regulating the inflammatory cytokines (TNF-α, IL-1β, IL-6) and inhibiting the release of IFN-β in bronchoalveolar lavage fluids (BALF). Concomitantly, the pharmacokinetic parameters of fourteen quantified and twenty-one semi-quantified constituents of SHL were characterized. And then, five compounds (baicalin, sweroside, chlorogenic acid, forsythoside A and phillyrin), which displayed satisfactory pharmacokinetic features, were considered as potential active ingredients. Thus, a mixture of these five ingredients was administered to H1N1-infected mice at a dose of 4.24 mg/kg/day. As a result, the therapeutical effects of the mixture were similar to SHL in terms of survival rate, lung index and the release of cytokines (TNF-α, IL-1β and IL-6) in BALF. Moreover, network pharmacology analysis indicated that the TNF-signal pathways might play a role in the anti-flu mechanism of SHL.A mixture of five compounds (baicalin, sweroside, chlorogenic acid, forsythoside A and phillyrin) were the anti-flu substances of SHL. The strategy based on serum pharmaco-chemistry under actual therapeutic dose provided a new sight on exploring in vivo effective substances of TCM.
Keywords	baicalin ; blood serum ; chlorogenic acid ; drugs ; interleukin-6 ; lungs ; mice ; pharmacokinetics ; respiratory tract diseases ; survival rate ; therapeutics ; traditional medicine
Language	English
Dates of publication	2021-0325
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.113660
Database	NAL-Catalogue (AGRICOLA)

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Article: An on-line analytical approach for detecting haptens in Shuang-huang-lian powder injection

Wang, Hong / Xiaotian Zhang / Daidong Wang / Hongyang Sun / Yuexiang Lan / Haixiu Jiang / Shizhong Chen / Zongtao Lin

Journal of chromatography. 2017,

2017

Abstract: Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI ...

Abstract	Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI) frequently used in the clinical treatment of faucitis, bronchitis, and other viral and bacterial infections of upper respiratory tract. However, its allergenic reactions, being the main adverse effects (AEs) of SHLPI, have been a serious problem of its clinical safety. This problem has not been solved due to short of methods for detecting haptens in complex TCMIs. In this study, an on-line high-performance liquid chromatography-diode-array detector-mass spectrometry combined with bovine serum albumin-fluorescence detector (HPLC-DAD-MS-BSA-FLD) system was established for the first time, validated and applied for identification of haptens in SHLPI. Fourteen of 35 identified compounds showed BSA binding activity, and they were six flavonoids, six caffeoylquinic acids (CQAs), and two phenylethanoid glycosides. The structure-activity relationships of 10 active components were studied, and their ability of sensitization together with that of two CQAs were further verified by ELISA assay. It was found that 10 compounds had sensitization, and flavonoids showed stronger sensitizability than CQAs while the diCQAs were slightly stronger than caffeoylquinic acids. The system was validated using 3-CQA as a positive control, and was proved to have good reproducibility, stability, precision (RSD<0.1%) and linearity (R2>0.9993). This online system is fast, sensitive and efficient for screening haptens in traditional Chinese medicine injection (TCMI), provides a new approach to reveal the chemical basis of haptens in TCMIs.
Keywords	Oriental traditional medicine ; adverse effects ; allergenicity ; bacterial infections ; blood serum ; bronchitis ; cattle ; chlorogenic acid ; chromatography ; enzyme-linked immunosorbent assay ; flavonoids ; glycosides ; haptens ; respiratory system ; screening ; spectroscopy ; structure-activity relationships
Language	English
Size	p. .
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2017.07.031
Database	NAL-Catalogue (AGRICOLA)

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