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Article ; Online: A hyperthermia-enhanced nanocatalyst based on asymmetric Au@polypyrrole for synergistic cancer Fenton/photothermal therapy.

Wu, Xixi / Liang, Huazhen / Li, Chaoming / Zhou, Duanyang / Liu, Rui

RSC advances

2023 Volume 13, Issue 41, Page(s) 29061–29069

Abstract: The specific tumor microenvironment is a conducive breeding ground for malignant tumors, favoring their survival, rapid proliferation, and metastasis, which is also an inevitable obstacle to tumor treatment, particularly for catalytic therapy. To address ...

Abstract	The specific tumor microenvironment is a conducive breeding ground for malignant tumors, favoring their survival, rapid proliferation, and metastasis, which is also an inevitable obstacle to tumor treatment, particularly for catalytic therapy. To address this issue, a hyperthermia-enhanced nanocatalyst (AuP@MnO
Language	English
Publishing date	2023-10-04
Publishing country	England
Document type	Journal Article
ISSN	2046-2069
ISSN (online)	2046-2069
DOI	10.1039/d3ra04779b
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Article ; Online: [Multicellular coupling fermentation for 3'-sialyllactose conversion using N-acetyl-glucosamine and lactose].

Zhou, Wen / You, Xing / Zhang, Hongtao / Li, Zhongxia / Deng, Chaoming / Xu, Chun / Li, Yu

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

2023 Volume 39, Issue 11, Page(s) 4621–4634

Abstract: Sialyllactose is one of the most abundant sialylated oligosaccharides in human milk oligosaccharides (HMOs), which plays an important role in the healthy development of infants and young children. However, its efficient and cheap production technology is ...

Abstract	Sialyllactose is one of the most abundant sialylated oligosaccharides in human milk oligosaccharides (HMOs), which plays an important role in the healthy development of infants and young children. However, its efficient and cheap production technology is still lacking presently. This study developed a two-step process employing multiple-strains for the production of sialyllactose. In the first step, two engineered strains,
MeSH term(s)	Child ; Humans ; Child, Preschool ; N-Acetylneuraminic Acid ; Escherichia coli/genetics ; Lactose ; Fermentation ; Saccharomyces cerevisiae ; Oligosaccharides ; Glucosamine
Chemical Substances	N-acetylneuraminoyllactose (3J7TAL60G3) ; 3'-sialyllactose ; N-Acetylneuraminic Acid (GZP2782OP0) ; Lactose (J2B2A4N98G) ; Oligosaccharides ; Glucosamine (N08U5BOQ1K)
Language	Chinese
Publishing date	2023-09-15
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1042206-7
ISSN	1872-2075 ; 1042-749X
ISSN (online)	1872-2075
ISSN	1042-749X
DOI	10.13345/j.cjb.230152
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Zs.A 3505: Show issues

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Article: Research progress of LINE-1 in the diagnosis, prognosis, and treatment of gynecologic tumors.

Fu, Jiaojiao / Qin, Tiansheng / Li, Chaoming / Zhu, Jiaojiao / Ding, Yaoyao / Zhou, Meiying / Yang, Qing / Liu, Xiaofeng / Zhou, Juanhong / Chen, Fan

Frontiers in oncology

2023 Volume 13, Page(s) 1201568

Abstract: The retrotransposon known as long interspersed nuclear element-1 (LINE-1), which is currently the sole autonomously mobile transposon in the human genome, can result in insertional mutations, chromosomal rearrangements, and genomic instability. In recent ...

Abstract	The retrotransposon known as long interspersed nuclear element-1 (LINE-1), which is currently the sole autonomously mobile transposon in the human genome, can result in insertional mutations, chromosomal rearrangements, and genomic instability. In recent years, numerous studies have shown that LINE-1 is involved in the development of various diseases and also plays an important role in the immune regulation of the organism. The expression of LINE-1 in gynecologic tumors suggests that it is expected to be an independent indicator for early diagnosis and prognosis, and also, as a therapeutic target, LINE-1 is closely associated with gynecologic tumor prognosis. This article discusses the function of LINE-1 in the diagnosis, treatment, and prognosis of ovarian, cervical, and endometrial malignancies, as well as other gynecologic malignancies. It offers fresh perspectives on the early detection of tumors and the creation of novel anti-tumor medications.
Language	English
Publishing date	2023-07-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1201568
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Article ; Online: Electroactive Hydrogels with Photothermal/Photodynamic Effects for Effective Wound Healing Assisted by Polydopamine-Modified Graphene Oxide.

Xie, Chaoming / Luo, Jiaqing / Luo, Yongjie / Zhou, Jie / Guo, Xiaochuan / Lu, Xiong

ACS applied materials & interfaces

2023 Volume 15, Issue 36, Page(s) 42329–42340

Abstract: Antibacterial hydrogel wound dressings have attracted considerable attention in recent years. However, bacterial infections can occur at any point during the wound-healing process. There is a demand for hydrogels that possess on-demand antibacterial and ... ...

Abstract	Antibacterial hydrogel wound dressings have attracted considerable attention in recent years. However, bacterial infections can occur at any point during the wound-healing process. There is a demand for hydrogels that possess on-demand antibacterial and excellent wound repair properties. Herein, we report a near-infrared (NIR)-light-responsive indocyanine green (ICG)-loaded polydopamine (PDA)-mediated graphene oxide (PGO) and amorphous calcium phosphate (CaP)-incorporated poly(vinyl alcohol) (PVA) hydrogel using a mussel-inspired approach. PGO was reduced by PDA, which endowed the hydrogel with electroactivity and provided abundant sites for loading ICG. Amorphous CaP was formed in situ in the PVA hydrogel to enhance its mechanical properties and biocompatibility. Taking advantage of the high photothermal and photodynamic efficiency of ICG-PGO, the ICG-PGO-CaP-PVA hydrogel exhibited fascinating on-demand antibacterial activity through NIR light irradiation. Moreover, the thermally induced gel-sol conversion of PVA accelerated the release of Ca ions and allowed the hydrogel to adapt to irregular wounds. Meanwhile, PGO endows the hydrogel with conductivity and cell affinity, which facilitate endogenous electrical signal transfer to control cell behavior. In vitro and in vivo studies demonstrated that the ICG-PGO-CaP-PVA hydrogel exhibited a strong tissue repair activity under NIR light irradiation. This mussel-inspired strategy offers a novel way to design hydrogel dressings for wound healing.
MeSH term(s)	Hydrogels/pharmacology ; Indoles/pharmacology ; Indocyanine Green ; Anti-Bacterial Agents/pharmacology
Chemical Substances	polydopamine ; Hydrogels ; graphene oxide ; Indoles ; Indocyanine Green (IX6J1063HV) ; amorphous calcium phosphate ; Anti-Bacterial Agents
Language	English
Publishing date	2023-08-30
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c09860
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Article ; Online: Double J stent combined with pyelostomy tube in pediatric laparoscopic pyeloplasty: a 5-year clinical experience in a single center.

Chen, Zhiqiang / Wang, Yunjin / Wu, Changwei / Chen, Hong / Cui, Xu / Zhou, Chaoming

BMC urology

2023 Volume 23, Issue 1, Page(s) 181

Abstract: Objective: To compare the outcome of using a double J (DJ) stent combined with pyelostomy tube with a DJ stent alone in laparoscopic pyeloplasty (LP) for pediatric ureteropelvic junction obstruction (UPJO).: Methods: A retrospective review of all ... ...

Abstract	Objective: To compare the outcome of using a double J (DJ) stent combined with pyelostomy tube with a DJ stent alone in laparoscopic pyeloplasty (LP) for pediatric ureteropelvic junction obstruction (UPJO). Methods: A retrospective review of all patients with UPJO treated with LP between January 2017 and November 2021 was conducted in our center. According to different postoperative drainage methods patients were divided into a DJ stent group (52 cases) and a DJ stent combined with pyelostomy tube group (combination group, 41 cases). Operative time, bleeding volume, perirenal drainage stent removal time, postoperative hospital stay, postoperative complications, and renal function recovery were compared between the two groups. Renal ultrasound and diuretic renogram (DR) were used for preoperative and postoperative follow-up. Results: A total of 52 patients were in the DJ stent group and 41 patients in the combination group. The mean hospital stay was 6.46 ± 2.66 days in the DJ stent group and 5.22 ± 1.63 days in the combination group (p < 0.05). Postoperative complications developed in 14 out of 52 patients in the DJ stent group (26.9%), while complications developed in 8 out of 41 patients in the combination group (19.5%) (p > 0.05). Non-catheter-related complications developed in 10/52 patients in the DJ stent group (19.2%) and only 1/41 patients in the combination group (2.4%) (p < 0.05). The renal function and renal cortex thickness in both groups were improved. Conclusion: Both the DJ stent drainage and the DJ stent combined with pyelostomy drainage are safe and effective. We should fully consider the patient's preoperative and intraoperative conditions and choose appropriate drainage methods. A DJ stent combined with pyelostomy tube can reduce non-catheter related complications, facilitate postoperative recovery, and the hospital stay was significantly shorter than the DJ stent group. However, it is necessary to pay attention to the nursing treatment of the pyelostomy tube and guard against the occurrence of pyelostomy tube shedding.
MeSH term(s)	Child ; Humans ; Kidney Pelvis/surgery ; Laparoscopy/methods ; Urologic Surgical Procedures/methods ; Kidney/physiology ; Ureteral Obstruction/surgery ; Nephrotomy ; Retrospective Studies ; Stents ; Postoperative Complications/epidemiology ; Postoperative Complications/surgery ; Treatment Outcome
Language	English
Publishing date	2023-11-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2059857-9
ISSN	1471-2490 ; 1471-2490
ISSN (online)	1471-2490
ISSN	1471-2490
DOI	10.1186/s12894-023-01351-1
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Article ; Online: Pembrolizumab combined with anlotinib improves therapeutic efficacy in pulmonary sarcomatoid carcinoma with TMB-H and PD-L1 expression: a case report and literature review.

Wu, Shugui / Wu, Shanlian / Liao, Xiaohong / Zhou, Chaoming / Qiu, Feng / Wang, Chen / Zhong, Wenjuan

Frontiers in immunology

2023 Volume 14, Page(s) 1274937

Abstract: Background: Pulmonary sarcomatoid carcinoma (PSC) is a unique subtype of non-small cell lung cancer (NSCLC) with a high degree of malignancy and poor therapeutic effects. With the widespread use of immune checkpoint inhibitors (ICIs) in recent years, ... ...

Abstract	Background: Pulmonary sarcomatoid carcinoma (PSC) is a unique subtype of non-small cell lung cancer (NSCLC) with a high degree of malignancy and poor therapeutic effects. With the widespread use of immune checkpoint inhibitors (ICIs) in recent years, few studies have reported that immunotherapy is effective against PSC. As a multi-target anti-vascular targeting agent, anlotinib showed a better anti-tumor effect in various cancer species. The paper reported the therapeutic and side effects of pembrolizumab combined with anlotinib in a patient with advanced PSC. Case presentation: This is a 73 year old female patient who underwent thoracoscopy right upper lobectomy and was diagnosed as locally advanced PSC. However, the patient experienced tumor recurrence and metastasis 7 weeks after surgery and was unable to tolerate chemoradiotherapy. Moreover, she detected TP53 mutation and found that tumor mutation burden (TMB) and PD-L1 were high expression. Therefore, the patient received pembrolizumab combined with anlotinib treatment. After 15 cycles of treatment, the tumor significantly shrank with no tumor activity. The evaluation of tumor efficacy is partial response (PR). During the treatment period, she experienced one-degree thyroid-stimulating hormone elevation and two-degree hand-foot syndrome. Pembrolizumab and anlotinib was continued for two years as a maintenance treatment. The patient had a good quality of life and no disease progression was observed. Currently, the patient is still alive without tumor progression and has overall survival exceeding 45 months and toxic side effects were tolerable. Conclusions: Combining ICIs and anti-angiogenic targeted therapy has brought new hope in treating advanced PSC. Additionally, TMB and PD-L1 expression could be potential predictive biomarkers of the efficacy in advanced PSC with immunotherapy.
MeSH term(s)	Female ; Humans ; Aged ; Carcinoma, Non-Small-Cell Lung/pathology ; Lung Neoplasms/pathology ; B7-H1 Antigen ; Quality of Life ; Antineoplastic Agents, Immunological/adverse effects ; Neoplasm Recurrence, Local/drug therapy ; Biomarkers, Tumor/genetics ; Carcinoma/drug therapy
Chemical Substances	pembrolizumab (DPT0O3T46P) ; anlotinib ; B7-H1 Antigen ; Antineoplastic Agents, Immunological ; Biomarkers, Tumor
Language	English
Publishing date	2023-10-23
Publishing country	Switzerland
Document type	Review ; Case Reports ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1274937
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Article ; Online: Evaluating testicular function changes in unilateral cryptorchid chinese infants underwent orchidopexy in the first year of life.

Huang, Wenhua / Xu, Longyao / Chen, Shushen / Chen, Zhiqiang / Cui, Xu / Zhou, Chaoming

International urology and nephrology

2023 Volume 56, Issue 5, Page(s) 1537–1542

Abstract: Objective: Management of cryptorchidism is typically recommended within the first 18 months of life to maximize fertility potential. However, there is a paucity of longitudinal postoperative data for Chinese infants. We aim to evaluate the Testicular ... ...

Abstract	Objective: Management of cryptorchidism is typically recommended within the first 18 months of life to maximize fertility potential. However, there is a paucity of longitudinal postoperative data for Chinese infants. We aim to evaluate the Testicular function change when the procedure is done within the first year of life. Method: We prospectively enrolled 51 children diagnosed with unilateral inguinal cryptorchidism into the surgical group between January 2021 and January 2022. Orchidopexy was carried out through a single transverse scrotal incision. Assessments of anti-Mullerian hormone (AMH), inhibin B (InhB), testosterone (T) levels, testicular volume and testicular atrophy index (TAI) were conducted at baseline, 6 months, and 1 year following surgery. Concurrently, clinical data from 42 healthy age-matched controls were collected during their routine physical examinations. Results: At 6- and 12-months post-surgery, testicular volume increased significantly to 0.98 ± 0.12 mL and 1.01 ± 0.12ml. AMH levels also rose from 76.40 ± 15.77 ng/mL to 81.52 ± 15.32 ng/mL and 87.50 ± 15.36 ng/mL. However, these parameters are significantly lower than age-matched healthy controls (both P < 0.001). InhB levels significantly increased after surgery and even surpassed those of healthy controls after 6 months (both P < 0.001). The TAI was 16.7% and 8.6% at 6- and 12-months following surgery. Conclusion: Although orchiopexy can improve testicular growth and function, the restoration of testicular function to the level of healthy peers might take longer. To expedite the recovery of testicular function and bring it in line with that of peers, we recommend addressing cryptorchidism at the earliest opportunity.
MeSH term(s)	Male ; Child ; Infant ; Humans ; Cryptorchidism/surgery ; Orchiopexy ; Testis/surgery ; Anti-Mullerian Hormone ; China
Chemical Substances	Anti-Mullerian Hormone (80497-65-0)
Language	English
Publishing date	2023-12-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	204048-7
ISSN	1573-2584 ; 0301-1623 ; 0042-1162
ISSN (online)	1573-2584
ISSN	0301-1623 ; 0042-1162
DOI	10.1007/s11255-023-03896-4
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Zs.A 733: Show issues

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Article ; Online: Goliath induces inflammation in obese mice by linking fatty acid β-oxidation to glycolysis.

Hao, Shumeng / Zhang, Sulin / Ye, Jialin / Chen, Lifan / Wang, Yan / Pei, Siyu / Zhu, Qingchen / Xu, Jing / Tao, Yongzhen / Zhou, Neng / Yin, Huiyong / Duan, Cai-Wen / Mao, Chaoming / Zheng, Mingyue / Xiao, Yichuan

EMBO reports

2023 Volume 24, Issue 4, Page(s) e56932

Abstract: Obesity is associated with metabolic disorders and chronic inflammation. However, the obesity-associated metabolic contribution to inflammatory induction remains elusive. Here, we show that, compared with lean mice, ... ...

Abstract	Obesity is associated with metabolic disorders and chronic inflammation. However, the obesity-associated metabolic contribution to inflammatory induction remains elusive. Here, we show that, compared with lean mice, CD4
MeSH term(s)	Animals ; Mice ; Mice, Obese ; Fatty Acids/metabolism ; Inflammation/metabolism ; Obesity/metabolism ; Glycolysis ; Ubiquitin-Protein Ligases/metabolism ; Oxidation-Reduction
Chemical Substances	Fatty Acids ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
Language	English
Publishing date	2023-03-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020896-0
ISSN	1469-3178 ; 1469-221X
ISSN (online)	1469-3178
ISSN	1469-221X
DOI	10.15252/embr.202356932
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Zs.A 5433: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 41: Show issues

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Article ; Online: Interpenetrating nanofibrillar membrane of self-assembled collagen and antimicrobial peptides for enhanced bone regeneration.

Dai, Jinhong / Fischer, Nicholas G / Rahimi, Joseph R / Wang, Hongning / Hu, Chaoming / Chen, Wener / Lin, Yifan / Sang, Ting / Chew, Hooi Pin / Kong, Liang / Aparicio, Conrado / Ye, Zhou / Huang, Shengbin

International journal of biological macromolecules

2024 Volume 267, Issue Pt 1, Page(s) 131480

Abstract: Bone regeneration remains a major clinical challenge, especially when infection necessitates prolonged antibiotic treatment. This study presents a membrane composed of self-assembled and interpenetrating GL13K, an antimicrobial peptide (AMP) derived from ...

Abstract	Bone regeneration remains a major clinical challenge, especially when infection necessitates prolonged antibiotic treatment. This study presents a membrane composed of self-assembled and interpenetrating GL13K, an antimicrobial peptide (AMP) derived from a salivary protein, in a collagen membrane for antimicrobial activity and enhanced bone regeneration. Commercially available collagen membranes were immersed in GL13K solution, and self-assembly was initiated by raising the solution pH to synthesize the multifunctional membrane called COL-GL. COL-GL was composed of interpenetrating large collagen fibers and short GL13K nanofibrils, which increased hydrophobicity, reduced biodegradation from collagenase, and stiffened the matrix compared to control collagen membranes. Incorporation of GL13K led to antimicrobial and anti-fouling activity against early oral surface colonizer Streptococcus gordonii while not affecting fibroblast cytocompatibility or pre-osteoblast osteogenic differentiation. GL13K in solution also reduced macrophage inflammatory cytokine expression and increased pro-healing cytokine expression. Bone formation in a rat calvarial model was accelerated at eight weeks with COL-GL compared to the gold-standard collagen membrane based on microcomputed tomography and histology. Interpenetration of GL13K within collagen sidesteps challenges with antimicrobial coatings on bone regeneration scaffolds while increasing bone regeneration. This strength makes COL-GL a promising approach to reduce post-surgical infections and aid bone regeneration in dental and orthopedic applications. STATEMENT OF SIGNIFICANCE: The COL-GL membrane, incorporating the antimicrobial peptide GL13K within a collagen membrane, signifies a noteworthy breakthrough in bone regeneration strategies for dental and orthopedic applications. By integrating self-assembled GL13K nanofibers into the membrane, this study successfully addresses the challenges associated with antimicrobial coatings, exhibiting improved antimicrobial and anti-fouling activity while preserving compatibility with fibroblasts and pre-osteoblasts. The accelerated bone formation observed in a rat calvarial model emphasizes the potential of this innovative approach to minimize post-surgical infections and enhance bone regeneration outcomes. As a promising alternative for future therapeutic interventions, this material tackles the clinical challenges of extended antibiotic treatments and antibiotic resistance in bone regeneration scenarios.
Language	English
Publishing date	2024-04-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.131480
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Zs.B 2374: Show issues

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Article ; Online: Identification and Characterization of RK22, a Novel Antimicrobial Peptide from

Lu, Xiaoyu / Yang, Min / Zhou, Shengwen / Yang, Shuo / Chen, Xiran / Khalid, Mehwish / Wang, Kexin / Fang, Yaqun / Wang, Chaoming / Lai, Ren / Duan, Zilei

International journal of molecular sciences

2023 Volume 24, Issue 17

Abstract: Staphylococcus ... ...

Abstract	Staphylococcus aureus
MeSH term(s)	Animals ; Methicillin-Resistant Staphylococcus aureus ; Staphylococcus aureus ; Antimicrobial Peptides ; Leeches ; Staphylococcal Infections/drug therapy
Chemical Substances	Antimicrobial Peptides
Language	English
Publishing date	2023-08-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241713453
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