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Article ; Online: Granulopoiesis requires increased C/EBPα compared to monopoiesis, correlated with elevated Cebpa in immature G-CSF receptor versus M-CSF receptor expressing cells.

Ma, Ou / Hong, SunHwa / Guo, Hong / Ghiaur, Gabriel / Friedman, Alan D

PloS one

2014 Volume 9, Issue 4, Page(s) e95784

Abstract: C/EBPα is required for the formation of granulocyte-monocyte progenitors; however, its role ... in accumulating blasts. Indicating that Cebpa is the relevant shRNA target, shRNA-resistant C/EBPα-ER rescued ... Sca-1-c-Kit+ cells into GCSFR+MCSFR- or GCSFR-MCSFR+ subsets. Cebpa, Cebpe, Gfi1, Ets1, and Klf5 RNAs ...

Abstract	C/EBPα is required for the formation of granulocyte-monocyte progenitors; however, its role in subsequent myeloid lineage specification remains uncertain. Transduction of murine marrow with either of two Cebpa shRNAs markedly increases monocyte and reduces granulocyte colonies in methylcellulose or the monocyte to neutrophil ratio in liquid culture. Similar findings were found after marrow shRNA transduction and transplantation and with CEBPA knockdown in human marrow CD34+ cells. These results apparently reflect altered myeloid lineage specification, as similar knockdown allowed nearly complete 32Dcl3 granulocytic maturation. Cebpa knockdown also generated lineage-negative blasts with increased colony replating capacity but unchanged cell cycle parameters, likely reflecting complete differentiation block. The shRNA having the greatest effect on lineage skewing reduced Cebpa 3-fold in differentiating cells but 6-fold in accumulating blasts. Indicating that Cebpa is the relevant shRNA target, shRNA-resistant C/EBPα-ER rescued marrow myelopoiesis. Cebpa knockdown in murine marrow cells also increased in vitro erythropoiesis, perhaps reflecting 1.6-fold reduction in PU.1 leading to GATA-1 derepression. Global gene expression analysis of lineage-negative blasts that accumulate after Cebpa knockdown demonstrated reduction in Cebpe and Gfi1, known transcriptional regulators of granulopoiesis, and also reduced Ets1 and Klf5. Populations enriched for immature granulocyte or monocyte progenitor/precursors were isolated by sorting Lin-Sca-1-c-Kit+ cells into GCSFR+MCSFR- or GCSFR-MCSFR+ subsets. Cebpa, Cebpe, Gfi1, Ets1, and Klf5 RNAs were increased in the c-Kit+GCSFR+ and Klf4 and Irf8 in the c-Kit+MCSFR+ populations, with PU.1 levels similar in both. In summary, higher levels of C/EBPα are required for granulocyte and lower levels for monocyte lineage specification, and this myeloid bifurcation may be facilitated by increased Cebpa gene expression in granulocyte compared with monocyte progenitors.
MeSH term(s)	Animals ; Blotting, Western ; CCAAT-Enhancer-Binding Protein-alpha/genetics ; CCAAT-Enhancer-Binding Protein-alpha/metabolism ; CCAAT-Enhancer-Binding Proteins/genetics ; CCAAT-Enhancer-Binding Proteins/metabolism ; Cells, Cultured ; Flow Cytometry ; Granulocytes/cytology ; Granulocytes/metabolism ; Hematopoiesis/genetics ; Hematopoiesis/physiology ; Mice ; Mice, Inbred C57BL ; Myelopoiesis/genetics ; Myelopoiesis/physiology ; Receptor, Macrophage Colony-Stimulating Factor/genetics ; Receptor, Macrophage Colony-Stimulating Factor/metabolism ; Receptors, Granulocyte Colony-Stimulating Factor/genetics ; Receptors, Granulocyte Colony-Stimulating Factor/metabolism ; Thrombopoietin/genetics ; Thrombopoietin/metabolism
Chemical Substances	CCAAT-Enhancer-Binding Protein-alpha ; CCAAT-Enhancer-Binding Proteins ; CEBPA protein, mouse ; Receptors, Granulocyte Colony-Stimulating Factor ; Thrombopoietin (9014-42-0) ; Receptor, Macrophage Colony-Stimulating Factor (EC 2.7.10.1)
Language	English
Publishing date	2014-04-21
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0095784
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Granulopoiesis requires increased C/EBPα compared to monopoiesis, correlated with elevated Cebpa in immature G-CSF receptor versus M-CSF receptor expressing cells.

Ou Ma / SunHwa Hong / Hong Guo / Gabriel Ghiaur / Alan D Friedman

PLoS ONE, Vol 9, Iss 4, p e

2014 Volume 95784

Abstract	C/EBPα is required for the formation of granulocyte-monocyte progenitors; however, its role in subsequent myeloid lineage specification remains uncertain. Transduction of murine marrow with either of two Cebpa shRNAs markedly increases monocyte and reduces granulocyte colonies in methylcellulose or the monocyte to neutrophil ratio in liquid culture. Similar findings were found after marrow shRNA transduction and transplantation and with CEBPA knockdown in human marrow CD34+ cells. These results apparently reflect altered myeloid lineage specification, as similar knockdown allowed nearly complete 32Dcl3 granulocytic maturation. Cebpa knockdown also generated lineage-negative blasts with increased colony replating capacity but unchanged cell cycle parameters, likely reflecting complete differentiation block. The shRNA having the greatest effect on lineage skewing reduced Cebpa 3-fold in differentiating cells but 6-fold in accumulating blasts. Indicating that Cebpa is the relevant shRNA target, shRNA-resistant C/EBPα-ER rescued marrow myelopoiesis. Cebpa knockdown in murine marrow cells also increased in vitro erythropoiesis, perhaps reflecting 1.6-fold reduction in PU.1 leading to GATA-1 derepression. Global gene expression analysis of lineage-negative blasts that accumulate after Cebpa knockdown demonstrated reduction in Cebpe and Gfi1, known transcriptional regulators of granulopoiesis, and also reduced Ets1 and Klf5. Populations enriched for immature granulocyte or monocyte progenitor/precursors were isolated by sorting Lin-Sca-1-c-Kit+ cells into GCSFR+MCSFR- or GCSFR-MCSFR+ subsets. Cebpa, Cebpe, Gfi1, Ets1, and Klf5 RNAs were increased in the c-Kit+GCSFR+ and Klf4 and Irf8 in the c-Kit+MCSFR+ populations, with PU.1 levels similar in both. In summary, higher levels of C/EBPα are required for granulocyte and lower levels for monocyte lineage specification, and this myeloid bifurcation may be facilitated by increased Cebpa gene expression in granulocyte compared with monocyte progenitors.
Keywords	Medicine ; R ; Science ; Q
Subject code	616
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article: Involvement of mu- and m-calpains and protein kinase C isoforms in L8 myoblast differentiation.

Liang, Yu-Chuan / Yeh, Jan-Ying / Forsberg, Neil E / Ou, Bor-Rung

The international journal of biochemistry & cell biology

2006 Volume 38, Issue 4, Page(s) 662–670

Abstract: The objectives were to investigate the roles of different calpains and protein kinase C (PKC ... isoforms in muscle differentiation. Concentrations of mu- and m-calpain increased significantly ... whereas PKCalpha and delta declined significantly during L8 myoblast differentiation. Both mu-calpain and m-calpain ...

Abstract	The objectives were to investigate the roles of different calpains and protein kinase C (PKC) isoforms in muscle differentiation. Concentrations of mu- and m-calpain increased significantly whereas PKCalpha and delta declined significantly during L8 myoblast differentiation. Both mu-calpain and m-calpain antisense oligonucleotides inhibited myotube formation and creatine kinase activity during L8 myoblast differentiation. These results implied that both mu- and m-calpain were involved in L8 myoblast differentiation. To investigate the involvement of calpain in regulation of PKC concentrations, mu-calpain antisense oligonucleotides were added to L8 myoblasts. PKCalpha remained unchanged and PKCdelta declined. By adding m-calpain antisense oligonucleotides instead, PKCalpha level remained unchanged and PKCdelta concentrations increased significantly during differentiation. These results suggest that PKCalpha, but not PKCdelta, is the substrate for mu-calpain and PKCalpha and delta are the substrates for the m-calpain. In addition, more phosphorylated myogenin was found in day 2 antisense oligonucleotides treated L8 cells. It is concluded that the decline of PKCalpha mediated by m- and mu-calpain is essential for L8 myoblast differentiation. The decline of PKC during myoblast differentiation may cause hypo-phosphorylation of myogenin, which in turn activates muscle-specific genes during myogenesis.
MeSH term(s)	Animals ; Calpain/antagonists & inhibitors ; Calpain/genetics ; Calpain/metabolism ; Cell Differentiation/drug effects ; Cell Differentiation/physiology ; Cell Line ; Enzyme Activation/drug effects ; Enzyme Activation/genetics ; Gene Expression Regulation, Enzymologic/drug effects ; Gene Expression Regulation, Enzymologic/physiology ; Myoblasts/metabolism ; Oligodeoxyribonucleotides, Antisense/genetics ; Oligodeoxyribonucleotides, Antisense/pharmacology ; Protein Kinase C-alpha/genetics ; Protein Kinase C-alpha/metabolism ; Protein Kinase C-delta/genetics ; Protein Kinase C-delta/metabolism ; Rats
Chemical Substances	Oligodeoxyribonucleotides, Antisense ; Protein Kinase C-alpha (EC 2.7.11.13) ; Protein Kinase C-delta (EC 2.7.11.13) ; Calpain (EC 3.4.22.-) ; m-calpain (EC 3.4.22.-) ; mu-calpain (EC 3.4.22.-)
Language	English
Publishing date	2006
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1228429-4
ISSN	1878-5875 ; 1357-2725
ISSN (online)	1878-5875
ISSN	1357-2725
DOI	10.1016/j.biocel.2005.11.009
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Article ; Online: Selpercatinib as the Guardian of the Central Nervous System for Patients With RET Fusion-Positive NSCLC?

Lau, Sally C M / Ou, Sai-Hong Ignatius

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

2023 Volume 18, Issue 5, Page(s) 561–563

MeSH term(s)	Humans ; Central Nervous System/drug effects ; Lung Neoplasms/drug therapy ; Lung Neoplasms/genetics ; Proto-Oncogene Proteins c-ret/genetics ; Pyrazoles ; Pyridines
Chemical Substances	Proto-Oncogene Proteins c-ret (EC 2.7.10.1) ; Pyrazoles ; Pyridines ; RET protein, human (EC 2.7.10.1) ; selpercatinib (CEGM9YBNGD)
Language	English
Publishing date	2023-04-21
Publishing country	United States
Document type	Editorial ; Comment
ZDB-ID	2432037-7
ISSN	1556-1380 ; 1556-0864
ISSN (online)	1556-1380
ISSN	1556-0864
DOI	10.1016/j.jtho.2023.02.005
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Article ; Online: Glyoxal in Foods: Formation, Metabolism, Health Hazards, and Its Control Strategies.

Zhang, Mianzhang / Huang, Caihuan / Ou, Juanying / Liu, Fu / Ou, Shiyi / Zheng, Jie

Journal of agricultural and food chemistry

2024 Volume 72, Issue 5, Page(s) 2434–2450

Abstract: Glyoxal is a highly reactive aldehyde widely present in common diet and environment and inevitably generated through various metabolic ... ...

Abstract	Glyoxal is a highly reactive aldehyde widely present in common diet and environment and inevitably generated through various metabolic pathways
MeSH term(s)	Humans ; Glyoxal/chemistry ; Lipid Peroxidation ; Maillard Reaction ; Food
Chemical Substances	Glyoxal (50NP6JJ975)
Language	English
Publishing date	2024-01-29
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c08225
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Article: The Physical Resilience Instrument for Older Adults (PRIFOR) in Surgical Inpatients: Further Evidence for Its Factor Structure and Validity.

Lin, C-Y / Ou, C-H / Chang, C-M / Hu, F-W

The Journal of frailty & aging

2023 Volume 12, Issue 2, Page(s) 91–96

Abstract: Background: The Physical Resilience Instrument for Older Adults (PRIFOR) is a questionnaire for assessing physical resilience in older adults suffering from acute health stressors. Prior psychometric evidence of the PRIFOR showed that it has good ... ...

Abstract	Background: The Physical Resilience Instrument for Older Adults (PRIFOR) is a questionnaire for assessing physical resilience in older adults suffering from acute health stressors. Prior psychometric evidence of the PRIFOR showed that it has good criterion-related validity, known-group validity, predictive validity, and internal consistency. However, it is unclear whether the PRIFOR can be replicated in older adults suffering after surgical treatment. Objectives: This study aimed at evaluating whether the three-factor structure of the PRIFOR can be replicated in older adults suffering after surgical treatment. Moreover, the concurrent validity of the PRIFOR was examined using the association between the PRIFOR and measures of depression, cognition, activities of daily living, and frailty. Design and setting: A longitudinal study was adopted in a tertiary-care medical center in Taiwan. Participants: A total of 207 patients aged 65 years old and older who underwent surgery and if they were able to communicate independently. Measurements: The PRIFOR, the 5-item Geriatric Depression Scale, the Short Portable Mental Status Questionnaire, the Katz Index of Independence in Activities of Daily Living and Clinical Frailty Scale were all assessed after surgery. Results: The three-factor structure (positive thinking, cope and adjust lifestyle, and belief and hopeful mindset) was supported by the CFA results in the present sample. In addition, the PRIFOR showed good concurrent validity with depression (r = -0.470 to -0.542), cognition (r = 0.358 to 0.409), activities of daily living (r = 0.209 to 0.310), and frailty (r =-0.161 to -0.237). Conclusion: The PRIFOR can be recommended to measure physical resilience in older adults suffering after surgical treatment. For the adequate estimation of older adults' level of physical resilience postoperatively and to guide the implementation of individualized interventions, it is important to provide appropriate care for older adults to recover after surgery.
MeSH term(s)	Aged ; Humans ; Frailty/diagnosis ; Activities of Daily Living ; Inpatients ; Longitudinal Studies ; Geriatric Assessment/methods ; Psychometrics ; Surveys and Questionnaires ; Reproducibility of Results
Language	English
Publishing date	2023-03-22
Publishing country	France
Document type	Journal Article
ZDB-ID	2856228-8
ISSN	2273-4309 ; 2260-1341
ISSN (online)	2273-4309
ISSN	2260-1341
DOI	10.14283/jfa.2023.8
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Article ; Online: Approaching complete genomes, transcriptomes and epi-omes with accurate long-read sequencing.

Kovaka, Sam / Ou, Shujun / Jenike, Katharine M / Schatz, Michael C

Nature methods

2023 Volume 20, Issue 1, Page(s) 12–16

MeSH term(s)	Transcriptome ; Sequence Analysis, DNA ; High-Throughput Nucleotide Sequencing
Language	English
Publishing date	2023-01-01
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2169522-2
ISSN	1548-7105 ; 1548-7091
ISSN (online)	1548-7105
ISSN	1548-7091
DOI	10.1038/s41592-022-01716-8
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Article: Exosome derived from tumor-associated macrophages: biogenesis, functions, and therapeutic implications in human cancers.

Zhou, Manli / He, Xiaoyun / Mei, Cheng / Ou, Chunlin

Biomarker research

2023 Volume 11, Issue 1, Page(s) 100

Abstract: Tumor-associated macrophages (TAMs), one of the most abundant immune cell types in the tumor microenvironment (TME), account for approximately 50% of the local hematopoietic cells. TAMs play an important role in tumorigenesis and tumor development ... ...

Abstract	Tumor-associated macrophages (TAMs), one of the most abundant immune cell types in the tumor microenvironment (TME), account for approximately 50% of the local hematopoietic cells. TAMs play an important role in tumorigenesis and tumor development through crosstalk between various immune cells and cytokines in the TME. Exosomes are small extracellular vesicles with a diameter of 50-150 nm, that can transfer biological information (e.g., proteins, nucleic acids, and lipids) from secretory cells to recipient cells through the circulatory system, thereby influencing the progression of various human diseases, including cancer. Recent studies have suggested that TAMs-derived exosomes play crucial roles in malignant cell proliferation, invasion, metastasis, angiogenesis, immune responses, drug resistance, and tumor metabolic reprogramming. TAMs-derived exosomes have the potential to be targeted for tumor therapy. In addition, the abnormal expression of non-coding RNAs and proteins in TAMs-derived exosomes is closely related to the clinicopathological features of patients with cancer, and these exosomes are expected to become new liquid biopsy markers for the early diagnosis, prognosis, and monitoring of tumors. In this review, we explored the role of TAMs-derived exosomes in tumorigenesis to provide new diagnostic biomarkers and therapeutic targets for cancer prevention.
Language	English
Publishing date	2023-11-19
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2699926-2
ISSN	2050-7771
ISSN	2050-7771
DOI	10.1186/s40364-023-00538-w
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Article ; Online: Formation of Hesperetin-Methylglyoxal Adducts in Food and

Zhang, Mianzhang / Ge, Tiansi / Huang, Weijian / He, Jun / Huang, Caihuan / Ou, Juanying / Ou, Shiyi / Zheng, Jie

Journal of agricultural and food chemistry

2024

Abstract: Polyphenols with a ... ...

Abstract	Polyphenols with a typical
Language	English
Publishing date	2024-04-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.4c00481
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Article ; Online: And Still They Come Over Troubled Waters: Can Asia's Third-Generation EGFR Tyrosine Kinase Inhibitors (Furmonertinib, Aumolertinib, Rezivertinib, Limertinib, Befotertinib, SH-1028, and Lazertinib) Affect Global Treatment of EGFR+ NSCLC.

Lau, Sally C M / Ou, Sai-Hong Ignatius

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

2022 Volume 17, Issue 10, Page(s) 1144–1154

MeSH term(s)	Acrylamides ; Asia ; Carcinoma, Non-Small-Cell Lung/drug therapy ; ErbB Receptors/genetics ; Humans ; Indoles ; Lung Neoplasms/drug therapy ; Morpholines ; Mutation ; Protein Kinase Inhibitors/pharmacology ; Protein Kinase Inhibitors/therapeutic use ; Pyrazoles ; Pyrimidines
Chemical Substances	Acrylamides ; Indoles ; Morpholines ; Protein Kinase Inhibitors ; Pyrazoles ; Pyrimidines ; lazertinib (4A2Y23XK11) ; EGFR protein, human (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1) ; aumolertinib (T4RS462G19)
Language	English
Publishing date	2022-10-03
Publishing country	United States
Document type	Editorial
ZDB-ID	2432037-7
ISSN	1556-1380 ; 1556-0864
ISSN (online)	1556-1380
ISSN	1556-0864
DOI	10.1016/j.jtho.2022.08.016
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