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Article ; Online: Role of peptidoglycan recycling enzymes AmpD and AnmK in

Tajuelo, Ana / Terrón, María C / López-Siles, Mireia / McConnell, Michael J

Frontiers in cellular and infection microbiology

2023 Volume 12, Page(s) 1064053

Abstract: Acinetobacter ... ...

Abstract	Acinetobacter baumannii
MeSH term(s)	Fosfomycin/pharmacology ; Virulence ; Acinetobacter baumannii/metabolism ; Peptidoglycan/metabolism ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism ; Biofilms ; Drug Resistance, Multiple, Bacterial
Chemical Substances	Fosfomycin (2N81MY12TE) ; Peptidoglycan ; Anti-Bacterial Agents
Language	English
Publishing date	2023-01-13
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2022.1064053
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Article ; Online: Perception of care from the perspective of nursing students: A study using photovoice, storytelling and poetry.

Andina-Díaz, Elena / Siles-González, José / Gutiérrez-García, Ana Isabel / Solano-Ruiz, MCarmen

Nurse education in practice

2023 Volume 72, Page(s) 103791

Abstract: Background: The Higher Education in Nursing requires providing students with skills such as critical and reflective thinking about the actions to be carried out so that they are capable of providing humanised and quality care in an increasingly complex ... ...

Abstract	Background: The Higher Education in Nursing requires providing students with skills such as critical and reflective thinking about the actions to be carried out so that they are capable of providing humanised and quality care in an increasingly complex and technified society. Participatory teaching methodologies promote the development of these skills. Objectives: to explore the potential of photovoice, storytelling and poetry as instruments capable of encouraging reflection. To identify through images (photovoice) and poetic narratives (storytelling and poetry) the perception that students of the nursing degree express about nursing care. Design: qualitative study in the framework of the socio-critical paradigm, using photovoice, storytelling and poetry in the field of care. Participants: first-year undergraduate nursing students at two Spanish universities in the academic year 2021-2022. Methodology: 48 images were shown and students were asked to select one, write a narrative and generate a short poem connected to the image. Subsequently, a content analysis was conducted of those poems evoked by the five photographs most chosen by the students. The analysis was supported by qualitative data analysis software. A total of 67 contributions from 67 students were analysed. Results: the concepts addressed in the poems and the compositions generated reveal how critical humanist thinking and reflection on the subject of care developed. The topics identified were care, accompaniment throughout the life cycle, working on the basis of values and caring for small details as subcategories of nursing care. Conclusion: The combination of different tools in university education has encouraged reflection and enabled students to identify for themselves the importance of humanised care. Working on the theoretical foundation in an active way can reduce the gap between theory and practice and foster the empowerment of the nursing identity.
MeSH term(s)	Humans ; Education, Nursing, Baccalaureate/methods ; Students, Nursing ; Thinking ; Nursing Education Research ; Perception
Language	English
Publishing date	2023-09-26
Publishing country	Scotland
Document type	Journal Article
ZDB-ID	2058575-5
ISSN	1873-5223 ; 1471-5953
ISSN (online)	1873-5223
ISSN	1471-5953
DOI	10.1016/j.nepr.2023.103791
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Article: Novel bioactive extract from yarrow obtained by the supercritical antisolvent-assisted technique inhibits lipid metabolism in colorectal cancer.

Gómez de Cedrón, Marta / Siles-Sanchez, Maria de Las Nieves / Martín-Hernandez, Diego / Jaime, Laura / Santoyo, Susana / Ramírez de Molina, Ana

Frontiers in bioengineering and biotechnology

2024 Volume 12, Page(s) 1256190

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2024-03-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2024.1256190
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Article ; Online: Photovoice: An active learning tool with community nursing students.

Andina-Díaz, Elena / Welch, Lindsay / Siles-González, José / Serrano-Fuentes, Nestor / Gutiérrez-García, Ana Isabel / Solano-Ruiz, MCarmen

Public health nursing (Boston, Mass.)

2024 Volume 41, Issue 3, Page(s) 392–402

Abstract: Objective: To assess nursing students' experiences of using photovoice as a pedagogical approach to active learning in the community.: Methods: A descriptive design with a cross-sectional mixed-method questionnaire was used with 108 students ... ...

Abstract	Objective: To assess nursing students' experiences of using photovoice as a pedagogical approach to active learning in the community. Methods: A descriptive design with a cross-sectional mixed-method questionnaire was used with 108 students following an educational activity, in which their communities were photographed and the impact of the pandemic on vulnerable populations was reflected. Descriptive statistics and thematic analysis were used to analyze the data. Results: Seventy eight percent of the students felt that photovoice was an interesting and useful tool for nurses, 89% affirmed it helped stimulate reflection on social and health inequities in times of pandemic, 82% described that it developed many emotions and feelings and 86% would like to disseminate their photographs directly to stakeholders, citizens, and politicians. Three themes were identified in the data: "stimulate critical reflection", "develop emotional skills", and "encourage action". Conclusions: Photovoice is a successful active learning pedagogical approach that engages nursing students to develop critical awareness while connecting with their communities, with the real world. It fosters students' sensitivity and motivation and encourages them to take action. Teachers need to introduce new scaffolds for active learning, such as photovoice, to provide innovative academic support that nurtures and develops the next generation of nurses appropriately.
MeSH term(s)	Humans ; Problem-Based Learning/methods ; Education, Nursing, Baccalaureate/methods ; Students, Nursing/psychology ; Cross-Sectional Studies ; Curriculum
Language	English
Publishing date	2024-02-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	632563-4
ISSN	1525-1446 ; 0737-1209
ISSN (online)	1525-1446
ISSN	0737-1209
DOI	10.1111/phn.13285
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Article ; Online: Corrigendum: Brief Research Report: Virus-Specific Humoral Immunity at Admission Predicts the Development of Respiratory Failure in Unvaccinated SARS-CoV-2 Patients.

Tajuelo, Ana / Carretero, Octavio / García-Ríos, Estéfani / López-Siles, Mireia / Cano, Olga / Vázquez, Mónica / Más, Vicente / Rodríguez-Goncer, Isabel / Lalueza, Antonio / López-Medrano, Francisco / San Juan, Rafael / Fernández-Ruiz, Mario / Aguado, José M / McConnell, Michael J / Pérez-Romero, Pilar

Frontiers in immunology

2022 Volume 13, Page(s) 965809

Abstract: This corrects the article DOI: 10.3389/fimmu.2022.878812.]. ...

Abstract	[This corrects the article DOI: 10.3389/fimmu.2022.878812.].
Language	English
Publishing date	2022-06-30
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.965809
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Article ; Online: Photovoice and dramatisation in the classroom with nursing students: An exploratory study to raise awareness of the cultural and social dimensions of violence against women.

Solano-Ruiz, MCarmen / Andina-Díaz, Elena / Noreña-Peña, Ana / Siles-González, José

Nurse education today

2021 Volume 103, Page(s) 104974

Abstract: Background: Active teaching methodologies that incorporate students' feelings and beliefs and encourage them to reflect upon and critically think about certain variables, are extremely useful to teachers.: Objective: To explore the potential of ... ...

Abstract	Background: Active teaching methodologies that incorporate students' feelings and beliefs and encourage them to reflect upon and critically think about certain variables, are extremely useful to teachers. Objective: To explore the potential of dramatisation and photovoice technologies as instruments to stimulate nursing students to reflect upon violence against women in society, thereby encouraging critical thinking and debate in a participatory way. Design: This was a qualitative, descriptive-exploratory study which used the participatory action research approach. Participants: Forty fourth-year students enrolled in the Nursing undergraduate degree at the University of Alicante in Spain. Methods: The photovoice technique was used to record images from a dramatisation which served as the basis to represent scenes from the everyday life of people who are subjected to physical and psychological violence. These photographs were then debated and analysed by the students. We used MAXQDA® software and the guidelines published by Giorgi to aid our data analysis. Results: Four main categories emerged; the 'violence against women'. The 'victim' and 'abuser' categories considered 'emotions', 'feelings', 'attitudes', and 'behaviours'. The fourth category that emerged was 'social viewpoints'. Conclusions: Photovoice-dramatisation raised awareness among the students and allowed them to reflect upon these behaviours and to critically think about the cultural and social dimensions of violence against women. The use of both of these techniques in practical and theoretical contexts to train students can help to nurture social commitment when faced with this problem.
MeSH term(s)	Female ; Humans ; Spain ; Students, Nursing ; Thinking ; Violence
Language	English
Publishing date	2021-05-18
Publishing country	Scotland
Document type	Journal Article
ZDB-ID	1062570-7
ISSN	1532-2793 ; 0260-6917
ISSN (online)	1532-2793
ISSN	0260-6917
DOI	10.1016/j.nedt.2021.104974
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Article: Subinhibitory Concentrations of Clinically-Relevant Antimicrobials Affect Resistance-Nodulation-Division Family Promoter Activity in

Prieto Martin Gil, Sonia / Tajuelo, Ana / López-Siles, Mireia / McConnell, Michael J

Frontiers in microbiology

2021 Volume 12, Page(s) 780201

Abstract: Efflux pumps contribute to multidrug resistance ... ...

Abstract	Efflux pumps contribute to multidrug resistance in
Language	English
Publishing date	2021-12-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2021.780201
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Article ; Online: Integral approach using bacterial microbiome to stabilize municipal solid waste.

Jurado, Macarena M / Camelo-Castillo, Anny J / Suárez-Estrella, Francisca / López, Maria J / López-González, Juan A / Estrella-González, Maria J / Síles-Castellano, Ana B / Moreno, Joaquín

Journal of environmental management

2020 Volume 265, Page(s) 110528

Abstract: Biological transformation of municipal solid waste is an environment-friendly management strategy against recalcitrant residues. The bacterial biome that inhabit said residues are responsible of decomposing both simple and complex materials. For this ... ...

Abstract	Biological transformation of municipal solid waste is an environment-friendly management strategy against recalcitrant residues. The bacterial biome that inhabit said residues are responsible of decomposing both simple and complex materials. For this reason, processes such as composting, which favor the acceleration of the transformation of organic matter, can contribute to the degradation of municipal solid waste. Not only as mere fertilizer for crops, but also as methods for the recovery of solid waste. However, the control of the conditions necessary to achieve an optimal process on an industrial scale is a great concern. Thus, the aim of this work focuses on the characterization of the bacterial microbiome on three municipal solid waste facilities in order to deepen the role of microorganisms in the state of the final product obtained. For it, an intensive metagenomic analysis as well as a battery of physicochemical determinations were carried out. The lack of adequate thermophilic phases was decisive in finding certain bacterial genera, such as Lactobacillus, which was significant through these processes. Biodiversity did not follow a common pattern in the three processes, neither in abundance nor in richness but, in general, it was greater during the bio-oxidative stage. Despite the different trend in terms of the degradation of carbon fractions in these wastes, at the end of the biodegradation treatments, a sufficient degree of bioestabilization of the organic matter was reached. The results offer the opportunity to obtain a level of detail unprecedented of the structure, dynamics and function of the bacterial community in real conditions, without the control offered by laboratory conditions or pilot plants.
MeSH term(s)	Composting ; Fertilizers ; Microbiota ; Refuse Disposal ; Soil ; Solid Waste ; Waste Management
Chemical Substances	Fertilizers ; Soil ; Solid Waste
Language	English
Publishing date	2020-04-13
Publishing country	England
Document type	Journal Article
ZDB-ID	184882-3
ISSN	1095-8630 ; 0301-4797
ISSN (online)	1095-8630
ISSN	0301-4797
DOI	10.1016/j.jenvman.2020.110528
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Article ; Online: Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells.

Calahorra, Jesús / Martínez-Lara, Esther / Granadino-Roldán, José M / Martí, Juan M / Cañuelo, Ana / Blanco, Santos / Oliver, F Javier / Siles, Eva

Scientific reports

2020 Volume 10, Issue 1, Page(s) 6361

Abstract: Olive oil intake has been linked with a lower incidence of breast cancer. Hypoxic microenvironment in solid tumors, such as breast cancer, is known to play a crucial role in cancer progression and in the failure of anticancer treatments. HIF-1 is the ... ...

Abstract	Olive oil intake has been linked with a lower incidence of breast cancer. Hypoxic microenvironment in solid tumors, such as breast cancer, is known to play a crucial role in cancer progression and in the failure of anticancer treatments. HIF-1 is the foremost effector in hypoxic response, and given that hydroxytyrosol (HT) is one of the main bioactive compounds in olive oil, in this study we deepen into its modulatory role on HIF-1. Our results in MCF-7 breast cancer cells demonstrate that HT decreases HIF-1α protein, probably by downregulating oxidative stress and by inhibiting the PI3K/Akt/mTOR pathway. Strikingly, the expression of HIF-1 target genes does not show a parallel decrease. Particularly, adrenomedullin and vascular endothelial growth factor are up-regulated by high concentrations of HT even in HIF-1α silenced cells, pointing to HIF-1-independent mechanisms of regulation. In fact, we show, by in silico modelling and transcriptional analysis, that high doses of HT may act as an agonist of the aryl hydrocarbon receptor favoring the induction of these angiogenic genes. In conclusion, we suggest that the effect of HT in a hypoxic environment is largely affected by its concentration and involves both HIF-1 dependent and independent mechanisms.
MeSH term(s)	Breast Neoplasms/drug therapy ; Breast Neoplasms/genetics ; Female ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/genetics ; MCF-7 Cells ; Olive Oil/chemistry ; Olive Oil/pharmacology ; Phenol/chemistry ; Phenol/pharmacology ; Phenylethyl Alcohol/analogs & derivatives ; Phenylethyl Alcohol/metabolism ; Phenylethyl Alcohol/pharmacology ; Phosphatidylinositol 3-Kinases/genetics ; Proto-Oncogene Proteins c-akt/genetics ; Signal Transduction/drug effects ; TOR Serine-Threonine Kinases/genetics
Chemical Substances	HIF1A protein, human ; Hypoxia-Inducible Factor 1, alpha Subunit ; Olive Oil ; 3,4-dihydroxyphenylethanol (10597-60-1) ; Phenol (339NCG44TV) ; MTOR protein, human (EC 2.7.1.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; TOR Serine-Threonine Kinases (EC 2.7.11.1) ; Phenylethyl Alcohol (ML9LGA7468)
Language	English
Publishing date	2020-04-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-63417-6
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Article ; Online: Multiple-bead assay for the differential serodiagnosis of neglected human cestodiases: Neurocysticercosis and cystic echinococcosis.

Hernández-González, Ana / González-Bertolín, Belén / Urrea, Laura / Fleury, Agnes / Ferrer, Elizabeth / Siles-Lucas, Mar / Tamarozzi, Francesca / Perteguer, Maria J

PLoS neglected tropical diseases

2022 Volume 16, Issue 1, Page(s) e0010109

Abstract: Background: Neurocysticercosis (NCC), and cystic echinococcosis (CE) are two neglected diseases caused by cestodes, co-endemic in many areas of the world. Imaging studies and serological tests are used in the diagnosis of both parasitic diseases, but ... ...

Abstract	Background: Neurocysticercosis (NCC), and cystic echinococcosis (CE) are two neglected diseases caused by cestodes, co-endemic in many areas of the world. Imaging studies and serological tests are used in the diagnosis of both parasitic diseases, but cross-reactions may confound the results of the latter. The novel multiplex bead-based assay with recombinant antigens has been reported to increases the diagnostic accuracy of serological techniques. Methodology: We set-up an immunoassay based on the multiplex bead-based platform (MBA), using the rT24H (against Cysticercus cellulosae, causing cysticercosis) and r2B2t (against Echinococcus granulosus sensu lato, causing CE) recombinant antigens, for simultaneous and differential diagnosis of these infections. The antigens were tested on 356 sera from 151 patients with CE, 126 patients with NCC, and 79 individuals negative for both diseases. Specificity was calculated including sera from healthy donors, other neurological diseases and the respective NCC or CE sera counterpart. The diagnostic accuracy of this assay was compared with two commercial ELISA tests, Novalisa and Ridascreen, widely used in the routine diagnosis of cysticercosis and CE, respectively. Main findings: For the diagnosis of NCC, sensitivity ranged from 57.94-63.49% for the rT24H-MBA, and 40.48-46.03% for Novalisa ELISA depending on exclusion or inclusion of sera having equivocal results on ELISA from the analysis; specificities ranged from 90.87-91.30% and 70.43-76.96%, respectively. AUC values of the ROC curve were 0.783 (rT24H) and 0.619 (Novalisa) (p-value < 0.001). For the diagnosis of CE, the sensitivity of the r2B2t-MBA ranged from 68.87-69.77% and of Ridascreen ELISA from 50.00-57.62%; specificities from 92.47-92.68% and from 74.15-80.98%, respectively. AUC values were 0.717 and 0.760, respectively. Conclusions/significance: Overall, the recombinant antigens tested with the bead-based technology showed better diagnostic accuracy than the commercial assays, particularly for the diagnosis of NCC. The possibility of testing the same serum sample simultaneously for the presence of antibodies against both antigens is an added value particularly in seroprevalence studies for cysticercosis linked to control programs in endemic areas where these two parasites coexist.
MeSH term(s)	Animals ; Antibodies, Helminth/blood ; Antigens, Helminth/genetics ; Antigens, Helminth/immunology ; Echinococcosis/diagnosis ; Echinococcosis/parasitology ; Echinococcus granulosus/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Neglected Diseases/diagnosis ; Neglected Diseases/parasitology ; Neurocysticercosis/diagnosis ; Neurocysticercosis/parasitology ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Sensitivity and Specificity ; Serologic Tests ; Taenia solium/immunology
Chemical Substances	Antibodies, Helminth ; Antigens, Helminth ; Recombinant Proteins
Language	English
Publishing date	2022-01-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2429704-5
ISSN	1935-2735 ; 1935-2735
ISSN (online)	1935-2735
ISSN	1935-2735
DOI	10.1371/journal.pntd.0010109
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