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  1. Article ; Online: Commentary on Letter to the Editor After "Repair of Finger Pulp Defect and Sensory Reconstruction Using Reverse Homodigital Artery Island Flap With Palmar Cutaneous Branches of the Proper Digital Nerve" by Shuang Wang, Annals of Plastic Surgery, 2023.

    Wang, Shuang / Yuan, Chaoqun / Ding, Wenqiang / Shen, Haobo / Gu, Jiaxiang

    Annals of plastic surgery

    2023  Volume 91, Issue 6, Page(s) 789–790

    MeSH term(s) Humans ; Surgery, Plastic ; Surgical Flaps/blood supply ; Plastic Surgery Procedures ; Fingers/blood supply ; Arteries/surgery
    Language English
    Publishing date 2023-09-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 423835-7
    ISSN 1536-3708 ; 0148-7043
    ISSN (online) 1536-3708
    ISSN 0148-7043
    DOI 10.1097/SAP.0000000000003687
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    Zs.A 1419: Show issues Location:
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  2. Article: Exploring the molecular mechanism of Gan Shuang granules for the treatment of non-alcoholic steatohepatitis using network pharmacology, molecular docking, and experimental verification.

    Zhi, Guoguo / Shao, Bingjie / Zheng, Tianyan / Mu, Jie / Li, Jingwei / Feng, Yiyuan / Zhu, Sha / Dang, Yanni / Liu, Feng / Wang, Dong

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1082451

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2023-01-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1082451
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  3. Article ; Online: Active post-transcriptional regulation and ACLY-mediated acetyl-CoA synthesis as a pivotal target of Shuang-Huang-Sheng-Bai formula for lung adenocarcinoma treatment.

    Liu, Dan / Dong, Changsheng / Wang, Fengying / Liu, Wei / Jin, Xing / Qi, Sheng-Lan / Liu, Lei / Jin, Qiang / Wang, Siliang / Wu, Jia / Wang, Congcong / Yang, Jing / Deng, Haibin / Cai, Yuejiao / Yang, Lu / Qin, Jingru / Zhang, Chengcheng / Yang, Xi / Wang, Ming-Song /
    Yu, Guanzhen / Xue, Yu-Wen / Wang, Zhongqi / Ge, Guang-Bo / Xu, Zhenye / Chen, Wen-Lian

    Phytomedicine : international journal of phytotherapy and phytopharmacology

    2023  Volume 113, Page(s) 154732

    Abstract: ... provide a unique opportunity for improving LC treatment, and the Shuang-Huang-Sheng-Bai (SHSB) formula is ...

    Abstract Background: New therapeutic approaches are required to improve the outcomes of lung cancer (LC), a leading cause of cancer-related deaths worldwide. Chinese herbal medicine formulae widely used in China provide a unique opportunity for improving LC treatment, and the Shuang-Huang-Sheng-Bai (SHSB) formula is a typical example. However, the underlying mechanisms of action remains unclear.
    Purpose: This study aimed to confirm the efficacy of SHSB against lung adenocarcinoma (LUAD), which is a major histological type of LC, unveil the downstream targets of this formula, and assess the clinical relevance and biological roles of the newly identified target.
    Methods: An experimental metastasis mouse model and a subcutaneous xenograft mouse model were used to evaluate the anti-cancer activity of SHSB. Multi-omics profiling of subcutaneous tumors and metabolomic profiling of sera were performed to identify downstream targets, especially the metabolic targets of SHSB. A clinical trial was conducted to verify the newly identified metabolic targets in patients. Next, the metabolites and enzymes engaged in the metabolic pathway targeted by SHSB were measured in clinical samples. Finally, routine molecular experiments were performed to decipher the biological functions of the metabolic pathways targeted by SHSB.
    Results: Oral SHSB administration showed overt anti-LUAD efficacy as revealed by the extended overall survival of the metastasis model and impaired growth of implanted tumors in the subcutaneous xenograft model. Mechanistically, SHSB administration altered protein expression in the post-transcriptional layer and modified the metabolome of LUAD xenografts. Integrative analysis demonstrated that SHSB markedly inhibited acetyl-CoA synthesis in tumors by post-transcriptionally downregulating ATP-citrate lyase (ACLY). Consistently, our clinical trial showed that oral SHSB administration declined serum acetyl-CoA levels of patients with LC. Moreover, acetyl-CoA synthesis and ACLY expression were both augmented in clinical LUAD tissues of patients, and high intratumoral ACLY expression predicted a detrimental prognosis. Finally, we showed that ACLY-mediated acetyl-CoA synthesis is essential for LUAD cell growth by promoting G1/S transition and DNA replication.
    Conclusion: Limited downstream targets of SHSB for LC treatment have been reported in previous hypothesis-driven studies. In this study, we conducted a comprehensive multi-omics investigation and demonstrated that SHSB exerted its anti-LUAD efficacy by actively and post-transcriptionally modulating protein expression and particularly restraining ACLY-mediated acetyl-CoA synthesis.
    MeSH term(s) Humans ; Mice ; Animals ; ATP Citrate (pro-S)-Lyase/genetics ; ATP Citrate (pro-S)-Lyase/metabolism ; Acetyl Coenzyme A/metabolism ; Drugs, Chinese Herbal/pharmacology ; Adenocarcinoma of Lung/drug therapy ; Lung Neoplasms/drug therapy
    Chemical Substances ATP Citrate (pro-S)-Lyase (EC 2.3.3.8) ; Acetyl Coenzyme A (72-89-9) ; Drugs, Chinese Herbal
    Language English
    Publishing date 2023-02-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2023.154732
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4115: Show issues Location:
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  4. Article ; Online: Development of a UPLC-MS/MS method for the determination of active ingredients of Shuang Hu in rat blood and its application in pharmacokinetics.

    Jiang, Xin Rui / Lei, Meng Ying / Qin, Liu Xin / Zhou, Yan Lin / Wang, Yang / Li, Xue Jian / Wang, Gang

    Biomedical chromatography : BMC

    2022  , Page(s) e5533

    Abstract: ... to evaluate the pharmacokinetics of Shuang Hu tincture (SHZTN). Xevo TQ-S was used to analyze the active ...

    Abstract A simple and sensitive method using in vivo microdialysis coupled with UPLC-MS/MS was established to evaluate the pharmacokinetics of Shuang Hu tincture (SHZTN). Xevo TQ-S was used to analyze the active ingredients of mesaconitine, hypaconitine, 4-hydroxycinnamic acid, ferulic acid and N-(2, 3-dimethyl phenyl)-2- aminobenzoic acid of SHZTN. Samples were prepared using a methanol precipitation method and the internal standards lannaconitine and p-hydroxybenzoic acid were added. The method validation was conducted according to the guidelines of the Pharmacopoeia of China. A good linear range was obtained in the range of 1-2,000 ng/ml. The intra-day and inter-day precisions were less than 14.7%, and the accuracy range of all the analytes was -10.5-9.3%. The recovery of each analyte was over 95.5%, and matrix effects can be neglected. After a single dose of 20 mg/kg SHZTN, the area under the curve and peak concentration of the five active ingredients were significantly increased by transdermal compared with oral administration, which indicated the high bioavailability of SHZTN. The time to peak concentration of all compounds was <3.4 h, and the half-life was <15.4 h, which indicated that the five compounds have the best absorption and rapid elimination. The method was successfully developed and applied to the pharmacokinetic study of SHZTN.
    Language English
    Publishing date 2022-10-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 632848-9
    ISSN 1099-0801 ; 0269-3879
    ISSN (online) 1099-0801
    ISSN 0269-3879
    DOI 10.1002/bmc.5533
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  5. Article ; Online: Mechanism of Shuang-Huang-Lian Oral Liquid for Treatment of Mycoplasmal Pneumonia in Children on Network Pharmacology.

    Shi, Ling / Wu, Qi-Guo / Zhang, Ju-Cheng / Yang, Guang-Ming / Liu, Wei / Wang, Ze-Feng

    Combinatorial chemistry & high throughput screening

    2020  Volume 23, Issue 9, Page(s) 955–971

    Abstract: ... system immune damage, and mixed infection in children. The pathogenesis is still unclear. Shuang-Huang-Lian (SHL ...

    Abstract Background and objective: Mycoplasmal pneumonia (MP) can lead to inflammation, multiple system immune damage, and mixed infection in children. The pathogenesis is still unclear. Shuang-Huang-Lian (SHL) oral liquid can treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. However, our current understanding of the molecular mechanisms supporting its clinical application still lags behind due to the lack of researches. It is difficult to understand the overall sensitization mechanism of SHL oral liquid. The purpose is to explain the mechanism of action of drugs in this study, which is useful to ensure the safety of medication for children.
    Methods: The therapeutic mechanism of SHL oral liquid was investigated by a system pharmacology approach integrating drug-likeness evaluation, oral bioavailability prediction, ADMET, protein-protein interaction worknet, Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes database pathway performance, C-T-P network construction and molecular docking.
    Results: A total of 18 active ingredients contained in SHL oral liquid and 53 major proteins were screened out as effective players in the treatment of M. pneumoniae disease through some related pathways and molecular docking. The majority of targets, hubs and pathways were highly related to anti-mycoplasma therapy, immunity and inflammation process.
    Conclusion: This study shows that the anti-bacterial effect of SHL oral liquid has multicomponent, multi-target and multi-pathway phenomena. The proposed approach may provide a feasible tool to clarify the mechanism of traditional Chinese medicines and further develop their therapeutic potentials.
    MeSH term(s) Anti-Infective Agents/chemistry ; Anti-Infective Agents/pharmacokinetics ; Databases, Genetic ; Drug Evaluation, Preclinical ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/pharmacokinetics ; Gene Expression Regulation/drug effects ; Gene Ontology ; Humans ; Medicine, Chinese Traditional ; Molecular Docking Simulation ; Pneumonia, Mycoplasma/drug therapy ; Protein Binding ; Respiratory Tract Infections/drug therapy ; Signal Transduction ; Small Molecule Libraries/chemistry ; Small Molecule Libraries/pharmacology
    Chemical Substances Anti-Infective Agents ; Drugs, Chinese Herbal ; Small Molecule Libraries ; shuang-huang-lian
    Language English
    Publishing date 2020-05-14
    Publishing country United Arab Emirates
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2064785-2
    ISSN 1875-5402 ; 1386-2073
    ISSN (online) 1875-5402
    ISSN 1386-2073
    DOI 10.2174/1386207323666200514073428
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 5577: Show issues Location:
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  6. Article ; Online: An on-line analytical approach for detecting haptens in Shuang-huang-lian powder injection.

    Wang, Hong / Zhang, Xiaotian / Wang, Daidong / Sun, Hongyang / Lan, Yuexiang / Jiang, Haixiu / Chen, Shizhong / Lin, Zongtao

    Journal of chromatography. A

    2017  Volume 1513, Page(s) 126–139

    Abstract: Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI ...

    Abstract Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI) frequently used in the clinical treatment of faucitis, bronchitis, and other viral and bacterial infections of upper respiratory tract. However, its allergenic reactions, being the main adverse effects (AEs) of SHLPI, have been a serious problem of its clinical safety. This problem has not been solved due to short of methods for detecting haptens in complex TCMIs. In this study, an on-line high-performance liquid chromatography-diode-array detector-mass spectrometry combined with bovine serum albumin-fluorescence detector (HPLC-DAD-MS-BSA-FLD) system was established for the first time, validated and applied for identification of haptens in SHLPI. Fourteen of 35 identified compounds showed BSA binding activity, and they were six flavonoids, six caffeoylquinic acids (CQAs), and two phenylethanoid glycosides. The structure-activity relationships of 10 active components were studied, and their ability of sensitization together with that of two CQAs were further verified by ELISA assay. It was found that 10 compounds had sensitization, and flavonoids showed stronger sensitizability than CQAs while the diCQAs were slightly stronger than caffeoylquinic acids. The system was validated using 3-CQA as a positive control, and was proved to have good reproducibility, stability, precision (RSD<0.1%) and linearity (R
    Language English
    Publishing date 2017-09-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2017.07.031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Screening anaphylactoid components of Shuang Huang Lian Injection by analyzing spectrum-effect relationships coupled with UPLC-TOF-MS.

    Feng, Yufei / Jing, Zhongxu / Li, Yongji / Lv, Shaowa / Li, Weinan / Cai, Guofeng / Yang, Danqi / Wang, Yanhong

    Biomedical chromatography : BMC

    2018  Volume 33, Issue 2, Page(s) e4376

    Abstract: Shuang Huang Lian Injection (SHLI) has been used in China for over 30 years as an effective and ...

    Abstract Shuang Huang Lian Injection (SHLI) has been used in China for over 30 years as an effective and widely used Chinese herbal prescription to treat acute respiratory infectious. SHLI has, however, caused many severe anaphylactoid reactions. It is important to identify the potential anaphylactoid components of SHLI. Spectrum-effect relationships were used to explore potentially anaphylactoid components. Based on the original herbal formula, honeysuckle, Fructus Forsythiae and Radix Scutellariae extracts were prepared and combined in appropriate proportions. The preparations were then injected into the caudal vein of rats to obtain in vivo serum samples for pharmacological evaluation and fingerprint analysis. The release rate of β-hexosaminidase from RBL-2H
    MeSH term(s) Anaphylaxis ; Animals ; Antigens, Plant/analysis ; Antigens, Plant/chemistry ; Cell Line ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/analysis ; Drugs, Chinese Herbal/chemistry ; Female ; Hexosaminidase B/blood ; Hexosaminidase B/metabolism ; Histamine/blood ; Lonicera/chemistry ; Male ; Mass Spectrometry ; Rats ; Rats, Wistar
    Chemical Substances Antigens, Plant ; Drugs, Chinese Herbal ; shuang-huang-lian ; Histamine (820484N8I3) ; Hexosaminidase B (EC 3.2.1.52)
    Language English
    Publishing date 2018-11-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 632848-9
    ISSN 1099-0801 ; 0269-3879
    ISSN (online) 1099-0801
    ISSN 0269-3879
    DOI 10.1002/bmc.4376
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  8. Article ; Online: Bioactive compounds of Shuang-Huang-Lian prescription and an insight into its binding mechanism by β

    Wang, Jing / Li, Fengwu / Zeng, Kaizhu / Li, Qian / Zhao, Xinfeng / Zheng, Xiaohui

    Journal of separation science

    2017  Volume 40, Issue 22, Page(s) 4357–4365

    Abstract: Owing to the promising clinical efficacy and relatively simple composition, Shuang-Huang-Lian ...

    Abstract Owing to the promising clinical efficacy and relatively simple composition, Shuang-Huang-Lian prescription is widely prescribed for the treatment of acute upper respiratory tract infection and acute bronchitis in practice. This necessitates the understanding of the bioactive compounds of the prescription and their binding mechanism to β
    MeSH term(s) Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal/chemistry ; Molecular Docking Simulation ; Phytochemicals/analysis ; Receptors, Adrenergic/chemistry
    Chemical Substances Drugs, Chinese Herbal ; Phytochemicals ; Receptors, Adrenergic ; shuang-huang-lian
    Language English
    Publishing date 2017-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2047990-6
    ISSN 1615-9314 ; 1615-9306
    ISSN (online) 1615-9314
    ISSN 1615-9306
    DOI 10.1002/jssc.201700522
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: An on-line analytical approach for detecting haptens in Shuang-huang-lian powder injection

    Wang, Hong / Xiaotian Zhang / Daidong Wang / Hongyang Sun / Yuexiang Lan / Haixiu Jiang / Shizhong Chen / Zongtao Lin

    Journal of chromatography. 2017,

    2017  

    Abstract: Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI ...

    Abstract Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI) frequently used in the clinical treatment of faucitis, bronchitis, and other viral and bacterial infections of upper respiratory tract. However, its allergenic reactions, being the main adverse effects (AEs) of SHLPI, have been a serious problem of its clinical safety. This problem has not been solved due to short of methods for detecting haptens in complex TCMIs. In this study, an on-line high-performance liquid chromatography-diode-array detector-mass spectrometry combined with bovine serum albumin-fluorescence detector (HPLC-DAD-MS-BSA-FLD) system was established for the first time, validated and applied for identification of haptens in SHLPI. Fourteen of 35 identified compounds showed BSA binding activity, and they were six flavonoids, six caffeoylquinic acids (CQAs), and two phenylethanoid glycosides. The structure-activity relationships of 10 active components were studied, and their ability of sensitization together with that of two CQAs were further verified by ELISA assay. It was found that 10 compounds had sensitization, and flavonoids showed stronger sensitizability than CQAs while the diCQAs were slightly stronger than caffeoylquinic acids. The system was validated using 3-CQA as a positive control, and was proved to have good reproducibility, stability, precision (RSD<0.1%) and linearity (R2>0.9993). This online system is fast, sensitive and efficient for screening haptens in traditional Chinese medicine injection (TCMI), provides a new approach to reveal the chemical basis of haptens in TCMIs.
    Keywords Oriental traditional medicine ; adverse effects ; allergenicity ; bacterial infections ; blood serum ; bronchitis ; cattle ; chlorogenic acid ; chromatography ; enzyme-linked immunosorbent assay ; flavonoids ; glycosides ; haptens ; respiratory system ; screening ; spectroscopy ; structure-activity relationships
    Language English
    Size p. .
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 218139-3
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    DOI 10.1016/j.chroma.2017.07.031
    Database NAL-Catalogue (AGRICOLA)

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  10. Book: Shuang shuang zhong wen jiao cai

    Wang, Shuangshuang

    = Chinese language and culture course

    2008  

    Author's details Wang Shuangshuang bian zhu
    Language Chinese
    Size 38 S
    Publisher Beijing da xue chu ban she
    Publishing place Beijing
    Document type Book
    Accompanying material 1 CD, lianxi ce dan ke 20 S., lianxi ce shuang ke 16 S.
    ISBN 7301086954 ; 9787301086957
    Database Former special subject collection: coastal and deep sea fishing

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