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  1. Article: Antibodies and HIV: new evidence. Interview with Ruth Ruprecht, M.D., Ph.D. Interview by David Scondras and John S. James.

    Ruprecht, R

    AIDS treatment news

    2001  , Issue 365, Page(s) 2–4

    MeSH term(s) Animals ; Female ; HIV Antibodies/biosynthesis ; HIV Antibodies/immunology ; HIV Infections/immunology ; HIV Infections/therapy ; Humans ; Immunoglobulin G/immunology ; Immunotherapy/methods ; Infectious Disease Transmission, Vertical/prevention & control ; Macaca mulatta ; Pregnancy ; Pregnancy Complications, Infectious
    Chemical Substances HIV Antibodies ; Immunoglobulin G
    Language English
    Publishing date 2001-05-25
    Publishing country United States
    Document type Interview ; Newspaper Article
    ZDB-ID 1329805-7
    ISSN 1052-4207
    ISSN 1052-4207
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4477: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Anti-HIV Passive Immunization: New Weapons in the Arsenal.

    Ruprecht, Ruth M

    Trends in microbiology

    2017  Volume 25, Issue 12, Page(s) 954–956

    Abstract: Anti-HIV passive immunization with human neutralizing monoclonal antibodies (nmAbs) has made exciting gains: (i) identification of the HIV envelope V2 apex as a new in vivo protective epitope, (ii) a novel clade C SHIV for challenge studies, and (iii) a ... ...

    Abstract Anti-HIV passive immunization with human neutralizing monoclonal antibodies (nmAbs) has made exciting gains: (i) identification of the HIV envelope V2 apex as a new in vivo protective epitope, (ii) a novel clade C SHIV for challenge studies, and (iii) a highly protective, trispecific nmAb. Potent, broad-spectrum protection by nmAbs holds promise.
    MeSH term(s) Antibodies, Monoclonal ; Antibodies, Neutralizing ; HIV-1/immunology ; Humans ; Immunization, Passive ; Neutralization Tests ; Weapons
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Neutralizing
    Language English
    Publishing date 2017-10-30
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2017.10.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3738: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Immunoglobulin M: An Ancient Antiviral Weapon - Rediscovered.

    Gong, Siqi / Ruprecht, Ruth M

    Frontiers in immunology

    2020  Volume 11, Page(s) 1943

    Abstract: Recent discoveries have shed new light onto immunoglobulin M (IgM), an ancient antibody class ...

    Abstract Recent discoveries have shed new light onto immunoglobulin M (IgM), an ancient antibody class preserved throughout evolution in all vertebrates. First, IgM - long thought to be a perfect pentamer - was shown to be asymmetric, resembling a quasi-hexamer missing one monomer and containing a gap. Second, this gap allows IgM to serve as carrier of a specific host protein, apoptosis inhibitor of macrophages (AIM), which is released to promote removal of dead-cell debris, cancer cells, or pathogens. Third, recombinant IgM delivered mucosally by passive immunization gave proof-of-concept that this antibody class can prevent mucosal simian-human immunodeficiency virus transmission in non-human primates. Finally, IgM's role in adaptive immunity goes beyond being only a first defender to respond to pathogen invasion, as long-lived IgM plasma cells have been observed predominantly residing in the spleen. In fact, IgM produced by such cells contained somatic hypermutations and was linked to protection against lethal influenza virus challenge in murine models. Importantly, such long-lived IgM plasma cells had been induced by immunization 1 year before challenge. Together, new data on IgM function raise the possibility that vaccine strategies aimed at preventing virus acquisition could include this ancient weapon.
    MeSH term(s) Adaptive Immunity ; Animals ; Host-Pathogen Interactions ; Humans ; Immunity, Mucosal ; Immunization ; Immunogenicity, Vaccine ; Immunoglobulin M/immunology ; Immunoglobulin M/metabolism ; Plasma Cells/immunology ; Plasma Cells/virology ; Signal Transduction ; Viral Vaccines/administration & dosage ; Virus Diseases/immunology ; Virus Diseases/metabolism ; Virus Diseases/prevention & control ; Virus Diseases/virology ; Viruses/immunology ; Viruses/pathogenicity
    Chemical Substances Immunoglobulin M ; Viral Vaccines
    Keywords covid19
    Language English
    Publishing date 2020-08-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2020.01943
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Elucidating Vaccine Trafficking Mechanisms using Multimodal Imaging.

    Hartwell, Brittany L / Martin, Jacob / Chang, Jason Y H / Kumarapperuma, Sidath C / Ruprecht, Ruth M / Irvine, Darrell J

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue 29 Suppl 1, Page(s) 1068

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.547
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Antibody Light Chains: Key to Increased Monoclonal Antibody Yields in Expi293 Cells?

    Gong, Siqi / Gautam, Seijal / Coneglio, Joshua D / Scinto, Hanna B / Ruprecht, Ruth M

    Antibodies (Basel, Switzerland)

    2022  Volume 11, Issue 2

    Abstract: When constructing isogenic recombinant IgM-IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, ... ...

    Abstract When constructing isogenic recombinant IgM-IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, IgM yields were low but increased by logs-up to 20,000 X-by using λ chains instead. Switching light chains did not alter epitope specificity. For dimeric IgA2, optimal expression involved pairing with λ chains, whereas light-chain preference varied for other immunoglobulin classes. In summary, recombinant IgM production can be drastically increased by using λ chains, an important finding in the use of IgM for mucosal immunoprophylaxis.
    Language English
    Publishing date 2022-05-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661514-9
    ISSN 2073-4468 ; 2073-4468
    ISSN (online) 2073-4468
    ISSN 2073-4468
    DOI 10.3390/antib11020037
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Mucosal IgA Responses: Damaged in Established HIV Infection-Yet, Effective Weapon against HIV Transmission.

    Kulkarni, Viraj / Ruprecht, Ruth M

    Frontiers in immunology

    2017  Volume 8, Page(s) 1581

    Abstract: HIV infection not only destroys ... ...

    Abstract HIV infection not only destroys CD4
    Language English
    Publishing date 2017
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2017.01581
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Anti-HIV Passive Immunization: New Weapons in the Arsenal

    Ruprecht, Ruth M

    Trends in microbiology. 2017 Dec., v. 25, no. 12

    2017  

    Abstract: Anti-HIV passive immunization with human neutralizing monoclonal antibodies (nmAbs) has made exciting gains: (i) identification of the HIV envelope V2 apex as a new in vivo protective epitope, (ii) a novel clade C SHIV for challenge studies, and (iii) a ... ...

    Abstract Anti-HIV passive immunization with human neutralizing monoclonal antibodies (nmAbs) has made exciting gains: (i) identification of the HIV envelope V2 apex as a new in vivo protective epitope, (ii) a novel clade C SHIV for challenge studies, and (iii) a highly protective, trispecific nmAb. Potent, broad-spectrum protection by nmAbs holds promise.
    Keywords Human immunodeficiency virus ; antiretroviral agents ; epitopes ; humans ; immunization ; monoclonal antibodies ; neutralization
    Language English
    Dates of publication 2017-12
    Size p. 954-956.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2017.10.006
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2005/714: Show issues

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  8. Article ; Online: Antibody-mediated immune exclusion of HIV.

    Ruprecht, Ruth M / Lakhashe, Samir K

    Current opinion in HIV and AIDS

    2017  Volume 12, Issue 3, Page(s) 222–228

    Abstract: Purpose of review: Although approximately 90% of all HIV transmissions in humans occur through mucosal contact, the induction of mucosal anti-HIV immune responses has remained understudied. Here we summarize data demonstrating the powerful protection ... ...

    Abstract Purpose of review: Although approximately 90% of all HIV transmissions in humans occur through mucosal contact, the induction of mucosal anti-HIV immune responses has remained understudied. Here we summarize data demonstrating the powerful protection that is achievable at mucosal frontlines through virus-specific mucosal IgA alone or combined with IgG.
    Recent findings: Passive immunization with different monoclonal antibody subclasses but identical epitope specificity (the conserved V3-loop crown of HIV gp120) has revealed that the dimeric IgA1 (dIgA1) form with its open hinge can prevent simian-human immunodeficiency virus (SHIV) acquisition in rhesus macaques at a higher rate than dIgA2. Both dIgAs neutralized the challenge SHIV equally well. Protection was linked to better virion capture and inhibition of cell-free virus transcytosis by dIgA1. Synergistic interactions at the mucosal level between the IgG1 and dIgA2 versions of this monoclonal antibody yielded complete protection. Active vaccine strategies designed to induce mucosal IgA and systemic/mucosal IgG have given promising data.
    Summary: This review seeks to highlight the importance of mucosal IgAs in preventing virus acquisition. Passive immunization gave proof-of-concept for immune exclusion by mucosally administered monoclonal dIgAs. Unanswered questions remain regarding the interplay between mucosal IgA and other host immune defenses, including their induction with active immunization.
    MeSH term(s) Animals ; HIV Antibodies/immunology ; HIV Infections/immunology ; HIV Infections/virology ; HIV-1/immunology ; HIV-1/physiology ; Humans ; Immunity, Mucosal
    Chemical Substances HIV Antibodies
    Language English
    Publishing date 2017-05
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2502511-9
    ISSN 1746-6318 ; 1746-630X
    ISSN (online) 1746-6318
    ISSN 1746-630X
    DOI 10.1097/COH.0000000000000369
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6812: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  9. Article: Mucosal Antibodies: Defending Epithelial Barriers against HIV-1 Invasion.

    Ruprecht, Ruth M / Marasini, Bishal / Thippeshappa, Rajesh

    Vaccines

    2019  Volume 7, Issue 4

    Abstract: The power of mucosal anti-HIV-1 envelope immunoglobulins (Igs) to block virus transmission is underappreciated. We used passive immunization, a classical tool to unequivocally prove whether antibodies are protective. We mucosally instilled recombinant ... ...

    Abstract The power of mucosal anti-HIV-1 envelope immunoglobulins (Igs) to block virus transmission is underappreciated. We used passive immunization, a classical tool to unequivocally prove whether antibodies are protective. We mucosally instilled recombinant neutralizing monoclonal antibodies (nmAbs) of different Ig classes in rhesus macaques (RMs) followed by mucosal simian-human immunodeficiency virus (SHIV) challenge. We gave anti-HIV-1 IgM, IgG, and dimeric IgA (dIgA) versions of the same human nmAb, HGN194 that targets the conserved V3 loop crown. Surprisingly, dIgA1 with its wide-open, flat hinge protected 83% of the RMs against intrarectal R5-tropic SHIV-1157ipEL-p challenge, whereas dIgA2, with its narrow hinge, only protected 17% of the animals-despite identical epitope specificities and in vitro neutralization curves of the two dIgA isotypes (Watkins et al.,
    Language English
    Publishing date 2019-11-23
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines7040194
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Passive immunization with human neutralizing monoclonal antibodies against HIV-1 in macaque models: experimental approaches.

    Ruprecht, Ruth M

    Methods in molecular biology (Clifton, N.J.)

    2009  Volume 525, Page(s) 559–66, xiv

    Abstract: After more than 20 years of intense research, a safe and effective vaccine against HIV-1/AIDS has not been developed. Passive immunization has been used as a tool to demonstrate the role of neutralizing antibodies in conferring protection against HIV-1 ... ...

    Abstract After more than 20 years of intense research, a safe and effective vaccine against HIV-1/AIDS has not been developed. Passive immunization has been used as a tool to demonstrate the role of neutralizing antibodies in conferring protection against HIV-1 challenge in chimpanzees. Because these animals are endangered and studies are difficult to conduct with this species, chimeric viruses, termed simian-human immunodeficiency viruses (SHIVs), have been generated that encode the HIV-1 envelope gene in the backbone of the simian immunodeficiency virus (SIV). SHIVs replicate in several macaque species and can induce AIDS in these animals. Passive immunization with human neutralizing monoclonal antibodies (nmAbs) against HIV-1 has protected rhesus macaques from SHIV infection and provided proof-of-concept of the protective effects of neutralizing antibodies. At the same time, human nmAbs can be evaluated for safety and efficacy in the SHIV/macaque model as therapeutic modalities in their own right for prevention, post-exposure prophylaxis, or possibly therapeutic use. Experimental details are provided for testing human nmAbs in infant rhesus monkeys, which allows testing without the need to generate large amounts of nmAbs.
    MeSH term(s) Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/therapeutic use ; Antibodies, Viral/immunology ; Blood Specimen Collection ; HIV-1/immunology ; Humans ; Immunization, Passive/methods ; Macaca/blood ; Macaca/immunology ; Macaca/virology ; Models, Animal ; Neutralization Tests ; RNA, Viral/blood ; Simian Immunodeficiency Virus/immunology ; Viral Load
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Viral ; RNA, Viral
    Language English
    Publishing date 2009
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1007/978-1-59745-554-1_31
    Database MEDical Literature Analysis and Retrieval System OnLINE

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