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Book: Clinical Laboratory Management

Allen, Timothy C / Wolk, Donna M / Baselski, Vickie S / Church, Deirdre L / Karcher, Donald S / Lewis, Michael R / Linscott, Andrea J / Poulter, Melinda D / Procop, Gary W / Weissfeld, Alice S

2024

Author's details	Lynne S. Garcia, L&G Asssociates, Santa Monica, California Timothy C. Allen, Corewell Health, Royal Oak, Michigan Vickie S. Baselski, University of Tennessee Health Science Center, Memphis, Tennessee Deirdre L. Church, University of Calgary, Calgary, Alberta, Canada Donald S. Karcher, George Washington University, Washington, DC Michael R. Lewis, Banner MD Anderson Cancer Center, Gilbert, Arizona Andrea J. Linscott, Ochsner Medical Center, New Orleans, Louisiana Melinda D. Poulter, University of Virginia, Charlottesville, Virginia Gary W. Procop, American Board of Pathology, Tampa, Florida Alice S. Weissfeld, Microbiology Specialists, Inc., Houston, Texas Donna M. Wolk, Geisinger, Diagnostic Medicine Institute, Department of Laboratory Medicine, Danville, Pennsylvania
Language	English
Size	816 p.
Edition	3
Publisher	Wiley
Document type	Book
Note	PDA Manuell_25
Format	216 x 283 x 36
ISBN	9781683673910 ; 1683673913
Database	PDA

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Article ; Online: The Btk inhibitor AB-95-LH34 potently inhibits atherosclerotic plaque-induced thrombus formation and platelet procoagulant activity.

Smith, Christopher W / Harbi, Maan H / Garcia-Quintanilla, Lourdes / Rookes, Kieran / Brown, Helena / Poulter, Natalie S / Watson, Steve P / Nicolson, Phillip L R / Thomas, Mark R

Journal of thrombosis and haemostasis : JTH

2022 Volume 20, Issue 12, Page(s) 2939–2952

Abstract: Background: New antithrombotic therapies with less effect on bleeding are needed for coronary artery disease. The Btk inhibitor ibrutinib blocks atherosclerotic plaque-mediated thrombus formation. However, it is associated with increased bleeding, ... ...

Abstract	Background: New antithrombotic therapies with less effect on bleeding are needed for coronary artery disease. The Btk inhibitor ibrutinib blocks atherosclerotic plaque-mediated thrombus formation. However, it is associated with increased bleeding, possibly due to non-Btk-mediated effects. Btk-deficient patients do not have bleeding issues, suggesting selective Btk inhibition as a promising antithrombotic strategy. Objectives: To compare the antithrombotic effects of the highly selective Btk inhibitor AB-95-LH34 (LH34) with ibrutinib. Methods: Glycoprotein VI and G-protein coupled receptor-mediated platelet function and signaling were analyzed in healthy human donor platelets by lumi-aggregometry, flow adhesion, and western blot following 1 h treatment with inhibitors in vitro. Results: LH34 showed similar inhibition of Btk-Y223 phosphorylation as ibrutinib, but had no off-target inhibition of Src-Y418 phosphorylation. Similar dose-dependent inhibition of aggregation to atherosclerotic plaque material was observed for both. However, in response to Horm collagen, which also binds integrin α2β1, LH34 exhibited less marked inhibition than ibrutinib. Both LH34 and ibrutinib inhibited platelet adhesion and aggregation to plaque material at arterial shear. Ibrutinib demonstrated the most potent effect, with complete blockade at high concentrations. Platelet activation (P-selectin) and procoagulant activity (phosphatidylserine exposure) in thrombi were inhibited by LH34 and completely blocked by ibrutinib at high concentrations. Furthermore, plaque-induced thrombin generation was reduced by higher concentrations of LH34 and ibrutinib. Conclusions: LH34 potently inhibits atherosclerotic plaque-induced thrombus formation and procoagulant platelet activity in vitro, with less off-target inhibition of Src than ibrutinib, suggesting it is a promising antiplatelet therapy with the potential for reduced bleeding side effects.
MeSH term(s)	Humans ; Plaque, Atherosclerotic/complications ; Fibrinolytic Agents/therapeutic use ; Blood Platelets/metabolism ; Platelet Activation ; Protein Kinase Inhibitors/adverse effects ; Thrombosis/drug therapy ; Atherosclerosis/complications ; Hemorrhage/chemically induced ; Platelet Aggregation ; Platelet Aggregation Inhibitors/therapeutic use
Chemical Substances	Fibrinolytic Agents ; Protein Kinase Inhibitors ; Platelet Aggregation Inhibitors
Language	English
Publishing date	2022-10-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2112661-6
ISSN	1538-7836 ; 1538-7933
ISSN (online)	1538-7836
ISSN	1538-7933
DOI	10.1111/jth.15899
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Zs.A 5805: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Prehospital Activation of the Cardiac Catheterization Laboratory in ST-Segment-Elevation Myocardial Infarction for Primary Percutaneous Coronary Intervention.

Savage, Michael L / Hay, Karen / Vollbon, William / Doan, Tan / Murdoch, Dale J / Hammett, Christopher / Poulter, Rohan / Walters, Darren L / Denman, Russell / Ranasinghe, Isuru / Raffel, Owen Christopher

Journal of the American Heart Association

2023 Volume 12, Issue 14, Page(s) e029346

Abstract: Background Prehospital activation of the cardiac catheter laboratory is associated with significant improvements in ST-segment-elevation myocardial infarction (STEMI) performance measures. However, there are equivocal data, particularly within Australia, ...

Abstract	Background Prehospital activation of the cardiac catheter laboratory is associated with significant improvements in ST-segment-elevation myocardial infarction (STEMI) performance measures. However, there are equivocal data, particularly within Australia, regarding its influence on mortality. We assessed the association of prehospital activation on performance measures and mortality in patients with STEMI treated with primary percutaneous coronary intervention from the Queensland Cardiac Outcomes Registry (QCOR). Methods and Results Consecutive ambulance-transported patients with STEMI treated with primary percutaneous coronary intervention were analyzed from January 1, 2017 to December 31, 2020 from the QCOR. The total and direct effects of prehospital activation on the primary outcomes (30-day and 1-year cardiovascular mortality) were estimated using logistic regression analyses. Secondary outcomes were STEMI performance measures. Among 2498 patients (mean age: 62.2±12.4 years; 79.2% male), 73% underwent prehospital activation. Median door-to-balloon time (34 minutes [26-46] versus 86 minutes [68-113];
MeSH term(s)	Humans ; Male ; Middle Aged ; Aged ; Female ; ST Elevation Myocardial Infarction/diagnosis ; ST Elevation Myocardial Infarction/surgery ; Emergency Medical Services ; Angioplasty, Balloon, Coronary ; Myocardial Infarction ; Percutaneous Coronary Intervention/adverse effects ; Electrocardiography ; Cardiac Catheterization
Language	English
Publishing date	2023-07-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2653953-6
ISSN	2047-9980 ; 2047-9980
ISSN (online)	2047-9980
ISSN	2047-9980
DOI	10.1161/JAHA.122.029346
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Article: Proportional increase in the theta carrying lymphocytes in peripheral lymphoid tissue following treatment with cyclophosphamide.

Poulter, L W / Turk, J L

Nature: New biology

2008 Volume 238, Issue 79, Page(s) 17–18

MeSH term(s)	Cyclophosphamide/therapeutic use ; Globins/metabolism ; Humans ; Immunosuppressive Agents/therapeutic use ; Lymphocytes/drug effects ; Lymphocytes/immunology ; Lymphocytes/physiology ; Lymphoid Tissue/drug effects ; Lymphoid Tissue/immunology ; Lymphoid Tissue/physiology
Chemical Substances	Immunosuppressive Agents ; Cyclophosphamide (8N3DW7272P) ; Globins (9004-22-2)
Language	English
Publishing date	2008-07-24
Publishing country	England
Document type	Letter
ZDB-ID	120715-5
ISSN	0090-0028 ; 0369-4887
ISSN	0090-0028 ; 0369-4887
DOI	10.1038/newbio238017a0
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Article: May Measurement Month 2018: an analysis of blood pressure screening results from Benin

Houehanou, Corine / Sonou, Arnaud / Adjagba, Philippe / Dohou, Hugues / Hounkponou, Murielle / Kpolédji, Gwladys / Saka, Dominique / Assogba, Gildas / Assani, Salimatou / Wang, Wei / Beaney, Thomas / Poulter, Neil R / Codjo, Léopold / Houenassi, Martin D

European heart journal supplements : journal of the European Society of Cardiology

2022 Volume 24, Issue Suppl F, Page(s) F9–F11

Abstract: Hypertension is the strongest cardiovascular risk factor worldwide. May Measurement Month (MMM) is an international campaign for blood pressure (BP) screening initiated by the International Society of Hypertension. This work aims to estimate the ... ...

Abstract	Hypertension is the strongest cardiovascular risk factor worldwide. May Measurement Month (MMM) is an international campaign for blood pressure (BP) screening initiated by the International Society of Hypertension. This work aims to estimate the proportion and the levels of awareness, treatment, and control of hypertension in participants of the MMM survey in Benin in 2018. A cross-sectional survey focused on people aged ≥18 years was conducted in May 2018 in nine rural and urban areas in Benin. A sampling of volunteers was done. BP was measured following the MMM protocol. Hypertension was defined as a systolic BP ≥140 mm Hg and/or a diastolic BP ≥90 mm Hg (mean of the second and third readings) and/or taking antihypertensive medication. Linear regression was used to identify BP associations. A total of 2035 people were screened, including 55.9% women. The mean age was 44.2 ± 15.9 years. The percentage with hypertension was 35.4%. Of 721 participants with hypertension, 56.2% were aware of their diagnosis, 39.7% were on antihypertensive medication, and 13.6% were controlled (<140/90 mmHg). The results confirm the significant proportion of hypertension in Benin. Education programs on risk factors, early detection, and better management strategies should be developed.
Language	English
Publishing date	2022-10-07
Publishing country	England
Document type	Journal Article
ZDB-ID	1463769-8
ISSN	1554-2815 ; 1520-765X
ISSN (online)	1554-2815
ISSN	1520-765X
DOI	10.1093/eurheartjsupp/suac039
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Zs.A 1563 -Suppl.-: Show issues

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Article: May Measurement Month 2017-2019: an analysis of blood pressure screening results from Dominican Republic

Valdez-Tiburcio, Osiris / Gonzalez-Medina, Angel / Valdez-Valoy, Laura / Santillan-Pascual, Marcos / Baez-Noyer, Nelson / Diaz-Montero, Belianix / Wang, Wei / Beaney, Thomas / Poulter, Neil R

European heart journal supplements : journal of the European Society of Cardiology

2022 Volume 24, Issue Suppl F, Page(s) F12–F15

Abstract: Arterial hypertension is the main risk factor that contributes to cardiovascular disease and represents a leading cause of morbidity and mortality globally. May Measurement Month (MMM) is a global screening campaign with the aim of improving awareness of ...

Abstract	Arterial hypertension is the main risk factor that contributes to cardiovascular disease and represents a leading cause of morbidity and mortality globally. May Measurement Month (MMM) is a global screening campaign with the aim of improving awareness of hypertension at the individual and population level, an initiative that has been supported in the Dominican Republic (DR) since 2017. Adults (≥18 years) were recruited by sampling in different places in the DR, three blood pressure (BP) readings were performed per participant, and data on risk factors and comorbidities were collected. Hypertension was defined as systolic BP ≥140 mm Hg, diastolic BP ≥ 90 mm Hg (mean of second and third readings), and/or taking antihypertensive medication. Multiple imputation was used to estimate participants' mean BP when three readings were not available. Of 3693 participants, 2134 (57.8%) had hypertension, of whom 1646 (77.1%) were taking medication, but only 38.6% of those on treatment had their BP under control(<140/90 mmHg). The remaining 61.4% of the participants received inadequate treatment. A total of 66% of treated patients were taking a single antihypertensive drug. MMM provides an important platform for the standardized compilation of BP data and the creation of awareness of hypertension in the DR and other nations of the world. The data generated from the 2017-2019 MMM campaigns highlight the importance of adequate detection, knowledge, and control of BP.
Language	English
Publishing date	2022-10-07
Publishing country	England
Document type	Journal Article
ZDB-ID	1463769-8
ISSN	1554-2815 ; 1520-765X
ISSN (online)	1554-2815
ISSN	1520-765X
DOI	10.1093/eurheartjsupp/suac040
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Article ; Online: Missense mutation of MAL causes a rare leukodystrophy similar to Pelizaeus-Merzbacher disease.

Elpidorou, Marilena / Poulter, James A / Szymanska, Katarzyna / Baron, Wia / Junger, Katrin / Boldt, Karsten / Ueffing, Marius / Green, Lydia / Livingston, John H / Sheridan, Eammon G / Johnson, Colin A

European journal of human genetics : EJHG

2022 Volume 30, Issue 7, Page(s) 860–864

Abstract: Leukodystrophies are a heterogenous group of genetic disorders, characterised by abnormal development of cerebral white matter. Pelizaeus-Merzbacher disease is caused by mutations in PLP1, encoding major myelin-resident protein required for myelin sheath ...

Abstract	Leukodystrophies are a heterogenous group of genetic disorders, characterised by abnormal development of cerebral white matter. Pelizaeus-Merzbacher disease is caused by mutations in PLP1, encoding major myelin-resident protein required for myelin sheath assembly. We report a missense variant p.(Ala109Asp) in MAL as causative for a rare, hypomyelinating leukodystrophy similar to Pelizaeus-Merzbacher disease. MAL encodes a membrane proteolipid that directly interacts with PLP1, ensuring correct distribution during myelin assembly. In contrast to wild-type MAL, mutant MAL was retained in the endoplasmic reticulum but was released following treatment with 4-phenylbutyrate. Proximity-dependent identification of wild-type MAL interactants implicated post-Golgi vesicle-mediated protein transport and protein localisation to membranes, whereas mutant MAL interactants suggested unfolded protein responses. Our results suggest that mislocalisation of MAL affects PLP1 distribution, consistent with known pathomechanisms for hypomyelinating leukodystrophies.
MeSH term(s)	Humans ; Mutation ; Mutation, Missense ; Myelin Proteolipid Protein/genetics ; Myelin Proteolipid Protein/metabolism ; Neurodegenerative Diseases ; Pelizaeus-Merzbacher Disease/genetics ; Protein Transport
Chemical Substances	Myelin Proteolipid Protein
Language	English
Publishing date	2022-02-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1141470-4
ISSN	1476-5438 ; 1018-4813
ISSN (online)	1476-5438
ISSN	1018-4813
DOI	10.1038/s41431-022-01050-9
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Zs.A 3589: Show issues

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Article ; Online: A missense variant in specificity protein 6 (SP6) is associated with amelogenesis imperfecta.

Smith, Claire E L / Whitehouse, Laura L E / Poulter, James A / Wilkinson Hewitt, Laura / Nadat, Fatima / Jackson, Brian R / Manfield, Iain W / Edwards, Thomas A / Rodd, Helen D / Inglehearn, Chris F / Mighell, Alan J

Human molecular genetics

2020 Volume 29, Issue 9, Page(s) 1417–1425

Abstract: Amelogenesis is the process of enamel formation. For amelogenesis to proceed, the cells of the inner enamel epithelium (IEE) must first proliferate and then differentiate into the enamel-producing ameloblasts. Amelogenesis imperfecta (AI) is a ... ...

Abstract	Amelogenesis is the process of enamel formation. For amelogenesis to proceed, the cells of the inner enamel epithelium (IEE) must first proliferate and then differentiate into the enamel-producing ameloblasts. Amelogenesis imperfecta (AI) is a heterogeneous group of genetic conditions that result in defective or absent tooth enamel. We identified a 2 bp variant c.817_818GC>AA in SP6, the gene encoding the SP6 transcription factor, in a Caucasian family with autosomal dominant hypoplastic AI. The resulting missense protein change, p.(Ala273Lys), is predicted to alter a DNA-binding residue in the first of three zinc fingers. SP6 has been shown to be crucial to both proliferation of the IEE and to its differentiation into ameloblasts. SP6 has also been implicated as an AI candidate gene through its study in rodent models. We investigated the effect of the missense variant in SP6 (p.(Ala273Lys)) using surface plasmon resonance protein-DNA binding studies. We identified a potential SP6 binding motif in the AMBN proximal promoter sequence and showed that wild-type (WT) SP6 binds more strongly to it than the mutant protein. We hypothesize that SP6 variants may be a very rare cause of AI due to the critical roles of SP6 in development and that the relatively mild effect of the missense variant identified in this study is sufficient to affect amelogenesis causing AI, but not so severe as to be incompatible with life. We suggest that current AI cohorts, both with autosomal recessive and dominant disease, be screened for SP6 variants.
MeSH term(s)	Adaptor Proteins, Signal Transducing/genetics ; Ameloblasts/metabolism ; Ameloblasts/pathology ; Amelogenesis Imperfecta/genetics ; Amelogenesis Imperfecta/pathology ; Autophagy-Related Proteins/genetics ; Cell Differentiation/genetics ; Cell Proliferation/genetics ; DNA-Binding Proteins/genetics ; Dental Enamel/growth & development ; Dental Enamel/pathology ; Dental Enamel Proteins/genetics ; Female ; Genetic Predisposition to Disease ; Haplotypes ; Humans ; Kruppel-Like Transcription Factors/genetics ; Male ; Mutation, Missense/genetics ; Pedigree ; Promoter Regions, Genetic/genetics ; Tooth/growth & development ; Tooth/pathology ; Whole Exome Sequencing
Chemical Substances	Adaptor Proteins, Signal Transducing ; Autophagy-Related Proteins ; DNA-Binding Proteins ; Dental Enamel Proteins ; KLF14 protein, human ; Kruppel-Like Transcription Factors ; PLEKHM1 protein, human
Language	English
Publishing date	2020-03-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1108742-0
ISSN	1460-2083 ; 0964-6906
ISSN (online)	1460-2083
ISSN	0964-6906
DOI	10.1093/hmg/ddaa041
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Zs.A 3469: Show issues

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Article ; Online: Immune Profiling To Predict Outcome of Clostridioides difficile Infection.

Abhyankar, Mayuresh M / Ma, Jennie Z / Scully, Kenneth W / Nafziger, Andrew J / Frisbee, Alyse L / Saleh, Mahmoud M / Madden, Gregory R / Hays, Ann R / Poulter, Mendy / Petri, William A

mBio

2020 Volume 11, Issue 3

Abstract: There is a pressing need for biomarker-based models to predict mortality from and recurrence ... ...

Abstract	There is a pressing need for biomarker-based models to predict mortality from and recurrence of
MeSH term(s)	Aged ; Biomarkers/blood ; Clostridioides difficile/pathogenicity ; Clostridium Infections/diagnosis ; Clostridium Infections/immunology ; Clostridium Infections/mortality ; Cross Infection/diagnosis ; Cross Infection/immunology ; Cross Infection/microbiology ; Cytokines/blood ; Cytokines/immunology ; Female ; Humans ; Logistic Models ; Male ; Middle Aged ; Mortality ; Precision Medicine ; Proportional Hazards Models ; Recurrence ; Retrospective Studies ; Risk Factors
Chemical Substances	Biomarkers ; Cytokines
Language	English
Publishing date	2020-05-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.00905-20
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Article ; Online: Tropical forest responses to increasing atmospheric CO

Cernusak, Lucas A / Winter, Klaus / Dalling, James W / Holtum, Joseph A M / Jaramillo, Carlos / K Rner, Christian / Leakey, Andrew D B / Norby, Richard J / Poulter, Benjamin / Turner, Benjamin L / Wright, S Joseph

Functional plant biology : FPB

2020 Volume 40, Issue 6, Page(s) 531–551

Abstract: Elevated atmospheric CO2 concentrations (ca) will undoubtedly affect the metabolism of tropical forests worldwide; however, critical aspects of how tropical forests will respond remain largely unknown. Here, we review the current state of knowledge about ...

Abstract	Elevated atmospheric CO2 concentrations (ca) will undoubtedly affect the metabolism of tropical forests worldwide; however, critical aspects of how tropical forests will respond remain largely unknown. Here, we review the current state of knowledge about physiological and ecological responses, with the aim of providing a framework that can help to guide future experimental research. Modelling studies have indicated that elevated ca can potentially stimulate photosynthesis more in the tropics than at higher latitudes, because suppression of photorespiration by elevated ca increases with temperature. However, canopy leaves in tropical forests could also potentially reach a high temperature threshold under elevated ca that will moderate the rise in photosynthesis. Belowground responses, including fine root production, nutrient foraging and soil organic matter processing, will be especially important to the integrated ecosystem response to elevated ca. Water use efficiency will increase as ca rises, potentially impacting upon soil moisture status and nutrient availability. Recruitment may be differentially altered for some functional groups, potentially decreasing ecosystem carbon storage. Whole-forest CO2 enrichment experiments are urgently needed to test predictions of tropical forest functioning under elevated ca. Smaller scale experiments in the understorey and in gaps would also be informative, and could provide stepping stones towards stand-scale manipulations.
Language	English
Publishing date	2020-06-02
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2071582-1
ISSN	1445-4416 ; 1445-4408
ISSN (online)	1445-4416
ISSN	1445-4408
DOI	10.1071/FP12309
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