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  1. Article ; Online: Customized polymethylmethacrylate cranioplasty using a low-cost 3-dimensional printed mold

    Bogdanović Ivan / Milisavljević Filip / Miljković Aleksandar / Jovanović Nemanja / Ilić Rosanda

    Srpski Arhiv za Celokupno Lekarstvo, Vol 150, Iss 1-2, Pp 91-

    2022  Volume 95

    Abstract: Introduction. Significant cranial defects result from a decompressive craniectomy following head trauma, malignant brain edema, intracranial hemorrhage, or resection of tumor affected bone. Unrepaired cranial defects are not just a tremendous esthetic ... ...

    Abstract Introduction. Significant cranial defects result from a decompressive craniectomy following head trauma, malignant brain edema, intracranial hemorrhage, or resection of tumor affected bone. Unrepaired cranial defects are not just a tremendous esthetic problem. The underlying brain is unprotected, prone to injury, and this state can lead to the so-called “syndrome of the trephined” with mood instability, headaches, and even a neurological deficit. Currently, there is no widely accepted uniform technique of cranial vault shape restoration. Combining 3D technology with the use of polymethylmethacrylate is a challenging field that can bring good functional and aesthetic results and, in the case of smart design, become efficient, low-cost technology. We offer a possible solution to a problem that would be acceptable in neurosurgical practice. Case outline. We present a 37-year-old male patient with a massive hemicranial defect as a consequence of previous decompressive craniectomy following severe craniocerebral injury the previous year. Together with engineers from the appropriate 3D modeling studio, we have designed a two-part mold by laser printing technology using biocompatible advanced polyamide. We made a customized polymethylmethacrylate graft intraoperatively using this mold and achieved good aesthetic results. Conclusion. Reports of 3D printing assisted cranioplasties are growing, describing different techniques and cost- estimation. We hope to introduce a low-cost and simple method for repairing a skull defect.
    Keywords craniectomy ; cranioplasty ; skull defect ; polymethylmethacrylate ; 3d printing ; Medicine ; R
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Serbian Medical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Arterial supply and morphological characteristics of sympathetic neurons in the human superior cervical ganglion.

    Boljanović, Jelena / Milisavljević, Milan / Latas, Milan / Puškaš, Laslo / Bogosavljević, Nikola / Vujačić, Marko / Aleksandrić, Dejan / Ćetković, Dejan / Branković, Nemanja / Dožić, Aleksandra / Ćetković, Mila

    Frontiers in neuroanatomy

    2024  Volume 18, Page(s) 1372180

    Abstract: The aim of this study was the micromorphological analysis of the distribution of microvessels, mast cells and ganglionic neurons in two parts, proximal and distal of the human superior cervical sympathetic ganglions (SCSGs). Statistical analyses were ... ...

    Abstract The aim of this study was the micromorphological analysis of the distribution of microvessels, mast cells and ganglionic neurons in two parts, proximal and distal of the human superior cervical sympathetic ganglions (SCSGs). Statistical analyses were applied to detect the possible metric regional differences in their densities. Five injected human SCSGs with colored India ink and gelatin were microdissected and examined. Second group of five human SCSGs was prepared and serially sliced for CD34 and mast cell tryptase immunostaining. The microscopic fields of two parts of the SCSGs were analyzed for the following quantifications: microvessel density (MVD), mast cell density (MCD), and ganglionic cell count and measurements. The mean number of CD34-positive microvessels in microscopic fields, the MVD, had a value of 83 for the upper parts, and 82.7 for the lower parts of SCSGs. The mean number of tryptase-positive mast cells in microscopic fields, the MCD, was 4.5 in the proximal parts, and 4.7 in the distal parts of SCSGs. The mean number of ganglionic neurons in microscopic fields was 19.5 in the proximal parts, and 19.8 in the distal parts of SCSGs. The density of CD34-positive microvessels, the density of tryptase-positive mast cells, and the density, mean diameters and mean areas of ganglionic neurons were not significantly different in two observed parts, upper and lower of the SCSGs. In conclusion, the distributions of microvessels, mast cells, and neurons in two parts of the SCSGs were uniform with no specific micromorphological variations, there is a homogenous vascular and cellular pattern within the SCSGs.
    Language English
    Publishing date 2024-03-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2452969-2
    ISSN 1662-5129
    ISSN 1662-5129
    DOI 10.3389/fnana.2024.1372180
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Enzymatic synthesis of base-modified RNA by T7 RNA polymerase. A systematic study and comparison of 5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside triphosphates as substrates.

    Milisavljevič, Nemanja / Perlíková, Pavla / Pohl, Radek / Hocek, Michal

    Organic & biomolecular chemistry

    2018  Volume 16, Issue 32, Page(s) 5800–5807

    Abstract: We synthesized a small library of eighteen 5-substituted pyrimidine or 7-substituted 7-deazapurine nucleoside triphosphates bearing methyl, ethynyl, phenyl, benzofuryl or dibenzofuryl groups through cross-coupling reactions of nucleosides followed by ... ...

    Abstract We synthesized a small library of eighteen 5-substituted pyrimidine or 7-substituted 7-deazapurine nucleoside triphosphates bearing methyl, ethynyl, phenyl, benzofuryl or dibenzofuryl groups through cross-coupling reactions of nucleosides followed by triphosphorylation or through direct cross-coupling reactions of halogenated nucleoside triphosphates. We systematically studied the influence of the modification on the efficiency of T7 RNA polymerase catalyzed synthesis of modified RNA and found that modified ATP, UTP and CTP analogues bearing smaller modifications were good substrates and building blocks for the RNA synthesis even in difficult sequences incorporating multiple modified nucleotides. Bulky dibenzofuryl derivatives of ATP and GTP were not substrates for the RNA polymerase. In the case of modified GTP analogues, a modified procedure using a special promoter and GMP as initiator needed to be used to obtain efficient RNA synthesis. The T7 RNA polymerase synthesis of modified RNA can be very efficiently used for synthesis of modified RNA but the method has constraints in the sequence of the first three nucleotides of the transcript, which must contain a non-modified G in the +1 position.
    MeSH term(s) Adenosine Triphosphate/analogs & derivatives ; Adenosine Triphosphate/metabolism ; Bacteriophage T7/enzymology ; Cytidine Triphosphate/analogs & derivatives ; Cytidine Triphosphate/metabolism ; DNA-Directed RNA Polymerases/metabolism ; Purine Nucleosides/chemistry ; Purine Nucleosides/metabolism ; Purines/chemistry ; Purines/metabolism ; Pyrimidine Nucleosides/chemistry ; Pyrimidine Nucleosides/metabolism ; RNA/chemistry ; RNA/metabolism ; Substrate Specificity ; Uridine Triphosphate/analogs & derivatives ; Uridine Triphosphate/metabolism ; Viral Proteins/metabolism
    Chemical Substances 7-deazapurine ; Purine Nucleosides ; Purines ; Pyrimidine Nucleosides ; Viral Proteins ; RNA (63231-63-0) ; Cytidine Triphosphate (65-47-4) ; Adenosine Triphosphate (8L70Q75FXE) ; bacteriophage T7 RNA polymerase (EC 2.7.7.-) ; DNA-Directed RNA Polymerases (EC 2.7.7.6) ; Uridine Triphosphate (UT0S826Z60)
    Language English
    Publishing date 2018-10-02
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2097583-1
    ISSN 1477-0539 ; 1477-0520
    ISSN (online) 1477-0539
    ISSN 1477-0520
    DOI 10.1039/c8ob01498a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Design, Synthesis, and Biochemical and Biological Evaluation of Novel 7-Deazapurine Cyclic Dinucleotide Analogues as STING Receptor Agonists.

    Vavřina, Zdeněk / Perlíková, Pavla / Milisavljević, Nemanja / Chevrier, Florian / Smola, Miroslav / Smith, Joshua / Dejmek, Milan / Havlíček, Vojtěch / Buděšínský, Miloš / Liboska, Radek / Vaneková, Lenka / Brynda, Jiří / Boura, Evzen / Řezáčová, Pavlína / Hocek, Michal / Birkuš, Gabriel

    Journal of medicinal chemistry

    2022  Volume 65, Issue 20, Page(s) 14082–14103

    Abstract: Cyclic dinucleotides (CDNs) are second messengers that activate stimulator of interferon genes (STING). The cGAS-STING pathway plays a promising role in cancer immunotherapy. Here, we describe the synthesis of CDNs containing 7-substituted 7-deazapurine ... ...

    Abstract Cyclic dinucleotides (CDNs) are second messengers that activate stimulator of interferon genes (STING). The cGAS-STING pathway plays a promising role in cancer immunotherapy. Here, we describe the synthesis of CDNs containing 7-substituted 7-deazapurine moiety. We used mouse cyclic GMP-AMP synthase and bacterial dinucleotide synthases for the enzymatic synthesis of CDNs. Alternatively, 7-(het)aryl 7-deazapurine CDNs were prepared by Suzuki-Miyaura cross-couplings. New CDNs were tested in biochemical and cell-based assays for their affinity to human STING. Eight CDNs showed better activity than 2'3'-
    MeSH term(s) Mice ; Animals ; Humans ; Nucleotides, Cyclic/chemistry ; Ligands ; Membrane Proteins/metabolism ; Nucleotidyltransferases ; Cytokines ; Interferons
    Chemical Substances Nucleotides, Cyclic ; 7-deazapurine ; Ligands ; Membrane Proteins ; Nucleotidyltransferases (EC 2.7.7.-) ; Cytokines ; Interferons (9008-11-1)
    Language English
    Publishing date 2022-10-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218133-2
    ISSN 1520-4804 ; 0022-2623
    ISSN (online) 1520-4804
    ISSN 0022-2623
    DOI 10.1021/acs.jmedchem.2c01305
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Synthesis and Antitrypanosomal Activity of 6-Substituted 7-Methyl-7-deazapurine Nucleosides.

    Perlíková, Pavla / Krajczyk, Anna / Doleželová, Eva / Slapničková, Martina / Milisavljevic, Nemanja / Slavětínská, Lenka Poštová / Tloušt'ová, Eva / Gurská, Soňa / Džubák, Petr / Hajdúch, Marián / Zíková, Alena / Hocek, Michal

    ACS infectious diseases

    2021  Volume 7, Issue 4, Page(s) 917–926

    Abstract: Human African Trypanosomiasis caused ... ...

    Abstract Human African Trypanosomiasis caused by
    MeSH term(s) Antiprotozoal Agents ; Humans ; Nucleosides/pharmacology ; Purines ; Ribonucleosides
    Chemical Substances 7-deazapurine ; Antiprotozoal Agents ; Nucleosides ; Purines ; Ribonucleosides
    Language English
    Publishing date 2021-03-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2373-8227
    ISSN (online) 2373-8227
    DOI 10.1021/acsinfecdis.1c00062
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Oesophageal safety in voltage-guided atrial fibrillation ablation using ablation index or contact force only: a prospective comparison.

    Schade, Anja / Costello-Boerrigter, Lisa / Deneke, Thomas / Steinborn, Frank / Chapran, Mykhaylo / Vathie, Koroush / Milisavljevic, Nemanja / Franz, Marcus / Surber, Ralf / Assani, Mohamad / Hamo, Hussam / Khshfeh, Muhammed / Lauten, Alexander / Mattea, Violeta

    Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology

    2022  Volume 24, Issue 12, Page(s) 1909–1916

    Abstract: Aims: Left atrial ablation using radiofrequency (RF) is associated with endoscopically detected thermal oesophageal lesions (EDELs). The aim of this study was to compare EDEL occurrence after conventional contact force-guided (CFG) RF ablation vs. an ... ...

    Abstract Aims: Left atrial ablation using radiofrequency (RF) is associated with endoscopically detected thermal oesophageal lesions (EDELs). The aim of this study was to compare EDEL occurrence after conventional contact force-guided (CFG) RF ablation vs. an ablation index-guided (AIG) approach in clinical routine of voltage-guided ablation (VGA). Predictors of EDEL were also assessed.
    Methods and results: This study compared CFG (n = 100) with AIG (n = 100) in consecutive atrial fibrillation ablation procedures, in which both pulmonary vein isolation and VGA were performed. In the AIG group, AI targets were ≥500 anteriorly and ≥350-400 posteriorly. Upper endoscopy was performed after ablation.The CFG and AIG groups had comparable baseline characteristics. The EDEL occurred in 6 and 5% (P = 0.86) in the CFG and AIG groups, respectively. Category 2 lesions occurred in 4 and 2% (P = 0.68), respectively. All EDEL healed under proton pump inhibitor therapy. The AI > 520 was the only predictor of EDEL [odds ratio (OR) 3.84; P = 0.039]. The more extensive Category 2 lesions were predicted by: AI max > 520 during posterior ablation (OR 7.05; P = 0.042), application of posterior or roof lines (OR 5.19; P = 0.039), existence of cardiomyopathy (OR 4.93; P = 0.047), and CHA2DS2-VASc score (OR 1.71; P = 0.044). The only Category 2 lesion with AI max < 520 (467) occurred in a patient with low body mass index.
    Conclusions: Both methods were comparable with respect to clinical complications and EDEL. In consideration of previous reconnection data and our study results regarding oesophageal safety, optimal AI target range might be between 400 and 450.
    Language English
    Publishing date 2022-07-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 1449879-0
    ISSN 1532-2092 ; 1099-5129
    ISSN (online) 1532-2092
    ISSN 1099-5129
    DOI 10.1093/europace/euac103
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Antiviral Activity of 7-Substituted 7-Deazapurine Ribonucleosides, Monophosphate Prodrugs, and Triphoshates against Emerging RNA Viruses.

    Milisavljevic, Nemanja / Konkolová, Eva / Kozák, Jaroslav / Hodek, Jan / Veselovská, Lucia / Sýkorová, Veronika / Čížek, Karel / Pohl, Radek / Eyer, Luděk / Svoboda, Pavel / Růžek, Daniel / Weber, Jan / Nencka, Radim / Bouřa, Evžen / Hocek, Michal

    ACS infectious diseases

    2021  Volume 7, Issue 2, Page(s) 471–478

    Abstract: A series of 7-deazaadenine ribonucleosides bearing alkyl, alkenyl, alkynyl, aryl, or hetaryl groups at position 7 as well as their 5'- ...

    Abstract A series of 7-deazaadenine ribonucleosides bearing alkyl, alkenyl, alkynyl, aryl, or hetaryl groups at position 7 as well as their 5'-
    MeSH term(s) Antiviral Agents/pharmacology ; COVID-19/drug therapy ; COVID-19/virology ; Cell Line, Tumor ; Dengue Virus/drug effects ; Encephalitis Viruses, Tick-Borne/drug effects ; Humans ; Phosphates/pharmacology ; Prodrugs/pharmacology ; Purine Nucleosides ; Purines/pharmacology ; RNA Viruses/drug effects ; RNA-Dependent RNA Polymerase/metabolism ; Ribonucleosides/pharmacology ; SARS-CoV-2/drug effects ; West Nile virus/drug effects ; Zika Virus/drug effects
    Chemical Substances 7-deazapurine ; Antiviral Agents ; Phosphates ; Prodrugs ; Purine Nucleosides ; Purines ; Ribonucleosides ; RNA-Dependent RNA Polymerase (EC 2.7.7.48)
    Language English
    Publishing date 2021-01-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2373-8227
    ISSN (online) 2373-8227
    DOI 10.1021/acsinfecdis.0c00829
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Celiac disease diagnosed after uncomplicated pregnancy in a patient with history of bulimia nervosa.

    Milisavljević, Nemanja / Cvetković, Mirjana / Nikolić, Goran / Filipović, Branka / Milinić, Nikola

    Vojnosanitetski pregled

    2013  Volume 70, Issue 1, Page(s) 77–79

    Abstract: Introduction: The association between celiac disease and eating disorders has been very rarely reported. This is the first report on celiac disease associated with bulimia in this part of Europe.: Case report: An adult female patient with history of ... ...

    Abstract Introduction: The association between celiac disease and eating disorders has been very rarely reported. This is the first report on celiac disease associated with bulimia in this part of Europe.
    Case report: An adult female patient with history of bulimia and one uncomplicated pregnancy was admitted to the Gastroenterology Department, due to long lasting dyspeptic symptoms, constipation, major weight loss and fatigue. After positive serological screening, the diagnosis of celiac disease was confirmed with histopathology examination of duodenal biopsy specimen.
    Conclusion: Complicated interactions between celiac disease and bulimia can make them difficult to diagnose and treat. It is important to consider the presence of celiac disease in patients with bulimia and gastrointestinal symptoms.
    MeSH term(s) Adult ; Bulimia/complications ; Celiac Disease/complications ; Celiac Disease/diagnosis ; Female ; Humans ; Pregnancy ; Pregnancy Complications/diagnosis
    Language English
    Publishing date 2013-01-31
    Publishing country Serbia
    Document type Case Reports ; Journal Article
    ZDB-ID 123795-0
    ISSN 0042-8450
    ISSN 0042-8450
    DOI 10.2298/vsp1301077m
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Dilated cardiomyopathy associated with celiac disease: case report and literature review.

    Milisavljević, Nemanja / Cvetković, Mirjana / Nikolić, Goran / Filipović, Branka / Milinić, Nikola

    Srpski arhiv za celokupno lekarstvo

    2012  Volume 140, Issue 9-10, Page(s) 641–643

    Abstract: Introduction: Celiac disease is an inflammatory condition of the small intestinal mucosa induced by gluten consumption in genetically susceptible individuals, leading to a spectrum of gastrointestinal presentation. A number of autoimmune and other ... ...

    Abstract Introduction: Celiac disease is an inflammatory condition of the small intestinal mucosa induced by gluten consumption in genetically susceptible individuals, leading to a spectrum of gastrointestinal presentation. A number of autoimmune and other disorders are highly associated with celiac disease. Cardiomyopathy associated with celiac disease has been rarely reported in the literature.
    Case outline: We present a case of a 27-year-old male with one month history of diarrhea, weight loss, fatigue, dyspeptic symptoms, peripheral edema, and cardiac palpitations. After positive serological screening with immunoglobulin A anti-tissue transglutaminase antibody test, the diagnosis of celiac disease was confirmed with histopathology examination of duodenal biopsy specimen. Echocardiographic findings were consistent with acute myocarditis. After common causes of myocarditis had been excluded, probable celiac disease-associated autoimmune myocarditis was diagnosed. The patient was recommended to undergo a strict life-long gluten-free diet. IgA anti-transglutaminase antibodies, and anti-gliadin antibodies, were both significantly elevated during the 6-, 12- and 18-month follow-up. Low compliance to gluten-free diet in our patient led to progressive worsening of the left ventricular ejective fraction and other serious cardiac complications which warranted invasive cardiac interventions.
    Conclusion: Dilated cardiomyopathy associated with celiac disease is a serious condition which requires multidisciplinary approach involving gastroenterologist and cardiologist. Compliance with gluten-free diet is mandatory if patients are to avoid progression of cardiomyopathy. Screening of patients with idiopathic dilated cardiomyopathy for celiac disease is advisable.
    MeSH term(s) Adult ; Cardiomyopathy, Dilated/complications ; Cardiomyopathy, Dilated/diagnosis ; Cardiomyopathy, Dilated/therapy ; Celiac Disease/complications ; Celiac Disease/diagnosis ; Celiac Disease/therapy ; Humans ; Male
    Language English
    Publishing date 2012-09
    Publishing country Serbia
    Document type Case Reports ; Journal Article ; Review
    ZDB-ID 128567-1
    ISSN 0370-8179 ; 0354-2793 ; 0049-0210
    ISSN 0370-8179 ; 0354-2793 ; 0049-0210
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Celiac disease diagnosed after uncomplicated pregnancy in a patient with history of bulimia nervosa

    Milisavljević Nemanja / Cvetković Mirjana / Nikolić Goran / Filipović Branka / Milinić Nikola

    Vojnosanitetski Pregled, Vol 70, Iss 1, Pp 77-

    2013  Volume 79

    Abstract: Introduction. The association between celiac disease and eating disorders has been very rarely reported. This is the first report on celiac disease associated with bulimia in this part of Europe. Case report. An adult female patient with history of ... ...

    Abstract Introduction. The association between celiac disease and eating disorders has been very rarely reported. This is the first report on celiac disease associated with bulimia in this part of Europe. Case report. An adult female patient with history of bulimia and one uncomplicated pregnancy was admitted to the Gastroenterology Department, due to long lasting dyspeptic symptoms, constipation, major weight loss and fatigue. After positive serological screening, the diagnosis of celiac disease was confirmed with histopathology examination of duodenal biopsy specimen. Conclusion. Complicated interactions between celiac disease and bulimia can make them difficult to diagnose and treat. It is important to consider the presence of celiac disease in patients with bulimia and gastrointestinal symptoms.
    Keywords celiac disease ; eating disorders ; pregnancy ; diagnosis ; Medicine (General) ; R5-920
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Military Health Department, Ministry of Defance, Serbia
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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