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Article ; Online: Effect of liver resection-induced increases in hepatic venous pressure gradient on development of postoperative acute kidney injury.

Reiterer, Christian / Taschner, Alexander / Luf, Florian / Hecking, Manfred / Tamandl, Dietmar / Zotti, Oliver / Reiberger, Thomas / Starlinger, Patrick / Mandorfer, Mattias / Fleischmann, Edith

BMC nephrology

2022 Volume 23, Issue 1, Page(s) 21

Abstract: ... 12 of patients with increasing HVPG, but only in 2/9 of patients with stable ΔHVPG (p = 0.302 ... ΔHVPG. (p = 0.009). Biomarkers reflecting RAAS and SNS activity remained similar in patients ...

Abstract	Background: The impact of changes in portal pressure before and after liver resection (defined as ΔHVPG) on postoperative kidney function remains unknown. Therefore, we investigated the effect of ΔHVPG on (i) the incidence of postoperative AKI and (ii) the renin-angiotensin system (RAAS) and sympathetic nervous system (SNS) activity. Methods: We included 30 patients undergoing partial liver resection. Our primary outcome was postoperative AKI according to KDIGO criteria. For our secondary outcome we assessed the plasma renin, aldosterone, noradrenaline, adrenaline, dopamine and vasopressin concentrations prior and 2 h after induction of anaesthesia, on the first and fifth postoperative day. HVPG was measured prior and immediately after liver resection. Results: ΔHVPG could be measured in 21 patients with 12 patients HVPG showing increases in HVPG (∆HVPG≥1 mmHg) while 9 patients remained stable. AKI developed in 7/12 of patients with increasing HVPG, but only in 2/9 of patients with stable ΔHVPG (p = 0.302). Noradrenalin levels were significantly higher in patients with increasing ΔHVPG than in patients with stable ΔHVPG. (p = 0.009). Biomarkers reflecting RAAS and SNS activity remained similar in patients with increasing vs. stable ΔHVPG. Conclusions: Patients with increased HVPG had higher postoperative creatinine concentrations, however, the incidence of AKI was similar between patients with increased versus stable HVPG.
MeSH term(s)	Acute Kidney Injury/epidemiology ; Acute Kidney Injury/etiology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Female ; Hepatectomy/methods ; Humans ; Incidence ; Male ; Middle Aged ; Portal Pressure ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Renin-Angiotensin System/physiology ; Retrospective Studies ; Sympathetic Nervous System/physiopathology ; Young Adult
Language	English
Publishing date	2022-01-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2041348-8
ISSN	1471-2369 ; 1471-2369
ISSN (online)	1471-2369
ISSN	1471-2369
DOI	10.1186/s12882-021-02658-7
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Article ; Online: Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma.

Nature communications

2024 Volume 15, Issue 1, Page(s) 3422

Abstract: Targeting Anaplastic lymphoma kinase (ALK) is a promising therapeutic strategy for aberrant ALK-expressing malignancies including neuroblastoma, but resistance to ALK tyrosine kinase inhibitors (ALK TKI) is a distinct possibility necessitating drug ... ...

Abstract	Targeting Anaplastic lymphoma kinase (ALK) is a promising therapeutic strategy for aberrant ALK-expressing malignancies including neuroblastoma, but resistance to ALK tyrosine kinase inhibitors (ALK TKI) is a distinct possibility necessitating drug combination therapeutic approaches. Using high-throughput, genome-wide CRISPR-Cas9 knockout screens, we identify miR-1304-5p loss as a desensitizer to ALK TKIs in aberrant ALK-expressing neuroblastoma; inhibition of miR-1304-5p decreases, while mimics of this miRNA increase the sensitivity of neuroblastoma cells to ALK TKIs. We show that miR-1304-5p targets NRAS, decreasing cell viability via induction of apoptosis. It follows that the farnesyltransferase inhibitor (FTI) lonafarnib in addition to ALK TKIs act synergistically in neuroblastoma, inducing apoptosis in vitro. In particular, on combined treatment of neuroblastoma patient derived xenografts with an FTI and an ALK TKI complete regression of tumour growth is observed although tumours rapidly regrow on cessation of therapy. Overall, our data suggests that combined use of ALK TKIs and FTIs, constitutes a therapeutic approach to treat high risk neuroblastoma although prolonged therapy is likely required to prevent relapse.
MeSH term(s)	Neuroblastoma/drug therapy ; Neuroblastoma/genetics ; Neuroblastoma/pathology ; Neuroblastoma/metabolism ; Anaplastic Lymphoma Kinase/genetics ; Anaplastic Lymphoma Kinase/metabolism ; Anaplastic Lymphoma Kinase/antagonists & inhibitors ; Humans ; Animals ; Farnesyltranstransferase/antagonists & inhibitors ; Farnesyltranstransferase/metabolism ; Cell Line, Tumor ; Protein Kinase Inhibitors/pharmacology ; Protein Kinase Inhibitors/therapeutic use ; MicroRNAs/genetics ; MicroRNAs/metabolism ; GTP Phosphohydrolases/genetics ; GTP Phosphohydrolases/metabolism ; Pyridines/pharmacology ; Pyridines/therapeutic use ; Piperidines/pharmacology ; Piperidines/therapeutic use ; Mice ; Xenograft Model Antitumor Assays ; Membrane Proteins/metabolism ; Membrane Proteins/genetics ; Apoptosis/drug effects ; Apoptosis/genetics ; Drug Resistance, Neoplasm/genetics ; Drug Resistance, Neoplasm/drug effects ; Mutation ; Gene Expression Regulation, Neoplastic/drug effects ; Female ; Drug Synergism ; Dibenzocycloheptenes
Chemical Substances	Anaplastic Lymphoma Kinase (EC 2.7.10.1) ; Farnesyltranstransferase (EC 2.5.1.29) ; ALK protein, human (EC 2.7.10.1) ; Protein Kinase Inhibitors ; MicroRNAs ; lonafarnib (IOW153004F) ; GTP Phosphohydrolases (EC 3.6.1.-) ; Pyridines ; Piperidines ; NRAS protein, human (EC 3.6.1.-) ; Membrane Proteins ; Dibenzocycloheptenes
Language	English
Publishing date	2024-04-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-47771-x
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Article: Refined high-content imaging-based phenotypic drug screening in zebrafish xenografts.

Sturtzel, C / Grissenberger, S / Bozatzi, P / Scheuringer, E / Wenninger-Weinzierl, A / Zajec, Z / Dernovšek, J / Pascoal, S / Gehl, V / Kutsch, A / Granig, A / Rifatbegovic, F / Carre, M / Lang, A / Valtingojer, I / Moll, J / Lötsch, D / Erhart, F / Widhalm, G /

Surdez, D / Delattre, O / André, N / Stampfl, J / Tomašič, T / Taschner-Mandl, S / Distel, M

NPJ precision oncology

2023 Volume 7, Issue 1, Page(s) 44

Abstract: Zebrafish xenotransplantation models are increasingly applied for phenotypic drug screening to identify small compounds for precision oncology. Larval zebrafish xenografts offer the opportunity to perform drug screens at high-throughput in a complex in ... ...

Abstract	Zebrafish xenotransplantation models are increasingly applied for phenotypic drug screening to identify small compounds for precision oncology. Larval zebrafish xenografts offer the opportunity to perform drug screens at high-throughput in a complex in vivo environment. However, the full potential of the larval zebrafish xenograft model has not yet been realized and several steps of the drug screening workflow still await automation to increase throughput. Here, we present a robust workflow for drug screening in zebrafish xenografts using high-content imaging. We established embedding methods for high-content imaging of xenografts in 96-well format over consecutive days. In addition, we provide strategies for automated imaging and analysis of zebrafish xenografts including automated tumor cell detection and tumor size analysis over time. We also compared commonly used injection sites and cell labeling dyes and show specific site requirements for tumor cells from different entities. We demonstrate that our setup allows us to investigate proliferation and response to small compounds in several zebrafish xenografts ranging from pediatric sarcomas and neuroblastoma to glioblastoma and leukemia. This fast and cost-efficient assay enables the quantification of anti-tumor efficacy of small compounds in large cohorts of a vertebrate model system in vivo. Our assay may aid in prioritizing compounds or compound combinations for further preclinical and clinical investigations.
Language	English
Publishing date	2023-05-18
Publishing country	England
Document type	Journal Article ; Review
ISSN	2397-768X
ISSN	2397-768X
DOI	10.1038/s41698-023-00386-9
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Article ; Online: Two-Dimensional Laser Melting of Lunar Regolith Simulant Using the MOONRISE Payload on a Mobile Manipulator.

Linke, Stefan / Voß, Anna / Ernst, Mathias / Taschner, Patrick A / Baasch, Julian / Stapperfend, Simon / Gerdes, Niklas / Koch, Jürgen / Weßels, Peter / Neumann, Jörg / Overmeyer, Ludger / Stoll, Enrico

3D printing and additive manufacturing

2022 Volume 9, Issue 3, Page(s) 223–231

Abstract: Laser melting experiments were carried out with the MOONRISE payload, installed on the mobile manipulator, MIRA3D. The MOONRISE payload was developed to demonstrate the feasibility of additive processing of lunar regolith with the help of lasers on the ... ...

Abstract	Laser melting experiments were carried out with the MOONRISE payload, installed on the mobile manipulator, MIRA3D. The MOONRISE payload was developed to demonstrate the feasibility of additive processing of lunar regolith with the help of lasers on the Moon within a lunar surface mission in the next years. The development of hardware for the flight to the moon is well advanced and, if successful, would pave the way for the use of laser melting for production of components from regolith. The aim of the experiments described in this article was to test the planned scenario on the Moon, especially the interaction between laser payload, manipulator, and soil surface, and to identify suitable process parameters for production of two-dimensional (2D) objects. The ability to produce 2D objects is an important intermediate step on the way to produce large three-dimensional structures such as habitats, walls, or foundations. During the experiments, specimens with a size of ∼20 × 20 × 4 mm were repeatedly produced. As analog material, two synthetic lunar soils produced with the modular regolith simulant systems from Technische Universität Braunschweig (TUBS) were used. The experiments were conducted under Earth gravity and atmospheric conditions. This article describes the hardware used, procedure for carrying out the experiments, and properties of the produced samples.
Language	English
Publishing date	2022-06-09
Publishing country	United States
Document type	Journal Article
ISSN	2329-7670
ISSN (online)	2329-7670
DOI	10.1089/3dp.2020.0323
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Article ; Online: The effect of supplemental oxygen on perioperative brain natriuretic peptide concentration in cardiac risk patients - a protocol for a prosprective randomized clinical trial.

Reiterer, Christian / Kabon, Barbara / von Sonnenburg, Markus Falkner / Starlinger, Patrick / Taschner, Alexander / Zotti, Oliver / Goshin, Julius / Drlicek, Gregor / Fleischmann, Edith

Trials

2020 Volume 21, Issue 1, Page(s) 400

Abstract: Background: Elevated postoperative N-terminal pro-B-type natriuretic peptide (NT-proBNP) concentrations are predictive for cardiac adverse events in noncardiac surgery. Studies indicate that supplemental oxygen decreases sympathetic nerve activity and ... ...

Abstract	Background: Elevated postoperative N-terminal pro-B-type natriuretic peptide (NT-proBNP) concentrations are predictive for cardiac adverse events in noncardiac surgery. Studies indicate that supplemental oxygen decreases sympathetic nerve activity and might, therefore, improve cardiovascular function. Thus, we will test the effect of perioperative supplemental oxygen administration on NT-proBNP release after surgery. Methods/design: We will conduct a single-center, double-blinded, randomized trial at the Medical University of Vienna, including 260 patients with increased cardiac risk factors undergoing moderate- to high-risk noncardiac surgery. Patients will be randomly assigned to receive 80% versus 30% oxygen during surgery and for 2 h postoperatively. The primary outcome will be the difference in maximum NT-proBNP release after surgery. As secondary outcomes we will assess the effect of supplemental oxygen on postoperative maximum troponin T concentration, oxidation-reduction potential, von Willebrand factor concentration and perioperative fluid requirements. We will perform outcome measurements 2 h after surgery, on postoperative day 1 and on postoperative day 3. The NT-proBNP concentration and the oxidation-reduction potential will also be measured within 72 h before discharge. Discussion: Our trial should determine whether perioperative supplemental oxygen administration will reduce the postoperative release of NT-proBNP in patients with preoperative increased cardiovascular risk factors undergoing noncardiac surgery. Trial registration: ClinicalTrials.gov, ID: NCT03366857. Registered on 8th December 2017.
MeSH term(s)	Abdomen/surgery ; Aged ; Austria/epidemiology ; Double-Blind Method ; Female ; Fluid Therapy/methods ; Fluid Therapy/statistics & numerical data ; Heart Diseases/complications ; Heart Diseases/physiopathology ; Heart Function Tests/methods ; Humans ; Male ; Middle Aged ; Natriuretic Peptide, Brain/blood ; Natriuretic Peptide, Brain/metabolism ; Oxidation-Reduction ; Oxygen/adverse effects ; Oxygen/supply & distribution ; Peptide Fragments/blood ; Perioperative Period ; Postoperative Period ; Predictive Value of Tests ; Prospective Studies ; Risk Assessment ; Risk Factors ; Sympathetic Nervous System/metabolism ; Sympathetic Nervous System/physiopathology ; Troponin T/metabolism ; von Willebrand Factor/metabolism
Chemical Substances	Peptide Fragments ; Troponin T ; pro-brain natriuretic peptide (1-76) ; von Willebrand Factor ; Natriuretic Peptide, Brain (114471-18-0) ; Oxygen (S88TT14065)
Language	English
Publishing date	2020-05-12
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	2040523-6
ISSN	1745-6215 ; 1468-6694 ; 1745-6215
ISSN (online)	1745-6215
ISSN	1468-6694 ; 1745-6215
DOI	10.1186/s13063-020-04336-9
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Article ; Online: Perioperative supplemental oxygen and oxidative stress in patients undergoing moderate- to high-risk major abdominal surgery - A subanalysis of randomized clinical trial.

Reiterer, Christian / Fleischmann, Edith / Taschner, Alexander / Adamowitsch, Nikolas / von Sonnenburg, Markus Falkner / Graf, Alexandra / Fraunschiel, Melanie / Starlinger, Patrick / Goschin, Julius / Kabon, Barbara

Journal of clinical anesthesia

2021 Volume 77, Page(s) 110614

Abstract: ... 162 mV,95% CI: -2.584 to 0.260; p = 0.109). On average, we observed a change in sORP of 5.288 mV (95 ... CI:4.633 to 5.913, p < 0.001) per day. cORP values did not differ significantly between the 80% and ... 30% oxygen group (effect estimate: -0.015μC, (95%CI: -0.062 to 0.032; p = 0.524). On average ...

Abstract	Study objective: Oxidative stress plays a pivotal role in the development and aggravation of cardiovascular diseases. The influence of intraoperative inspired oxygen concentrations on oxidative stress is still not entirely known. Therefore, we evaluated in this sub-study if supplemental oxygen affects the oxidation-reduction potential in patients at-risk for cardiovascular complications undergoing moderate- to high-risk major abdominal surgery. Design: Sub-study of a prospective parallel-arm double-blinded single-center superiority randomized trial. Setting: Operating room and postoperative recovery area. Intervention: Administration of 0.8 FiO Measurements: The primary outcome was the static oxidation-reduction potential (sORP) and the oxidation-reduction potential capacity (cORP) between both groups. The secondary outcome was the trend of sORP and cORP in the overall study population. We assessed sORP and cORP before induction of anesthesia, 2 h after induction of anesthesia, within 2 h after surgery and on the first and third postoperative day. Main results: 258 patients were analyzed. 128 patients were randomly assigned to the 80% oxygen group and 130 patients were randomly assigned to the 30% oxygen group. Postoperative sORP values did not differ significantly between the 80% and 30% oxygen group (effect estimate: -1.162 mV,95% CI: -2.584 to 0.260; p = 0.109). On average, we observed a change in sORP of 5.288 mV (95% CI:4.633 to 5.913, p < 0.001) per day. cORP values did not differ significantly between the 80% and 30% oxygen group (effect estimate: -0.015μC, (95%CI: -0.062 to 0.032; p = 0.524). On average, we observed a change in cORP values of -0.170μC (95%CI: -0.194 to -0.147, p < 0.001) per day. Conclusion: In contrast to previous reports, we could not find any evidence of an association between intraoperative supplemental oxygen and perioperative oxidative stress assessed by sORP and cORP. Trial registration: clinicaltrials.gov: NCT03366857https://clinicaltrials.gov/ct2/show/NCT03366857?term=vienna&cond=oxygen&draw=2&rank=1.
MeSH term(s)	Abdomen/surgery ; Humans ; Oxidative Stress ; Oxygen ; Postoperative Period ; Prospective Studies
Chemical Substances	Oxygen (S88TT14065)
Language	English
Publishing date	2021-11-29
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	1011618-7
ISSN	1873-4529 ; 0952-8180
ISSN (online)	1873-4529
ISSN	0952-8180
DOI	10.1016/j.jclinane.2021.110614
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Article ; Online: Corrigendum to "Perioperative supplemental oxygen and NT-proBNP concentrations after major abdominal surgery - A prospective randomized clinical trial" [Journal of Clinical Anaesthesia volume 73 (2021) article 110379].

Reiterer, Christian / Kabon, Barbara / Taschner, Alexander / von Sonnenburg, Markus Falkner / Graf, Alexandra / Adamowitsch, Nikolas / Starlinger, Patrick / Goschin, Julius / Fraunschiel, Melanie / Fleischmann, Edith

Journal of clinical anesthesia

2021 Volume 76, Page(s) 110579

Language	English
Publishing date	2021-11-10
Publishing country	United States
Document type	Published Erratum
ZDB-ID	1011618-7
ISSN	1873-4529 ; 0952-8180
ISSN (online)	1873-4529
ISSN	0952-8180
DOI	10.1016/j.jclinane.2021.110579
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Article ; Online: A Combination of Structural, Genetic, Phenotypic and Enzymatic Analyses Reveals the Importance of a Predicted Fucosyltransferase to Protein

Tomek, Markus B / Janesch, Bettina / Braun, Matthias L / Taschner, Manfred / Figl, Rudolf / Grünwald-Gruber, Clemens / Coyne, Michael J / Blaukopf, Markus / Altmann, Friedrich / Kosma, Paul / Kählig, Hanspeter / Comstock, Laurie E / Schäffer, Christina

Biomolecules

2021 Volume 11, Issue 12

Abstract: Diverse members of the Bacteroidetes phylum have general ... ...

Abstract	Diverse members of the Bacteroidetes phylum have general protein
MeSH term(s)	Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacteroides/enzymology ; Bacteroides/growth & development ; Bacteroides fragilis/enzymology ; Bacteroides fragilis/growth & development ; Carbohydrate Conformation ; Evolution, Molecular ; Fucosyltransferases/chemistry ; Fucosyltransferases/genetics ; Fucosyltransferases/metabolism ; Gene Expression Regulation, Bacterial ; Glycosylation ; Models, Molecular ; Pedobacter/enzymology ; Pedobacter/growth & development ; Polysaccharides/chemistry ; Polysaccharides/metabolism ; Tannerella forsythia/enzymology ; Tannerella forsythia/growth & development
Chemical Substances	Bacterial Proteins ; Polysaccharides ; Fucosyltransferases (EC 2.4.1.-)
Language	English
Publishing date	2021-11-30
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom11121795
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Article ; Online: Perioperative supplemental oxygen and NT-proBNP concentrations after major abdominal surgery - A prospective randomized clinical trial.

Reiterer, Christian / Kabon, Barbara / Taschner, Alexander / Falkner von Sonnenburg, Markus / Graf, Alexandra / Adamowitsch, Nikolas / Starlinger, Patrick / Goshin, Julius / Fraunschiel, Melanie / Fleischmann, Edith

Journal of clinical anesthesia

2021 Volume 73, Page(s) 110379

Abstract: Study objective: Supplemental oxygen is a simple method to improve arterial oxygen saturation and might therefore improve myocardial oxygenation. Thus, we tested whether intraoperative supplemental oxygen reduces the risk of impaired cardiac function ... ...

Abstract	Study objective: Supplemental oxygen is a simple method to improve arterial oxygen saturation and might therefore improve myocardial oxygenation. Thus, we tested whether intraoperative supplemental oxygen reduces the risk of impaired cardiac function diagnosed with NT-proBNP and myocardial injury after noncardiac surgery (MINS) diagnosed with high-sensitivity Troponin T. Design: Parallel-arm double-blinded single-centre superiority randomized trial. Setting: Operating room and postoperative recovery area. Patients: 260 patients over the age of 45 years at-risk for cardiovascular complications undergoing major abdominal surgery. Intervention: Administration of 80% versus 30% oxygen throughout surgery and for the first two postoperative hours. Measurements: The primary outcome was the postoperative maximum NT-proBNP concentration in both groups, which was assessed within 2 h after surgery, and on the first and third postoperative day. The secondary outcome was the incidence of MINS in both groups. Main results: 128 patients received 80% oxygen and 130 received 30% oxygen throughout surgery and for the first two postoperative hours. There was no significant difference in the median postoperative maximum NT-proBNP concentration between the 80% and the 30% oxygen group (989 pg.mL Conclusions: There was no beneficial effect of perioperative supplemental oxygen administration on postoperative NT-proBNP concentration and MINS. It seems likely that supplemental oxygen has no effect on the release of NT-proBNP in patients at-risk for cardiovascular complications undergoing major abdominal surgery. Trial registration: ClinicalTrials.gov: NCT03366857. https://clinicaltrials.gov/ct2/results?cond=NCT+03366857&term=&cntry=&state=&city=&dist=.
MeSH term(s)	Biomarkers ; Humans ; Middle Aged ; Natriuretic Peptide, Brain ; Oxygen ; Peptide Fragments ; Prospective Studies ; Risk Assessment
Chemical Substances	Biomarkers ; Peptide Fragments ; pro-brain natriuretic peptide (1-76) ; Natriuretic Peptide, Brain (114471-18-0) ; Oxygen (S88TT14065)
Language	English
Publishing date	2021-06-01
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	1011618-7
ISSN	1873-4529 ; 0952-8180
ISSN (online)	1873-4529
ISSN	0952-8180
DOI	10.1016/j.jclinane.2021.110379
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Article ; Online: The effect of the rpoSam allele on gene expression and stress resistance in Escherichia coli.

Galbiati, Heloisa F / Taschner, Natalia P / Spira, Beny

Archives of microbiology

2014 Volume 196, Issue 8, Page(s) 589–600

Abstract: ... found in E. coli strains. The rpoSam mutant expresses a truncated and partially functional RpoS protein ... Here, we present new evidence regarding rpoS polymorphism in common laboratory E. coli strains. One out ...

Abstract	The RNA polymerase associated with RpoS transcribes many genes related to stationary phase and stress survival in Escherichia coli. The DNA sequence of rpoS exhibits a high degree of polymorphism. A C to T transition at position 99 of the rpoS ORF, which results in a premature amber stop codon often found in E. coli strains. The rpoSam mutant expresses a truncated and partially functional RpoS protein. Here, we present new evidence regarding rpoS polymorphism in common laboratory E. coli strains. One out of the six tested strains carries the rpoSam allele, but expressed a full-length RpoS protein owing to the presence of an amber supressor mutation. The rpoSam allele was transferred to a non-suppressor background and tested for RpoS level, stress resistance and for the expression of RpoS and sigma70-dependent genes. Overall, the rpoSam strain displayed an intermediate phenotype regarding stress resistance and the expression of σ(S)-dependent genes when compared to the wild-type rpoS(+) strain and to the rpoS null mutant. Surprisingly, overexpression of rpoSam had a differential effect on the expression of the σ(70)-dependent genes phoA and lacZ that, respectively, encode the enzymes alkaline phosphatase and β-galactosidase. The former was enhanced while the latter was inhibited by high levels of RpoSam.
MeSH term(s)	Alkaline Phosphatase/metabolism ; Alleles ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Base Sequence ; Codon, Nonsense ; Escherichia coli K12/genetics ; Escherichia coli K12/physiology ; Gene Expression ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Microbial Viability ; Phenotype ; Salt-Tolerance ; Sigma Factor/genetics ; Sigma Factor/metabolism ; Stress, Physiological ; beta-Galactosidase/genetics ; beta-Galactosidase/metabolism
Chemical Substances	Bacterial Proteins ; Codon, Nonsense ; Sigma Factor ; sigma factor KatF protein, Bacteria ; Alkaline Phosphatase (EC 3.1.3.1) ; beta-Galactosidase (EC 3.2.1.23)
Language	English
Publishing date	2014-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-014-0994-y
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