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Book: Mitigation of antimicrobial resistance / Vol 2

Panwar, Harsh / Sharma, Chetan / Lichtfouse, Eric

(Sustainable agriculture reviews ; 49)

2021

Author's details	Harsh Panwar, Chetan Sharma, Eric Lichtfouse, editors
Series title	Sustainable agriculture reviews ; 49 Mitigation of antimicrobial resistance
Collection	Mitigation of antimicrobial resistance
Language	English
Size	xv, 236 Seiten, Illustrationen, Diagramme
Publishing country	Switzerland
Document type	Book
HBZ-ID	HT020799149
ISBN	978-3-030-58258-6 ; 9783030582593 ; 3-030-58258-2 ; 3030582590
Database	Catalogue ZB MED Nutrition, Environment, Agriculture

In stock of ZB MED Bonn / Germany

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218/16	available for loan (reading room)	Freihandmagazin (U1)

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Book ; Collection: Mitigation of antimicrobial resistance

Panwar, Harsh / Sharma, Chetan / Lichtfouse, Eric

(Sustainable agriculture reviews)

2020

Author's details	Harsh Panwar, Chetan Sharma, Eric Lichtfouse, editors
Series title	Sustainable agriculture reviews
Language	English
Dates of publication	2020-9999
Publisher	Springer
Publishing place	Cham
Publishing country	Switzerland
Document type	Book ; Collection (display volumes)
HBZ-ID	HT020625439
Database	Catalogue ZB MED Nutrition, Environment, Agriculture

In stock of ZB MED Bonn / Germany

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Book: Mitigation of antimicrobial resistance / Vol 1

Panwar, Harsh / Sharma, Chetan / Lichtfouse, Eric

(Sustainable agriculture reviews ; 46)

2020

Author's details	Harsh Panwar, Chetan Sharma, Eric Lichtfouse, editors
Series title	Sustainable agriculture reviews ; 46 Mitigation of antimicrobial resistance
Collection	Mitigation of antimicrobial resistance
Language	English
Size	xv, 231 Seiten, Illustrationen, Diagramme
Publishing country	Switzerland
Document type	Book
HBZ-ID	HT020625443
ISBN	978-3-030-53023-5 ; 9783030530242 ; 3-030-53023-X ; 3030530248
Database	Catalogue ZB MED Nutrition, Environment, Agriculture

In stock of ZB MED Bonn / Germany

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208/289	available for loan (reading room)	Freihandmagazin (U1)

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Book ; Online: The NPC AI of The Last of Us

Panwar, Harsh

A case study

2022

Abstract: The Last of Us is a game focused on stealth, companionship and strategy. The game is based in a lonely world after the pandemic and thus it needs AI companions to gain the interest of players. There are three main NPCs the game has - Infected, Human ... ...

Abstract	The Last of Us is a game focused on stealth, companionship and strategy. The game is based in a lonely world after the pandemic and thus it needs AI companions to gain the interest of players. There are three main NPCs the game has - Infected, Human enemy and Buddy AIs. This case study talks about the challenges in front of the developers to create AI for these NPCs and the AI techniques they used to solve them. It also compares the challenges and approach with similar industry-leading games.
Keywords	Computer Science - Artificial Intelligence
Publishing date	2022-07-01
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Duplex real-time PCR methods for molecular detection and characterization of canine tick-borne haemoparasites from Punjab state, India.

Thomas, Aparna M / Singh, Harkirat / Panwar, Harsh / Sethi, Ram S / Singh, Nirbhay K

Molecular biology reports

2022 Volume 49, Issue 6, Page(s) 4451–4459

Abstract: Background: Microscopy is a routinely used technique for the diagnosis of canine tick-borne haemoparasitic diseases in various clinical laboratories worldwide. In an attempt to provide better diagnostic assay to the clients for effective management of ... ...

Abstract	Background: Microscopy is a routinely used technique for the diagnosis of canine tick-borne haemoparasitic diseases in various clinical laboratories worldwide. In an attempt to provide better diagnostic assay to the clients for effective management of these diseases duplex real-time PCR assays were applied. Methods and results: Blood samples (n = 338) aseptically collected from suspected dogs of Central Plain Zone of Punjab state, India were subjected to SYBR Green based real-time duplex PCR assays for simultaneous detection of B. vogeli & E. canis and B. gibsoni & H. canis. Results revealed an overall prevalence rate of canine tick-borne haemoparasites as 54.1%, amongst which H. canis was the predominant (25.4%), followed by B. gibsoni (16.3%), E. canis (10.7%) and B. vogeli (1.8%). Sensitivity and specificity of the duplex assays ranged from 59.04 to 100.0% and 58.12 to 92.52%, respectively and their strength of agreement was ″fair″ with kappa value statistics. A significant (p < 0.05) association between prevalence of B. gibsoni, H. canis and E. canis infection with risk factors like sex, breed, season and location was recorded. The ancestral background of the field isolates of haemoparasites was also studied by phylogenetic analysis of their nucleotide sequences. Conclusions: SYBR Green dye based duplex real-time PCR assays proved to be highly sensitive, specific, rapid and affordable diagnostic tests for use by clinicians to save the life of pets.
MeSH term(s)	Animals ; Babesia/genetics ; Dog Diseases/diagnosis ; Dog Diseases/epidemiology ; Dog Diseases/genetics ; Dogs ; Humans ; Phylogeny ; Real-Time Polymerase Chain Reaction ; Tick-Borne Diseases/epidemiology ; Tick-Borne Diseases/veterinary ; Ticks/parasitology
Language	English
Publishing date	2022-03-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-022-07286-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Development and validation of multiplex SYBR Green real-time PCR assays for detection and molecular surveillance of four tick-borne canine haemoparasites.

Padmaja, M / Singh, Harkirat / Panwar, Harsh / Jyoti / Singh, Niraj Kumar / Singh, Nirbhay Kumar

Ticks and tick-borne diseases

2022 Volume 13, Issue 3, Page(s) 101937

Abstract: Two multiplex SYBR Green based real-time PCR assays were standardized and evaluated to detect DNA from four canine haemoparasites (Babesia gibsoni, Babesia vogeli, Ehrlichia canis and Hepatozoon canis), along with internal controls from dogs from ... ...

Abstract	Two multiplex SYBR Green based real-time PCR assays were standardized and evaluated to detect DNA from four canine haemoparasites (Babesia gibsoni, Babesia vogeli, Ehrlichia canis and Hepatozoon canis), along with internal controls from dogs from selected districts of Punjab state, India. Amplicons of 126 bp, 337 bp, 234 bp and 106 bp corresponding to B. gibsoni (18S rRNA gene), B. vogeli (18S rRNA gene), E. canis (virB9 gene), and H. canis (18S rRNA gene) were obtained, without any non-specific amplification. Microscopic evaluation of 200 blood samples from dogs revealed the prevalence of B. gibsoni, E. canis and H. canis as 1.5%, 1.5% and 1.0%, respectively, while with the multiplex real-time PCR assays the values for B. gibsoni, B. vogeli, E. canis, and H. canis were 8.0%, 1.5%, 3.5% and 23.5%, respectively, with concurrent infections of B. gibsoni and H. canis (3.5%); E. canis and H. canis (2.0%) and B. gibsoni, B. vogeli, E. canis, and H. canis (0.5%). The diagnostic sensitivity of the multiplex real-time PCR assays with respect to microscopy in the detection of B. gibsoni, E. canis and H. canis was 100% while the specificity for B. gibsoni, B. vogeli, E. canis, and H. canis was 93%, 100%, 98% and 77%, respectively, revealing the respective strength of agreement as ″fair″, ″slight″, ″moderate″ and ″slight″ by kappa value statistics, and the data were statistically significant, for detection of B. gibsoni and E. canis infections, by Fisher's exact test. The analytical sensitivity of the multiplex PCR assays in detection of DNAs was 8.59 × 10
MeSH term(s)	Animals ; Benzothiazoles ; Diamines ; Dog Diseases/diagnosis ; Dog Diseases/epidemiology ; Dog Diseases/parasitology ; Dogs ; Multiplex Polymerase Chain Reaction/methods ; Multiplex Polymerase Chain Reaction/veterinary ; Quinolines ; Real-Time Polymerase Chain Reaction/veterinary ; Ticks
Chemical Substances	Benzothiazoles ; Diamines ; Quinolines ; SYBR Green I (163795-75-3)
Language	English
Publishing date	2022-03-08
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2541872-5
ISSN	1877-9603 ; 1877-959X
ISSN (online)	1877-9603
ISSN	1877-959X
DOI	10.1016/j.ttbdis.2022.101937
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria.

Duche, Rachael T / Singh, Anamika / Wandhare, Arundhati Ganesh / Sangwan, Vikas / Sihag, Manvesh Kumar / Nwagu, Tochukwu N T / Panwar, Harsh / Ezeogu, Lewis I

BMC microbiology

2023 Volume 23, Issue 1, Page(s) 142

Abstract: Introduction: Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission ... ...

Abstract	Introduction: Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission via functional foods is increasingly being emphasized. Objectives: This study screened potential probiotic lactic acid bacteria (LAB) strains for their phenotypic and genotypic antibiotic resistance profiles. Methods: Susceptibility to different antibiotics was assayed by the Kirby Bauer standard disc diffusion protocol. Both conventional and SYBR-RTq-PCR were used for detection of resistance coding genes. Results: A variable susceptibility pattern was documented against different antibiotic classes. LAB strains irrespective of origin displayed marked phenotypic resistance against cephalosporins, aminoglycosides, quinolones, glycopeptides; and methicillin among beta-lactams with few exceptions. In contrast, high sensitivity was recorded against macrolides, sulphonamides and carbapenems sub-group of beta-lactams with some variations. parC, associated with ciprofloxacin resistance was detected in 76.5% of the strains. Other prevalent resistant determinants observed were aac(6?)Ii (42.1%), ermB, ermC (29.4%), and tetM (20.5%). Six (?17.6%) of the isolates were free from genetic resistance determinants screened in this study. Conclusion: Study revealed presence of antibiotic resistance determinants among lactobacilli from both fermented foods and human sources.
MeSH term(s)	Humans ; Lactobacillales/genetics ; Nigeria ; Drug Resistance, Bacterial/genetics ; Microbial Sensitivity Tests ; Anti-Bacterial Agents/pharmacology ; Lactobacillus ; Fermented Foods ; beta-Lactams ; Probiotics
Chemical Substances	Anti-Bacterial Agents ; beta-Lactams
Language	English
Publishing date	2023-05-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2041505-9
ISSN	1471-2180 ; 1471-2180
ISSN (online)	1471-2180
ISSN	1471-2180
DOI	10.1186/s12866-023-02883-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus

Vishu Chaudhary / Priya Katyal / Harsh Panwar / Anil Kumar Puniya / Anuj Kumar Poonia

Environment Conservation Journal, Vol 23, Iss 1&

2022 Volume 2

Abstract: In the present study, Monascus red biopigment produced by solid-state fermentation was evaluated for its anti-microbial and antioxidative potential. The antibacterial activity through Scanning Electron Microscopy against Bacillus cereus, Escherichia coli, ...

Abstract	In the present study, Monascus red biopigment produced by solid-state fermentation was evaluated for its anti-microbial and antioxidative potential. The antibacterial activity through Scanning Electron Microscopy against Bacillus cereus, Escherichia coli, and Staphylococcus aureus was found to show morphological damage in some cells, as evidenced by the outflow of cell contents, deep craters, burst cells, and cell death at concentration of 200 µg/ml of biopigment. Moreover, antibacterial activity through agar well diffusion method against Bacillus cereus, Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa was in range of 2-6 mm by varying concentration of biopigment from 1 to 20 mg/ml.Next, the antifungal activity of the extracted biopigment was in the range of 2-9 mm for Aspergillus flavus, Fusarium oxysporum and Alternaria alternata. In addition, antioxidant efficacy of red biopigment through DPPH, ABTS and FRAP assay results was found to show 59.69 %, 91.1 %, and 15.22 % free radical scavenging activity. The results of this study revealed that red biopigment has potential to modulate the antimicrobial and antioxidative activity.
Keywords	Antimicrobial ; Antioxidative ; Food colorants ; Monascus purpureus ; Red Biopigment ; Environmental sciences ; GE1-350
Subject code	571
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	Action for Sustainable Efficacious Development and Awareness
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Duplex real-time PCR methods for molecular detection and characterization of canine tick-borne haemoparasites from Punjab state, India

Thomas, Aparna M. / Singh, Harkirat / Panwar, Harsh / Sethi, Ram S. / Singh, Nirbhay K.

Molecular biology reports. 2022 June, v. 49, no. 6

2022

Abstract: BACKGROUND: Microscopy is a routinely used technique for the diagnosis of canine tick-borne haemoparasitic diseases in various clinical laboratories worldwide. In an attempt to provide better diagnostic assay to the clients for effective management of ... ...

Abstract	BACKGROUND: Microscopy is a routinely used technique for the diagnosis of canine tick-borne haemoparasitic diseases in various clinical laboratories worldwide. In an attempt to provide better diagnostic assay to the clients for effective management of these diseases duplex real-time PCR assays were applied. METHODS AND RESULTS: Blood samples (n = 338) aseptically collected from suspected dogs of Central Plain Zone of Punjab state, India were subjected to SYBR Green based real-time duplex PCR assays for simultaneous detection of B. vogeli & E. canis and B. gibsoni & H. canis. Results revealed an overall prevalence rate of canine tick-borne haemoparasites as 54.1%, amongst which H. canis was the predominant (25.4%), followed by B. gibsoni (16.3%), E. canis (10.7%) and B. vogeli (1.8%). Sensitivity and specificity of the duplex assays ranged from 59.04 to 100.0% and 58.12 to 92.52%, respectively and their strength of agreement was ″fair″ with kappa value statistics. A significant (p < 0.05) association between prevalence of B. gibsoni, H. canis and E. canis infection with risk factors like sex, breed, season and location was recorded. The ancestral background of the field isolates of haemoparasites was also studied by phylogenetic analysis of their nucleotide sequences. CONCLUSIONS: SYBR Green dye based duplex real-time PCR assays proved to be highly sensitive, specific, rapid and affordable diagnostic tests for use by clinicians to save the life of pets.
Keywords	blood ; dogs ; dyes ; hemoparasites ; microscopy ; molecular biology ; phylogeny ; quantitative polymerase chain reaction ; India
Language	English
Dates of publication	2022-06
Size	p. 4451-4459.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-022-07286-4
Database	NAL-Catalogue (AGRICOLA)

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Article: Natural pigment from Monascus: The production and therapeutic significance

Chaudhary, Vishu / Katyal, Priya / Poonia, Anuj Kumar / Kaur, Jaspreet / Puniya, Anil Kumar / Panwar, Harsh

Journal of applied microbiology. 2022 July, v. 133, no. 1

2022

Abstract: OBJECTIVE: The present review highlights the advantages of using natural colorant over the synthetic one. We have discussed the fermentation parameters that can enhance the productivity of Monascus pigment on agricultural wastes. BACKGROUND: Food ... ...

Abstract	OBJECTIVE: The present review highlights the advantages of using natural colorant over the synthetic one. We have discussed the fermentation parameters that can enhance the productivity of Monascus pigment on agricultural wastes. BACKGROUND: Food industry is looking for natural colours because these can enhance the esthetic value, attractiveness, and acceptability of food while remaining nontoxic. Many synthetic food colours (Azorubine Carmoisine, quinoline) have been prohibited due to their toxicity and carcinogenicity. Increasing consumer awareness towards the food safety has forced the manufacturing industries to look for suitable alternatives. In addition to safety, natural colorants have been found to have nutritional and therapeutic significance. Among the natural colorants, microbial pigments can be considered as a viable option because of scalability, easier production, no seasonal dependence, cheaper raw materials and easier extraction. Fungi such as Monascus have a long history of safety and therefore can be used for production of biopigments. METHOD: The present review summarizes the predicted biosynthetic pathways and pigment gene clusters in Monascus purpureus. RESULTS: The challenges faced during the pilot‐scale production of Monascus biopigment and taming it by us of low‐cost agro‐industrial substrates for solid state fermentation has been suggested. CONCLUSION: Keeping in mind, therapeutic properties of Monascus pigments and their derivatives, they have huge potential for industrial and pharmaceutical application. APPLICATION: Though the natural pigments have wide scope in the food industry. However, stabilization of pigment is the greatest challenge and attempts are being made to overcome this by complexion with hydrocolloids or metals and by microencapsulation.
Keywords	Monascus purpureus ; aesthetics ; azorubine ; biosynthesis ; carcinogenicity ; food industry ; food safety ; hydrocolloids ; microencapsulation ; quinoline ; solid state fermentation ; therapeutics
Language	English
Dates of publication	2022-07
Size	p. 18-38.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	REVIEW
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/jam.15308
Database	NAL-Catalogue (AGRICOLA)

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