LIVIVO - Search results -

Search results

Result 1 - 10 of total 15559

Search options

Article ; Online: New Insights Into the Lineage-Specific Expansion and Functional Diversification of Lamprey AID/APOBEC Family.

Chen, Yan / Luo, Lingjie / Deng, Lisi / Tian, Xiaoxue / Chen, Shangwu / Xu, Anlong / Yuan, Shaochun

2022 Volume 13, Page(s) 822616

Abstract: The AID/APOBEC family which converts cytidine to uridine on RNA or DNA experienced dynamic expansion in primates in order to resist exogenous viruses and endogenous retrotransposons. Recently, expansion of AID/APOBEC-like homologs has also been observed ... ...

Abstract	The AID/APOBEC family which converts cytidine to uridine on RNA or DNA experienced dynamic expansion in primates in order to resist exogenous viruses and endogenous retrotransposons. Recently, expansion of AID/APOBEC-like homologs has also been observed in the extant jawless vertebrate lamprey. To reveal what causes such expansion and leads to the functional diversification of lamprey cytosine deaminases (CDAs), we reassessed the
MeSH term(s)	Animals ; Cytidine ; Cytidine Deaminase/genetics ; DNA/metabolism ; Lampreys/genetics ; Lampreys/metabolism ; Vertebrates/metabolism
Chemical Substances	Cytidine (5CSZ8459RP) ; DNA (9007-49-2) ; Cytidine Deaminase (EC 3.5.4.5)
Language	English
Publishing date	2022-03-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.822616
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Clinical and electroencephalographic characteristics of 34 infant with onset of epileptic spasms before three months of age.

Chen, Min / Yan, Lisi / Deng, Yu / Chen, Jin / Xie, Lingling / Hu, Yue / Hong, Siqi / Jiang, Li

Epilepsy & behavior : E&B

2023 Volume 149, Page(s) 109530

Abstract: Epileptic spasms (ES) occur mostly between age 3 months and 24 months. ES beginning before 3 months of age were called early-onset ES in previous studies. The aim of this study was to identify clinical and electroencephalographic characteristics of ... ...

Abstract	Epileptic spasms (ES) occur mostly between age 3 months and 24 months. ES beginning before 3 months of age were called early-onset ES in previous studies. The aim of this study was to identify clinical and electroencephalographic characteristics of patients with ES onset before 3 months of age. In total, 34 ES patients were retrospectively identified at Children's Hospital of Chongqing Medical University from January 1, 2020 to October 1, 2022. Our patients had diverse etiologies, including genetic (32.3 %), genetic-structural (11.8 %), structural-acquired (11.8 %), structural-congenital (8.8 %), and metabolic (5.9 %), with 29.4 % of patients having unknown etiology. Some patients experienced ES in clusters (either symmetrical or flexional) that occurred most often during awakening after sleep, and a minority of ES were characterized as isolated or asymmetrical, occurred during sleep, and could also manifest as relatively subtle. Approximately 35.3 % of patients also experienced other seizure types concurrently, including 10 focal seizures and 2 generalized seizures, and only half of the focal seizures had structural causes. The other seizure types occurred alone or sequentially with ES. Interictal electroencephalography revealed hypsarrhythmia or its variants, multifocal discharge, or burst suppression. 18 patients had no seizures lasting for more than 2 months, however, at the last follow-up visit, 5 of them had relapsed. All patients had different degrees of psychomotor retardation.
MeSH term(s)	Child ; Infant ; Humans ; Spasms, Infantile/complications ; Spasms, Infantile/diagnosis ; Retrospective Studies ; Seizures/diagnosis ; Seizures/etiology ; Electroencephalography/adverse effects ; Spasm
Language	English
Publishing date	2023-11-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2010587-3
ISSN	1525-5069 ; 1525-5050
ISSN (online)	1525-5069
ISSN	1525-5050
DOI	10.1016/j.yebeh.2023.109530
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5289: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Clinical and electroencephalography characteristics of 41 children with epileptic spasms onset after 1 year of age.

Yan, Lisi / Deng, Yu / Chen, Jin / Hu, Yue / Hong, Siqi / Jiang, Li

Epilepsy & behavior : E&B

2022 Volume 135, Page(s) 108902

Abstract: The incidence of epileptic spasms (ES) that begin after the first year of life is much lower than that before 1 year of age. The aim of this study was to identify clinical and electroencephalography (EEG) characteristics, etiologies, treatments, and ... ...

Abstract	The incidence of epileptic spasms (ES) that begin after the first year of life is much lower than that before 1 year of age. The aim of this study was to identify clinical and electroencephalography (EEG) characteristics, etiologies, treatments, and prognoses in pediatric patients with ES onset after 1 year of age. Forty-one children were retrospectively identified in Children's Hospital of Chongqing Medical University between January 1, 2020 and December 1, 2021. ES onset after 1 year of age have diverse presentations. Although most occur in clusters, are symmetrical and flexional, and occur frequently during awakening, some are characterized as isolated and asymmetrical, have a tonic component, and can also occur during sleep. The hypsarrhythmia variants and focal or multifocal discharges occur alternately in the interictal period, and the focal spikes and slow waves predominated in the unilateral temporal or frontotemporal areas. These patients had diverse etiologies, including structural (51.2 % of patients) and genetic (22.0 %) ones, and 11 patients (26.8 %) had an unknown etiology. No patients in our study had an infectious or immune-mediated etiology. Forty-eight percent of patients responded to hydrocortisone and/or adrenocorticotropic hormone. The efficacy of antiepileptic drug therapy was lower in patients who did not receive concurrent steroid therapy. However, ES onset after 1 year of age caused by a tumor, brain malformation, or other focal lesions, may be cured by focal cortical resection despite a lack of clearly localized EEG surface anomalies. Delays in motor, language, and cognitive development, or behavioral problems were observed in all but three patients.
MeSH term(s)	Adrenocorticotropic Hormone/therapeutic use ; Anticonvulsants/therapeutic use ; Child ; Electroencephalography ; Humans ; Hydrocortisone ; Infant ; Retrospective Studies ; Spasm ; Spasms, Infantile/diagnosis ; Spasms, Infantile/drug therapy
Chemical Substances	Anticonvulsants ; Adrenocorticotropic Hormone (9002-60-2) ; Hydrocortisone (WI4X0X7BPJ)
Language	English
Publishing date	2022-09-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2010587-3
ISSN	1525-5069 ; 1525-5050
ISSN (online)	1525-5069
ISSN	1525-5050
DOI	10.1016/j.yebeh.2022.108902
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5289: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Wogonin inhibits latent HIV-1 reactivation by downregulating histone crotonylation.

Zhang, Haitao / Cai, Jinfeng / Li, Chunna / Deng, Lisi / Zhu, Hongqiong / Huang, Ting / Zhao, Jiacong / Zhou, Jiasheng / Deng, Kai / Hong, Zhongsi / Xia, Jinyu

Phytomedicine : international journal of phytotherapy and phytopharmacology

2023 Volume 116, Page(s) 154855

Abstract: Background: Wogonin, a flavone isolated from Scutellaria baicalensis Georgi, is a commonly used phytochemical with anti-inflammatory and antitumor properties. However, the antiviral activity of wogonin against human immunodeficiency virus type 1 (HIV-1) ...

Abstract	Background: Wogonin, a flavone isolated from Scutellaria baicalensis Georgi, is a commonly used phytochemical with anti-inflammatory and antitumor properties. However, the antiviral activity of wogonin against human immunodeficiency virus type 1 (HIV-1) has not been reported. Purpose: The current study aimed to explore whether wogonin can suppress latent HIV-1 reactivation and the mechanism of wogonin in inhibiting proviral HIV-1 transcription. Methods: We assessed the effects of wogonin on HIV-1 reactivation using flow cytometry, cytotoxicity assay, quantitative PCR (qPCR), viral quality assurance (VQA), and western blot analysis. Results: Wogonin, a flavone isolated from S. baicalensis, significantly inhibited the reactivation of latent HIV-1 in cellular models and in primary CD4+ T cells from antiretroviral therapy (ART)-suppressed individuals ex vivo. Wogonin exhibited low cytotoxicity and long-lasting inhibition of HIV-1 transcription. Triptolide is a latency-promoting agent (LPA) that inhibits HIV-1 transcription and replication; wogonin had a stronger ability to inhibit HIV-1 latent reactivation than triptolide. Mechanistically, wogonin inhibited the reactivation of latent HIV-1 by inhibiting the expression of p300, a histone acetyltransferase, and decreasing the crotonylation of histone H3/H4 in the HIV-1 promoter region. Conclusion: Our study found that wogonin is a novel LPA that can inhibit HIV-1 transcription by HIV-1 epigenetic silencing, which could bear promising significance for future applications of HIV-1 functional cure.
MeSH term(s)	Humans ; Histones/metabolism ; HIV-1/physiology ; Virus Latency/physiology ; HIV Infections/drug therapy ; HIV Infections/metabolism ; CD4-Positive T-Lymphocytes
Chemical Substances	Histones ; triptolide (19ALD1S53J) ; wogonin (POK93PO28W)
Language	English
Publishing date	2023-05-03
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2023.154855
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4115: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Fueling sentinel node via reshaping cytotoxic T lymphocytes with a flex-patch for post-operative immuno-adjuvant therapy.

Li, Bei / Wang, Guohao / Miao, Kai / Zhang, Aiping / Sun, Liangyu / Yu, Xinwang / Lei, Josh Haipeng / Xie, Lisi / Yan, Jie / Li, Wenxi / Deng, Chu-Xia / Dai, Yunlu

Nature communications

2023 Volume 14, Issue 1, Page(s) 2518

Abstract: Clinical updates suggest conserving metastatic sentinel lymph nodes (SLNs) of breast cancer (BC) patients during surgery; however, the immunoadjuvant potential of this strategy is unknown. Here we leverage an immune-fueling flex-patch to animate ... ...

Abstract	Clinical updates suggest conserving metastatic sentinel lymph nodes (SLNs) of breast cancer (BC) patients during surgery; however, the immunoadjuvant potential of this strategy is unknown. Here we leverage an immune-fueling flex-patch to animate metastatic SLNs with personalized antitumor immunity. The flex-patch is implanted on the postoperative wound and spatiotemporally releases immunotherapeutic anti-PD-1 antibodies (aPD-1) and adjuvants (magnesium iron-layered double hydroxide, LDH) into the SLN. Genes associated with citric acid cycle and oxidative phosphorylation are enriched in activated CD8
MeSH term(s)	Female ; Mice ; Animals ; Sentinel Lymph Node/pathology ; Sentinel Lymph Node Biopsy ; CD8-Positive T-Lymphocytes ; T-Lymphocytes, Cytotoxic ; Neoplasm Recurrence, Local/pathology ; Adjuvants, Immunologic/therapeutic use ; Lymph Nodes/pathology
Chemical Substances	Adjuvants, Immunologic
Language	English
Publishing date	2023-05-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-38245-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Dolutegravir plus lamivudine versus efavirenz plus tenofovir disoproxil fumarate and lamivudine in antiretroviral-naive adults with HIV-1 infection.

Deng, Lisi / Li, Chunna / Chen, Ping / Luo, Xiaoqing / Zheng, Xinchun / Zhou, Lanlan / Zhou, Yi / Xia, Jinyu / Hong, Zhongsi

BMC infectious diseases

2022 Volume 22, Issue 1, Page(s) 17

Abstract: Background: Concerns regarding potential toxicity and drug-drug interactions during long-term treatment with three-drug active antiretroviral therapy (ART) regimens have been attracting increasing attention. We aimed to evaluate the efficacy and safety ... ...

Abstract	Background: Concerns regarding potential toxicity and drug-drug interactions during long-term treatment with three-drug active antiretroviral therapy (ART) regimens have been attracting increasing attention. We aimed to evaluate the efficacy and safety of dolutegravir (DTG) plus lamivudine (3TC) in ART-naive adults in China. Methods: This prospective observational cohort study enrolled HIV-naive inpatients treated with DTG + 3TC (2DR arm) or efavirenz (EFV) plus tenofovir disoproxil fumarate (TDF) and 3TC (3DR arm). There were no limits on baseline viral load. Inflammatory biomarkers were also investigated in the 2DR arm. Results: Between September 2019 and January 2020, 27 patients treated with DTG + 3TC and 28 patients treated with EFV + TDF + 3TC were enrolled in the study. At week 12, the proportion of patients with viral loads < 50 copies/mL in the 2DR arm was 81.5% (22/27) compared with 53.6% (15/28) in the 3DR arm (p < 0.01). At week 24, the proportion of patients with viral loads < 50 copies/mL in the 2DR arm was 100% (26/26) compared with 83.3% (20/24) in the 3DR arm (p < 0.05). Mean changes in CD4 cell counts from baseline at week 12 were 125.46 cells/µL in the 2DR arm and 41.20 cells/µL in the 3DR arm (p < 0.05). Mean changes in CD4 cell counts from baseline at week 24 were 209.68 cells/µL in the 2DR arm and 73.28 cells/µL in the 3DR arm (p < 0.05). Conclusions: DTG + 3TC achieved virologic suppression more rapidly than EFV + TDF + 3TC after 12 and 24 weeks. DTG + 3TC could represent an optimal regimen for advanced patients. Clinical Trial Registration ChiCTR1900027640 (22/November/2019).
MeSH term(s)	Alkynes ; Anti-HIV Agents/adverse effects ; Benzoxazines/adverse effects ; Cyclopropanes ; HIV Infections/drug therapy ; HIV-1 ; Heterocyclic Compounds, 3-Ring ; Humans ; Lamivudine/therapeutic use ; Oxazines ; Piperazines ; Prospective Studies ; Pyridones ; Tenofovir/therapeutic use
Chemical Substances	Alkynes ; Anti-HIV Agents ; Benzoxazines ; Cyclopropanes ; Heterocyclic Compounds, 3-Ring ; Oxazines ; Piperazines ; Pyridones ; Lamivudine (2T8Q726O95) ; Tenofovir (99YXE507IL) ; dolutegravir (DKO1W9H7M1) ; efavirenz (JE6H2O27P8)
Language	English
Publishing date	2022-01-04
Publishing country	England
Document type	Journal Article ; Observational Study
ISSN	1471-2334
ISSN (online)	1471-2334
DOI	10.1186/s12879-021-06991-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Differential Diagnosis of COVID-19 Pneumonia in Cancer Patients Received Radiotherapy.

Zeng, Qi / Tang, Caihua / Deng, Lisi / Li, Sheng / Liu, Jiani / Wang, Siyang / Shan, Hong

International journal of medical sciences

2020 Volume 17, Issue 16, Page(s) 2561–2569

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Adult ; Betacoronavirus ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/diagnostic imaging ; Diagnosis, Differential ; Esophageal Neoplasms/diagnostic imaging ; Esophageal Neoplasms/pathology ; Esophageal Neoplasms/radiotherapy ; Female ; Humans ; Lung Neoplasms/diagnostic imaging ; Lung Neoplasms/radiotherapy ; Male ; Middle Aged ; Nasopharyngeal Neoplasms/diagnostic imaging ; Nasopharyngeal Neoplasms/pathology ; Nasopharyngeal Neoplasms/radiotherapy ; Neoplasms/radiotherapy ; Neoplasms/virology ; Pandemics ; Pneumonia, Viral/diagnostic imaging ; Radiation Pneumonitis/diagnostic imaging ; Retrospective Studies ; SARS-CoV-2 ; Tomography, X-Ray Computed
Keywords	covid19
Language	English
Publishing date	2020-09-16
Publishing country	Australia
Document type	Case Reports ; Journal Article
ZDB-ID	2151424-0
ISSN	1449-1907 ; 1449-1907
ISSN (online)	1449-1907
ISSN	1449-1907
DOI	10.7150/ijms.46133
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: NFYC-37 promotes tumor growth by activating the mevalonate pathway in bladder cancer.

Liu, Zefu / Zheng, Xianchong / Chen, Jiawei / Zheng, Lisi / Ma, Zikun / Chen, Lei / Deng, Minhua / Tang, Huancheng / Zhou, Liwen / Kang, Tiebang / Wu, Yuanzhong / Liu, Zhuowei

Cell reports

2023 Volume 42, Issue 8, Page(s) 112963

Abstract: Dysregulation of transcription is a hallmark of cancer, including bladder cancer (BLCA). CRISPR-Cas9 screening using a lentivirus library with single guide RNAs (sgRNAs) targeting human transcription factors and chromatin modifiers is used to reveal ... ...

Abstract	Dysregulation of transcription is a hallmark of cancer, including bladder cancer (BLCA). CRISPR-Cas9 screening using a lentivirus library with single guide RNAs (sgRNAs) targeting human transcription factors and chromatin modifiers is used to reveal genes critical for the proliferation and survival of BLCA cells. As a result, the nuclear transcription factor Y subunit gamma (NFYC)-37, but not NFYC-50, is observed to promote cell proliferation and tumor growth in BLCA. Mechanistically, NFYC-37 interacts with CBP and SREBP2 to activate mevalonate pathway transcription, promoting cholesterol biosynthesis. However, NFYC-50 recruits more of the arginine methyltransferase CARM1 than NFYC-37 to methylate CBP, which prevents the CBP-SREBP2 interaction and subsequently inhibits the mevalonate pathway. Importantly, statins targeting the mevalonate pathway can suppress NFYC-37-induced cell proliferation and tumor growth, indicating the need for conducting a clinical trial with statins for treating patients with BLCA and high NFYC-37 levels, as most patients with BLCA have high NFYC-37 levels.
MeSH term(s)	Humans ; Mevalonic Acid/metabolism ; Hydroxymethylglutaryl-CoA Reductase Inhibitors ; RNA, Guide, CRISPR-Cas Systems ; Urinary Bladder Neoplasms/genetics ; Urinary Bladder Neoplasms/pathology ; Transcription Factors/metabolism
Chemical Substances	Mevalonic Acid (S5UOB36OCZ) ; Hydroxymethylglutaryl-CoA Reductase Inhibitors ; RNA, Guide, CRISPR-Cas Systems ; Transcription Factors
Language	English
Publishing date	2023-08-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2023.112963
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Effects of metformin and Exenatide on insulin resistance and AMPKα-SIRT1 molecular pathway in PCOS rats.

Tao, Xin / Cai, Lisi / Chen, Lei / Ge, Shuqi / Deng, Xuanying

Journal of ovarian research

2019 Volume 12, Issue 1, Page(s) 86

Abstract: Aims: This study was designed to evaluate the protective effects of AMPKα and SIRT1 on insulin resistance in PCOS rats, and to illuminate the underlying mechanisms.: Methods: An in vitro PCOS model was established by DHEA (6 mg/(100 g•d)), and the ... ...

Abstract	Aims: This study was designed to evaluate the protective effects of AMPKα and SIRT1 on insulin resistance in PCOS rats, and to illuminate the underlying mechanisms. Methods: An in vitro PCOS model was established by DHEA (6 mg/(100 g•d)), and the rats were randomly divided into the metformin group (MF group, n = 11), the exenatide group (EX group, n = 11), the PCOS group (n = 10), and the normal control group (NC group, n = 10). The MF group was administered MF 300 mg/(kg•d) daily. The EX group was subcutaneously injected EX 10μg/(kg•d) daily. After 4 weeks of continuous administration, fasting blood glucose and serum androgen, luteinizing hormone and other biochemical indicators were measured. Western and Real-time PCR were used to determine the expression of AMPKα and SIRT1 in the ovaries of each group. Results: After 4 weeks of drug intervention, compared with untreated PCOS group, EX group and MF group had visibly decreased body weight (222.64 ± 16.57, 218.63 ± 13.18 vs 238.30 ± 12.26 g, P = 0.026), fasting blood glucose (7.71 ± 0.72, 8.17 ± 0.54 vs 8.68 ± 0.47 mmol/L, P < 0.01), HOMA-IR (8.26 ± 2.50, 7.44 ± 1.23 vs 12.66 ± 1.44, P < 0.01) and serum androgen (0.09 ± 0.03, 0.09 ± 0.03 vs 0.53 ± 0.41 ng/ml, P < 0.01) and the expressions of AMPKα and SIRT11 were increased progressively (P < 0.05). Conclusions: Both metformin and exenatide can improve the reproductive and endocrine functions of rats with PCOS via the AMPKα-SIRT1 pathway, which may be the molecular mechanism for IR in PCOS and could possibly serve as a therapeutic target.
MeSH term(s)	AMP-Activated Protein Kinases/genetics ; Androgens/blood ; Animals ; Blood Glucose/drug effects ; Disease Models, Animal ; Exenatide/pharmacology ; Female ; Gene Expression Regulation/drug effects ; Humans ; Insulin Resistance/genetics ; Luteinizing Hormone/genetics ; Metformin/pharmacology ; Polycystic Ovary Syndrome/blood ; Polycystic Ovary Syndrome/drug therapy ; Polycystic Ovary Syndrome/genetics ; Rats ; Sirtuin 1/genetics
Chemical Substances	Androgens ; Blood Glucose ; Luteinizing Hormone (9002-67-9) ; Metformin (9100L32L2N) ; Exenatide (9P1872D4OL) ; Prkaa1 protein, rat (EC 2.7.11.1) ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; Sirt1 protein, rat (EC 3.5.1.-) ; Sirtuin 1 (EC 3.5.1.-)
Language	English
Publishing date	2019-09-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2455679-8
ISSN	1757-2215 ; 1757-2215
ISSN (online)	1757-2215
ISSN	1757-2215
DOI	10.1186/s13048-019-0555-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Knockdown of ZBTB11 impedes R-loop elimination and increases the sensitivity to cisplatin by inhibiting DDX1 transcription in bladder cancer.

Chen, Lei / Liu, Zefu / Tang, Huancheng / Zhou, Zhaohui / Chen, Jiawei / Ma, Zikun / Deng, Minhua / Li, Xiangdong / Wu, Yuanzhong / Zheng, Lisi / Zhou, Liwen / Zheng, Xianchong / Liu, Zhuowei

Cell proliferation

2022 Volume 55, Issue 12, Page(s) e13325

Abstract: Introduction: Bladder cancer (BC) is one of the most common malignant cancers, with poor prognosis and high incidence. Cisplatin is the standard chemotherapy for muscle invasive bladder cancer; however, chemotherapy resistance remains a major challenge. ...

Abstract	Introduction: Bladder cancer (BC) is one of the most common malignant cancers, with poor prognosis and high incidence. Cisplatin is the standard chemotherapy for muscle invasive bladder cancer; however, chemotherapy resistance remains a major challenge. Moreover, oncogenic signalling and the specific mechanisms underlying cisplatin resistance in BC remain largely unclear METHODS: In this study, RT-PCR, Western blot, immunofluorescence, and immunohistochemistry were used to measure gene and protein expression. Colony formation assay and flow cytometry were performed to evaluate the proliferation of BC cells. Gene set enrichment analysis was performed to identify the function in which ZBTB11 was involved. Luciferase and chromatin immunoprecipitation experiments were performed to determine the transcriptional regulation mechanism of ZBTB11. The effects of ZBTB11 on the malignant phenotypes of BC cells were examined in vitro and in vivo RESULTS: The results showed that ZBTB11 was remarkably upregulated in BC tissues, which was associated with poor prognosis in patients with BC. Furthermore, we found that knockdown of ZBTB11 remarkably inhibited the proliferation and tumorigenesis of BC cells by inducing apoptosis. Mechanistically, the knockdown of ZBTB11 transcriptionally inhibited DDX1 to suppress R-loop clearance, resulting in DNA damage in BC cells. Importantly, the ZBTB11/DDX1 axis is required for the chemotherapy resistance of BC cells to cisplatin CONCLUSION: Our findings not only reveal an underlying mechanism by which the ZBTB11/DDX1 axis promotes the tumorigenesis of BC but also provide a potential target for a combination strategy of cisplatin-based chemotherapy for BC.
MeSH term(s)	Humans ; Cisplatin/pharmacology ; Cisplatin/therapeutic use ; Urinary Bladder Neoplasms/drug therapy ; Urinary Bladder Neoplasms/genetics ; Urinary Bladder Neoplasms/pathology ; R-Loop Structures ; Cell Line, Tumor ; Carcinogenesis/genetics ; Gene Expression Regulation, Neoplastic ; MicroRNAs/genetics ; Cell Proliferation/genetics ; DEAD-box RNA Helicases/metabolism
Chemical Substances	Cisplatin (Q20Q21Q62J) ; MicroRNAs ; DDX1 protein, human (EC 3.6.1.-) ; DEAD-box RNA Helicases (EC 3.6.4.13)
Language	English
Publishing date	2022-08-26
Publishing country	England
Document type	Journal Article
ZDB-ID	1064202-x
ISSN	1365-2184 ; 0008-8730 ; 0960-7722
ISSN (online)	1365-2184
ISSN	0008-8730 ; 0960-7722
DOI	10.1111/cpr.13325
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 532: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito